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Valeria Hansberg-Pastor, Aliesha González-Arenas, Ignacio Camacho-Arroyo
Many progesterone (P4) actions are mediated by its intracellular receptor (PR), which has two isoforms (PR-A and PR-B) differentially transcribed from separate promoters of a single gene. In glioblastomas, the most frequent and aggressive brain tumors, PR-B is the predominant isoform. In an in silico analysis we showed putative CCAAT/Enhancer Binding Protein (C/EBP) binding sites at PR-B promoter. We evaluated the role of C/EBPβ in PR-B expression regulation in glioblastoma cell lines, which expressed different ratios of PR and C/EBPβ isoforms (LAP1, LAP2, and LIP)...
September 20, 2016: Molecular and Cellular Endocrinology
Prerana Bhandari, Firoz Ahmad, Swarna Mandava, Bibhu Ranjan Das
BACKGROUND: Childhood acute lymphoblastic leukemia (ALL) is a heterogeneous genetic disease and its etiology remains poorly understood. Recent genome wide association and replication studies have highlighted specic polymorphisms contributing to childhood ALL predispositions mostly in European populations. It is unclear if these observations generalize to other populations with a lower incidence of ALL. The current case-control study evaluated variants in ARID5B (rs7089424, rs10821936), IKZF1 (rs4132601) and CEBPE (rs2239633) genes, which appear most significantly associated with risk of developing childhood B-lineage ALL...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
Masaki Mogi, Katsuhiko Kohara, Hirotomo Nakaoka, Harumi Kan-No, Kana Tsukuda, Xiao-Li Wang, Toshiyuki Chisaka, Hui-Yu Bai, Bao-Shuai Shan, Masayoshi Kukida, Jun Iwanami, Tetsuro Miki, Masatsugu Horiuchi
BACKGROUND: Sarcopenic obesity, age-related muscle loss, which is compensated by an increase in fat mass, impairs quality of life in elderly people. Although the increase in intramuscular fat is associated with decreased insulin sensitivity and increased metabolic risk factors, the origin of diabetes-associated intramuscular fat has not been elucidated. Here, we investigated intramuscular fat deposition using a muscle injury model in type 2 diabetic mice. METHODS: Male 8-week-old C57BL/6 and 8-week-old and 26-week-old KKAy underwent intramuscular injection of cardiotoxin (Ctx) (100 μL/10 μM) into the tibialis anterior (TA) muscles...
May 2016: Journal of Cachexia, Sarcopenia and Muscle
Amit J Sabnis, Christopher J Guerriero, Victor Olivas, Anin Sayana, Jonathan Shue, Jennifer Flanagan, Saurabh Asthana, Adrienne W Paton, James C Paton, Jason E Gestwicki, Peter Walter, Jonathan S Weissman, Peter Wipf, Jeffrey L Brodsky, Trever G Bivona
Cytosolic and organelle-based heat-shock protein (HSP) chaperones ensure proper folding and function of nascent and injured polypeptides to support cell growth. Under conditions of cellular stress, including oncogenic transformation, proteostasis components maintain homeostasis and prevent apoptosis. Although this cancer-relevant function has provided a rationale for therapeutically targeting proteostasis regulators (e.g., HSP90), cancer-subtype dependencies upon particular proteostasis components are relatively undefined...
August 9, 2016: Proceedings of the National Academy of Sciences of the United States of America
X M Guo, X P Liu, G B Chang, L Xu, Y L Bi, H Z Wang, Y Zhang, P F Zhu, Y Wu, G H Chen
NLRC5 plays an important role in the innate immunity and cellular immunity in many species, but the regulatory mechanism of NLRC5 expression in chickens remains unclear. In this study, a series of deletion fragments of the NLRC5 promoter region were constructed and dual-luciferase assay was performed. Then, we detected the SNP in the core region and its function. Important transcriptional regulatory elements were predicted and identified. Methylation of CpG islands was measured. The results revealed that the two core regions of -4372 to -3756 and -2925 to -2265 in the NLRC5 promoter were essential for NLRC5 mRNA expression in which a SNP (A/G), located at -2470, was found to have an effect on the transcriptional activity...
October 2016: Animal Genetics
Junpeng Liu, Weifeng Han, Lei Chen, Kanglai Tang
The aim of the present study was to assess the expression of sirtuin (Sirt)1 in tendon stem cells (TSCs) and to elucidate its association with osteogenic and adipogenic differentiation of TSCs. Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) and western blot analyses were performed to detect Sirt1 mRNA and protein levels in TSCs, respectively. TSCs were positive for Sirt1 expression, which was elevated by Sirt1 activator SRT1720 in a time- and concentration- dependent manner, and decreased by Sirt1 inhibitor EX527...
August 2016: Molecular Medicine Reports
Krishnakumar Malu, Rahul Garhwal, Margery G H Pelletier, Deepali Gotur, Stephanie Halene, Monika Zwerger, Zhong-Fa Yang, Alan G Rosmarin, Peter Gaines
Nuclear segmentation is a hallmark feature of mammalian neutrophil differentiation, but the mechanisms that control this process are poorly understood. Gene expression in maturing neutrophils requires combinatorial actions of lineage-restricted and more widely expressed transcriptional regulators. Examples include interactions of the widely expressed ETS transcription factor, GA-binding protein (GABP), with the relatively lineage-restricted E-twenty-six (ETS) factor, PU.1, and with CCAAT enhancer binding proteins, C/EBPα and C/EBPε...
August 1, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
Madara Kreile, Linda Piekuse, Dmitrijs Rots, Zane Dobele, Zhanna Kovalova, Baiba Lace
INTRODUCTION: Childhood acute lymphoblastic leukaemia (ALL) is a complex disease caused by a combination of genetic susceptibility and environmental exposure. Previous genome-wide association studies have reported several single nucleotide polymorphisms (SNPs) associated with the incidence of ALL. Several variations in genes encoding enzymes involved in carcinogenesis are suggested as being associated with an increased risk of ALL development. MATERIAL AND METHODS: We enrolled 77 paediatric ALL patients and 122 healthy controls, and in addition parental DNA was also available for 45 probands...
June 1, 2016: Archives of Medical Science: AMS
Ting-Yu Chang, Hsin-An Chen, Ching-Feng Chiu, Yi-Wen Chang, Tsang-Chih Kuo, Po-Chun Tseng, Weu Wang, Mien-Chie Hung, Jen-Liang Su
Paclitaxel is a standard-of-care chemotherapy for breast cancer, despite the increasing recognition of its poor effectiveness in the treatment of patients with advanced disease. Here, we report that adenovirus-type 5 E1A-mediated elevation of the miRNA-processing enzyme Dicer is sufficient to enhance paclitaxel sensitization and reduce cancer stem-like cell properties in this setting. Elevating Dicer expression increased levels of the AXL kinase targeting miRNA miR-494, thereby repressing AXL expression to increase paclitaxel sensitivity...
July 1, 2016: Cancer Research
Rebekah H Nagler, Cabral A Bigman, Shoba Ramanadhan, Divya Ramamurthi, K Viswanath
BACKGROUND: Americans remain under-informed about cancer and other health disparities and the social determinants of health (SDH). The news media may be contributing to this knowledge deficit, whether by discussing these issues narrowly or ignoring them altogether. Because local media are particularly important in influencing public opinion and support for public policies, this study examines the prevalence and framing of disparities/SDH in local mainstream and ethnic print news. METHODS: We conducted a multi-method content analysis of local mainstream (English language) and ethnic (Spanish language) print news in two lower income cities in New England with substantial racial/ethnic minority populations...
April 2016: Cancer Epidemiology, Biomarkers & Prevention
Electra D Paskett, Jessica L Krok-Schoen, Michael L Pennell, Cathy M Tatum, Paul L Reiter, Juan Peng, Brittany M Bernardo, Rory C Weier, Morgan S Richardson, Mira L Katz
BACKGROUND: Uptake of the human papillomavirus (HPV) vaccine is low in Appalachian Ohio and areas with high cervical cancer rates. METHODS: We conducted a group-randomized trial among 12 counties in Appalachian Ohio randomized to receive either an HPV vaccine (intervention counties) or influenza vaccine (comparison counties) multilevel intervention (MLI). Parents (n = 337) who had a daughter aged 9 to 17 years who had not received the HPV vaccine were recruited from commercial lists...
April 2016: Cancer Epidemiology, Biomarkers & Prevention
Gloria D Coronado, Shirley A A Beresford, Dale McLerran, Ricardo Jimenez, Donald L Patrick, India Ornelas, Sonia Bishop, John R Scheel, Beti Thompson
BACKGROUND: Breast cancer is the most commonly diagnosed cancer in women in the United States, and Latinas have relatively low rates of screening participation. The Multilevel Intervention to Increase Latina Participation in Mammography Screening study (¡Fortaleza Latina!) sought to assess the efficacy of a clinic- and patient-level program to increase breast cancer screening among Latinas in Western Washington who seek care at a safety net health center. METHODS: The study enrolled 536 Latinas ages 42 to 74 who had a primary care clinic visit in the previous 5 years and had not obtained a mammogram in the previous 2 years...
April 2016: Cancer Epidemiology, Biomarkers & Prevention
Damali N Martin, Tram Kim Lam, Katy Brignole, Kimlin T Ashing, William J Blot, Linda Burhansstipanov, Jarvis T Chen, Mark Dignan, Scarlett Lin Gomez, Maria Elena Martinez, Alicia Matthews, Julie R Palmer, Eliseo J Perez-Stable, Mario Schootman, Hugo Vilchis, Alexander Vu, Shobha Srinivasan
Medically underserved populations in the United States continue to experience higher cancer burdens of incidence, mortality, and other cancer-related outcomes. It is imperative to understand how health inequities experienced by diverse population groups may contribute to our increasing unequal cancer burdens and disparate outcomes. The National Cancer Institute convened a diverse group of scientists to discuss research challenges and opportunities for cancer epidemiology in medically underserved and understudied populations...
April 2016: Cancer Epidemiology, Biomarkers & Prevention
Hanene Gharbi, Islem Ben Hassine, Ismail Soltani, Ines Safra, Slah Ouerhani, Hind Bel Haj Othmen, Mouheb Teber, Ahlem Farah, Hassiba Amouri, Nourel Houda Toumi, Salima Abdennebi, Salem Abbes, Samia Menif
Recent genome-wide association studies (GWAS) focusing on pediatric acute lymphoblastic leukemia (ALL), the most common malignancy in children younger than 15 years old, have found evidence that single-nucleotide polymorphisms (SNPs) in IKZF1 (7p12.2), ARID5B (10q21.2), CDKN2A (9p21.3), and CEBPE (14q11.2) are strongly associated to the risk of developing pediatric ALL. These studies have been conducted in European and Thai populations, and it is unclear whether these observations generalize to other populations with a lower incidence of pediatric ALL...
April 2016: Pediatric Hematology and Oncology
Gaëlle Palierne, Aurélie Fabre, Romain Solinhac, Christine Le Péron, Stéphane Avner, Françoise Lenfant, Coralie Fontaine, Gilles Salbert, Gilles Flouriot, Jean-François Arnal, Raphaël Métivier
Transcriptional regulation by the estrogen receptor-α (ER) has been investigated mainly in breast cancer cell lines, but estrogens such as 17β-estradiol (E2) exert numerous extrareproductive effects, particularly in the liver, where E2 exhibits both protective metabolic and deleterious thrombotic actions. To analyze the direct and early transcriptional effects of estrogens in the liver, we determined the E2-sensitive transcriptome and ER cistrome in mice after acute administration of E2 or placebo. These analyses revealed the early induction of genes involved in lipid metabolism, which fits with the crucial role of ER in the prevention of liver steatosis...
July 2016: Molecular Endocrinology
Juan Tornin, Lucia Martinez-Cruzado, Laura Santos, Aida Rodriguez, Luz-Elena Núñez, Patricia Oro, Maria Ana Hermosilla, Eva Allonca, Maria Teresa Fernández-García, Aurora Astudillo, Carlos Suarez, Francisco Morís, Rene Rodriguez
Tumor initiating cells (TICs), responsible for tumor initiation, and cancer stem cells (CSCs), responsible for tumor expansion and propagation, are often resistant to chemotherapeutic agents. To find therapeutic targets against sarcoma initiating and propagating cells we used models of myxoid liposarcoma (MLS) and undifferentiated pleomorphic sarcoma (UPS) developed from human mesenchymal stromal/stem cells (hMSCs), which constitute the most likely cell-of-origin for sarcoma. We found that SP1-mediated transcription was among the most significantly altered signaling...
May 24, 2016: Oncotarget
Xinzheng Jia, Huiran Lin, Bahareldin Ali Abdalla, Qinghua Nie
miRNAs have been widely investigated in terms of cell proliferation and differentiation. However, little is known about their effects on bird growth. Here we characterized the promoter of miR-206 in chicken and found that the preferable promoter was located in 1200 bp upstream of pri-miR-206. In this region, many key transcription factors, including MyoD, c-Myb, CEBPα/β, AP-4, RAP1, Brn2, GATA-1/2/3, E47, Sn, upstream stimulatory factor (USF) and CdxA, were predicted to bind and interact with miR-206 promoter...
2016: International Journal of Molecular Sciences
Leonardo M R Ferreira, Torsten B Meissner, Tarjei S Mikkelsen, William Mallard, Charles W O'Donnell, Tamara Tilburgs, Hannah A B Gomes, Raymond Camahort, Richard I Sherwood, David K Gifford, John L Rinn, Chad A Cowan, Jack L Strominger
HLA-G, a nonclassical HLA molecule uniquely expressed in the placenta, is a central component of fetus-induced immune tolerance during pregnancy. The tissue-specific expression of HLA-G, however, remains poorly understood. Here, systematic interrogation of the HLA-G locus using massively parallel reporter assay (MPRA) uncovered a previously unidentified cis-regulatory element 12 kb upstream of HLA-G with enhancer activity, Enhancer L Strikingly, clustered regularly-interspaced short palindromic repeats (CRISPR)/Cas9-mediated deletion of this enhancer resulted in ablation of HLA-G expression in JEG3 cells and in primary human trophoblasts isolated from placenta...
May 10, 2016: Proceedings of the National Academy of Sciences of the United States of America
Shaikh M Rahman, Karalee C Baquero, Mahua Choudhury, Rachel C Janssen, Becky A de la Houssaye, Ming Sun, Shinobu Miyazaki-Anzai, Shu Wang, Naima Moustaid-Moussa, Makoto Miyazaki, Jacob E Friedman
BACKGROUND AND OBJECTIVE: Atherosclerosis is both a chronic inflammatory disease and a lipid metabolism disorder. C/EBPβ is well documented for its role in the development of hematopoietic cells and integration of lipid metabolism. However, C/EBPβ's role in atherosclerotic progression has not been examined. We assessed the impact of hematopoietic CEBPβ deletion in ApoE(-/-) mice on hyperlipidemia, inflammatory responses and lesion formation in the aorta. METHODS AND RESULTS: ApoE(-/-) mice were reconstituted with bone marrow cells derived from either WT or C/EBPβ(-/-) mice and placed on low fat or high fat/high cholesterol diet for 11 weeks...
July 2016: Atherosclerosis
Enikö Tóth, Dániel Beyer, Barbara Zsebik, György Vereb, Lili Takács
OBJECTIVES: Corneal blindness due to limbal stem-cell deficiency can be treated by transplantation of cultivated limbal epithelial stem cells (LESCs). We examined LESC cultivation on a contact lens (CL) carrier. Our goal was to optimize explant affixation and assess the possible benefit of 3T3 feeder cells. METHODS: Human cadaver limbal and conjunctival explants were allowed to attach to CLs under the airflow of the laminar box (dried group) or affixed on CLs using suturing (sutured group) or tissue adhesives (glued group), then cultivated with or without 3T3 feeder cells...
April 6, 2016: Eye & Contact Lens
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