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Acanthamoeba keratitis

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https://www.readbyqxmd.com/read/29916123/effectiveness-of-sampling-methods-employed-for-acanthamoeba-keratitis-diagnosis-by-culture
#1
Laura Muiño, Donoso Rodrigo, Rodrigo Villegas, Pablo Romero, Daniel E Peredo, Rafael A Vargas, Daniela Liempi, Antonio Osuna, María Isabel Jercic
PURPOSE: This retrospective, observational study was designed to evaluate the effectiveness of the sampling methods commonly used for the collection of corneal scrapes for the diagnosis of Acanthamoeba keratitis (AK) by culture, in terms of their ability to provide a positive result. METHODS: A total of 553 samples from 380 patients with suspected AK received at the Parasitology Section of the Public Health Institute of Chile, between January 2005 and December 2015, were evaluated...
June 18, 2018: International Ophthalmology
https://www.readbyqxmd.com/read/29885816/a-report-of-10-patients-with-acanthamoeba-keratitis
#2
E A Alfonso-Muñoz, M J Roig-Revert, E Fernández-López, M Hernández-Díaz, R Araujo-Miranda, C Peris-Martínez
CLINICAL CASES: Cases are presented of 10 patients with Acanthamoeba keratitis treated between 2008 and 2017. All were contact lens wearers. All of them received treatment with a biguanide combined with a diamidine. In 3 cases the infestation did not exceed the superficial stroma, responding to topical treatment. In 7, the infection reached the deep stroma, with 6 of these cases requiring penetrating keratoplasty (PKP), 3 of them therapeutic PKP because of perforation risk or ocular spreading...
June 6, 2018: Archivos de la Sociedad Española de Oftalmología
https://www.readbyqxmd.com/read/29885293/polyhexamethylene-biguanide-and-chloroquine-induce-programmed-cell-death-in-acanthamoeba-castellanii
#3
Eun-Kyung Moon, Hyun-Seo Choi, Hyun-Hee Kong, Fu-Shi Quan
Several chemotherapeutic drugs have been described as amoebicidal agents acting against Acanthamoeba trophozoites and cysts. However, the underlying mechanism of action is poorly characterized. Here, we describe programmed cell death (PCD) in A. castellanii induced by polyhexamethylene biguanide (PHMB) and chloroquine. We used four types of amoebicidal agents including 0.02% PHMB, 0.02% chlorhexidine digluconate, 100 μM chloroquine, and 100 μM 2,6-dichlorobenzonitrile to kill Acanthamoeba trophozoites and cysts...
June 6, 2018: Experimental Parasitology
https://www.readbyqxmd.com/read/29881423/-in-vitro-activity-of-trigonella-foenum-graecum-seeds-against-a-clinical-strain-of-acanthamoeba-genotype-t4
#4
Samira Dodangeh, Maryam Niyyati, Mohammad Kamalinejad, Jacob Lorenzo-Morales, Abdolali Moshfe, Ali Haghighi, Eznolah Azargashb
Acanthamoeba keratitis (AK) is a sight-threatening infection of the cornea disease that often presents with a lengthy and not fully effective treatment. Current therapeutic options against Acanthamoeba are not very effective against the cyst. Calibrated trophozoite/cyst suspension was incubated with the same volume of serial dilutions of the Trigonella foenum graecum aqueous extract (200, 250, 350, 450, 600, and 750 mg/mL) in microcentrifuge tubes and mixed by pipetting up and down. After that, the tubes were incubated at 26 ºC for 24, 48, and 72 h...
2018: Iranian Journal of Pharmaceutical Research: IJPR
https://www.readbyqxmd.com/read/29867132/toxic-effects-of-selected-proprietary-dry-eye-drops-on-acanthamoeba
#5
Ines Sifaoui, María Reyes-Batlle, Atteneri López-Arencibia, Olfa Chiboub, Javier Rodríguez-Martín, Pedro Rocha-Cabrera, Basilio Valladares, José E Piñero, Jacob Lorenzo-Morales
Amoebae of the genus Acanthamoeba are ubiquitous protists that have been isolated from many sources such as soils, water and the air. They are responsible for infections including fatal encephalitis and a severe keratitis in humans. To date, there is no satisfactorily effective therapeutic agent against this pathogen and the infections it causes are exacerbated by the existence of a resistant cyst stage produced by this amoeba. As dry eye syndrome is a risk factor for Acanthamoeba keratitis, we aimed to evaluate the anti-Acanthamoeba activity of a variety of proprietary eye drops intended to treat dry eye syndrome...
June 4, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29859227/acanthamoeba-keratitis-an-emerging-disease-among-microbial-keratitis-in-the-cap-bon-region-of-tunisia
#6
W Zbiba, N Ben Abdesslem
INTRODUCTION: The aim of this study was to describe the prevalence of AK among microbial keratitis as well as their clinical features and to compare their risk factors to those of other infectious keratitis, over the last five years in a referral center in the region of Cap Bon, Tunisia, North Africa. METHODS: A retrospective review of the charts of 230 patients (230 eyes) diagnosed with presumed infectious keratitis between January 2011 and December 2016 at the department of ophthalmology of the university hospital of Nabeul in Tunisia...
May 30, 2018: Experimental Parasitology
https://www.readbyqxmd.com/read/29847494/acanthamoeba-keratitis-are-recent-cases-more-severe
#7
Mehdi Roozbahani, Kristin M Hammersmith, Christopher J Rapuano, Parveen K Nagra, Qiang Ed Zhang, Sophia Y Siu
PURPOSE: To identify a recent change in disease severity and visual outcomes of patients with Acanthamoeba keratitis (AK) at Wills Eye Hospital (WEH). METHODS: A retrospective chart review was performed of all cases diagnosed with AK at WEH between January 1, 2009, and December 31, 2015. Failure of treatment was defined as having a final best-corrected visual acuity worse than 20/100 and/or requiring keratoplasty. The patients were grouped into 2 outcome categories: successfully treated and those who failed treatment; and prognostic factors associated with failure status were evaluated...
May 25, 2018: Cornea
https://www.readbyqxmd.com/read/29780912/orbital-implant-exposure-after-acanthamoeba-panophthalmitis
#8
Meredith S Baker, Amanda C Maltry, Nasreen A Syed, Richard C Allen
Purpose: Acanthamoeba is a protozoa that can lead to severe ocular disease and sequelae. Although intraocular Acanthamoeba infection is rare, the following case demonstrates an unusual presentation of recurrent Acanthamoeba infection in a 30 year old contact lens wearing male. Observations: After presenting with recurrent Acanthamoeba keratitis and undergoing various treatments, the patient developed nodular scleritis, which evolved into panophthalmitis, and ultimately, required enucleation...
June 2018: American Journal of Ophthalmology Case Reports
https://www.readbyqxmd.com/read/29745319/acanthamoeba-keratitis-current-status-and-urgent-research-priorities
#9
Naveed Ahmed Khan, Ayaz Anwar, Ruqaiyyah Siddiqui
BACKGROUND: First discovered in the early 1970s, Acanthamoeba keratitis has remained a major eye infection and presents a significant threat to the public health, especially in developing countries. The aim is to present a timely review of our current understanding of the advances made in this field in a comprehensible manner and includes novel concepts and provides clear directions for immediate research priorities. METHOD: We undertook a search of bibliographic databases for peer-reviewed research literature and also summarized our published results in this field...
May 10, 2018: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29730068/biofilm-testing-of-microbiota-an-essential-step-during-corneal-scrap-examination-in-egyptian-acanthamoebic-keratitis-cases
#10
Marwa A Hasby Saad, Haidy S M Khalil
PURPOSE: To detect co-infections in the culture-proven acanthamoebic keratitis (AK) cases, and to test the capability of biofilm formation in the isolated microbiota. The clinical findings, habit of wearing contact lens and in-vitro antibiotic resistance were analyzed further according to the biofilm formation capability. METHODS: After clinical examination, corneal scraps and swabs were taken from 240 clinically suspected AK cases, for Acanthamoeba and microbiological cultures...
May 3, 2018: Parasitology International
https://www.readbyqxmd.com/read/29723885/-early-diagnosis-and-successful-medical-treatment-of-acanthamoeba-keratitis
#11
Andrea Gyenes, Erika Orosz, Gábor László Sándor, Fabian Norbert Fries, Berthold Seitz, Nagy Zsolt Zoltán, Nóra Szentmáry
No abstract text is available yet for this article.
May 3, 2018: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/29698816/common-staining-techniques-for-highlighting-acanthamoeba-cysts
#12
Y Le Govic, A Gonçalves, L de Gentile, M Pihet
No abstract text is available yet for this article.
April 23, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29697492/acanthamoeba-an-overview-of-the-challenges-to-the-development-of-a-consensus-methodology-of-disinfection-efficacy-testing-for-contact-lens-care-products
#13
Jeffrey Brocious, Michelle E Tarver, Denise Hampton, Malvina Eydelman
With the increasing incidence of more pathogens that can cause microbial keratitis (MK), it is necessary to periodically reassess disinfection multipurpose solutions testing requirements to ensure that relevant organisms to challenge them are being used. Current testing protocols have included common pathogens such as Pseudomonas aeruginosa, Staphylococcus aureus, Serratia marcescens, Candida albicans, and Fusarium solani but have omitted less common pathogens such as Acanthamoeba. Specifically, Acanthamoeba sp...
April 24, 2018: Eye & Contact Lens
https://www.readbyqxmd.com/read/29688957/tap-water-and-risk-of-acanthamoeba-keratitis-in-rigid-gas-permeable-lens-wearers-sacred-cow-or-culprit-erratum
#14
(no author information available yet)
No abstract text is available yet for this article.
May 2018: Eye & Contact Lens
https://www.readbyqxmd.com/read/29682408/stability-of-an-ophthalmic-formulation-of-polyhexamethylene-biguanide-in-gamma-sterilized-and-ethylene-oxide-sterilized-low-density-polyethylene-multidose-eyedroppers
#15
Yassine Bouattour, Philip Chennell, Mathieu Wasiak, Mireille Jouannet, Valérie Sautou
Background: Polyhexamethylene biguanide (PHMB) eye drops are a frequently used medication to treat Acanthamoeba keratitis. In the absence of marketed PHMB eye drops, pharmacy-compounding units are needed to prepare this much needed treatment, but the lack of validated PHMB stability data severely limits their conservation by imposing short expiration dates after preparation. In this study we aim to assess the physicochemical and microbiological stability of a 0.2 mg/mL PHMB eye drop formulation stored in two kinds of polyethylene bottles at two different temperatures...
2018: PeerJ
https://www.readbyqxmd.com/read/29663139/corneal-biopsy-for-diagnosis-of-recalcitrant-microbial-keratitis
#16
Dana Robaei, U-Teng Chan, Pauline Khoo, Svetlana Cherepanoff, Yi-Chiao Li, Jane Hanrahan, Stephanie Watson
PURPOSE: To document the findings of corneal biopsies for progressive microbial keratitis in a large tertiary referral institution. METHODS: A retrospective medical records review of all patients who underwent at least one corneal biopsy for the diagnosis of microbial keratitis at Sydney Eye Hospital, Australia between January 1, 2010 and December 31, 2016 was performed. RESULTS: Thirty-eight patients (18 men and 20 women) underwent a corneal biopsy for progressive microbial keratitis unresponsive to broad-spectrum topical antimicrobials...
April 16, 2018: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/29657977/expert-practice-patterns-and-opinions-on-corneal-cross-linking-for-infectious-keratitis
#17
Yen C Hsia, Caitlin A Moe, Thomas M Lietman, Jeremy D Keenan, Jennifer Rose-Nussbaumer
Objective: To assess the current opinion and practice patterns from cornea experts regarding the benefit of corneal cross-linking (CXL) for infectious keratitis (IK). Methods and analysis: An international survey was distributed to corneal specialists via an internet survey. The survey data collected were analysed with descriptive statistics. Results: A survey was sent to 190 recipients, and 29 (15%) respondents completed the survey with an average of 7 years' experience using CXL...
2018: BMJ open ophthalmology
https://www.readbyqxmd.com/read/29642830/-a-new-strategy-for-enhancing-acanthamoebicidal-activity-with-synthesis-of-nanoflower-of-laurocerausus-officinalis-roemer-cherry-laurel-fruit-extracts
#18
Ayşe Baldemir, Ülkü Karaman, Sadi Yusufbeyoğlu, Ayşe Eken, Nilay Ildız, Selen İlgün, Cemil Çolak, Gamze Kaçmaz, İsmail Öçsoy, Soner Çankaya
Pathogenic Acanthamoeba species often cause infection known as Acanthamoeba keratitis among people who use contact lenses. It is a type of infection that can result in corneal ulceration, visual loss or even blindness, if not treated. There are various therapeutic options available in the treatment of Acanthamoeba infections but they are usually tough treatments with limited efficacy. For instance, hydrogen peroxide (H2 O2 ) is a commonly used contact lens disinfectant which is effective against Acanthamoeba but it is toxic to the cornea...
January 2018: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29625097/chloroquine-as-a-possible-disinfection-adjunct-of-disinfection-solutions-against-acanthamoeba
#19
Eun-Kyung Moon, Seungeun Lee, Fu-Shi Quan, Hyun-Hee Kong
Acanthamoeba keratitis is commonly encountered by contact lens wearers. Contact lens solution plays an important role in the safe use of contact lenses. The most popular products for disinfecting lenses are multipurpose disinfecting solutions (MPDS). However, almost all MPDS retailed in Korea are ineffective in killing Acanthamoeba. The objective of this study was to determine the possibility of using autophagy inhibitor chloroquine as a disinfecting agent to improve the amoebicidal activity of MPDS against Acanthamoeba, especially the cyst...
May 2018: Experimental Parasitology
https://www.readbyqxmd.com/read/29596605/evaluation-of-voriconazole-anti-acanthamoeba-polyphaga-in-vitro-activity-rat-cornea-penetration-and-efficacy-against-experimental-rat-acanthamoeba-keratitis
#20
Julie Gueudry, Laetitia Le Goff, Patricia Compagnon, Sabine Lefevre, Elodie Colasse, Camille Aknine, François Duval, Arnaud François, Romy Razakandrainibe, Jean Jacques Ballet, Marc Muraine, Loïc Favennec
Background: Acanthamoeba keratitis (AK) is a sight-threatening infectious disease. Its effective and safe medical therapy remains highly debated. Recently, voriconazole, a monotriazole with noted in vitro activity against a large variety of fungi, has been successfully used both topically and systemically to treat human AK cases. Objectives: To measure anti-Acanthamoeba polyphaga in vitro activity, anti-rat AK efficiency and rat cornea penetration of eye-drop and oral voriconazole...
March 27, 2018: Journal of Antimicrobial Chemotherapy
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