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Acanthamoeba keratitis

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https://www.readbyqxmd.com/read/29233457/antimicrobial-efficacy-of-a-novel-povidone-iodine-contact-lens-disinfection-system
#1
Katsuhide Yamasaki, Fumio Saito, Ritsue Ota, Simon Kilvington
PURPOSE: Contact lens (CL) wear is a risk factor for the acquisition of microbial keratitis. Accordingly, compliance to manufacturers' recommended hygiene and disinfection procedures are vital to safe (CL) use. In this study we evaluated a novel povidone-iodine (PI) (CL) disinfection system (cleadew, Ophtecs Corporation, Japan) against a range of bacterial, fungal and Acanthamoeba. METHODS: Antimicrobial assays were conducted according to ISO 14729 using the recommended strains of bacteria and fungi, with and without the presence of organic soil...
December 7, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/29221710/acanthamoeba-keratitis-in-patients-wearing-scleral-contact-lenses
#2
Matheus Porto Sticca, Linda C Carrijo-Carvalho, Isa M B Silva, Luiz A Vieira, Luciene B Souza, Rubens Belfort Junior, Fábio Ramos S Carvalho, Denise Freitas
PURPOSE: To report a series of cases of Acanthamoeba keratitis (AK) in scleral lens wearers with keratoconus to determine whether this type of contact lens presents a greater risk for development of infection. METHODS: This study reports three patients who wore scleral contact lenses to correct keratoconus and developed AK. The diagnoses of AK were established based on cultures of the cornea, scleral contact lenses, and contact lens paraphernalia. This study investigated the risk factors for infections...
December 5, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/29219900/diagnostic-challenges-in-nocardia-keratitis
#3
Joséphine Behaegel, Sorcha Ní Dhubhghaill, Carina Koppen
OBJECTIVE: To present a case of diagnostic confusion in Nocardia keratitis in a contact lens wearer and to illustrate the characteristic clinical findings of this rare entity. METHODS: Case report of Nocardia keratitis that was mistaken for acanthamoeba, herpetic, and fungal keratitis in three tertiary corneal referral centers before the correct diagnosis of Nocardia infection was made. RESULTS: A 29-year-old contact lens wearer was referred to our hospital for a contact lens-associated bacterial keratitis, not improving under standard bacterial treatment...
December 5, 2017: Eye & Contact Lens
https://www.readbyqxmd.com/read/29211242/potentially-pathogenic-acanthamoeba-genotype-t4-isolated-from-dental-units-and-emergency-combination-showers
#4
Esteban Castro-Artavia, Lissette Retana-Moreira, Jacob Lorenzo-Morales, Elizabeth Abrahams-Sandí
BACKGROUND: Acanthamoeba is the genus of free-living amoebae that is most frequently isolated in nature. To date, 20 Acanthamoeba genotypes have been described. Genotype T4 is responsible for approximately 90% of encephalitis and keratitis cases. Due to the ubiquitous presence of amoebae, isolation from environmental sources is not uncommon; to determine the clinical importance of an isolation, it is necessary to have evidence of the pathogenic potential of amoebae. OBJECTIVE: The aim of this study was to physiologically characterise 8 Acanthamoeba T4 isolates obtained from dental units and emergency combination showers and to determine their pathogenic potential by employing different laboratory techniques...
December 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/29203378/occurrence-of-acanthamoeba-genotypes-in-central-west-malaysian-environments
#5
Mohamad Hafiz Abdul Basher, Init Ithoi, Rohela Mahmud, Awatif Mohamed Abdulsalam, Agus Iwan Foead, Salwa Dawaki, Wahib Mohammed Mohsen Atroosh, Veeranoot Nissapatorn, Wan Omar Abdullah
Acanthamoeba species are ubiquitous free-living protozoa that can be found worldwide. Occasionally, it can become parasitic and the causative agent of acanthamoebic keratitis (AK) and Granulomatous Amoebic Encephalitis (GAE) in man. A total of 160 environmental samples and 225 naturally-infected animal corneal swabs were collected for Acanthamoeba cultivation. Acanthamoeba was found to be high in samples collected from environments (85%, 136/160) compared to infected animal corneas (24.89%, 56/225) by microscopic examination...
December 1, 2017: Acta Tropica
https://www.readbyqxmd.com/read/29133630/non-contact-lens-related-acanthamoeba-keratitis
#6
REVIEW
Prashant Garg, Paavan Kalra, Joveeta Joseph
The purpose of the study is to describe epidemiology, clinical features, diagnosis, and treatment of Acanthamoeba keratitis (AK) with special focus on the disease in nonusers of contact lenses (CLs). This study was a perspective based on authors' experience and review of published literature. AK accounts for 2% of microbiology-proven cases of keratitis. Trauma and exposure to contaminated water are the main predisposing factors for the disease. Association with CLs is seen only in small fraction of cases. Contrary to classical description experience in India suggests that out of proportion pain, ring infiltrate, and radial keratoneuritis are seen in less than a third of cases...
November 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29111996/microbial-keratitis-after-accelerated-corneal-collagen-cross-linking-in-keratoconus
#7
Prafulla K Maharana, Pranita Sahay, M Sujeeth, Deepali Singhal, Anubha Rathi, Jeewan S Titiyal, Namrata Sharma
PURPOSE: To assess the pattern of microbial keratitis after accelerated corneal collagen cross-linkage (aCXL) in patients with keratoconus. METHOD: The medical records of cases of keratoconus that underwent aCXL from June 2014 to May 2017 were reviewed. Cases that developed microbial keratitis after aCXL were included in the study. The clinical, microbiological profile and the treatment outcomes were evaluated. RESULTS: Of 532 eyes that underwent aCXL, 7 cases developed microbial keratitis during the study period...
November 2, 2017: Cornea
https://www.readbyqxmd.com/read/29111995/diagnostic-evaluation-of-co-occurrence-of-acanthamoeba-and-fungi-in-keratitis-a-preliminary-report
#8
Anita Raghavan, Shaffie Baidwal, Prabhu Vijayaraghavan, Sakthi Rajeswari, Revathi Rajaraman, Narendran Venkatapathy, Sunitha Menon, Ram Rammohan
PURPOSE: To test the hypothesis that the coexistence of Acanthamoeba with other forms of microbial keratitis, especially fungal keratitis (FK), is more prevalent than suspected. METHODS: A prospective diagnostic study whereby patients presenting with stromal keratitis were additionally tested for Acanthamoeba, irrespective of the initial diagnosis. In addition to the routine workup with Gram stain, KOH mount, and cultures on blood agar and potato dextrose agar, nonnutrient agar was included...
November 14, 2017: Cornea
https://www.readbyqxmd.com/read/29103900/chemical-composition-and-anti-acanthamoeba-activity-of-melaleuca-styphelioides-essential-oil
#9
Ferdaous Albouchi, Ines Sifaoui, Maria Reyes-Batlle, Atteneri López-Arencibia, José E Piñero, Jacob Lorenzo-Morales, Manef Abderrabba
Acanthamoeba infections cause serious humans diseases, such as amoebic keratitis and granulomatous amoebic encephalitis. Melaleuca essential oil has been reported to be effective in treating bacterial and fungal infections. However, the anti-parasitic effects of this essential oil are not well studied. In the present study, we first characterized the composition of the essential oil, extracted from the Tunisian Melaleuca styphelioides leaves, and then tested its effect on the Acanthamoeba castellanii Neff. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that the major common compounds were Caryophyllene oxide (23...
November 9, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/29102681/in%C3%A2-vitro-amoebicidal-and-antioxidant-activities-of-some-tunisian-seaweeds
#10
Olfa Chiboub, Leila Ktari, Ines Sifaoui, Atteneri López-Arencibia, Maria Reyes-Batlle, Mondher Mejri, Basilio Valladares, Manef Abderrabba, José E Piñero, Jacob Lorenzo-Morales
Free-living amoebae of genus Acanthamoeba are opportunistic pathogens widely distributed in the environment, and are the causative agents of several humans' infections, such as Acanthamoeba keratitis, Granulomatous Amoebic Encephalitis and also disseminated infections. The existence of the cyst stage complicates Acanthamoeba therapy as it is highly resistant to antibiotics and physical agents. All these facts reinforced the necessity to find and develop an effective therapy against Acanthamoeba infections. In the present study, we are interested to several seaweeds species collected from the Tunisian coasts and belonging to the 3 phyla (brown, green and red algae)...
November 2, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/29102680/ammoides-pusilla-apiaceae-essential-oil-activity-against-acanthamoeba-castellanii-neff
#11
Najet Souhaiel, Ines Sifaoui, Dorsaf Ben Hassine, Jean Bleton, Myriam Bonose, Fathi Moussa, José E Piñero, Jacob Lorenzo-Morales, Manef Abderrabba
Acanthamoeba is a free-living amoeba genus that causes several diseases namely, amoebic keratitis which is a painful sight threatening eyes disease. Its treatment is difficult and the exploration for new drugs is very important. The main objective of the present study was to evaluate the chemical composition of the Essential Oils (EO) obtained from leaves and flowers and aerial parts of Ammoides pusilla by an alternative method "Hydrodistillation''. Identification and quantification were realized by Gas Chromatography-Mass Spectrometry (GC-MS) and Gas Chromatography with Flame Ionization Detection (GC-FID)...
November 2, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/29097064/acanthamoeba-t4-trophozoites-cross-the-mdck-epithelium-without-cell-damage-but-increase-paracellular-permeability-and-transepithelial-resistance-by-modifying-tight-junction-composition
#12
Catalina Flores-Maldonado, Arturo González-Robles, Lizbeth Salazar-Villatoro, Maritza Omaña-Molina, Juan Manuel Gallardo, Mónica González-Lázaro, Verónica Ivonne Hernández-Ramírez, Patricia Talamás-Rohana, Jacob Lorenzo-Morales, Adolfo Martínez-Palomo
Free-living amoebae of the genus Acanthamoeba are protozoa ubiquitously found in nature. Some species of the genus are potentially pathogenic for humans provoking keratitis in healthy individuals, often in contact lens wearers and opportunistic infections such as pneumonitis, fatal granulomatous encephalitis and skin infections, particularly in immunocompromised individuals. The pathogenic mechanisms of these amoebae are poorly understood, however it had been suggested that contact dependent mechanisms are important during invasion, regardless of the epithelia type, since amoebae penetrate epithelia separating tight junction (TJ)...
October 31, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/29080613/phylogeography-genetic-variability-and-structure-of-acanthamoeba-metapopulations-in-iran-inferred-by-18s-ribosomal-rna-sequences-a-systematic-review-and-meta-analysis
#13
REVIEW
Adel Spotin, Hamid Reza Moslemzadeh, Mahmoud Mahami-Oskouei, Ehsan Ahmadpour, Maryam Niyyati, Seyed Hossein Hejazi, Fatemeh Memari, Jafar Noori
OBJECTIVE: To verify phylogeography and genetic structure of Acanthamoeba populations among the Iranian clinical isolates and natural/artificial environments distributed in various regions of the country. METHODS: We searched electronic databases including Medline, PubMed, Science Direct, Scopus and Google Scholar from 2005 to 2016. To explore the genetic variability of Acanthamoeba sp, 205 sequences were retrieved from keratitis patients, immunosuppressed cases and environmental sources as of various geographies of Iran...
September 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29033270/a-survey-of-contact-lens-related-complications-in-a-tertiary-hospital-in-china
#14
Weiwei Li, Xuguang Sun, Zhiqun Wang, Yang Zhang
PURPOSE: To describe the type of complications related to contact lens (CL) wear in a tertiary hospital in XX, China. METHODS: A retrospective study of 141 patients who complained discomfort after wearing contact lens on an outpatient basis of XX Hospital were conducted from the January 2012 to December 2015. The data included patients' demographics, lens type, history, slit-lamp examination, reports of corneal scrapings, culture, and examination of in vivo confocal microscopy...
October 12, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/28974450/acanthamoeba-culbertsoni-isolated-from-a-clinical-case-with-intraocular-dissemination-structure-and-in%C3%A2-vitro-analysis-of-the-interaction-with-hamster-cornea-and-mdck-epithelial-cell-monolayers
#15
Arturo González-Robles, Maritza Omaña-Molina, Lizbeth Salazar-Villatoro, Catalina Flores-Maldonado, Jacob Lorenzo-Morales, María Reyes-Batlle, Francisco Arnalich-Montiel, Adolfo Martínez-Palomo
Acanthamoeba culbertsoni trophozoites, previously isolated from a human keratitis case with severe intraocular damage, were maintained in axenic culture. Co-incubation of amoebae with MDCK cell monolayers demonstrated an apparent preference of the amoebae to introduce themselves between the cells. The trophozoites appeared to cross the cell monolayer through the tight junctions, which resulted in decreased trans-epithelial resistance (TER) measurements. Unexpectedly, after co-incubation of amoebae with hamster corneas, we observed that the trophozoites were able to cross the different cell layers and reach the corneal stroma after only 12 h of interaction, in contrast to other Acanthamoeba species...
September 30, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28969610/associated-factors-diagnosis-and-management-of-acanthamoeba-keratitis-in-a-referral-center-in-southern-china
#16
Jing Zhong, Xingyi Li, Yuqing Deng, Ling Chen, Shiyou Zhou, Weilan Huang, Shiqi Lin, Jin Yuan
BACKGROUND: To analyse the associated factors, diagnosis, clinical manifestations and therapeutic effects of Acanthamoeba keratitis at a tertiary ophthalmic centre in Southern China. METHODS: A retrospective clinical study was performed in fifteen patients who were admitted to Zhongshan Ophthalmic Centre (ZOC) from January 2004 to December 2014. The patients' pathogenesis-associated factors were analysed, and preoperative diagnoses were determined using corneal scraping cultures and/or confocal microscopy followed...
October 2, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28965795/acanthamoeba-keratitis-in-noncompliant-soft-contact-lenses-users-genotyping-and-risk-factors-a-study-from-cairo-egypt
#17
Eman E Taher, Eman M H Méabed, Islam Abdallah, Wafaa Y Abdel Wahed
Acanthamoeba keratitis (AK) is a severe corneal infection that may occur as a serious outcome of improper use of contact lenses (CL). OBJECTIVES: The study aimed to diagnose AK in soft CL users presenting with infectious keratitis, and to identify the prevalent genotypes isolated from different cases. Another aim was to determine the CL hygiene-related risk behaviors, and to explore the risk of water exposure for developing AK. METHODS: A cross sectional study was performed...
September 28, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28965660/assessment-of-confocal-microscopy-for-the-diagnosis-of-polymerase-chain-reaction-positive-acanthamoeba-keratitis-a-case-control-study
#18
Sophie De Craene, Juliette Knoeri, Cristina Georgeon, Philippe Kestelyn, Vincent M Borderie
PURPOSE: To determine in vivo confocal microscopy diagnostic criteria to diagnose Acanthamoeba keratitis (AK) using polymerase chain reaction (PCR) as the reference diagnostic technique. DESIGN: Retrospective case-control study. Data were recorded prospectively and analyzed retrospectively. PARTICIPANTS: Fifty patients with PCR-positive AK (study group) and 50 patients with bacterial, fungal, viral, or immune keratitis featuring negative Acanthamoeba PCR results (control group)...
September 28, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28964757/risk-factors-and-clinical-features-of-acanthamoeba-keratitis-in-a-tunisian-tertiary-eye-care-center
#19
Mejdi Boukari, Khalil Erraïs, Imen Ammous, Khaoula El Amri-Mezghanni, Imen Zhioua-Braham, Meriam Bouchekoua, Sonia Trabelsi, Dorsaf Aloui, Samira Khaled, Mabrouka Saidani, Sonia Mabrouk, Ilhem Mili-Boussen, Raja Zhioua
No abstract text is available yet for this article.
September 28, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28954958/role-of-fatty-acid-salts-as-anti-acanthamoeba-agents-for-disinfecting-contact-lens
#20
Aya Tanaka, Mariko Era, Yumeho Obata, Manami Masuda, Takayoshi Kawahara, Takahide Kanyama, Hiroshi Morita
 Acanthamoeba is found in seawater, fresh water, and soil and is an opportunistic pathogen that causes a potentially blinding corneal infection known as Acanthamoeba keratitis. The anti-amoeba activity of 9 fatty acid salts (potassium butyrate (C4K), caproate (C6K), caprylate (C8K), caprate (C10K), laurate (C12K), myristate (C14K), oleate (C18:1K), linoleate (C18:2K), and linolenate (C18:3K)) was tested on Acanthamoeba castellanii ATCC 30010 (trophozoites and cysts). Fatty acid salts (350 mM and pH 10.5) were prepared by mixing fatty acids with the appropriate amount of KOH...
2017: Biocontrol Science
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