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Estrogen and oxygen induced retinopathy

Ji Yeon Do, Young Keun Choi, Hyun Kook, Kyoungho Suk, In-Kyu Lee, Dong Ho Park
Ischemic retinopathies causing overexpression of pro-angiogenic factors, including vascular endothelial growth factor (VEGF), are the most common cause of blindness. Thus, understanding the pathophysiology of targetable pathways that regulate retinal VEGF is of great interest. A conserved binding site for estrogen-related receptor γ (ERRγ) has been identified in the promoter of the Vegfa gene. ERRγ is a constitutively active orphan nuclear receptor and its expression is increased by hypoxic stimuli in metabolically active tissues...
May 1, 2015: Biochemical and Biophysical Research Communications
Hongbing Zhang, Xiaodong Wang, Kun Xu, Yao Wang, Yani Wang, Xianning Liu, Xianjiao Zhang, Liang Wang, Xiaogang Li
Retinopathy of prematurity (ROP) is a major and leading cause of blindness in premature infants. It has been realized that early treatment for ROP is important. However, all the early treatments of ROP are focusing on peripheral retinal ablation which does not surmount the limit of extinguishing retinal neovascularization and protecting the retinas of children with ROP from the injury of ablation. In this study, we investigated the morphological changes of retina and oxidative stress alterations in the early phase of oxygen-induced retinopathy (OIR) and tested the effects of 17β-estradiol (17β-E2), a nonselective estrogen receptor (ER) agonist, on early phase OIR development...
February 20, 2015: Biochemical and Biophysical Research Communications
Ming Hao, Yue Li, Wenjian Lin, Qian Xu, Ning Shao, Yixin Zhang, Hongyu Kuang
BACKGROUND: Diabetic retinopathy (DR) is a leading cause of acquired blindness in adults. Previous research has shown that the apoptosis of retinal ganglion cells(RGCs) plays an important role in the initiation and development of diabetic retinopathy. The positive effect of estrogen on the nervous system is currently attracting increasing attention. In this study, we investigated whether17β-estradiolum (E2) has protective effects on RGCs in a high-glucose environment. METHODS: The cell survival rates were measured by Cell Counting Kit-8, the apoptosis was detected by flow cytometry, the intracellular reactive oxygen species (ROS) levels were examined by immunofluorescence method, and the intracellular mitochondrial membrane potential was examined by confocal microscopy...
January 2015: Graefe's Archive for Clinical and Experimental Ophthalmology
Anand Giddabasappa, Jeetendra R Eswaraka, Christina M Barrett, Matthew N Bauler, Zhongzhi Wu, Muralimohan Yepuru, Duane D Miller, James T Dalton
PURPOSE: The goal of our study was to evaluate the in vitro and in vivo anti-angiogenic effects of ERβ selective agonist, β-LGND2, using human retinal microvascular endothelial cell (HRMVEC) cultures and a mouse model for oxygen-induced retinopathy (OIR). METHODS: The selectivity of β-LGND2 was determined using binding and transactivation assays. The effects of β-LGND2 on pathologic neovascularization were evaluated in OIR mice by histology and retinal mounts stained with isolectin B4 to quantify aberrant angiogenesis...
2012: Investigative Ophthalmology & Visual Science
Hong-Bing Zhang, Nai-Xue Sun, Hou-Cheng Liang, Xiang-Hua Xiao, Xian-Ning Liu, Ya-Ni Wang
PURPOSE: Our aim was to explore the effects and mechanism of 17-alpha-estradiol (17α-E2) on oxygen-induced retinopathy (OIR) in a murine model. METHODS: Newborn mice exposed to hyperoxia underwent subcutaneous injections of different doses of 17α-E2 from postnatal days (PND) 7 to 17. The retinal flat mounts were scored for avascular/total retinal area on PND 17. Vascular endothelial growth factor (VEGF), malondialdehyde (MDA) concentrations, and intensity, activity, and quality of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase in the retina were determined on PND 9, 13 (14), and 17...
July 2012: Japanese Journal of Ophthalmology
Xiaohua Ma, Hongsheng Bi, Yi Qu, Xiaofeng Xie, Jinghai Li
OBJECTIVE: To investigate the influence of Estradial (E(2)) at physiological concentration on mRNA expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1α(HIF-α1) in bovine retinal vascular endothelial cells (BRECs) under different oxygen conditions. METHODS: The mRNA expression of VEGF, HIF-1α in BRECs was studied by quantitative reverse-transcription PCR (qRT-PCR), and protein levels were assayed by Western Blot. Different concentrations of E(2) (10(-12), 10(-10), 10(-8) mol/l) and estrogen receptor antagonist Tamoxifen (10(-7) mol/l) were added into the cell culture medium of different groups, while the phosphate-buffered saline (PBS) was added in the control group instead...
June 2011: Graefe's Archive for Clinical and Experimental Ophthalmology
Stephanie Huppmann, Susanne Römer, Rodica Altmann, Michael Obladen, Monika Berns
In premature infants, oxygen free radicals generated following neonatal resuscitation are associated with subsequent diseases such as retinopathy of prematurity and bronchopulmonary dysplasia. Recent studies in brain tissue samples have shown that nonphysiologic oxygen levels play a key role in induction of apoptosis in the developing brain. Estrogen is a well-established agent in neuroprotection and, therefore, is thought to be neuroprotective even in the premature brain. Astrocytes appear to have a critical role in protection and survival of neurons in the brain...
November 15, 2008: Journal of Neuroscience Research
Noriko Miyamoto, Michiko Mandai, Hitoshi Takagi, Izumi Suzuma, Kiyoshi Suzuma, Shinji Koyama, Atsushi Otani, Hideyasu Oh, Yoshihito Honda
PURPOSE: It has been reported that 17beta-estradiol (E2) may enhance the proliferation of bovine retinal vascular endothelial cells (BRECs) by increasing the expression of VEGFR-2 and VEGF. The hypothesis in the current study was that estrogen may contribute to fetal vascular development and the cessation of exposure to estrogen of premature infants on birth may have an inhibitory effect on retinopathy of prematurity (ROP). Because ROP is thought to develop under relative hypoxia after exposure to high-dose oxygen, this study was conducted to investigate how estrogen modulates hypoxia-induced VEGF in BRECs and mouse ROP...
June 2002: Investigative Ophthalmology & Visual Science
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