keyword
MENU ▼
Read by QxMD icon Read
search

Gene testing

keyword
https://www.readbyqxmd.com/read/28823136/identification-of-polymorphisms-in-mitf-and-dct-genes-and-their-associations-with-plumage-colors-in-asian-duck-breeds
#1
Hasina Sultana, Dongwon Seo, Nu-Ri Choi, Md Shamsul Alam Bhuiyan, Seung Hwan Lee, Kang Nyeong Heo, Jun Heon Lee
Objective: The aim of this study was to investigate the effect of single nucleotide polymorphisms (SNPs) of the MITF and DCT genes on plumage coloration in Asian native duck breeds. MITF encodes a protein for microphthalmia-associated transcription factor, which regulates the development and function of melanocytes for pigmentation of skin, hair, and eyes. Among the tyrosinase-related family genes, dopachrome tautomerase (DCT) is a pigment cell-specific gene that plays important roles in the melanin synthesis pathway and the expression of skin, feather, and retina color...
August 16, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28822915/immunomodulatory-effects-of-m2000-%C3%AE-d-mannuronic-acid-on-tnf-%C3%AE-il-17-and-foxp3-gene-expression-in-patients-with-inflammatory-bowel-disease
#2
Hussaini Alhassan Mohammed, Ali Akbar Saboor-Yaraghi, Homayoun Vahedi, Mir Saeed Yekaninejad, Ghodratollah Panahi, Gholamreza Hemmasi, Mostafa Lakzaei, Abbas Mirshafiey
INTRODUCTION: Inflammatory bowel diseases (IBD) are immune-mediated disorders that result from an aberrant immunological response to the gut luminal antigen in genetically susceptible patients. IBD is categorized into two serotype, Crohn's diseases (CD) and ulcerative colitis (UC), both subtype are important cause of gastrointestinal diseases. The increasing rate of hospitalization, with the high economic burden experienced by the IBD patients, calls for more concerted research efforts to design a potent and affordable treatment option for the treatment of IBD...
August 17, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28822907/leaf-carbohydrates-influence-transcriptional-and-post-transcriptional-regulation-of-nocturnal-carboxylation-and-starch-degradation-in-the-facultative-cam-plant-mesembryanthemum-crystallinum
#3
Tahar Taybi, John C Cushman, Anne M Borland
Nocturnal degradation of transitory starch is a limiting factor for the optimal function of crassulacean acid metabolism and must be coordinated with phosphoenolypyruvate carboxylase (PEPC)-mediated CO2 uptake to optimise carbon gain over the diel cycle. The aim of this study was to test the hypothesis that nocturnal carboxylation is coordinated with starch degradation in CAM via a mechanism whereby the products of these pathways regulate diel transcript abundance and enzyme activities for both processes. To test this hypothesis, a starch and CAM-deficient mutant of Mesembryanthemum crystallinum was compared with wild type plants under well-watered and saline (CAM-inducing) conditions...
August 5, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28822901/morphological-and-molecular-differences-in-corpus-luteum-of-pregnant-sows-from-divergent-genetic-groups
#4
Karine A Costa, Walmir da Silva, Renata Veroneze, José C Montes, Lucas L Verardo, Margareth E Botelho, Márcio S Duarte, Mariana M Neves, Paulo S Lopes, José D Guimarães, Susana A Teixeira, Laene Alcantara, Simone E F Guimarães
Comprehending mechanisms controlling corpus luteum (CL) angiogenesis and apoptosis in pregnant sows is essential to understand the physiological role of these processes in CL function, progesterone production and consequently in conceptus development and prenatal mortality. CL from 54 sows from two genetic groups, a commercial line (COM) and the local Piau breed (LPB), were obtained for gene expression (n = 3 COM; n = 6 LPB), histological and protein analysis (n = 3 COM; n = 3 LPB), divided in six gestational ages (seven, 15, 30, 45, 60 and 90 days)...
July 29, 2017: Theriogenology
https://www.readbyqxmd.com/read/28822827/host-genes-regulate-transcription-of-sperm-introduced-hepatitis-b-virus-genes-in-embryo
#5
Ying Zhong, Dong-Ling Liu, Mohamed Morsi M Ahmed, Peng-Hao Li, Xiao-Ling Zhou, Qing-Dong Xie, Xiao-Qing Xu, Ting-Ting Han, Zhi-Wei Hou, Chen-Yao Zhong, Ji-Hua Huang, Fei Zeng, Tian-Hua Huang
Hepatitis B virus (HBV) can invade the male germline, and sperm-introduced HBV genes could be transcribed in embryo. This study was to explore whether viral gene transcription is regulated by host genes. Embryos were produced by in vitro fertilization of hamster oocytes with human sperm containing the HBV genome. Total RNA extracted from test and control embryos were subjected to SMART-PCR, SSH, microarray hybridization, sequencing and BLAST analysis. Twenty-nine sequences showing significant identity to five human gene families were identified, with CSH2, EIF4G2, PCBD2, PSG4 and TTN selected to represent target genes...
August 16, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28822785/next-generation-sequencing-based-detection-of-germline-copy-number-variations-in-brca1-brca2-validation-of-a-one-step-diagnostic-work-flow
#6
Ane Y Schmidt, Thomas V O Hansen, Lise B Ahlborn, Lars Jønson, Christina W Yde, Finn C Nielsen
Genetic testing of BRCA1/2 includes screening for single nucleotide variants and small indels as well as larger copy number variations (CNVs), primarily by Sanger sequencing and multiplex ligation-dependent probe amplification (MLPA). With the advent of next-generation sequencing (NGS), it has become feasible to provide CNV information as well as sequence data using a single platform. We report the use of NGS gene panel sequencing on the Illumina MiSeq platform and JSI SeqPilot SeqNext software to call germline CNVs in BRCA1 and BRCA2...
August 16, 2017: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/28822691/fatal-meningitis-in-swine-after-intrathecal-administration-of-adeno-associated-virus-expressing-syngeneic-interleukin-10
#7
Mark D Unger, Josef Pleticha, James E Collins, Anibal G Armien, Jennifer L Brazzell, Laura K Newman, Lukas F Heilmann, Jodi A Scholz, Timothy P Maus, Andreas S Beutler
Interleukin-10 (IL-10) delivered by intrathecal (i.t.) gene vectors is a candidate investigational new drug (IND) for several chronic neurological disorders such as neuropathic pain. We performed a preclinical safety study of IL-10. A syngeneic large animal model was used delivering porcine IL-10 (pIL-10) to the i.t. space in swine by adeno-associated virus serotype 8 (AAV8), a gene vector that was previously found to be nontoxic in the i.t. space. Unexpectedly, animals became ill, developing ataxia, seizures, and an inability to feed and drink, and required euthanasia...
August 1, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28822568/effects-of-lipopolysaccharide-on-the-proliferation-and-osteogenic-differentiation-of-stem-cells-from-the-apical-papilla
#8
Veera Lertchirakarn, Panuroot Aguilar
INTRODUCTION: Stem cells from the apical papilla (SCAPs) were suggested as the stem cell source in regenerative endodontic procedures. However, bone and/or cementum-like structure were observed in root canals. Lipopolysaccharide (LPS) in infected root canals might alter SCAPs' osteogenic differentiation pattern. The objectives of this study were to investigate the effects of LPS on SCAPs' proliferation and osteogenic differentiation. METHODS: The mesenchymal stem cell characteristics of SCAPs were confirmed...
August 16, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28822439/-multisystemic-smooth-muscle-dysfunction-syndrome-in-children-a-case-report-and-literature-review
#9
Y L Zhou, Y Y Zhang, B L Cheng, D Xu, L F Tang, Z M Chen
Objective: To analyze the clinical characteristics and diagnosis of multisystemic smooth muscle dysfunction syndrome(MSMDS). Method: Clinical data of a case diagnosed as MSMDS and hospitalized in our hospital in July 2016 was retrospectively analyzed. Literature search was performed at databases of PubMed, Wanfang, China National Knowledge Infrastructure and VIP with the key words "multisystemic smooth muscle dysfunction syndrome" "ACTA2" . The literature retrieval was confined from January 1980 to November 2016...
August 2, 2017: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/28822433/-molecular-biological-and-clinical-characteristics-of-respiratory-syncytial-virus-in-children-with-bronchiolitis
#10
T H Zhang, J Deng, Y Qian, R N Zhu, Y Sun, F Wang, R Tian, L Q Zhao
Objective: To investigate the clinical characteristics of respiratory syncytial virus(RSV)bronchiolitis and molecular biological characteristics of RSV in children in Beijing. Method: In a systematic retrospective study, 2 296 nasopharyngeal aspirates (NPA) were collected from children diagnosed with bronchiolitis from July 2006 to June 2016 for respiratory virus screening using direct immunofluorescence assay (DFA). For specimens positive for RSV, subgroup A or B was confirmed by real time RT-PCR and genotype of RSV was determined by amplifying the full G glycoprotein gene and sequencing...
August 2, 2017: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/28822308/the-acute-effects-of-ultraviolet-radiation-on-the-blood-transcriptome-are-independent-of-plasma-25ohd3
#11
Mariona Bustamante, Carles Hernandez-Ferrer, Yaris Sarria, Graham I Harrison, Lara Nonell, Wenjing Kang, Marc R Friedländer, Xavier Estivill, Juan R González, Mark Nieuwenhuijsen, Antony R Young
The molecular basis of many health outcomes attributed to solar ultraviolet radiation (UVR) is unknown. We tested the hypothesis that they may originate from transcriptional changes in blood cells. This was determined by assessing the effect of fluorescent solar simulated radiation (FSSR) on the transcriptional profile of peripheral blood pre- and 6h, 24h and 48h post-exposure in nine healthy volunteers. Expression of 20 genes was down-regulated and one was up-regulated at 6h after FSSR. All recovered to baseline expression at 24h or 48h...
August 16, 2017: Environmental Research
https://www.readbyqxmd.com/read/28822104/comparison-of-the-toxic-effects-of-quinolinic-acid-and-3-nitropropionic-acid-in-c-elegans-involvement-of-the-skn-1-pathway
#12
Ilan Kotlar, Aline Colonnello, María Fernanda Aguilera-González, Daiana Silva Avila, María Eduarda de Lima, Rodolfo García-Contreras, Alma Ortíz-Plata, Félix Alexandre Antunes Soares, Michael Aschner, Abel Santamaría
The tryptophan metabolite, quinolinic acid (QUIN), and the mitochondrial toxin 3-nitropropionic acid (3-NP) are two important tools for toxicological research commonly used in neurotoxic models of excitotoxicity, oxidative stress, energy depletion, and neuronal cell death in mammals. However, their toxic properties have yet to be explored in the nematode Caenorhabditis elegans (C. elegans) for the establishment of novel, simpler, complementary, alternative, and predictive neurotoxic model of mammalian neurotoxicity...
August 18, 2017: Neurotoxicity Research
https://www.readbyqxmd.com/read/28822036/filling-the-gaps-in-the-classification-of-the-digenea-carus-1863-systematic-position-of-the-proterodiplostomidae-dubois-1936-within-the-superfamily-diplostomoidea-poirier-1886-inferred-from-nuclear-and-mitochondrial-dna-sequences
#13
David Iván Hernández-Mena, Martín García-Varela, Gerardo Pérez-Ponce de León
The Diplostomida Olson, Cribb, Tkach, Bray & Littlewood, 2003 is the less diverse order of the two orders within the subclass Digenea Carus, 1863 and is currently classified into three superfamilies, i.e. Brachylaimoidea Joyeux & Foley, 1930, Diplostomoidea Poirier, 1886, and Schistosomatoidea Stiles & Hassall, 1898. Although the suprageneric-level relationships have been elucidated with the use of molecular markers, the lack of representation of some groups obscure the phylogenetic relationships among families, rendering the classification unstable...
August 18, 2017: Systematic Parasitology
https://www.readbyqxmd.com/read/28822029/gene-structure-and-comparative-and-phylogenetic-analyses-of-catla-catla-cyp1a-full-length-cdna-and-its-responsiveness-to-benzo-a-pyrene-and-copper-sulphate-at-early-developmental-stages
#14
Kavita Kumari, Gireesh-Babu Pathakota, Shivendra Kumar, Gopal Krishna
In the present study, full-length CYP1A cDNA from Catla catla (Catla) has been identified, and its real-time quantitative reverse transcription PCR (qRT-PCR) expression has been evaluated in different tissues, developmental stages (0, 3, 6, 12 and 24 h and 5, 7 and 9 days post-fertilization) and copper sulphate and benzo(a)pyrene (BaP)-treated 5-day post-fertilization (dpf) larvae (6 to 6.5 mm). Various structural, comparative and phylogenetic analyses of the deduced amino acid sequence revealed that the identified gene of Catla belongs to the CYP1A1 subfamily...
August 19, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28821995/expression-of-the-pol-gene-of-human-endogenous-retroviruses-herv-k-and-w-in-leukemia-patients
#15
Massimiliano Bergallo, Paola Montanari, Katia Mareschi, Chiara Merlino, Massimo Berger, Ilaria Bini, Valentina Daprà, Ilaria Galliano, Franca Fagioli
The human endogenous retroviruses (HERVs) are a family of endogenous retroviruses that integrated into the germ cell DNA of primates over 30 million years ago. HERV expression seems impaired in several diseases, ranging from autoimmune to neoplastic disorders. The purpose of this study was to evaluate the overall endogenous retroviral transcription profile in bone marrow (BM) samples. A total of 30 paediatric high-risk leukaemia patients (lymphoid and myeloid malignancies) were tested for HERVs virus gene expression...
August 18, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28821942/rapid-detection-of-serratia-fonticola-by-taqman-quantitative-real-time-pcr-using-primers-targeting-the-gyrb-gene
#16
Jing Hua Ruan, Wu Jun Wang, Ti Yin Zhang, Quan Yang Bai, Teng Zheng, Zhi Deng Zhang, Li Yun Wu, Yi Fan Huang, Dao Jin Yu
A gyrB gene is present in the majority of bacterial species, and encodes the ATPase domain of DNA gyraseB-subunit protein, which is essential for transcription and replication of bacteria. The gyrB gene exhibits higher nucleotide sequence variability than the 16S rDNA gene and thus could be more reliable in differentiating Serratia fonticola. A species-specific primer pair and probe were designed for quantitative real-time PCR detection of S. fonticola using gyrB as the target gene. Nine members of the Serratia family (representing nine Serratia species) were chosen to verify the specificity of the primers...
August 18, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28821923/ankg-hemizygous-mice-present-cognitive-impairment-and-elevated-anxiety-depressive-like-traits-associated-with-decreased-expression-of-gaba-receptors-and-postsynaptic-density-protein
#17
Cui Liu, Ling Zhang, Jie Wu, Xiaolong Sui, Yanfeng Xu, Lan Huang, Yunlin Han, Hua Zhu, Yanhong Li, Xiuping Sun, Chuan Qin
Recent genome-wide association studies (GWAS) of patient populations and genetic linkage assessments have demonstrated that the ankyrin-G (AnkG) gene is involved in neuropsychiatric disorders, including bipolar disorder, schizophrenia, and Alzheimer's disease, but it remains unclear how the genetic variants of AnkG contribute to neuropsychiatric disorders. Here, we generated AnkG hemizygous mice using the gene trapping approach. Homozygous AnkG was embryonically lethal. Western blotting and real-time polymerase chain reaction (qPCR) assessments of wild type (WT) and AnkG (+/-) mutant mice demonstrated a 50% reduction of ANKG levels, at the gene and protein levels, in AnkG hemizygous mice...
August 18, 2017: Experimental Brain Research. Experimentelle Hirnforschung. Expérimentation Cérébrale
https://www.readbyqxmd.com/read/28821819/dietary-factors-and-polymorphisms-in-vitamin-d-metabolism-genes-the-risk-and-prognosis-of-colorectal-cancer-in-northeast-china
#18
Chen Gong, Zhiping Long, Yanming Yu, Lin Zhu, Jingshen Tian, Shuo Li, Jing Li, Hongyuan Yu, Qiang Chi, Daxun Piao, Fan Wang, Yashuang Zhao, Binbin Cui
CYP24A1 and CYP27B1 are critical genes determining 1α,25(OH)2D3 concentration and impacting on carcinogenesis. A case-control study including 528 colorectal cancer (CRC) patients and 605 cancer-free controls and a follow-up study with 317 cases were conducted in northeast China. Genotypes were tested by TaqMan Genotyping Assays. Individuals carrying the GG genotype of CYP27B1 G > T (rs10877012) exhibited decreased CRC risk compared with those with the TT genotype (ORadjusted (ORadj) = 0.57, 95% Confidence Interval (CI) = 0...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821787/analysis-of-spatial-genetic-variation-reveals-genetic-divergence-among-populations-of-primula-veris-associated-to-contrasting-habitats
#19
Pablo Deschepper, Rein Brys, Miguel A Fortuna, Hans Jacquemyn
Genetic divergence by environment is a process whereby selection causes the formation of gene flow barriers between populations adapting to contrasting environments and is often considered to be the onset of speciation. Nevertheless, the extent to which genetic differentiation by environment on small spatial scales can be detected by means of neutral markers is still subject to debate. Previous research on the perennial herb Primula veris has shown that plants from grassland and forest habitats showed pronounced differences in phenology and flower morphology, suggesting limited gene flow between habitats...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821647/plasticity-beyond-v1-reinforcement-of-motion-perception-upon-binocular-central-retinal-lesions-in-adulthood
#20
Kalina Burnat, Tjing-Tjing Hu, Małgorzata Kossut, Ulf T Eysel, Lutgarde Arckens
Induction of a central retinal lesion in both eyes of adult mammals is a model for macular degeneration and leads to retinotopic map reorganization in the primary visual cortex (V1). Here we characterized the spatio-temporal dynamics of molecular activity levels in the central and peripheral representation of five higher order visual areas, V2/18, V3/19, V4/21a,V5/PMLS, area 7, and V1/17, in adult cats with central 10° retinal lesions (both sexes), by means of real-time PCR for the neuronal activity reporter gene zif268...
August 16, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
keyword
keyword
25650
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"