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Tagging and capture

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https://www.readbyqxmd.com/read/28079282/troubling-issues-at-the-frontier-of-animal-tracking-for-conservation-and-management
#1
Steven J Cooke, Vivian M Nguyen, Steven T Kessel, Nigel E Hussey, Nathan Young, Adam T Ford
Developments in electronic tagging and tracking, including biotelemetry and biologging, have provided unprecedented insight into the ecology of wild animals (Cooke et al. 2004) and revealed hidden movement patterns, habitat associations, animal-environment interactions, and mortality rates for even the most cryptic of species (Hussey et al. 2015; Kays et al. 2015). Natural history, ecology (including movement ecology), conservation, and resource management have all benefitted from the application of this technology...
January 12, 2017: Conservation Biology: the Journal of the Society for Conservation Biology
https://www.readbyqxmd.com/read/28069507/a-highly-versatile-adaptor-protein-for-the-tethering-of-growth-factors-to-gelatin-based-biomaterials
#2
Cyril Addi, Frédéric Murschel, Benoît Liberelle, Nesrine Riahi, Gregory De Crescenzo
: In the field of tissue engineering, the tethering of growth factors to tissue scaffolds in an oriented manner can enhance their activity and increase their half-life. We chose to investigate the capture of the basic Fibroblast Growth Factor (bFGF) and the Epidermal Growth Factor (EGF) on a gelatin layer, as a model for the functionalization of collagen-based biomaterials. Our strategy relies on the use of two high affinity interactions, that is, the one between two distinct coil peptides as well as the one occurring between a collagen-binding domain (CBD) and gelatin...
January 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28065806/-tagging-along-memories-in-aging-synaptic-tagging-and-capture-mechanisms-in-the-aged-hippocampus
#3
REVIEW
Mahesh Shivarama Shetty, Sreedharan Sajikumar
Aging is accompanied by a general decline in the physiological functions of the body with the deteriorating organ systems. Brain is no exception to this and deficits in cognitive functions are quite common in advanced aging. Though a variety of age-related alterations are observed in the structure and function throughout the brain, certain regions show selective vulnerability. Medial temporal lobe, especially the hippocampus, is one such preferentially vulnerable region and is a crucial structure involved in the learning and long-term memory functions...
January 5, 2017: Ageing Research Reviews
https://www.readbyqxmd.com/read/28049710/accuracy-of-genomic-prediction-in-synthetic-populations-depending-on-the-number-of-parents-relatedness-and-ancestral-linkage-disequilibrium
#4
Pascal Schopp, Dominik Müller, Frank Technow, Albrecht E Melchinger
Synthetics play an important role in quantitative genetic research and plant breeding, but few studies have investigated the application of genomic prediction (GP) to these populations. Synthetics are generated by intermating a small number of parents ([Formula: see text] and thereby possess unique genetic properties, which make them especially suited for systematic investigations of factors contributing to the accuracy of GP. We generated synthetics in silico from [Formula: see text]2 to 32 maize (Zea mays L...
January 2017: Genetics
https://www.readbyqxmd.com/read/28036085/rfid-ownership-transfer-with-positive-secrecy-capacity-channels
#5
Jorge Munilla, Mike Burmester, Alberto Peinado, Guomin Yang, Willy Susilo
RFID ownership transfer protocols (OTPs) transfer tag ownership rights. Recently, there has been considerable interest in such protocols; however, guaranteeing privacy for symmetric-key settings without trusted third parties (TTPs) is a challenge still unresolved. In this paper, we address this issue and show that it can be solved by using channels with positive secrecy capacity. We implement these channels with noisy tags and provide practical values, thus proving that perfect secrecy is theoretically possible...
December 29, 2016: Sensors
https://www.readbyqxmd.com/read/28003065/easy-detection-of-multiple-alexandrium-species-using-dna-chromatography-chip
#6
Satoshi Nagai, Shigehiko Miyamoto, Keita Ino, Seisuke Tajimi, Hiromi Nishi, Jun Tomono
In this study, the Kaneka DNA chromatography chip (KDCC) for the Alexandrium species was successfully developed for simultaneous detection of five Alexandrium species. This method utilizes a DNA-DNA hybridization technology. In the PCR process, specifically designed tagged-primers are used, i.e. a forward primer consisting of a tag domain, which can conjugate with gold nanocolloids on the chip, and a primer domain, which can anneal/amplify the target sequence. However, the reverse primer consists of a tag domain, which can hybridize to the solid-phased capture probe on the chip, and a primer domain, which can anneal/amplify the target sequence...
January 2016: Harmful Algae
https://www.readbyqxmd.com/read/28002485/a-combinatory-antibody-antigen-microarray-assay-for-high-content-screening-of-single-chain-fragment-variable-clones-from-recombinant-libraries
#7
Nina Persson, Bo Jansson, Nicolai Stuhr-Hansen, András Kovács, Charlotte Welinder, Lena Danielsson, Ola Blixt
We have developed a combinatory antibody-antigen microarray for direct screening of multiple single-chain fragment variable (scFv) clones with no need for pre-purification or enrichment before screening. The straightforward workflow allows for early selection of binders to predefined peptide and glycopeptide targets. A capture antibody is contact printed on microarray slides, side by side with the antigens of interest. A large number of scFv clones, in supernatants, are printed on top of the capture antibody and the antigen in a "spot-on-spot" print...
2016: PloS One
https://www.readbyqxmd.com/read/28001318/carbon-dioxide-capture-adsorbents-chemistry-and-methods
#8
Hasmukh A Patel, Jeehye Byun, Cafer T Yavuz
Excess carbon dioxide (CO2) emissions and the inevitable consequences continuing to stimulate hard debate and awareness in both academic and public spaces, despite the clutter on understanding what really is needed to capture and store the unwanted CO2. Capture is the most costly, nearly 70 % of the price tag on the entire carbon capture and storage (CCS) operation. In this tutorial review, we describe and evaluate CO2 capture science and technology based on adsorbents in the context of chemistry and methods, after briefly introducing current status of CO2 emissions...
December 21, 2016: ChemSusChem
https://www.readbyqxmd.com/read/27992465/discovering-genome-wide-tag-snps-based-on-the-mutual-information-of-the-variants
#9
Abdulkadir Elmas, Tai-Hsien Ou Yang, Xiaodong Wang, Dimitris Anastassiou
Exploring linkage disequilibrium (LD) patterns among the single nucleotide polymorphism (SNP) sites can improve the accuracy and cost-effectiveness of genomic association studies, whereby representative (tag) SNPs are identified to sufficiently represent the genomic diversity in populations. There has been considerable amount of effort in developing efficient algorithms to select tag SNPs from the growing large-scale data sets. Methods using the classical pairwise-LD and multi-locus LD measures have been proposed that aim to reduce the computational complexity and to increase the accuracy, respectively...
2016: PloS One
https://www.readbyqxmd.com/read/27976690/capillary-isoelectric-focusing-after-sample-enrichment-with-immunoaffinity-chromatography-in-a-single-capillary
#10
Kiyohito Shimura, Toshihiko Nagai
For accurate micro-scale quantification of a specific protein in biological fluids, immunoaffinity chromatography (IAC) and isoelectric focusing (IEF) were combined in a single fused-silica capillary. The inner wall of the capillary was coated with an anti-E-tag antibody at the inlet side to form an IAC column, and polydimethylacrylamide, a neutral polymer, at the outlet side to form the capillary for IEF. After loading a sample, the whole capillary was filled with a carrier ampholyte solution. An anode solution, an acid, was then introduced to fill only the IAC column segment...
December 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27974384/brca-testing-by-single-molecule-molecular-inversion-probes
#11
Kornelia Neveling, Arjen R Mensenkamp, Ronny Derks, Michael Kwint, Hicham Ouchene, Marloes Steehouwer, Bart van Lier, Ermanno Bosgoed, Alwin Rikken, Marloes Tychon, Dimitra Zafeiropoulou, Steven Castelein, Jayne Hehir-Kwa, Djie Tjwan Thung, Tom Hofste, Stefan H Lelieveld, Stijn M M Bertens, Ivo B J F Adan, Astrid Eijkelenboom, Bastiaan B Tops, Helger Yntema, Tomasz Stokowy, Per M Knappskog, Hildegunn Høberg-Vetti, Vidar M Steen, Evan Boyle, Beth Martin, Marjolijn J L Ligtenberg, Jay Shendure, Marcel R Nelen, Alexander Hoischen
BACKGROUND: Despite advances in next generation DNA sequencing (NGS), NGS-based single gene tests for diagnostic purposes require improvements in terms of completeness, quality, speed, and cost. Single-molecule molecular inversion probes (smMIPs) are a technology with unrealized potential in the area of clinical genetic testing. In this proof-of-concept study, we selected 2 frequently requested single gene tests, those for breast cancer 1, early onset (BRCA1) and breast cancer 2, early onset (BRCA2), and developed an automated work flow based on smMIPs...
December 14, 2016: Clinical Chemistry
https://www.readbyqxmd.com/read/27940237/simultaneous-detection-of-mcf-7-and-hepg2-cells-in-blood-by-icp-ms-with-gold-nanoparticles-and-quantum-dots-as-elemental-tags
#12
Xiaoting Li, Beibei Chen, Man He, Han Wang, Guangyang Xiao, Bin Yang, Bin Hu
In this work, we demonstrate a novel method based on inductively coupled plasma mass spectrometry (ICP-MS) detection with gold nanoparticles (Au NPs) and quantum dots (QDs) labeling for the simultaneous counting of two circulating tumor cell lines (MCF-7 and HepG2 cells) in human blood. MCF-7 and HepG2 cells were captured by magnetic beads coupled with anti-EpCAM and then specifically labeled by CdSe QDs-anti-ASGPR and Au NPs-anti-MUC1, respectively, which were used as signal probes for ICP-MS measurement. Under the optimal experimental conditions, the limits of detection of 50 MCF-7, 89 HepG2 cells and the linear ranges of 200-40000 MCF-7, 300-30000 HepG2 cells were obtained, and the relative standard deviations for seven replicate detections of 800 MCF-7 and HepG2 cells were 4...
April 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27930670/ku-binding-on-telomeres-occurs-at-sites-distal-from-the-physical-chromosome-ends
#13
Mélanie V Larcher, Emeline Pasquier, R Stephen MacDonald, Raymund J Wellinger
The Ku complex binds non-specifically to DNA breaks and ensures repair via NHEJ. However, Ku is also known to bind directly to telomeric DNA ends and its presence there is associated with telomere capping, but avoiding NHEJ. How the complex discriminates between a DNA break and a telomeric extremity remains unknown. Our results using a tagged Ku complex, or a chromosome end capturing method, in budding yeast show that yKu association with telomeres can occur at sites distant from the physical end, on sub-telomeric elements, as well as on interstitial telomeric repeats...
December 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27929459/perturbations-of-circulating-mirnas-in-irritable-bowel-syndrome-detected-using-a-multiplexed-high-throughput-gene-expression-platform
#14
Nicolaas H Fourie, Ralph M Peace, Sarah K Abey, LeeAnne B Sherwin, John W Wiley, Wendy A Henderson
The gene expression platform assay allows for robust and highly reproducible quantification of the expression of up to 800 transcripts (mRNA or miRNAs) in a single reaction. The miRNA assay counts transcripts by directly imaging and digitally counting miRNA molecules that are labeled with color-coded fluorescent barcoded probe sets (a reporter probe and a capture probe). Barcodes are hybridized directly to mature miRNAs that have been elongated by ligating a unique oligonucleotide tag (miRtag) to the 3' end...
November 30, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27912002/a-novel-application-of-multi-event-modeling-to-estimate-class-segregation-in-a-highly-migratory-oceanic-vertebrate
#15
T K Chapple, T Chambert, P E Kanive, S J Jorgensen, J J Rotella, S D Anderson, A B Carlisle, B A Block
Spatial segregation of animals by class (i.e., maturity or sex) within a population due to differential rates of temporary emigration (TE) from study sites can be an important life history feature to consider in population assessment and management. However, such rates are poorly known; new quantitative approaches to address these knowledge gaps are needed. We present a novel application of multi-event models that takes advantage of two sources of detections to differentiate temporary emigration from apparent absence to quantify class segregation within a study population of double-marked (photo-identified and tagged with coded acoustic transmitters) white sharks (Carcharodon carcharias) in central California...
December 2016: Ecology
https://www.readbyqxmd.com/read/27908071/measurements-of-humpback-whale-song-sound-levels-received-by-a-calf-in-association-with-a-singer
#16
Jessica Chen, Adam A Pack, Whitlow W L Au, Alison K Stimpert
Male humpback whales produce loud "songs" on the wintering grounds and some sing while escorting mother-calf pairs, exposing them to near-continuous sounds at close proximity. An Acousonde acoustic and movement recording tag deployed on a calf off Maui, Hawaii captured sounds produced by a singing male escort. Root-mean-square received levels ranged from 126 to 158 dB re 1 μPa. These levels represent rare direct measurements of sound to which a newly born humpback calf may be naturally exposed by a conspecific, and may provide a basis for informed decisions regarding anthropogenic sound levels projected near calves...
November 2016: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/27883075/direct-electrical-control-of-igg-conformation-and-functional-activity-at-surfaces
#17
Paola Ghisellini, Marialuisa Caiazzo, Andrea Alessandrini, Roberto Eggenhöffner, Massimo Vassalli, Paolo Facci
We have devised a supramolecular edifice involving His-tagged protein A and antibodies to yield surface immobilized, uniformly oriented, IgG-type, antibody layers with Fab fragments exposed off an electrode surface. We demonstrate here that we can affect the conformation of IgGs, likely pushing/pulling electrostatically Fab fragments towards/from the electrode surface. A potential difference between electrode and solution acts on IgGs' charged aminoacids modulating the accessibility of the specific recognition regions of Fab fragments by antigens in solution...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27881095/a-regional-consensus-recommendation-on-brain-atrophy-as-an-outcome-measure-in-multiple-sclerosis
#18
Raed Alroughani, Dirk Deleu, Khalid El Salem, Jasem Al-Hashel, K John Alexander, Mohamed Assem Abdelrazek, Adel Aljishi, Jaber Alkhaboori, Faisal Al Azri, Nahida Al Zadjali, Majed Hbahbih, Tag Eldin Sokrab, Mohamed Said, Àlex Rovira
BACKGROUND: Multiple sclerosis (MS) is a chronic autoimmune disease characterized by inflammatory and neurodegenerative processes leading to irreversible neurological impairment. Brain atrophy occurs early in the course of the disease at a rate greater than the general population. Brain volume loss (BVL) is associated with disability progression and cognitive impairment in patients with MS; hence its value as a potential target in monitoring and treating MS is discussed. METHODS: A group of MS neurologists and neuro-radiologists reviewed the current literature on brain atrophy and discussed the challenges in assessing and implementing brain atrophy measurements in clinical practice...
November 24, 2016: BMC Neurology
https://www.readbyqxmd.com/read/27880036/protein-phosphotyrosine-proteome-profiling-by-superbinder-sh2-domain-affinity-purification-mass-spectrometry-ssh2-ap-ms
#19
Jiefei Tong, Biyin Cao, Gregory D Martyn, Jonathan R Krieger, Paul Taylor, Bradley Yates, Sachdev S Sidhu, Shawn S C Li, Xinliang Mao, Michael F Moran
Recently, "superbinder" SH2 domain variants with three amino acid substitutions (sSH2) were reported to have 100-fold or greater affinity for protein-phosphotyrosine (pY) than natural SH2 domains. Here we report a protocol in which His-tagged Src sSH2 efficiently captures pY-peptides from protease-digested HeLa cell total protein extracts. Affinity purification of pY-peptides by this method shows little bias for pY-proximal amino acid sequences, comparable to that achieved by using antibodies to pY, but with equal or higher yield...
November 23, 2016: Proteomics
https://www.readbyqxmd.com/read/27867058/application-of-strep-tactin-xt-for-affinity-purification-of-twin-strep-tagged-cb2-a-g-protein-coupled-cannabinoid-receptor
#20
Alexei Yeliseev, Lioudmila Zoubak, Thomas G M Schmidt
Human cannabinoid receptor CB2 belongs to the class A of G protein-coupled receptor (GPCR). High resolution structural studies of CB2 require milligram quantities of purified, structurally intact protein. Here we describe an efficient protocol for purification of this protein using the Twin-Strep-tag/Strep-Tactin XT system. To improve the affinity of interaction of the recombinant CB2 with the resin, the double repeat of the Strep-tag was attached either to the N- or C-terminus of CB2 via a short linker. The CB2 was isolated at high purity from dilute solutions containing high concentrations of detergents, glycerol and salts, by capturing onto the Strep-Tactin XT resin, and was eluted from the resin under mild conditions upon addition of biotin...
November 17, 2016: Protein Expression and Purification
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