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https://www.readbyqxmd.com/read/28922425/hypoxia-inducible-factor-1-alpha-as-a-therapeutic-target-for-primary-effusion-lymphoma
#1
Prabha Shrestha, David A Davis, Ravindra P Veeranna, Robert F Carey, Coralie Viollet, Robert Yarchoan
Primary effusion lymphoma (PEL) is an aggressive B-cell lymphoma with poor prognosis caused by Kaposi's sarcoma-associated herpesvirus (KSHV). Previous studies have revealed that HIF-1α, which mediates much of the cellular response to hypoxia, plays an important role in life cycle of KSHV. KSHV infection promotes HIF-1α activity, and several KSHV genes are in turn activated by HIF-1α. In this study, we investigated the effects of knocking down HIF-1α in PELs. We observed that HIF-1α knockdown in each of two PEL lines leads to a reduction in both aerobic and anaerobic glycolysis as well as lipid biogenesis, indicating that HIF-1α is necessary for maintaining a metabolic state optimal for growth of PEL...
September 18, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28921958/electronic-structural-analysis-of-copper-ii-tempo-abno-complexes-provides-evidence-for-copper-i-oxoammonium-character
#2
Richard C Walroth, Kelsey C Miles, James T Lukens, Samantha N MacMillan, Shannon S Stahl, Kyle M Lancaster
Copper/aminoxyl species are proposed as key intermediates in aerobic alcohol oxidation. Several possible electronic structural descriptions of these species are possible, and the present study probes this issue by examining four crystallographically characterized Cu/aminoxyl halide complexes by Cu K-edge, Cu L2,3-edge, and Cl K-edge X-ray absorption spectroscopy. The mixing coefficients between Cu, aminoxyl, and halide orbitals are determined via these techniques with support from density functional theory...
September 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28921743/exercise-training-dose-differentially-alters-muscle-and-heart-capillary-density-and-metabolic-functions-in-an-obese-rat-with-metabolic-syndrome
#3
Marcus Vinicius Machado, Aline Bomfim Vieira, Fabiana Gomes da Conceição, Alessandro Rodrigues Nascimento, Antonio Claudio Lucas da Nóbrega, Eduardo Tibirica
We evaluated the effect of frequency, duration and intensity of exercise training on metabolic parameters and structural capillary density in obese rats with metabolic syndrome (MS). Wistar-Kyoto rats were fed a either standard commercial diet (CON) or high-fat diet (HFD). Animals that received the HFD were randomly separated into either a sedentary (SED) group or 8 different exercise groups that varied by the frequency, duration and intensity of training. After 12 weeks of aerobic exercise training, body composition, aerobic capacity, hemodynamic variables, metabolic parameters and capillary density in the heart and skeletal muscle were evaluated...
September 17, 2017: Experimental Physiology
https://www.readbyqxmd.com/read/28921723/effect-of-prophylactic-cefalexin-treatment-on-the-development-of-bacterial-infection-in-acute-radiation-induced-dermatitis-in-dogs-a-blinded-randomized-controlled-prospective-clinical-trial
#4
Michele A Keyerleber, Lluís Ferrer
BACKGROUND: Acute radiation-induced dermatitis (ARID) is a common sequela of radiation therapy and carries the risk of secondary bacterial skin infection. No standard of care exists for managing canine ARID and evidence-based guidelines are lacking; however, prophylactic use of antibiotics is common. HYPOTHESIS/OBJECTIVES: To evaluate the impact of prophylactic cefalexin on the prevalence and severity of bacterial infection in canine ARID. ANIMALS: Seventeen dogs treated with definitive-intent radiotherapy...
September 18, 2017: Veterinary Dermatology
https://www.readbyqxmd.com/read/28920851/loktanella-acticola-sp-nov-isolated-from-seawater
#5
Sooyeon Park, Su Jung Choi, Sung-Min Won, Jung-Hoon Yoon
A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated OISW-6T, was isolated from seawater near Oido, a South Korean island, and subjected to a polyphasic taxonomic study. Strain OISW-6T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain OISW-6T fell within the clade comprising the type strains of Loktanella species. Strain OISW-6T exhibited 16S rRNA gene sequence similarity values of 97...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920850/ramlibacter-alkalitolerans-sp-nov-alkali-tolerant-bacterium-isolated-from-soil-of-ginseng
#6
Do-Hoon Lee, Chang-Jun Cha
A novel bacterial strain, designated CJ661T, was isolated from soil of ginseng in Anseong, South Korea. Cells of strain CJ661T were white-coloured, Gram-staining-negative, non-motile, aerobic and rod-shaped. Strain CJ661T grew optimally at 30 °C and pH 7.0. The analysis of 16S rRNA gene sequence of strain CJ661T showed that it belongs to the genus Ramlibacter within the family Comamonadaceae and was most closely related to Ramlibacter ginsenosidimutans KCTC 22276T (98.1 %), followed by Ramlibacter henchirensis DSM 14656T (97...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920849/flavimarina-flava-sp-nov-isolated-from-salicornia-herbacea
#7
Eui-Sang Cho, In-Tae Cha, Jung-Min Park, Hak-Jong Choi, Jong Hun Lee, Seong Woon Roh, Eun-Ah Cho, Mee-Hyang Kweon, Young-Do Nam, Myung-Ji Seo
A Gram-stain-negative, motile-by-gliding, aerobic, non-spore-forming, rod-shaped and yellow-pigmented bacterium was isolated from Salicornia herbacea in the Yellow Sea and designated as strain MBLN091T. It belonged to the family Flavobacteriaceae. The 16S rRNA gene sequence of this isolated strain was similar to that of Flavimarina pacifica IDSW-73T with 94.8 % similarity, and with 92.3-92.8 % similarities to those of other closely related species of the genus Leeuwenhoekiella. The similarities of the RNA polymerase subunit B gene between this strain and F...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920847/spirosoma-carri-sp-nov-isolated-from-an-automobile-air-conditioning-system
#8
Dong-Uk Kim, Hyosun Lee, Suyeon Lee, Sooyeon Park, Jung-Hoon Yoon, So Yoon Park, Jong-Ok Ka
A Gram-stain-negative and yellow-pigmented bacterial strain, designated TX0406T, was isolated from an automobile evaporator core collected in Korea. The cells were non-motile, aerobic and rod-shaped. The strain grew at 15-37 °C (optimum, 25 °C), at pH 6.0-7.0 (optimum, 6.5) and in the presence of 0-1.5 % (w/v) NaCl. Phylogenetically, the strain was related to members of the genus Spirosoma(93.7-90.7 % 16S rRNA sequence similarities) and showed the highest sequence similarity of 93.7 % to Spirosomapulveris JSH5-14T...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920841/desertibacillus-haloalkaliphilus-gen-nov-sp-nov-isolated-from-a-saline-desert
#9
Hitarth B Bhatt, M Azmatunnisa Begum, Sasikala Chintalapati, Venkata Ramana Chintalapati, Satya P Singh
Two Gram-stain-positive, rod-shaped and endospore-forming bacteria that represent a single species, designated strains KJ1-10-99T and KJ1-10-93, were isolated from a saline desert of Little Rann of Kutch, Gujarat, India. Analysis of 16S rRNA gene sequences revealed that the isolates belonged to the family Bacillaceae and were closely related to each other with 16S rRNA gene sequence similarity of 99.9 %. However, these two isolates formed a novel phylogenetic branch within this family. Both strains were aerobic, catalase and oxidase positive, and could grow optimally at 37 °C and pH 9...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920840/flavobacterium-dispersum-sp-nov-isolated-from-a-freshwater-spring
#10
Wen-Ming Chen, Ching-Ling Su, Shih-Yi Sheu
A novel bacterial strain MVW-23T was isolated from a freshwater spring in Taiwan. The strain was Gram-staining-negative, strictly aerobic, motile by gliding, rod-shaped and formed translucent yellow colonies. Optimal growth occurred at 20-30 °C, pH 7.0, and in the presence of 0.5-1 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MVW-23T belonged to the genus Flavobacterium and showed the highest levels of sequence similarity with respect to Flavobacterium denitrificans ED5T (97...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920839/natronotalea-proteinilytica-gen-nov-sp-nov-and-longimonas-haloalkaliphila-sp-nov-extremely-haloalkaliphilic-members-of-the-phylum-rhodothermaeota-from-hypersaline-alkaline-lakes
#11
Dimitry Y Sorokin, Tatiana V Khijniak, Erwin A Galinski, Ilya V Kublanov
Two proteolytic bacterial strains, BSker2T and BSker3T, were enriched from sediments of hypersaline alkaline lakes in Kulunda Steppe (Altai, Russia) with chicken feathers as substrate, followed by pure culture isolation on hypersaline alkaline media with casein. The cells were non-motile, filamentous, flexible rods. The isolates were obligately aerobic heterotrophs utilizing proteins and peptides as growth substrates. Both were obligate alkaliphiles, but differed in their pH optimum for growth: pH 9.5-9.8 for Bsker2T and pH 8...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920838/edaphobaculum-flavum-gen-nov-sp-nov-a-member-of-family-chitinophagaceae-isolated-from-grassland-soil
#12
Min Cao, Jing Huang, Jingxin Li, Zixu Qiao, Gejiao Wang
A yellow-coloured, Gram-stain-negative, non-motile, rod-shaped, strictly aerobic bacterium, designated 1-116T, was isolated from Erdos grassland soil of Inner Mongolia, PR China. Phylogenetic analysis based on 16S rRNA genes showed that strain 1-116T was a member of family Chitinophagaceae and exhibited the highest similarities to Taibaiella koreensis THG-DT86T (90.1 %) and Flavihumibacter solisivae 3-3T (90.0 %), while the similarities to the other Chitinophagaceae type strains were lower than 90.0 %...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920836/mycobacterium-aquiterrae-sp-nov-a-rapidly-growing-bacterium-isolated-from-groundwater
#13
Jae-Chan Lee, Kyung-Sook Whang
A strain representing a rapidly growing, Gram-stain-positive, aerobic, rod-shaped, non-motile, non-sporulating and non-pigmented species of the genus Mycobacterium, designated strain S-I-6T, was isolated from groundwater at Daejeon in Korea. The strain grew at temperatures between 10 and 37 °C (optimal growth at 25 °C), between pH 4.0 and 9.0 (optimal growth at pH 7.0) and at salinities of 0-5 % (w/v) NaCl, growing optimally with 2 % (w/v) NaCl. Phylogenetic analyses based on multilocus sequence analysis of the 16S rRNAgene, hsp65, rpoB and the 16S-23S internal transcribed spacer indicated that strain S-I-6T belonged to the rapidly growing mycobacteria, being most closely related to Mycobacterium sphagni...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920833/longispora-urticae-sp-nov-isolated-from-rhizosphere-soil-of-urtica-urens-l-and-emended-descriptions-of-the-species-longispora-albida-and-longisporafulva
#14
Chenyu Piao, Liying Jin, Junwei Zhao, Chongxi Liu, Yue Zhao, Xiangjing Wang, Wensheng Xiang
Two Gram-stain-positive, aerobic actinomycete strains, designated NEAU-PCY-3T and NEAU-PCY-4, were isolated from rhizosphere soil of Urtica urens L. collected from Anshan, Liaoning Province, northeast PR China. The 16S rRNA gene sequence analysis showed that the two strains exhibited 99.9 % 16S rRNA gene sequence similarity with each other and that they were most closely to Longispora fulva DSM 45356T (98.7, 98.9 %) and Longispora albida JCM 11711T (97.1, 97.2 %). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the two strains were located in the same lineage and formed a cluster with the genus Longispora...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920832/salinifilum-gen-nov-with-description-of-salinifilum-proteinilyticum-sp-nov-an-extremely-halophilic-actinomycete-isolated-from-meighan-wetland-iran-and-reclassification-of-saccharopolyspora-aidingensis-as-salinifilum-aidingensis-comb-nov-and-saccharopolyspora
#15
Mahdi Moshtaghi Nikou, Mohaddaseh Ramezani, Sharareh Harirchi, Somayyeh Makzoom, Mohammad Ali Amoozegar, Seyed Abolhassan Shahzadeh Fazeli, Peter Schumann, Antonio Ventosa
A Gram-positive, halophilic actinobacterial strain Miq-12T was isolated from Meighan wetland in Iran. Strain Miq-12T was strictly aerobic, catalase positive and oxidase negative. The isolate grew at 12-25 % NaCl, at 30-50 °C and pH 5.5-10.5. The optimum NaCl, temperature and pH for growth were 15-20 %, 40 °C and 7.0-8.0, respectively. The cell wall of strain Miq-12T contained meso-diaminopimelic acid as diagnostic diamino acid and arabinose as whole-cell sugar. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920831/algoriphagus-marisflavi-sp-nov-isolated-from-water-of-an-estuary-environment
#16
Sooyeon Park, Ji-Min Park, Jung-Hoon Yoon
A Gram-stain-negative, non-motile, aerobic and rod-shaped or ovoid bacterial strain, designated KEM-106T, was isolated from water of an estuary environment on the Yellow Sea, South Korea, and subjected to a polyphasic taxonomic study. Strain KEM-106T grew optimally at 30 °C and pH 7.0-8.0 and in the presence of 1.0-2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain KEM-106T belonged to the genus Algoriphagus, clustering with the type strains of Algoriphagus litorisediminis and Algoriphagus aquaemixtae...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920828/paenibacillus-arcticus-sp-nov-isolated-from-arctic-soil
#17
In-Tae Cha, Eui-Sang Cho, Yesol Yoo, Yoon Ji Seok, Inhye Park, Hee Seon Lim, Jung-Min Park, Seong Woon Roh, Young-Do Nam, Hak-Jong Choi, Yoo Kyung Lee, Myung-Ji Seo
A Gram-positive, endospore-forming, strictly aerobic and rod-shaped bacterium, designated strain MME2_R6T, was isolated from Arctic soil, and it was identified by using a polyphasic taxonomic approach. This strain was psychrotolerant, growing at 4‒24 °C. 16S rRNA gene sequence analysis showed that strain MME2_R6T was closest to Paenibacillus swuensis DY6T, with 93.9 % similarity. However, in phylogenetic analysis based on the 16S rRNA gene sequence, strain MME2_R6T showed that it clustered with Paenibacillus contaminans CKOBP-6T and the sequencing similarity between the two species was 93...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920827/marmoricola-endophyticus-sp-nov-an-endophytic-actinobacterium-isolated-from-thespesia-populnea
#18
Zhong-Ke Jiang, Zhen Pan, Fei-Na Li, Xiao-Jun Li, Shao-Wei Liu, Li Tuo, Ming-Guo Jiang, Cheng-Hang Sun
A novel endophytic actinobacterium, designated strain 8BXZ-J1T, was isolated from surface-sterilized branches of Thespesia populnea collected from Beilun Estuary Mangrove Forest National Nature Reserve in Guangxi, China, and examined by a polyphasic approach to determine its taxonomic position. Cells of the isolate were Gram-stain-positive, aerobic, non-spore-forming, non-motile and short rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 8BXZ-J1T belonged to the genus Marmoricola, sharing highest similarity with Marmoricola solisilvae DSM 27140T (96...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920826/hydrogenophaga-soli-sp-nov-isolated-from-rice-field-soil
#19
Dahye Yang, Seho Cha, Jiwon Choi, Taegun Seo
A novel Gram-stain-negative bacterial strain, designated strain S10T, was isolated from soil collected in a rice field in Goyang, South Korea. Cells of strain S10T were strictly aerobic, motile and rod-shaped. Colonies were round, convex, smooth and white. The strain grew optimally at 37 °C, pH 7.0 and 0 % (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene sequence of strain S10T revealed that the bacterium belongs to the family Comamonadaceae and is related to members of the genus Hydrogenophaga, with Hydrogenophaga caeni EMB71T being its closest relative (97...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920779/ocular-findings-and-select-ophthalmic-diagnostic-tests-in-captive-american-white-pelicans-pelecanus-erythrorhynchos
#20
Matthew E Kinney, Aaron C Ericsson, Craig L Franklin, Rebecca E H Whiting, Jacqueline W Pearce
The aim of this study was to establish normal ophthalmic parameters for select diagnostic tests in American white pelicans (Pelecanuserythrorhynchos). Twenty-one zoo-housed American white pelicans were manually restrained for noninvasive ocular diagnostic testing and complete ophthalmic examination. Tear production quantification using the phenol red thread test (PRTT), fluorescein staining, and intraocular pressure (IOP) evaluation were performed. In addition, conjunctival aerobic bacterial culture and culture-independent 16S rRNA amplicon sequencing were performed on select eyes...
September 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
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