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https://www.readbyqxmd.com/read/29156505/cross-reactivity-of-polyclonal-antibodies-against-canavalia-ensiformis-jack-bean-urease-and-h-pylori-urease-subunit-a-fragments
#1
Zbigniew Jerzy Kaminski, Inga Relich, Iwona Konieczna, Wieslaw Kaca, Beata Kolesinska
Overlapping decapeptide fragments of H. pylori urease subunit A (UreA) were synthesized and tested with polyclonal antibodies against Canavalia ensiformis (Jack bean) urease. The linear epitopes of UreA identified using the dot blot method were then examined using epitope mapping. For this purpose, series of overlapping fragments of UreA, frameshifted +/- 4 amino acid residues were synthesized. Most of the UreA epitopes which reacted with the Jack bean urease polyclonal antibodies had been recognized in previous studies by monoclonal antibodies against H...
November 20, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/29156363/quality-of-life-is-associated-with-chronic-inflammation-in-depression-a-cross-sectional-study
#2
M Faugere, J-A Micoulaud-Franchi, C Faget-Agius, C Lançon, M Cermolacce, R Richieri
BACKGROUND: Inflammation may play a crucial role in the pathophysiology of depression. However, the association between chronic inflammation and health outcomes in depression remains unclear, particularly for patient-reported outcomes. METHODS: The aim of this study was to investigate the relationship between quality of life (QoL) (physical and mental health, assessed by the SF-36) and chronic inflammation assessed using C-reactive protein (CRP) in patients with current major depressive disorder...
November 14, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/29156361/non-suicidal-self-injury-in-referred-adolescents-with-mood-disorders-and-its-association-with-cyclothymic-hypersensitive-temperament
#3
Gabriele Masi, Annarita Milone, Anna Rita Montesanto, Elena Valente, Simone Pisano
BACKGROUND: Non suicidal self-injuries (NSSIs) are deliberate self-harm behaviors without suicidal intent, usually starting in adolescence, with increasing rates of occurrence both in epidemiological and clinical samples. Several studies associated cyclothymic-hypersensitive temperament (CHT) with self-harm behaviors and suicidal risk. Aim of this study is to explore the association between NSSIs and CHT in a clinical sample of adolescents. We hypothesized that CHT may differentiate NSSI from non-NSSI adolescents with mood disorders, when other psychopathological features are controlled for...
November 13, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/29156294/phospholipase-a1-based-cross-reactivity-among-venoms-of-clinically-relevant-hymenoptera-from-neotropical-and-temperate-regions
#4
Amilcar Perez-Riverol, Luís Gustavo Romani Fernandes, Alexis Musacchio Lasa, José Roberto Aparecido Dos Santos-Pinto, Débora Moitinho Abram, Gabriel Hideki Izuka Moraes, Frederic Jabs, Michaela Miehe, Henning Seismman, Mario Sergio Palma, Ricardo de Lima Zollner, Edzard Spillner, Márcia Regina Brochetto-Braga
Molecular cross-reactivity caused by allergen homology or cross-reactive carbohydrate determinants (CCDs) is a major challenge for diagnosis and immunotherapy of insect venom allergy. Venom phospholipases A1 (PLA1s) are classical, mostly non-glycosylated wasp and ant allergens that provide diagnostic benefit for differentiation of genuine sensitizations from cross-reactivity. As CCD-free molecules, venom PLA1s are not causative for CCD-based cross-reactivity. Little is known however about the protein-based cross-reactivity of PLA1 within vespid species...
November 17, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29155743/snap-chip-for-cross-reactivity-free-and-spotter-free-multiplexed-sandwich-immunoassays
#5
Huiyan Li, Sebastien Bergeron, Heidi Larkin, David Juncker
Multiplexed protein analysis has shown superior diagnostic sensitivity and accuracy compared to single proteins. Antibody microarrays allow for thousands of micro-scale immunoassays performed simultaneously on a single chip. Sandwich assay format improves assay specificity by detecting each target with two antibodies, but suffers from cross-reactivity between reagents thus limiting their multiplexing capabilities. Antibody colocalization microarray (ACM) has been developed for cross-reactivity-free multiplexed protein detection, but requires an expensive spotter on-site for microarray fabrication during assays...
November 13, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155436/enzyme-replacement-therapy-for-infantile-onset-pompe-disease
#6
REVIEW
Min Chen, Lingli Zhang, Shuyan Quan
BACKGROUND: Infantile-onset Pompe disease is a rare and progressive autosomal-recessive disorder caused by a deficiency of the lysosomal enzyme acid alpha-glucosidase (GAA). Current treatment involves enzyme replacement therapy (with recombinant human alglucosidase alfa) and symptomatic therapies (e.g. to control secretions). Children who are cross-reactive immunological material (CRIM)-negative require immunomodulation prior to commencing enzyme replacement therapy.Enzyme replacement therapy was developed as the most promising therapeutic approach for Pompe disease; however, the evidence is lacking, especially regarding the optimal dose and dose frequency...
November 20, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29154883/site-specific-labeling-of-two-proteins-in-one-system-by-atypical-split-inteins
#7
Xue Li, Xiao-Ling Zhang, Yu-Ming Cai, Lu Zhang, Ying Lin, Qing Meng
Atypical inteins have been used for protein site-specific labeling and modification. S1 or S11 intein contain a small N-intein or C-intein which can be chemically synthesized and added with desired labels or modifications. However, seldom reports have been found to develop multi-protein specific labeling in one system at the same time. Here, we established a specific labeling method for two proteins based on three pairs of atypical inteins, RBS1 (Rma DnaB S1)/SGS1 (Ssp GyrB S1) intein, TE3S11 (Ter DnaE-3 S11)/SGS11 (Ssp GyrB S11) intein, and RBS1/TE3S11 intein...
November 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29154455/translational-evidence-of-prothrombotic-and-inflammatory-endothelial-damage-in-cushing-syndrome-after-remission
#8
Gloria Aranda, Rebeca Fernandez-Ruiz, Marta Palomo, Mónica Romo, Mireia Mora, Irene Halperin, Gregori Casals, Joaquim Enseñat, Oscar Vidal, Maribel Diaz-Ricart, Felicia A Hanzu
OBJECTIVE: Sustained evidence from observational studies indicates that after remission of Cushing Syndrome (CS) a cardiovascular risk phenotype persists. Here we performed a translational study in active CS and CS in remission to evaluate the subclinical cardiometabolic burden and to explore the direct proinflammatory and prothrombotic potential of their sera on the endothelium in an in vitro translational atherothrombotic cell model. PATIENTS: Cross-sectional study...
November 20, 2017: Clinical Endocrinology
https://www.readbyqxmd.com/read/29152874/concordance-and-correlation-of-activity-indices-in-patients-with-rheumatoid-arthritis-in-northwestern-colombia-a-cross-sectional-study
#9
Ricardo Restrepo-Correa, Libia M Rodríguez-Padilla, Aura L Zapata-Castellanos, Andrea Ocampo, Juan J García, Carolina Muñoz-Grajales, Luis F Pinto-Peñaranda, Javier D Márquez-Hernández, Miguel A Mesa-Navas, Carlos J Velásquez-Franco
AIM: To determine the correlation and concordance between different clinimetric scores in patients with rheumatoid arthritis in two high-complexity reference centers in northwestern Colombia. METHOD: A cross-sectional study in adults diagnosed with rheumatoid arthritis was conducted according to the 2010 American College of Rheumatology and European League Against Rheumatism Classification Criteria, between January and June, 2013. The correlation was evaluated using Spearman's correlation coefficient, and concordance with quadratic weighted kappa with the respective confidence intervals, for which patients were classified into different categories of disease activity...
November 19, 2017: International Journal of Rheumatic Diseases
https://www.readbyqxmd.com/read/29152733/factorial-and-convergent-validity-of-the-youth-psychopathic-traits-inventory-short-version-in-ghana
#10
Samuel Adjorlolo, Bruce D Watt
Studies have shown the existence of psychopathy construct in Western cultures. However, there is a general lack of research on this topical issue in non-Western countries, specifically sub-Saharan Africa. Consequently, this study investigated the factorial and convergent validity of the Youth Psychopathic Traits Inventory-Short Version (YPI-S) in 327 adolescents in Ghana. Confirmatory factor analysis supported a three-factor structure (interpersonal, behavioural and affective) of psychopathy that was invariant across gender...
November 20, 2017: International Journal of Psychology: Journal International de Psychologie
https://www.readbyqxmd.com/read/29151819/production-of-monoclonal-antibodies-against-human-trefoil-factor-3-and-development-of-a-modified-sandwich-elisa-for-detection-of-trefoil-factor-3-homodimer-in-saliva
#11
Saichit Khummuang, Waraporn Phanphrom, Witida Laopajon, Watchara Kasinrerk, Ponlatham Chaiyarit, Supansa Pata
Background: Human trefoil factor (TFF) peptides consist of three members: TFF1, TFF2 and TFF3. TFF3 is the most abundant TFF peptide in saliva. TFF3 homodimer was suggested to be involved in apoptosis inhibition and malignancy. Determination of TFF3 homodimer expression profiles in saliva may lead to new information about oral biology and diseases. The objective of this study was to generate monoclonal antibodies (mAbs) against TFF3 and apply the produced mAbs for the establishment of ELISA for quantification of dimeric TFF3 in saliva...
2017: Biological Procedures Online
https://www.readbyqxmd.com/read/29151654/development-and-evaluation-of-simple-dot-blot-assays-for-rapid-detection-of-staphylococcal-enterotoxin-a-in-food
#12
Mamta Singh, Ravi Kant Agrawal, Bhoj Raj Singh, Sanjod Kumar Mendiratta, Rajesh Kumar Agarwal, Mithilesh Kumar Singh, Deepak Kumar
The present study was aimed to develop and evaluate dot-blot assays for rapid detection of staphylococcal enterotoxin-A (SEA) in food. Dot blots were developed in two formats, indirect and sandwich utilizing mouse monoclonal anti-SEA and rabbit polyclonal anti-SEA antibodies. In indirect dot-blot format, recombinant SEA was directly coated on NCM dot-blot strip and detection was carried out by anti-SEA antibodies. In sandwich dot-blot format, SEA was trapped between anti-SEA capture and detection antibodies...
December 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29151492/determinants-of-the-change-in-arterial-stiffness-in-peritoneal-dialysis-patients
#13
Mitsutaka Nakahigashi, Hiroyasu Tsukaguchi, Satoshi Morimoto, Chikara Nakano, Hiroko Ueda, Kazunori Someya, Makiko Kusabe, Sanae Kikuchi, Takanobu Imada, Ichiro Shiojima
Arterial stiffness is an important risk factor for cardiovascular disease (CVD) in patients with end-stage renal failure. However, little is known about the factors that contribute to arterial rigidity in peritoneal dialysis (PD) patients. The aim of this study was to define the pattern and determinants of the longitudinal change in arterial stiffness after PD initiation.Arterial stiffening was estimated for 46 PD patients by using brachial-ankle pulse wave velocity (baPWV) and carotid intima-media thickness (cIMT)...
November 17, 2017: International Heart Journal
https://www.readbyqxmd.com/read/29149089/mnsod-and-cyclin-b1-coordinate-a-mito-checkpoint-during-cell-cycle-response-to-oxidative-stress
#14
Amanda L Kalen, Iman M Ahmad, Maher Y Abdalla, Yunxia Q O'Malley, Prabhat C Goswami, Ehab H Sarsour
Communication between the nucleus and mitochondrion could coordinate many cellular processes. While the mechanisms regulating this communication are not completely understood, we hypothesize that cell cycle checkpoint proteins coordinate the cross-talk between nuclear and mitochondrial functions following oxidative stress. Human normal skin fibroblasts, representative of the G₂-phase, were irradiated with 6 Gy of ionizing radiation and assayed for cyclin B1 translocation, mitochondrial function, reactive oxygen species (ROS) levels, and cytotoxicity...
November 17, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/29148593/first-isolation-of-vibrio-tapetis-and-an-atypical-strain-of-aeromonas-salmonicida-from-skin-ulcerations-in-common-dab-limanda-limanda-in-the-north-sea
#15
M Vercauteren, E De Swaef, A Declercq, L Bosseler, S Gulla, S Balboa, J L Romalde, L Devriese, H Polet, F Boyen, K Chiers, A Decostere
Skin ulcerations rank amongst the most prevalent lesions affecting wild common dab (Limanda limanda) with an increase in prevalence of up to 3.5% in the Belgian part of the North Sea. A complex aetiology of these ulcerations is suspected, and many questions remain on the exact factors contributing to these lesions. To construct the aetiological spectrum of skin ulcerations in flatfish, a one-day monitoring campaign was undertaken in the North Sea. Fifteen fish presented with one or more ulcerations on the pigmented and/or non-pigmented side...
November 17, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/29148260/peri-implant-parameters-and-c-reactive-protein-levels-among-patients-with-different-obesity-levels
#16
Fahim Vohra, Fahad Alkhudhairy, Abdulaziz A Al-Kheraif, Zohaib Akram, Fawad Javed
BACKGROUND: It is hypothesized that peri-implant conditions are worse with increasing severity of obesity, because systemic low-grade inflammatory marker (C-reactive protein [CRP]) is higher in severe form of obese individuals. PURPOSE: The aim of the cross-sectional retrospective study was to compare clinical and radiographic peri-implant inflammatory parameters in patients with different levels of obesity and correlate these parameters with CRP levels. MATERIALS AND METHODS: Eighty-four patients who participated in this study were divided into 4 groups: class I obese (group 1), class II obese (group 2), class III obese (group 3), and nonobese individuals (group 4) were included...
November 16, 2017: Clinical Implant Dentistry and related Research
https://www.readbyqxmd.com/read/29147838/the-reactive-proactive-aggression-questionnaire-measurement-invariance-and-reliability-among-a-school-sample-of-portuguese-youths
#17
Pedro Pechorro, Lara Ayala-Nunes, Rachel Kahn, Cristina Nunes
Aggression is an important risk factor for various forms of problem behaviors in adolescence, and research has often distinguished between reactive and proactive forms of aggression. The aim of the present study was to compare the psychometric properties (i.e., structural invariance, internal consistency, and criterion validity) of the Reactive-Proactive Aggression Questionnaire (RPQ) between males and females among a mixed-gender school sample of 782 Portuguese youth (M = 15.87 years, SD = 1.72). Confirmatory factor analysis revealed that a two-factor first-order structure obtained the best fit and cross-gender measurement invariance was demonstrated after excluding item 21...
November 17, 2017: Child Psychiatry and Human Development
https://www.readbyqxmd.com/read/29147812/geissoschizine-synthase-controls-flux-in-the-formation-of-monoterpenoid-indole-alkaloids-in-a-catharanthus-roseus-mutant
#18
Yang Qu, Antje M K Thamm, Matthew Czerwinski, Sayaka Masada, Kyung Hee Kim, Graham Jones, Ping Liang, Vincenzo De Luca
A Catharanthus roseus mutant accumulates high levels of ajmalicine at the expense of catharanthine and vindoline. The altered chemistry depends on increased expression and biochemical activities of strictosidine β-glucosidase and ajmalicine synthase activities and reduced expression and biochemical activity of geissoschizine synthase. The Madagascar periwinkle [Catharanthus roseus (L.) G. Don] is a commercially important horticultural flower species and is a valuable source for several monoterpenoid indole alkaloids (MIAs), such as the powerful antihypertensive drug ajmalicine and the antineoplastic agents, vinblastine and vincristine...
November 17, 2017: Planta
https://www.readbyqxmd.com/read/29147098/purification-and-anti-pathogenic-properties-of-immunoglobulin-concentrates-from-porcine-blood
#19
Tae-Hwan Jung, Jae-Hwan Choi, Kyung-Chul Koh, Woo-Min Jeon, Kyoung-Sik Han
During slaughtering, animal blood is typically discarded, resulting in water pollution. However, this discarded blood has valuable components, such as immunoglobulin (Ig). Although several studies have been conducted to develop methods for effective recycling of slaughterhouse blood, they have not been commercially utilized in Korea. Here, we extracted an Ig-rich fraction from porcine blood that was then subjected to various in vitro tests, including pathogen growth inhibition, antigenic cross-reactivity, and anti-toxin activity...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/29147011/lower-serum-extracellular-superoxide-dismutase-levels-are-associated-with-polyneuropathy-in-recent-onset-diabetes
#20
Alexander Strom, Kirti Kaul, Jutta Brüggemann, Iris Ziegler, Ilka Rokitta, Sonja Püttgen, Julia Szendroedi, Karsten Müssig, Michael Roden, Dan Ziegler
Increased oxidative stress is implicated in the pathogenesis of experimental diabetic neuropathy, but translational evidence in recent-onset diabetes is scarce. We aimed to determine whether markers of systemic oxidative stress are associated with diabetic sensorimotor polyneuropathy (DSPN) in recent-onset diabetes. In this cross-sectional study, we measured serum concentrations of extracellular superoxide dismutase (SOD3), thiobarbituric acid reactive substances (TBARS), and reduced glutathione (GSH) in 107 type 1 and 215 type 2 diabetes patients from the German Diabetes Study baseline cohort and 37 glucose-tolerant individuals (controls)...
November 17, 2017: Experimental & Molecular Medicine
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