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https://www.readbyqxmd.com/read/27898896/environmental-morphological-and-productive-characterization-of-sardinian-goats-and-use-of-latent-explanatory-factors-for-population-analysis
#1
G M Vacca, P Paschino, M L Dettori, M Bergamaschi, C Cipolat-Gotet, G Bittante, M Pazzola
Dairy goat farming is practiced worldwide, within a range of different farming systems. Here we investigated the effects of environmental factors and morphology on milk traits of the Sardinian goat population. Sardinian goats are currently reared in Sardinia (Italy) in a low-input context, similar to many goat farming systems, especially in developing countries. Milk and morphological traits from 1,050 Sardinian goats from 42 farms were recorded. We observed a high variability regarding morphological traits, such as coat color, ear length and direction, horn presence, and udder shape...
September 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27890773/primary-photosensitization-caused-by-ingestion-of-froelichia-humboldtiana-by-dairy-goats
#2
Danielle S Santos, Caio C B Silva, Valber O Araújo, Maria de Fátima Souza, Patrícia B Lacerda-Lucena, Sara V D Simões, Franklin Riet-Correa, Ricardo B Lucena
This study aimed to describe the first report of an outbreak of primary photosensitization in dairy goats caused by Froelichia humboldtiana. The disease occurred from March to May 2014, in a mixed flock of 15 Saanen, Alpine, and crossbred lactating goats. The entire flock was affected; however, the Saanen and cross-bred goats developed more severe lesions, and their milk production decreased by as much as 75%. Lesions were progressive and consisted of skin hyperemia, edema, and necrosis, especially in depigmented areas...
November 24, 2016: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/27889169/characterization-of-biocide-tolerant-bacteria-isolated-from-cheese-and-dairy-small-medium-enterprises
#3
Ma Luisa Fernández Márquez, Ma José Grande Burgos, Ma Carmen López Aguayo, Rubén Pérez Pulido, Antonio Gálvez, Rosario Lucas
A collection of 120 bacterial isolates from small medium enterprises involved in the production of cow milk and the manufacture of goat cheese were screened for sensitivity to biocides benzalkonium chloride (BC), cetrimide (CT), hexadecylpyridinium chloride (HDP), triclosan (TC), hexachlorophene (CF) and poly-(hexamethylen guanidinium) hydrochloride (PHMG). Nineteen isolates were selected according to biocide tolerance and identified by 16S rDNA sequencing as Lactococcus sp. (6) Enterococcus sp. (1), Lactobacillus sp...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27889164/multilocus-sequence-type-profiles-of-bacillus-cereus-isolates-from-infant-formula-in-china
#4
Yong Yang, Xiaofeng Yu, Li Zhan, Jiancai Chen, Yunyi Zhang, Junyan Zhang, Honghu Chen, Zheng Zhang, Yanjun Zhang, Yiyu Lu, Lingling Mei
Bacillus cereus sensu stricto is an opportunistic foodborne pathogen. The multilocus sequence type (MLST) of 74 B. cereus isolated from 513 non-random infant formula in China was analyzed. Of 64 sequence types (STs) detected, 50 STs and 6 alleles were newly found in PubMLST database. All isolates except for one singleton (ST-1049), were classified into 7 clonal complexes (CC) by BURST (n-4), in which CC1 with core ancestral clone ST-26 was the largest group including 86% isolates, and CC2, 3, 9, 10 and 13 were first reported in China...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27884183/rna-sequencing-for-profiling-goat-milk-transcriptome-in-colostrum-and-mature-milk
#5
Alessandra Crisà, Fabrizio Ferrè, Giovanni Chillemi, Bianca Moioli
BACKGROUND: In this work we aimed at sequencing and assembling the goat milk transcriptome corresponding at colostrum and 120 days of lactation. To reconstruct transcripts we used both the genome as reference, and a de novo assembly approach. Additionally, we aimed at identifying the differentially expressed genes (DEGs) between the two lactation stages and at analyzing the expression of genes involved in oligosaccharides metabolism. RESULTS: A total of 44,635 different transcripts, organized in 33,757 tentative genes, were obtained using the goat genome as reference...
November 25, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27865506/synthesis-of-the-suspected-trans-11-cis-13-conjugated-linoleic-acid-isomer-in-ruminant-mammary-tissue-by-fads3-catalyzed-%C3%AE-13-desaturation-of-vaccenic-acid
#6
Cyrielle Garcia, Cécile Duby, Daniel Catheline, Pablo G Toral, Laurence Bernard, Philippe Legrand, Vincent Rioux
The octadecadienoic conjugated linoleic acid (CLA) isomer with trans-11 and cis-13 double bonds (trans-11,cis-13 CLA) has been described in ruminant milk. For now, this specific CLA is suspected to derive exclusively from ruminal biohydrogenation of dietary α-linolenic acid. However, in rodents, the fatty acid desaturase 3 (FADS3) gene was recently shown to code for an enzyme able to catalyze the unexpected Δ13-desaturation of vaccenic acid, producing a Δ11,13-CLA with all the structural characteristics of the trans-11,cis-13 isomer, although no commercial standard exists for complete conclusive identification...
November 16, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27863483/estimation-of-tulathromycin-depletion-in-plasma-and-milk-after-subcutaneous-injection-in-lactating-goats-using-a-nonlinear-mixed-effects-pharmacokinetic-modeling-approach
#7
Zhoumeng Lin, Matthew Cuneo, Joan D Rowe, Mengjie Li, Lisa A Tell, Shayna Allison, Jan Carlson, Jim E Riviere, Ronette Gehring
BACKGROUND: Extra-label use of tulathromycin in lactating goats is common and may cause violative residues in milk. The objective of this study was to develop a nonlinear mixed-effects pharmacokinetic (NLME-PK) model to estimate tulathromycin depletion in plasma and milk of lactating goats. Eight lactating goats received two subcutaneous injections of 2.5 mg/kg tulathromycin 7 days apart; blood and milk samples were analyzed for concentrations of tulathromycin and the common fragment of tulathromycin (i...
November 18, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27857705/isolation-identification-and-characterization-of-yeasts-from-fermented-goat-milk-of-the-yaghnob-valley-in-tajikistan
#8
Linnea A Qvirist, Carlotta De Filippo, Francesco Strati, Irene Stefanini, Maddalena Sordo, Thomas Andlid, Giovanna E Felis, Paola Mattarelli, Duccio Cavalieri
The geographically isolated region of the Yaghnob Valley, Tajikistan, has allowed its inhabitants to maintain a unique culture and lifestyle. Their fermented goat milk constitutes one of the staple foods for the Yaghnob population, and is produced by backslopping, i.e., using the previous fermentation batch to inoculate the new one. This study addresses the yeast composition of the fermented milk, assessing genotypic, and phenotypic properties. The 52 isolates included in this study revealed small species diversity, belonging to Kluyveromyces marxianus, Pichia fermentans, Saccharomyces cerevisiae, and one Kazachstania unispora...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27855911/lipid-domains-in-the-milk-fat-globule-membrane-dynamics-investigated-in-situ-in-milk-in-relation-to-temperature-and-time
#9
Oumaima Et-Thakafy, Fanny Guyomarc'h, Christelle Lopez
The microstructure of the milk fat globule membrane (MFGM) is still poorly understood. The aim of this study was to investigate the dynamics of the MFGM at the surface of milk fat globules in relation to temperature and time, and in relation to the respective lipid compositions of the MFGM from bovine, goat and sheep milks. In-situ structural investigations were performed using confocal microscopy. Lipid domains were observed over a wide range of temperatures (4-60°C). We demonstrated that rapid cooling of milk enhances the mechanisms of nucleation and that extended storage induces lipid reorganization within the MFGM with growth, leading to circular lipid domains...
April 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27854329/mir-30e-5p-and-mir-15a-synergistically-regulate-fatty-acid-metabolism-in-goat-mammary-epithelial-cells-via-lrp6-and-yap1
#10
Zhi Chen, Huiling Qiu, Liuan Ma, Jun Luo, Shuang Sun, Kang Kang, Deming Gou, Juan J Loor
MicroRNA (miRNA) regulates the expression of genes and influences a series of biological processes, including fatty acid metabolism. We screened the expression of miRNA in goat mammary glands during peak-lactation and non-lactating ("dry") periods, and performed an in vitro study with goat mammary epithelial cells (GMEC) prior to sequencing analysis. Results illustrated that miR-30e-5p and miR-15a were highly expressed. Utilizing a luciferase reporter assay and Western blot, low-density lipoprotein receptor-related protein 6 (LRP6) and Yes associated protein 1 (YAP1) genes were demonstrated to be a target of miR-30e-5p and miR-15a in GMEC...
November 16, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27853711/aflatoxin-m1-levels-in-raw-milk-pasteurised-milk-and-infant-formula
#11
Sharaf S Omar
The incidence of contamination of aflatoxin M1 (AFM1) in milk samples collected from the Jordanian market was investigated by using the competitive enzyme linked immunosorbent assay (ELISA) technique. A total of 175 samples were collected during 2014-2015. All tested samples were contaminated with various levels of AFM1 ranging from 9.71 to 288.68 ng/kg. The concentration of AFM1 in 66% of fresh milk samples was higher than the maximum tolerance limit accepted by the European Union (50 ng/kg) and 23% higher than the maximum tolerance limit accepted by the US (500 ng/kg)...
June 3, 2016: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/27845025/effect-of-different-fat-replacers-on-the-physicochemical-and-instrumental-analysis-of-low-fat-cupuassu-goat-milk-yogurts
#12
Marion P Costa, Beatriz S Frasao, Bruna L Rodrigues, Adriana Co Silva, Carlos A Conte-Junior
The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP). Three batches of each cupuassu goat milk yogurt were prepared and analysed on the 1st day of storage by pH, proximate composition, colour, apparent viscosity, and texture. In comparison to yogurts from whole (W) or skimmed milk (S), all of the fat replacers improved the physicochemical properties (P < 0·05)...
November 2016: Journal of Dairy Research
https://www.readbyqxmd.com/read/27818678/transcriptome-wide-analysis-reveals-the-role-of-ppar%C3%AE-controlling-the-lipid-metabolism-in-goat-mammary-epithelial-cells
#13
Hengbo Shi, Wangsheng Zhao, Changhui Zhang, Khuram Shahzad, Jun Luo, Juan J Loor
To explore the large-scale effect of peroxisome proliferator-activated receptor γ (PPARG) in goat mammary epithelial cells (GMEC), an oligonucleotide microarray platform was used for transcriptome profiling in cells overexpressing PPARG and incubated with or without rosiglitazone (ROSI, a PPARγ agonist). A total of 1143 differentially expressed genes (DEG) due to treatment were detected. The Dynamic Impact Approach (DIA) analysis uncovered the most impacted and induced pathways "fatty acid elongation in mitochondria," "glycosaminoglycan biosynthesis-keratan sulfate," and "pentose phosphate pathway...
2016: PPAR Research
https://www.readbyqxmd.com/read/27816243/consumer-perception-health-information-and-instrumental-parameters-of-cupuassu-theobroma-grandiflorum-goat-milk-yogurts
#14
Marion P Costa, Maria Lucia G Monteiro, Beatriz S Frasao, Vitor L M Silva, Bruna L Rodrigues, Claudete C J Chiappini, Carlos A Conte-Junior
Although the demand for goat milk products has been growing, they have lower consumer acceptability when compared with products derived from cow milk. However, the addition of cupuassu pulp can be used to improve the formulation of these products. For this reason, the aim of this study was to investigate the influence of new goat milk yogurt manufactured with cupuassu pulp on physicochemical properties, consumers' perceptions, and overall consumer acceptance. In addition, the effect of antioxidant health information on consumer acceptance and purchase intention of cupuassu goat milk yogurts was evaluated...
November 2, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27814699/in-silico-identification-of-essential-proteins-in-corynebacterium-pseudotuberculosis-based-on-protein-protein-interaction-networks
#15
Edson Luiz Folador, Paulo Vinícius Sanches Daltro de Carvalho, Wanderson Marques Silva, Rafaela Salgado Ferreira, Artur Silva, Michael Gromiha, Preetam Ghosh, Debmalya Barh, Vasco Azevedo, Richard Röttger
BACKGROUND: Corynebacterium pseudotuberculosis (Cp) is a gram-positive bacterium that is classified into equi and ovis serovars. The serovar ovis is the etiological agent of caseous lymphadenitis, a chronic infection affecting sheep and goats, causing economic losses due to carcass condemnation and decreased production of meat, wool, and milk. Current diagnosis or treatment protocols are not fully effective and, thus, require further research of Cp pathogenesis. RESULTS: Here, we mapped known protein-protein interactions (PPI) from various species to nine Cp strains to reconstruct parts of the potential Cp interactome and to identify potentially essential proteins serving as putative drug targets...
November 4, 2016: BMC Systems Biology
https://www.readbyqxmd.com/read/27806722/recombinase-polymerase-amplification-assay-for-rapid-detection-of-lumpy-skin-disease-virus
#16
Mohamed A Shalaby, Ayman El-Deeb, Mohamed El-Tholoth, Donata Hoffmann, Claus-Peter Czerny, Frank T Hufert, Manfred Weidmann, Ahmed Abd El Wahed
BACKGROUND: Lumpy skin disease virus (LSDV) is a Capripoxvirus infecting cattle and Buffalos. Lumpy skin disease (LSD) leads to significant economic losses due to hide damage, reduction of milk production, mastitis, infertility and mortalities (10 %). Early detection of the virus is crucial to start appropriate outbreak control measures. Veterinarians rely on the presence of the characteristic clinical signs of LSD. Laboratory diagnostics including virus isolation, sequencing and real-time polymerase chain reaction (PCR) are performed at well-equipped laboratories...
November 2, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27796358/scanning-of-selection-signature-provides-a-glimpse-into-important-economic-traits-in-goats-capra-hircus
#17
Dailu Guan, Nanjian Luo, Xiaoshan Tan, Zhongquan Zhao, Yongfu Huang, Risu Na, Jiahua Zhang, Yongju Zhao
Goats (Capra hircus) are one of the oldest livestock domesticated species, and have been used for their milk, meat, hair and skins over much of the world. Detection of selection footprints in genomic regions can provide potential insights for understanding the genetic mechanism of specific phenotypic traits and better guide in animal breeding. The study presented here has generated 192.747G raw data and identified more than 5.03 million single-nucleotide polymorphisms (SNPs) and 334,151 Indels (insertions and deletions)...
October 31, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27791476/polymorphisms-of-the-toll-like-receptor-2-of-goats-capra-hircus-may-be-associated-with-somatic-cell-count-in-milk
#18
Christina T Ruiz-Rodriguez, Jessica R Brandt, Ryan Oliverio, Yasuko Ishida, Noa Guedj, Edgar F Garrett, Gila Kahila Bar-Gal, Nikolas Nikolaidis, Felipe C Cardoso, Alfred L Roca
Toll-like receptor 2 (TLR2) plays an important role in recognition by the innate immune system of Gram-positive bacteria. As Gram-positive bacteria cause mastitis, we examined variations in the region of the TLR2 gene that codes for the extracellular domain. Samples of forty goats from a single dairy herd were collected, half with low SCC (≤200,000 cells/mL), and half with higher SCC. Two synonymous single nucleotide polymorphisms (SNPs) were identified: 840G > A and 1083A > G. One nonsynonymous SNP 739G > A was identified...
October 28, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27790701/the-application-of-nmr-based-milk-metabolites-analysis-in-milk-authenticity-identification
#19
Qiangqiang Li, Zunbo Yu, Dan Zhu, Xianghe Meng, Xiumei Pang, Yue Liu, Russell Frew, He Chen, Gang Chen
BACKGROUND: Milk is an important food component in human diet and is a target for fraud, including many unsafe practices. For example, the unscrupulous adulteration of soymilk into bovine and goat milk, or bovine milk into goat milk in order to gain profit without declaration is a health risk as the adulterants source and sanitary history in unknown. A robust and fit-for-purpose technique is required to enforce market surveillance and hence protect consumers' health. Nuclear Magnetic Resonance (NMR) is a powerful technique for characterization of food products based on measuring the profile of metabolites...
October 28, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27771080/proteomics-method-to-quantify-the-percentage-of-cow-goat-and-sheep-milks-in-raw-materials-for-dairy-products
#20
Q Chen, X Ke, J S Zhang, S Y Lai, F Fang, W M Mo, Y P Ren
Fraud in milk and dairy products occurs when cow milk is added to sheep and goat milk for economic reasons. No reliable, selective, and sensitive method exists for quantifying the milk percentage of different species. This work reports the development and validation of a proteomics-based method for the qualitative detection and quantitative determination of cow, sheep, and goat milks in the raw materials used for dairy products. β-Lactoglobulin was selected as the protein marker because it is a major protein in milk and whey powder...
October 19, 2016: Journal of Dairy Science
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