keyword
MENU ▼
Read by QxMD icon Read
search

Pat1

keyword
https://www.readbyqxmd.com/read/28795233/bicarbonate-transport-during-enamel-maturation
#1
REVIEW
Kaifeng Yin, Michael L Paine
Amelogenesis (tooth enamel formation) is a biomineralization process consisting primarily of two stages (secretory stage and maturation stage) with unique features. During the secretory stage, the inner epithelium of the enamel organ (i.e., the ameloblast cells) synthesizes and secretes enamel matrix proteins (EMPs) into the enamel space. The protein-rich enamel matrix forms a highly organized architecture in a pH-neutral microenvironment. As amelogenesis transitions to maturation stage, EMPs are degraded and internalized by ameloblasts through endosomal-lysosomal pathways...
August 9, 2017: Calcified Tissue International
https://www.readbyqxmd.com/read/28768202/dual-rna-processing-roles-of-pat1b-via-cytoplasmic-lsm1-7-and-nuclear-lsm2-8-complexes
#2
Caroline Vindry, Aline Marnef, Helen Broomhead, Laure Twyffels, Sevim Ozgur, Georg Stoecklin, Miriam Llorian, Christopher W Smith, Juan Mata, Dominique Weil, Nancy Standart
Pat1 RNA-binding proteins, enriched in processing bodies (P bodies), are key players in cytoplasmic 5' to 3' mRNA decay, activating decapping of mRNA in complex with the Lsm1-7 heptamer. Using co-immunoprecipitation and immunofluorescence approaches coupled with RNAi, we provide evidence for a nuclear complex of Pat1b with the Lsm2-8 heptamer, which binds to the spliceosomal U6 small nuclear RNA (snRNA). Furthermore, we establish the set of interactions connecting Pat1b/Lsm2-8/U6 snRNA/SART3 and additional U4/U6...
August 1, 2017: Cell Reports
https://www.readbyqxmd.com/read/28733417/analysis-of-schizosaccharomyces-pombe-meiosis
#3
Akira Yamashita, Takeshi Sakuno, Yoshinori Watanabe, Masayuki Yamamoto
Meiosis is a specialized cell cycle that generates haploid gametes from diploid cells. The fission yeast Schizosaccharomyces pombe is one of the best model organisms for studying the regulatory mechanisms of meiosis. S. pombe cells, which normally grow in the haploid state, diploidize by conjugation and initiate meiosis when starved for nutrients, especially nitrogen. Following two rounds of chromosome segregation, spore formation takes place. The switch from mitosis to meiosis is controlled by a kinase, Pat1, and an RNA-binding protein, Mei2...
July 21, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28733399/synchronous-induction-of-meiosis-in-the-fission-yeast-schizosaccharomyces-pombe
#4
Akira Yamashita, Takeshi Sakuno, Yoshinori Watanabe, Masayuki Yamamoto
In fission yeast Schizosaccharomyces pombe, initiation of meiosis is repressed by Pat1 kinase. This protocol describes how ectopic inactivation of the temperature-sensitive Pat1-114 kinase in G1-arrested h(-)/h(-) diploid cells carrying mat1-Pc induces a highly synchronized commitment to and execution of meiosis. Haploid or diploid pat1-114 mutants without mat1-Pc can also be used for convenience, although less synchrony may be attained compared with induction using true diploids. An essentially identical protocol can be used for induction via inhibition of genetically sensitized Pat1 kinase by ATP analogs...
July 21, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28603468/minimal-dose-of-milk-protein-concentrate-to-enhance-the-anabolic-signalling-response-to-a-single-bout-of-resistance-exercise-a-randomised-controlled-trial
#5
Cameron J Mitchell, Nina Zeng, Randall F D'Souza, Sarah M Mitchell, Kirsten Aasen, Aaron C Fanning, Sally D Poppitt, David Cameron-Smith
BACKGROUND: Resistance training is a potent stimulus to induce muscle hypertrophy. Supplemental protein intake is known to enhance gains in muscle mass through activation of the mammalian target of rapamycin complex 1 (mTORC1) pathway, which initiates protein translation. While the optimal dose of high quality protein to promote post exercise anabolism in young or older men has been investigated, little is known about the minimum doses of protein required to potentiate the resistance exercise activation of anabolic signalling in middle aged men...
2017: Journal of the International Society of Sports Nutrition
https://www.readbyqxmd.com/read/28526688/loss-of-the-anion-exchanger-dra-slc26a3-or-pat1-slc26a6-alters-sulfate-transport-by-the-distal-ileum-and-overall-sulfate-homeostasis
#6
Jonathan M Whittamore, Marguerite Hatch
The ileum is considered the primary site of inorganic sulfate ([Formula: see text]) absorption. In the present study, we explored the contributions of the apical chloride/bicarbonate (Cl(-)/[Formula: see text]) exchangers downregulated in adenoma (DRA; Slc26a3), and putative anion transporter 1 (PAT1; Slc26a6), to the underlying transport mechanism. Transepithelial (35)[Formula: see text] and (36)Cl(-) fluxes were determined across isolated, short-circuited segments of the distal ileum from wild-type (WT), DRA-knockout (KO), and PAT1-KO mice, together with measurements of urine and plasma sulfate...
September 1, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/28476936/genome-wide-screen-for-cell-growth-regulators-in-fission-yeast
#7
Louise Weston, Jessica Greenwood, Paul Nurse
Cellular growth control is important for all living organisms, but experimental investigation into this problem is difficult because of the complex range of growth regulatory mechanisms. Here, we have used the fission yeast Schizosaccharomyces pombe to identify potential master regulators of growth. At the restrictive temperature, the S. pombe pat1(ts) mei4Δ strain enters the meiotic developmental program, but arrests in meiotic G2 phase as mei4(+) is essential for meiotic progression. These cells do not grow, even in an abundance of nutrients...
June 15, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28430716/simultaneous-multislice-accelerated-turbo-spin-echo-magnetic-resonance-imaging-comparison-and-combination-with-in-plane-parallel-imaging-acceleration-for-high-resolution-magnetic-resonance-imaging-of-the-knee
#8
Jan Fritz, Benjamin Fritz, Jialu Zhang, Gaurav K Thawait, Dharmdev H Joshi, Li Pan, Dingxin Wang
PURPOSE: We prospectively quantified the signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) of integrated parallel acquisition technique (PAT) and simultaneous multislice (SMS) acceleration and various combinations thereof, and we further compared two 4-fold-accelerated (PAT2-SMS2) high-resolution turbo spin echo (TSE) magnetic resonance imaging (MRI) protocols of the knee against a clinical 2-fold-accelerated (PAT2-SMS1) TSE standard. MATERIALS AND METHODS: Institutional review board approval was obtained, and all subjects gave informed consent...
April 20, 2017: Investigative Radiology
https://www.readbyqxmd.com/read/28325844/the-sole-lsm-complex-in-cyanidioschyzon-merolae-associates-with-pre-mrna-splicing-and-mrna-degradation-factors
#9
Kirsten A Reimer, Martha R Stark, Lisbeth-Carolina Aguilar, Sierra R Stark, Robert D Burke, Jack Moore, Richard P Fahlman, Calvin K Yip, Haruko Kuroiwa, Marlene Oeffinger, Stephen D Rader
Proteins of the Sm and Sm-like (LSm) families, referred to collectively as (L)Sm proteins, are found in all three domains of life and are known to promote a variety of RNA processes such as base-pair formation, unwinding, RNA degradation, and RNA stabilization. In eukaryotes, (L)Sm proteins have been studied, inter alia, for their role in pre-mRNA splicing. In many organisms, the LSm proteins form two distinct complexes, one consisting of LSm1-7 that is involved in mRNA degradation in the cytoplasm, and the other consisting of LSm2-8 that binds spliceosomal U6 snRNA in the nucleus...
June 2017: RNA
https://www.readbyqxmd.com/read/28241728/ampk-%C3%AE-ketoglutarate-axis-regulates-intestinal-water-and-ion-homeostasis-in-young-pigs
#10
Liuqin He, Niu Huang, Huan Li, Junquan Tian, Xihong Zhou, Tiejun Li, Kang Yao, Guoyao Wu, Yulong Yin
Water and ion absorption via sensitive aquaporins (AQPs) and ion channels is of critical importance in intestinal health. However, whether α-ketoglutarate (AKG) could improve intestinal water and ion homeostasis in lipopolysaccharide (LPS)-challenged piglets and whether the AMP-activated protein kinase (AMPK) pathway is involved remains largely unknown. This study was conducted to investigate the effect of dietary AKG supplementation on the small intestinal water and ion homeostasis through modulating the AMPK pathway in a piglet diarrhea model...
March 22, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28157726/twenty-four-weeks-of-%C3%AE-alanine-supplementation-on-carnosine-content-related-genes-and-exercise
#11
RANDOMIZED CONTROLLED TRIAL
Bryan Saunders, Vitor DE Salles Painelli, Luana Farias DE Oliveira, Vinicius DA Eira Silva, Rafael Pires DA Silva, Luiz Riani, Mariana Franchi, Lívia DE Souza Gonçalves, Roger Charles Harris, Hamilton Roschel, Guilherme Giannini Artioli, Craig Sale, Bruno Gualano
INTRODUCTION: Skeletal muscle carnosine content can be increased through β-alanine (BA) supplementation, but the maximum increase achievable with supplementation is unknown. No study has investigated the effects of prolonged supplementation on carnosine-related genes or exercise capacity. PURPOSE: This study aimed to investigate the effects of 24 wk of BA supplementation on muscle carnosine content, gene expression, and high-intensity cycling capacity (CCT110%)...
May 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/28031482/involvement-of-fission-yeast-pdc2-in-rna-degradation-and-p-body-function
#12
Chun-Yu Wang, Yi-Ting Wang, Wan-Yi Hsiao, Shao-Win Wang
In this study we identified Pdc2, the fission yeast ortholog of human Pat1b protein, which forms a complex with Lsm1-7 and plays a role in coupling deadenylation and decapping. The involvement of Pdc2 in RNA degradation and P-body function was also determined. We found that Pdc2 interacts with Dcp2 and is required for decapping in vivo. Although not absolutely essential for P-body assembly, overexpression of Pdc2 enhanced P-body formation even in the absence of Pdc1, the fission yeast functional homolog of human Edc4 protein, indicating that Pdc2 also plays a role in P-body formation...
April 2017: RNA
https://www.readbyqxmd.com/read/27797356/the-heterodimeric-transcription-factor-complex-erf115-pat1-grants-regeneration-competence
#13
Jefri Heyman, Toon Cools, Balkan Canher, Sviatlana Shavialenka, Jan Traas, Ilse Vercauteren, Hilde Van den Daele, Geert Persiau, Geert De Jaeger, Keiko Sugimoto, Lieven De Veylder
Regeneration of a tissue damaged by injury represents a physiological response for organ recovery(1-3). Although this regeneration process is conserved across multicellular taxa, plants appear to display extremely high regenerative capacities, a feature widely used in tissue culture for clonal propagation and grafting(4,5). Regenerated cells arise predominantly from pre-existing populations of division-competent cells(6,7); however, the mechanisms by which these cells are triggered to divide in response to injury remain largely elusive(8)...
October 31, 2016: Nature Plants
https://www.readbyqxmd.com/read/27725937/rapid-and-quantitative-detection-of-leifsonia-xyli-subsp-xyli-in-sugarcane-stalk-juice-using-a-real-time-fluorescent-taqman-pcr-assay
#14
Hua-Ying Fu, Sheng-Ren Sun, Jin-Da Wang, Kashif Ahmad, Heng-Bo Wang, Ru-Kai Chen, San-Ji Gao
Ratoon stunting disease (RSD) of sugarcane, one of the most important diseases seriously affecting the productivity of sugarcane crops, was caused by the bacterial agent Leifsonia xyli subsp. xyli (Lxx). A TaqMan probe-based real-time quantitative polymerase chain reaction (qPCR) assay was established in this study for the quantification of Lxx detection in sugarcane stalk juice. A pair of PCR primers (Pat1-QF/Pat1-QR) and a fluorogenic probe (Pat1-QP) targeting the Part1 gene of Lxx were used for the qPCR assay...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27706767/bioinformatic-analyses-of-gras-genes-in-betula-kirghisorum-based-on-transcriptome-data
#15
C J Yang, G Y Li, Y L Cui
The transcriptomes of salt-stressed and unstressed Betula kirghisorum plants were analyzed using high throughput sequencing technology. A total of 52,239,804 and 51,772,998 clean reads were obtained from the two libraries, respectively, and de novo assembled into 60,545 all-unigenes. A total of 39,997 unigenes were annotated using public databases. Overall, 7206 genes were differentially expressed in unigenes and were involved in 127 pathways. Thirteen transcription factor families were identified in B. kirghisorum, including GRAS proteins, which are plant-specific transcription factors...
September 23, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27694841/structure-of-the-active-form-of-dcp1-dcp2-decapping-enzyme-bound-to-m-7-gdp-and-its-edc3-activator
#16
Clément Charenton, Valerio Taverniti, Claudine Gaudon-Plesse, Régis Back, Bertrand Séraphin, Marc Graille
Elimination of the 5' cap of eukaryotic mRNAs, known as decapping, is considered to be a crucial, irreversible and highly regulated step required for the rapid degradation of mRNA by Xrn1, the major cytoplasmic 5'-3' exonuclease. Decapping is accomplished by the recruitment of a protein complex formed by the Dcp2 catalytic subunit and its Dcp1 cofactor. However, this complex has a low intrinsic enzymatic activity and requires several accessory proteins such as the Lsm1-7 complex, Pat1, Edc1-Edc2 and/or Edc3 to be fully active...
November 2016: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/27690010/recent-advances-in-understanding-amino-acid-sensing-mechanisms-that-regulate-mtorc1
#17
REVIEW
Liufeng Zheng, Wei Zhang, Yuanfei Zhou, Fengna Li, Hongkui Wei, Jian Peng
The mammalian target of rapamycin (mTOR) is the central regulator of mammalian cell growth, and is essential for the formation of two structurally and functionally distinct complexes: mTORC1 and mTORC2. mTORC1 can sense multiple cues such as nutrients, energy status, growth factors and hormones to control cell growth and proliferation, angiogenesis, autophagy, and metabolism. As one of the key environmental stimuli, amino acids (AAs), especially leucine, glutamine and arginine, play a crucial role in mTORC1 activation, but where and how AAs are sensed and signal to mTORC1 are not fully understood...
September 29, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27635351/genome-wide-identification-and-characterization-of-gras-transcription-factors-in-sacred-lotus-nelumbo-nucifera
#18
Yu Wang, Shenglu Shi, Ying Zhou, Yu Zhou, Jie Yang, Xiaoqing Tang
The GRAS gene family is one of the most important plant-specific gene families, which encodes transcriptional regulators and plays an essential role in plant development and physiological processes. The GRAS gene family has been well characterized in many higher plants such as Arabidopsis, rice, Chinese cabbage, tomato and tobacco. In this study, we identified 38 GRAS genes in sacred lotus (Nelumbo nucifera), analyzed their physical and chemical characteristics and performed phylogenetic analysis using the GRAS genes from eight representative plant species to show the evolution of GRAS genes in Planta...
2016: PeerJ
https://www.readbyqxmd.com/read/27434131/mutagenic-analysis-of-the-c-terminal-extension-of-lsm1
#19
Ashis Chowdhury, Swathi Kalurupalle, Sundaresan Tharun
The Sm-like proteins (also known as Lsm proteins) are ubiquitous in nature and exist as hexa or heptameric RNA binding complexes. They are characterized by the presence of the Sm-domain. The Lsm1 through Lsm7 proteins are highly conserved in eukaryotes and they form a hetero-octameric complex together with the protein Pat1. The Lsm1-7-Pat1 complex plays a key role in mRNA decapping and 3'-end protection and therefore is required for normal mRNA decay rates in vivo. Lsm1 is a key subunit that is critical for the unique RNA binding properties of this complex...
2016: PloS One
https://www.readbyqxmd.com/read/27354705/the-s-pombe-mrna-decapping-complex-recruits-cofactors-and-an-edc1-like-activator-through-a-single-dynamic-surface
#20
Jan Philip Wurm, Jan Overbeck, Remco Sprangers
The removal of the 5' 7-methylguanosine mRNA cap structure (decapping) is a central step in the 5'-3' mRNA degradation pathway and is performed by the Dcp1:Dcp2 decapping complex. The activity of this complex is tightly regulated to prevent premature degradation of the transcript. Here, we establish that the aromatic groove of the EVH1 domain of Schizosaccharomyces pombe Dcp1 can interact with proline-rich sequences in the exonuclease Xrn1, the scaffolding protein Pat1, the helicase Dhh1, and the C-terminal disordered region of Dcp2...
September 2016: RNA
keyword
keyword
25423
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"