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https://www.readbyqxmd.com/read/28103156/antibodies-against-henipa-like-viruses-in-brazilian-bats
#1
Jansen de Araujo, Michael K Lo, Azaibi Tamin, Tatiana L Ometto, Luciano M Thomazelli, Marcello S Nardi, Renata F Hurtado, Alessandra Nava, Christina F Spiropoulou, Paul A Rota, Edison L Durigon
Bats are reservoir hosts for many paramyxoviruses, some of which cause human and zoonotic diseases of public health importance. We developed a Nipah virus nucleoprotein enzyme-linked immunosorbent assay to detect cross-reactive antibodies in serum samples from several bat species in Brazil. Our results warrant further investigation of henipa-like virus reservoirs in the Western hemisphere.
January 19, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28102432/synthesis-of-human-parainfluenza-virus-4-nucleocapsid-like-particles-in-yeast-and-their-use-for-detection-of-virus-specific-antibodies-in-human-serum
#2
Aistė Bulavaitė, Rita Lasickienė, Paulius Lukas Tamošiūnas, Martynas Simanavičius, Kęstutis Sasnauskas, Aurelija Žvirblienė
The aim of this study was to produce human parainfluenza virus type 4 (HPIV4) nucleocapsid (N) protein in yeast Saccharomyces cerevisiae expression system, to explore its structural and antigenic properties and to evaluate its applicability in serology. The use of an optimized gene encoding HPIV4 N protein amino acid (aa) sequence GenBank AGU90031.1 allowed high yield of recombinant N protein forming nucleocapsid-like particles (NLPs) in yeast. A substitution L332D disrupted self-assembly of NLPs, confirming the role of this position in the N proteins of Paramyxovirinae...
January 19, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28102392/dynamics-of-the-o-h2-%C3%A2-oh-h-oh-h-proton-and-hydrogen-atom-transfer-reactions-on-the-two-lowest-potential-energy-surfaces
#3
Rodrigo Martínez, Miguel Paniagua, Jordi Mayneris-Perxachs, Pablo Gamallo, Miguel González
The dynamics of the title reaction was studied using mainly the quasiclassical trajectory (QCT) method on the ground 1(2)A'' (OH(+) channel) and first excited 1(2)A' (OH channel) potential energy surfaces (PESs) employing ab initio analytical representations of the PESs developed by us. Both PESs correspond to exoergic reactions, are barrierless and present a deep minimum along the minimum energy path (MEP). Some extra calculations (cross sections) were also performed with the time dependent quantum real wave packet method at the centrifugal sudden level (RWP-CS method)...
January 19, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28101488/thyroid-function-and-high-sensitivity-c-reactive-protein-in-cross-sectional-results-from-the-brazilian-longitudinal-study-of-adult-health-elsa-brasil-effect-of-adiposity-and-insulin-resistance
#4
Érique José F Peixoto de Miranda, Márcio Sommer Bittencourt, Itamar S Santos, Paulo A Lotufo, Isabela M Benseñor
BACKGROUND: Subclinical hypothyroidism (SCH) is associated with an increased cardiovascular risk, but little information is available about its association with high-sensitivity C-reactive protein (hs-CRP). OBJECTIVES: This study aims to analyze the association between SCH and hs-CRP using baseline data from the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). METHODS: The study has a cross-sectional design. We included subjects with normal thyroid function (thyroid-stimulating hormone, TSH, 0...
December 2016: European Thyroid Journal
https://www.readbyqxmd.com/read/28100617/differences-in-glycoprotein-complex-gpc-receptor-binding-site-accessibility-prompt-poor-cross-reactivity-of-neutralizing-antibodies-between-closely-related-arenaviruses
#5
Rachel B Brouillette, Elisabeth K Phillips, Natarajan Ayithan, Wendy Maury
: The glycoprotein complex (GPC) of arenaviruses, composed of stable signal peptide (SSP), GP1, and GP2, is the only antigen correlated with antibody-mediated neutralization. However, despite strong cross-reactivity of convalescent antisera between related arenavirus species, weak or no cross-neutralization occurs. Two closely related Clade B viruses, Machupo virus (MACV) and Junín virus (JUNV), have near identical overall GPC architecture and share a host receptor, transferrin receptor 1...
January 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28100497/avian-derived-and-human-seasonal-derived-hemagglutinin-proteins-elicit-cd4-t-cell-responses-that-are-comparable-in-epitope-abundance-and-diversity
#6
Anthony DiPiazza, Katherine Richards, Nicholas Poulton, Andrea J Sant
Avian influenza viruses remain a significant concern due to their pandemic potential. Vaccine trials have suggested that humans respond poorly to avian influenza vaccines, relative to seasonal vaccines. It is important to understand, first, if there is a general deficiency in the ability of avian HA proteins to generate immune responses and if so, what underlies this defect. This question is of particular interest because it has been suggested that in humans, the poor immunogenicity of H7 vaccines may be due to a paucity of CD4 T cell epitopes...
January 18, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28098101/insulin-resistance-in-predialytic-nondiabetic-chronic-kidney-disease-patients-a-hospital-based-study-in-eastern-uttar-pradesh-india
#7
Neha Srivastava, R G Singh, Alok Kumar, Shivendra Singh
Most investigations have focused on patients with end-stage renal disease (ESRD). More recently, due to increased recognition of the high prevalence of moderate-to-severe chronic kidney disease (CKD), attention has been redirected to this patient population to identify risk factors associated with hospitalization, death, and progression to ESRD. The objective of this study was to examine the degree and determinants of insulin resistance (IR) in predialytic, nondiabetic, CKD patients. Our study is a hospital-based cross-sectional study...
January 2017: Saudi Journal of Kidney Diseases and Transplantation
https://www.readbyqxmd.com/read/28096437/phase-angle-predicts-arterial-stiffness-and-vascular-calcification-in-peritoneal-dialysis-patients
#8
Margarida Sarmento-Dias, Carla Santos-Araújo, Rui Poínhos, Bruno Oliveira, Maria Sousa, Liliana S Silva, Isabel S Silva, Flora Correia, Manuel Pestana
♦ Objectives: Fluid overload (FO) is frequently present in peritoneal dialysis (PD) patients and is associated with markers of malnutrition, inflammation, and atherosclerosis/calcification (MIAC) syndrome. We examined the relationships in stable PD patients between phase angle (PhA) and the spectrum of uremic vasculopathy including vascular calcification and arterial stiffness and between PhA and changes in serum fetuin-A levels. ♦ Methods: Sixty-one stable adult PD patients were evaluated in a cross-sectional study (ST1)...
January 17, 2017: Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis
https://www.readbyqxmd.com/read/28096058/anemia-at-the-time-of-diagnosis-of-inflammatory-bowel-disease-prevalence-and-associated-factors-in-adolescent-and-adult-patients
#9
Alfredo J Lucendo, Ángel Arias, Óscar Roncero, Daniel Hervías, Cristina Verdejo, Carmen Naveas-Polo, Abdelmouneim Bouhmidi, Rufo Lorente, Luis Miguel Alcázar, Irina Salueña, Julio A García-Quiñones, María Jesús Carrillo-Ramos
BACKGROUND: The prevalence, characteristic and determinants of anemia, at the time of inflammatory bowel disease (IBD) diagnosis have yet to be fully elucidated. METHODS: Retrospective cross-sectional study. Analytical data and disease characteristics obtained upon diagnosis of 1278 IBD patients [Crohn's disease/ulcerative colitis (CD/UC): 718/560] were collected. RESULTS: Anemia was present in 41.2% of patients at diagnosis (47% and 33.8% of CD and UC patients, respectively; p<0...
December 14, 2016: Digestive and Liver Disease
https://www.readbyqxmd.com/read/28095436/arraypitope-automated-analysis-of-amino-acid-substitutions-for-peptide-microarray-based-antibody-epitope-mapping
#10
Christian Skjødt Hansen, Thomas Østerbye, Paolo Marcatili, Ole Lund, Søren Buus, Morten Nielsen
Identification of epitopes targeted by antibodies (B cell epitopes) is of critical importance for the development of many diagnostic and therapeutic tools. For clinical usage, such epitopes must be extensively characterized in order to validate specificity and to document potential cross-reactivity. B cell epitopes are typically classified as either linear epitopes, i.e. short consecutive segments from the protein sequence or conformational epitopes adapted through native protein folding. Recent advances in high-density peptide microarrays enable high-throughput, high-resolution identification and characterization of linear B cell epitopes...
2017: PloS One
https://www.readbyqxmd.com/read/28094980/fe-catalyzed-c-c-bond-construction-from-olefins-via-radicals
#11
Julian C Lo, Dongyoung Kim, Chung-Mao Pan, Jacob T Edwards, Yuki Yabe, Jinghan Gui, Tian Qin, Sara Gutiérrez, Jessica Giacoboni, Myles W Smith, Patrick L Holland, Phil S Baran
This full report details the development of the iron-catalyzed conversion of olefins to radicals and their subsequent use in the construction of C-C bonds. Optimization of a reductive diene cyclization led to the development of an intermolecular cross-coupling of electronically differentiated donor and acceptor olefins. Although the substitution on the donor olefins was initially limited to alkyl and aryl groups, additional efforts culminated in the expansion of the scope of the substitution to various heteroatom-based functionality, providing a unified olefin reactivity...
January 17, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28094882/association-of-cardiorespiratory-fitness-and-adiposity-with-inflammatory-biomarkers-in-young-adults
#12
Eunduck Park, Janet C Meininger, Duck-Hee Kang, Kelley Pettee Gabriel, Nikhil S Padhye
OBJECTIVES: Cardiorespiratory fitness (fitness) has been inversely associated with inflammation, but whether the association is attributed to fitness itself or lower levels of adiposity remains uncertain in young adults. The purpose of this study was to determine the association of fitness and adiposity with inflammation in young adults. METHODS: A cross-sectional study was conducted with 88 participants aged 20-34 years. Fitness was assessed by a submaximal treadmill walking test...
January 17, 2017: American Journal of Human Biology: the Official Journal of the Human Biology Council
https://www.readbyqxmd.com/read/28094253/dynamical-importance-of-van-der-waals-saddle-and-excited-potential-surface-in-c-1-d-d2-complex-forming-reaction
#13
Zhitao Shen, Haitao Ma, Chunfang Zhang, Mingkai Fu, Yanan Wu, Wensheng Bian, Jianwei Cao
Encouraged by recent advances in revealing significant effects of van der Waals wells on reaction dynamics, many people assume that van der Waals wells are inevitable in chemical reactions. Here we find that the weak long-range forces cause van der Waals saddles in the prototypical C((1)D)+D2 complex-forming reaction that have very different dynamical effects from van der Waals wells at low collision energies. Accurate quantum dynamics calculations on our highly accurate ab initio potential energy surfaces with van der Waals saddles yield cross-sections in close agreement with crossed-beam experiments, whereas the same calculations on an earlier surface with van der Waals wells produce much smaller cross-sections at low energies...
January 17, 2017: Nature Communications
https://www.readbyqxmd.com/read/28094237/a-multiplex-microsphere-immunoassay-for-zika-virus-diagnosis
#14
Susan J Wong, Andrea Furuya, Jing Zou, Xuping Xie, Alan P Dupuis, Laura D Kramer, Pei-Yong Shi
Rapid and accurate diagnosis of infectious agents is essential for patient care, disease control, and countermeasure development. The present serologic diagnosis of Zika virus (ZIKV) infection relies mainly on IgM-capture ELISA which is confounded with the flaw of cross-reactivity among different flaviviruses. In this communication, we report a multiplex microsphere immunoassay (MIA) that captures the diagnostic power of viral envelope protein (that elicits robust, yet cross-reactive antibodies to other flaviviruses) and the differential power of viral nonstructural proteins NS1 and NS5 (that induce more virus-type specific antibodies)...
January 10, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28093913/synergistic-photoredox-nickel-coupling-of-acyl-chlorides-with-secondary-alkyltrifluoroborates-dialkyl-ketone-synthesis
#15
Javad Amani, Gary A Molander
Visible light photoredox/nickel dual catalysis has been employed in the cross-coupling of acyl chlorides with potassium alkyltrifluoroborates. This protocol, based on single-electron-mediated alkyl transfer, circumvents the restriction of using reactive alkylmetallic nucleophiles in transition-metal-catalyzed acylation and achieves a mild and efficient method for the synthesis of unsymmetrical alkyl ketones. In this approach, a variety of acyl chlorides have been successfully coupled with structurally diverse potassium alkyltrifluoroborates, generating the corresponding ketones with good yields...
January 17, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28093849/obesity-and-type-2-diabetes-are-associated-with-elevated-pcsk9-levels-in-young-women
#16
Amy E Levenson, Amy S Shah, Philip R Khoury, Thomas R Kimball, Elaine M Urbina, Sarah D de Ferranti, David M Maahs, Lawrence M Dolan, R Paul Wadwa, Sudha B Biddinger
BACKGROUND: Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a key regulator of low-density lipoprotein cholesterol and cardiovascular disease risk, and is an emerging therapeutic target. OBJECTIVE: We compared serum PCSK9 levels in young adults, with and without type 2 diabetes. SUBJECTS AND METHODS: Cross-sectional analysis was conducted in a cohort, aged 15 to 26 years, in Cincinnati, OH, from 2005 to 2010. Serum PCSK9 levels were measured in 94 youth with type 2 diabetes, 93 obese control subjects, and 99 lean control subjects...
January 17, 2017: Pediatric Diabetes
https://www.readbyqxmd.com/read/28093528/a-b-cell-epitope-peptide-derived-from-the-major-grass-pollen-allergen-phl-p-1-boosts-allergen-specific-secondary-antibody-responses-without-allergen-specific-t-cell-help
#17
Meena Narayanan, Raphaela Freidl, Margarete Focke-Tejkl, Ulrike Baranyi, Thomas Wekerle, Rudolf Valenta, Birgit Linhart
More than 40% of allergic patients suffer from grass pollen allergy. Phl p 1, the major timothy grass pollen allergen, belongs to the cross-reactive group 1 grass pollen allergens that are thought to initiate allergic sensitization to grass pollen. Repeated allergen encounter boosts allergen-specific IgE production and enhances clinical sensitivity in patients. To investigate immunological mechanisms underlying the boosting of allergen-specific secondary IgE Ab responses and the allergen epitopes involved, a murine model for Phl p 1 was established...
January 16, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28092944/reaction-chemistry-to-characterize-the-molecular-initiating-event-in-skin-sensitization-a-journey-to-be-continued
#18
Andreas Natsch, Roger Emter
The publication by the OECD of the adverse outcome pathway (AOP) for skin sensitization has accelerated the development and validation of mechanistic tests and testing strategies to assess the potential of new molecules to trigger skin allergies. The molecular initiating event (MIE) in the AOP is reaction with skin peptides/proteins. It is followed by a number of cellular events. Currently, only one in chemico test to characterize the MIE was proposed to and underwent adoption by the OECD, while two cell-based assays have completed the process...
January 17, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28092084/iron-availability-compromises-not-only-oligodendrocytes-but-also-astrocytes-and-microglial-cells
#19
Maria Victoria Rosato-Siri, Leandro Marziali, María Eugenia Guitart, Maria Elvira Badaracco, Mariana Puntel, Fernando Pitossi, Jorge Correale, Juana Maria Pasquini
When disrupted, iron homeostasis negatively impacts oligodendrocyte (OLG) differentiation and impairs myelination. To better understand myelin formation and OLG maturation, in vivo and in vitro studies were conducted to evaluate the effect of iron deficiency (ID) not only on OLG maturation but also on astrocytes (AST) and microglial cells (MG). In vivo experiments in an ID model were carried out to describe maturational events during OLG and AST development and the reactive profile of MG during myelination when iron availability is lower than normal...
January 14, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28092067/interrelations-among-the-adipocytokines-leptin-and-adiponectin-oxidative-stress-and-aseptic-inflammation-markers-in-pre-and-early-pubertal-normal-weight-and-obese-boys
#20
George Paltoglou, Maria Schoina, George Valsamakis, Nicolaos Salakos, Alexandra Avloniti, Athanasios Chatzinikolaou, Alexandra Margeli, Chrysanthi Skevaki, Maria Papagianni, Christina Kanaka-Gantenbein, Ioannis Papassotiriou, George P Chrousos, Ioannis G Fatouros, George Mastorakos
PURPOSE: Presumed interrelationships among deleterious aspects of adipose tissue metabolism, inflammation, and cellular oxidative stress could be influenced by pubertal hormonal changes. They were investigated in pre- and early pubertal normal-weight and obese boys before and after an exercise bout employed as an energy demanding stimulator. METHODS: Cross-sectional study. Seventy-six healthy pre- (mean ± SD, 10.6 ± 0.2 years old, 28 normal-weight, and 11 obese) and early-(11...
January 16, 2017: Endocrine
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