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Q N Yu, Y B Guo, X Li, C L Li, W P Tan, X L Fan, Z L Qin, D Chen, W P Wen, S G Zheng, Q L Fu
BACKGROUND: Group 2 innate lymphoid cells (ILC2s) were closely associated with asthma. However, there were no perspective studies about the effects of glucocorticoid on ILC2s in asthma patients. Our objective was to perform a perspective study and evaluate the ILC2 activity after glucocorticoid therapy in asthma patients. METHODS: The asthma and asthma with allergic rhinitis patients were treated with glucocorticoid for 3 months. The circulating ILC2 levels were evaluated...
March 15, 2018: Allergy
Tuerhongjiang Tuxun, Shadike Apaer, Hai-Zhang Ma, Jin-Ming Zhao, Ren-Yong Lin, Tuerganaili Aji, Ying-Mei Shao, Hao Wen
Fluorodeoxyglucose (FDG) uptake by alveolar echinococcosis (AE) liver lesions is a signal of their metabolic activity and of disease progression. In order to find a surrogate marker for this status, we investigated whether parameters of the peripheral and/or periparasitic immune responses were associated with metabolic activity in a prospective case-control study of 30 AE patients and 22 healthy controls. Levels of 18 cytokines and chemokines, representative of innate and adaptive immune responses, were assessed in plasma and peripheral cells of two groups of patients with (MAAE) and without (MIAE) metabolically active lesions, and in the liver of MAAE patients...
March 13, 2018: Scientific Reports
Yrina Rochman, Krista Dienger-Stambaugh, Phoebe K Richgels, Ian P Lewkowich, Andrey V Kartashov, Artem Barski, Gurjit K Khurana Hershey, Warren J Leonard, Harinder Singh
Pathogenic T helper 2 (TH 2) cells, which produce increased amounts of the cytokines interleukin-5 (IL-5) and IL-13, promote allergic disorders, including asthma. Thymic stromal lymphopoietin (TSLP), a cytokine secreted by epithelial and innate immune cells, stimulates such pathogenic TH 2 cell responses. We found that TSLP signaling in mouse CD4+ T cells initiated transcriptional changes associated with TH 2 cell programming. IL-4 signaling amplified and stabilized the genomic response of T cells to TSLP, which increased the frequency of T cells producing IL-4, IL-5, and IL-13...
March 13, 2018: Science Signaling
Hong-Yi Li, Jing-Xia Meng, Zhen Liu, Xiao-Wen Liu, Yu-Guang Huang, Jing Zhao
Propofol, an intravenous anesthetic agent widely used in clinical practice, is the preferred anesthetic for asthmatic patients. This study was designed to determine the protective effect and underlying mechanisms of propofol on airway inflammation in a mast cell-dependent mouse model of allergic asthma. Mice were sensitized by ovalbumin (OVA) without alum and challenged with OVA three times. Propofol was given intraperitoneally 0.5 h prior to OVA challenge. The inflammatory cell count and production of cytokines in the bronchoalveolar lavage fluid (BALF) were detected...
March 12, 2018: Inflammation
Jamie Jennings-Gee, Sally Quataert, Tridib Ganguly, Ralph D'Agostino, Rajendar Deora, Purnima Dubey
The reemergence of pertussis or whooping cough in several countries highlights the need for better vaccines. Acellular pertussis vaccines (aPV) contain alum as the adjuvant and elicit Th2 biased immune responses that are less effective in protecting against infection than the reactogenic whole cell pertussis vaccines (wPV), which elicit primarily a Th1/Th17 response. An important goal for the field is to devise aPVs that will induce immune responses similar to wPV. We show that Bordetella Colonization Factor A (BcfA), an outer membrane protein from B...
March 12, 2018: Infection and Immunity
Andrea M Quinn, Allison R Williams, Teresa I Sivilli, Charles L Raison, Thaddeus W W Pace
Circulating concentrations of interleukin (IL)-6, an inflammatory biomarker widely assessed in humans to study the inflammatory response to acute psychological stress, have for decades been quantified using enzyme-linked immunosorbent assay (ELISA). However, biobehavioral researchers are increasingly using cytokine multiplex assays instead of ELISA to measure IL-6 and other cytokines. Despite this trend, multiplex assays have not been directly compared to ELISA for their ability to detect subtle stress-induced changes of IL-6...
March 13, 2018: Stress: the International Journal on the Biology of Stress
B Summer, S Ständer, P Thomas
BACKGROUND: Patch test (PT) is the gold standard to reveal Nickel (Ni) allergy. In vitro tests are under discussion. We aimed to establish a cytokine based in-vitro assay to detect Ni sensitization. METHODS: From 15 patients with positive (13f, 42-78yrs) and 15 with negative PT to Ni (controls, 3f, 31-82yrs) within a consecutive patient series peripheral blood mononuclear cells (PBMC) were obtained. 6 days stimulation with 3 concentrations of NiSO4 was done. Proliferative response (uptake of tritiated thymidine) and production of the cytokines IL-1β, IL-4, IL-5, IL-6, IL-8, IL-10, IL-17A, TNFα, IFNγ (cytometric bead array) were assessed...
March 10, 2018: Journal of the European Academy of Dermatology and Venereology: JEADV
Sung Gil Ha, Mythili Dileepan, Xiao Na Ge, Bit Na Kang, Yana G Greenberg, Amrita Rao, Girija Muralidhar, Lali Medina-Kauwe, Michael A Thompson, Christina M Pabelick, Scott M O'Grady, Savita P Rao, P Sriramarao
BACKGROUND: Altered epithelial physical and functional barrier properties along with Th1/Th2 immune dysregulation are features of allergic asthma. Regulation of junction proteins to improve barrier function of airway epithelial cells has the potential for alleviation of allergic airway inflammation. OBJECTIVE: To determine the immunomodulatory effect of knob protein of the adenoviral capsid on allergic asthma and investigate its mechanism of action on airway epithelial junction proteins and barrier function...
March 6, 2018: Journal of Allergy and Clinical Immunology
Christina Li-Ping Thio, Po-Yu Chi, Alan Chuan-Ying Lai, Ya-Jen Chang
BACKGROUND: Allergic asthma is characterized airway hyperreactivity (AHR) and inflammation driven by aberrant TH 2 response. Type 2 innate lymphoid cells (ILC2s) are a critical source of TH 2 cytokines IL-5 and IL-13 which promote acute asthma exacerbation. Short chain fatty acids (SCFAs) have been shown to attenuate T cell-mediated allergic airway inflammation. Their role in the regulation of ILC2-driven AHR and lung inflammation, however, remains unknown. OBJECTIVE: We investigated the immunomodulatory role of SCFAs in the regulation of ILC2-induced AHR and airway inflammation and delineated the mechanism involved...
March 6, 2018: Journal of Allergy and Clinical Immunology
A T Hastie, C Steele, C W Dunaway, W C Moore, B M Rector, E Ampleford, H Li, L C Denlinger, N Jarjour, D A Meyers, E R Bleecker
BACKGROUND: The release of various inflammatory mediators into the bronchial lumen is thought to reflect both the type and degree of airway inflammation, eosinophilic Th2, and Th9, or neutrophilic Th1, and Th17, in patients with asthma. AIMS: We investigated whether cytokines and chemokines differed in sputum from subjects with more severe compared with milder asthma, and if unbiased factor analysis of cytokine and chemokine groupings indicates specific inflammatory pathways...
March 9, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
Fumie Ihara, Daiju Sakurai, Syuji Yonekura, Tomohisa Iinuma, Ryoji Yagi, Toshioki Sakurai, Toshihiro Ito, Asaka Matsuura, Yuki Morimoto, Tomoyuki Arai, Satoshi Suzuki, Kazufumi Katayama, Toshinori Nakayama, Yoshitaka Okamoto
BACKGROUND: Although Th2 cells are well known to play important roles in allergic diseases including allergic rhinitis (AR), the factors that induce and sustain the pathogenesis of AR remain unclear. The recent development of sublingual immunotherapy (SLIT) is expected to allow changes to the underlying pathogenesis of AR. However, which Th2 cell subsets are important in house dust mite-induced AR (HDM-AR), the influence of SLIT on the pathogenic Th2 cells, and the association of Th2 cell subsets with SLIT efficacy have not been clarified...
March 8, 2018: Allergy
Difeng Fang, Kairong Cui, Gangqing Hu, Rama Krishna Gurram, Chao Zhong, Andrew J Oler, Ryoji Yagi, Ming Zhao, Suveena Sharma, Pentao Liu, Bing Sun, Keji Zhao, Jinfang Zhu
GATA-binding protein 3 (GATA3) acts as the master transcription factor for type 2 T helper (Th2) cell differentiation and function. However, it is still elusive how GATA3 function is precisely regulated in Th2 cells. Here, we show that the transcription factor B cell lymphoma 11b (Bcl11b), a previously unknown component of GATA3 transcriptional complex, is involved in GATA3-mediated gene regulation. Bcl11b binds to GATA3 through protein-protein interaction, and they colocalize at many important cis-regulatory elements in Th2 cells...
March 7, 2018: Journal of Experimental Medicine
Miho Ushijima, Takehito Uruno, Akihiko Nishikimi, Fumiyuki Sanematsu, Yasuhisa Kamikaseda, Kazufumi Kunimura, Daiji Sakata, Takaharu Okada, Yoshinori Fukui
A hallmark of humoral immune responses is the production of antibodies. This process involves a complex cascade of molecular and cellular interactions, including recognition of specific antigen by the B cell receptor (BCR), which triggers activation of B cells and differentiation into plasma cells (PCs). Although activation of the small GTPase Rac has been implicated in BCR-mediated antigen recognition, its precise role in humoral immunity and the upstream regulator remain elusive. DOCK2 is a Rac-specific guanine nucleotide exchange factor predominantly expressed in hematopoietic cells...
2018: Frontiers in Immunology
Bo Sun, Linnan Zhu, Yaling Tao, Hai-Xi Sun, Yang Li, Peng Wang, Yuzhu Hou, Yang Zhao, Xiaodong Zhang, Lianfeng Zhang, Ning Na, Yong Zhao
Neutrophils are involved in the pathogenesis of allergy. However, the contribution of the different functionally polarized neutrophils in allergy needs to be clarified. We sought to define the characteristics of interleukin (IL)-33-induced neutrophils and the involvement of this subset of polarized neutrophils in allergic pathogenesis. Freshly isolated neutrophils were treated with different cytokines and the cytokine expression levels were detected by real-time PCR. The gene expression profile of IL-33-induced neutrophils was determined by microarray assay...
March 5, 2018: Cellular & Molecular Immunology
Nidhi Malhotra, Juan Manuel Leyva-Castillo, Unmesh Jadhav, Olga Barreiro, Christy Kam, Nicholas K O'Neill, Francoise Meylan, Pierre Chambon, Ulrich H von Andrian, Richard M Siegel, Eddie C Wang, Ramesh Shivdasani, Raif S Geha
Atopic dermatitis is an allergic inflammatory skin disease characterized by the production of the type 2 cytokines in the skin by type 2 innate lymphoid cells (ILC2s) and T helper 2 (TH 2) cells, and tissue eosinophilia. Using two distinct mouse models of atopic dermatitis, we show that expression of retinoid-related orphan receptor α (RORα) in skin-resident T regulatory cells (Tregs ) is important for restraining allergic skin inflammation. In both models, targeted deletion of RORα in mouse Tregs led to exaggerated eosinophilia driven by interleukin-5 (IL-5) production by ILC2s and TH 2 cells...
March 2, 2018: Science Immunology
Tetsuo Miyake, Takashi Miyake, Makoto Sakaguchi, Hirokazu Nankai, Takahiro Nakazawa, Ryuichi Morishita
Transactivation of inflammatory and immune mediators in asthma is tightly regulated by nuclear factor κB (NF-κB) and signal transducer and activator of transcription 6 (STAT6). Therefore, we investigated the efficacy of simultaneous inhibition of NF-κB and STAT6 using a chimeric decoy strategy to prevent asthma exacerbation. The effects of decoy oligodeoxynucleotides were evaluated using an ovalbumin-induced mouse asthma model. Ovalbumin-sensitized mice received intratracheal administration of decoy oligodeoxynucleotides 3 days before ovalbumin challenge...
March 2, 2018: Molecular Therapy. Nucleic Acids
Sathisha Upparahalli Venkateshaiah, Akanksha Mishra, Murli Manohar, Alok K Verma, Priya Rajavelu, Rituraj Niranjan, Laurianne G Wild, Nereida A Parada, Uwe Blecker, Joseph A Lasky, Anil Mishra
The current studies demonstrate a critical role of IL-18 in transforming IL-5 generated naïve eosinophils into the distinct inflammatory CD101+ CD274+ expressing mature and activated tissue eosinophils that promote disease pathogenesis.
February 27, 2018: Journal of Allergy and Clinical Immunology
Shulian Wang, Jeff Campbell, Matthew H Stenmark, Paul Stanton, Jing Zhao, Martha M Matuszak, Randall K Ten Haken, Feng-Ming Kong
BACKGROUND AND PURPOSE: To study whether cytokine markers may improve predictive accuracy of radiation esophagitis (RE) in non-small cell lung cancer (NSCLC) patients. MATERIALS AND METHODS: A total of 129 patients with stage I-III NSCLC treated with radiotherapy (RT) from prospective studies were included. Thirty inflammatory cytokines were measured in platelet-poor plasma samples. Logistic regression was performed to evaluate the risk factors of RE. Stepwise Akaike information criterion (AIC) and likelihood ratio test were used to assess model predictions...
February 26, 2018: Radiotherapy and Oncology: Journal of the European Society for Therapeutic Radiology and Oncology
Dajeong Lee, Hyuk Soon Kim, Eunju Shin, Seon-Gil Do, Chong-Kil Lee, Young Mi Kim, Min Bum Lee, Keun Young Min, Jimo Koo, Su Jeong Kim, Seung Taek Nam, Hyun Woo Kim, Young Hwan Park, Wahn Soo Choi
An allergic reaction occurs when the immune system overreacts to harmless substance called allergen that gains access to the body. Food allergy is a hypersensitive immune reaction to food proteins and the number of patients with food allergy has recently increased. Aloe Vera is used for wellness and medicinal purposes. In particular, Aloe vera has been reported to enhance immunity. However, the effect of Aloe vera on food allergy is not yet known. In this study, we investigated the effects of processed Aloe vera gel (PAG) containing low molecular weight Aloe polysaccharide (AP) on ovalbumin (OVA)-induced food allergy in mice...
February 26, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Yan Zheng, Yu-Qing Rao, Jia-Kai Li, Yue Huang, Peiquan Zhao, Jing Li
AIM: To reveal age-related aqueous cytokine changes in human aqueous humor. METHODS: Aqueous humor was collected from 12 young children (3-6.5 years old) and 71 healthy adults (22-106 years old) with cataract but without other systemic or ocular disorders. Levels of 22 cytokines, chemokines and vascular endothelial growth factor (VEGF) were measured and analyzed. RESULTS: The following proteins showed significant increase from childhood to adult: interferon-gamma (IFN-γ), interleukin (IL)-13, IL-6, IL-12(p70), IL-10, CCL2, CCL3, CCL4, CXCL8, CXCL9, CXCL10, IFN-α2 and VEGF (all P <0...
2018: International Journal of Ophthalmology
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