keyword
MENU ▼
Read by QxMD icon Read
search

Tagging and capture

keyword
https://www.readbyqxmd.com/read/28429065/rapid-electrochemical-quantification-of-salmonella-pullorum-and-salmonella-gallinarum-based-on-glucose-oxidase-and-antibody-modified-silica-nanoparticles
#1
Yiheng Luo, Wenchao Dou, Guangying Zhao
In this article, a facile and sensitive electrochemical method for quantification of Salmonella Pullorum and Salmonella Gallinarum (S. Pullorum and S. Gallinarum) was established by monitoring glucose consumption with a personal glucose meter (PGM). Antibody-functionalized magnetic nanoparticles (IgG-MNPs) were used to capture and enrich S. Pullorum and S. Gallinarum, and IgG-MNPs-S. Pullorum and IgG-MNPs-S. Gallinarum complexes were magnetically separated from a sample using a permanent magnet. The trace tag was prepared by loading polyclonal antibodies and high-content glucose oxidase on amino-functionalized silica nanoparticles (IgG-SiNPs-GOx)...
April 21, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28426149/click-chemistry-mediated-rapid-microbubble-catching-for-acute-thrombus-ultrasound-molecular-imaging
#2
Tuantuan Wang, Chuxiao Yuan, Bingyang Dai, Yang Liu, Mingxi Li, Zhenqiang Feng, Qing Jiang, Zhihong Xu, Ningwei Zhao, Ning Gu, Fang Yang
Bioorthogonal coupling chemistry has been studied as one significant advantage for molecular imaging as it offers rapid, efficient, and strong binding, which may also benefit in stability, production, and chemical conjugation. The inverse-electron-demand Diels-Alder reaction between s-tetrazine and trans-cyclooctene (TCO) is an example of a highly selective and rapid bioorthogonal coupling reaction to be used successfully to prepare targeted molecular imaging probes. Herein, based on a two-step pretargeting bioorthogonal chemistry, we report a fast and reliable highly sensitive approach to achieve activated-platelet-specific CD62p targeted thrombus ultrasound molecular imaging...
April 20, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28424589/the-calcium-channel-c-terminal-and-synaptic-vesicle-tethering-analysis-by-immuno-nanogold-localization
#3
Robert H C Chen, Qi Li, Christine A Snidal, Sabiha R Gardezi, Elise F Stanley
At chemical synapses the incoming action potential triggers the influx of Ca(2+) through voltage-sensitive calcium channels (CaVs, typically CaV2.1 and 2.2) and the ions binds to sensors associated with docked, transmitter filled synaptic vesicles (SVs), triggering their fusion and discharge. The CaVs and docked SVs are located within the active zone (AZ) region of the synapse which faces a corresponding neurotransmitter receptor-rich region on the post-synaptic cell. Evidence that the fusion of a SV can be gated by Ca(2+) influx through a single CaV suggests that the channel and docked vesicle are linked by one or more molecular tethers (Stanley, 1993)...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28422163/distorted-immunodominance-by-linker-sequences-or-other-epitopes-from-a-second-protein-antigen-during-antigen-processing
#4
AeRyon Kim, Tatiana N Boronina, Robert N Cole, Erika Darrah, Scheherazade Sadegh-Nasseri
The immune system focuses on and responds to very few representative immunodominant epitopes from pathogenic insults. However, due to the complexity of the antigen processing, understanding the parameters that lead to immunodominance has proved difficult. In an attempt to uncover the determinants of immunodominance among several dominant epitopes, we utilized a cell free antigen processing system and allowed the system to identify the hierarchies among potential determinants. We then tested the results in vivo; in mice and in human...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28412658/cds-mn-quantum-dot-functionalized-g-c3n4-nanohybrids-as-signal-generation-tags-for-photoelectrochemical-immunoassay-of-prostate-specific-antigen-coupling-dnazyme-concatamer-with-enzymatic-biocatalytic-precipitation
#5
Kangyao Zhang, Shuzhen Lv, Zhenzhen Lin, Dianping Tang
A new photoelectrochemical (PEC) immunosensor based on Mn-doped CdS quantum dots (CdS:Mn QDs) on g-C3N4 nanosheets was developed for the sensitive detection of prostate specific antibody (PSA) in biological fluids. The signal derived from the as-synthesized Cd:Mn QDs-functionalized g-C3N4 nanohybrids via a hydrothermal method and was amplified through DNAzyme concatamers on gold nanoparticles accompanying enzymatic biocatalytic precipitation. Experimental results by UV-vis absorption spectra and photoluminescence revealed that CdS:Mn QDs/g-C3N4 nanohybrids exhibited higher photocurrent than those of CdS:Mn QDs and g-C3N4 alone...
April 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28412657/potential-resolved-in-electrode-type-electrochemiluminescence-immunoassay-based-on-functionalized-g-c3n4-nanosheet-and-ru-nh2-for-simultaneous-determination-of-dual-targets
#6
Zhiyong Guo, Lin Wu, Yufang Hu, Sui Wang, Xing Li
Here, a novel potential-resolved "in-electrode" type electrochemiluminescence (ECL) immunosensor was fabricated based on two different types of luminant Ru-NH2 and AuNPs/g-C3N4 to realize simultaneous detection of dual targets. In this strategy, anti-CA1251 and anti-SCCA1 were immobilized on bare gold electrode as capture probes, which could catch the two corresponding target CA125 and SCCA, and the immobilization of the signal tags was allowed via the interaction between antigen and antibody. In this process, (Ru&anti-CA1252)@GO and anti-SCCA2-AuNPs/g-C3N4 could exhibit two strong and stable ECL emissions at 1...
March 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28411820/thrombin-aptasensor-enabled-by-pt-nanoparticles-functionalized-co-based-metal-organic-frameworks-assisted-electrochemical-signal-amplification
#7
Yang Yang, Zhehan Yang, Jiajia Lv, Ruo Yuan, Yaqin Chai
In this work, a Pt nanoparticles-functionalized Co-based metal organic frameworks (PtNPs@Co(II)MOFs@PtNPs) was synthesized and applied in electrochemical aptasensor for thrombin (TB) detection. First, the Co(II)MOFs@PtNPs were prepared via the mixed solvothermal method, which consists of inner Pt nanoparticles (PtNPs) encapsulated by aminofunctionalized Co(II)MOFs materials. Following that, additional PtNPs were adsorbed on the surface of Co(II)MOFs@PtNPs, resulting in the formation of PtNPs@Co(II)MOFs@PtNPs nanocomposite...
July 1, 2017: Talanta
https://www.readbyqxmd.com/read/28411532/one-pot-loading-high-content-thionine-on-polydopamine-functionalized-mesoporous-silica-nanosphere-for-ultrasensitive-electrochemical-immunoassay
#8
Guosong Lai, Min Zheng, Wenjie Hu, Aimin Yu
A thionine (TH)-doped mesoporous silica nanosphere (MSN)/polydopamine (PDA) nanocomposite was synthesized for developing a new signal transduction strategy of electrochemical immunoassay. This nanocomposite was synthesized through one-pot loading of TH and in situ formation of PDA-coating on MSN. After antibody labeling, the obtained nanoprobe was used for the signal tracing of sandwich immunoassay at a magnetic bead-assay platform. Based on the specific capture of the MSN-TH/PDA nanoprobes through sandwich immunoreaction to form a magnetic immunocomplex and the following treatment of the immunocomplex with a NaOH solution, the PDA film coated on MSN was destroyed to release TH tags from the nanoprobe...
April 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28411238/characterization-of-protein-complexes-of-the-endoplasmic-reticulum-associated-degradation-e3-ubiquitin-ligase-hrd1
#9
Jiwon Hwang, Christopher P Walczak, Thomas A Shaler, James A Olzmann, Lichao Zhang, Joshua E Elias, Ron R Kopito
Hrd1 is the core structural component of a large ER membrane-embedded protein complex that coordinates the destruction of folding-defective proteins in the early secretory pathway. Defining the composition, dynamics, and ultimately, the structure of the Hrd1 complex is a crucial step in understanding the molecular basis of glycoprotein quality control, but has been hampered by the lack of suitable techniques to interrogate this complex under native conditions. In this study we used genome editing to generate clonal HEK293 (Hrd1...
April 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28396838/thermo-kinetic-analysis-space-expansion-for-cyclophilin-ligand-interactions-identification-of-a-new-nonpeptide-inhibitor-using-biacore%C3%A2-t200
#10
Martin A Wear, Matthew W Nowicki, Elizabeth A Blackburn, Iain W McNae, Malcolm D Walkinshaw
We have established a refined methodology for generating surface plasmon resonance sensor surfaces of recombinant his-tagged human cyclophilin-A. Our orientation-specific stabilisation approach captures his-tagged protein under 'physiological conditions' (150 mm NaCl, pH 7.5) and covalently stabilises it on Ni(2+)-nitrilotriacetic acid surfaces, very briefly activated for primary amine-coupling reactions, producing very stable and active surfaces (≥ 95% specific activity) of cyclophilin-A. Variation in protein concentration with the same contact time allows straightforward generation of variable density surfaces, with essentially no loss of activity, making the protocol easily adaptable for studying numerous interactions; from very small fragments, ~ 100 Da, to large protein ligands...
April 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28395772/an-electrochemical-aptasensor-for-multiplex-antibiotics-detection-using-y-shaped-dna-based-metal-ions-encoded-probes-with-nmof-substrate-and-csrp-target-triggered-amplification-strategy
#11
Meng Chen, Ning Gan, Tianhua Li, Ye Wang, Qing Xu, Yinji Chen
An effective electrochemical aptasensor has been developed for the detection of multiplex antibiotics using Y-shaped DNA probes. These probes-based metal ions encoded the nanoscale metal-organic frameworks (NMOF) as a substrate, and circular strand-replacement DNA polymerization (CSRP) target triggered the amplification strategy. The Y-DNA probes (Y-DNA) were assembled using an assisted DNA probe (assisted DNA labeled with magnetic gold nanoparticles) which can hybridize to the captured DNA probe (consisting of aptamer and primer recognition region), and signal tags (NMOF encapsulating signal DNAs and different metal ions such as Pb(2+) or Cd(2+))...
May 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28394205/a-three-dimensional-computational-human-head-model-that-captures-live-human-brain-dynamics
#12
Shailesh Ganpule, Nitin P Daphalapurkar, Kaliat T Ramesh, Andrew K Knutsen, Dzung L Pham, Philip V Bayly, Jerry L Prince
Diffuse axonal injury (DAI) is a debilitating consequence of traumatic brain injury (TBI) attributed to abnormal stretching of axons caused by blunt head trauma or acceleration of the head. We developed an anatomically accurate, subject-specific, three-dimensional (3D) computational model of the human brain, and used it to study the dynamic deformations in the substructures of the brain when the head is subjected to rotational accelerations. The computational head models use anatomy and morphology of the white matter fibers obtained using MRI...
April 10, 2017: Journal of Neurotrauma
https://www.readbyqxmd.com/read/28393516/ultrasensitive-electrochemical-detection-of-glycoprotein-based-on-boronate-affinity-sandwich-assay-and-signal-amplification-with-functionalized-sio2-au-nanocomposites
#13
Min You, Shuai Yang, Wanxin Tang, Fan Zhang, Pin-Gang He
Herein we propose a multiple signal amplification strategy designed for ultrasensitive electrochemical detection of glycoproteins. This approach introduces a new type of boronate-affinity sandwich assay (BASA), which was fabricated by using gold nanoparticles combined with reduced graphene oxide (AuNPs-GO) to modify sensing surface for accelerating electron transfer, the composite of molecularly imprinted polymer (MIP) including 4-vinylphenylboronic acid (VPBA) for specific capturing glycoproteins, and SiO2 nanoparticles carried gold nanoparticles (SiO2@Au) labeled with 6-ferrocenylhexanethiol (FcHT) and 4-mercaptophenylboronic acid (MPBA) (SiO2@Au/FcHT/MPBA) as tracing tag for binding glycoprotein and generating electrochemical signal...
April 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28393515/aptamer-protein-proximity-binding-triggered-molecular-machine-for-amplified-electrochemical-sensing-of-thrombin
#14
Jianmei Yang, Baoting Dou, Ruo Yuan, Yun Xiang
The development of convenient and sensitive methods without involving any enzymes or complex nanomaterials for the monitoring of proteins is of great significance in disease diagnostics. In this work, we describe the validation of a new aptamer/protein proximity binding-triggered molecular machinery amplification strategy for sensitive electrochemical assay of thrombin in complex serum samples. The sensing interface is prepared by self-assembly of three-stranded DNA complexes on the gold electrode. The association of two distinct functional aptamers with different sites of thrombin triggers proximity binding-induced displacement of one of the short single-stranded DNAs (ssDNAs) from the surface-immobilized three-stranded DNA complexes, exposing a prelocked toehold domain to hybridize with a methylene blue (MB)-tagged fuel ssDNA strand (MB-DNA)...
April 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28386422/horizontal-and-vertical-movements-of-caribbean-reef-sharks-carcharhinus-perezi-conservation-implications-of-limited-migration-in-a-marine-sanctuary
#15
Oliver N Shipley, Lucy A Howey, Emily R Tolentino, Lance K B Jordan, Jonathan L W Ruppert, Edward J Brooks
Despite the ecological and economic importance of the Caribbean reef shark (Carcharhinus perezi), little data exist regarding the movements and habitat use of this predator across its range. We deployed 11 pop-up satellite archival tags on Caribbean reef sharks captured in the northeast Exuma Sound, The Bahamas, to assess their horizontal and vertical movements throughout the water column. Sharks showed high site fidelity to The Bahamas suggesting Bahamian subpopulations remain protected within the Bahamian Shark Sanctuary...
February 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28372727/highly-sensitive-electrochemical-immunoassay-integrated-with-polymeric-nanocomposites-and-enhanced-sio2-au-core-shell-nanobioprobes-for-sirt1-determination
#16
Yarui An, Guanyang Zhu, Wenji Bi, Linlin Lu, Chongchong Feng, Zhiai Xu, Wen Zhang
An ultra-highly sensitive electrochemical immunosensor for SirT1 (a key protein in age-related diseases) evaluation has been designed, employing polymeric nanocomposites as sensing platform and core-shell SiO2@Au to immobilize HRP-Ab2 as nanobioprobes. The approach includes chemical synthesis of PAMAM-Au-MWCNT nanocomposites (PNCs) with abundant PAMAM-Au nanoparticles immobilized on the MWCNT matrix, and biochemically synthesis of nanobioprobes with highly dispersed SiO2@Au tracing tags for successive efficient load functionalized enzyme-antibodies (HRP-Ab2)...
May 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28358394/long-read-chia-pet-for-base-pair-resolution-mapping-of-haplotype-specific-chromatin-interactions
#17
Xingwang Li, Oscar Junhong Luo, Ping Wang, Meizhen Zheng, Danjuan Wang, Emaly Piecuch, Jacqueline Jufen Zhu, Simon Zhongyuan Tian, Zhonghui Tang, Guoliang Li, Yijun Ruan
Chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) is a robust method for capturing genome-wide chromatin interactions. Unlike other 3C-based methods, it includes a chromatin immunoprecipitation (ChIP) step that enriches for interactions mediated by specific target proteins. This unique feature allows ChIA-PET to provide the functional specificity and higher resolution needed to detect chromatin interactions, which chromosome conformation capture (3C)/Hi-C approaches have not achieved. The original ChIA-PET protocol generates short paired-end tags (2 × 20 base pairs (bp)) to detect two genomic loci that are far apart on linear chromosomes but are in spatial proximity in the folded genome...
May 2017: Nature Protocols
https://www.readbyqxmd.com/read/28340759/a-label-free-and-universal-platform-for-antibiotics-detection-based-on-microchip-electrophoresis-using-aptamer-probes
#18
Lingying Zhou, Ning Gan, You Zhou, Tianhua Li, Yuting Cao, Yinji Chen
A novel label-free, universal, and high throughput aptasensor was developed based on a microchip electrophoresis (MCE) platform for automatic detection of antibiotic residues in food. Firstly, chloramphenicol (CAP) was employed as a model to be captured by its aptamer probe (Apt). Then, the partial complementary oligonucleotide of CAP's aptamer (C-DNA) was introduced into the reaction system. Because the Apt-CAP complex can't further hybrid with free C-DNA, the amount of hybrid Apt-C-DNA double strand DNA (dsDNA) was less than that without adding the target...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28339747/deep-learning-for-pharmacovigilance-recurrent-neural-network-architectures-for-labeling-adverse-drug-reactions-in-twitter-posts
#19
Anne Cocos, Alexander G Fiks, Aaron J Masino
Objective: Social media is an important pharmacovigilance data source for adverse drug reaction (ADR) identification. Human review of social media data is infeasible due to data quantity, thus natural language processing techniques are necessary. Social media includes informal vocabulary and irregular grammar, which challenge natural language processing methods. Our objective is to develop a scalable, deep-learning approach that exceeds state-of-the-art ADR detection performance in social media...
February 22, 2017: Journal of the American Medical Informatics Association: JAMIA
https://www.readbyqxmd.com/read/28335964/highly-sensitive-colorimetric-immunosensor-for-influenza-virus-h5n1-based-on-enzyme-encapsulated-liposome
#20
Cuiying Lin, Yajuan Guo, Mengmeng Zhao, Mi Sun, Fang Luo, Longhua Guo, Bin Qiu, Zhenyu Lin, Guonan Chen
Development of simple but sensitive biosensor for influenza detection is highly important in immediate and effective clinical treatment. In this study, a sensitive colorimetric immunosensor which combines the advantages of high selectivity of immunoassay and simplicity of colorimetric detection has been developed to detect influenza virus H5N1 based on enzyme-encapsulated liposome. Biotin-tagged liposome encapsulated with large amount of horseradish peroxidase (HRP) was firstly synthesized. In the presence of H5N1, H5N1 co-bound with the capture antibody and the biotinylated detection antibody to form sandwich immunocomplex...
April 22, 2017: Analytica Chimica Acta
keyword
keyword
25559
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"