keyword
https://read.qxmd.com/read/38475794/transcriptome-analysis-reveals-multiple-targets-of-erythritol-related-transcription-factor-euf1-in-unconventional-yeast-yarrowia-lipolytica
#21
JOURNAL ARTICLE
D A Rzechonek, M Szczepańczyk, I Borodina, C Neuvéglise, A M Mirończuk
BACKGROUND: Erythritol is a four-carbon polyol with an unclear role in metabolism of some unconventional yeasts. Its production has been linked to the osmotic stress response, but the mechanism of stress protection remains unclear. Additionally, erythritol can be used as a carbon source. In the yeast Yarrowia lipolytica, its assimilation is activated by the transcription factor Euf1. The study investigates whether this factor can link erythritol to other processes in the cell. RESULTS: The research was performed on two closely related strains of Y...
March 12, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38475513/development-of-an-nlr-id-toolkit-and-identification-of-novel-disease-resistance-genes-in-soybean
#22
JOURNAL ARTICLE
Wei Shao, Gongfu Shi, Han Chu, Wenjia Du, Zikai Zhou, Hada Wuriyanghan
The recognition of pathogen effectors through the nucleotide-binding leucine-rich repeat receptor (NLR) family is an important component of plant immunity. In addition to typical domains such as TIR, CC, NBS, and LRR, NLR proteins also contain some atypical integrated domains (IDs), the roles of which are rarely investigated. Here, we carefully screened the soybean ( Glycine max ) genome and identified the IDs that appeared in the soybean TNL-like proteins. Our results show that multiple IDs (36) are widely present in soybean TNL-like proteins...
February 28, 2024: Plants (Basel, Switzerland)
https://read.qxmd.com/read/38475508/genome-wide-identification-and-expression-analysis-reveal-bzip-transcription-factors-mediated-hormones-that-functions-during-early-somatic-embryogenesis-in-dimocarpus-longan
#23
JOURNAL ARTICLE
Tingkai Zhai, Shuoxian Lan, Luzhen Xv, Xueying Zhang, Xiangwei Ma, Zhuoyun Li, Jie Gao, Yukun Chen, Zhongxiong Lai, Yuling Lin
The basic leucine zip (bZIP) transcription factors (TFs) are a group of highly conserved gene families that play important roles in plant growth and resistance to adversity stress. However, studies on hormonal regulatory pathways and functional analysis during somatic embryogenesis (SE) in Dimocarpus longan is still unavailable. In this study, a total of 51 bZIP family members were systematically identified in the whole genome of longan, a comprehensive bioinformatics analysis of DlbZIP ( bZIP family members of D...
February 28, 2024: Plants (Basel, Switzerland)
https://read.qxmd.com/read/38474693/engineering-enhanced-antimicrobial-properties-in-%C3%AE-conotoxin-rgia-through-d-type-amino-acid-substitution-and-incorporation-of-lysine-and-leucine-residues
#24
JOURNAL ARTICLE
Minghe Wang, Zhouyuji Liao, Dongting Zhangsun, Yong Wu, Sulan Luo
Antimicrobial peptides (AMPs), acknowledged as host defense peptides, constitute a category of predominant cationic peptides prevalent in diverse life forms. This study explored the antibacterial activity of α-conotoxin RgIA, and to enhance its stability and efficacy, D-amino acid substitution was employed, resulting in the synthesis of nine RgIA mutant analogs. Results revealed that several modified RgIA mutants displayed inhibitory efficacy against various pathogenic bacteria and fungi, including Candida tropicalis and Escherichia coli ...
March 6, 2024: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/38474131/leucine-rich-repeat-in-polycystin-1-suppresses-cystogenesis-in-a-zebrafish-danio-rerio-model-of-autosomal-dominant-polycystic-kidney-disease
#25
JOURNAL ARTICLE
Biswajit Padhy, Mohammad Amir, Jian Xie, Chou-Long Huang
Mutations of PKD1 coding for polycystin-1 (PC1) account for most cases of autosomal-dominant polycystic kidney disease (ADPKD). The extracellular region of PC1 contains many evolutionarily conserved domains for ligand interactions. Among these are the leucine-rich repeats (LRRs) in the far N-terminus of PC1. Using zebrafish ( Danio rerio ) as an in vivo model system, we explored the role of LRRs in the function of PC1. Zebrafish expresses two human PKD1 paralogs, pkd1a and pkd1b . Knockdown of both genes in zebrafish by morpholino antisense oligonucleotides produced phenotypes of dorsal-axis curvature and pronephric cyst formation...
March 1, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38473980/the-upregulation-of-leucine-rich-repeat-containing-1-expression-activates-hepatic-stellate-cells-and-promotes-liver-fibrosis-by-stabilizing-phosphorylated-smad2-3
#26
JOURNAL ARTICLE
Yake Wang, Xiaolong Li, Xiaowen Guan, Zhe Song, Huanfei Liu, Zhenzhen Guan, Jianwei Wang, Lina Zhu, Di Zhang, Liang Zhao, Peitong Xie, Xiaoyi Wei, Ning Shang, Ying Liu, Zhongzhen Jin, Zhili Ji, Guifu Dai
Liver fibrosis poses a significant global health risk due to its association with hepatocellular carcinoma (HCC) and the lack of effective treatments. Thus, the need to discover additional novel therapeutic targets to attenuate liver diseases is urgent. Leucine-rich repeat containing 1 (LRRC1) reportedly promotes HCC development. Previously, we found that LRRC1 was significantly upregulated in rat fibrotic liver according to the transcriptome sequencing data. Herein, in the current work, we aimed to explore the role of LRRC1 in liver fibrosis and the underlying mechanisms involved...
February 27, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38473912/the-complete-chloroplast-genomes-of-bulbophyllum-orchidaceae-species-insight-into-genome-structure-divergence-and-phylogenetic-analysis
#27
JOURNAL ARTICLE
Yuwei Wu, Meng-Yao Zeng, Huan-Xin Wang, Siren Lan, Zhong-Jian Liu, Shibao Zhang, Ming-He Li, Yunxiao Guan
Bulbophyllum is one of the largest genera and presents some of the most intricate taxonomic problems in the family Orchidaceae, including species of ornamental and medical importance. The lack of knowledge regarding the characterization of Bulbophyllum chloroplast (cp) genomes has imposed current limitations on our study. Here, we report the complete cp genomes of seven Bulbophyllum species, including B . ambrosia , B . crassipes , B . farreri , B . hamatum , B . shanicum , B . triste , and B . violaceolabellum , and compared with related taxa to provide a better understanding of their genomic information on taxonomy and phylogeny...
February 25, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38473877/integrative-metabolomic-analysis-of-serum-and-selected-serum-exosomal-microrna-in-metastatic-castration-resistant-prostate-cancer
#28
JOURNAL ARTICLE
Daniel Evin, Andrea Evinová, Eva Baranovičová, Miroslava Šarlinová, Jana Jurečeková, Peter Kaplán, Hubert Poláček, Erika Halašová, Róbert Dušenka, Lukáš Briš, Martina Knoško Brožová, Monika Kmeťová Sivoňová
Metastatic castration-resistant prostate cancer (mCRPC) remains a lethal disease due to the absence of effective therapies. A more comprehensive understanding of molecular events, encompassing the dysregulation of microRNAs (miRs) and metabolic reprogramming, holds the potential to unveil precise mechanisms underlying mCRPC. This study aims to assess the expression of selected serum exosomal miRs (miR-15a, miR-16, miR-19a-3p, miR-21, and miR-141a-3p) alongside serum metabolomic profiling and their correlation in patients with mCRPC and benign prostate hyperplasia (BPH)...
February 23, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38473828/leucine-rich-glioma-inactivated-1-lgi1-protein-stimulates-proliferation-and-il-10-production-in-peripheral-blood-mononuclear-cells-of-patients-with-lgi1-antibody-mediated-autoimmune-encephalitis-in-vitro
#29
JOURNAL ARTICLE
Alexander Goihl, Dirk Reinhold, Annegret Reinhold, Burkhart Schraven, Peter Körtvelyessy
Limbic encephalitis (LE) due to anti-leucine-rich glioma-inactivated 1 (LGI1) antibodies is an autoimmune disease characterized by distinct clinical features unique to LGI1 LE, such as faciobrachial dystonic seizures. However, it is unclear whether an additional disease-related LGI1 antigen-specific T cell response is involved in the pathogenesis of this disease. To address this question, we studied the effect of recombinant LGI1 on the proliferation and effector-specific cytokine production (IFN-γ, IL-5, IL-10, and IL-17) of peripheral blood mononuclear cells (PBMCs) from patients with LGI1 LE and healthy controls...
February 23, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38473801/identification-of-bzip-transcription-factors-that-regulate-the-development-of-leaf-epidermal-cells-in-arabidopsis-thaliana-by-single-cell-rna-sequencing
#30
JOURNAL ARTICLE
Rui Wu, Zhixin Liu, Susu Sun, Aizhi Qin, Hao Liu, Yaping Zhou, Weiqiang Li, Yumeng Liu, Mengke Hu, Jincheng Yang, Jean-David Rochaix, Guoyong An, Luis Herrera-Estrella, Lam-Son Phan Tran, Xuwu Sun
Epidermal cells are the main avenue for signal and material exchange between plants and the environment. Leaf epidermal cells primarily include pavement cells, guard cells, and trichome cells. The development and distribution of different epidermal cells are tightly regulated by a complex transcriptional regulatory network mediated by phytohormones, including jasmonic acid, and transcription factors. How the fate of leaf epidermal cells is determined, however, is still largely unknown due to the diversity of cell types and the complexity of their regulation...
February 22, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38473764/inhibition-of-insulin-regulated-aminopeptidase-by-imidazo-1-5-%C3%AE-pyridines-synthesis-and-evaluation
#31
JOURNAL ARTICLE
Karin Engen, Thomas Lundbäck, Anubha Yadav, Sharathna Puthiyaparambath, Ulrika Rosenström, Johan Gising, Annika Jenmalm-Jensen, Mathias Hallberg, Mats Larhed
Inhibition of insulin-regulated aminopeptidase (IRAP) has been shown to improve cognitive functions in several animal models. Recently, we performed a screening campaign of approximately 10,000 compounds, identifying novel small-molecule-based compounds acting as inhibitors of the enzymatic activity of IRAP. Here we report on the chemical synthesis, structure-activity relationships (SAR) and initial characterization of physicochemical properties of a series of 48 imidazo [1,5-α]pyridine-based inhibitors, including delineation of their mode of action as non-competitive inhibitors with a small L-leucine-based IRAP substrate...
February 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38473173/supplementation-with-astragalus-root-powder-promotes-rumen-microbiota-density-and-metabolome-interactions-in-lambs
#32
JOURNAL ARTICLE
Pengyang Shao, Yuzhu Sha, Xiu Liu, Yanyu He, Fanxiong Wang, Jiang Hu, Jiqing Wang, Shaobin Li, Xiaowei Chen, Wenxin Yang, Qianling Chen, Min Gao
The gut microbiota is highly symbiotic with the host, and the microbiota and its metabolites are essential for regulating host health and physiological functions. Astragalus , as a feed additive, can improve animal immunity. However, the effects of Astragalus root powder on the rumen microbiota and their metabolites in lambs are not apparent. In this study, thirty healthy Hu sheep lambs with similar body weights (17.42 ± 2.02 kg) were randomly selected for the feeding experiment. Lambs were fed diets supplemented with 0...
March 2, 2024: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/38473150/estimation-of-protein-and-amino-acid-requirements-in-layer-chicks-depending-on-dynamic-model
#33
JOURNAL ARTICLE
Miao Liu, Zhi-Yuan Xia, Hong-Lin Li, Yu-Xuan Huang, Alainaa Refaie, Zhang-Chao Deng, Lv-Hui Sun
Four trials were conducted to establish a protein and amino acid requirement model for layer chicks over 0-6 weeks by using the analytical factorization method. In trial 1, a total of 90 one-day-old Jing Tint 6 chicks with similar body weight were selected to determine the growth curve, carcass and feather protein deposition, and amino acid patterns of carcass and feather proteins. In trials 2 and 3, 24 seven-day-old and 24 thirty-five-day-old Jing Tint 6 chicks were selected to determine the protein maintenance requirements, amino acid pattern, and net protein utilization rate...
February 29, 2024: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/38472837/competition-for-nitrogen-resources-an-explanation-of-the-effects-of-a-bioprotective-strain-metschnikowia-pulcherrima-on-the-growth-of-hanseniaspora-genus-in-oenology
#34
JOURNAL ARTICLE
Maëlys Puyo, Léa Scalabrino, Rémy Romanet, Scott Simonin, Géraldine Klein, Hervé Alexandre, Raphaëlle Tourdot-Maréchal
As a biological alternative to the antimicrobial action of SO2 , bioprotection has been proposed to winemakers as a means to limit or prevent grape musts microbial alteration. Competition for nitrogenous nutrients and for oxygen are often cited as potential explanations for the effectiveness of bioprotection. This study analyses the effect of a bioprotective M. pulcherrima strain on the growth of one H. valbyensis strain and one H. uvarum strain. Bioprotection efficiency was observed only against H. valbyensis inoculated at the two lowest concentrations...
February 27, 2024: Foods (Basel, Switzerland)
https://read.qxmd.com/read/38471937/-plant-diversity-changes-and-its-driving-factors-of-abandoned-land-at-different-restoration-stages-in-the-middle-of-the-qinling-mountains
#35
JOURNAL ARTICLE
Cheng-Long Yan, Yue Xue, Yi-Fei Wang, Hai-Bin Kang, De-Xiang Wang
The process of vegetation restoration is often accompanied by significant changes in aboveground plant diversity. To explore the driving mechanism of litter nutrient-soil nutrient-enzyme activity stoichiometry on aboveground vegetation change is of great importance for maintaining regional biodiversity conservation and ecological stability. Taking typical abandoned farmland of different restoration years (1, 8, 16, 31, and 50 a) in the Qinling Mountains as the research object, the variation characteristics of plant community diversity during vegetation restoration were analyzed through field investigation...
February 8, 2024: Huan Jing Ke Xue= Huanjing Kexue
https://read.qxmd.com/read/38471803/pharmacological-inhibition-of-lrrk2-exhibits-neuroprotective-activity-in-mouse-photothrombotic-stroke-model
#36
JOURNAL ARTICLE
Jeong-Ah Hwang, Seung Kyu Choi, Seong Hwan Kim, Dong Woon Kim
Leucine-rich repeat kinase 2 (LRRK2) mutations are the most common cause of Parkinson's disease (PD). Interestingly, recent studies have reported an increased risk of stroke in patients with PD harboring LRRK2 mutations, but there is no evidence showing the functional involvement of LRRK2 in stroke. Here, we found that LRRK2 kinase activity was significantly induced in the Rose-Bengal (RB) photothrombosis-induced stroke mouse model. Interestingly, stroke infarct volumes were significantly reduced, and neurological deficits were diminished by pharmacological inhibition of LRRK2 kinase activity using MLi-2, a brain-penetrant LRRK2 kinase inhibitor...
February 29, 2024: Experimental Neurobiology
https://read.qxmd.com/read/38471480/upstream-stimulating-factor-2-aggravates-neuropathic-pain-induced-in-spinal-nerve-ligation-induced-mice-via-regulating-snhg5-mir-181b-5p
#37
JOURNAL ARTICLE
Mi Chen, Yang Yang, Jiatian Cui, Li Qiu, Xiaohua Zou, Xianggang Zeng
BACKGROUND: Upstream stimulating factor 2 (USF2) belongs to basic-Helix-Loop-Helix-Leucine Zipper transcription factor family, regulating expression of genes involved in immune response or energy metabolism network. Role of USF2 in neuropathic pain was evaluated. METHODS: Mice were intraspinally injected with adenovirus for knockdown of USF2 (Ad-shUSF2), and then subjected to spinal nerve ligation (SNL) to induce neuropathic pain. Distribution and expression of USF2 was detected by western blot and immunofluorescence...
March 12, 2024: Developmental Neuroscience
https://read.qxmd.com/read/38471420/pepsin-mediated-inflammation-in-laryngopharyngeal-reflux-via-the-ros-nlrp3-il-1%C3%AE-signaling-pathway
#38
JOURNAL ARTICLE
Jia-Jie Tan, Yuan-Feng Dai, Fan Wang, Ze-Hong Lv, Li-Jun Huang, Ling-Yi Peng, Xiang-Ping Li
BACKGROUND: Laryngopharyngeal reflux (LPR) is one of the most common disorders in otorhinolaryngology, affecting up to 10% of outpatients visiting otolaryngology departments. In addition, 50% of hoarseness cases are related to LPR. Pepsin reflux-induced aseptic inflammation is a major trigger of LPR; however, the underlying mechanisms are unclear. The nucleotide-binding domain and leucine-rich repeat protein 3 (NLRP3) inflammasome has become an important bridge between stimulation and sterile inflammation and is activated by intracellular reactive oxygen species (ROS) in response to danger signals, leading to an inflammatory cascade...
March 11, 2024: Cytokine
https://read.qxmd.com/read/38470570/a-gradient-of-the-hd-zip-regulator-woolly-regulates-multicellular-trichome-morphogenesis-in-tomato
#39
JOURNAL ARTICLE
MinLiang Wu, XinXin Bian, ShouRong Hu, BenBen Huang, JingYuan Shen, YaDi Du, YanLi Wang, MengYuan Xu, HuiMin Xu, MeiNa Yang, Shuang Wu
Homeodomain (HD) proteins regulate embryogenesis in animals such as the fruit fly (Drosophila melanogaster), often in a concentration-dependent manner. HD-leucine zipper (Zip) IV family genes are unique to plants and often function in the L1 epidermal cell layer. However, our understanding of the roles of HD-Zip IV family genes in plant morphogenesis is limited. In this study, we investigated the morphogenesis of tomato (Solanum lycopersicum) multicellular trichomes, a type of micro-organ in plants. We found that a gradient of the HD-Zip IV regulator Woolly (Wo) coordinates spatially polarized cell division and cell expansion in multicellular trichomes...
March 12, 2024: Plant Cell
https://read.qxmd.com/read/38470397/pti-eti-synergistic-signal-mechanisms-in-plant-immunity
#40
REVIEW
Xiao-Qian Yu, Hao-Qiang Niu, Chao Liu, Hou-Ling Wang, Weilun Yin, Xinli Xia
Plants face a relentless onslaught from a diverse array of pathogens in their natural environment, to which they have evolved a myriad of strategies that unfold across various temporal scales. Cell surface pattern recognition receptors (PRRs) detect conserved elicitors from pathogens or endogenous molecules released during pathogen invasion, initiating the first line of defence in plants, known as pattern-triggered immunity (PTI), which imparts a baseline level of disease resistance. Inside host cells, pathogen effectors are sensed by the nucleotide-binding/leucine-rich repeat (NLR) receptors, which then activate the second line of defence: effector-triggered immunity (ETI), offering a more potent and enduring defence mechanism...
March 12, 2024: Plant Biotechnology Journal
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