keyword
MENU ▼
Read by QxMD icon Read
search

A

keyword
https://www.readbyqxmd.com/read/28941453/development-of-a-dna-aptamer-for-screening-neisseria-meningitidis-serogroup-b-by-cell-selex
#1
Kimia Mirzakhani, Seyed Latif Mousavi Gargari, Iraj Rasooli, Samaneh Rasoulinejad
Background: Artificial oligonucleotides like DNA or RNA aptamers can be used as biodiagnostic alternatives for antibodies to detect pathogens. Comparing to antibodies, artificial oligonucleotides are produced easily at lower costs and are more stable. Neisseria meningitidis, the causative agent of meningitis, is responsible for about 1% of infections in an epidemic period. Specific DNA aptamers that bind to N. meningitidis serogroup B were identified by whole-cell Systemic Evolution of Ligands by EXponential Enrichment (SELEX)...
September 24, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28941417/protective-role-of-liriodendrin-in-mice-with-dextran-sulphate-sodium-induced-ulcerative-colitis
#2
Zhen Zhang, Lei Yang, Botao Wang, Lanqiu Zhang, Qi Zhang, Dihua Li, Shukun Zhang, Hongwei Gao, Ximo Wang
Sargentodoxa cuneata, containing syringaresinol and its glycoside liriodendrin as the main bioactive compounds, is a well-known traditional Chinese medicine for treating intestinal inflammation. In our preliminary study, liriodendrin inhibited NF-kB activation in sepsis-induced acute lung injury. The present study was designed to investigate its effect on dextran sulfate sodium (DSS)-induced colitis in a mouse model and to explore the possible related mechanisms. Experimental colitis was established by giving mice drinking water containing 3% (w/v) DSS for 7days...
September 20, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28941415/manganese-in-teeth-and-neurobehavior-sex-specific-windows-of-susceptibility
#3
Julia Anglen Bauer, Birgit Claus Henn, Christine Austin, Silvia Zoni, Chiara Fedrighi, Giuseppa Cagna, Donatella Placidi, Roberta F White, Qiong Yang, Brent A Coull, Donald Smith, Roberto G Lucchini, Robert O Wright, Manish Arora
BACKGROUND: Manganese (Mn) is an essential element required for growth and development, but higher body burdens have been associated with neurobehavioral decrements in children. OBJECTIVES: We examined whether prenatal or postnatal Mn measured in deciduous teeth was associated with scores on a test of visuospatial learning and memory. METHODS: Deciduous teeth were collected from 142 participants (ages 10-14years) residing near varied ferro‑manganese industry in Italy...
September 20, 2017: Environment International
https://www.readbyqxmd.com/read/28941414/synthesis-spectral-and-luminescence-study-crystal-structure-determination-and-dft-calculation-of-binuclear-palladium-ii-complexes
#4
S Seyfi, R Alizadeh, M Darvish Ganji, V Amani
Binuclear palladium(II) complexes with metal-metal (d(8)-d(8)) bonding interaction were synthesized by reactions of the 1-methyl-1H-1,2,3,4-tetrazole-5-thiol (Hmtzt) or a mixture of Hmtzt and 1,3-propanediamine (1,3-pda) ligands. Complex [Pd2(μ-mtzt)4]·2CH3CN (1) was synthesized by the reaction of Pd(OAc)2 with Hmtzt dissolved in acetonitrile and complex [Pd2(μ-mtzt)2(mtzt)2(1,3-pda)] (2) was synthesized by reaction of a mixture of Hmtzt and 1,3-propanediamine (dissolved in methanol) with PdCl2 (dissolved in acetonitrile) and were identified through elemental analysis, IR, UV-Vis, (1)H NMR, luminescence spectroscopy as well as single-crystal X-ray diffraction method...
September 14, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28941413/surface-analysis-of-2-mercapto-1-methylimidazole-adsorbed-on-copper-by-x-ray-photoelectron-spectroscopy
#5
Matjaž Finšgar
A detailed analysis using X-ray photoelectron spectroscopy (XPS) is presented for the system of 2-mercapto-1-methylimidazole (MMeI) adsorbed on Cu in 3wt% NaCl solution. High-resolution and survey XPS spectra and XPS-excited Auger L3M4,5M4,5 spectra were analysed in detail. Surface analysis revealed that the MMeI molecules do not lie flat on the surface via π-d interactions, but adsorb on the surface through an N-S-bridge configuration. Moreover, the characteristic Cu(I)-MMeI connection fingerprint, which is usually observed for these kinds of molecules, was not observed...
September 19, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28941412/rapid-determination-of-crocins-in-saffron-by-near-infrared-spectroscopy-combined-with-chemometric-techniques
#6
REVIEW
Shuailing Li, Qingsong Shao, Zhonghua Lu, Chengli Duan, Haojun Yi, Liyang Su
Saffron is an expensive spice. Its primary effective constituents are crocin I and II, and the contents of these compounds directly affect the quality and commercial value of saffron. In this study, near-infrared spectroscopy was combined with chemometric techniques for the determination of crocin I and II in saffron. Partial least squares regression models were built for the quantification of crocin I and II. By comparing different spectral ranges and spectral pretreatment methods (no pretreatment, vector normalization, subtract a straight line, multiplicative scatter correction, minimum-maximum normalization, eliminate the constant offset, first derivative, and second derivative), optimum models were developed...
September 13, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28941411/a-fully-continuous-system-of-dna-profile-evidence-evaluation-that-can-utilise-str-profile-data-produced-under-different-conditions-within-a-single-analysis
#7
Duncan Taylor, Jo-Anne Bright, Hannah Kelly, Meng-Han Lin, John Buckleton
The introduction of probabilistic DNA interpretation systems has made it possible to evaluate many profiles that previously (under a manual interpretation system) were not. These probabilistic systems have been around for a number of years and it is becoming more common that their use within a laboratory has spanned at least one technology change. This may be a change in laboratory hardware, the DNA profiling kit used, or the manner in which the profile is generated. Up until this point, when replicates DNA profiles are generated, that span a technological change, the ability to utilise all the information in all replicates has been limited or non-existent...
September 8, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28941410/the-contribution-of-human-non-human-animal-chimeras-to-stem-cell-research
#8
REVIEW
Sonya Levine, Laura Grabel
Chimeric animals are made up of cells from two separate zygotes. Human/non-human animal chimeras have been used for a number of research purposes, including human disease modeling. Pluripotent stem cell (PSC) research has relied upon the chimera approach to examine the developmental potential of stem cells, to determine the efficacy of cell replacement therapies, and to establish a means of producing human organs. Based on ethical issues, this work has faced pushback from various sources including funding agencies...
September 14, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28941409/a-comparison-of-steroid-profiles-in-the-testis-and-seminal-vesicle-of-the-catfish-heteropneustes-fossilis
#9
Radha Chaube, Surabhi Mishra, Rahul Kumar Singh
In the present study, distribution of steroid hormones (estradiol-17β (E2), testosterone (T), corticosteroids and progestins) in the testis and seminal vesicles (SV) of the catfish Heteropneustes fossilis were investigated in two seasons (pre-spawning and spawning phases) of the reproductive cycle. The data showed that the levels of the steroid hormones varied significantly in both reproductive phases and exhibited organ-related differences. Sex steroid levels were highest in the pre-spawning phase and lowest in the spawning phase...
September 9, 2017: Theriogenology
https://www.readbyqxmd.com/read/28941408/boar-seminal-plasma-inhibits-cryo-capacitation-of-frozen-thawed-ram-sperm-and-improves-fertility-following-intracervical-insemination
#10
Yi Fang, Rongzhen Zhong, Xiaosheng Zhang, Jinlong Zhang, Daowei Zhou
Cryopreservation has numerous deleterious effects on sperm structure and function, which can be reduced by adding seminal plasma (SP), either autologous or heterologous. The objective was to determine effects of adding boar SP to the freezing extender on sperm quality, tyrosine phosphorylation and fertilizing ability of frozen-thawed ram sperm. Semen was collected from eight Small-tail Han rams and extended in a glucose-egg yolk buffer supplemented with 0, 20, 40, or 60% porcine SP (from Large white boars)...
September 18, 2017: Theriogenology
https://www.readbyqxmd.com/read/28941407/targeted-neurogenesis-pathway-based-gene-analysis-identifies-adora2a-associated-with-hippocampal-volume-in-mild-cognitive-impairment-and-alzheimer-s-disease
#11
Emrin Horgusluoglu-Moloch, Kwangsik Nho, Shannon L Risacher, Sungeun Kim, Tatiana Foroud, Leslie M Shaw, John Q Trojanowski, Paul S Aisen, Ronald C Petersen, Clifford R Jack, Simon Lovestone, Andrew Simmons, Michael W Weiner, Andrew J Saykin
Alzheimer's disease (AD) patients display hippocampal atrophy, memory impairment, and cognitive decline. New neurons are generated throughout adulthood in 2 regions of the brain implicated in AD, the dentate gyrus of the hippocampus and the subventricular zone of the olfactory bulb. Disruption of this process contributes to neurodegenerative diseases including AD, and many of the molecular players in AD are also modulators of adult neurogenesis. However, the genetic mechanisms underlying adult neurogenesis in AD have been underexplored...
August 18, 2017: Neurobiology of Aging
https://www.readbyqxmd.com/read/28941406/theoretical-study-on-the-anomeric-effect-in-%C3%AE-substituted-tetrahydropyrans-and-piperidines
#12
Francisco A Martins, Josué M Silla, Matheus P Freitas
Conformational effects, including some controversial examples, have been reported in this work for 2-substituted tetrahydropyrans and piperidines, and for the respective protonated compounds [substituent = F, OH, OCH3, NH2, NHCH3 and N(CH3)2]. Hyperconjugation plays a major role in most cases, either due to endo or exo-anomeric interactions, especially when nitrogen is the electron donor to an antiperiplanar σ* orbital. This interaction also seems to contribute for the Perlin and reverse fluorine Perlin-like effects, which are related to the relative magnitude of (1)JC,H and (1)JC,F coupling constants, respectively, in axial and equatorial conformers...
September 15, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28941405/the-effect-of-zoledronate-containing-primer-on-dentin-bonding-of-a-universal-adhesive
#13
Walter Zenobi, Victor Pinheiro Feitosa, Maria Elisa Martins Moura, Camillo D'arcangelo, Lidiany Karla de Azevedo Rodrigues, Salvatore Sauro
OBJECTIVES: To evaluate the bonding ability and nanoleakage of a universal adhesive applied to dentin pre-treated using a zoledronate-containing primer (zol-primer) before and after mechanical load cycling. MATERIALS AND METHODS: Flat dentin surfaces obtained from human molars were assigned to one of the following adhesion procedures (n=6): 1-Single Bond Universal (SBU) applied in etch-and-rinse mode; 2- SBU applied as etch-and-rinse after the application of zol-primer; 3- SBU applied in self-etch strategy; 4- SBU applied as self-etch after the use of zol-primer...
September 14, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28941404/development-of-a-simple-multi-residue-determination-method-of-80-veterinary-drugs-in-oplegnathus-punctatus-by-liquid-chromatography-coupled-to-quadrupole-orbitrap-mass-spectrometry
#14
Fei Zhao, Xin Gao, Zhixu Tang, Xin Luo, Miaomiao Wu, Jiachao Xu, Xiaoting Fu
A simple, rapid and sensitive multi-residue analytical method was developed and validated for 80 veterinary drugs in Oplegnathus punctatus using ultrahigh performance liquid chromatography-Orbitrap high resolution mass spectrometry (LC-HRMS). The analytes belong to 12 different families include benzimidazoles, β-lactams, lincosamides, macrolides, nitromidazoles, quinolones, sulfonamides and trimethoprim, tetracyclines, triphenylmethane dyes, amphenicols, nonsteroidal estrogens and steroid hormones. The sample preparation was optimized base on QuEChERS (quick, easy, cheap, effective, rugged and safe) procedure...
September 18, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28941403/dual-function-of-a-cis-acting-rna-element-that-acts-as-a-replication-enhancer-and-a-translation-repressor-in-a-plant-positive-stranded-rna-virus
#15
Kiwamu Hyodo, Hikari Nagai, Tetsuro Okuno
The genome of red clover necrotic mosaic virus is divided into two positive-stranded RNA molecules of RNA1 and RNA2, which have no 5' cap structure and no 3' poly(A) tail. Previously, we showed that any mutations in the cis-acting RNA replication elements of RNA2 abolished its cap-independent translational activity, suggesting a strong link between RNA replication and translation. Here, we investigated the functions of the 5' untranslated region (UTR) of RNA2 and revealed that the basal stem-structure (5'BS) predicted in the 5' UTR is essential for robust RNA replication...
September 20, 2017: Virology
https://www.readbyqxmd.com/read/28941402/copper-ii-ions-interactions-in-the-systems-with-triamines-and-atp-potentiometric-and-spectroscopic-studies
#16
S K Hoffmann, J Goslar, R Bregier-Jarzebowska, A Gasowska, A Zalewska, L Lomozik
The mode of interaction and thermodynamic stability of complexes formed in binary and ternary Cu(II)/ATP/triamines systems were studied using potentiometric and spectroscopic (NMR, EPR, UV-Vis) methods. It was found that in binary metal-free systems ATP/HxPA species are formed (PA: Spd=spermidine or 3,3-tri=1,7-diamino-4-azaheptane) where the phosphate groups from nucleotides are preferred negative centers and protonated amine groups of amines are positive centers of reaction. In the ternary systems Cu/ATP/Hx(PA) as well as Cu/(ATP)(PA) species are formed...
September 9, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28941401/effect-based-assessment-of-toxicity-removal-during-wastewater-treatment
#17
Pia Välitalo, Riccardo Massei, Ilse Heiskanen, Peter Behnisch, Werner Brack, Andrew J Tindall, David Du Pasquier, Eberhard Küster, Anna Mikola, Tobias Schulze, Markus Sillanpää
Wastewaters contain complex mixtures of chemicals, which can cause adverse toxic effects in the receiving environment. In the present study, the toxicity removal during wastewater treatment at seven municipal wastewater treatment plants (WWTPs) was investigated using an effect-based approach. A battery of eight bioassays was applied comprising of cytotoxicity, genotoxicity, endocrine disruption and fish embryo toxicity assays. Human cell-based CALUX assays, transgenic larval models and the fish embryo toxicity test were particularly sensitive to WWTP effluents...
September 7, 2017: Water Research
https://www.readbyqxmd.com/read/28941400/coagulation-of-surface-water-observations-on-the-significance-of-biopolymers
#18
Zhaoyang Su, Ting Liu, Wenzheng Yu, Xing Li, Nigel J D Graham
Although the treatment of natural surface waters by coagulation has been investigated extensively, the detailed interaction between natural organic matter and alum is still not adequately understood or predictable, owing to the complexity of different components of the organic matrix and the conditions during coagulation. In this paper, we present the results of a novel approach to the study of the topic, which involved the progressive separation of organic components according to size, followed by coagulation of the filtrate solution, in order to expose the influence of particular organic fractions...
September 13, 2017: Water Research
https://www.readbyqxmd.com/read/28941399/comparison-of-uv-led-and-low-pressure-uv-for-water-disinfection-photoreactivation-and-dark-repair-of-escherichia-coli
#19
Guo-Qiang Li, Wen-Long Wang, Zheng-Yang Huo, Yun Lu, Hong-Ying Hu
Studies on ultraviolet light-emitting diode (UV-LED) water disinfection have shown advantages, such as safety, flexible design, and lower starting voltages. However, information about reactivation after UV-LED disinfection is limited, which is an important issue of UV light-based technology. In this study, the photoreactivation and dark repair of Escherichia coli after UV-LEDs and low pressure (LP) UV disinfection were compared. Four UV-LED units, 265 nm, 280 nm, the combination of 265 + 280 (50%), and 265 + 280 (75%) were tested...
September 17, 2017: Water Research
https://www.readbyqxmd.com/read/28941398/the-mechanisms-mediating-the-antiepileptic-effects-of-the-ketogenic-diet-and-potential-opportunities-for-improvement-with-metabolism-altering-drugs
#20
REVIEW
Neil A Youngson, Margaret J Morris, Bill Ballard
The ketogenic diet (KD) is increasingly being used to treat patients with intractable epilepsy. Despite decades of research, the reason for its success, when anticonvulsants have failed, is still not completely understood. There are, however, many candidate mechanisms which can be grouped into those that alter neuronal excitability at the synapse, and those that confer neuroprotection. The molecular underpinning of these mechanisms centres on the shift from glucose- to lipid-based energy generation that accompanies a high fat/low carbohydrate diet...
September 13, 2017: Seizure: the Journal of the British Epilepsy Association
keyword
keyword
2541
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"