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https://www.readbyqxmd.com/read/29217299/high-pd-l1-cd86-mfi-ratio-and-il-10-secretion-characterize-human-regulatory-dendritic-cells-generated-for-clinical-testing-in-organ-transplantation
#1
Alan F Zahorchak, Camila Macedo, David E Hamm, Lisa H Butterfield, Diana M Metes, Angus W Thomson
Human regulatory dendritic cells (DCreg) were generated from CD14 immunobead-purified or elutriated monocytes in the presence of vitamin D3 and IL-10. They exhibited similar, low levels of costimulatory CD80 and CD86, but comparatively high levels of co-inhibitory programed death ligand-1 (PD-L1) and IL-10 production compared to control immature DC (iDC). Following Toll-like receptor 4 ligation, unlike control iDC, DCreg resisted phenotypic and functional maturation and further upregulated PD-L1:CD86 expression...
September 14, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/29215791/protective-effects-of-carbonyl-iron-against-multiple-low-dose-streptozotocin-induced-diabetes-in-rodents
#2
Milica Vujicic, Tamara Saksida, Marija Mostarica Stojkovic, Neda Djedovic, Ivana Stojanovic, Stanislava Stosic-Grujicic
Particulate adjuvants have shown increasing promise as effective, safe and durable agents for the stimulation of immunity, or alternatively, the suppression of autoimmunity. Here we examined the potential of the adjuvant carbonyl iron (CI) for the modulation of organ-specific autoimmune disease - type 1 diabetes (T1D). T1D was induced by multiple low doses of streptozotocin (MLDS) that initiates beta cell death and triggers immune cell infiltration into the pancreatic islets. The results of this study indicate that the single in vivo application of CI to MLDS-treated DA rats, CBA/H mice, or C57BL/6 mice successfully counteracted the development of insulitis and hyperglycemia...
December 7, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29212160/hepatic-stroma-educated-regulatory-dcs-suppress-cd8-t-cell-proliferation-in-mice
#3
Qian Wang, Hao He, Dongwei Chen, Chao Wang, Yingping Xu, Wengang Song
Liver dendritic cells (DCs) display immunosuppressive activities and inhibit the CD4+ T cell response. The present study assessed whether and how liver DCs suppress CD8+ T cells. We found that bone marrow-derived mature DCs incubated with liver stromal cells were characterized by a longer life span, reduced CD11c, IA/IE, CD80, CD86, and CD40 expression, and increased CD11b expression. These unique liver stromal cell-educated mature DCs (LSed-DCs) stimulated CD8+ T cells to express CD25 and CD69, but inhibited their proliferation...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29208929/the-choice-of-cryopreservation-method-affects-immune-compatibility-of-human-cardiovascular-matrices
#4
Maria Schneider, Christof Stamm, Kelvin G M Brockbank, Ulrich A Stock, Martina Seifert
Conventional frozen cryopreservation (CFC) is currently the gold standard for cardiovascular allograft preservation. However, inflammation and structural deterioration limit transplant durability. Ice-free cryopreservation (IFC) already demonstrated matrix structure preservation combined with attenuated immune responses. In this study, we aim to explore the mechanisms of this diminished immunogenicity in vitro. First, we characterized factors released by human aortic tissue after CFC and IFC. Secondly, we analyzed co-cultures with human peripheral blood mononuclear cells, purified monocytes, T cells and monocyte-derived macrophages to examine functional immune effects triggered by the tissue or released cues...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29203862/diversification-of-human-plasmacytoid-predendritic-cells-in-response-to-a-single-stimulus
#5
Solana G Alculumbre, Violaine Saint-André, Jeremy Di Domizio, Pablo Vargas, Philemon Sirven, Pierre Bost, Mathieu Maurin, Paolo Maiuri, Maxime Wery, Mabel San Roman, Léa Savey, Maxime Touzot, Benjamin Terrier, David Saadoun, Curdin Conrad, Michel Gilliet, Antonin Morillon, Vassili Soumelis
Innate immune cells adjust to microbial and inflammatory stimuli through a process termed environmental plasticity, which links a given individual stimulus to a unique activated state. Here, we report that activation of human plasmacytoid predendritic cells (pDCs) with a single microbial or cytokine stimulus triggers cell diversification into three stable subpopulations (P1-P3). P1-pDCs (PD-L1+CD80-) displayed a plasmacytoid morphology and specialization for type I interferon production. P3-pDCs (PD-L1-CD80+) adopted a dendritic morphology and adaptive immune functions...
December 4, 2017: Nature Immunology
https://www.readbyqxmd.com/read/29197260/susceptibility-of-dendritic-cells-from-individuals-with-schistosomiasis-to-infection-by-leishmania-braziliensis
#6
Diego Mota Lopes, Tarcísio Vila Verde S de Almeida, Robson da Paixão de Souza, Luís Eduardo Viana Ribeiro, Brady Page, Jamille de Souza Fernandes, Edgar M Carvalho, Luciana Santos Cardoso
Coinfection with leishmaniasis and schistosomiasis has been associated with increased time to healing of cutaneous lesions of leishmaniasis. The objective of this study was to evaluate the effect of Leishmania braziliensis infection on co-cultures of monocyte-derived dendritic cells (MoDCs) with autologous lymphocytes from patients with schistosomiasis and patients with cutaneous leishmaniasis. MoDCs were differentiated from peripheral blood monocytes, isolated by magnetic beads, infected with L. braziliensis, and co-cultured with autologous lymphocytes...
November 29, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29194317/gut-epithelial-cell-derived-exosomes-trigger-post-trauma-immune-dysfunction
#7
Mitsuaki Kojima, Todd W Costantini, Brian P Eliceiri, Theresa W Chan, Andrew Baird, Raul Coimbra
INTRODUCTION: Exosomes are extracellular vesicles that act as endogenous mediators of the immune response. We have previously shown that exosomes released into mesenteric lymph (ML) following trauma/hemorrhagic shock (T/HS) induce pro-inflammatory cytokine production in macrophages and are involved in the pathogenesis of post-shock acute lung injury. However, the cellular origin of ML exosomes and their role in the post-trauma immune response remains unclear. We hypothesized that exosomes released from damaged-intestinal epithelial cells (IECs) contribute to post-trauma immune dysfunction by altering the function of dendritic cells (DCs), key regulators of the adaptive immunity...
November 21, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/29181416/cd80-expressed-by-cd8-t-cells-contributes-to-pd-l1-induced-apoptosis-of-activated-cd8-t-cells
#8
Meagan R Rollins, Rachel M Gibbons Johnson
Tumor cells are capable of limiting antitumor CD8+ T cell responses through their cell surface expression of PD-L1. In addition to PD-1 expressed by CD8+ T cells, PD-L1 also binds to CD80 expressed by CD8+ T cells. The influence of the PD-L1/CD80 interaction on CD8+ T cell function has not been fully characterized, so we sought to investigate the impact of the PD-L1/CD80 interaction on PD-L1-induced apoptosis of activated CD8+ T cells. We found that CD8+ T cells that lacked CD80 expression got activated to the same extent as wild-type CD8+ T cells, but when cultured with anti-CD3 and PD-L1/Fc protein, activated CD8+ T cells that lacked CD80 expression survived better than activated wild-type CD8+ T cells...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/29179438/valproate-hampers-podocyte-acquisition-of-immune-phenotypes-via-intercepting-the-gsk3%C3%AE-facilitated-nfkb-activation
#9
Pei Wang, Sijie Zhou, Yan Ge, Minglei Lu, Zhangsuo Liu, Rujun Gong
Glomerular podocytes are able to transdifferentiate under disease conditions, acquire de novo immune phenotypes and behave as immunocompetent cells, like phagocytes or antigen-presenting cells. Upon stimulation with lipopolysaccharide (LPS), a prototypical pathogen-associated molecular pattern, podocytes demonstrated de novo expression of a variety of NFkB-dependent immune molecules that are pivotal for immune response, including major histocompatibility complex (MHC) class II, costimulatory molecule CD80, lysosomal protease cathepsin L as well as CC chemokine ligand 2 and 5, ultimately resulting in podocyte dysfunction, characterized by cellular shrinkage, a spindle-like or asterlike cell shape and impairment of actin cytoskeleton integrity...
October 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/29170044/differential-gene-expression-in-mycobacterium-bovis-challenged-monocyte-derived-macrophages-of-cattle
#10
Sanjeev Kumar Shukla, Shubhra Shukla, Anuj Chauhan, Sarvjeet, Rehan Khan, Lakshya Veer Singh, Naveen Sharma, Chandan Prakash, Ajay Vir Singh, Manjit Panigrahi
A functional genomics approach was used to examine the immune response for transcriptional profiling of PBMC M. bovis infected cattle and healthy control cattle to stimulation with bovine tuberculin (purified protein derivative PPD-b). Total cellular RNA was extracted from non-challenged control and M. bovis challenged MDM for all animals at intervals of 6 h post-challenge, in response to in-vitro challenge with M. bovis (multiplicity of infection 2:1) and prepared for global gene expression analysis using the Agilent Bovine (V2) Gene Expression Microarray, 8 × 60 K...
November 20, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29164976/ibrutinib-and-idelalisib-block-immunophenotypic-changes-associated-with-the-adhesion-and-activation-of-cll-cells-in-the-tumor-microenvironment
#11
Yandong Shen, O Giles Best, Stephen P Mulligan, Richard I Christopherson
The lymph node and bone marrow microenvironments promote the survival and proliferation of CLL cells. Defining the immunophenotype of CLL cells from the tumor microenvironment may help to better understand the mechanisms of action of current therapies and identify novel drug targets. Significant changes in the levels of 25 CD antigens were identified using the DotScan™ antibody microarray following CLL-cell culture with CD40L-expressing fibroblasts. Ibrutinib or idelalisib countered the change in expression of 11 of these antigens (CD23, CD27, CD53, CD58, CD71, CD80, CD84, CD97, CD126, CD150, and FMC7), which have known roles in cell activation and adhesion...
November 22, 2017: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/29164392/maturation-and-cytokine-pattern-of-human-dendritic-cells-in-response-to-different-yeasts
#12
Silvia Boschi Bazan, Barbara Walch-Rückheim, Manfred J Schmitt, Frank Breinig
Activated dendritic cells (DC) induce and polarize T-cell responses by expression of distinct maturation markers and cytokines. This study systematically investigated the capacity of different biotechnically relevant yeast species and strains including Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis, Pichia pastoris, Hansenula polymorpha, Yarrowia lipolytica, and Candida glabrata to initiate maturation of human DC. As important prerequisite for T-cell activation, all yeasts were shown to effectively induce, though to a different extent, the expression of the activation marker CD83, the co-stimulatory molecules CD80, CD86, CD54, CD58, and CD40, as well as the antigen-presenting molecules MHCs I and II...
November 21, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/29163502/regulating-immunogenicity-and-tolerogenicity-of-bone-marrow-derived-dendritic-cells-through-modulation-of-cell-surface-glycosylation-by-dexamethasone-treatment
#13
Kevin Lynch, Oliver Treacy, Jared Q Gerlach, Heidi Annuk, Paul Lohan, Joana Cabral, Lokesh Joshi, Aideen E Ryan, Thomas Ritter
Dendritic cellular therapies and dendritic cell vaccines show promise for the treatment of autoimmune diseases, the prolongation of graft survival in transplantation, and in educating the immune system to fight cancers. Cell surface glycosylation plays a crucial role in the cell-cell interaction, uptake of antigens, migration, and homing of DCs. Glycosylation is known to change with environment and the functional state of DCs. Tolerogenic DCs (tDCs) are commonly generated using corticosteroids including dexamethasone, however, to date, little is known on how corticosteroid treatment alters glycosylation and what functional consequences this may have...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29162324/modulation-of-human-macrophage-activity-by-ascaris-antigens-is-dependent-on-macrophage-polarization-state
#14
Sara Almeida, Peter Nejsum, Andrew R Williams
Parasitic worms (helminths) are known to actively modulate host immune responses and inflammation. The aim of this study was to investigate if adult body fluid (ABF) from the helminth Ascaris suum has immunomodulatory effects on different subtypes of human monocyte-derived macrophages (Mɸ) in vitro. Mɸs were exposed to A. suum ABF at different stages of their differentiation and/or polarization. Mɸ were first differentiated from monocytes into either uncommitted (M-), classically activated (M(GM-CSF)) or alternatively activated (M(M-CSF)) phenotypes and then stimulated with lipopolysaccharide (LPS)...
November 15, 2017: Immunobiology
https://www.readbyqxmd.com/read/29158419/ultraviolet-b-induced-maturation-of-cd11b-type-langerin-dendritic-cells-controls-the-expansion-of-foxp3-regulatory-t-cells-in-the-skin
#15
Sayuri Yamazaki, Mizuyu Odanaka, Akiko Nishioka, Saori Kasuya, Hiroaki Shime, Hiroaki Hemmi, Masaki Imai, Dieter Riethmacher, Tsuneyasu Kaisho, Naganari Ohkura, Shimon Sakaguchi, Akimichi Morita
Skin dendritic cells (DCs) are divided into several subsets with distinctive functions. This study shows a previously unappreciated role of dermal CD11b-type Langerin(-) DCs in maintaining immunological self-tolerance after UVB exposure. After UVB exposure, dermal CD11b-type Langerin(-) DCs upregulated surface CD86 expression, induced proliferation of Foxp3(+) regulatory T (Treg) cells without exogenous Ags, and upregulated a set of genes associated with immunological tolerance. This Treg-expansion activity was significantly hampered by CD80/CD86 blockade in vivo...
November 20, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29157299/the-similarities-between-smdcs-and-regdcs-in-alleviating-the-immune-injury-caused-by-transplantation-of-hepatocytes-differentiated-from-escs
#16
Cheng Zhang, Wenwei Liao, Bing Cai, Furong Liu, Qiong Ke, Xiaofeng Zhu, Xiaoshun He, Anbin Hu
BACKGROUND: This study aimed to investigate the tolerogenic mechanisms induced by semimature dendritic cells (smDCs) and regulatory dendritic cells (regDCs) after transplantation of hepatocytes differentiated from mouse embryonic stem cells (ESCs) and to confirm the low immunogenicity of hepatocytes differentiated from ESCs. METHODS: Green fluorescent protein-labeled ESCs collected from 129 mice were cultured to differentiate into hepatocytes. smDCs and regDCs were cultured in vitro...
November 21, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29156562/biodegradable-chitosan-decreases-the-immune-response-to-trichinella-spiralis-in-mice
#17
Klaudia Brodaczewska, Natalia Wolaniuk, Katarzyna Lewandowska, Katarzyna Donskow-Łysoniewska, Maria Doligalska
The purpose of this study was to evaluate the potential of chitosan units released during natural degradation of the polymer to activate the immune system against T. spiralis infection. High molecular weight chitosan was injected intraperitoneally into C57BL/6 mice. Flow cytometry and cytokine concentration, measured by ELISA, were used to characterize peritoneal cell populations during T. spiralis infection. The strong chemo-attractive properties of chitosan caused considerable infiltration into the peritoneal cavity of CD11b⁺ cells, with reduced expression of MHC class II, CD80, CD86, Dectin-1 or CD23 receptors in comparison to T...
November 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29155997/immune-activation-and-benefit-from-avelumab-in-ebv-positive-gastric-cancer
#18
Anshuman Panda, Janice M Mehnert, Kim M Hirshfield, Greg Riedlinger, Sherri Damare, Tracie Saunders, Michael Kane, Levi Sokol, Mark N Stein, Elizabeth Poplin, Lorna Rodriguez-Rodriguez, Ann W Silk, Joseph Aisner, Nancy Chan, Jyoti Malhotra, Melissa Frankel, Howard L Kaufman, Siraj Ali, Jeffrey S Ross, Eileen P White, Gyan Bhanot, Shridar Ganesan
Response to immune checkpoint therapy can be associated with a high mutation burden, but other mechanisms are also likely to be important. We identified a patient with metastatic gastric cancer with meaningful clinical benefit from treatment with the anti-programmed death-ligand 1 (PD-L1) antibody avelumab. This tumor showed no evidence of high mutation burden or mismatch repair defect but was strongly positive for presence of Epstein-Barr virus (EBV) encoded RNA. Analysis of The Cancer Genome Atlas gastric cancer data (25 EBV+, 80 microsatellite-instable [MSI], 310 microsatellite-stable [MSS]) showed that EBV-positive tumors were MSS...
November 15, 2017: Journal of the National Cancer Institute
https://www.readbyqxmd.com/read/29153616/a-role-for-b-cells-in-facilitating-defense-against-an-nk-cell-sensitive-lung-metastatic-tumor-is-revealed-by-stress
#19
Harlan P Jones, Beau Aldridge, Katherine Boss-Williams, Jay M Weiss
Stressors impair immune defenses and pose risks among cancer patients. Natural Killer cells are not the sole immune defense against tumor development. Utilizing an NK-sensitive tumor model, this study evaluated immune effects to stress and determined whether lung metastasis resulted from B cells' inability to augment tumorlytic function. Lung metastasis directly correlated with delayed lung B cell accumulation compared to NK, and T cells. Decreased interleukin-12 cytokine and CD80(+) molecule expression by B cells correlated with decreased tumor lysis and increased tumor development...
December 15, 2017: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/29151286/-experimental-study-of-glioma-stem-cell-mediated-immune-tolerance-in-tumor-microenvironment
#20
T Xie, J W Ma, B Liu, J Dong, Q Huang
Objective: To investigate the tumor microenvironment of immune tolerance induced by glioma stem cells (GSC). Methods: Human GSC SU3 cells transfected with red fluorescent protein (SU3-RFP) gene were implanted into the brain, subcutis (armpit and foot), liver and abdominal cavity of transgenic green fluorescence protein (GFP) nude mice to establish RFP(+) /GFP(+) dual fluorescence solid tumor model. The re-cultured cells derived from implanted tumor tissues, SU3-RFP cells co-cultured with peritoneal fluid of transgenic GFP nude mice and malignant ascites of tumor-bearing mice were observed by fluorescence microscopy and real-time video image tracing to analyze the microenvironment of immune tolerance mediated by RFP(+) /GFP(+) implanted tumor...
November 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
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