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https://www.readbyqxmd.com/read/28303856/effect-of-matrix-metallopeptidase-13-on-the-function-of-mouse-bone-marrow-derived-dendritic-cells
#1
Xiao-Dong Li, Xin-Rui Zhang, Zhi-Hao Li, Yang Yang, Duo Zhang, Heng Zheng, Shu-Ying Dong, Juan Chen, Xian-Dong Zeng
BACKGROUND: Dendritic cells are professional antigen-presenting cells found in an immature state in epithelia and interstitial space, where they capture antigens such as pathogens or damaged tissue. Matrix metallopeptidase 13 (MMP-13), a member of the collagenase subfamily, is involved in many different cellular processes and is expressed in murine bone marrow-derived dendritic cells (DCs). The function of MMP-13 in DCs is not well understood. Here, we investigated the effect of MMP-13 on DC maturation, apoptosis, and phagocytosis...
March 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28303416/microrna-24-modulates-staphylococcus-aureus-induced-macrophage-polarization-by-suppressing-chi3l1
#2
Zhang Jingjing, Zhang Nan, Wu Wei, Guo Qinghe, Wang Weijuan, Wang Peng, Wang Xiangpeng
Macrophages play a crucial role in host innate anti-Staphylococcus aureus defense, which is tightly regulated by multiple factors, including microRNAs. A recent study showed that miR-24 plays an important role in macrophage polarization. Here, we investigated the biological function of miR-24 in S. aureus-stimulated macrophages. The results revealed that miR-24 expression was significantly decreased in both human and mouse macrophage cell lines with S. aureus stimulation in a time-dependent manner. Moreover, miR-24 overexpression significantly decreased the production of M1 phenotype markers, such as IL-6, iNOS, TNF-α, CD86, and CD80, whereas it increased the production of M2 markers, such as Arg1, CCL17, CCL22, CD163, and CD206, in S...
March 16, 2017: Inflammation
https://www.readbyqxmd.com/read/28302543/immunomodulatory-effects-of-pidotimod-in-adults-with-community-acquired-pneumonia-undergoing-standard-antibiotic-therapy
#3
D Trabattoni, M Clerici, S Centanni, M Mantero, M Garziano, F Blasi
The morbidity and mortality of community-acquired pneumonia (CAP) are still elevated and two aspects seem to contribute to a worse outcome: an uncontrolled inflammatory reaction and an inadequate immune response. Adjuvants, including corticosteroids and intravenous immunoglobulins, have been proposed to counterbalance these effects but their efficacy is only partial. We examined the immunomodulatory activity of Pidotimod (PDT), a synthetic dipeptide molecule in adult patients hospitalized for CAP. Sixteen patients with a diagnosis of CAP and a PSI score III or IV and/or a CURB-65 0-2 were randomized to receive either levofloxacin 500 mg b...
March 13, 2017: Pulmonary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28299384/impairment-of-systemic-dha-synthesis-affects-macrophage-plasticity-and-polarization-implications-for-dha-supplementation-during-inflammation
#4
Emanuela Talamonti, Anna M Pauter, Abolfazl Asadi, Alexander W Fischer, Valerio Chiurchiù, Anders Jacobsson
Docosahexaenoic acid (DHA) is an omega-3 fatty acid obtained from the diet or synthesized from alpha-linolenic acid through the action of fatty acid elongases (ELOVL) and desaturases. DHA plays important roles in the central nervous system as well as in peripheral organs and is the precursor of several molecules that regulate resolution of inflammation. In the present study, we questioned whether impaired synthesis of DHA affected macrophage plasticity and polarization both in vitro and in vivo models. For this we investigated the activation status and inflammatory response of bone marrow-derived M1 and M2 macrophages obtained from mice deficient of Elovl2 (Elovl2(-/-)), a key enzyme for DHA synthesis in mammals...
March 15, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28297658/multiscale-modeling-of-complex-formation-and-cd80-depletion-during-immune-synapse-development
#5
István P Sugár, Jayajit Das, Ciriyam Jayaprakash, Stuart C Sealfon
The mechanisms that discriminate self- and foreign antigen before T cell activation are unresolved. As part of the immune system's adaptive response to specific infections or neoplasms, antigen-presenting cells (APC) and effector T cells form transcellular molecular complexes. CTLA4 expression on regulatory or effector T cells reduces T cell activation. The CTLA4 transendocytosis hypothesis proposes that CTLA4 depletes CD80 and CD86 proteins from the APC membrane, rendering the APC incapable of activating T cells...
March 14, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28294314/generation-of-precursor-immature-and-mature-murine-b1-cell-lines-from-c-myc-bcl-xl-overexpressing-pre-bi-cells
#6
Inge Wolf, Corinne Bouquet, Friederike Naumann, Fritz Melchers
Deregulated expression of c-myc and bcl-xL is long known to generate transformed B cells in humans and mice. We overexpressed these genes to induce in vitro and in vivo differentiation of fetal liver-derived mouse pre-BI cells to B1-lineage pre-BII-like, immature and mature B-cell lines and to Ig-secreting cells. In vitro, doxycycline-controlled c-myc/bcl-xL-overexpressing CD19(+) CD93(+) c-kikt(+) IgM(-) pre-BI cells differentiate to and survive as CD19(+) CD93(+) c-kit(-) IgM(+) immature B1 cells. Timed CpG-stimulation of these oncogene-overexpressing pre-B or immature B1 cells generates either CD19(+) CD93(low) c-kit(-) IgM(-) SLC(-) pre-BII-like, or IgM(+) MHCII(+) CD73(+) CD80(+) CD40(+) mature B1-cell lines and IgM-secreting B1 cells in vitro and fixes their state of differentiation...
March 10, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/28293237/dc-derived-il-10-modulates-pro-inflammatory-cytokine-production-and-promotes-induction-of-cd4-il-10-regulatory-t-cells-during-plasmodium-yoelii-infection
#7
Katharina Loevenich, Kristina Ueffing, Simone Abel, Matthias Hose, Kai Matuschewski, Astrid M Westendorf, Jan Buer, Wiebke Hansen
The cytokine IL-10 plays a crucial role during malaria infection by counteracting the pro-inflammatory immune response. We and others demonstrated that Plasmodium yoelii infection results in enhanced IL-10 production in CD4(+) T cells accompanied by the induction of an immunosuppressive phenotype. However, it is unclear whether this is a direct effect caused by the parasite or an indirect consequence due to T cell activation by IL-10-producing antigen-presenting cells. Here, we demonstrate that CD11c(+)CD11b(+)CD8(-) dendritic cells (DCs) produce elevated levels of IL-10 after P...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28293065/the-combination-of-mbp-and-bcg-induced-dendritic-cell-maturation-through-tlr2-tlr4-promotes-th1-activation-in-vitro-and-vivo
#8
LiNa Jiang, GuoMu Liu, WeiHua Ni, NanNan Zhang, Jing Jie, Fei Xie, GuiXiang Tai
To explore whether TLR2/TLR4 could be involved in the maturation of dendritic cells and polarization of CD4(+) T cells induced by dendritic cells stimulated with MBP and BCG, in vitro and in vivo experiments using TLR2(-/-) or TLR4(-/-) mice were employed. MBP and BCG elevated CD80, CD86 and MHC class II expressed on dendritic cells and increased IL-12 protein, induced DC maturation, and indirectly promoted Th1 activation. Moreover, MBP and BCG upregulated costimulatory molecules on DCs in a TLR2- and TLR4-dependent manner...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28291636/correlation-between-messenger-rna-expression-and-protein-expression-of-immune-checkpoint-associated-molecules-in-bladder-urothelial-carcinoma-a-retrospective-study
#9
Constance Le Goux, Diane Damotte, Sophie Vacher, Mathilde Sibony, Nicolas Barry Delongchamps, Anne Schnitzler, Benoit Terris, Marc Zerbib, Ivan Bieche, Géraldine Pignot
OBJECTIVES: Immunotherapy for bladder cancer seems to have promising results. Here, we evaluated the association between messenger RNA (mRNA) and protein levels and possible prognostic value of the programmed cell death 1/programmed cell death ligand 1 (PD-1/PD-L1) and cytotoxic T-lymphocyte-associated protein 4 (CTLA4) immune checkpoint pathways during bladder carcinogenesis. METHODS AND MATERIALS: Tumor samples were obtained from 155 patients (84 with muscle-invasive bladder cancer [MIBC], and 71 non-muscle-invasive bladder cancer [NMIBC]) and normal bladder tissue from 15 patients...
March 10, 2017: Urologic Oncology
https://www.readbyqxmd.com/read/28288151/pentoxifylline-modulates-lps-induced-hyperinflammation-in-monocytes-of-preterm-infants-in-vitro
#10
Simone S Schüller, Lukas Wisgrill, Elisabeth Herndl, Andreas Spittler, Elisabeth Förster-Waldl, Kambis Sadeghi, Boris W Kramer, Angelika Berger
BACKGROUND: Pentoxifylline (PTX), a methylxanthine derivate with immunomodulating properties, has been used as adjunctive treatment in severe neonatal sepsis. The aim of the study was to investigate PTX's anti-inflammatory effects on LPS stimulated monocytes of preterm neonates in vitro compared to monocytes from term infants and adult controls. METHODS: Whole cord blood and control adult blood samples were incubated with LPS and PTX. The expression of surface markers, phagocytosis, cytokine secretion and Toll-like-receptor (TLR)4 signaling of monocytes were assessed by flow cytometry...
March 13, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28275176/human-bronchial-epithelium-orchestrates-dendritic-cell-activation-in-severe-asthma
#11
Delphine Gras, Asuncion Martinez-Anton, Arnaud Bourdin, Celine Garulli, Laure de Senneville, Isabelle Vachier, Joana Vitte, Pascal Chanez
The innate immune response is impaired in asthma, with increased epithelial release of C-X-C motif chemokine ligand (CXCL)8, interleukin (IL)-33 and thymic stromal lymphopoietin (TSLP). We hypothesised that dendritic cells might modulate the hyperresponsive epithelium in severe asthma.For this purpose, we investigated epithelial-dendritic crosstalk in normal and diseased conditions, and because ultrafine particulate matter may affect asthmatic airways, we investigated its impact on this crosstalk. Air-liquid interface cultures of human bronchial epithelial cells (HBEC) of control subjects (cHBEC) or severe asthma patients (saHBEC) were co-cultured with monocyte-derived dendritic cells (moDC)...
March 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/28275140/antibody-independent-function-of-human-b-cells-contributes-to-antifungal-t-cell-responses
#12
Rui Li, Ayman Rezk, Hulun Li, Jennifer L Gommerman, Alexandre Prat, Amit Bar-Or
Fungal infections (e.g., Candida albicans) can manifest as serious medical illnesses, especially in the elderly and immune-compromised hosts. T cells are important for Candida control. Whether and how B cells are involved in antifungal immunity has been less clear. Although patients with agammaglobulinemia exhibit normal antifungal immunity, increased fungal infections are reported following B cell-depleting therapy, together pointing to Ab-independent roles of B cells in controlling such infections. To test how human B cells may contribute to fungal-associated human T cell responses, we developed a novel Ag-specific human T cell/B cell in vitro coculture system and found that human B cells could induce C...
March 8, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28274366/pemphigus-vulgaris-and-pemphigus-foliaceus-determined-by-cd86-and-ctla4-polymorphisms
#13
Srdjan Tanasilovic, Svetlana Popadic, Ljiljana Medenica, Dusan Popadic
Pemphigus vulgaris (PV) and pemphigus foliaceus (PF) are rare autoimmune blistering diseases with presumed T-cell-dependent pathology. Activation of naïve T cells is dependent on antigen recognition, subsequent signaling through the T-cell receptor complex (signal 1), and various other interactions of T cells with antigen presenting cells that may be collectively designated as signal 2, which is unconditionally required for T-cell activation both in response to infection and to autoantigens. Among the best described interactions contributing to signal 2 are those mediated by B7 family molecules, such as CD80 and CD86 with their ligands; CD28, providing activation signals; and cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4), conferring inhibition...
March 2017: Clinics in Dermatology
https://www.readbyqxmd.com/read/28265274/tlr7-deficiency-leads-to-tlr8-compensative-regulation-of-immune-response-against-jev-in-mice
#14
Muhammad Awais, Ke Wang, Xianwu Lin, Wenjie Qian, Nan Zhang, Chong Wang, Kunlun Wang, Ling Zhao, Zhen F Fu, Min Cui
Japanese encephalitis virus (JEV) is a highly fatal pathogen to human beings. Toll-like receptor 7 (TLR7) plays a role as the first host defense against most single-stranded RNA flaviviruses. This study aims to investigate the role of TLR7 in inducing adaptive immune response in mice against JEV. In vitro and in vivo studies were conducted to examine the expression of toll-like receptors (TLRs) in mice. After JEV infection, physical parameters of mice (survival rate and body weight) were evaluated, and organs or cells were collected for further analysis...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28261215/tnf-blockade-maintains-an-il-10-phenotype-in-human-effector-cd4-and-cd8-t-cells
#15
Ceri A Roberts, Lucy E Durham, Veerle Fleskens, Hayley G Evans, Leonie S Taams
CD4(+) and CD8(+) effector T cell subpopulations can display regulatory potential characterized by expression of the prototypically anti-inflammatory cytokine IL-10. However, the underlying cellular mechanisms that regulate expression of IL-10 in different T cell subpopulations are not yet fully elucidated. We recently showed that TNF inhibitors (TNFi) promote IL-10 expression in human CD4(+) T cells, including IL-17(+) CD4(+) T cells. Here, we further characterized the regulation of IL-10 expression via blockade of TNF signaling or other cytokine/co-stimulatory pathways, in human T cell subpopulations...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28261212/%C3%AE-2%C3%A2-1-fructans-modulate-the-immune-system-in-vivo-in-a-microbiota-dependent-and%C3%A2-independent-fashion
#16
Floris Fransen, Neha M Sahasrabudhe, Marlies Elderman, Margaret Bosveld, Sahar El Aidy, Floor Hugenholtz, Theo Borghuis, Ben Kousemaker, Simon Winkel, Christa van der Gaast-de Jongh, Marien I de Jonge, Mark V Boekschoten, Hauke Smidt, Henk A Schols, Paul de Vos
It has been shown in vitro that only specific dietary fibers contribute to immunity, but studies in vivo are not conclusive. Here, we investigated degree of polymerization (DP) dependent effects of β2→1-fructans on immunity via microbiota-dependent and -independent effects. To this end, conventional or germ-free mice received short- or long-chain β2→1-fructan for 5 days. Immune cell populations in the spleen, mesenteric lymph nodes (MLNs), and Peyer's patches (PPs) were analyzed with flow cytometry, genome-wide gene expression in the ileum was measured with microarray, and gut microbiota composition was analyzed with 16S rRNA sequencing of fecal samples...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28260295/-experimental-study-on-the-immune-response-of-fusion-tumor-vaccine-of-hepg2-and-dendritic-cells-in-vitro
#17
Y B Pang, B Y Cui, J He, X P Huang, W Liang, L Q Li, X L Luo
Objective: To estimate the immune response of HepG2/dendritic cell (DC) fusion cells vaccines against HepG2 cells in vitro. Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors by Ficoll-Hypaque density-gradient centrifugation.Then DC were obtain from PBMCs by culturing in medium containing granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) for 5 days.DC and HepG2 fusion cells were induced by polythyleneglycol (PEG). The fusion cells were examined under fluorescence microscope by labeling DCs and HepG2 with green and red fluorescein, respectively, and then the fusion rates were analyzed by flow cytometry...
February 21, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28240301/pplase-of-dermatophagoides-farinae-promotes-ovalbumin-induced-airway-allergy-by-modulating-the-functions-of-dendritic-cells-in-a-mouse-model
#18
Hui Wang, Lihua Mo, Xiaojun Xiao, Shu An, Xiaoyu Liu, Jinge Ba, Weifang Wu, Pixin Ran, Pingchang Yang, Zhigang Liu
Our previous studies revealed that many proteins in addition to the known allergens of D. farinae have not been fully characterized. We observed that Pplase did not respond to serum collected from patients sensitized to D. farinae. In a mouse model, Pplase significantly enhanced airway hyperresponsiveness (AHR) and Th2 responses induced by ovalbumin (OVA) compared with mice treated with OVA alone. Moreover, exposure to Pplase significantly increased the expression of IRF4, CD80, CD83, MHCII and TNF-α in DC2...
February 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28239788/expression-of-surface-molecules-in-human-mesenchymal-stromal-cells-co-cultured-with-nucleated-umbilical-cord-blood-cells
#19
Yu A Romanov, E E Balashova, N E Volgina, N V Kabaeva, T N Dugina, G T Sukhikh
We studied the expression of different classes of surface molecules (CD13, CD29, CD40, CD44, CD54, CD71, CD73, CD80, CD86, CD90, CD105, CD106, CD146, HLA-I, and HLA-DR) in mesenchymal stromal cells from human umbilical cord and bone marrow during co-culturing with nucleated umbilical cord blood cells. Expression of the majority of surface markers in both types of mesenchymal stromal cells was stable and did not depend on the presence of the blood cells. Significant differences were found only for cell adhesion molecules CD54 (ICAM-1) and CD106 (VCAM-1) responsible for direct cell-cell contacts with leukocytes and only for bone marrow derived cells...
February 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28235615/polysaccharide-purified-from-ganoderma-atrum-induced-activation-and-maturation-of-murine-myeloid-derived-dendritic-cells
#20
Hui Wang, Qiang Yu, Shao-Ping Nie, Quan-Dan Xiang, Ming-Ming Zhao, Shi-Yu Liu, Ming-Yong Xie, Shun-Qi Wang
Ganoderma atrum (G. atrum), a member of the genus Ganoderma, is an edible and medicinal fungus. In this study, we investigated the direct and indirect effects of G. atrum polysaccharide (PSG-1) on dendritic cells (DCs). Firstly, flow cytometric and ELISA analysis showed that PSG-1 increased cell surface molecule expression of MHC-II, CD80 and CD86, and enhanced the production of IL-12 p70, IL-6, IL-10, RANTES, MIP-1α and MCP-1 in DCs. PSG-1-treated DCs promoted the proliferation of splenic T lymphocyte of mouse in mixed lymphocyte reaction...
February 21, 2017: Food and Chemical Toxicology
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