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Microbiology keratitis

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https://www.readbyqxmd.com/read/29150218/keratitis-secondary-to-fusarium-spp-in-spain-2012-2014
#1
M A Mosquera Gordillo, N Barón Cano, A Garralda Luquin, C López Gutiérrez, E Mengual Verdú, G Trujillo Cabrera, J M Garrido Fierro, J Lamarca Mateu, J C Sánchez España, P Marticorena Álvarez, R Feijoó Lera, S Martín Nalda, T Marti Huguet, V Gacía Conca
OBJECTIVE: To describe the clinical-epidemiological characteristics of a case series of fungal keratitis associated with Fusarium spp.., in Spain during the years 2012 to 2014. METHODS: A retrospective study of a case series was conducted on a systematic random sample of 10% of patients identified in Health Centres of provincial capitals (n=250). The centres were asked whether they had been presented with cases of Fusarium spp. keratitis characterised by microbiological methods...
November 14, 2017: Archivos de la Sociedad Española de Oftalmología
https://www.readbyqxmd.com/read/29133630/non-contact-lens-related-acanthamoeba-keratitis
#2
REVIEW
Prashant Garg, Paavan Kalra, Joveeta Joseph
The purpose of the study is to describe epidemiology, clinical features, diagnosis, and treatment of Acanthamoeba keratitis (AK) with special focus on the disease in nonusers of contact lenses (CLs). This study was a perspective based on authors' experience and review of published literature. AK accounts for 2% of microbiology-proven cases of keratitis. Trauma and exposure to contaminated water are the main predisposing factors for the disease. Association with CLs is seen only in small fraction of cases. Contrary to classical description experience in India suggests that out of proportion pain, ring infiltrate, and radial keratoneuritis are seen in less than a third of cases...
November 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29111996/microbial-keratitis-after-accelerated-corneal-collagen-cross-linking-in-keratoconus
#3
Prafulla K Maharana, Pranita Sahay, M Sujeeth, Deepali Singhal, Anubha Rathi, Jeewan S Titiyal, Namrata Sharma
PURPOSE: To assess the pattern of microbial keratitis after accelerated corneal collagen cross-linkage (aCXL) in patients with keratoconus. METHOD: The medical records of cases of keratoconus that underwent aCXL from June 2014 to May 2017 were reviewed. Cases that developed microbial keratitis after aCXL were included in the study. The clinical, microbiological profile and the treatment outcomes were evaluated. RESULTS: Of 532 eyes that underwent aCXL, 7 cases developed microbial keratitis during the study period...
November 2, 2017: Cornea
https://www.readbyqxmd.com/read/29111995/diagnostic-evaluation-of-co-occurrence-of-acanthamoeba-and-fungi-in-keratitis-a-preliminary-report
#4
Anita Raghavan, Shaffie Baidwal, Prabhu Vijayaraghavan, Sakthi Rajeswari, Revathi Rajaraman, Narendran Venkatapathy, Sunitha Menon, Ram Rammohan
PURPOSE: To test the hypothesis that the coexistence of Acanthamoeba with other forms of microbial keratitis, especially fungal keratitis (FK), is more prevalent than suspected. METHODS: A prospective diagnostic study whereby patients presenting with stromal keratitis were additionally tested for Acanthamoeba, irrespective of the initial diagnosis. In addition to the routine workup with Gram stain, KOH mount, and cultures on blood agar and potato dextrose agar, nonnutrient agar was included...
November 14, 2017: Cornea
https://www.readbyqxmd.com/read/29064157/inflammation-and-oxidative-stress-in-corneal-tissue-in-experimental-keratitis-due-to-fusarium-solani-amelioration-following-topical-therapy-with-voriconazole-and-epigallocatechin-gallate
#5
Vasanthakumar Vasantha Ruban, Archana P Teresa, Mahalingam Sundararajan, Pitchairaj Geraldine, Philip Aloysius Thomas
Combined antifungal and antioxidant therapy may help to reduce oxidative stress in fungal keratitis. Experimental Fusarium solani keratitis was induced by application of F. solani conidia to scarified cornea (right eye) of 16 rabbits (another 4 rabbits were negative controls [Group I]). Five days later, F. solani-infected animals began receiving hourly topical saline alone (Group II), voriconazole (10 mg/ml) alone (Group III), epigallocatechingallate (EGCG, 10 mg/ml) alone (Group IV) or voriconazole and EGCG (Group V)...
October 24, 2017: Mycoses
https://www.readbyqxmd.com/read/29040115/keratitis-after-post-kala-azar-dermal-leishmaniasis
#6
Aditya Pradhan, Samar Basak, Tuhin Chowdhury, Ayan Mohanta, Ananya Chatterjee
PURPOSE: To report 2 cases of keratitis occurring after post-kala-azar dermal leishmaniasis (PKDL) in immunocompetent patients. METHODS: Case report and literature review. RESULTS: Two male patients presented with a dense stromal infiltrate in the temporal and superior aspects of the cornea, respectively. Microbiological workup in both patients did not show any offending organism. Both patients were undergoing treatment for PKDL in the form of oral miltefosine...
October 13, 2017: Cornea
https://www.readbyqxmd.com/read/28993066/clinical-and-microbiological-profile-of-infectious-keratitis-in-an-area-of-madrid-spain
#7
Soraya Mediero, Ana Boto de Los Bueis, Karina Spiess, Mariana Díaz-Almirón, Almudena Del Hierro Zarzuelo, Isabel Villalaín Rodes, Adela García Perea
INTRODUCTION: To study antibiotic susceptibility in bacterial keratitis (BK), its profile over 10 years and its influence on ophthalmological practice. METHODS: Retrospective review of BK with positive corneal scraping over a 10-year period. Risk factors for keratitis, visual acuity (VA), empirical topical treatment, corneal infection characteristics and outcomes were analyzed for BK due to Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Pseudomonas aeruginosa and Propionibacterium acnes...
October 6, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28965660/assessment-of-confocal-microscopy-for-the-diagnosis-of-polymerase-chain-reaction-positive-acanthamoeba-keratitis-a-case-control-study
#8
Sophie De Craene, Juliette Knoeri, Cristina Georgeon, Philippe Kestelyn, Vincent M Borderie
PURPOSE: To determine in vivo confocal microscopy diagnostic criteria to diagnose Acanthamoeba keratitis (AK) using polymerase chain reaction (PCR) as the reference diagnostic technique. DESIGN: Retrospective case-control study. Data were recorded prospectively and analyzed retrospectively. PARTICIPANTS: Fifty patients with PCR-positive AK (study group) and 50 patients with bacterial, fungal, viral, or immune keratitis featuring negative Acanthamoeba PCR results (control group)...
September 28, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28938380/bacterial-keratitis-in-toronto-a-16-year-review-of-the-microorganisms-isolated-and-the-resistance-patterns-observed
#9
Alex L C Tam, Elie Côté, Mario Saldanha, Alejandro Lichtinger, Allan R Slomovic
PURPOSE: To review the incidence, distribution, current trends, and resistance patterns of bacterial keratitis isolates in Toronto over the past 16 years. METHODS: Microbiology records of suspected bacterial keratitis that underwent a diagnostic corneal scraping and cultures from January 1, 2000, through December 31, 2015, were retrospectively reviewed. The distribution of the main isolated pathogens and in vitro laboratory minimum inhibitory concentration testing results were used to identify resistance patterns...
December 2017: Cornea
https://www.readbyqxmd.com/read/28936051/bacterial-contamination-of-multi-dose-eye-drops-at-ophthalmology-department-university-of-gondar-northwest-ethiopia
#10
Asegedech Tsegaw, Asamere Tsegaw, Tefera Abula, Yared Assefa
PURPOSE: Ophthalmic solutions used for both diagnostic and therapeutic purposes were found to be contaminated with bacteria pathogens and caused serious ocular infections such as keratitis and endophthalmitis. The objective was to assess the magnitude and pattern of bacterial contamination of multi-dose ophthalmic medications and investigate the drug susceptibility pattern of the isolates in the Department of Ophthalmology at Gondar University Teaching Hospital. METHODS: A total of 100 ophthalmic medications in-use by patients and eye-care workers have been taken and cultured for potential bacterial contamination in the Microbiology Department after 1 week and >1 week of use...
April 2017: Middle East African Journal of Ophthalmology
https://www.readbyqxmd.com/read/28903964/clinical-profile-of-pythium-keratitis-perioperative-measures-to-reduce-risk-of-recurrence
#11
Shweta Agarwal, Geetha Iyer, Bhaskar Srinivasan, Mamta Agarwal, Shobha Panchalam Sampath Kumar, Lily K Therese
PURPOSE: To report the clinical profile and role of perioperative adjunctive measures to reduce the risk of recurrence in Pythium insidiosum keratitis. METHODS: Retrospective analysis of 10 eyes of 10 patients with P. insidiosum keratitis. Diagnosis was confirmed by PCR DNA sequencing. RESULTS: 7out of 10 patients were from urban locales, and none had any obvious history of injury with vegetative matter and were being treated for fungal keratitis...
September 13, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28902722/diagnostic-approach-to-ocular-infections-using-various-techniques-from-conventional-culture-to-next-generation-sequencing-analysis
#12
Hiroshi Eguchi, Fumika Hotta, Tomomi Kuwahara, Haruyuki Imaohji, Chika Miyazaki, Miou Hirose, Shunji Kusaka, Masahiko Fukuda, Yoshikazu Shimomura
Ocular infection is caused by both endogenous (resident) and exogenous (environmental) microbes. As the ocular surface interacts with both outer environment and its own resident microbiota, clinical ocular samples are predicted to contain a diverse set of microorganisms. Microscopy of sample smears is an important step in the diagnostic process of infectious diseases to interpret the culture results. Traditional culture techniques have several limitations in the detection and/or identification of uncharacterized bacteria of environmental origin...
November 2017: Cornea
https://www.readbyqxmd.com/read/28879155/cylindrocarpon-lichenicola-keratomycosis-in-nigeria-the-challenge-of-limited-access-to-effective-antimicrobials
#13
Emmanuel O Irek, Temitope O Obadare, Patrick A Udonwa, Olajumoke Laoye, Oyekola V Abiri, Adenike O Adeoye, Aaron O Aboderin
INTRODUCTION: We report a rare cause of keratitis, due to Cylindrocarpon lichenicola, in a farmer with keratomycosis. Despite the acknowledged virulence of this fungus, a suitable antifungal for its management was not accessible. CASE PRESENTATION: A 67-year-old farmer presented with a two-week history of pain, mucopurulent discharge, redness and a corneal ulcer with a visual acuity of hand movement in the right eye. With a working diagnosis of infective keratitis, corneal scrapings were taken under a slit lamp biomicroscope for microbiological testing...
2017: African Journal of Laboratory Medicine
https://www.readbyqxmd.com/read/28739646/koh-mount-as-an-aid-in-the-management-of-infectious-keratitis-at-secondary-eye-care-centre
#14
Varsha M Rathi, Monica Thakur, Savitri Sharma, Rohit Khanna, Prashant Garg
AIMS: To report the clinical outcome of infectious keratitis managed after doing 10% KOH mount of corneal smears and reporting done by an ophthalmologist in the secondary eye care centre in South India. METHODS: 103 consecutive cases of microbial keratitis were studied. Inclusion criteria were presence of corneal infiltrate on slit lamp biomicroscopy. An ophthalmologist carried out microbiological evaluation of 10% KOH mount of corneal scrapings. No cultures were done at secondary centres...
November 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28708218/microbial-characteristics-of-posttraumatic-infective-keratitis
#15
Blanche X Lim, Victor T C Koh, Manotosh Ray
PURPOSE: To determine the demographics, risk factors, clinical and microbiological characteristics, and treatment outcome of posttraumatic infective keratitis. METHODS: Consecutive patients with posttraumatic infective keratitis presenting to the Ophthalmology Department of a tertiary referral hospital in Singapore between March 2012 and March 2016 were prospectively identified. A standardized data collection form was used to document patient demographics, microbiological diagnosis, antibiotic sensitivity, and pretreatment and posttreatment ocular characteristics...
July 8, 2017: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/28659135/bilateral-morganella-morganii-keratitis-in-a-patient-with-facial-topical-corticosteroid-induced-rosacea-like-dermatitis-a-case-report
#16
Bei Zhang, Fei Pan, Kejian Zhu
BACKGROUND: Bilateral keratitis rarely occurs in individuals without predisposing factors. Here we describe the clinical course of a patient who developed a bilateral keratitis caused by Morganella. morganii which might be associated with long term using of topical corticosteroids-containing preparations on the face. CASE PRESENTATION: A 52-year-old female patient presented with marked bilateral corneal infiltration and hypopyon without any usual predisposing factors for bilateral infectious keratitis...
June 28, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28658756/unusual-severe-extra-intestinal-manifestations-of-a-common-enteric-pathogen-aeromonas-spp
#17
Barnini Banerjee, Mridula Madiyal, Lingadakai Ramchandra, Chiranjay Mukhopadhyay, Rahul Garg, Kiran Chawla
INTRODUCTION: Aeromonas spp. are halophilic, Gram-negative bacilli. They are widely distributed in the soil and aquatic environment and have been associated with various extra-intestinal infections, such as skin and soft-tissue infections, meningitis, bacteraemia etc. The most common species associated with extra-intestinal infection is Aeromonas hydrophila. AIM: To get the overview of clinical presentations, underlying predisposing factors associated with the extra-intestinal infections caused by Aeromonas species and their antimicrobial susceptibility pattern...
May 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28632575/clinical-profile-etiology-and-outcome-of-infantile-ocular-trauma-a-developing-country-perspective
#18
Vedang D Shah, Madhu Uddaraju, Amitabh Singh, Rashmi Ranjan Das
OBJECTIVE: The aim of this article was to study the clinical profile, etiology, and outcome of infantile ocular trauma in a developing country setting. METHODS: A retrospective study on corneal trauma in infants (≤12 months old) was undertaken in a tertiary care hospital during a 2-year period. An analysis of clinical profile, etiology, microbiological profile, clinical course, and outcome was studied. RESULTS: Seventy-six infants were included...
June 20, 2017: Pediatric Emergency Care
https://www.readbyqxmd.com/read/28631638/clinicomicrobiological-profile-of-endophthalmitis-a-10-year-experience-in-a-tertiary-care-center-in-north-india
#19
Gita Satpathy, Niranjan Nayak, Meenakshi Wadhwani, Pradeep Venkwatesh, Atul Kumar, Yograj Sharma, Vishnu Sreenivas
PURPOSE: To determine the clinicomicrobiological profile of infectious agents and their antibiotic susceptibility in different type of endophthalmitis. METHODS: A retrospective review of clinical and microbiological records from January 2001 to December 2010, was performed in 1110 patients diagnosed with different type of endophthalmitis (postoperative, posttraumatic, endogenous and post keratitis) to record the demographic details, clinical presentations; microbiological agents isolated with their antimicrobial sensitivity pattern...
April 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28603237/-contact-lens-related-fusarium-keratitis-a-case-report
#20
Silvia Amadasi, Giovanni Federico Pelliccioli, Paolo Colombini, Annalisa Bonomini, Claudio Farina, Filomena Pietrantonio, Palmino Pedroni
We aim to highlight the key factors for a good outcome of fungal keratitis. We describe a case of contact lens-related Fusarium keratitis in a young girl. After identification of Fusarium spp under direct microscopic examination and in culture, a prolonged treatment with topic natamycin 5% was started and administered for five months with restitutio ad integrum of the eye. Prompt microbiological diagnosis and a specific and prolonged treatment are essential for correct management of Fusarium keratitis.
June 1, 2017: Le Infezioni in Medicina
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