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Shohreh Movahedi, Leili Safdarian, Marzieh Agahoseini, Ashraf Aleyasin, Sepideh Khodaverdi, Sara Asadollah, Ali Kord Valeshabad, Parvin Fallahi, Zahra Rezaeeian
BACKGROUND: Release of vascular endothelial growth factor (VEGF) by ovaries in response to HCG administration is one of the main mechanisms of ovarian hyper stimulation syndrome. Since Dopamine/dopamine receptor2 (Dp-r2) pathway activity -mediated by VEGF/ Vascular endothelial growth factor receptor 2 (VEGFR- 2) signaling-, is associated with angiogenic events, dopamine agonists were used for the management of severe forms of OHSS. In order to assess the effects of Cabergoline on angiogenesis in the human endometrium, and subsequently its impacts on the implantation rate this study was conducted...
2016: Medical Journal of the Islamic Republic of Iran
Ensieh Shahrokh Tehraninejad, Elham Azimi Nekoo, Firouzeh Ghaffari, Maryam Hafezi, Leila Karimian, Arezoo Arabipoor
AIM: This study aimed to compare the outcomes between zygote intrafallopian transfer (ZIFT) with intrauterine day-3 (cleavage stage) embryo transfer and intrauterine day-5 (blastocyst stage) embryo transfer in patients undergoing intracytoplasmic sperm injection. MATERIAL AND METHODS: This prospective study was performed at Royan Institute, Tehran, Iran, between January 2012 and January 2014. Two hundred fifty women with more than three unexplained implantation failures were divided non-randomly into three groups according to embryonic age and methods used as follows: (i) intrauterine cleavage-stage embryo transfer (n = 100); (ii) intrauterine blastocyst-stage embryo transfer (n = 50); and (iii) ZIFT (n = 100)...
November 2015: Journal of Obstetrics and Gynaecology Research
Koji Nakagawa, Hiroyasu Juen, Yayoi Nishi, Rie Sugiyama, Hiroshi Motoyama, Yasushi Kuribayashi, Masato Inoue, Shigeo Akira, Rikikazu Sugiyama
PURPOSE: Zygote intrafallopian transfer (ZIFT) is an effective option for patients who have experienced repeated implantation failures (RIF) in assisted reproductive technology (ART) treatment. However, advance planning for the day of the operation can be problematic. Using a hormone replacement cycle (HRC) makes it possible to plan for the day of ZIFT. In the present study, we evaluated whether HRC-ZIFT is useful for RIF patients who have experienced difficulties obtaining morphologically good embryos in vitro...
November 2014: Archives of Gynecology and Obstetrics
Elsa J Whitmore, Jacek Graczykowski, Chiravudh Sawetawan
INTRODUCTION: The objective of this study was to understand how cervical agenesis affects fertility management. Congenital absence of the cervix with functioning endometrial tissue is extremely rare. Primary amenorrhea and cyclic abdominal pain are the usual presentation. Creation of a functional cervix is difficult as a result of a lack of cervical mucus production. Thus, women who desire pregnancy must undergo in vitro fertilization with zygote intrafallopian transfer. METHODS: A 33-year-old nulligravid diagnosed with cervical agenesis, uterine didelphys, and blind vaginal pouch desires fertility...
May 2014: Obstetrics and Gynecology
Itai Gat, Jacob Levron, Gil Yerushalmi, Jehoshua Dor, Masha Brengauz, Raoul Orvieto
BACKGROUND: One of the suggest strategy for patients with repeated implantation failure (RIF) is zygote intrafallopian transfer (ZIFT). However, no data exist regarding to the issue of when and under which circumstances should ZIFT be offered to patients with RIF? We therefore aimed to examine whether repeated implantation failure (RIF) patients characteristics or their previous controlled ovarian hyperstimulation (COH) variables may differentiate between those who will conceive following a ZIFT cycle and those who will not...
2014: Journal of Ovarian Research
Ariel Weissman, Eran Horowitz, Amir Ravhon, Hana Nahum, Abraham Golan, David Levran
OBJECTIVE: To summarize the experience of a single center with laparoscopic zygote intrafallopian transfer (ZIFT) performed exclusively among patients with high-order repeated implantation failure (RIF) following in vitro fertilization-embryo transfer (IVF-ET). METHODS: A retrospective cohort study was performed at the Edith Wolfson Medical Center, a tertiary referral university hospital located in Holon, Israel. A group of 176 patients with 8.15±3.9 previously failed IVF-ET cycles underwent 280 ZIFT procedures between 1995 and 2010...
January 2013: International Journal of Gynaecology and Obstetrics
Emad M Siam
The objective of the study was to investigate whether transferring zygotes on day 1 would result in similar pregnancy rates compared to transferring cleavage stage embryos on day 3 in a prospective randomized trial, using the office microlaparoscopic procedure. Patients undergoing IVF/ICSI treatments were randomized to either day 1 or day 3 transfers after previous failed ICSI trials due to failed implantation. The primary outcome measure was pregnancy rate. Pregnancy rates were higher in day 3 group (55/131, 42%) when compared to day 1 (34/123, 28%, P = 0...
June 2011: African Journal of Reproductive Health
Gernot Tews, Omar Shebl, Marianne Moser, Thomas Ebner
OBJECTIVE: To analyze whether the use of blastocyst intrafallopian transfer is a feasible option in a case of repeated difficult ET. DESIGN: Case report. SETTING: Public hospital. PATIENT(S): Forty-year old nulliparous patient. INTERVENTION(S): Transfer of two vitrified/warmed blastocysts into the right tube by means of laparoscopy. MAIN OUTCOME MEASURE(S): Successful ET, clinical pregnancy...
July 2012: Fertility and Sterility
Teraporn Vutyavanich, Waraporn Piromlertamorn, Jason Ellis, et al.
AIM: To present the results of assisted reproductive technology (ART) performed in Thailand during 2001-2007. METHODS: All licensed ART centers are obliged to submit annual reports on the number of patients, cycles, ART techniques and treatment outcomes to the Reproductive Medicine Subcommittee of the Royal Thai College of Obstetricians and Gynaecologists. Data from all centers were aggregated and analyzed retrospectively. RESULTS: Cycles were categorized into fresh and frozen/thawed embryo transfer (FET) cycles...
March 2011: Journal of Obstetrics and Gynaecology Research
Donna Dowling-Lacey, Jacob F Mayer, Estella Jones, Silvina Bocca, Laurel Stadtmauer, Sergio Oehninger
OBJECTIVE: To report a live birth after transfer of cryopreserved pronuclear embryos in cryostorage for almost 20 years. DESIGN: Case report. SETTING: Academic IVF center. PATIENT(S): A 42-year-old female patient with low ovarian reserve receiving donated embryos. These embryos were the result of an anonymous donation from an infertile couple who had conceived during her IVF treatment. INTERVENTION(S): Cryopreservation (slow-freeze method) and thawing of pronuclear stage embryos, and ultrasound-guided uterine ET...
March 1, 2011: Fertility and Sterility
Eric Surrey, Jennifer Keller, John Stevens, Robert Gustofson, Debra Minjarez, William Schoolcraft
This retrospective cohort study compared outcomes from transfer of embryos cryopreserved at the pronuclear versus blastocyst stage following 'freeze-all' IVF cycles without fresh transfer for 87 consecutive IVF patients <40 years, who underwent cryopreservation of all viable embryos followed by at least one subsequent frozen embryo transfer (FET) between January 2003 and July 2007. Cryopreservation of all embryos from one oocyte retrieval was performed at either the pronuclear (1.5 mol/l propanediol and 0...
September 2010: Reproductive Biomedicine Online
Alessia Nicoli, Francesco Capodanno, Lucia Moscato, Ilaria Rondini, Maria T Villani, Antonella Tuzio, Giovanni B La Sala
BACKGROUND: Embryos selection is crucial to maintain high performance in terms of pregnancy rate, reducing the risk of multiple pregnancy during IVF. Pronuclear and nucleolar characteristics have been proposed as an indicator of embryo development and chromosomal complement in humans, providing information about embryo viability. METHODS: To correlate the zygote-score with the maternal age and the outcome of pregnancy, we analyzed the pronuclear and nucleolar morphology, the polar body alignment and the zygote configuration in 459 clinical pregnancies obtained by IVF and ICSI in our public clinic in Reggio Emilia, Italy...
2010: Reproductive Biology and Endocrinology: RB&E
Evgenia Isachenko, Vladimir Isachenko, Plamen Todorov, Vasiliy Ostashko, Rolf Kreienberg, Manfred Kaufmann, Karl Sterzik, Inka Wiegratz
OBJECTIVE: To report a successful pregnancy after transfer of embryos derived from oocytes after calcium ionophore correction of the pronuclei localization after intracytoplasmic sperm injection (ICSI). DESIGN: Case report. SETTING: University hospital. PATIENT(S): A 30-year-old patient and her 30-year-old husband, diagnosed with asthenoteratozoopermia, underwent ICSI because of three unsuccessful IUI attempts. INTERVENTION(S): Thirteen metaphase II oocytes were injected with morphologically normal spermatozoa and immediately divided into two groups...
December 2010: Fertility and Sterility
Hye Jin Chang, Chang Suk Suh
PURPOSE OF REVIEW: This review discusses recent publications that investigate risk factors associated with ectopic pregnancy after IVF. RECENT FINDINGS: Data on the risk factors for developing ectopic pregnancy after IVF are still inconsistent. Between fresh nondonor IVF and embryo transfer cycles, the significant risk factor for ectopic pregnancy was tubal factor infertility, and endometriosis, rather than male factor infertility. Higher ectopic pregnancy rate could be associated with zygote intrafallopian transfer, assisted hatching, large embryo transfer volume, deep fundal transfer, and frozen embryo transfer...
June 2010: Current Opinion in Obstetrics & Gynecology
M Agha-Hosseini, A Aleyaseen, S Peyvandi, L Kasha
We carried out a prospective randomized trial on 220 couples with nontubal factor infertility to compare pregnancy rates and implantation rates after zygote intrafallopian transfer (ZIFT) and uterine embryo transfer (UET). The zygote was transferred by laparoscopy into the fallopian tube 24 hours after oocyst retrieval. UET was performed 72 hours after retrieval with abdominal sonography guide. Transfer was performed in 102 cycles in the ZIFT and 100 cycles in the U ET group. The pregnancy and implantation rates were significantly higher in the ZIFT group (42...
January 2010: Eastern Mediterranean Health Journal, la Revue de Santé de la Méditerranée Orientale
Mai Fujii, Ryu Matsuoka, Eduardo Bergel, Sheryl van der Poel, Takashi Okai
OBJECTIVE: To assess perinatal risks to singleton births after in vitro fertilization (IVF) versus spontaneous conception. DESIGN: Cross-sectional. SETTING: A 2006 registry database of the Japan Society of Obstetrics and Gynaecology (JSOG) capturing 5.8% of total births. PATIENT(S): 53,939 singleton births from spontaneous conceptions and 1,408 singletons after IVF. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Perinatal death, low-birth weight (LBW), small for gestational age (SGA), congenital malformation, and sex ratio assessment based on singleton birth cases versus singleton live-born cases...
November 2010: Fertility and Sterility
J H Check, D Summers-Chase, D Horwath, K Swenson, W Yuan
PURPOSE: To compare the efficacy of freezing embryos at the 2 pronuclear stage vs multi-cell stage using a simplified freezing protocol with a one-step removal of the cryoprotectant. METHODS: A retrospective analysis was performed. Survival, delivered pregnancy and implantation rates were compared in transfers of all embryos frozen at 2 pronuclear stage (2PN) or all embryos frozen at multi-cell stage. The results were further stratified and compared according to the number of high quality embryos transferred...
2009: Clinical and Experimental Obstetrics & Gynecology
H Bagis, T Akkoc, C Taskin, S Arat
The objective of this study was to investigate the effects of beta-mercaptoethanol (β-ME) on post-thaw embryo developmental competence and implantation rate of mouse pronuclear (PN) embryos that were cryopreserved after slow freezing, solid surface vitrification (SSV) or open-pulled straw (OPS) vitrification methods. Mouse PN embryos were cryopreserved by using slow freezing, SSV and OPS methods. After cryopreservation, freeze-thawed PN embryos were cultured up to blastocyst stage in a defined medium supplemented without or with 50 μM β-ME...
December 2010: Reproduction in Domestic Animals, Zuchthygiene
Qing Xu, Stewart A Anderson
As the brain develops, progenitor cells acquire the features of specific neuronal or glial subtypes through dynamic expression of the fate-determining signaling molecules and their targeting transcription factors. An effective and versatile approach for tracing lineage of progenitors into adult cell types is to target the promoter of an interested gene with Cre (a phage DNA recombinase) to achieve simultaneous activation during neurogenesis. The bacterial artificial chromosome (BAC) is an efficient Cre carrier...
January 2010: Current Protocols in Neuroscience
J Ohl
A cervical stenosis can follow an infection, a conisation or a trachelectomy and lead to a cervical infertility. In in vitro fertilization (IVF), embryo transfers are difficult in case of a cervical stenosis and specific care is required. Treatments try to restore an adequate cervical patency. Repeated cervical dilatations can represent a solution. In case of inefficiency, a new cervicoisthmic opening has to be created with surgery. Perpetuity of the result is obtained thanks to post surgical dilatations or thanks to implementation of an intracervical catheter till complete cicatrization...
November 2009: Gynécologie, Obstétrique & Fertilité
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