Hongji Yan, Song Zhang, Jin He, Yanbin Yin, Xiumei Wang, Xiongbiao Chen, Fuzhai Cui, Yu Li, Yongzhan Nie, Weiming Tian
The interactions between cancer cells and the extracellular matrix (ECM) are important with respect to a number of cell behavoirs, yet remain unclear. In this study, self-assembled monolayers with different terminal chemical groups (hydroxyl (-OH), carboxyl (-COOH), animo (-NH2), mercapto (-SH), and methyl (-CH3)) were employed as substrates for the culture of MCF-7 cells to examine effects on cell behavior. Cell spreading was investigated by scanning electron microscopy, tallin expression by immunofluorescence, proliferation rate by counting cell numbers, cell cycle by flow cytometry, metabolism by high-performance liquid chromatography and cell migration by live cell imaging...
June 2013: Biomedical Materials