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https://www.readbyqxmd.com/read/28640955/in-solution-antibody-harvesting-with-a-plant-produced-hydrophobin-protein-a-fusion
#1
Katri Kurppa, Lauri Reuter, Anneli Ritala, Markus Linder, Jussi Joensuu
Purification is a bottleneck and a major cost factor in the production of antibodies. We set out to engineer a bi-functional fusion protein from two building blocks, Protein A and a hydrophobin, aiming at low-cost and scalable antibody capturing in solutions. Immunoglobulin-binding Protein A is widely used in affinity-based purification. The hydrophobin fusion tag, on the other hand, has been shown to enable purification by two-phase separation. Protein A was fused to two different hydrophobin tags, HFBI or II, and expressed transiently in Nicotiana benthamiana...
June 22, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28640899/normative-3d-opto-electronic-stereo-photogrammetric-posture-and-spine-morphology-data-in-young-healthy-adult-population
#2
Moreno D'Amico, Edyta Kinel, Piero Roncoletta
Observational cross-sectional study. The current study aims to yield normative data: i.e., the physiological standard for 30 selected quantitative 3D parameters that accurately capture and describe a full-skeleton, upright-standing attitude. Specific and exclusive consideration was given to three distinct categories: postural, spine morphology and pelvic parameters. To capture such 3D parameters, the authors selected a non-ionising 3D opto-electronic stereo-photogrammetric approach. This required the identification and measurement of 27 body landmarks, each specifically tagged with a skin marker...
2017: PloS One
https://www.readbyqxmd.com/read/28632533/investigation-of-two-prototypes-of-novel-noncontact-technologies-for-automated-real-time-capture-of-incremental-drug-administration-data-from-syringes
#3
Benjamin Eagle, David J Williams, John Dingley
BACKGROUND: An ideal electronic anesthesia recording system would be capable of not only recording physiological data but also injectable drug doses given, including those given incrementally from one syringe, without recourse to manual data entry. We compared 2 prototype devices which wirelessly recognized individual syringes and measured changes in their plunger positions via 2 different optical noncontact means, allowing calculation of incremental drug doses given. METHODS: Both devices incorporated a radio-frequency identification reader, which wirelessly read a unique code from a radio-frequency identification tag within syringe drug labels...
June 17, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28623569/estimating-the-population-size-of-female-sex-worker-population-in-tehran-iran-application-of-direct-capture-recapture-method
#4
Manoochehr Karami, Salman Khazaei, Jalal Poorolajal, Alireza Soltanian, Mansour Sajadipoor
There is no reliable estimate of the size of female sex workers (FSWs). This study aimed to estimate the size of FSWs in south of Tehran, Iran in 2016 using direct capture-recapture method. In the capture phase, the hangouts of FSWs were mapped as their meeting places. FSWs who agreed to participate in the study tagged with a T-shirt. The recapture phase was implemented at the same places tagging FSWs with a blue bracelet. The total estimated size of FSWs was 690 (95% CI 633, 747). About 89.43% of FSWs experienced sexual intercourse prior to age 20...
June 16, 2017: AIDS and Behavior
https://www.readbyqxmd.com/read/28618357/visualisation-study-on-pseudomonas-migulae-an-1-transport-in-saturated-porous-media
#5
Dan Qu, Hejun Ren, Rui Zhou, Yongsheng Zhao
Influence of granular size and groundwater flow rate on transport of Pseudomonas migulae AN-1 in saturated porous media was non-invasively and visually investigated with a novel imaging technique based on our previously established green fluorescent protein-tagging approach. AN-1 was transported faster than water was. The finer the media were, the greater the enhancement of bacterial velocity was. Mass recovery (MR) increased, while deposition rate coefficient (Kc) decreased, with increasing granular size. Similar and linear trends of MR and Kc, respectively, were quantitatively observed with increasing water flow rate...
June 7, 2017: Water Research
https://www.readbyqxmd.com/read/28615413/breaking-the-color-barrier-a-multi-selective-antibody-reporter-offers-innovative-strategies-of-fluorescence-detection
#6
Eugenio Gallo, Jonathan W Jarvik
A novel bi-partite fluorescence platform exploits the high affinity and selectivity of antibody scaffolds to capture and activate small molecule fluorogens. In this report we investigated the property of multi-selectivity activation from a single antibody against diverse cyanine-family fluorogens. Our fluorescence screen identified three cell impermeant fluorogens; each with unique emission spectra (blue, green, and red) and nanomolar affinities. Most importantly, as a protein fusion-tag to G-protein-coupled receptors the antibody biosensor retained full activity - displaying bright fluorogen signals with minimal background on live cells...
June 14, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28611392/targeted-sequencing-of-both-dna-strands-barcoded-and-captured-individually-by-rna-probes-to-identify-genome-wide-ultra-rare-mutations
#7
Qing Wang, Xu Wang, Pheobe S Tang, Grace M O'leary, Ming Zhang
Next Generation Sequencing (NGS) has been widely implemented in biological research and has made a profound impact on patient care. One of the essential NGS applications is to identify disease-causing sequence variants, where high coverage and accuracy are needed. Here, we reported a novel NGS pipeline, termed a Sequencing System of Digitalized Barcode Encrypted Single-stranded Library from Extremely Low (quality and quantity) DNA Input with Probe-based DNA Enrichment by RNA probes targeting DNA duplex (DEEPER-Seq)...
June 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28608671/conformational-control-of-udp-galactopyranose-mutase-inhibition
#8
Kittikhun Wangkanont, Valerie J Winton, Katrina T Forest, Laura L Kiessling
UDP-galactopyranose mutase (Glf or UGM) catalyzes the formation of uridine 5'-diphosphate-α-D-galactofuranose (UDP-Galf) from UDP-galactopyranose (UDP-Galp). The enzyme is required for the production of Galf-containing glycans. UGM is absent in mammals, but members of the Corynebacterineae suborder require UGM for cell envelope biosynthesis. The need for UGM in some pathogens has prompted the search for inhibitors that could serve as antibiotic leads. Optimizing inhibitor potency, however, has been challenging...
June 13, 2017: Biochemistry
https://www.readbyqxmd.com/read/28605454/stacks-off-tracks-a-role-for-the-golgin-atcasp-in-plant-endoplasmic-reticulum-golgi-apparatus-tethering
#9
Anne Osterrieder, Imogen A Sparkes, Stan W Botchway, Andy Ward, Tijs Ketelaar, Norbert de Ruijter, Chris Hawes
The plant Golgi apparatus modifies and sorts incoming proteins from the endoplasmic reticulum (ER) and synthesizes cell wall matrix material. Plant cells possess numerous motile Golgi bodies, which are connected to the ER by yet to be identified tethering factors. Previous studies indicated a role for cis-Golgi plant golgins, which are long coiled-coil domain proteins anchored to Golgi membranes, in Golgi biogenesis. Here we show a tethering role for the golgin AtCASP at the ER-Golgi interface. Using live-cell imaging, Golgi body dynamics were compared in Arabidopsis thaliana leaf epidermal cells expressing fluorescently tagged AtCASP, a truncated AtCASP-ΔCC lacking the coiled-coil domains, and the Golgi marker STtmd...
June 9, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28593740/combination-of-mass-signal-amplification-and-isotope-labeled-alkanethiols-for-the-multiplexed-detection-of-mirnas
#10
Hyunook Kang, Seol-Hye Hong, Jiha Sung, Woon-Seok Yeo
We report a fast and sensitive method for the multiplexed detection of miRNAs by combining mass signal amplification and isotope-labeled signal reporter molecules. In our strategy, target miRNAs are captured specifically by immobilized DNAs on gold nanoparticles (AuNPs), which carry a large number of small molecules, called amplification tags (Am-tags), as the reporter for the detection of target miRNAs. For multiplexed detection, we designed and synthesized four Am-tags containing 0, 4, 8, 12 isotopes so that they had same molecular properties but different molecular weights...
June 7, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28592540/covalent-linkage-of-hiv-1-trimers-to-synthetic-liposomes-elicits-improved-b-cell-and-antibody-responses
#11
Shridhar Bale, Geraldine Goebrecht, Armando Stano, Richard Wilson, Takayuki Ota, Karen Tran, Jidnyasa Ingale, Michael B Zwick, Richard T Wyatt
We have demonstrated that a liposomal array of well-ordered trimers enhances B cell activation, germinal center formation and the elicitation of tier-2 autologous neutralizing antibodies. Previously, we coupled well-ordered cleavage-independent NFL trimer via their C-terminal poly-histidine tails to nickel-lipids integrated into the lipid bilayer. Despite favorable in vivo effects, concern remains over the potentially longer term in vivo instability of non-covalent linkage of the trimers to the liposomes. Accordingly, we tested both cobalt coupling and covalent linkage of the trimers to the liposomes by reengineering the poly-histidine tail to include a free cysteine on each protomer of model BG505 NFL trimers to allow covalent linkage...
June 7, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28590745/sensitive-detection-of-staphylococcus-aureus-with-vancomycin-conjugated-magnetic-beads-as-enrichment-carriers-combined-with-flow-cytometry
#12
Xiangyu Meng, Guotai Yang, Fulai Li, Taobo Liang, Weihua Lai, Hengyi Xu
A novel sandwich strategy was designed to detect Staphylococcus aureus. The strategy is based on an antibacterial agent that captures bacterial cells and a fluorescein-labeled antibody that acts as the signal-output probe. Vancomycin (Van), which exerts a strong antibacterial effect on Gram-positive bacteria, was utilized as a molecular recognition agent to detect pathogenic bacteria. To effectively concentrate S. aureus, we used bovine serum albumin (BSA) as the amplification carrier to modify magnetic beads (MBs), which were then functionalized with Van...
June 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28589347/fate-mapping-and-transcript-profiling-of-germinal-center-cells-by-two-photon-photoconversion
#13
Imogen Moran, Tri Giang Phan
The germinal center (GC) reaction is the key process for the generation of high affinity antibodies to foreign antigen. Standard experimental techniques such as fluorescence-activated cell sorting and histology have provided numerous insights into the composition and function of the GC. However, these approaches are limited to a "snapshot" in time and are unable to fully capture the dynamic nature of the GC. Intravital two-photon microscopy overcomes these disadvantages and has led to several major advances in the field but is restricted by practical and technical limits that prevent long-range mapping and molecular studies...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28585016/ecotoxicological-assessment-of-pesticides-and-their-combination-on-rhizospheric-microbial-community-structure-and-function-of-vigna-radiata
#14
Varsha Ashok Walvekar, Swati Bajaj, Dileep K Singh, Shilpi Sharma
India is one of the leading countries in production and indiscriminate consumption of pesticides. Owing to their xenobiotic nature, pesticides affect soil microorganisms that serve as mediators in plant growth promotion. Our study aimed to deliver a comprehensive picture, by comparing the effects of synthetic pesticides (chlorpyriphos, cypermethrin, and a combination of both) with a biopesticide (azadirachtin) at their recommended field application level (L), and three times the recommended dosage (H) on structure and function of microbial community in rhizosphere of Vigna radiata...
June 6, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28581639/traceless-cleavage-of-protein-biotin-conjugates-under-biologically-compatible-conditions
#15
Joseph Cowell, Matthew Buck, Ali H Essa, Rebecca Clarke, Waldemar Vollmer, Daniela Vollmer, Catharien Hilkens, John Isaacs, Michael J Hall, Joe Gray
Biotinylation of amines is widely used to conjugate biomolecules, but the resulting label is either non-removable or removal leaves a tag on the molecule of interest, affecting downstream processes. We present here a set of reagents (RevAmines) that allow traceless, reversible biotinylation under biologically-compatible, mild conditions. Release following avidin-based capture is achieved through the cleavage of a (2-(alkylsulfonyl)ethyl) carbamate linker under mild conditions (200 mM ammonium bicarbonate, pH 8, 16 - 24 h, r...
June 5, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28554047/ultrasensitive-detection-of-aflatoxin-b1-by-sers-aptasensor-based-on-exonuclease-assisted-recycling-amplification
#16
Qin Li, Zhicheng Lu, Xuecai Tan, Xiaoyan Xiao, Pan Wang, Long Wu, Kang Shao, Wenmin Yin, Heyou Han
Aflatoxin B1 (AFB1) is one of the most abundant and carcinogenic food-contaminating mycotoxins around the world. In this study, we proposed a surface enhanced Raman scattering (SERS) sensing strategy for the determination of AFB1. An aptamer for AFB1 partially hybridized with complementary-DNA, which was released after the recognition of AFB1 and immediately hybridized with hairpin DNA on the surface of sputtering Au film. Exonuclease III hydrolyzed the double-stranded DNA, leaving short single-stranded DNA on the Au surface and releasing complementary-DNA for next ring opening and digestion...
May 17, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28542437/cp5-system-for-simple-and-highly-efficient-protein-purification-with-a-c-terminal-designed-mini-tag
#17
Hiroyuki Takeda, Wei Zhou, Kohki Kido, Ryoji Suno, Takahiro Iwasaki, Takuya Kobayashi, Tatsuya Sawasaki
There are many strategies to purify recombinant proteins of interest, and affinity purification utilizing monoclonal antibody that targets a linear epitope sequence is one of the essential techniques used in current biochemistry and structural biology. Here we introduce a new protein purification system using a very short CP5 tag. First, we selected anti-dopamine receptor D1 (DRD1) rabbit monoclonal antibody clone Ra62 (Ra62 antibody) as capture antibody, and identified its minimal epitope sequence as a 5-amino-acid sequence at C-terminal of DRD1 (GQHPT-COOH, D1CE sequence)...
2017: PloS One
https://www.readbyqxmd.com/read/28540944/an-ultrasensitive-multi-walled-carbon-nanotube-platinum-luminol-nanocomposite-based-electrochemiluminescence-immunosensor
#18
Xin Zhang, Hong Ke, Zhiming Wang, Weiwei Guo, Amin Zhang, Chusen Huang, Nengqin Jia
An ultrasensitive electrochemiluminescence (ECL) immunosensor for carbohydrate antigen 19-9 (CA19-9) detection using multi-walled carbon nanotube-platinum-luminol nanocomposites (MWCNT-Pt-luminol) as nanointerface and signal tags was designed. First, the MWCNT-Pt-luminol nanocomposite was decorated on the surface of a glassy carbon electrode (GCE) through the film-forming properties of chitosan (Chi). Then, the CA19-9 antibody (anti-CA19-9) was attached to the modified electrode surface via crosslinking with glutaraldehyde (GA)...
May 25, 2017: Analyst
https://www.readbyqxmd.com/read/28538990/measuring-nonlinear-signal-combination-using-eeg
#19
Darren G M Cunningham, Daniel H Baker, Jonathan W Peirce
Relatively little is known about the processes, both linear and nonlinear, by which signals are combined beyond V1. By presenting two stimulus components simultaneously, flickering at different temporal frequencies (frequency tagging) while measuring steady-state visual evoked potentials, we can assess responses to the individual components, including direct measurements of suppression on each other, and various nonlinear responses to their combination found at intermodulation frequencies. The result is a rather rich dataset of frequencies at which responses can be found...
May 1, 2017: Journal of Vision
https://www.readbyqxmd.com/read/28532419/a-survey-of-the-complex-transcriptome-from-the-highly-polyploid-sugarcane-genome-using-full-length-isoform-sequencing-and-de-novo-assembly-from-short-read-sequencing
#20
Nam V Hoang, Agnelo Furtado, Patrick J Mason, Annelie Marquardt, Lakshmi Kasirajan, Prathima P Thirugnanasambandam, Frederik C Botha, Robert J Henry
BACKGROUND: Despite the economic importance of sugarcane in sugar and bioenergy production, there is not yet a reference genome available. Most of the sugarcane transcriptomic studies have been based on Saccharum officinarum gene indices (SoGI), expressed sequence tags (ESTs) and de novo assembled transcript contigs from short-reads; hence knowledge of the sugarcane transcriptome is limited in relation to transcript length and number of transcript isoforms. RESULTS: The sugarcane transcriptome was sequenced using PacBio isoform sequencing (Iso-Seq) of a pooled RNA sample derived from leaf, internode and root tissues, of different developmental stages, from 22 varieties, to explore the potential for capturing full-length transcript isoforms...
May 22, 2017: BMC Genomics
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