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https://www.readbyqxmd.com/read/29122866/genome-sequence-of-a-porcine-bocavirus-detected-in-feces-of-domestic-minks-in-china
#1
Yan Wang, Jing Zhao, Min Zheng, Zhijian Liu, Jiaqi Yuan, Jieji Zhao, Quan Shen, Zhaobin Fan, Lili Jiang, Shixing Yang
We report here the genome sequence of porcine bocavirus strain PBov-JZ08, which was isolated from mink feces in China. Sequence analysis implied that PBov-JZ08 clustered with three porcine bocaviruses.
November 9, 2017: Genome Announcements
https://www.readbyqxmd.com/read/29028159/serologically-diagnosed-acute-human-bocavirus-1-infection-in-childhood-community-acquired-pneumonia
#2
Amanda C Nascimento-Carvalho, Ana-Luisa Vilas-Boas, Maria-Socorro H Fontoura, Man Xu, Tytti Vuorinen, Maria Söderlund-Venermo, Olli Ruuskanen, Cristiana M Nascimento-Carvalho
AIM: To assess the role of human bocavirus 1 (HBoV1) as a causative agent of non-severe community-acquired pneumonia (CAP) in children. METHODS: Patients aged 2-59 months with non-severe CAP (respiratory complaints and radiographic pulmonary infiltrate/consolidation) attending a University Hospital in Salvador, Brazil were enrolled in a prospective cohort. From 820 recruited children in a clinical trial (ClinicalTrials.gov NCT01200706), nasopharyngeal aspirate (NPA), and acute and convalescent serum samples were obtained from 759 (92...
October 13, 2017: Pediatric Pulmonology
https://www.readbyqxmd.com/read/29021400/human-parvovirus-infection-of-human-airway-epithelia-induces-pyroptotic-cell-death-via-inhibiting-apoptosis
#3
Xuefeng Deng, Wei Zou, Min Xiong, Zekun Wang, John F Engelhardt, Shui Qing Ye, Ziying Yan, Jianming Qiu
Human bocavirus 1 (HBoV1) is a human parvovirus that causes acute respiratory tract infections in young children. In this study, we confirmed that, when polarized/well-differentiated human airway epithelia are infected with HBoV1 in vitro, they develop damage characterized by barrier function disruption and cell hypotrophy. Cell death mechanism analyses indicated that the infection induced pyroptotic cell death characterized by caspase-1 activation. Unlike infections with other parvoviruses, HBoV1 infection did not activate the apoptotic or necroptotic cell death pathway...
October 11, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28934425/human-bocavirus-capsid-messenger-rna-detection-in-children-with-pneumonia
#4
MULTICENTER STUDY
Robert Schlaberg, Krow Ampofo, Keith D Tardif, Chris Stockmann, Keith E Simmon, Weston Hymas, Steven Flygare, Brett Kennedy, Anne Blaschke, Karen Eilbeck, Mark Yandell, Jon A McCullers, Derek J Williams, Kathryn Edwards, Sandra R Arnold, Anna Bramley, Seema Jain, Andrew T Pavia
Background: The role of human bocavirus (HBoV) in respiratory illness is uncertain. HBoV genomic DNA is frequently detected in both ill and healthy children. We hypothesized that spliced viral capsid messenger RNA (mRNA) produced during active replication might be a better marker for acute infection. Methods: As part of the Etiology of Pneumonia in the Community (EPIC) study, children aged <18 years who were hospitalized with community-acquired pneumonia (CAP) and children asymptomatic at the time of elective outpatient surgery (controls) were enrolled...
September 15, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28924489/identification-of-an-enterovirus-recombinant-with-a-torovirus-like-gene-insertion-during-a-diarrhea-outbreak-in-fattening-pigs
#5
Nádia Conceição-Neto, Sebastiaan Theuns, Tingting Cui, Mark Zeller, Claude Kwe Yinda, Isaura Christiaens, Elisabeth Heylen, Marc Van Ranst, Sebastien Carpentier, Hans J Nauwynck, Jelle Matthijnssens
Diarrhea outbreaks in pig farms have raised major concerns in Europe and USA, as they can lead to dramatic pig losses. During a suspected outbreak in Belgium of porcine epidemic diarrhea virus (PEDV), we performed viral metagenomics to assess other potential viral pathogens. Although PEDV was detected, its low abundance indicated that other viruses were involved in the outbreak. Interestingly, a porcine bocavirus and several enteroviruses were most abundant in the sample. We also observed the presence of a porcine enterovirus genome with a gene insertion, resembling a C28 peptidase gene found in toroviruses, which was confirmed using re-sequencing, bioinformatics, and proteomics approaches...
July 2017: Virus Evolution
https://www.readbyqxmd.com/read/28922613/the-etiology-of-community-acquired-pneumonia-among-children-under-5%C3%A2-years-of-age-in-mainland-china-2001-2015-a-systematic-review
#6
Guijun Ning, Xuxia Wang, Dan Wu, Zundong Yin, Yixing Li, Huaqing Wang, Weizhong Yang
BACKGROUND: The aim of this systematic review was to examine the etiology of community-acquired pneumonia (CAP) among Chinese children younger than 5 y and provide evidence for further cost-effectiveness analyses for vaccine development, diagnostic strategies and empirical treatments. METHODS: The literature review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement. Data were obtained by searching PubMed, Embase, Web-of Science, and the Chinese databases Wanfang Data and China National Knowledge Infrastructure...
September 18, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28910409/risk-of-acute-gastroenteritis-associated-with-human-bocavirus-infection-in-children-a-systematic-review-and-meta-analysis
#7
REVIEW
Ri De, Liying Liu, Yuan Qian, Runan Zhu, Jie Deng, Fang Wang, Yu Sun, Huijin Dong, Liping Jia, Linqing Zhao
Human bocaviruses (HBoVs), which were first identified in 2005 and are composed of genotypes 1-4, have been increasingly detected worldwide in pediatric patients with acute gastroenteritis. To investigate if HBoV infection is a risk factor of acute gastroenteritis in children younger than 5 years old, we searched PubMed, Embase (via Ovid), the Chinese Biomedical Literature Database (CBM), and the Cochrane Library for studies assessing the prevalence of HBoVs in individuals from Oct 25, 2005 to Oct 31, 2016...
2017: PloS One
https://www.readbyqxmd.com/read/28883450/virome-analysis-for-identification-of-novel-mammalian-viruses-in-bats-from-southeast-china
#8
Dan Hu, Changqiang Zhu, Yi Wang, Lele Ai, Lu Yang, Fuqiang Ye, Chenxi Ding, Jiafeng Chen, Biao He, Jin Zhu, Hui Qian, Wenrong Xu, Youjun Feng, Weilong Tan, Changjun Wang
Bats have been shown as important mammal resevoirs to carry a variety of zoonotic pathogens. To analyze pathogenic species in bats from southeast coastal regions of China, we performed metagenomic sequencing technology for high throughput sequencing of six sentinels from southeast coastal area of China. We obtained 5,990,261 high quality reads from intestine and lung tissue of 235 bats, including 2,975,371 assembled sequences. 631,490 reads predicted overlapping sequences for the open reading frame (ORF), which accounts for 2...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28876460/viral-etiology-of-sporadic-cases-of-parotitis-among-children-in-korea-during-2013-2014
#9
Hae J Kang, Sun H Kim, Jae K Chung, Soon W Lee, Seung B Choi, Hye E Eom, Ok Park, Kisoon Kim, Sung S Kim
Recent years have seen a high incidence of mumps, which is generally diagnosed based on clinical features, especially parotitis, without laboratory confirmation in Korea. To better understand the epidemiology of mumps in Korean children, we investigated sporadic suspected mumps cases with parotitis. In total, 237 buccal swabs or throat swabs collected from children with parotitis who had been clinically diagnosed with mumps were tested using real-time PCR for the detection of six viruses (Epstein-Barr virus, Human herpesvirus 6, Mumps virus, Human parainfluenza virus-1, -2, -3, Human adenovirus, Human bocavirus)...
September 6, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28854225/enteric-viruses-in-hiv-1-seropositive-and-hiv-1-seronegative-children-with-diarrheal-diseases-in-brazil
#10
Silvana Augusta Rodrigues Portes, Filipe Anibal Carvalho-Costa, Monica Simões Rocha, Tulio Machado Fumian, Adriana Gonçalves Maranhão, Rosane Maria de Assis, Maria da Penha Trindade Pinheiro Xavier, Myrna Santos Rocha, Marize Pereira Miagostovich, José Paulo Gagliardi Leite, Eduardo de Mello Volotão
Diarrheal diseases (DD) have distinct etiological profiles in immune-deficient and immune-competent patients. This study compares detection rates, genotype distribution and viral loads of different enteric viral agents in HIV-1 seropositive (n = 200) and HIV-1 seronegative (n = 125) children hospitalized with DD in Rio de Janeiro, Brazil. Except for group A rotavirus (RVA), which were detected through enzyme immunoassay, the other enteric viruses (norovirus [NoV], astrovirus [HAstV], adenovirus [HAdV] and bocavirus [HBoV]) were detected through PCR or RT-PCR...
2017: PloS One
https://www.readbyqxmd.com/read/28796554/adeno-associated-virus-human-bocavirus-1-chimeric-vectors-ferreting-out-their-role-in-airway-gene-therapy
#11
Terence R Flotte
No abstract text is available yet for this article.
August 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/28777951/human-bocavirus-1-infection-of-caco-2-cell-line-cultures
#12
Lucía María Ghietto, Ana Paola Toigo D'Angelo, Franco Agustin Viale, María Pilar Adamo
Human bocavirus 1 (HBoV1) is a parvovirus associated with pneumonia in infants. It has been detected in different tissues, including colorectal tumors. In this study, we investigated whether Caco-2 cell line, derived from human colon cancer, can be utilized as a model for HBoV1 replication. We demonstrate HBoV1 replication in Caco-2 cultures supplemented with DEAE-dextran after inoculation with respiratory material from infected patients presenting with acute respiratory infection. A viral cycle of rapid development is displayed...
October 2017: Virology
https://www.readbyqxmd.com/read/28763071/prevalence-of-respiratory-viruses-in-wheezing-children-not-older-than-24-months-of-age
#13
Martín Bedolla-Barajas, Hilda Montero, Jaime Morales-Romero, Adriana Landa-Cardeña, Jahaziel Díaz, Netzahualpilli Delgado-Figueroa, Luis Gustavo Orozco-Alatorre
INTRODUCTION: Wheezing in children not older than 24 months of age is a frequent event, and viruses are usually the causative agents. The aim of the study was to estimate the prevalence of respiratory viruses in wheezing children who were not older than 24 months of age and who had no history of asthma. METHODS: Fifty-five Mexican children were included in an analytical cross-sectional study. Nasal secretions were obtained by using sterile rayon-tipped applicators to identify the virus by polymerase chain reaction or reverse transcription-polymerase chain reaction: adenovirus, bocavirus, human rhinovirus, influenza virus type A, human metapneumovirus, parainfluenza, rhinovirus, and respiratory syncytial virus...
May 2017: Gaceta Médica de México
https://www.readbyqxmd.com/read/28731923/human-bocavirus-can-it-trigger-hemophagocytic-lymphohistiocytosis
#14
Sevgen Tanir Basaranoglu, Kubra Aykac, Yasemin Ozsurekci, Inci Bajin, Betul Tavil, Fatma Gumruk, Mehmet Ceyhan
With improvements in molecular diagnostic methods, report of Human bocavirus (HBoV) as an etiologic agent in many studies on viral respiratory and gastrointestinal infections has been increasing. Two pediatric patients who presented with secondary hemophagocytic lymphohistiocytosis were examined for etiologic causes, including viruses. Whole bacterial and fungal cultures and viral serological studies were negative. Viral polymerase chain reaction of nasopharyngeal secretions showed HBoV. One was successfully treated with intravenous immunoglobulins, whereas the other died with multiorgan failure...
November 2017: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/28719504/severe-lower-respiratory-tract-infection-caused-by-human-bocavirus-1-in-an-infant
#15
Vesa Eskola, Man Xu, Maria Söderlund-Venermo
We report a case of human bocavirus 1 (HBoV1) bronchiolitis that led to life-threatening respiratory failure in a 9-month-old boy with no other pathogens detected. The virus-specific diagnosis was confirmed with the detection of HBoV1 DNA in respiratory samples and both DNA and IgM and IgG to HBoV1 in serum samples.
November 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28716110/phylogenic-analysis-of-human-bocavirus-detected-in-children-with-acute-respiratory-infection-in-yaounde-cameroon
#16
Sebastien Kenmoe, Marie-Astrid Vernet, Mohamadou Njankouo-Ripa, Véronique Beng Penlap, Astrid Vabret, Richard Njouom
OBJECTIVE: Human Bocavirus (HBoV) was first identified in 2005 and has been shown to be a common cause of respiratory infections and gastroenteritis in children. In a recent study, we found that 10.7% of children with acute respiratory infections (ARI) were infected by HBoV. Genetic characterization of this virus remains unknown in Central Africa, particularly in Cameroon Leeding us to evaluate the molecular characteristics of HBoV strains in Cameroonian children with ARI. RESULTS: Phylogenetic analysis of partial HBoV VP1/2 sequences showed a low level of nucleotide variation and the circulation of HBoV genotype 1 (HBoV-1) only...
July 17, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28708249/the-impact-of-anthropogenic-pressure-on-the-virological-quality-of-water-from-the-tiber-river-italy
#17
G La Rosa, I Sanseverino, S Della Libera, M Iaconelli, V E V Ferrero, A Barra Caracciolo, T Lettieri
The objective of the present study was to assess the occurrence of major waterborne enteric viruses (enterovirus, norovirus, adenovirus, rotavirus, hepatitis A and E virus) along the Tiber River in Italy, in areas affected by different kinds of anthropogenic pressure (agricultural, urban, industrial and pristine). Moreover, in light of the recent abundant detection of human bocavirus in urban wastewater samples in Italy, the occurrence of this virus was also assessed. Virus detection was based on nested PCR followed by sequencing, and on real-time PCR...
October 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28673837/respiratory-viruses-detected-in-mexican-children-younger-than-5-years-old-with-community-acquired-pneumonia-a-national-multicenter-study
#18
Rosa María Wong-Chew, Miguel L García-León, Daniel E Noyola, Luis F Perez Gonzalez, Jesús Gaitan Meza, Alberto Vilaseñor-Sierra, Gerardo Martinez-Aguilar, Victor Hugo Rivera-Nuñez, Oscar A Newton-Sánchez, Verónica Firo-Reyes, Carlos N Del Río-Almendarez, Alejandra Pamela González-Rodríguez, Enrique R Ortiz-García, Susana Navarrete-Navarro, Carmen Soria-Rodríguez, Adoniram Carrasco-Castillo, Eneida Sánchez-Medina, Irma López-Martínez, Teresa Hernández-Andrade, Celia M Alpuche-Aranda, José I Santos-Preciado
BACKGROUND: Acute respiratory infections are the leading cause of mortality in children worldwide, especially in developing countries. Pneumonia accounts for 16% of all deaths of children under 5 years of age and was the cause of death of 935000 children in 2015. Despite its frequency and severity, information regarding its etiology is limited. The aim of this study was to identify respiratory viruses associated with community-acquired pneumonia (CAP) in children younger than 5 years old...
June 30, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28672995/human-bocavirus-induces-apoptosis-and-autophagy-in-human-bronchial-epithelial-cells
#19
You-Ping Deng, Ying-Juan Liu, Zhan-Qiu Yang, Yan-Jun Wang, Bing-Yan He, Pin Liu
Human bocavirus (HBoV) is classified in the Bocavirus genus within the Parvoviridae family, first identified from children with respiratory diseases. Previous studies have investigated the stimulating effect of HBoV on cell apoptosis and autophagy. In the present study, human bronchial epithelial cells (HBECs) were utilized to examine the mechanism of HBoV recombination expressing vector (pWHL-1) on the promotion of cell apoptosis and autophagy. The results from the present study indicated that pWHL-1 inhibited the proliferation of HBECs in a time-dependent manner...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28662150/a-viral-metagenomic-approach-on-a-non-metagenomic-experiment-mining-next-generation-sequencing-datasets-from-pig-dna-identified-several-porcine-parvoviruses-for-a-retrospective-evaluation-of-viral-infections
#20
Samuele Bovo, Gianluca Mazzoni, Anisa Ribani, Valerio Joe Utzeri, Francesca Bertolini, Giuseppina Schiavo, Luca Fontanesi
Shot-gun next generation sequencing (NGS) on whole DNA extracted from specimens collected from mammals often produces reads that are not mapped (i.e. unmapped reads) on the host reference genome and that are usually discarded as by-products of the experiments. In this study, we mined Ion Torrent reads obtained by sequencing DNA isolated from archived blood samples collected from 100 performance tested Italian Large White pigs. Two reduced representation libraries were prepared from two DNA pools constructed each from 50 equimolar DNA samples...
2017: PloS One
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