keyword
MENU ▼
Read by QxMD icon Read
search

Microglia actin migration

keyword
https://www.readbyqxmd.com/read/27714837/extracellular-atp-induces-graded-reactive-response-of-astrocytes-and-strengthens-their-antioxidative-defense-in-vitro
#1
Marija Adzic, Ivana Stevanovic, Natasa Josipovic, Danijela Laketa, Irena Lavrnja, Ivana M Bjelobaba, Iva Bozic, Marija Jovanovic, Milena Milosevic, Nadezda Nedeljkovic
It is widely accepted that adenosine triphosphate (ATP) acts as a universal danger-associated molecular pattern with several known mechanisms for immune cell activation. In the central nervous system, ATP activates microglia and astrocytes and induces a neuroinflammatory response. The aim of the present study was to describe responses of isolated astrocytes to increasing concentrations of ATP (5 µM to 1 mM), which were intended to mimic graded intensity of the extracellular stimulus. The results show that ATP induces graded activation response of astrocytes in terms of the cell proliferation, stellation, shape remodeling, and underlying actin and GFAP filament rearrangement, although the changes occurred without an apparent increase in GFAP and actin protein expression...
October 7, 2016: Journal of Neuroscience Research
https://www.readbyqxmd.com/read/26441534/microglia-mechanics-immune-activation-alters-traction-forces-and-durotaxis
#2
Lars Bollmann, David E Koser, Rajesh Shahapure, Hélène O B Gautier, Gerhard A Holzapfel, Giuliano Scarcelli, Malte C Gather, Elke Ulbricht, Kristian Franze
Microglial cells are key players in the primary immune response of the central nervous system. They are highly active and motile cells that chemically and mechanically interact with their environment. While the impact of chemical signaling on microglia function has been studied in much detail, the current understanding of mechanical signaling is very limited. When cultured on compliant substrates, primary microglial cells adapted their spread area, morphology, and actin cytoskeleton to the stiffness of their environment...
2015: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/25989853/actin-dynamics-shape-microglia-effector-functions
#3
Ria Uhlemann, Karen Gertz, Wolfgang Boehmerle, Tobias Schwarz, Christiane Nolte, Dorette Freyer, Helmut Kettenmann, Matthias Endres, Golo Kronenberg
Impaired actin filament dynamics have been associated with cellular senescence. Microglia, the resident immune cells of the brain, are emerging as a central pathophysiological player in neurodegeneration. Microglia activation, which ranges on a continuum between classical and alternative, may be of critical importance to brain disease. Using genetic and pharmacological manipulations, we studied the effects of alterations in actin dynamics on microglia effector functions. Disruption of actin dynamics did not affect transcription of genes involved in the LPS-triggered classical inflammatory response...
June 2016: Brain Structure & Function
https://www.readbyqxmd.com/read/25825709/%C3%AE-synuclein-a-chemoattractant-directs-microglial-migration-via-h2o2-dependent-lyn-phosphorylation
#4
Shijun Wang, Chun-Hsien Chu, Tessandra Stewart, Carmen Ginghina, Yifei Wang, Hui Nie, Mingri Guo, Belinda Wilson, Jau-Shyong Hong, Jing Zhang
Malformed α-Synuclein (α-syn) aggregates in neurons are released into the extracellular space, activating microglia to induce chronic neuroinflammation that further enhances neuronal damage in α-synucleinopathies, such as Parkinson's disease. The mechanisms by which α-syn aggregates activate and recruit microglia remain unclear, however. Here we show that α-syn aggregates act as chemoattractants to direct microglia toward damaged neurons. In addition, we describe a mechanism underlying this directional migration of microglia...
April 14, 2015: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/25600517/scutellarin-regulates-the-notch-pathway-and-affects-the-migration-and-morphological-transformation-of-activated-microglia-in-experimentally-induced-cerebral-ischemia-in-rats-and-in-activated-bv-2-microglia
#5
Yun Yuan, Parakalan Rangarajan, Enci Mary Kan, Yajun Wu, Chunyun Wu, Eng-Ang Ling
BACKGROUND: Activated microglial cells release an excess of inflammatory mediators after an ischemic stroke. We reported previously that scutellarin effectively suppressed the inflammatory response induced by activated microglia in rats subjected to middle cerebral artery occlusion (MCAO); however, the mechanism via which scutellarin exerts its effects on microglial activation has not been explored. This study aimed to elucidate if scutellarin can regulate the Notch pathway that is linked to microglia activation in MCAO rat, and in lipopolysaccharide (LPS)-induced BV-2 microglia...
January 20, 2015: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/25150163/a-pivotal-role-of-nonmuscle-myosin-ii-during-microglial-activation
#6
Stefanie Janßen, Viktoria Gudi, Chittappen K Prajeeth, Vikramjeet Singh, Katharina Stahl, Sandra Heckers, Thomas Skripuletz, Refik Pul, Corinna Trebst, Georgios Tsiavaliaris, Martin Stangel
Microglia are resident macrophages in the central nervous system (CNS) and the primary cells that contribute to CNS inflammation in many pathological conditions. Upon any signs of brain damage, microglia become activated and undergo tremendous cellular reorganization to adopt appropriate phenotypes. They migrate to lesion areas, accumulate, phagocytose cells or cellular debris, and produce a large array of inflammatory mediators like cytokines, chemokines, reactive oxygen species, and other mediators. To cope with the extreme cellular rearrangements during activation, microglia have to be highly dynamic...
November 2014: Experimental Neurology
https://www.readbyqxmd.com/read/25043721/contribution-of-sodium-channels-to-lamellipodial-protrusion-and-rac1-and-erk1-2-activation-in-atp-stimulated-microglia
#7
Anna-Karin Persson, Mark Estacion, Hyesook Ahn, Shujun Liu, Severine Stamboulian-Platel, Stephen G Waxman, Joel A Black
Microglia are motile resident immune cells of the central nervous system (CNS) that continuously explore their territories for threats to tissue homeostasis. Following CNS insult (e.g., cellular injury, infection, or ischemia), microglia respond to signals such as ATP, transform into an activated state, and migrate towards the threat. Directed migration is a complex and highly-coordinated process involving multiple intersecting cellular pathways, including signal transduction, membrane adhesion and retraction, cellular polarization, and rearrangement of cytoskeletal elements...
December 2014: Glia
https://www.readbyqxmd.com/read/23866683/diverse-activation-of-microglia-by-chemokine-c-c-motif-ligand-2-overexpression-in-brain
#8
Maj-Linda B Selenica, Jennifer A Alvarez, Kevin R Nash, Daniel C Lee, Chuanhai Cao, Xiaoyang Lin, Patrick Reid, Peter R Mouton, Dave Morgan, Marcia N Gordon
BACKGROUND: The chemokine (C-C motif) ligand 2 (CCL2) is a monocyte chemoattractant protein that mediates macrophage recruitment and migration during peripheral and central nervous system (CNS) inflammation. METHODS: To determine the impact of CCL2 in inflammation in vivo and to elucidate the CCL2-induced polarization of activated brain microglia, we delivered CCL2 into the brains of wild-type mice via recombinant adeno-associated virus serotype 9 (rAAV-9) driven by the chicken β-actin promoter...
2013: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/23851187/caveolin-isoform-switching-as-a-molecular-structural-and-metabolic-regulator-of-microglia
#9
Ingrid R Niesman, Nathan Zemke, Heidi N Fridolfsson, Kristofer J Haushalter, Karen Levy, Anna Grove, Rosalie Schnoor, J Cameron Finley, Piyush M Patel, David M Roth, Brian P Head, Hemal H Patel
Microglia are ramified cells that serve as central nervous system (CNS) guardians, capable of proliferation, migration, and generation of inflammatory cytokines. In non-pathological states, these cells exhibit ramified morphology with processes intermingling with neurons and astrocytes. Under pathological conditions, they acquire a rounded amoeboid morphology and proliferative and migratory capabilities. Such morphological changes require cytoskeleton rearrangements. The molecular control points for polymerization states of microtubules and actin are still under investigation...
September 2013: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/23649635/function-of-ezrin-radixin-moesin-proteins-in-migration-of-subventricular-zone-derived-neuroblasts-following-traumatic-brain-injury
#10
Younghye Moon, Joo Yeon Kim, Woon Ryoung Kim, Hyun Jung Kim, Min Jee Jang, Yoonkey Nam, Kyungjin Kim, Hyun Kim, Woong Sun
Throughout life, newly generated neuroblasts from the subventricular zone migrate toward the olfactory bulb through the rostral migratory stream. Upon brain injury, these migrating neuroblasts change their route and begin to migrate toward injured regions, which is one of the regenerative responses after brain damage. This injury-induced migration is triggered by stromal cell-derived factor 1 (SDF1) released from microglia near the damaged site; however, it is still unclear how these cells transduce SDF1 signals and change their direction...
August 2013: Stem Cells
https://www.readbyqxmd.com/read/23586020/integrins-in-trabecular-meshwork-and-optic-nerve-head-possible-association-with-the-pathogenesis-of-glaucoma
#11
REVIEW
Yisheng Zhong, Jing Wang, Xunda Luo
Integrins are a family of membrane-spanning proteins that are important receptors for cell adhesion to extracellular matrix proteins. They also provide connections between the extracellular environment and intracellular cytoskeletons and are responsible for activation of many intracellular signaling pathways. In vitro and in vivo data strongly indicate that integrin-mediated signaling events can modulate the organization of the actin cytoskeleton in trabecular meshwork (TM) cells and are associated with astrocyte migration and microglia activation of the optic nerve head in patients with primary open angle glaucoma...
2013: BioMed Research International
https://www.readbyqxmd.com/read/23499849/interaction-of-glia-with-a-compliant-microstructured-silicone-surface
#12
Ivan R Minev, Pouria Moshayedi, James W Fawcett, Stéphanie P Lacour
Soft bioengineered surfaces offer a route towards modulating the tissue responses to chronically implanted devices and may enhance their functionality. In this communication we fabricate microtopographically rich and mechanically compliant silicone surfaces for use in soft neural interfaces. We observe the interaction of primary rat microglia and astroglia with arrays of tall and short (4.7 and 0.5μm) vertically oriented polydimethylsiloxane (PDMS) micropillars and a flat PDMS surface in vitro. With the pillar size and spacing that we use (1...
June 2013: Acta Biomaterialia
https://www.readbyqxmd.com/read/23440885/radixin-expression-in-microglia-after-cortical-stroke-lesion
#13
Åsa Persson, Ahmed Osman, Hayde Bolouri, Carina Mallard, H Georg Kuhn
Stroke induces extensive tissue remodeling, resulting in the activation of several cell types in the brain as well as recruitment of blood-borne leucocytes. Radixin is part of a cytoskeleton linker protein family with the ability to connect transmembrane proteins to the actin cytoskeleton, promoting cell functions involving a dynamic cytoskeleton such as morphological changes, cell division and migration which are common events of different cell types after stroke. In the healthy adult brain radixin is expressed in Olig2(+) cells throughout the brain and in neural progenitor cells in the subventricular zone...
May 2013: Glia
https://www.readbyqxmd.com/read/23292072/nonmuscle-myosin-light-chain-kinase-mediates-microglial-migration-induced-by-hiv-tat-involvement-of-%C3%AE-1-integrins
#14
Honghong Yao, Ming Duan, Lu Yang, Shilpa Buch
One of the hallmark features of HIV-associated neurological disease is increased activation and migration of microglia. HIV transactivator of transcription (Tat) is released from infected cells and has the ability to recruit microglia. The purpose of this study was to investigate molecular mechanisms by which recombinant Tat₁₋₇₂, but not heated-inactive Tat₁₋₇₂,induces migration of rat primary microglia. Using primary microglia in Boyden chambers, we demonstrated the role of nonmuscle myosin light-chain kinase (nmMYLK) in Tat₁₋₇₂ (14...
April 2013: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/23160193/mutant-huntingtin-impairs-immune-cell-migration-in-huntington-disease
#15
Wanda Kwan, Ulrike Träger, Dimitrios Davalos, Austin Chou, Jill Bouchard, Ralph Andre, Aaron Miller, Andreas Weiss, Flaviano Giorgini, Christine Cheah, Thomas Möller, Nephi Stella, Katerina Akassoglou, Sarah J Tabrizi, Paul J Muchowski
In Huntington disease (HD), immune cells are activated before symptoms arise; however, it is unclear how the expression of mutant huntingtin (htt) compromises the normal functions of immune cells. Here we report that primary microglia from early postnatal HD mice were profoundly impaired in their migration to chemotactic stimuli, and expression of a mutant htt fragment in microglial cell lines was sufficient to reproduce these deficits. Microglia expressing mutant htt had a retarded response to a laser-induced brain injury in vivo...
December 2012: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/23158496/regulation-of-podosome-formation-microglial-migration-and-invasion-by-ca-2-signaling-molecules-expressed-in-podosomes
#16
Tamjeed A Siddiqui, Starlee Lively, Catherine Vincent, Lyanne C Schlichter
BACKGROUND: Microglia migrate during brain development and after CNS injury, but it is not known how they degrade the extracellular matrix (ECM) to accomplish this. Podosomes are tiny structures with the unique ability to adhere to and dissolve ECM. Podosomes have a two-part architecture: a core that is rich in F-actin and actin-regulatory molecules (for example, Arp2/3), surrounded by a ring with adhesion and structural proteins (for example, talin, vinculin). We recently discovered that the lamellum at the leading edge of migrating microglia contains a large F-actin-rich superstructure ('podonut') composed of many podosomes...
November 17, 2012: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/22873355/podosomes-in-migrating-microglia-components-and-matrix-degradation
#17
Catherine Vincent, Tamjeed A Siddiqui, Lyanne C Schlichter
BACKGROUND: To perform their functions during development and after central nervous system injury, the brain's immune cells (microglia) must migrate through dense neuropil and extracellular matrix (ECM), but it is not known how they degrade the ECM. In several cancer cell lines and peripheral cells, small multi-molecular complexes (invadopodia in cancer cells, podosomes in nontumor cells) can both adhere to and dissolve the ECM. Podosomes are tiny multi-molecular structures (0.4 to 1 μm) with a core, rich in F-actin and its regulatory molecules, surrounded by a ring containing adhesion and structural proteins...
2012: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/21451358/induction-of-ezrin-radixin-moesin-molecules-after-cryogenic-traumatic-brain-injury-of-the-mouse-cortex
#18
Younghye Moon, Joo Yeon Kim, So Yoen Choi, Kyungjin Kim, Hyun Kim, Woong Sun
Traumatic brain injury promotes rapid induction of microglial cells and infiltration of peripheral macrophages to the injury sites. Such inflammatory responses are mediated by the activation and migration of immune cells, which are influenced by the actin cytoskeleton remodeling. In this study, we observed that the phosphorylation and expressions of ezrin-radixin-moesin (ERM) proteins, which are linkers for cell surface with actin cytoskeleton, are induced in the activated microglia/macrophages, whereas ERM molecules are only marginally expressed in quiescent microglia in the normal brain...
April 20, 2011: Neuroreport
https://www.readbyqxmd.com/read/21442623/a-forward-loop-between-glioma-and-microglia-glioma-derived-extracellular-matrix-activated-microglia-secrete-il-18-to-enhance-the-migration-of-glioma-cells
#19
Wei-Lan Yeh, Dah-Yuu Lu, Houng-Chi Liou, Wen-Mei Fu
The mediators and cellular effectors of inflammation are important constituents of the local environment of tumors. In some occasions, oncogenic changes induce an inflammatory microenvironment that promotes the progression of tumors. In gliomas, the presence of microglia may represent tumor-related inflammation and microglia activation, and subsequent inflammatory responses may influence tumor growth and metastasis. Here, we found that C6 glioma--but not primary astrocyte-derived extracellular matrix (ECM) could activate microglia, including primary microglia and BV-2 cell line, and activated microglia-secreted interleukin (IL)-18, a potent inflammatory cytokine of the IL-1 family, to promote C6 migration...
February 2012: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/20435601/conditions-of-retinal-glial-and-inflammatory-cell-activation-after-irradiation-in-a-gfp-chimeric-mouse-model
#20
Philipp S Müther, Irina Semkova, Kristina Schmidt, Elizabeth Abari, Marc Kuebbeler, Marc Beyer, Hinrich Abken, Klaus L Meyer, Norbert Kociok, Antonia M Joussen
PURPOSE: Microglia cells have been associated with immunologic defense and repair. The course of retinal disease after lethal irradiation for bone marrow depletion and substitution was evaluated with respect to macrophage and microglial involvement. METHODS: Lethal irradiation in C57BL/6 mice was conducted with a low-voltage radiation unit. The animals were randomized to shielded or unshielded radiation and subsequently received transplants of GFP+ bone marrow cells (beta-actin promoter)...
September 2010: Investigative Ophthalmology & Visual Science
keyword
keyword
2538
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"