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Hua Xu, Fayez K Ghishan, Pawel R Kiela
The Slc9 family of Na+ /H+ exchangers (NHEs) plays a critical role in electroneutral exchange of Na+ and H+ in the mammalian intestine as well as other absorptive and secretory epithelia of digestive organs. These transport proteins contribute to the transepithelial Na+ and water absorption, intracellular pH and cellular volume regulation as well as the electrolyte, acid-base, and fluid volume homeostasis at the systemic level. They also influence the function of other membrane transport mechanisms, affect cellular proliferation and apoptosis as well as cell migration, adherence to the extracellular matrix, and tissue repair...
March 26, 2018: Comprehensive Physiology
Huai-Ren Chang, Chih-Feng Lien, Jing-Ren Jeng, Jen-Che Hsieh, Chen-Wei Chang, Jian-Hong Lin, Kun-Ta Yang
BACKGROUND/AIMS: Intermittent hypoxia (IH) has been shown to exert preconditioning-like cardioprotective effects. It also has been reported that IH preserves intracellular pH (pHi) during ischemia and protects cardiomyocytes against ischemic reperfusion injury. However, the exact mechanism is still unclear. METHODS: In this study, we used proton indicator BCECF-AM to analyze the rate of pHi recovery from acidosis in the IH model of rat neonatal cardiomyocytes. Neonatal cardiomyocytes were first treated with repetitive hypoxia-normoxia cycles for 1-4 days...
April 16, 2018: Cellular Physiology and Biochemistry
Ozgur Kilic, Ellen Kemler, Vincent Gouttebarge
OBJECTIVE: To gather epidemiological information related to all steps of Van Mechelen's "sequence of prevention" for musculoskeletal injuries among adult recreational football players. METHODS: A systematic review of the scientific literature was conducted in Medline via Pubmed. Therefore, two highly sensitive search strategies based on three groups of keywords (and related search terms) were used. RESULTS: In total, 33 relevant original studies were included in our systematic review...
February 2, 2018: Physical Therapy in Sport
Valentina Leandri, Quentin Daniel, Hong Chen, Licheng Sun, James M Gardner, Lars Kloo
The reaction of CuCl2 with 2,9-dimethyl-1,10-phenanthroline (dmp) does not lead to the formation of [Cu(dmp)2 ](Cl)2 but instead to [Cu(dmp)2 Cl]Cl, a 5-coordinated complex, in which one chloride is directly coordinated to the metal center. Attempts at removing the coordinated chloride by changing the counterion by metathesis were unsuccessful and resulted only in the exchange of the noncoordinated chloride, as confirmed from a crystal structure analysis. Complex [Cu(dmp)2 Cl]PF6 exhibits a reversible cyclic voltammogram characterized by a significant peak splitting between the reductive and oxidative waves (0...
April 2, 2018: Inorganic Chemistry
Guocan Li, Matthew David Brady, Gerald J Meyer
The complex [Ru(deeb)(bpz)2]2+ (RuBPZ2+, deeb = 4,4'-diethylester-2,2'-bipyridine, bpz = 2,2'-bipyrazine) forms a single ion pair with bromide, [RuBPZ2+, Br-]+, with Keq = 8400 ± 200 M-1 in acetone. The RuBPZ2+ displayed photoluminescence (PL) at room temperature with a lifetime of 1.75 µs. The addition of bromide to a RuBPZ2+ acetone solution led to significant PL quenching and Stern-Volmer plots showed upward curvature. Time-resolved PL measurements identified two excited state quenching pathways, static and dynamic, which were operative towards [RuBPZ2+, Br-]+ and free RuBPZ2+, respectively...
March 29, 2018: Journal of the American Chemical Society
Samuel M Goodman, Max Levy, Fei-Fei Li, Yuchen Ding, Colleen M Courtney, Partha P Chowdhury, Annette Erbse, Anushree Chatterjee, Prashant Nagpal
The rapid emergence of superbugs, or multi-drug resistant (MDR) organisms, has prompted a search for novel antibiotics, beyond traditional small-molecule therapies. Nanotherapeutics are being investigated as alternatives, and recently superoxide-generating quantum dots (QDs) have been shown as important candidates for selective light-activated therapy, while also potentiating existing antibiotics against MDR superbugs. Their therapeutic action is selective, can be tailored by simply changing their quantum-confined conduction-valence band (CB-VB) positions and alignment with different redox half-reactions-and hence their ability to generate specific radical species in biological media...
2018: Frontiers in Chemistry
Mohammad Mahdi Najafpour, Navid Jameei Moghaddam, Leila Hassani, Robabeh Bagheri, Zhenlun Song, Suleyman I Allakhverdiev
Nature uses a Mn oxide-based catalyst for water oxidation in plants, algae, and cyanobacteria. Mn oxides are among major candidates to be used as water-oxidizing catalysts. Herein, we used two straightforward and promising methods to form Escherichia coli bacteria/Mn oxide compounds. In one of the methods, the bacteria template was intact after the reaction. The catalysts were characterized by X-ray photoelectron spectroscopy, visible spectroscopy, scanning electron microscopy, high-resolution transmission electron microscopy, diffuse reflectance infrared Fourier transform spectroscopy, Raman spectroscopy, and X-ray diffraction spectrometry...
March 27, 2018: Photosynthesis Research
Agata Głuszyńska, Bernard Juskowiak, Martyna Kuta-Siejkowska, Marcin Hoffmann, Shozeb Haider
The interactions of c-myc G-quadruplex with three carbazole derivatives were investigated by UV-Vis spectrophotometry, fluorescence, CD spectroscopy, and molecular modeling. The results showed that a combination of carbazole scaffold functionalized with ethyl, triazole and imidazole groups resulted in stabilization of the intramolecular G-quadruplex formed by the DNA sequence derived from the NHE III1 region of c-myc oncogene (Pu22). Binding to the G-quadruplex Pu22 resulted in the significant increase in fluorescence intensity of complexed ligands 1-3...
March 23, 2018: International Journal of Biological Macromolecules
Xian Lu, Guanqun Wo, Binghua Li, Chun Xu, Junhua Wu, Chunping Jiang, Jiwu Wei
The NF-κB/IL-6/STAT3 inflammatory axis is highly activated in a variety of inflammation-related cancers and contributes to suppression of antitumor immunity. In this study, we generated a novel herbal formula NHE-06, a water-decocting extract from six natural herbals, Ficus carica, Taraxacum mongolicum, Angelica sinensis, Lonicera japonica, Pseudo-ginseng and Folium ginkgo. We investigated the anti-inflammatory properties of NHE-06 and its antitumor efficacy in hepatocellular carcinoma, a typical inflammation-related cancer...
March 22, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Joel D Presland, Ryan G Timmins, Matthew N Bourne, Morgan D Williams, David A Opar
PURPOSE: To determine the time course of architectural adaptations in the biceps femoris long head (BFLH ) following high or low volume eccentric training. METHODS: Twenty recreationally active males completed a two week standardised period of eccentric Nordic hamstring exercise (NHE) training, followed by four weeks of high (n=10) or low volume (n=10) training. Eccentric strength was assessed pre and post intervention and following detraining. Architecture was assessed weekly during training and after two and four weeks of detraining...
March 23, 2018: Scandinavian Journal of Medicine & Science in Sports
Mayuko Nakamura, Yoshichika Ikeda, Akira Matsumoto, Hideaki Maki, Hisayuki Arakawa
As a result of the 2011 Great East Japan earthquake, about 11,000kL of fuel oil was spilled into Kesennuma Bay, Miyagi Prefecture. This oil either accumulated in seabed sediments or was burned in a marine fire on the sea surface. We investigated spatial and temporal variations in the concentrations of oil and hydrocarbons. The maximum concentrations of n-hexane extract (NHE), total petroleum hydrocarbon (TPH), and polycyclic aromatic hydrocarbons (PAHs) in the sediments in 2012 were 8000, 1200, and 16.2mg/kg (DW), respectively...
March 2018: Marine Pollution Bulletin
Rui Xiong, Wenbing Li, Yidan Li, Kaixuan Zheng, Tingting Zhang, Mingyang Gao, Yun Li, Lin Hu, Changjiang Hu
INTRODUCTION: Er Shen Wan (ESW), a traditional Chinese medicinal formula comprised of Psoraleae Fructus (Babchi seeds, from Psoralea corylifolia Linn.) and Myristicae Semen (Nutmeg, from Myristica fragrans Houtt.), is widely used to treat spleen-kidney Yang deficiency (SKYD)-induced diarrhea. Previous studies have demonstrated preliminarily that the petroleum ether extract of ESW (ESWP) exhibits significant anti-diarrheal activity. The present study aimed to evaluate the anti-diarrhea activity of ESWP and to explore the underlying mechanisms with respect to fluid metabolism in a rat model of SKYD-induced diarrhea...
February 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Hari Prasad, Rajini Rao
The pH of the endolysosomal system is tightly regulated by a balance of proton pump and leak mechanisms that are critical for storage, recycling, turnover, and signaling functions in the cell. Dysregulation of endolysosomal pH has been linked to aging, amyloidogenesis, synaptic dysfunction, and various neurodegenerative disorders, including Alzheimer's disease. Therefore, understanding the mechanisms that regulate luminal pH may be key to identifying new targets for managing these disorders. Metaanalysis of yeast microarray databases revealed that nutrient limiting conditions inhibited the histone deacetylase (HDAC) Rpd3 and thereby upregulated transcription of the endosomal Na+ /H+ exchanger Nhx1, resulting in vacuolar alkalinization...
March 22, 2018: Journal of Biological Chemistry
Alison Tebo, Tyler B J Pinter, Ricardo García-Serres, Amy L Speelman, Cédric Tard, Olivier Sénèque, Geneviève Blondin, Jean-Marc Latour, James E Penner-Hahn, Nicolai Lehnert, Vincent L Pecoraro
Protein design is a powerful tool to interrogate the basic requirements for the function of a metal site in a way that allows for the selective incorporation of elements important for function. Rubredoxins are small electron transfer proteins with a reduction potential centered near 0 mV (vs. NHE). All previous attempts at the design of a rubredoxin site have focused on incorporating the canonical CXXC motifs in addition to reproducing the peptide fold or using flexible loop regions to define the morphology of the site...
March 21, 2018: Biochemistry
Jianjun Chang, Yan Ding, Zhiyu Zhou, Hong-Guang Nie, Hong-Long Ji
Transepithelial fluid and salt re-absorption in epithelial tissues play an important role in fluid and salt homeostasis. In absorptive epithelium, fluid and salt flux is controlled by machinery mainly composed of epithelial sodium channels (ENaC), cystic fibrosis transmembrane conductance regulator (CFTR), Na⁺/H⁺ exchanger (NHE), aquaporin, and sodium potassium adenosine triphosphatase (Na⁺/K⁺-ATPase). Dysregulation of fluid and salt transport across epithelium contributes to the pathogenesis of many diseases, such as pulmonary edema and cystic fibrosis...
March 16, 2018: International Journal of Molecular Sciences
Yuan Zhao, Shengbo Zhang, Mengyun Wang, Jinyu Han, Hua Wang, Zhongcheng Li, Xiao Liu
Intensive efforts have been made towards designing water oxidation catalysts using homogeneous metal complexes. However, poor stability, caused by structure collapse and strong oxidants, prohibits further application of these catalysts. Therefore, the fabrication of stable and efficient water oxidation catalysts with robust structures and mild driving forces is in high demand. In this paper, we demonstrate the heterogeneous, porous and stable structure of Ir-doped metal organic frameworks (MOFs) by functionalizing bipyridine-incorporated MOFs with [IrCp*Cl(μ-Cl)]2 complexes...
March 12, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
Gunng-Shinng Chen, Shiao-Pieng Lee, Shu-Fu Huang, Shih-Chi Chao, Chung-Yi Chang, Gwo-Jang Wu, Chung-Hsing Li, Shih-Hurng Loh
OBJECTIVE: Homeostasis of intracellular pH (pHi ) plays vital roles in many cell functions, such as proliferation, apoptosis, differentiation and metastasis. Thus far, Na+ -H+ exchanger (NHE), Na+ -HCO3 - co-transporter (NBC), Cl- /HCO3 - exchanger (AE) and Cl- /OH- exchanger (CHE) have been identified to co-regulate pHi homeostasis. However, functional and biological pHi -regulators in human dental pulp stem cells (hDPSCs) have yet to be identified. DESIGN: Microspectrofluorimetry technique with pH-sensitive fluorescent dye, BCECF, was used to detect pHi changes...
March 6, 2018: Archives of Oral Biology
Ling Zhang, Kasper P Kepp, Jens Ulstrup, Jingdong Zhang
Metalloporphyrins are active sites in metalloproteins and synthetic catalysts. They have also been studied extensively by electrochemistry as well as being prominent targets in electrochemical scanning tunneling microscopy (STM). Previous studies of FePPIX adsorbed on graphite and alkylthiol modified Au electrodes showed a pair of reversible Fe(III/II)PPIX peaks at about -0.41 V (vs. NHE) at high solution pH. We recently used iron protoporphyrin IX (FeP-PIX) as an intercalating probe for long-range electrochemical electron transfer through a G-quadruplex oligonucleo-tide (DNAzyme); this study disclosed two, rather than a single pair of voltammetric peaks with a new and dominating peak, shifted 200 mV positive relative to the  -0...
March 6, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Nasser A M Barakat, Ahmed G El-Deen, Zafar Khan Ghouri, Saeed Al-Meer
NiFe nanoparticles-decorated & N-doped graphene is introduced as an effective and stable non-precious electrocatalyst for ORR in the acid medium. Compared to conventional Pt/C electrodes under the same conditions, the proposed nanocatalyst shows closer onset potential and current density. Typically, the observed onset potentials and current densities for the synthesized and Pt/C electrodes are 825 and 910 mV (vs. NHE) and -3.65 and -4.31 (at 5 mV.s-1 ), respectively. However, the most important advantage of the introduced metallic alloy-decorated graphene is its distinct stability in acid medium; the retention in the electrocatalytic performance after 1,000 successive cycles is approximately 98%...
February 28, 2018: Scientific Reports
Stefania Muzzachi, Lorenzo Guerra, Nicola Antonio Martino, Maria Favia, Giuseppe Punzi, Fabio Silvestre, Antonio Ciro Guaricci, Maria Teresa Roscino, Ciro Leonardo Pierri, Maria Elena Dell'Aquila, Valeria Casavola, Giovanni Michele Lacalandra, Elena Ciani
Sperm motility, a feature essential for in vivo fertilization, is influenced by intracellular pH (pHi ) homeostasis. Several mechanisms are involved in pHi regulation, among which sodium-hydrogen exchangers (NHEs), a family of integral transmembrane proteins that catalyse the exchange of Na+ for H+ across lipid bilayers. A preliminary characterization of NHE activity and kinetic parameters, followed by analysis of the expression and localization of the protein in ram spermatozoa was performed. NHE activity showed an apparent Km for external Na+ of 17...
February 28, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
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