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https://www.readbyqxmd.com/read/29457850/preparation-aroma-characteristics-and-volatile-compounds-of-flavorings-from-enzymatic-hydrolyzed-rice-bran-protein-concentrate
#1
Supeeraya Arsa, Chockchai Theerakulkait
BACKGROUND: Rice bran is by-product obtained from the rice milling industry. The aims of this research were to add value of rice bran by preparation of enzymatic hydrolyzed rice bran protein concentrate (HRPC) as a flavoring agent and the flavoring which was produced by HRPC has not been discovered. Different drying methods (freeze drying and spray drying) and fructose additions were studied for improvement of rice bran protein sensorial aroma characteristics. RESULTS: the most abundant amino acids in liquid HRPC (LH) were glutamic acid, arginine, aspartic acid and leucine, respectively...
February 19, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29456132/csf-lamp2-concentrations-are-decreased-in-female-parkinson-s-disease-patients-with-lrrk2-mutations
#2
Andrea C Klaver, Mary P Coffey, Jan O Aasly, David A Loeffler
Lysosome-associated membrane glycoprotein 2 (lamp2) plays critical roles in chaperone-mediated autophagy (CMA) and macroautophagy. Its isoform lamp2a is decreased in Parkinson's disease (PD) substantia nigra. Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene are the most known common cause of late-onset PD; although LRRK2 is thought to regulate macroautophagy, the influence of LRRK2 mutations on lamp2 concentrations in the CNS is unknown. To examine this issue we compared lamp2 levels in cerebrospinal fluid (CSF) between sporadic PD (sPD) patients (n = 31), LRRK2 PD patients (n = 20), and healthy control subjects with or without LRRK2 mutations (LRRK2 CTL = 30, CTL = 27)...
March 15, 2018: Brain Research
https://www.readbyqxmd.com/read/29455543/effect-of-leucine-on-nf-%C3%AE%C2%BAb-pathway-in-liver-regeneration-after-partial-hepatectomy-in-rats-as-determined-by-miniarray-analysis
#3
E Ceyhan, M Canbek, M Uyanoglu, A Ozen, B Durmus Ozen, B Berber, A Ozmen Yaylaci, L Resulzade
BACKGROUND: Although some amino acids are recognized to have favorable effects on the liver regeneration after partial hepatectomy (PH), molecular mechanisms underlying these effects are barely known. OBJECTIVE: Our study was aimed to investigate the effects of valine, glutamine, and leucine amino acids on PH-induced NF-κB signal pathway. The research team studied Leucine in a rat model in vivo. The study took place in the medical and surgical experimental research center at the Eskisehir Osmangazi University in Eskisehir, Turkey...
2018: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29454832/identification-and-functional-analysis-of-inhibitor-of-nf-%C3%AE%C2%BAb-kinase-ikk-from-scylla-paramamosain-the-first-evidence-of-three-ikks-in-crab-species-and-their-expression-profiles-under-biotic-and-abiotic-stresses
#4
Mei Jiang, Dan-Dan Tu, Wen-Bin Gu, Yi-Lian Zhou, Qi-Hui Zhu, Xiao-Ling Guo, Miao-An Shu
IKK (inhibitor of NF-κB kinase) is the core regulator for NF-κB (nuclear factor-κB) pathway against pathogenic invasion in vertebrates or invertebrates. However, the IKK from crab species has not yet been identified. In the present study, three full-length cDNA sequences of IKKs from mud crab Scylla paramamosain, designated as SpIKKβ, SpIKKε1 and SpIKKε2, were firstly cloned through RT-PCR and RACE methods. This is also the first report about the identification of two IKKε genes in mud crab and even in crustaceans...
February 15, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29454362/self-assembly-disassembly-hysteresis-of-nanoparticles-composed-of-marginally-soluble-short-elastin-like-polypeptides
#5
Markian S Bahniuk, Abdullah K Alshememry, Scott V Elgersma, Larry D Unsworth
BACKGROUND: Elastin-like polypeptides (ELPs) are a fascinating biomaterial that has undergone copious development for a variety of therapeutic applications including as a nanoscale drug delivery vehicle. A comprehensive understanding of ELP self-assembly is lacking and this knowledge gap impedes the advancement of ELP-based biomaterials into the clinical realm. The systematic examination of leucine-containing ELPs endeavors to expand existing knowledge about fundamental assembly-disassembly behaviours...
February 17, 2018: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/29454061/activation-of-nlrx1-mediated-autophagy-accelerates-the-ototoxic-potential-of-cisplatin-in-auditory-cells
#6
Haiyan Yin, Qianqian Yang, Zhixin Cao, Hongrui Li, Zhaoyan Yu, Guodong Zhang, Gaoying Sun, Rongjun Man, Haibo Wang, Jianfeng Li
To date, the mechanism (s) underlying the cisplatin-elicited ototoxicity has not been elucidated fully. Nucleotide-binding domain and leucine-rich-repeat-containing family member ×1 (NLRX1), a cytoplasmic pattern recognition receptor, is tightly related to mitochondrial function, reactive oxygen species (ROS) production, and autophagy. In this work, autophagy alteration, NLRX1 expression, ROS generation and cell injury were investigated correspondingly by immunofluorescence staining, western-blot, TEM, flow cytometry and MTT in HEI-OC1 cells of both NLRX1 overexpression and silencing in response to cisplatin stimulus...
February 14, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29453063/-1-h-nmr-based-metabolomics-reveals-interactive-effects-between-the-carrier-solvent-methanol-and-a-pharmaceutical-mixture-in-an-amphibian-developmental-bioassay-with-limnodynastes-peronii
#7
Steven D Melvin, Oliver A H Jones, Anthony R Carroll, Frederic D L Leusch
Organic carrier solvents are used in aquatic toxicity testing to improve chemical solubility and facilitate the exploration of dose-response relationships. Both water- and solvent-control groups are normally included in these scenarios to ensure that the solvent itself has no effect on the test organism, but this fails to consider possible interactive effects between carrier solvents and contaminants of interest. We explored this topic by exposing Limnodynastes peronii tadpoles to a mixture of common water-soluble pharmaceuticals (diclofenac, metformin and valproic acid) in the presence and absence of the carrier solvent methanol, according to standard developmental bioassay methodology...
February 10, 2018: Chemosphere
https://www.readbyqxmd.com/read/29452103/analysis-of-13-c-labeling-amino-acids-by-capillary-electrophoresis-high-resolution-mass-spectrometry-for-fluxomic-studies
#8
Sébastien Acket, Anthony Degournay, Mathilde Gosset, Franck Merlier, Manual Adrian Troncoso-Ponce, Brigitte Thomasset
In context of fluxomic studies, 13 C labeling analysis of amino acids are very important for solving the carbon flow calculation, because they are synthesized in various biosynthesis pathways and cellular compartments in plant cells. Traditionally, 13 C labeling analysis are performed using low resolution mass spectrometry detector by GC-MS. We developed a method using capillary electrophoresis-high resolution mass spectrometry without derivatization and with better accuracy assessment of labeling measurements comparing to classical GC-MS...
February 13, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29450946/down-regulation-of-lrig1-by-microrna-20a-modulates-gastric-cancer-multidrug-resistance
#9
Lin Zhou, Xiaowei Li, Fan Zhou, Zhi'an Jin, Di Chen, Pin Wang, Shu Zhang, Yuzheng Zhuge, Yulong Shang, Xiaoping Zou
Multidrug resistance (MDR) significantly restricts the clinical efficacy of gastric cancer (GC) chemotherapy, and it is critical to search novel targets to predict and overcome MDR. Leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) has been proved to be correlated with drug resistance in several cancers. The present study revealed that LRIG1 was overexpressed in chemo-sensitive GC tissues and decreased expression of LRIG1 predicted poor survival in GC patients. We observed that up-regulation of LRIG1 enhanced chemo-sensitivity in GC cells...
February 16, 2018: Cancer Science
https://www.readbyqxmd.com/read/29450546/a-plasma-metabolomic-signature-of-the-exfoliation-syndrome-involves-amino-acids-acylcarnitines-and-polyamines
#10
Stéphanie Leruez, Thomas Bresson, Juan M Chao de la Barca, Alexandre Marill, Grégoire de Saint Martin, Adrien Buisset, Jeanne Muller, Lydie Tessier, Cédric Gadras, Christophe Verny, Patrizia Amati-Bonneau, Guy Lenaers, Philippe Gohier, Dominique Bonneau, Gilles Simard, Dan Milea, Vincent Procaccio, Pascal Reynier
Purpose: To determine the plasma metabolomic signature of the exfoliative syndrome (XFS), the most common cause worldwide of secondary open-angle glaucoma. Methods: We performed a targeted metabolomic study, using the standardized p180 Biocrates Absolute IDQ p180 kit with a QTRAP 5500 mass spectrometer, to compare the metabolomic profiles of plasma from individuals with XFS (n = 16), and an age- and sex-matched control group with cataract (n = 18). Results: A total of 151 metabolites were detected correctly, 16 of which allowed for construction of an OPLS-DA model with a good predictive capability (Q2cum = 0...
February 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29450110/quantitative-analyses-of-the-equilibria-among-dna-complexes-of-a-blue-light-regulated-bzip-module-photozipper
#11
Yoichi Nakatani, Osamu Hisatomi
Aureochrome1 is a blue-light-receptor protein identified in a stramenopile alga, Vaucheria frigida . Photozipper (PZ) is an N-terminally truncated, monomeric, V. frigida aureochrome1 fragment containing a basic leucine zipper (bZIP) domain and a light-oxygen-voltage (LOV)-sensing domain. PZ dimerizes upon photoexcitation and consequently increases its affinity for the target sequence. In the present study, to understand the equilibria among DNA complexes of PZ, DNA binding by PZ and mutational variants was quantitatively investigated by electrophoretic-mobility-shift assay and fluorescence-correlation spectroscopy in the dark and light states...
2018: Biophysics and Physicobiology
https://www.readbyqxmd.com/read/29448508/-in-vivo-in-vitro-interaction-of-silver-nanoparticles-with-leucine-aminopeptidase-from-human-and-plasmodium-falciparum
#12
D Mnkandhla, J van Marwijk, H Hoppe, B S Wilhelmi, C G Whiteley
There is increasing requirement for the development of new drug protocols against malaria, a fatal disease caused by the lethal parasite Plasmodium falciparum. Leucine aminopeptidase (Pf LAP) of Plasmodium falciparum, is being pursued as a promising target for the discovery of novel antimalarials. The effects of silver nanoparticles (AgNPs) against P. falciparum leucine amino-peptidase (Pf LAP) and the human homolog (HsLAP) were compared. Pf LAP and HsLAP were expressed in Escherichia coli, and AgNPs (3-10 nm) characterized by ultra-violet spectroscopy and transmission electron microscopy...
February 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29445441/potential-new-cysteine-sparing-mutation-in-the-notch3-gene-in-a-patient-with-nonfamilial-cadasil-like-disease
#13
Adnan I Qureshi, Muhammad T Khan, Omer Naveed, Muhammad A Saleem
Background: Several different mutations have been reported in patients with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). We present a unique case with transversion not involving cysteine on neurogenic locus notch homolog protein 3 gene. Case description: We present a case of 65-year-old woman with new ischemic stroke resulting in right hemiparesis. She has previously suffered minor strokes at age 56, 58, and 60 years and migraine headaches between age 10 and 50 years...
December 2017: Journal of Vascular and Interventional Neurology
https://www.readbyqxmd.com/read/29444251/cultured-equine-satellite-cells-as-a-model-system-to-assess-leucine-stimulated-protein-synthesis-in-horse-muscle
#14
M L DeBoer, K M Martinson, M S Pampusch, A M Hansen, S M Wells, C Ward, M Hathaway
Leucine has been shown to stimulate the mammalian/mechanistic target of rapamycin (mTOR) signaling pathway which plays numerous key regulatory roles in cell growth, survival and metabolism including protein synthesis in a number of species. However, previous work with equine satellite cells has suggested distinct species differences in regards to physiological effects and the magnitude of responses to growth factors and regulators. Because there is limited research available regarding the role of leucine in regulating equine skeletal muscle protein synthesis, the objective of this study was to evaluate the effect of leucine on the mTOR signaling pathway in cultured equine satellite...
February 12, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29442259/lc-ms-based-serum-metabolomics-reveals-a-distinctive-signature-in-patients-with-rheumatoid-arthritis
#15
Ju Li, Nan Che, Lingxiao Xu, Qian Zhang, Qi Wang, Wenfeng Tan, Miaojia Zhang
Metabolomics has been applied to explore altered metabolite profiles in disease and identify unique metabolic signatures in recent years. We aim to characterize the metabolic profile of rheumatoid arthritis patients and explore its underlying pathological processes using metabolomics approach. Serum samples from 30 rheumatoid arthritis (RA) patients, 30 primary Sjogren's syndrome (pSS) patients, and 32 healthy controls (HC) were collected. The sample was analyzed by ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UPLC-HRMS)...
February 13, 2018: Clinical Rheumatology
https://www.readbyqxmd.com/read/29441955/pegylation-of-coenzyme-q-10-and-in-vitro-release-studies
#16
M Zacchigna, F Cateni, G Procida, D Zampieri, M G Mamolo
Methoxy polyethylene glycol conjugated with coenzyme Q10 (mPEG)-CoQ10 and analog adducts with amino acids as spacers were synthesized as a new drug delivery systems for CoQ10. Alanine and branched chain amino acids (valine, leucine and isoleucine) were conjugated to mPEG by an amide linkage and to CoQ10 by an ester bond. Recently, branched chain amino acids (BCAAs), which are released along with CoQ10, have received increasing attention as 'anti-fatigue' elements. FT-IR and 1H NMR spectroscopic analysis were useful to characterize the synthesized conjugates...
December 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29440269/the-mtdmi2-mtpub2-negative-feedback-loop-plays-a-role-in-nodulation-homeostasis
#17
Jiaxing Liu, Jie Deng, Fugui Zhu, Yuan Li, Zheng Lu, Peibin Qin, Tao Wang, Jiangli Dong
MtDMI2 is a leucine-rich repeat (LRR)-type receptor kinase required for the signal transduction in the symbiosis pathway. However, the mechanisms through which MtDMI2 participates in nodulation homeostasis are poorly understood. In this study, we identified MtPUB2, a new plant U-box (PUB)-type E3 ligase, and showed that it interacts with MtDMI2. The expression pattern of these proteins is similar in various tissues. MtPUB2-RNAi roots exhibit impaired infection threads, fewer nodules, and shorter primary root length compared with empty vector-transformed plants...
February 13, 2018: Plant Physiology
https://www.readbyqxmd.com/read/29439854/rapid-sensing-of-l-leucine-by-human-and-murine-hypothalamic-neurons-neurochemical-and-mechanistic-insights
#18
Nicholas Heeley, Peter Kirwan, Tamana Darwish, Marion Arnaud, Mark L Evans, Florian T Merkle, Frank Reimann, Fiona M Gribble, Clemence Blouet
OBJECTIVE: Dietary proteins are sensed by hypothalamic neurons and strongly influence multiple aspects of metabolic health, including appetite, weight gain, and adiposity. However, little is known about the mechanisms by which hypothalamic neural circuits controlling behavior and metabolism sense protein availability. The aim of this study is to characterize how neurons from the mediobasal hypothalamus respond to a signal of protein availability: the amino acid l-leucine. METHODS: We used primary cultures of post-weaning murine mediobasal hypothalamic neurons, hypothalamic neurons derived from human induced pluripotent stem cells, and calcium imaging to characterize rapid neuronal responses to physiological changes in extracellular l-Leucine concentration...
February 7, 2018: Molecular Metabolism
https://www.readbyqxmd.com/read/29439717/dysregulated-phosphorylation-of-rab-gtpases-by-lrrk2-induces-neurodegeneration
#19
Ga Ram Jeong, Eun-Hae Jang, Jae Ryul Bae, Soyoung Jun, Ho Chul Kang, Chi-Hu Park, Joo-Ho Shin, Yukio Yamamoto, Keiko Tanaka-Yamamoto, Valina L Dawson, Ted M Dawson, Eun-Mi Hur, Byoung Dae Lee
BACKGROUND: Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common cause of familial and sporadic Parkinson's disease (PD). Elevated kinase activity is associated with LRRK2 toxicity, but the substrates that mediate neurodegeneration remain poorly defined. Given the increasing evidence suggesting a role of LRRK2 in membrane and vesicle trafficking, here we systemically screened Rab GTPases, core regulators of vesicular dynamics, as potential substrates of LRRK2 and investigated the functional consequence of such phosphorylation in cells and in vivo...
February 13, 2018: Molecular Neurodegeneration
https://www.readbyqxmd.com/read/29437575/saturated-fatty-acids-undergo-intracellular-crystallization-and-activate-the-nlrp3-inflammasome-in-macrophages
#20
Tadayoshi Karasawa, Akira Kawashima, Fumitake Usui-Kawanishi, Sachiko Watanabe, Hiroaki Kimura, Ryo Kamata, Koumei Shirasuna, Yutaro Koyama, Ayana Sato-Tomita, Takashi Matsuzaka, Hiroshi Tomoda, Sam-Yong Park, Naoya Shibayama, Hitoshi Shimano, Tadashi Kasahara, Masafumi Takahashi
OBJECTIVE: Inflammation provoked by the imbalance of fatty acid composition, such as excess saturated fatty acids (SFAs), is implicated in the development of metabolic diseases. Recent investigations suggest the possible role of the NLRP3 (nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain containing 3) inflammasome, which regulates IL-1β (interleukin 1β) release and leads to inflammation, in this process. Therefore, we investigated the underlying mechanism by which SFAs trigger NLRP3 inflammasome activation...
February 8, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
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