Tina Bagratuni, Foteini Aktypi, Ourania Theologi, Maria Sakkou, Kleio Maria Verrou, Nefeli Mavrianou-Koutsoukou, Dimitrios Patseas, Christine Liacos, Stamatia Skourti, Alexandra Papadimou, Kostantina Taouxi, Foteini Theodorakakou, Georgios Kollias, Petros Sfikakis, Evangelos Terpos, Meletios A Dimopoulos, Efstathios Kastritis
Waldenström macroglobulinemia (WM) is characterized by the expansion of clonal lymphoplasmacytic cells; the MYD88L265P somatic mutation is found in >90% of patients, but malignant B cells may still display intra-clonal heterogeneity. To assess clonal heterogeneity in WM, we generated and performed single-cell RNA sequencing of CD19+ sorted cells from five patients with MYD88 L265P and two patients with MYD88 WT genotype as well as two healthy donors. We identified distinct transcriptional patterns in the clonal subpopulations not only between the two genetically distinct WM subgroups but also among MYD88 L265P patients, which affected the B cell composition in the different subgroups...
February 2024: HemaSphere