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https://www.readbyqxmd.com/read/27913828/engineering-and-evolution-of-saccharomyces-cerevisiae-to-produce-biofuels-and-chemicals
#1
Timothy L Turner, Heejin Kim, In Iok Kong, Jing-Jing Liu, Guo-Chang Zhang, Yong-Su Jin
To mitigate global climate change caused partly by the use of fossil fuels, the production of fuels and chemicals from renewable biomass has been attempted. The conversion of various sugars from renewable biomass into biofuels by engineered baker's yeast (Saccharomyces cerevisiae) is one major direction which has grown dramatically in recent years. As well as shifting away from fossil fuels, the production of commodity chemicals by engineered S. cerevisiae has also increased significantly. The traditional approaches of biochemical and metabolic engineering to develop economic bioconversion processes in laboratory and industrial settings have been accelerated by rapid advancements in the areas of yeast genomics, synthetic biology, and systems biology...
December 3, 2016: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/27909055/molecular-identification-of-d-ribulokinase-in-budding-yeast-and-mammals
#2
Charandeep Singh, Enrico Glaab, Carole L Linster
Proteomes of even well characterized organisms still contain a high percentage of proteins with unknown or uncertain molecular and/or biological function. A significant fraction of those proteins are predicted to have catalytic properties. Here we aimed at identifying the function of the Saccharomyces cerevisiae Ydr109c protein and of its human homolog FGGY, both of which belong to the broadly conserved FGGY family of carbohydrate kinases. Functionally identified members of this family phosphorylate 3- to 7-carbon sugars or sugar derivatives, but the endogenous substrate of S...
December 1, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27903830/early-life-sugar-consumption-affects-the-rat-microbiome-independently-of-obesity
#3
Emily E Noble, Ted M Hsu, Roshonda B Jones, Anthony A Fodor, Michael I Goran, Scott E Kanoski
BACKGROUND: The gut microbiome has been implicated in various metabolic and neurocognitive disorders and is heavily influenced by dietary factors, but there is a paucity of research on the effects of added sugars on the gut microbiome. OBJECTIVE: With the use of a rodent model, our goal was to determine how added-sugar consumption during the juvenile and adolescent phase of development affects the gut microbiome. METHODS: Forty-two juvenile male Sprague-Dawley rats [postnatal day (PND) 26; 50-70 g] were given access to 1 of 3 different 11%-carbohydrate solutions designed to model a range of monosaccharide ratios commonly consumed in sugar-sweetened beverages: 1) 35% fructose:65% glucose, 2) 50% fructose:50% glucose, 3) 65% fructose:35% glucose, and 4) control (no sugar)...
November 30, 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/27903226/protective-effects-of-caffeic-acid-and-the-alzheimer-s-brain-an-update
#4
Solomon Habtemariam
Caffeic acid (CA) and related phenylpropanoic acids are ubiquitous natural products of the shikimic acid pathway origin. Due to the presence of diorthohydroxyl aromatic (catecholic) moiety, CA is not only one of the most potent antioxidant phenyl propanoids but also display numerous other pharmacological effects ranging from antiinflammatory to anticancer effects. Recent studies also demonstrated that CA both in its free form or conjugated with other groups such as quinic acid and sugars display profound effects in the brain including protection from toxicity induced by a variety of agents and/or experimental models of Alzheimer's disease (AD)...
November 29, 2016: Mini Reviews in Medicinal Chemistry
https://www.readbyqxmd.com/read/27901039/early-factors-related-to-carbohydrate-and-fat-intake-at-8-and-12-months-results-from-the-eden-mother-child-cohort
#5
W L Yuan, S Nicklaus, S Lioret, C Lange, A Forhan, B Heude, M-A Charles, B de Lauzon-Guillain
BACKGROUND/OBJECTIVES: Few studies have examined the factors explaining the variability in fat and carbohydrate intake during infancy. We aimed to describe infants' fat and carbohydrate intake and analyse the associations with infant and maternal characteristics and feeding practices. SUBJECTS/METHODS: This study included 1275 infants aged 8 months from the French EDEN mother-child cohort. Carbohydrate intake, fat intake, added fat (vegetable oils and animal fats) and added sugar (honey, white sugar, brown sugar, jam and sweetened beverages) consumption were calculated at 8 and 12 months...
November 30, 2016: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/27900664/improvement-production-of-hyaluronic-acid-by-streptococcus-zooepidemicus-in-sugarcane-molasses
#6
Nicole Caldas Pan, Hanny Cristina Braga Pereira, Maria de Lourdes Corradi da Silva, Ana Flora Dalberto Vasconcelos, Maria Antonia Pedrine Colabone Celligoi
Microbial hyaluronic acid (HA) production has been preferred rather than extraction from animal tissue for medical and cosmetic applications. In this context, to obtain an economically competitive HA production by Streptococcus zooepidemicus, culture conditions were studied to improve the polymer production in sugarcane molasses. The highest HA production by S. zooepidemicus ATCC 39920 achieved was 2.825 g. L(-1) in a 4.5 L bioreactor with controlled pH (8.0) and medium containing molasses (85.35 g.L(-1) total sugar) pretreated with activated charcoal and yeast extract (50 g...
November 30, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27895707/fungal-fermentation-on-anaerobic-digestate-for-lipid-based-biofuel-production
#7
Yuan Zhong, Zhiguo Liu, Christine Isaguirre, Yan Liu, Wei Liao
BACKGROUND: Anaerobic digestate is the effluent from anaerobic digestion of organic wastes. It contains a significant amount of nutrients and lignocellulosic materials, even though anaerobic digestion consumed a large portion of organic matters in the wastes. Utilizing the nutrients and lignocellulosic materials in the digestate is critical to significantly improve efficiency of anaerobic digestion technology and generate value-added chemical and fuel products from the organic wastes...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27893829/sansevieria-roxburghiana-schult-schult-f-family-asparagaceae-attenuates-type-2-diabetes-and-its-associated-cardiomyopathy
#8
Niloy Bhattacharjee, Ritu Khanra, Tarun K Dua, Susmita Das, Bratati De, M Zia-Ul-Haq, Vincenzo De Feo, Saikat Dewanjee
BACKGROUND: Sansevieria roxburghiana Schult. & Schult. F. (Family: Asparagaceae) rhizome has been claimed to possess antidiabetic activity in the ethno-medicinal literature in India. Therefore, present experiments were carried out to explore the protective role of edible (aqueous) extract of S. roxburghiana rhizome (SR) against experimentally induced type 2 diabetes mellitus (T2DM) and its associated cardiomyopathy in Wistar rats. METHODS: SR was chemically characterized by GC-MS analysis...
2016: PloS One
https://www.readbyqxmd.com/read/27892582/lubrication-of-chocolate-during-oral-processing
#9
S A Rodrigues, N Selway, M P Morgenstern, L Motoi, J R Stokes, B J James
The structure of chocolate is drastically transformed during oral processing from a composite solid to an oil/water fluid emulsion. Using two commercial dark chocolates varying in cocoa solids content, this study develops a method to identify the factors that govern lubrication in molten chocolate and saliva's contribution to lubrication following oral processing. In addition to chocolate and its individual components, simulated boluses (molten chocolate and phosphate buffered saline), in vitro boluses (molten chocolate and whole human saliva) and ex vivo boluses (chocolate expectorated after chewing till the point of swallow) were tested...
November 28, 2016: Food & Function
https://www.readbyqxmd.com/read/27888729/fractionation-of-rapeseed-straw-by-hydrothermal-dilute-acid-pretreatment-combined-with-alkali-post-treatment-for-improving-its-enzymatic-hydrolysis
#10
Bo-Yang Chen, Bao-Cheng Zhao, Ming-Fei Li, Qiu-Yun Liu, Run-Cang Sun
The aim of the research was to evaluate the effect of combined treatments on fermentable sugar production from rapeseed straw. An optimum condition was found to be the combination of hydrothermal pretreatment at 180°C for 45min and post-treatment by 2% NaOH at 100°C for 2h, which was based on the quantity of monosaccharides released during enzymatic hydrolysis. As compared with the raw material without treatment, the combination of hydrothermal pretreatment and alkali post-treatment resulted in a significant increase of the saccharification rate by 5...
November 16, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27872661/production-of-l-lactic-acid-from-acid-pretreated-sugarcane-bagasse-using-bacillus-coagulans-dsm2314-in-a-simultaneous-saccharification-and-fermentation-strategy
#11
Edwin C van der Pol, Gerrit Eggink, Ruud A Weusthuis
BACKGROUND: Sugars derived from lignocellulose-rich sugarcane bagasse can be used as feedstock for production of l(+)-lactic acid, a precursor for renewable bioplastics. In our research, acid-pretreated bagasse was hydrolysed with the enzyme cocktail GC220 and fermented by the moderate thermophilic bacterium Bacillus coagulans DSM2314. Saccharification and fermentation were performed simultaneously (SSF), adding acid-pretreated bagasse either in one batch or in two stages. SSF was performed at low enzyme dosages of 10...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27871249/benign-clear-cell-sugar-tumor-of-the-lung-in-a-patient-with-birt-hogg-dub%C3%A3-syndrome-a-case-report
#12
Yoko Gunji-Niitsu, Toshio Kumasaka, Shigehiro Kitamura, Yoshito Hoshika, Takuo Hayashi, Hitoshi Tokuda, Riichiro Morita, Etsuko Kobayashi, Keiko Mitani, Mika Kikkawa, Kazuhisa Takahashi, Kuniaki Seyama
BACKGROUND: Birt-Hogg-Dubé (BHD) syndrome is a rare inherited autosomal genodermatosis and caused by germline mutation of the folliculin (FLCN) gene, a tumor suppressor gene of which protein product is involved in mechanistic target of rapamycin (mTOR) signaling pathway regulating cell growth and metabolism. Clinical manifestations in BHD syndrome is characterized by fibrofolliculomas of the skin, pulmonary cysts with or without spontaneous pneumothorax, and renal neoplasms. There has been no pulmonary neoplasm reported in BHD syndrome, although the condition is due to deleterious sequence variants in a tumor suppressor gene...
November 21, 2016: BMC Medical Genetics
https://www.readbyqxmd.com/read/27869696/pilot-scale-production-and-thermostability-improvement-of-the-m23-protease-pseudoalterin-from-the-deep-sea-bacterium-pseudoalteromonas-sp-cf6-2
#13
Jie Yang, Yang Yu, Bai-Lu Tang, Shuai Zhong, Mei Shi, Bin-Bin Xie, Xi-Ying Zhang, Bai-Cheng Zhou, Yu-Zhong Zhang, Xiu-Lan Chen
Pseudoalterin is the most abundant protease secreted by the marine sedimental bacterium Pseudoalteromonas sp. CF6-2 and is a novel cold-adapted metalloprotease of the M23 family. Proteases of the M23 family have high activity towards peptidoglycan and elastin, suggesting their promising biomedical and biotechnological potentials. To lower the fermentive cost and improve the pseudoalterin production of CF6-2, we optimized the fermentation medium by using single factor experiments, added 0.5% sucrose as a carbon source, and lowered the usage of artery powder from 1...
November 17, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27865913/tio2-uv-based-photocatalytic-pretreatment-of-wheat-straw-for-biogas-production
#14
Merlin Alvarado-Morales, Panagiotis Tsapekos, Muhammad Awais, Muhammad Gulfraz, Irini Angelidaki
The present study deals with the application of an advanced oxidation process combining UV irradiation in the presence of the photocatalyst titanium dioxide (TiO2), as an effective pretreatment method of wheat straw as means for increasing its biodegradability for increased biogas production by anaerobic digestion (AD). Especially attention was paid in oxidation of the lignin in straw, besides release the sugars from the lignocellulosic structure of straw. Specifically, four different TiO2 concentrations (0...
November 16, 2016: Anaerobe
https://www.readbyqxmd.com/read/27863001/dietary-quality-and-household-food-insecurity-among-mexican-children-and-adolescents
#15
Luis A Rodríguez, Verónica Mundo-Rosas, Ignacio Méndez-Gómez-Humarán, Rafael Pérez-Escamilla, Teresa Shamah-Levy
Seventy percent of Mexican households experience some level of food insecurity (FI). Studies have shown positive associations between FI and poor dietary quality. As far as it is known, this is the first time the Healthy Eating Index (HEI-2010) has been used to assess dietary quality of children and adolescents in Mexico, and to examine if FI is related to it. The objective of this research is to assess dietary quality and its association with FI among Mexican children and adolescents from a nationally representative cross-sectional sample...
November 14, 2016: Maternal & Child Nutrition
https://www.readbyqxmd.com/read/27843720/proteogenomic-analyses-indicate-bacterial-methylotrophy-and-archaeal-heterotrophy-are-prevalent-below-the-grass-root-zone
#16
Cristina N Butterfield, Zhou Li, Peter F Andeer, Susan Spaulding, Brian C Thomas, Andrea Singh, Robert L Hettich, Kenwyn B Suttle, Alexander J Probst, Susannah G Tringe, Trent Northen, Chongle Pan, Jillian F Banfield
Annually, half of all plant-derived carbon is added to soil where it is microbially respired to CO2. However, understanding of the microbiology of this process is limited because most culture-independent methods cannot link metabolic processes to the organisms present, and this link to causative agents is necessary to predict the results of perturbations on the system. We collected soil samples at two sub-root depths (10-20 cm and 30-40 cm) before and after a rainfall-driven nutrient perturbation event in a Northern California grassland that experiences a Mediterranean climate...
2016: PeerJ
https://www.readbyqxmd.com/read/27843253/acidogenic-potential-of-plain-milk-milk-with-sugar-milk-with-cornflakes-and-milk-cornflakes-with-sugar-a-comparative-study
#17
REVIEW
Sham S Bhat, Sundeep K Hegde, Vidya S Bhat, K M Ramya, Praveen S Jodalli
AIM: To compare the acidogenic potential of plain milk, milk with sugar, milk with cornflakes, and milk cornflakes with sugar by assessing the salivary pH. MATERIALS AND METHODS: The study was carried out on 40 school children of 8 to 12 years; 20 boys and 20 girls were randomly selected. The salivary pH was assessed before and after the consumption of milk; milk and sugar; milk and cornflakes; and milk, sugar, and cornflakes. Baseline unstimulated saliva was collected in sterile plastic tube and the pH was recorded...
July 2016: International Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/27829485/the-impact-of-doubling-dairy-or-plant-based-foods-on-consumption-of-nutrients-of-concern-and-proper-bone-health-for-adolescent-females
#18
Elieke Demmer, Christopher J Cifelli, Jenny A Houchins, Victor L Fulgoni
OBJECTIVE: To determine the effects of increasing plant-based foods v. dairy foods on energy and nutrients of concern in adolescent females via diet modelling exercises. DESIGN: Data from the National Health and Nutrition Examination Survey (NHANES) were used to compare nutrient intakes from usual diet with those from three dietary scenarios that increased current intakes by 100 % of the following: (i) plant-based foods; (ii) protein-rich plant-based foods; and (iii) milk, cheese and yoghurt...
November 10, 2016: Public Health Nutrition
https://www.readbyqxmd.com/read/27827899/relationship-between-added-sugars-consumption-and-chronic-disease-risk-factors-current-understanding
#19
REVIEW
James M Rippe, Theodore J Angelopoulos
Added sugars are a controversial and hotly debated topic. Consumption of added sugars has been implicated in increased risk of a variety of chronic diseases including obesity, cardiovascular disease, diabetes and non-alcoholic fatty liver disease (NAFLD) as well as cognitive decline and even some cancers. Support for these putative associations has been challenged, however, on a variety of fronts. The purpose of the current review is to summarize high impact evidence including systematic reviews, meta-analyses, and randomized controlled trials (RCTs), in an attempt to provide an overview of current evidence related to added sugars and health considerations...
November 4, 2016: Nutrients
https://www.readbyqxmd.com/read/27827482/glycation-induces-conformational-changes-in-the-amyloid-%C3%AE-peptide-and-enhances-its-aggregation-propensity-molecular-insights
#20
Asis K Jana, Kedar B Batkulwar, Mahesh J Kulkarni, Neelanjana Sengupta
The cytotoxicity of the amyloid beta (Aβ) peptide, implicated in the pathogenesis of Alzheimer's disease (AD), can be enhanced by its post-translational glycation, a series of non-enzymatic reactions with reducing sugars and reactive dicarbonyls. However, little is known about the underlying mechanisms that potentially enhance the cytotoxicity of the advanced glycation modified Aβ. In this work, fully atomistic molecular dynamics (MD) simulations are exploited to obtain direct molecular insights into the process of early Aβ self-assembly in the presence and absence of glycated lysine residues...
November 9, 2016: Physical Chemistry Chemical Physics: PCCP
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