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https://www.readbyqxmd.com/read/28945009/biomechanical-regulation-of-mesenchymal-stem-cells-for-cardiovascular-tissue-engineering
#1
REVIEW
Kayla Henderson, Andrew D Sligar, Victoria P Le, Jason Lee, Aaron B Baker
Mesenchymal stem cells (MSCs) are an appealing potential therapy for vascular diseases; however, many challenges remain in their clinical translation. While the use of biochemical, pharmacological, and substrate-mediated treatments to condition MSCs has been subjected to intense investigation, there has been far less exploration of using these treatments in combination with applied mechanical force for conditioning MSCs toward vascular phenotypes. This review summarizes the current understanding of the use of applied mechanical forces to differentiate MSCs into vascular cells and enhance their therapeutic potential for cardiovascular disease...
September 25, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28945007/nonmineralized-and-mineralized-collagen-scaffolds-induce-differential-osteogenic-signaling-pathways-in-human-mesenchymal-stem-cells
#2
Qi Zhou, Xiaoyan Ren, David Bischoff, Daniel W Weisgerber, Dean T Yamaguchi, Timothy A Miller, Brendan A C Harley, Justine C Lee
The instructive capabilities of extracellular matrix components in progenitor cell differentiation have recently generated significant interest in the development of bioinspired materials for regenerative applications. Previously, a correlation was described between the osteogenic capabilities of nanoparticulate mineralized collagen glycosaminoglycan scaffolds (MC-GAG) and an autogenous activation of small mothers against decapentaplegic ( Smad1/5) in the canonical bone morphogenetic protein receptor (BMPR) pathway with a diminished extracellular signal regulated kinase 1/2 (ERK1/2) activation when compared to nonmineralized collagen glycosaminoglycan scaffolds (Col-GAG)...
September 25, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28944999/systemic-redistribution-of-the-intramyocardially-injected-mesenchymal-stem-cells-by-repeated-remote-ischaemic-post-conditioning
#3
Qin Jiang, Tao Yu, Keli Huang, Hao Zhang, Zhe Zheng, Shengshou Hu
We investigated the effect of repeated remote ischaemic post-conditioning (RIPoC) on the organ distribution of the intramyocardially injected MSCs in rat myocardial ischemia model. Myocardial ischemia of adult female Sprague-Dawley rats was induced by 30-min. obstruction of the left anterior descending coronary artery. Repeated RIPoC was induced after ischemia with three cycles of 5-min. occlusion and reperfusion of the limb with the frequency of half a day, 1 or 2 days, respectively. Compared with that by single RIPoC, repeated RIPoC transiently reduced oxidative stress, lipid peroxidation and inflammation in ischaemic myocardium; the gene expression of stromal cell-derived factor-1 alpha (SDF-1α) was consistently induced by repeated RIPoC procedures...
September 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28944996/negative-pressure-wound-therapy-promotes-muscle-derived-stem-cell-osteogenic-differentiation-through-mapk-pathway
#4
Hong Liu, Xun Zheng, Liang Chen, Chao Jian, Xiang Hu, Yong Zhao, Zonghuan Li, Aixi Yu
Negative pressure wound therapy (NPWT) has been revealed to be effective in the treatment of open fractures, although the underlying mechanism is not clear. This article aimed to investigate the effects of NPWT on muscle-derived stem cell (MDSC) osteoblastic differentiation and the related potential mechanism. The cell proliferation rate was substantially increased in NPWT-treated MDSCs in comparison with a static group for 3 days. There was no observable effect on the apoptosis of MDSC treated with NPWT compared with the control group for 3 days...
September 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28944964/wnt-%C3%AE-catenin-and-erk-pathway-activation-a-possible-mechanism-of-photobiomodulation-therapy-with-light-emitting-diodes-that-regulate-the-proliferation-of-human-outer-root-sheath-cells
#5
Jung E Kim, Young J Woo, Ki M Sohn, Kwan H Jeong, Hoon Kang
BACKGROUND: Outer root sheath cells (ORSCs) play important roles in maintaining hair follicle structure and provide support for the bulge area. The hair growth promoting effects of photobiomodulation therapy (PBMT) have been reported, but the mechanisms for this in human ORCs (hORSCs) have rarely been studied. OBJECTIVE: The aim of this study was to investigate the effect of various wavelengths of light-emitting diode (LED) irradiation on human ORSCs (hORSCs). METHODS: LED irradiation effects on hORSC proliferation and migration were examined with MTT assay, BrdU incorporation assay and migration assays...
September 25, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28944953/nk-cells-produce-high-levels-of-il-10-early-after-allogeneic-stem-cell-transplantation-and-suppress-development-of-acute-gvhd
#6
Yl Tracey Chan, Jianmin Zuo, Charlotte Inman, Wayne Croft, Jusnara Begum, Joanne Croudace, Francesca Kinsella, Luke Maggs, Sandeep Nagra, Jane Nunnick, Ben Abbotts, Charles Craddock, Ram Malladi, Paul Moss
Natural killer (NK) cells rapidly reconstitute following allogeneic stem cell transplantation (allo-SCT), at the time when alloreactive T cell immunity is being established. We investigated very early NK cell reconstitution in 82 patients following T cell-depleted allo-SCT. NK cell number rapidly increased, exceeding T cell reconstitution such that the NK:T cell ratio was over 40 by day 14. NK cells at day 14 (NK-14) were donor-derived, intensely proliferating and expressed chemokine receptors targeted to lymphoid and peripheral tissue...
September 25, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/28944949/the-research-progress-of-mesenchymal-stem-cells-in-the-treatment-of-traumatic-brain-injury
#7
Gang Li, Yue Yang, Hua-Jiang Dong, Ling Lin
Traumatic brain injury (TBI) is the leading cause of mortality and morbidity in children and adults throughout the world. It is urgent to ameliorate TBI damage and reduce the disability and case-fatality rate. Stem cell therapy is another medical revolution after drug and surgical medication. Mesenchymal stem cells (MSCs) are a class of cells with significant self-renewal and multi-lineage differentiation properties and be favorable for the treatment of various diseases and injuries, it could be envisioned that MSCs transplantation may be a promising treatment for TBI...
July 16, 2017: Turkish Neurosurgery
https://www.readbyqxmd.com/read/28944943/effect-of-systemic-application-of-bone-marrow-derived-mesenchymal-stem-cells-on-healing-of-peripheral-nerve-injury-in-an-experimental-sciatic-nerve-injury-model
#8
Zahir Kizilay, Serdar Aktas, Nesibe Kahraman Cetin, Didem Bakay Ilhan, Gulfem Ersoy, Haydar Ali Erken
AIM: To investigate the effects of systemic application of bone marrow-derived mesenchymal stem cells in a compression model of peripheral nerve injury. MATERIAL AND METHODS: 24 male Wistar albino rats were randomly divided into 3 equal groups (n=8):Control (C),injury (I),and stem cell and injury (SI).The sciatic nerve of rats in the I and SI groups was subjected to clip compression for 5 minutes.Moreover,approximately 5x105 bone marrow-derived mesenchymal stem cells were given via tail vein of the rats in the SI group immediately after clip compression...
July 12, 2017: Turkish Neurosurgery
https://www.readbyqxmd.com/read/28944907/placenta%C3%A2-derived-mesenchymal-stem-cells-improve-airway-hyperresponsiveness-and-inflammation-in-asthmatic-rats-by-modulating-the-th17-treg-balance
#9
Yingying Li, Hongbo Li, Yinyin Cao, Fuling Wu, Wenbin Ma, Yuesi Wang, Shuzhen Sun
Mesenchymal stem cells (MSCs) possess reparative and immunoregulatory properties, representing a hope for stem cell‑based treatments. However, the mechanisms by which transplanted MSCs affect T helper (Th)17/regulatory T cell (Treg) balance in asthma patients remain unclear. The aim of the present study was to assess the therapeutic effects of human placenta MSCs (hPMSCs) in asthma, and explore the underlying mechanisms; in addition, the impact of hPMSCs transplantation on Th17/Treg balance in lymph and serum samples from asthmatic animals was evaluated...
September 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944869/promoting-potential-of-adipose-derived-stem-cells-on-peripheral-nerve-regeneration
#10
Jiayan Guo, Shu Guo, Yuxin Wang, Yanqiu Yu
The ultimate goal of treating peripheral nerve defects is reconstructing continuity of the nerve stumps to regain nerve conduction and functional recovery. Clinically, autologous nerve grafts and Schwann cell (SC) therapy have limitations, such as the need for secondary surgery, sacrifice of another nerve and donor site complication. Adipose derived stem cells (ADSCs) may promise to be ideal alternative cells of SCs. To explore the potential of ADSCs promoting peripheral nerve regeneration, the present study investigated the influences of ADSCs on proliferation and neurotrophic function of SCs using co‑culture model in vitro...
September 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944841/microrna%C3%A2-138%C3%A2-5p-regulates-neural-stem-cell-proliferation-and-differentiation-in%C3%A2-vitro-by-targeting-trip6-expression
#11
Juan Wang, Jixia Li, Jian Yang, Lianguo Zhang, Shane Gao, Fei Jiao, Maoli Yi, Jun Xu
Research on neural stem cells (NSCs) has recently focused on microRNAs (miRNAs), a class of small non‑coding RNAs that have crucial roles in regulating NSC proliferation and differentiation. In the present study, a quantitative‑polymerase chain reaction assay revealed that the expression of miRNA (miR)‑138‑5p was significantly decreased during neural differentiation of NSCs in vitro. Overexpression of miR‑138‑5p reduced NSC proliferation and increased NSC differentiation. Furthermore, suppression of miR‑138‑5p via transfection with a miRNA inhibitor enhanced NSC proliferation and attenuated NSC differentiation...
September 18, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944834/nf%C3%A2-%C3%AE%C2%BAb%C3%A2-mir15a%C3%A2-bfgf-vegfa-axis-contributes-to-the-impaired-angiogenic-capacity-of-bm%C3%A2-mscs-in-high-fat-diet%C3%A2-fed-mice
#12
Zhuoli Zhu, Xueqi Gan, Haiyang Yu
Potent paracrine properties, such as secretion of angiogenic cytokines and growth factors, have been considered essential for the function of mesenchymal stem cells (MSCs) in tissue regeneration and repair. The present study determined that bone marrow‑derived mesenchymal stem cells from mice fed a high fat diet (HFD) had reduced pro‑angiogenic capacity, as evident from the reduced expression of vascular endothelial growth factor A (VEGFA) and basic fibroblast growth factor (bFGF); therefore, a reduced number of branches was induced in the angiogenesis assay...
September 18, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944762/a-cell-laden-hybrid-fiber-hydrogel-composite-for-ligament-regeneration-with-improved-cell-delivery-and-infiltration
#13
Jeong Hwa Kim, Yeong-Jin Choi, Hee-Gyeong Yi, Joon Ho Wang, Dong-Woo Cho, Young Hun Jeong
Ligament, a fibrous connective tissue between bones, is a unique tissue in human anatomy because it has complex viscoelastic properties and is very tough. Moreover, it is an important tissue for regeneration because frequent injuries occur, but there are limited types of substitutes that can be used as a tissue replacement. In this study, we present a stem cell-laden fiber/hydrogel composite structure with a layered fibrous structure, which can enhance cell infiltration, topographical cue and mechanical properties...
September 25, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28944682/the-caffeoyl-phenylethanoid-glycosides-from-lindernia-ruellioides-and-their-anti-hbv-effects
#14
Jing-Chen Wei, Peng-Cheng Wang, Xian-Li Zhou, Kun Tang, Qin Luo, Qin Xu, Xue-Jiao Chen, Cheng-Qin Liang, Xu Chen
Five caffeoyl phenylethanoid glycosides, including two new ones linderruelliosides A (1) and B (2), were isolated from the leaves and stems of Lindernia ruellioides. Their structures were elucidated on the basis of spectroscopic methods, including extensive NMR and MS spectra. In addition, all these compounds were tested for their anti-HBV activity. Compounds 1, 3, and 4 showed anti-HBV activities, with IC50 values of 54.87, 30.74, and 69.02 μM for HBsAg and 26.70, 5.17, and 7.08 μM for HBeAg, respectively...
September 25, 2017: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/28944661/tempo-conjugated-gold-nanoparticles-for-reactive-oxygen-species-scavenging-and-regulation-of-stem-cell-differentiation
#15
Jingchao Li, Jing Zhang, Ying Chen, Naoki Kawazoe, Guoping Chen
Controlling the differentiation of human mesenchymal stem cells (hMSCs) shows a great potential in regenerative medicine. Because overproduced reactive oxygen species (ROS) have obvious inhibitory effect on the differentiation and functions of hMSCs, it is highly desirable to develop effective strategy for ROS scavenging and stem cell differentiation controlling. In this study, gold nanoparticles (Au NPs) with an average size of 40 nm were conjugated with 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) to endow them with ROS scavenging capacity while holding the beneficial effect of Au NPs...
September 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28944611/gene-therapy-with-the-trf1-telomere-gene-rescues-decreased-trf1-levels-with-aging-and-prolongs-mouse-health-span
#16
Aksinya Derevyanko, Kurt Whittemore, Ralph P Schneider, Verónica Jiménez, Fàtima Bosch, Maria A Blasco
The shelterin complex protects telomeres by preventing them from being degraded and recognized as double-strand DNA breaks. TRF1 is an essential component of shelterin, with important roles in telomere protection and telomere replication. We previously showed that TRF1 deficiency in the context of different mouse tissues leads to loss of tissue homeostasis owing to impaired stem cell function. Here, we show that TRF1 levels decrease during organismal aging both in mice and in humans. We further show that increasing TRF1 expression in both adult (1-year-old) and old (2-year-old) mice using gene therapy can delay age-associated pathologies...
September 24, 2017: Aging Cell
https://www.readbyqxmd.com/read/28944567/differentiation-of-mesenchymal-stem-cells-to-germ-like-cells-under-induction-of-sertoli-cell-conditioned-medium-and-retinoic-acid
#17
R Ghaem Maghami, T Mirzapour, A Bayrami
The aim of this research was to find a way to differentiate germ cells from umbilical cord Wharton's jelly mesenchymal stem cells (MSCs) to support in vitro spermatogenesis. A small piece of Wharton's jelly was cultured in high-glucose Dulbecco's modified Eagle's medium in present of 10% foetal calf serum. After the fourth passage, the cells were isolated and cultured in Sertoli cell-conditioned medium under induction of two different doses of retinoic acid (10(-5) , 10(-6)  m). The differentiation of MSC to germ-like cells was evaluated by expression of Oct4, Nanog, Plzf, Stra8 and Prm1 genes during different days of culture through qPCR...
September 25, 2017: Andrologia
https://www.readbyqxmd.com/read/28944484/the-stem-cell-club-a-model-for-unrelated-stem-cell-donor-recruitment
#18
Warren Fingrut, Simran Parmar, Ari Cuperfain, Kiran Rikhraj, Erin Charman, Emilie Ptak, Manjot Kahlon, Alice Graham, Susan Luong, Yongjun George Wang, Janice Yu, Neha Arora, Roopa Suppiah, Edward W Li, Anna Lee, Christopher Welsh, Menachem Benzaquen, Alicia Thatcher, Iman Baharmand, Aedan Ladd, Tanya Petraszko, David Allan, Hans Messner
BACKGROUND: Patients with blood, immune, or metabolic diseases may require a stem cell transplant as part of their treatment. However, 70% of patients do not have a suitable human leukocyte antigen match in their family, and need an unrelated donor. Individuals can register as potential donors at stem cell drives, where they provide consent and a tissue sample for human leukocyte antigen typing. The ideal donors are young, male, and from a diversity of ethnic backgrounds. However, in Canada, non-Caucasian males ages 17 to 35 years represent only 8...
September 24, 2017: Transfusion
https://www.readbyqxmd.com/read/28944322/advances-in-small-bowel-transplantation
#19
REVIEW
Alp Gürkan
Small bowel transplantation is a life-saving surgery for patients with intestinal failure. The biggest problem in intestinal transplantation is graft rejection. Graft rejection is the main reason for morbidity and mortality. Rejection has a negative effect on the survival of the graft. While 50%-75% of small bowel transplantation patients experience acute rejection, chronic rejection occurs in approximately 15% of patients. Immune monitoring is crucial after small bowel transplantation. Unlike other types of transplantation, there are no non-invasive or reliable markers to predict rejection in small bowel transplantation...
2017: Turk J Surg
https://www.readbyqxmd.com/read/28944287/detection-of-pentosidine-cross-links-in-cell-secreted-decellularized-matrices-using-time-resolved-fluorescence-spectroscopy
#20
Debika Mitra, Hussain Fatakdawala, Michael Nguyen-Truong, Amy Creecy, Jeffry Nyman, Laura Marcu, J Kent Leach
Hyperglycemia-mediated, nonenzymatic collagen cross-links such as pentosidine (PENT) can have deleterious effects on cellular interactions with the extracellular matrix (ECM). Present techniques to quantify PENT are limited, motivating the need for improved methods to study the accumulation and contribution of PENT toward diabetic clinical challenges such as impaired bone healing. Current methods for studying PENT are destructive, laborious, and frequently employ oversimplified collagen films that lack the complexity of the native ECM...
September 11, 2017: ACS Biomaterials Science & Engineering
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