keyword
MENU ▼
Read by QxMD icon Read
search

PKA

keyword
https://www.readbyqxmd.com/read/29783019/total-flavonoid-extract-of-epimedium-herb-increases-the-peak-bone-mass-of-young-rats-involving-enhanced-activation-of-the-ac10-camp-pka-creb-pathway
#1
Hui-Rong Xi, Hui-Ping Ma, Fang-Fang Yang, Yu-Hai Gao, Jian Zhou, Yuan-Yuan Wang, Wen-Yuan Li, Cory J Xian, Ke-Ming Chen
ETHNOPHARMACOLOGICAL RELEVANCE: Epimedium sagittatum brevicornum Maxim. is an important traditional Chinese herb that has long been used to promote bone fracture healing and treat osteoporosis. AIM OF THE STUDY: Achieving peak bone mass by adolescence has now been accepted to be fundamental for preventing osteoporosis in adulthood life. This study investigated the possibility of increasing peak bone mass in young rats using the total flavonoid extract of Epimedium herb (TFE)...
May 18, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29781009/electrically-controlled-drug-release-using-ph-sensitive-polymer-films
#2
S Ephraim Neumann, Christian F Chamberlayne, Richard N Zare
Drug delivery systems (DDS) that allow spatially and temporally controlled release of drugs are of particular interest in the field of drug delivery. These systems create opportunities for individually tailored doses of drugs to be administered as well as reduce side effects by localizing the initial drug dose to the organ of interest. We present an electroresponsive DDS in the form of a bioresorbable nanocomposite film which operates at low voltages (<-2 V). The method is based on electrochemically generating local pH changes at an electrode surface to induce dissolution of a pH-sensitive polymer, which is used as the carrier material...
May 21, 2018: Nanoscale
https://www.readbyqxmd.com/read/29778625/the-mediating-role-of-phosphodiesterase-type-4-in-the-dopaminergic-modulation-of-motor-impulsivity
#3
P R A Heckman, A Blokland, N P Van Goethem, B T J Van Hagen, J Prickaerts
The current study investigated the mediating role of phosphodiesterase type 4 (PDE4) regulated cAMP in the dopaminergic modulation of premature responding (action restraint) in rats. Response inhibition, which includes action restraint, finds its neurobiological origin in cortico-striatal-thalamic circuitry and can be modulated by dopamine. Intracellularly, the effect of dopamine is largely mediated through the cAMP/PKA signaling cascade. Areas in the prefrontal cortex are very sensitive to their neurochemical environment, including catecholamine levels...
May 17, 2018: Behavioural Brain Research
https://www.readbyqxmd.com/read/29777929/substituent-effect-on-the-acid-induced-isomerization-of-spiropyran-compounds
#4
Lixia Cui, Honghong Zhang, Guomei Zhang, Ying Zhou, Li Fan, Lihong Shi, Caihong Zhang, Shaomin Shuang, Chuan Dong
Spiropyran compounds are well known as an isomeric system, the closed spiropyran (SP) could be converted into the open merocyanine (MC) via acid-induced because stable protonated merocyanine (MCH) were formed by combination of MC and H+ . In order to understand how the substituent affect the isomerization of spiropyran. A series of the chromene 7-subsituted spiropyran compounds were designed and synthesized. The photophysical properties of them were studied comparatively by UV-absorption and fluorescence spectra in different pH...
May 16, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29776674/migration-behavior-of-organic-dyes-based-on-physicochemical-properties-of-solvents-as-background-electrolytes-in-non-aqueous-capillary-electrophoresis
#5
Minjeong Gu, Keunchang Cho, Seong Ho Kang
The migration behavior of organic fluorescent dyes (i.e., crystal violet, methyl violet base, methyl violet B base, rhodamine 6G, and rhodamine B base) in non-aqueous capillary electrophoresis (NACE) was investigated by focusing on the physicochemical properties of various organic solvents [ethanol, methanol, 2-propanol, dimethylformamide (DMF), and dimethyl sulfoxide (DMSO)] in background electrolyte (BGE). Laser-induced fluorescence (LIF) and UV/Vis detectors were employed to observe both the migration time of organic dyes and the electroosmotic flow (EOF) in NACE, respectively...
May 15, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29775474/the-cryptococcus-neoformans-titan-cell-is-an-inducible-and-regulated-morphotype-underlying-pathogenesis
#6
Ivy M Dambuza, Thomas Drake, Ambre Chapuis, Xin Zhou, Joao Correia, Leanne Taylor-Smith, Nathalie LeGrave, Tim Rasmussen, Matthew C Fisher, Tihana Bicanic, Thomas S Harrison, Marcel Jaspars, Robin C May, Gordon D Brown, Raif Yuecel, Donna M MacCallum, Elizabeth R Ballou
Fungal cells change shape in response to environmental stimuli, and these morphogenic transitions drive pathogenesis and niche adaptation. For example, dimorphic fungi switch between yeast and hyphae in response to changing temperature. The basidiomycete Cryptococcus neoformans undergoes an unusual morphogenetic transition in the host lung from haploid yeast to large, highly polyploid cells termed Titan cells. Titan cells influence fungal interaction with host cells, including through increased drug resistance, altered cell size, and altered Pathogen Associated Molecular Pattern exposure...
May 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29775280/fluorine-meets-amine-reducing-microenvironment-induced-amino-activatable-nanoprobes-for-19f-magnetic-resonance-imaging-of-bio-thiols
#7
Pingsheng Huang, Weisheng Guo, Guang Yang, Huijuan Song, Yuqing Wang, Chun Wang, Deling Kong, Weiwei Wang
19F MRI is of great significance for non-invasive imaging and detection of various diseases. However, the main obstacle in the application of 19F MRI agents stems from the unmet signal sensitivity, due to the poor water-solubility and restricted mobility of segments with high numbers of fluorine atoms. Herein, we report a kind of intracellular reducing microenvironment-induced amino-activatable 19F MRI nanoprobe, which can be used for specific imaging of bio-thiols. In principle, the nanoprobe has an initial architecture of hydrophobic core, where the trifluoromethyl-containing segments are compactly packed and 19F NMR/MRI signals are quenched ("OFF" state)...
May 18, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29773583/protein-kinase-a-activation-something-new-under-the-sun
#8
F Donelson Smith, John D Scott
The role of autophosphorylation of the type II regulatory subunit in activation of protein kinase A (PKA) has been a longstanding question. In this issue, Isensee et al. (2018. J. Cell Biol. https://doi.org/10.1083/jcb.201708053) use antibody tools that selectively recognize phosphorylated RII and the catalytic subunit active site to reexamine PKA holoenzyme activation mechanisms in neurons.
May 17, 2018: Journal of Cell Biology
https://www.readbyqxmd.com/read/29772775/ph-responsive-mercaptoundecanoic-acid-functionalized-gold-nanoparticles-and-applications-in-catalysis
#9
Siyam M Ansar, Saptarshi Chakraborty, Christopher L Kitchens
Mercaptoundecanoic acid (MUA) functionalized gold nanoparticles (AuNP-MUA) were synthesized and demonstrated to possess pH-triggered aggregation and re-dispersion, as well as the capability of phase transfer between aqueous and organic phases in response to changes in pH. The pH of aggregation for AuNP-MUA is consistent with the pKa of MUA (pH ~4) in solution, while AuNP-MUA phase transition between aqueous and organic phases occurs at pH ~9. The ion pair formation between the amine group in octadecylamine (ODA), the carboxylate group in MUA, and the hydrophobic alkyl chain of ODA facilitates the phase transfer of AuNP-MUA into an organic medium...
May 17, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29771522/hydronium-ions-accompanying-buried-acidic-residues-lead-to-high-apparent-dielectric-constants-in-the-interior-of-proteins
#10
Xiongwu Wu, Bernard R Brooks
Internal ionizable groups are known to play important roles in protein functions. A mystery that has attracted decades of extensive experimental and theoretical studies is the apparent dielectric constants experienced by buried ionizable groups, which are much higher than values expected for protein interiors. Many interpretations have been proposed, such as water penetration, conformational relaxation, local unfolding, protein intrinsic backbone fluctuations, etc. However, these interpretations conflict with many experimental observations...
May 17, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29771499/guanine-and-8-azaguanine-in-anomeric-dna-hybrid-base-pairs-stability-fluorescence-sensing-and-efficient-mismatch-discrimination-with-%C3%AE-d-nucleosides
#11
Jiang Liu, Sachin Asaram Ingale, Frank Seela
The α-anomers of 8-aza-2'-deoxyguanosine (αGd*) and 2'-deoxyguanosine (αGd) were site-specifically incorporated in 12-mer duplexes opposite to the four canonical DNA constituents dA, dG, dT and dC. Oligodeoxyribonucleotides containing αGd* display significant fluorescence at slightly elevated pH (8.0). Oligodeoxyribonucleotides incorporating β-anomeric 8-aza-2'-deoxyguanosine (Gd*) and canonical dG were studied for comparison. For αGd* synthesis, an efficient purification of anomeric 8-azaguanine nucleosides was developed on the basis of protected intermediates and a new αGd* phosphoramidite was prepared...
May 17, 2018: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29771135/electromembrane-extraction-of-substances-with-weakly-basic-properties-a-fundamental-study-with-benzodiazepines
#12
Linda Vårdal, Elisabeth L Øiestad, Astrid Gjelstad, Stig Pedersen-Bjergaard
AIM: Electromembrane extraction (EME) of weakly basic benzodiazepines was investigated (-1.47 < pKa < 5.01). MATERIALS & METHODS: 96-well EME was performed with strongly acidic conditions in the acceptor solution using 250-mM trifluoroacetic acid to maximize ionization. RESULTS & CONCLUSION: Recoveries more than 80% were obtained for analytes with pKa > 2, whereas EME was less efficient for substances with pKa < 2...
May 17, 2018: Bioanalysis
https://www.readbyqxmd.com/read/29769318/structural-basis-for-selective-inhibition-of-human-pkg-i%C3%AE-by-the-balanol-like-compound-n46
#13
Liying Qin, Banumathi Sankaran, Sahar Aminzai, Darren Casteel, Choel Kim
Activation of PKG Iα in nociceptive neurons induces a long-term hyperexcitability that causes chronic pain. Recently, a derivative of the fungal metabolite balanol, N46, has been reported to inhibit PKG Iα with high potency and selectivity and attenuates thermal hyperalgesia and osteoarthritic pain. Here, we determined co-crystal structures of the PKG Iα C-domain and cAMP-dependent protein kinase (PKA) Cα, each bound with N46, at 1.98 Å and 2.65 Å, respectively. N46 binds the active site with its external phenyl ring specifically interacting with the glycine-rich loop and the αC helix...
May 16, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29768523/extracellular-calcium-increases-fibroblast-growth-factor-2-gene-expression-via-extracellular-signal-regulated-kinase-1-2-and-protein-kinase-a-signaling-in-mouse-dental-papilla-cells
#14
Sousuke Kanaya, Binlu Xiao, Yukihiko Sakisaka, Mizuki Suto, Kentaro Maruyama, Masahiro Saito, Eiji Nemoto
We previously reported that elevated extracellular calcium (Ca2+) levels increase bone morphogenetic protein 2 expression in human dental pulp (hDP) cells. However, it is unknown whether extracellular Ca2+ affects the expression of other growth factors such as fibroblast growth factor 2 (FGF2). The present study aimed to examine the effect of extracellular Ca2+ on FGF2 gene expression in hDP and immortalized mouse dental papilla (mDP) cells. Cells were stimulated with 10 mM CaCl2 in the presence or absence of cell signaling inhibitors...
May 14, 2018: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/29768196/loss-of-abhd15-impairs-the-anti-lipolytic-action-of-insulin-by-altering-pde3b-stability-and-contributes-to-insulin-resistance
#15
Wenmin Xia, Ariane R Pessentheiner, Dina C Hofer, Melina Amor, Renate Schreiber, Gabriele Schoiswohl, Thomas O Eichmann, Evelyn Walenta, Bianca Itariu, Gerhard Prager, Hubert Hackl, Thomas Stulnig, Dagmar Kratky, Thomas Rülicke, Juliane G Bogner-Strauss
Elevated circulating fatty acids (FAs) contribute to obesity-associated metabolic complications, but the mechanisms by which insulin suppresses lipolysis are poorly understood. We show that α/β-hydrolase domain-containing 15 (ABHD15) is required for the anti-lipolytic action of insulin in white adipose tissue (WAT). Neither insulin nor glucose treatments can suppress FA mobilization in global and conditional Abhd15-knockout (KO) mice. Accordingly, insulin signaling is impaired in Abhd15-KO adipocytes, as indicated by reduced AKT phosphorylation, glucose uptake, and de novo lipogenesis...
May 15, 2018: Cell Reports
https://www.readbyqxmd.com/read/29767422/reduction-triggered-transformation-of-crosslinking-modules-of-disulfide-containing-micelles-with-chemically-tunable-rates
#16
Zhengyu Deng, Shuai Yuan, Ronald X Xu, Haojun Liang, Shiyong Liu
A dilemma exists between the circulation stability and cargo release/mass diffusion at desired sites for designing delivery nanocarriers and in vivo nanoreactors. We herein report disulfide-crosslinked (DCL) micelles exhibiting reduction-triggered switching of crosslinking modules and synchronized hydrophobic-to-hydrophilic transition. Tumor cell-targeted DCL micelles undergo cytoplasmic milieu-triggered disulfide cleavage and cascade self-immolative decaging reactions at chemically adjustable rates, generating primary amine moieties...
May 16, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29767301/capillary-electrophoresis-tandem-mass-spectrometry-for-multiclass-analysis-of-polar-marine-toxins
#17
Daniel G Beach, Elliott S Kerrin, Krista Thomas, Michael A Quilliam, Pearse McCarron
Polar marine toxins are more challenging to analyze by mass spectrometry-based methods than lipophilic marine toxins, which are now routinely measured in shellfish by multiclass reversed-phase liquid chromatography-tandem mass spectrometry (MS/MS) methods. Capillary electrophoresis (CE)-MS/MS is a technique that is well suited for the analysis of polar marine toxins, and has the potential of providing very high resolution separation. Here, we present a CE-MS/MS method developed, with use of a custom-built interface, for the sensitive multiclass analysis of paralytic shellfish toxins, tetrodotoxins, and domoic acid in seafood...
May 16, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29760875/glycogen-synthase-kinase-3-promotes-multicellular-development-over-unicellular-encystation-in-encysting-dictyostelia
#18
Yoshinori Kawabe, Takahiro Morio, Yoshimasa Tanaka, Pauline Schaap
Background: Glycogen synthase kinase 3 (GSK3) regulates many cell fate decisions in animal development. In multicellular structures of the group 4 dictyostelid Dictyostelium discoideum , GSK3 promotes spore over stalk-like differentiation. We investigated whether, similar to other sporulation-inducing genes such as cAMP-dependent protein kinase (PKA), this role of GSK3 is derived from an ancestral role in encystation of unicellular amoebas. Results: We deleted GSK3 in Polysphondylium pallidum , a group 2 dictyostelid which has retained encystation as an alternative survival strategy...
2018: EvoDevo
https://www.readbyqxmd.com/read/29758256/reduction-of-protein-kinase-a-mediated-phosphorylation-of-atxn1-s776-in-purkinje-cells-delays-onset-of-ataxia-in-a-sca1-mouse-model
#19
Judit M Pérez Ortiz, Nissa Mollema, Nicholas Toker, Carolyn J Adamski, Brennon O'Callaghan, Lisa Duvick, Jillian Friedrich, Michael A Walters, Jessica Strasser, Jon E Hawkinson, Huda Y Zoghbi, Christine Henzler, Harry T Orr, Sarita Lagalwar
Spinocerebellar ataxia type 1 (SCA1) is a polyglutamine (polyQ) repeat neurodegenerative disease in which a primary site of pathogenesis are cerebellar Purkinje cells. In addition to polyQ expansion of ataxin-1 protein (ATXN1), phosphorylation of ATXN1 at the serine 776 residue (ATXN1-pS776) plays a significant role in protein toxicity. Utilizing a biochemical approach, pharmacological agents and cell-based assays, including SCA1 patient iPSC-derived neurons, we examine the role of Protein Kinase A (PKA) as an effector of ATXN1-S776 phosphorylation...
May 11, 2018: Neurobiology of Disease
https://www.readbyqxmd.com/read/29758169/nutritional-recovery-from-low-protein-diet-during-pregnancy-does-not-restore-the-kinetics-of-insulin-secretion-and-ca2-or-alterations-in-the-camp-pka-and-plc-pkc-pathways-in-islets-from-adult-rats
#20
Leonardo Marin, Hellen Barbosa Farias Silva, Gabriela Damin, Leticia Martins Ignacio-Souza, Silvia Regina de Lima Reis, Camila Aparecida Machado de Oliveira, Rosane Aparecida Ribeiro, Marise A Reis, Marcia Queiroz Latorraca, Fabiano Ferreira, Vanessa Cristina Arantes
We investigated the insulin release induced by glucose, the Ca2+ oscillatory pattern and the cAMP/PKA and PLC/PKC pathways in islets from adult rats that were reared under diets with 17% protein (C) or 6% protein (LP) during gestation, suckling and after weaning and in rats receiving diets with 6% protein during gestation, followed by a 17% protein diet after birth (R). Glucose-induced first-phase insulin secretion was reduced in LP and R islets, and the second phase was partially restored in the R group. Glucose stimulation did not modify [Ca2+]i, but it reduced the [Ca2+] oscillatory frequency in the R group compared to the C group...
May 14, 2018: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
keyword
keyword
25470
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"