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https://www.readbyqxmd.com/read/29327237/regional-age-and-respiratory-secretion-specific-prevalence-of-respiratory-viruses-associated-with-asthma-exacerbation-a-literature-review
#1
REVIEW
Xue-Yan Zheng, Yan-Jun Xu, Wei-Jie Guan, Li-Feng Lin
Despite increased understanding of how viral infection is involved in asthma exacerbations, it is less clear which viruses are involved and to what extent they contribute to asthma exacerbations. Here, we sought to determine the prevalence of different respiratory viruses during asthma exacerbations. Systematic computerized searches of the literature up to June 2017 without language limitation were performed. The primary focus was on the prevalence of respiratory viruses, including AdV (adenovirus), BoV (bocavirus), CoV (coronavirus), CMV (cytomegalovirus), EnV (enterovirus), HSV (herpes simplex virus), IfV (influenza virus), MpV (metapneumovirus), PiV (parainfluenzavirus), RV (rhinovirus) and RSV (respiratory syncytial virus) during asthma exacerbations...
January 11, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29305503/elucidating-waterborne-pathogen-presence-and-aiding-source-apportionment-in-an-impaired-stream
#2
Jennifer Weidhaas, Angela Anderson, Rubayat Jamal
Fecal indicator bacteria (FIB) are the basis for water quality regulations and are considered proxies for waterborne pathogens when conducting human health risk assessments. Direct detection of pathogens in water and simultaneous identification of the source of fecal contamination is possible with microarrays, circumventing drawbacks to FIB approaches. A multi-gene target microarray was used to assess the prevalence of waterborne pathogens in a fecally impaired, mixed use watershed. Results indicate that fecal coliforms have improved substantially in the watershed since its listing as a 303(d) impaired stream in 2002 and are now near United States recreational water criteria standards...
January 5, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29299861/human-bocavirus-detection-quantification-and-molecular-characterization-in-sewage-and-surface-waters-in-uruguay
#3
Matías Salvo, Andrés Lizasoain, Matías Castells, Viviana Bortagaray, Sebastián Castro, Rodney Colina, Fernando Lopez Tort, Matías Victoria
Human bocavirus (HBoV) infections are related to respiratory and gastroenteric diseases. The aim of this study was to investigate the presence of HBoV in both sewage and surface waters in Uruguay. Sixty-eight sewage samples from the cities of Salto, Paysandú, Bella Unión, Fray Bentos, Treinta y Tres and Melo and 36 surface water samples from the cities of Salto, Florida and Santa Lucía were studied. HBoV was screened by multiplex qPCR for the detection of the four subtypes, followed by monoplex qPCRs for the independent quantification of each subtype...
January 3, 2018: Food and Environmental Virology
https://www.readbyqxmd.com/read/29243138/first-molecular-detection-of-porcine-bocavirus-in-malaysia
#4
Daniel Mohan Jacob, Chee Yien Lee, Siti Suri Arshad, Gayathri Thevi Selvarajah, Faruku Bande, Bee Lee Ong, Peck Toung Ooi
Several strains of porcine bocaviruses have been reported worldwide since their first detection in Sweden in 2009. Subsequently, the virus has been reported to be associated with gastrointestinal and respiratory signs in weaner and grower pigs. Although Malaysia is host to a self-sufficient swine livestock industry, there is no study that describes porcine bocavirus in the country. This report is the first to describe porcine bocavirus (PBoV) in Malaysian swine herds. PBoV was identified in various tissues from sick and runt pigs using the conventional PCR method with primers targeting conserved regions encoding for the nonstructural protein (NS1) gene...
December 14, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29207326/in-silico-prediction-of-potential-vaccine-candidates-on-capsid-protein-of-human-bocavirus-1
#5
Narayanan Kalyanaraman
Human bocavirus 1 (HBoV1) is a newly identified parvovirus that causes serious respiratory infection among children across the globe. Aim of the present study was to predict immunogenic residues located on the VP2 protein of HBoV1 towards development of epitope based vaccines. Several computational tools were employed to predict epitopes (bothT and B cell restricted) with stringent regulation for the improvement of confidence. After meticulous analysis, the peptide "TTPWTYFNFNQY" was identified as potential candidate for development of preventive vaccine...
December 2, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29155041/epidemiological-and-clinical-characteristics-of-coronavirus-and-bocavirus-respiratory-infections-after-allogeneic-stem-cell-transplantation-a-prospective-single-center-study
#6
José Luis Piñana, Silvia Madrid, Ariadna Pérez, Juan Carlos Hernández-Boluda, Estela Giménez, María José Terol, Marisa Calabuig, David Navarro, Carlos Solano
Epidemiological data about coronaviruses (CoVs) and human bocavirus (HBoV) in the setting of allogeneic hematopoietic stem cell transplantation (allo-HSCT) is scarce. METHODS: We conducted a prospective longitudinal study on respiratory viral infections (RVIs) in allo-HSCT recipients having respiratory symptoms from December 2013 until June 2016. Respiratory virus in upper and/or lower respiratory tract (URT and LRT) specimens were tested using Luminex xTAG RVP Fast v1 assay. RESULTS: Seventy-nine consecutive allo-HSCT recipients developed a total of 192 virologically documented RVI episodes over 30 months...
November 15, 2017: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/29122866/genome-sequence-of-a-porcine-bocavirus-detected-in-feces-of-domestic-minks-in-china
#7
Yan Wang, Jing Zhao, Min Zheng, Zhijian Liu, Jiaqi Yuan, Jieji Zhao, Quan Shen, Zhaobin Fan, Lili Jiang, Shixing Yang
We report here the genome sequence of porcine bocavirus strain PBov-JZ08, which was isolated from mink feces in China. Sequence analysis implied that PBov-JZ08 clustered with three porcine bocaviruses.
November 9, 2017: Genome Announcements
https://www.readbyqxmd.com/read/29028159/serologically-diagnosed-acute-human-bocavirus-1-infection-in-childhood-community-acquired-pneumonia
#8
Amanda C Nascimento-Carvalho, Ana-Luisa Vilas-Boas, Maria-Socorro H Fontoura, Man Xu, Tytti Vuorinen, Maria Söderlund-Venermo, Olli Ruuskanen, Cristiana M Nascimento-Carvalho
AIM: To assess the role of human bocavirus 1 (HBoV1) as a causative agent of non-severe community-acquired pneumonia (CAP) in children. METHODS: Patients aged 2-59 months with non-severe CAP (respiratory complaints and radiographic pulmonary infiltrate/consolidation) attending a University Hospital in Salvador, Brazil were enrolled in a prospective cohort. From 820 recruited children in a clinical trial (ClinicalTrials.gov NCT01200706), nasopharyngeal aspirate (NPA), and acute and convalescent serum samples were obtained from 759 (92...
October 13, 2017: Pediatric Pulmonology
https://www.readbyqxmd.com/read/29021400/human-parvovirus-infection-of-human-airway-epithelia-induces-pyroptotic-cell-death-via-inhibiting-apoptosis
#9
Xuefeng Deng, Wei Zou, Min Xiong, Zekun Wang, John F Engelhardt, Shui Qing Ye, Ziying Yan, Jianming Qiu
Human bocavirus 1 (HBoV1) is a human parvovirus that causes acute respiratory tract infections in young children. In this study, we confirmed that, when polarized/well-differentiated human airway epithelia are infected with HBoV1 in vitro, they develop damage characterized by barrier function disruption and cell hypotrophy. Cell death mechanism analyses indicated that the infection induced pyroptotic cell death characterized by caspase-1 activation. Unlike infections with other parvoviruses, HBoV1 infection did not activate the apoptotic or necroptotic cell death pathway...
October 11, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28934425/human-bocavirus-capsid-messenger-rna-detection-in-children-with-pneumonia
#10
MULTICENTER STUDY
Robert Schlaberg, Krow Ampofo, Keith D Tardif, Chris Stockmann, Keith E Simmon, Weston Hymas, Steven Flygare, Brett Kennedy, Anne Blaschke, Karen Eilbeck, Mark Yandell, Jon A McCullers, Derek J Williams, Kathryn Edwards, Sandra R Arnold, Anna Bramley, Seema Jain, Andrew T Pavia
Background: The role of human bocavirus (HBoV) in respiratory illness is uncertain. HBoV genomic DNA is frequently detected in both ill and healthy children. We hypothesized that spliced viral capsid messenger RNA (mRNA) produced during active replication might be a better marker for acute infection. Methods: As part of the Etiology of Pneumonia in the Community (EPIC) study, children aged <18 years who were hospitalized with community-acquired pneumonia (CAP) and children asymptomatic at the time of elective outpatient surgery (controls) were enrolled...
September 15, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28924489/identification-of-an-enterovirus-recombinant-with-a-torovirus-like-gene-insertion-during-a-diarrhea-outbreak-in-fattening-pigs
#11
Nádia Conceição-Neto, Sebastiaan Theuns, Tingting Cui, Mark Zeller, Claude Kwe Yinda, Isaura Christiaens, Elisabeth Heylen, Marc Van Ranst, Sebastien Carpentier, Hans J Nauwynck, Jelle Matthijnssens
Diarrhea outbreaks in pig farms have raised major concerns in Europe and USA, as they can lead to dramatic pig losses. During a suspected outbreak in Belgium of porcine epidemic diarrhea virus (PEDV), we performed viral metagenomics to assess other potential viral pathogens. Although PEDV was detected, its low abundance indicated that other viruses were involved in the outbreak. Interestingly, a porcine bocavirus and several enteroviruses were most abundant in the sample. We also observed the presence of a porcine enterovirus genome with a gene insertion, resembling a C28 peptidase gene found in toroviruses, which was confirmed using re-sequencing, bioinformatics, and proteomics approaches...
July 2017: Virus Evolution
https://www.readbyqxmd.com/read/28922613/the-etiology-of-community-acquired-pneumonia-among-children-under-5%C3%A2-years-of-age-in-mainland-china-2001-2015-a-systematic-review
#12
Guijun Ning, Xuxia Wang, Dan Wu, Zundong Yin, Yixing Li, Huaqing Wang, Weizhong Yang
BACKGROUND: The aim of this systematic review was to examine the etiology of community-acquired pneumonia (CAP) among Chinese children younger than 5 y and provide evidence for further cost-effectiveness analyses for vaccine development, diagnostic strategies and empirical treatments. METHODS: The literature review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement. Data were obtained by searching PubMed, Embase, Web-of Science, and the Chinese databases Wanfang Data and China National Knowledge Infrastructure...
November 2, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28910409/risk-of-acute-gastroenteritis-associated-with-human-bocavirus-infection-in-children-a-systematic-review-and-meta-analysis
#13
REVIEW
Ri De, Liying Liu, Yuan Qian, Runan Zhu, Jie Deng, Fang Wang, Yu Sun, Huijin Dong, Liping Jia, Linqing Zhao
Human bocaviruses (HBoVs), which were first identified in 2005 and are composed of genotypes 1-4, have been increasingly detected worldwide in pediatric patients with acute gastroenteritis. To investigate if HBoV infection is a risk factor of acute gastroenteritis in children younger than 5 years old, we searched PubMed, Embase (via Ovid), the Chinese Biomedical Literature Database (CBM), and the Cochrane Library for studies assessing the prevalence of HBoVs in individuals from Oct 25, 2005 to Oct 31, 2016...
2017: PloS One
https://www.readbyqxmd.com/read/28883450/virome-analysis-for-identification-of-novel-mammalian-viruses-in-bats-from-southeast-china
#14
Dan Hu, Changqiang Zhu, Yi Wang, Lele Ai, Lu Yang, Fuqiang Ye, Chenxi Ding, Jiafeng Chen, Biao He, Jin Zhu, Hui Qian, Wenrong Xu, Youjun Feng, Weilong Tan, Changjun Wang
Bats have been shown as important mammal resevoirs to carry a variety of zoonotic pathogens. To analyze pathogenic species in bats from southeast coastal regions of China, we performed metagenomic sequencing technology for high throughput sequencing of six sentinels from southeast coastal area of China. We obtained 5,990,261 high quality reads from intestine and lung tissue of 235 bats, including 2,975,371 assembled sequences. 631,490 reads predicted overlapping sequences for the open reading frame (ORF), which accounts for 2...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28876460/viral-etiology-of-sporadic-cases-of-parotitis-among-children-in-korea-during-2013-2014
#15
Hae J Kang, Sun H Kim, Jae K Chung, Soon W Lee, Seung B Choi, Hye E Eom, Ok Park, Kisoon Kim, Sung S Kim
Recent years have seen a high incidence of mumps, which is generally diagnosed based on clinical features, especially parotitis, without laboratory confirmation in Korea. To better understand the epidemiology of mumps in Korean children, we investigated sporadic suspected mumps cases with parotitis. In total, 237 buccal swabs or throat swabs collected from children with parotitis who had been clinically diagnosed with mumps were tested using real-time PCR for the detection of six viruses (Epstein-Barr virus, Human herpesvirus 6, Mumps virus, Human parainfluenza virus-1, -2, -3, Human adenovirus, Human bocavirus)...
September 6, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28854225/enteric-viruses-in-hiv-1-seropositive-and-hiv-1-seronegative-children-with-diarrheal-diseases-in-brazil
#16
Silvana Augusta Rodrigues Portes, Filipe Anibal Carvalho-Costa, Monica Simões Rocha, Tulio Machado Fumian, Adriana Gonçalves Maranhão, Rosane Maria de Assis, Maria da Penha Trindade Pinheiro Xavier, Myrna Santos Rocha, Marize Pereira Miagostovich, José Paulo Gagliardi Leite, Eduardo de Mello Volotão
Diarrheal diseases (DD) have distinct etiological profiles in immune-deficient and immune-competent patients. This study compares detection rates, genotype distribution and viral loads of different enteric viral agents in HIV-1 seropositive (n = 200) and HIV-1 seronegative (n = 125) children hospitalized with DD in Rio de Janeiro, Brazil. Except for group A rotavirus (RVA), which were detected through enzyme immunoassay, the other enteric viruses (norovirus [NoV], astrovirus [HAstV], adenovirus [HAdV] and bocavirus [HBoV]) were detected through PCR or RT-PCR...
2017: PloS One
https://www.readbyqxmd.com/read/28796554/adeno-associated-virus-human-bocavirus-1-chimeric-vectors-ferreting-out-their-role-in-airway-gene-therapy
#17
Terence R Flotte
No abstract text is available yet for this article.
August 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/28777951/human-bocavirus-1-infection-of-caco-2-cell-line-cultures
#18
Lucía María Ghietto, Ana Paola Toigo D'Angelo, Franco Agustin Viale, María Pilar Adamo
Human bocavirus 1 (HBoV1) is a parvovirus associated with pneumonia in infants. It has been detected in different tissues, including colorectal tumors. In this study, we investigated whether Caco-2 cell line, derived from human colon cancer, can be utilized as a model for HBoV1 replication. We demonstrate HBoV1 replication in Caco-2 cultures supplemented with DEAE-dextran after inoculation with respiratory material from infected patients presenting with acute respiratory infection. A viral cycle of rapid development is displayed...
October 2017: Virology
https://www.readbyqxmd.com/read/28763071/prevalence-of-respiratory-viruses-in-wheezing-children-not-older-than-24-months-of-age
#19
Martín Bedolla-Barajas, Hilda Montero, Jaime Morales-Romero, Adriana Landa-Cardeña, Jahaziel Díaz, Netzahualpilli Delgado-Figueroa, Luis Gustavo Orozco-Alatorre
INTRODUCTION: Wheezing in children not older than 24 months of age is a frequent event, and viruses are usually the causative agents. The aim of the study was to estimate the prevalence of respiratory viruses in wheezing children who were not older than 24 months of age and who had no history of asthma. METHODS: Fifty-five Mexican children were included in an analytical cross-sectional study. Nasal secretions were obtained by using sterile rayon-tipped applicators to identify the virus by polymerase chain reaction or reverse transcription-polymerase chain reaction: adenovirus, bocavirus, human rhinovirus, influenza virus type A, human metapneumovirus, parainfluenza, rhinovirus, and respiratory syncytial virus...
May 2017: Gaceta Médica de México
https://www.readbyqxmd.com/read/28731923/human-bocavirus-can-it-trigger-hemophagocytic-lymphohistiocytosis
#20
Sevgen Tanir Basaranoglu, Kubra Aykac, Yasemin Ozsurekci, Inci Bajin, Betul Tavil, Fatma Gumruk, Mehmet Ceyhan
With improvements in molecular diagnostic methods, report of Human bocavirus (HBoV) as an etiologic agent in many studies on viral respiratory and gastrointestinal infections has been increasing. Two pediatric patients who presented with secondary hemophagocytic lymphohistiocytosis were examined for etiologic causes, including viruses. Whole bacterial and fungal cultures and viral serological studies were negative. Viral polymerase chain reaction of nasopharyngeal secretions showed HBoV. One was successfully treated with intravenous immunoglobulins, whereas the other died with multiorgan failure...
November 2017: Journal of Pediatric Hematology/oncology
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