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https://www.readbyqxmd.com/read/27907010/viral-metagenomic-analysis-displays-the-co-infection-situation-in-healthy-and-pmws-affected-pigs
#1
Anne-Lie Blomström, Caroline Fossum, Per Wallgren, Mikael Berg
The development of high-throughput sequencing technologies have allowed the possibility to investigate and characterise the entire microbiome of individuals, providing better insight to the complex interaction between different microorganisms. This will help to understand how the microbiome influence the susceptibility of secondary agents and development of disease. We have applied viral metagenomics to investigate the virome of lymph nodes from Swedish pigs suffering from the multifactorial disease postweaning multisystemic wasting syndrome (PMWS) as well as from healthy pigs...
2016: PloS One
https://www.readbyqxmd.com/read/27881651/alternative-polyadenylation-of-human-bocavirus-at-its-3-end-is-regulated-by-multiple-elements-and-affects-capsid-expression
#2
Sujuan Hao, Junmei Zhang, Zhen Chen, Huanzhou Xu, Hanzhong Wang, Wuxiang Guan
: Alternative processing of human bocavirus (HBoV) P5 promoter-transcribed RNA is critical for generating the structural and nonstructural protein-encoding mRNA transcripts. The regulatory mechanism by which HBoV RNA transcripts are polyadenylated at proximal [(pA)p] or distal [(pA)d] polyadenylation sites is yet unclear. We constructed a recombinant HBoV infectious clone to study the alternative polyadenylation regulation of HBoV. Surprisingly, in addition to the reported distal polyadenylation site (pA)d, a novel distal polyadenylation site (pA)d2 which is located in the right end hairpin (REH), was identified during infectious clone transfection or recombinant virus infection...
November 23, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27871815/identification-and-genomic-characterization-of-a-novel-rat-bocavirus-from-brown-rats-in-china
#3
Susanna K P Lau, Hazel C Yeung, Kenneth S M Li, Carol S F Lam, Jian-Piao Cai, Ming-Chi Yuen, Ming Wang, Bo-Jian Zheng, Patrick C Y Woo, Kwok-Yung Yuen
Despite recent discoveries of novel animal bocaparvoviruses, current understandings on the diversity and evolution of bocaparvoviruses are still limited. We report the identification and genome characterization of a novel bocaparvovirus, rat bocaparvovirus (RBoV), in brown rats (Rattus norvegicus) in China. RBoV was detected in 11.5%, 2.4%, 16.2% and 0.3% of alimentary, respiratory, spleen and kidney samples respectively, of 636 brown rats by PCR, but not in samples of other rodent species, suggesting that brown rats are the primary reservoir of RBoV...
November 19, 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27854115/an-investigation-into-respiratory-tract-viruses-in-children-with-acute-lower-respiratory-tract-infection-or-wheezing
#4
Demet Dabaniyasti, Fahriye Eksi, Özlem Keskin, Mehmet Y Özkars, Tekin Karsligil, Iclal Balci
BACKGROUND: This study aimed to determine the frequencies of respiratory tract viruses in patient (acute lower respiratory tract infection (LRTI) or wheezing) and control (history of asthma without symptoms) groups. METHODS: Using multiplex-PCR, respiratory tract viruses were investigated in the respiratory tract specimens from patient and control groups followed in the Pediatric Clinic. RESULTS: The viruses detected in the patient and control groups (p=0...
November 17, 2016: Minerva Pediatrica
https://www.readbyqxmd.com/read/27818586/human-bocavirus-current-knowledge-and-future-challenges
#5
REVIEW
Marcello Guido, Maria Rosaria Tumolo, Tiziano Verri, Alessandro Romano, Francesca Serio, Mattia De Giorgi, Antonella De Donno, Francesco Bagordo, Antonella Zizza
Human bocavirus (HBoV) is a parvovirus isolated about a decade ago and found worldwide in both respiratory samples, mainly from early life and children of 6-24 mo of age with acute respiratory infection, and in stool samples, from patients with gastroenteritis. Since then, other viruses related to the first HBoV isolate (HBoV1), namely HBoV2, HBoV3 and HBoV4, have been detected principally in human faeces. HBoVs are small non-enveloped single-stranded DNA viruses of about 5300 nucleotides, consisting of three open reading frames encoding the first two the non-structural protein 1 (NS1) and nuclear phosphoprotein (NP1) and the third the viral capsid proteins 1 and 2 (VP1 and VP2)...
October 21, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27806994/human-parvoviruses
#6
REVIEW
Jianming Qiu, Maria Söderlund-Venermo, Neal S Young
Parvovirus B19 (B19V) and human bocavirus 1 (HBoV1), members of the large Parvoviridae family, are human pathogens responsible for a variety of diseases. For B19V in particular, host features determine disease manifestations. These viruses are prevalent worldwide and are culturable in vitro, and serological and molecular assays are available but require careful interpretation of results. Additional human parvoviruses, including HBoV2 to -4, human parvovirus 4 (PARV4), and human bufavirus (BuV) are also reviewed...
January 2017: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/27800148/prevalence-and-seasonal-distribution-of-respiratory-viruses-during-the-2014-2015-season-in-istanbul
#7
Safak Goktas, Mumtaz Cem Sirin
BACKGROUND: Acute respiratory tract infection (ARTI) is one of the most common infections worldwide, causing significant morbidity and mortality. OBJECTIVES: This study was conducted to determine the prevalence and seasonal distribution of respiratory viruses in our region, in children and adults with a pre-diagnosis of ARTI. METHODS: A total of 845 nasopharyngeal swab specimens were analyzed with the RespiFinder Smart 22 kit (PathoFinder BV, Netherlands) and the Rotor-Gene 6000 real-time PCR system...
September 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27774707/bocavirus-infection-following-paediatric-liver-transplantation
#8
M Y Tan, L N Tan, M M Aw, S H Quak, S V Karthik
HBoV is an emergent virus, which is frequently detected as a co-infective agent. However, it can cause disease on its own. It is associated with respiratory and diarrhoeal illness in children and adults, whether immunocompetent or immunocompromised. We report HBoV infection in a child post-liver transplantation, who presented with persistent fever and mild tachypnea, 3 weeks after a successful transplant. She recovered spontaneously with no graft dysfunction.
October 23, 2016: Pediatric Transplantation
https://www.readbyqxmd.com/read/27735999/multiplex-rt-pcr-for-rapid-detection-of-viruses-commonly-causing-diarrhea-in-pediatric-patients
#9
Aksara Thongprachum, Pattara Khamrin, Ngan Thi Kim Pham, Sayaka Takanashi, Shoko Okitsu, Hiroyuki Shimizu, Niwat Maneekarn, Satoshi Hayakawa, Hiroshi Ushijima
Multiplex RT-PCR method using five sets of panel primers was developed for the detection of diarrheal viruses, including rotavirus A, B, and C, adenovirus, astrovirus, norovirus GI and GII, sapovirus, Aichi virus, parechovirus, enterovirus, cosavirus, bocavirus, and Saffold virus. The sensitivity of the method was evaluated and tested with 751 fecal specimens collected from Japanese children with acute diarrhea. Several kinds of viruses were detected in 528 out of 751 (70.3%) fecal specimens. Mixed-infection with different viruses in clinical specimens could also be effectively detected...
October 13, 2016: Journal of Medical Virology
https://www.readbyqxmd.com/read/27733644/the-dna-damage-signaling-is-required-for-replication-of-the-hbov1-dna-in-dividing-hek293-cells
#10
Xuefeng Deng, Peng Xu, Wei Zou, Weiran Shen, Jianxin Peng, Kaiyu Liu, John F Engelhardt, Ziying Yan, Jianming Qiu
: Human bocavirus 1 (HBoV1), an emerging human pathogenic respiratory virus, is a member of the genus Bocaparvovirus of the Parvoviridae family. In human airway epithelia air-liquid interface (HAE-ALI) cultures, HBoV1 infection initiates a DNA damage response (DDR), activating all three phosphatidylinositol 3-kinase-related kinases (PI3KKs): ATM, ATR and DNA-PKcs. In this context, activation of PI3KKs is a requirement for amplification of the HBoV1 genome (PLoS Pathog., 2016; 12:e1005399), and HBoV1 replicates only in terminally differentiated, non-dividing cells...
October 12, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27723525/a-four-year-seasonal-survey-of-the-relationship-between-outdoor-climate-and-epidemiology-of-viral-respiratory-tract-infections-in-a-temperate-climate
#11
Nicklas Sundell, Lars-Magnus Andersson, Robin Brittain-Long, Magnus Lindh, Johan Westin
BACKGROUND: The relation between weather conditions, viral transmission and seasonal activity of respiratory viruses is not fully understood. OBJECTIVES: To investigate the impact of outdoor weather in a temperate climate setting on the seasonal epidemiology of viruses causing respiratory tract infections, particularly influenza A (IFA). STUDY DESIGN: In total, 20,062 clinical nasopharyngeal swab samples referred for detection of respiratory pathogens using a multiplex PCR panel, between October 2010 and July 2013, were included...
October 7, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27714467/is-the-role-of-rhinoviruses-as-causative-agents-of-pediatric-community-acquired-pneumonia-over-estimated
#12
Tatyana V Spichak, Svetlana B Yatsyshina, Lubov К Кatosova, Svetlana S Кim, Matti O Korppi
: The role that rhinoviruses, enteroviruses, parainfluenza viruses, coronaviruses and human bocavirus play in pediatric pneumonia is insufficiently studied. We used polymerase chain reaction (PCR) to study 9 virus groups, including 16 different viruses or viral strains, in 56 ambulatory children with radiologically confirmed community-acquired pneumonia (CAP). The same tests were carried out on 474 apparently healthy control children of the same age and sex. The mean age of children with CAP was 6...
October 6, 2016: European Journal of Pediatrics
https://www.readbyqxmd.com/read/27667752/respiratory-viruses-and-influenza-like-illness-epidemiology-and-outcomes-in-children-aged-6-months-to-10-years-in-a%C3%A2-multi-country-population-sample
#13
Sylvia Taylor, Pio Lopez, Lily Weckx, Charissa Borja-Tabora, Rolando Ulloa-Gutierrez, Eduardo Lazcano-Ponce, Angkool Kerdpanich, Miguel Angel Rodriguez Weber, Abiel Mascareñas de Los Santos, Juan-Carlos Tinoco, Marco Aurelio P Safadi, Fong Seng Lim, Marcela Hernandez-de Mezerville, Idis Faingezicht, Aurelio Cruz-Valdez, Yang Feng, Ping Li, Serge Durviaux, Gerco Haars, Sumita Roy-Ghanta, David W Vaughn, Terry Nolan
BACKGROUND: Better population data on respiratory viruses in children in tropical and southern hemisphere countries is needed. METHODS: The epidemiology of respiratory viruses among healthy children (6 months to <10 years) with influenza-like illness (ILI) was determined in a population sample derived from an influenza vaccine trial (NCT01051661) in 17 centers in eight countries (Australia, South East Asia and Latin America). Active surveillance for ILI was conducted for approximately 1 year (between February 2010 and August 2011), with PCR analysis of nasal and throat swabs...
September 22, 2016: Journal of Infection
https://www.readbyqxmd.com/read/27664943/detection-of-poliovirus-infection-in-children-with-acute-gastroenteritis-in-chiang-mai-thailand
#14
Kattareeya Kumthip, Pattara Khamrin, Niwat Maneekarn
Poliovirus (PV) is typically transmitted by the fecal-oral route, which means that the risk of infection and virus distribution could be achieved by exposure to the virus contaminated in food and water. The aim of this study was to determine the occurrence of PV strains by detecting the virus in pediatric patients who admitted to the hospitals with diarrhea in Chiang Mai, Thailand during 2010-2015. By applying a reverse transcription-polymerase chain reaction and nucleotide sequencing analysis of 1,300 stool specimens collected from pediatric patients, PVs were detected at 0...
September 24, 2016: Journal of Medical Virology
https://www.readbyqxmd.com/read/27650154/bocavirus-respiratory-infections
#15
David Isaacs
No abstract text is available yet for this article.
September 2016: Journal of Paediatrics and Child Health
https://www.readbyqxmd.com/read/27519364/a-novel-bocavirus-from-domestic-mink-china
#16
Shixing Yang, Yan Wang, Wang Li, Zhaobin Fan, Lili Jiang, Yuan Lin, Xingli Fu, Quan Shen, Zixuan Sun, Xiaochun Wang, Xutao Deng, Wen Zhang, Eric Delwart
Bocaviruses have been found in the feces of humans and a variety of animals, including pigs, cattle, dogs, gorillas, cats, and sea lions. Here, we have characterized the almost complete genome (5224 nt) of a novel bocavirus from feces of domestic minks, which has been provisionally named mink bocavirus. The NS1 protein of mink bocavirus shared 36.9-52 % amino acid sequence identities with those of other known bocaviruses and phylogenetically clustered with bocaviruses from other carnivores. According to the genetic distance-based criteria, mink bocavirus qualifies as a novel species of bocavirus...
December 2016: Virus Genes
https://www.readbyqxmd.com/read/27490242/prevalence-and-phylogenetic-analysis-of-human-bocaviruses-1-4-in-pediatric-patients-with-various-infectious-diseases
#17
Min Zhao, Runan Zhu, Yuan Qian, Jie Deng, Fang Wang, Yu Sun, Huijin Dong, Liying Liu, Liping Jia, Linqing Zhao
OBJECTIVES: Viral infections caused by human bocaviruses 1-4 (HBoV1-4) are more complicated than previously believed. A retrospective, large-scale study was undertaken to explore the prevalence of HBoV1-4 in pediatric patients with various infectious diseases and delineate their phylogenetic characteristics. METHODS: Clinical samples from four specimen types, including 4,941 respiratory, 2,239 cerebrospinal fluid (CSF), 2,619 serum, and 1,121 fecal specimens, collected from pediatric patients with various infectious diseases were screened for HBoV1-4...
2016: PloS One
https://www.readbyqxmd.com/read/27448821/development-and-validation-of-a-multiplex-conventional-pcr-assay-for-simultaneous-detection-and-grouping-of-porcine-bocaviruses
#18
Xiaowen Zheng, Gaopeng Liu, Tanja Opriessnig, Zining Wang, Zongqi Yang, Yonghou Jiang
Porcine bocavirus (PBoV), a newly described porcine parvovirus, has received attention because it can be commonly identified in clinically affected pigs including pigs with post-weaning multisystemic wasting syndrome (PWMS) and pigs with diarrhea. In recent years, novel PBoVs have been identified and were classified into three genogroups, but the ability to detect and classify these novel PBoVs is not comprehensive to date. In this study, a multiplex conventional PCR assay for simultaneous detection and grouping of PBoVs was developed by screening combinations of mixed primer pairs followed by optimization of the PCR conditions...
October 2016: Journal of Virological Methods
https://www.readbyqxmd.com/read/27412553/-identification-of-bocavirus-infection-in-a-young-child-with-pneumonia-using-a-pan-microbial-microarray
#19
Shu-Mei Peng, Hua Deng, Min-Min Li, Shu-Jun Huang, Wei-Ping Zhou, Ling-Long Lu, Dong-Ping Huang, Wen-Cheng Li, Ying Lin, Qiu-Ping Chen, Liang Zhang
No abstract text is available yet for this article.
July 2016: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/27388369/genetic-characterization-of-feline-bocavirus-detected-in-cats-in-japan
#20
Tomomi Takano, Yoshihiro Takadate, Tomoyoshi Doki, Tsutomu Hohdatsu
Feline bocavirus (FBoV) has been classified into three genotypes (FBoV1-FBoV3). FBoVs are mainly detected in feces. In the present study, we collected rectal swabs from cats in Japan and examined the samples for the presence of FBoV. The FBoV infection rate was 9.9 % in 101 cats. No significant association was observed between FBoV infection and clinical symptoms. Based on the full-length NS1 protein, the three strains of FBoVs detected in the present study shared high homologies with the genotype 2 FBoV POR1 strain...
October 2016: Archives of Virology
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