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Oleksandr H Minchenko, Dariia O Tsymbal, Dmytro O Minchenko, Svitlana V Prylutska, Oksana S Hnatiuk, Yuriy I Prylutskyy, Nikos G Tsierkezos, Uwe Ritter
The effect of single-walled carbon nanotubes (SWCNTs) on the expression of a subset of immune response, apoptosis and cell proliferation -associated genes was studied in normal human astrocytes (line NHA/TS). In the cells treated with SWCNTs (2, 10 and 50 ng/ml of medium for 24 h) we observed a strong dose-dependent down-regulation of the expression of a cell surface glycoproteins HLA-DRA (major histocompatibility complex, class II, DR alpha) and HLA-DRB1. At the same time, the expression of HLA-F (major histocompatibility complex, class I, F), LMNB1 (lamin B1), and HTRA1 (high temperature requirement A1) genes as well as the level of miR-190b and miR-7 was up-regulated in NHA/TS subjected to different concentrations of SWCNTs...
June 12, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Ho-Su Lee, Suk-Kyun Yang, Myunghee Hong, Seulgi Jung, Byoung Mok Kim, Jung Won Moon, Sang Hyoung Park, Byong Duk Ye, Seak Hee Oh, Kyung Mo Kim, Yong Sik Yoon, Chang Sik Yu, Jiwon Baek, Cue Hyunkyu Lee, Buhm Han, Jianjun Liu, Talin Haritunians, Dermot P B McGovern, Kyuyoung Song
Background and Aims: The genetic contribution to the prognosis of ulcerative colitis (UC) is poorly understood, and most currently known susceptibility loci are not associated with prognosis. To identify genetic variants influencing the prognosis of UC, we performed an Immunochip-based study using an extreme phenotype approach. Methods: Based on the finding that the only association Pdiscovery-meta < 1 × 10-4 was located in the human leukocyte antigen (HLA), we focused our analyses on the HLA region...
June 13, 2018: Journal of Crohn's & Colitis
Shuzo Kaneko, Kunihiro Yamagata
Accumulation of amyloid fibrils from β2-microglobulin (β2M) was first recognized as a characteristic osteoarticular complication in long-term hemodialysis (HD) patients and called "HD-related amyloidosis" (HRA). However, this syndrome can also be observed in end-stage renal diseases (ESRD) patients undergoing peritoneal dialysis, and even in patients with chronic renal failure before the initiation of dialytic therapy, suggesting that HD is not a direct cause but that accumulation of β2M or some β2M-associated molecules in the body is a common pathogenesis...
June 12, 2018: Seminars in Dialysis
Suguru Yamamoto
Uremic toxins are linked to chronic kidney disease (CKD)-related systemic diseases. β2 -Microglobulin (β2 -m), a water-soluble, middle-sized molecule, is associated with mortality and dialysis-related amyloidosis (DRA). DRA occurs in long-term dialysis patients, with β2 -m amyloid deposited mainly in osteoarticular tissues. We investigated a model of β2 -m amyloid fibril extension at neutral pH in the presence of trifluoroethanol or sodium dodecyl sulfate. Using this model, some biological molecules, including glycosaminoglycans and lysophospholipids, were found to be chaperones for β2 -m amyloid fibril extension...
June 5, 2018: Clinical and Experimental Nephrology
Markus D Lacher, Gerhard Bauer, Brian Fury, Sanne Graeve, Emily L Fledderman, Tye D Petrie, Dane P Coleal-Bergum, Tia Hackett, Nicholas H Perotti, Ying Y Kong, William W Kwok, Joseph P Wagner, Charles L Wiseman, William V Williams
Targeted cancer immunotherapy with irradiated, granulocyte-macrophage colony-stimulating factor (GM-CSF)-secreting, allogeneic cancer cell lines has been an effective approach to reduce tumor burden in several patients. It is generally assumed that to be effective, these cell lines need to express immunogenic antigens coexpressed in patient tumor cells, and antigen-presenting cells need to take up such antigens then present them to patient T cells. We have previously reported that, in a phase I pilot study (ClinicalTrials...
2018: Frontiers in Immunology
Abdelaziz Ed-Dra, Bouchra Karraouan, Abdellah El Allaoui, Meriem Khayatti, Hicham El Ossmani, Fouzia Rhazi Filali, Naima ElMdaghri, Brahim Bouchrif
OBJECTIVES: Genotyping of Salmonella strains is an important molecular tool to discriminate the isolates and to improve the epidemiological studies when an outbreak occurs. Among the genotyping DNA-based methods, pulsed field gel electrophoresis (PFGE) is currently in use to subtype Salmonella isolates. In this study, we evaluated the feasibility of genotyping Salmonella Infantis strains by XbaI restriction enzyme. Separation of restricted fragments was performed by PFGE. METHODS: In order to test the possibility to apply this methodology to epidemiological investigation, a collection of 26 Salmonella Infantis strains were tested for their susceptibility to 14 antibiotics and analyzed using XbaI macro-restriction followed by PFGE...
May 26, 2018: Journal of Global Antimicrobial Resistance
Cindy Im, Yadav Sapkota, Wonjong Moon, Minae Kawashima, Minoru Nakamura, Katsushi Tokunaga, Yutaka Yasui
Primary biliary cholangitis (PBC) susceptibility loci have largely been discovered through single SNP association testing. In this study, we report genic haplotype patterns associated with PBC risk genome-wide in two Japanese cohorts. Among the 74 genic PBC risk haplotype candidates we detected with a novel methodological approach in a discovery cohort of 1,937 Japanese, nearly two-thirds were replicated (49 haplotypes, Bonferroni-corrected P < 6.8 × 10-4 ) in an independent Japanese cohort (N = 949)...
May 17, 2018: Scientific Reports
Abdellah Aghraz, Qada Benameur, Teresa Gervasi, Loubna Ait Dra, Meriem Hind Ben-Mahdi, Mustapha Larhsini, Mohamed Markouk, Nicola Cicero
Multidrug-resistant bacteria have become common all over the world, necessitating the development of new therapeutic strategies. Synergistic interactions between conventional antibiotics and natural bioactive may have therapeutic benefits in a clinical setting. There are plenty of medicinal plants that have proven efficacy against broad spectrum of microorganisms. The aim of the work was to assess the antibacterial activity of Cladanthus arabicus, a Moroccan medicinal plant, and Bubonium imbricatum, a Moroccan endemic plant...
May 15, 2018: Letters in Applied Microbiology
Yue Pan, Lingyun Lu, Junquan Chen, Yong Zhong, Zhehao Dai
Background: This study aimed to identify potential crucial genes and construction of microRNA-mRNA negative regulatory networks in osteosarcoma by comprehensive bioinformatics analysis. Methods: Data of gene expression profiles (GSE28424) and miRNA expression profiles (GSE28423) were downloaded from GEO database. The differentially expressed genes (DEGs) and miRNAs (DEMIs) were obtained by R Bioconductor packages. Functional and enrichment analyses of selected genes were performed using DAVID database...
2018: Hereditas
Verónica Y Burgos-Elías, Ma José Marroquín-Sarti, Martin A Zimmermann-Paiz, Ana Marissa Ordoñez Rivas, Nancy C Quezada-Del Cid
of this study was to determine the characteristics of patients younger than 14 years who underwent traumatic cataract surgery at the Unit of Pediatric Ophthalmology, Strabismus and Neuro-Ophthalmology "Dra. Ana María Illescas Putzeys" of Hospital de Ojos y Oídos "Dr. Rodolfo Robles V." A retrospective, descriptive, and observational study was carried out to review the medical records of patients who underwent cataract surgery between 2010 and 2015. A total of 54 children underwent traumatic cataract surgery; 75% were boys; 68% were in the 6-11-year-old age range...
June 1, 2018: Archivos Argentinos de Pediatría
Chang Mo Moon, Seung Won Kim, Jae Bum Ahn, Hyun Woo Ma, Xiumei Che, Tae Il Kim, Won Ho Kim, Jae Hee Cheon
Background: Genome-wide association studies and meta-analyses have revealed the genetic background of ulcerative colitis (UC) by identifying common variants. However, these variants do not fully explain the disease variance in UC. To identify novel variants, we performed deep resequencing of UC-associated genes in Korean UC patients and subsequently investigated the functional roles of identified susceptibility genes. Methods: We performed targeted deep resequencing of 108 genes in 24 Korean UC patients and then performed association analysis with data from 126 healthy controls...
May 4, 2018: Inflammatory Bowel Diseases
Z M Estrada-Reyes, Y Tsukahara, A L Goetsch, T A Gipson, T Sahlu, R Puchala, Z Wang, S P Hart, R G Mateescu
The effect of Ovar-DRA and Ovar-DRB1 genotypes on fecal egg count (FEC) was determined in sheep and goats infected with Haemonchus contortus. One hundred and forty three sheep from 3 different breeds (St. Croix, Katahdin and Dorper) and 150 goats from three different breeds (Spanish, Boer and Kiko) were used. Parasitological (FEC), hematological (packed cell volume) and immunological (IgA, IgG and IgM) parameters were measured. Sheep populations showed a higher FEC and humoral response than goat breeds. Genotypes were determined by High Resolution Melting Assays and by conventional PCR...
May 2, 2018: Parasite Immunology
Xiaosheng Hang, Dapeng Li, Jianping Wang, Ge Wang
The aim of the present study was to reveal the potential molecular mechanisms of microsatellite instability (MSI) on the prognosis of gastric cancer (GC). The investigation was performed based on an RNAseq expression profiling dataset downloaded from The Cancer Genome Atlas, including 64 high‑level MSI (MSI‑H) GC samples, 44 low‑level MSI (MSI‑L) GC samples and 187 stable microsatellite (MSI‑S) GC samples. Differentially expressed genes (DEGs) were identified between the MSI‑H, MSI‑L and MSI‑S samples...
April 26, 2018: International Journal of Molecular Medicine
Andrew R Craig, Paul J Cunningham, Mary M Sweeney, Timothy A Shahan, John A Nevin
Delivery of alternative reinforcers in the presence of stimuli previously associated with reinforcement for target behavior increases the susceptibility of target behavior to relapse. To explore contingencies that might mitigate this counter-therapeutic effect, we trained pigeons on a procedure that entailed extinction of previously reinforced target-key pecking, access to a distinct stimulus context contingently on refraining from target behavior (differential-reinforcement-of-other-behavior; DRO), and reinforcement of alternative-key pecks (differential-reinforcement of alternative behavior; DRA) in that context...
May 2018: Journal of the Experimental Analysis of Behavior
Dra Asunción Hernández-Fernández, Elísabet Mora, María Isabel Vizcaíno Hernández
The dual aim of this research is, firstly, to analyze the physiological and unconscious emotional response of consumers to a new technological product and, secondly, link this emotional response to consumer conscious verbal reports of positive and negative product perceptions. In order to do this, biometrics and self-reported measures of emotional response are combined. On the one hand, a neuromarketing experiment based on the facial recognition of emotions of 10 subjects, when physical attributes and economic information of a technological product are exposed, shows the prevalence of the ambivalent emotion of surprise...
April 17, 2018: Physiology & Behavior
Ke Wan, Jiayu Sun, Dan Yang, Hong Liu, Jie Wang, Wei Cheng, Qing Zhang, Zhi Zeng, Tianjing Zhang, Andreas Greiser, Marie-Pierre Jolly, Yuchi Han, Yucheng Chen
Purpose To measure left ventricular (LV) myocardial strain with cine magnetic resonance (MR) imaging and a deformable registration algorithm (DRA) and to assess the prognostic value of myocardial strain in patients with light-chain (AL) amyloidosis. Materials and Methods In this prospective study, 78 consecutive patients with AL amyloidosis who underwent contrast material-enhanced cardiac MR imaging were enrolled at West China Hospital. LV myocardial strains and late gadolinium enhancement (LGE) were evaluated...
April 17, 2018: Radiology
Mailin Adriana Villán-Villán, Rodrigo Pérez-Rodríguez, Cristina Martín, Patricia Sánchez-González, Ignasi Soriano, Eloy Opisso, Dra M Elena Hernando, José María Tormos, Josep Medina, Enrique J Gómez
BACKGROUND: The increasing number of patients with acquired brain injury and the current subjectivity of the conventional upper extremity (UE) assessment tests require new objective assessment techniques. OBJECTIVE: This research proposes a novel objective motor assessment (OMA) methodology based on the Fugl-Meyer assessment (FMA). The goals are to automatically calculate the objective scores (OSs) of FMA-UE movements (as well as a global OS) and to interpret the estimated OSs...
April 7, 2018: NeuroRehabilitation
Sławomir Kujawski, Agnieszka Kujawska, Małgorzata Gajos, Jacek J Klawe, Małgorzata Tafil-Klawe, Katarzyna Mądra-Gackowska, Błażej Stankiewicz, Julia L Newton, Kornelia Kędziora-Kornatowska, Paweł Zalewski
BACKGROUND: Arterial stiffness (AS) is a reduction in the ability of large arteries to readily accommodate the increase in blood ejected from the heart during systole related with aging. Physical exercise is associated with AS reduction. However, it remains controversial as to which modality and intensity (resistance vs aerobic, high vs low) would be the most effective. The aim of these studies is to examine the effects of 3-months sitting callisthenic balance (SCB) and resistance exercise (RET) on aerobic capacity, aortic stiffness and body composition in older participants...
July 15, 2018: Experimental Gerontology
Tanja Heinloth, Juliane Uhlhorn, Mathias F Wernet
The e-vector orientation of linearly polarized light represents an important visual stimulus for many insects. Especially the detection of polarized skylight by many navigating insect species is known to improve their orientation skills. While great progress has been made towards describing both the anatomy and function of neural circuit elements mediating behaviors related to navigation, relatively little is known about how insects perceive non-celestial polarized light stimuli, like reflections off water, leaves, or shiny body surfaces...
2018: Frontiers in Cellular Neuroscience
Hayley P Coffing, Shubha Priyamvada, Arivarasu Natarajan Anbazhagan, Christine Salibay, Melinda A Engevik, James Versalovic, Mary Beth Yacyshyn, Bruce R Yacyshyn, Sangeeta Tyagi, Seema Saksena, Ravinder K Gill, Waddah A Alrefai, Pradeep K Dudeja
Clostridium difficile infection is the primary cause of nosocomial diarrhea in the United States. While C. difficile toxins A and B are the primary mediators of CDI, the overall pathophysiology underlying C. difficile associated diarrhea remains poorly understood. Studies have shown that both NHE3 (Na+ /H+ exchanger) and DRA (Down Regulated in Adenoma, Cl- /HCO3 - exchanger) resulting in decreased electrolyte absorption are implicated in infectious and inflammatory diarrhea. Furthermore, studies have shown that NHE3 is depleted at the apical surface of intestinal epithelial cells and down-regulated in patients with CDI, but the role of DRA in C...
March 29, 2018: American Journal of Physiology. Gastrointestinal and Liver Physiology
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