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https://www.readbyqxmd.com/read/28333992/improved-epidermal-barrier-formation-in-human-skin-models-by-chitosan-modulated-dermal-matrices
#1
Arnout Mieremet, Marion Rietveld, Samira Absalah, Jeroen van Smeden, Joke A Bouwstra, Abdoelwaheb El Ghalbzouri
Full thickness human skin models (FTMs) contain an epidermal and a dermal equivalent. The latter is composed of a collagen dermal matrix which harbours fibroblasts. Current epidermal barrier properties of FTMs do not fully resemble that of native human skin (NHS), which makes these human skin models less suitable for barrier related studies. To further enhance the resemblance of NHS for epidermal morphogenesis and barrier formation, we modulated the collagen dermal matrix with the biocompatible polymer chitosan...
2017: PloS One
https://www.readbyqxmd.com/read/28332875/the-biologic-basis-of-transgender-identity-2d-4d-finger-length-ratios-implicate-a-role-for-prenatal-androgen-activity
#2
Matthew Leinung, Christina Wu
Objective The biologic basis for gender identity is unknown. Research has shown that the ratio of the length of the second and fourth digits (2D:4D) in mammals is influenced by gender in utero, but data on 2D:4D ratios in transgender individuals are scarce and contradictory. We sought an association with 2D:4D ratio and gender identity in our transgender clinic population in Albany, NY. Methods We prospectively recruited 118 transgender subjects undergoing hormonal therapy (50 female to male (FTM) and 68 male to female (MTF) for finger length measurement...
March 23, 2017: Endocrine Practice
https://www.readbyqxmd.com/read/28297070/elbow-avf-configurations-and-indications
#3
Selcuk Baktiroglu, Fatih Yanar, Sercan Yuksel, Burak Celik, Halime G Kilic
INTRODUCTION: Brescia-Cimino radiocephalic arteriovenous fistula (AVF) remains the first-choice vascular access procedure for patients in need of long-term hemodialysis. The average life expectancy of patients receiving hemodialysis has increased in recent years and many patients now live longer and require secondary or tertiary procedures. Elbow fistulas should only rarely be constructed as primary fistulas. The aim of the surgeon must be not only to achieve a functioning fistula, but to avoid possible complications other than failure to mature (FTM), like distal ischemia and cardiac failure and to save the vessels as much as possible for future procedures...
March 6, 2017: Journal of Vascular Access
https://www.readbyqxmd.com/read/28297050/preoperative-computer-simulation-for-planning-of-vascular-access-surgery-in-hemodialysis-patients
#4
Niek Zonnebeld, Wouter Huberts, Magda M van Loon, Tammo Delhaas, Jan H M Tordoir
INTRODUCTION: The arteriovenous fistula (AVF) is the preferred vascular access for hemodialysis patients. Unfortunately, 20-40% of all constructed AVFs fail to mature (FTM), and are therefore not usable for hemodialysis. AVF maturation importantly depends on postoperative blood volume flow. Predicting patient-specific immediate postoperative flow could therefore support surgical planning. A computational model predicting blood volume flow is available, but the effect of blood flow predictions on the clinical endpoint of maturation (at least 500 mL/min blood volume flow, diameter of the venous cannulation segment ≥4 mm) remains undetermined...
March 6, 2017: Journal of Vascular Access
https://www.readbyqxmd.com/read/28224311/prevalence-incidence-and-sex-ratio-of-transsexualism-in-the-autonomous-region-of-madrid-spain-according-to-healthcare-demand
#5
Antonio Becerra-Fernández, José Miguel Rodríguez-Molina, Nuria Asenjo-Araque, María Jesús Lucio-Pérez, Miguel Cuchí-Alfaro, Eduardo García-Camba, Gilberto Pérez-López, Miriam Menacho-Román, María Carmen Berrocal-Sertucha, Domingo Ly-Pen, María Victorina Aguilar-Vilas
In recent years, different studies have provided estimates of the prevalence of transsexualism with very diverse results. The purpose of this study was to ascertain the prevalence, incidence, and sex ratio of transsexualism in the autonomous region of Madrid (Spain). A total of 1234 patients who attended from 2007 to the end of 2015 in the only Gender Identity Unit (GIU) in Madrid were analyzed. Sixty-three patients were excluded for various reasons; thus, 1171 could be included: 803 male-to-female (MtF) and 368 female-to-male (FtM) transsexual patients...
February 21, 2017: Archives of Sexual Behavior
https://www.readbyqxmd.com/read/28202061/in-vitro-generation-of-sertoli-like-and-haploid-spermatid-like-cells-from-human-umbilical-cord-perivascular-cells
#6
Ekaterina Shlush, Leila Maghen, Sonja Swanson, Shlomit Kenigsberg, Sergey Moskovtsev, Tanya Barretto, Andrée Gauthier-Fisher, Clifford L Librach
BACKGROUND: First trimester (FTM) and term human umbilical cord-derived perivascular cells (HUCPVCs), which are rich sources of mesenchymal stem cells (MSCs), can give rise to Sertoli cell (SC)-like as well as haploid germ cell (GC)-like cells in vitro using culture conditions that recapitulate the testicular niche. Gamete-like cells have been produced ex vivo using pluripotent stem cells as well as MSCs. However, the production of functional gametes from human stem cells has yet to be achieved...
February 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28175969/masculine-chest-wall-contouring-in-ftm-transgender-a-personal-approach
#7
Giulia Lo Russo, Sara Tanini, Marco Innocenti
BACKGROUND: Chest-wall contouring surgery is one of the first steps in sexual reassignment in female-to-male (FtM) transsexuals that contributes to strengthening of the self-image and facilitates living in the new gender role. The main goal is to masculinize the chest by removing the female contour. Chest contour, scar placement, scar shape, scar length, nipple-areola position, nipple size and the areola size are the key points. METHODS: Between July 2013 and June 2016, 25 FtM transgender patients underwent surgical procedures to create a masculine chest-wall contour...
February 7, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28117266/genotypes-and-haplotypes-of-the-estrogen-receptor-%C3%AE-gene-esr1-are-associated-with-female-to-male-gender-dysphoria
#8
Joselyn Cortés-Cortés, Rosa Fernández, Nerea Teijeiro, Esther Gómez-Gil, Isabel Esteva, Mari Cruz Almaraz, Antonio Guillamón, Eduardo Pásaro
INTRODUCTION: Gender dysphoria, a marked incongruence between one's experienced gender and biological sex, is commonly believed to arise from discrepant cerebral and genital sexual differentiation. With the discovery that estrogen receptor β is associated with female-to-male (FtM) but not with male-to-female (MtF) gender dysphoria, and given estrogen receptor α involvement in central nervous system masculinization, it was hypothesized that estrogen receptor α, encoded by the ESR1 gene, also might be implicated...
January 20, 2017: Journal of Sexual Medicine
https://www.readbyqxmd.com/read/28113374/unsupervised-3d-local-feature-learning-by-circle-convolutional-restricted-boltzmann-machine
#9
Zhizhong Han, Zhenbao Liu, Junwei Han, Chi-Man Vong, Shuhui Bu, Xuelong Li
Extracting local features from 3D shapes is an important and challenging task that usually requires carefully designed 3D shape descriptors. However, these descriptors are hand-crafted and require intensive human intervention with prior knowledge. To tackle this issue, we propose a novel deep learning model, namely Circle Convolutional Restricted Boltzmann Machine (CCRBM), for unsupervised 3D local feature learning. CCRBM is specially designed to learn from raw 3D representations. It effectively overcomes obstacles such as irregular vertex topology, orientation ambiguity on the 3D surface, and rigid or slightly non-rigid transformation invariance in the hierarchical learning of 3D data that cannot be resolved by the existing deep learning models...
September 2, 2016: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28062908/role-of-altered-cerebello-thalamo-cortical-network-in-the-neurobiology-of-essential-tremor
#10
Abhishek Lenka, Ketaki Swapnil Bhalsing, Rajanikant Panda, Ketan Jhunjhunwala, Rajini M Naduthota, Jitender Saini, Rose Dawn Bharath, Ravi Yadav, Pramod Kumar Pal
INTRODUCTION: Essential tremor (ET) is the most common movement disorder among adults. Although ET has been recognized as a mono-symptomatic benign illness, reports of non-motor symptoms and non-tremor motor symptoms have increased its clinical heterogeneity. The neural correlates of ET are not clearly understood. The aim of this study was to understand the neurobiology of ET using resting state fMRI. METHODS: Resting state functional MR images of 30 patients with ET and 30 age- and gender-matched healthy controls were obtained...
January 6, 2017: Neuroradiology
https://www.readbyqxmd.com/read/27904649/fasciocutaneous-flap-reinforcement-of-ventral-onlay-buccal-mucosa-grafts-enables-neophallus-revision-urethroplasty
#11
Stelios C Wilson, John T Stranix, Kiranpreet Khurana, Shane D Morrison, Jamie P Levine, Lee C Zhao
BACKGROUND: Urethral strictures or fistulas are common complications after phalloplasty. Neourethral defects pose a difficult reconstructive challenge using standard techniques as there is generally insufficient ventral tissue to support a graft urethroplasty. We report our experience with local fasciocutaneous flaps for support of ventrally-placed buccal mucosal grafts (BMGs) in phalloplasty. METHODS: A retrospective review of patients who underwent phalloplasty and subsequently required revision urethroplasty using BMGs between 2011 and 2015 was completed...
December 2016: Therapeutic Advances in Urology
https://www.readbyqxmd.com/read/27854157/measuring-fecal-testosterone-metabolites-in-spotted-hyenas-choosing-the-wrong-assay-may-lead-to-erroneous-results
#12
Susanne Pribbenow, Tulsidas G Shrivastav, Martin Dehnhard
Enzyme-immunoassays (EIA) that detect fecal testosterone metabolites (fTM) are powerful tools to monitor gonadal activity non-invasively. However, a challenge with testosterone EIAs might be their potential for cross-reactivities with structurally similar glucocorticoid metabolites. Therefore, we aimed to verify the capability of four different testosterone EIAs to monitor fTM without reflecting changes in adrenocortical activity in spotted hyenas by analyzing fecal samples following testosterone and ACTH challenge tests...
November 17, 2016: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27844235/robot-assisted-laparoscopic-colpectomy-in-female-to-male-transgender-patients-technique-and-outcomes-of-a-prospective-cohort-study
#13
Freek Groenman, Charlotte Nikkels, Judith Huirne, Mick van Trotsenburg, Hans Trum
BACKGROUND: Gender-affirming surgeries in female-to-male (FtM) transgender patients include mostly hysterectomy, bilateral salpingo-oophorectomy and mastectomy. Sometimes further surgery is performed, such as phalloplasty. Colpectomy may be performed to overcome gender dysphoria and disturbing vaginal discharge; furthermore, it may be important in reducing the risk of fistulas due to the phalloplasty procedure with urethral elongation. Colpectomy prior to the reconstruction of the neourethra seems to reduce fistula rates on the very first anastomosis...
November 14, 2016: Surgical Endoscopy
https://www.readbyqxmd.com/read/27818844/a-pilot-study-of-botulinum-toxin-for-jerky-position-specific-upper-limb-action-tremor
#14
Tabish A Saifee, Tiago Teodoro, Roberto Erro, Mark J Edwards, Carla Cordivari
BACKGROUND: We aimed to investigate the efficacy and safety of botulinum toxin (BT) injections for jerky action tremor of the upper limb. METHODS: We performed an uncontrolled, prospective study of electromyography (EMG)-guided BT injections for jerky, position-specific, upper limb action tremor. The primary outcome was clinical global impression at 3-6 weeks after baseline. RESULTS: Eight patients with jerky, position-specific action tremor involving the upper limb were consecutively recruited...
2016: Tremor and Other Hyperkinetic Movements
https://www.readbyqxmd.com/read/27815157/excretion-and-measurement-of-corticosterone-and-testosterone-metabolites-in-bank-voles-myodes-glareolus
#15
Saana Sipari, Hannu Ylönen, Rupert Palme
The bank vole is a commonly used model species in behavioral and ecophysiological studies. Thus, presenting a validated method for noninvasive monitoring of corticosterone and testosterone secretion is of high relevance. Here, we evaluated the effect of time of day and an ACTH challenge test on measured fecal corticosterone (FCM) and testosterone (FTM) metabolites in both sexes. Furthermore, we performed radiometabolism experiments for both steroids and sexes to study metabolism and excretion of (3)H-corticosterone and (3)H-testosterone...
March 1, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/27780883/human-umbilical-perivascular-cells-a-novel-source-of-mscs-to-support-testicular-niche-regeneration
#16
Leila Maghen, Ekaterina Shlush, Itai Gat, Melissa Filice, Tanya Barretto, Keith Jarvi, Kirk Lo, Andree S Gauthier-Fisher, Clifford L Librach
The expansion of functional testicular biopsy-derived human spermatogonial stem cells (hSSC) ex-vivo may enable the restoration of fertility in pre-pubertal males having undergone gonadotoxic therapies or men with severe male factor infertility. Various somatic cells are known to regulate SSC homeostasis and spermatogenesis in the developing and adult testis. Prior attempts to recapitulate this niche demonstrated the requirement of feeder cells, such as endogenous testicular somatic cells, for germ cell expansion ex-vivo...
October 25, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27744092/subcortical-gray-matter-changes-in-transgender-subjects-after-long-term-cross-sex-hormone-administration
#17
Rene Seiger, Andreas Hahn, Allan Hummer, Georg S Kranz, Sebastian Ganger, Michael Woletz, Christoph Kraus, Ronald Sladky, Alexander Kautzky, Siegfried Kasper, Christian Windischberger, Rupert Lanzenberger
Sex-steroid hormones are primarily involved in sexual differentiation and development and are thought to underlie processes related to cognition and emotion. However, divergent results have been reported concerning the effects of hormone administration on brain structure including side effects like brain atrophy and dementia. Cross-sex hormone therapy in transgender subjects offers a unique model for studying the effects of sex hormones on the living human brain. In this study, 25 Female-to-Male (FtM) and 14 Male-to-Female (MtF) subjects underwent MRI examinations at baseline and after a period of at least 4-months of continuous cross-sex hormone administration...
December 2016: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/27742823/the-variable-target-model-a-paradigm-shift-in-the-incremental-haemodialysis-prescription
#18
Francesco Gaetano Casino, Carlo Basile
BACKGROUND: The recent interest in incremental haemodialysis (HD) is hindered by the current prescription based on a fixed target model (FTM) for the total (dialytic + renal) equivalent continuous clearance (ECC). The latter is expressed either as standard Kt/V (stdKt/V), i.e. the pre-dialysis averaged concentration of urea-based ECC, or EKRc, i.e. the time averaged concentration-based ECC, corrected for volume (V) = 40 L. Accordingly, there are two different targets: stdKt/V = 2.3 volumes per week (v/wk) and EKRc = 13 mL/min/40 L...
October 14, 2016: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/27705385/mapping-discrimination-experienced-by-indonesian-trans-ftm-persons
#19
Danny Gordon, Mario Prajna Pratama
This work sought to document how Indonesian trans* FtM persons experienced discrimination across the interlinked domains of social networks, religious and educational institutions, employment and the workplace, and health care institutions. Objectives were (1) to map the discrimination experienced by trans* FtM individuals in Indonesia, and (2) to establish the specific priorities of the Indonesian trans* FtM community. In-depth interviews, focus groups, and participant observation was used involving 14 respondents...
October 5, 2016: Journal of Homosexuality
https://www.readbyqxmd.com/read/27686883/patterns-of-sexual-behaviour-and-sexual-healthcare-needs-among-transgender-individuals-in-melbourne-australia-2011-2014
#20
Clare Bellhouse, Sandra Walker, Christopher K Fairley, Lenka A Vodstrcil, Catriona S Bradshaw, Marcus Y Chen, Eric P F Chow
OBJECTIVE: Literature surrounding the healthcare needs of transgender individuals is limited in Australia. This study aimed to investigate the demographic characteristics, risk behaviours and HIV/STI positivity among male-to-female (MTF) and female-to-male (FTM) transgender individuals attending Melbourne Sexual Health Centre (MSHC), Australia, between 2011 and 2014. METHOD: A retrospective cohort analysis for 133 transgender individuals was conducted based on the first visit of individuals to MSHC during the study period...
September 29, 2016: Sexually Transmitted Infections
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