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https://www.readbyqxmd.com/read/29028813/evaluation-of-shedding-tissue-burdens-and-humoral-immune-response-in-goats-after-experimental-challenge-with-the-virulent-brucella-melitensis-strain-16m-and-the-reduced-virulence-vaccine-strain-rev-1
#1
Jennifer L Higgins, Mercedes Gonzalez-Juarrero, Richard A Bowen
Brucella melitensis is the causative agent of brucellosis in small ruminants and is of considerable economic and public health importance in many countries worldwide. The control of disease in humans depends on the control of disease in livestock; however, few counties with endemic B. melitensis infection have been able to successfully eradicate this pathogen. This underscores the need for further research on the pathogenesis of both virulent and vaccine strains of B. melitensis in the small ruminant host. The aim of the present study was to characterize clinical effects, tissue colonization, shedding, and humoral immune response following B...
2017: PloS One
https://www.readbyqxmd.com/read/29026537/negative-effects-of-long-term-feeding-of-high-grain-diets-to-lactating-goats-on-milk-fat-production-and-composition-by-regulating-gene-expression-and-dna-methylation-in-the-mammary-gland
#2
Ping Tian, Yanwen Luo, Xian Li, Jing Tian, Shiyu Tao, Canfeng Hua, Yali Geng, Yingdong Ni, Ruqian Zhao
BACKGROUND: It is well known that feeding a high concentrate (HC) diet to lactating ruminants likely induces subacute ruminal acidosis (SARA) and leads to a decrease in milk fat production. However, the effects of feeding a HC diet for long periods on milk fatty acids composition and the mechanism behind the decline of milk fat still remains poorly understood. The aim of this study was to investigate the impact of feeding a HC diet to lactating dairy goats on milk fat yield and fatty acids composition with an emphasis on the mechanisms underlying the milk fat depression...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29019657/trans-10-cis-12-conjugated-linoleic-acid-affects-expression-of-lipogenic-genes-in-mammary-gland-of-lactating-dairy-goats
#3
Huaiping Shi, Tianying Zhang, Cong Li, Jianjue Wang, Jiangtao Huang, Zhongyang Li
The molecular mechanisms on milk fat depression (MFD) in response to trans-10, cis-12 conjugated linoleic acid (t10c12-CLA) supplementation in ruminants, were elucidated in this research with dairy goats. Thirty two-year-old Xinong Saanen dairy goats (40 ± 5 days in milk (DIM)) at peak lactation stage were assigned to a 3×3 Latin Square design (14-d treatment period followed with 14-d washout). Three CLA treatments included a) Control, fed the basal diet only without CLA supplementation; b) orally supplemented with 8 g/d of lipid-encapsulated CLA (low dose; CLA-1); and c) 16 g/d of lipid-encapsulated CLA (high dose; CLA-2)...
October 11, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29018987/seasonal-dynamics-of-endoparasitic-infections-at-an-organic-goat-farm-and-the-impact-of-detected-infections-on-milk-production
#4
Iveta A Kyriánová, Jaroslav Vadlejch, Oldřich Kopecký, Iva Langrová
This study evaluated patterns and species composition of parasitic infections detected over a 1-year period at an organic goat farm. As a result of coprological examination, the overall prevalence of observed strongylids (99%), coccidia of the genus Eimeria (98%), and Muellerius capillaris lungworms (93%) was calculated. The most prevalent strongylids recovered from incubated fecal samples were Haemonchus contortus (42%), genera Trichostrongylus (23%), Oesophagostomum columbianum (13%), and Teladorsagia circumcincta (11%)...
October 10, 2017: Parasitology Research
https://www.readbyqxmd.com/read/29016691/generation-of-beta-lactoglobulin-knock-out-goats-using-crispr-cas9
#5
Wenjun Zhou, Yongjie Wan, Rihong Guo, Mingtian Deng, Kaiping Deng, Zhen Wang, Yanli Zhang, Feng Wang
Goat's milk, considered a substitute for cow's milk, has a high nutritional value. However, goat's milk contains various allergens, predominantly β-lactoglobulin (BLG). In this study, we employed the CRISPR/Cas9 system to target the BLG locus in goat fibroblasts for sgRNA optimization and generate BLG knock-out goats through co-injection of Cas9 mRNA and small guide RNAs (sgRNAs) into goat embryos at the one-cell stage. We firstly tested sgRNA editing efficiencies in goat fibroblast cells, and approximately 8...
2017: PloS One
https://www.readbyqxmd.com/read/28988560/effects-of-replacement-of-moringa-oleifera-for-berseem-clover-in-the-diets-of-nubian-goats-on-feed-utilisation-and-milk-yield-composition-and-fatty-acid-profile
#6
A E Kholif, G A Gouda, O A Olafadehan, M M Abdo
Replacement of conventional feedstuffs with cheap non-conventional ingredients may improve livestock performance and the quality of their products, particularly milk. The study considered the effects of Moringa oleifera (MO) foliage in replacement of berseem clover (BC) on feed utilisation and lactational performance in Nubian goats. A total of 16 lactating Nubian does, weighing 36.2±0.8 kg, were randomly assigned to four experimental treatments containing 0, 125, 250 and 375 g of MO per kg diet to replace 0 (M0), 25 (M25), 50 (M50) and 75% (M75) of BC (on dry matter (DM) basis) in a quadruplicated 4×4 Latin square design...
October 9, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28987867/neuroprotective-effect-of-a-new-variant-of-epo-nonhematopoietic-against-oxidative-stress
#7
C Castillo, S Zaror, M Gonzalez, A Hidalgo, C F Burgos, O I Cabezas, F Hugues, S P Jiménez, E González-Horta, I González-Chavarría, J Gavilán, R Montesino, O Sánchez, Manuela G Lopez, J Fuentealba, J R Toledo
Human erythropoietin is mainly recognized for its hematopoietic function; however, by binding to its receptor (EpoR), it can activate different signaling pathways as STAT, PI3K, MAPK and RAS to increase cellular differentiation or provide neuroprotective effects, among others. A recombinant human erythropoietin variant with low glycosylation and without hematopoietic effect (EpoL) was purified from skimmed goat milk. Recombinant human erythropoietin (Epo) was obtained from CHO cell line and used as control to compare EpoL effects...
September 21, 2017: Redox Biology
https://www.readbyqxmd.com/read/28987350/identification-of-cow-milk-in-goat-milk-by-nonlinear-chemical-fingerprint-technique
#8
Yong-Jie Ma, Wen-Bin Dong, Cheng Fan, Er-Dan Wang
The objective of this paper was to develop a nonlinear chemical fingerprint technique for identifying and detecting adulteration of goat milk with cow milk. In this study, by taking the Belousov-Zhabotinsky oscillatory chemical reaction using acetone and substrates in goat milk or cow milk as main dissipative substances, when the same dosage of goat milk and cow milk was introduced to the "H(+) + Mn(2+) + BrO3(-) + acetone" oscillating system respectively, nonlinear chemical fingerprints were obtained for goat milk and cow milk from the same origin...
October 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28968291/protein-digestion-and-quality-of-goat-and-cow-milk-infant-formula-and-human-milk-under-simulated-infant-conditions
#9
Annet Maathuis, Robert Havenaar, Tao He, Susann Bellmann
OBJECTIVE: To determine the kinetics of true ileal protein digestion and digestible indispensable amino acid score (DIAAS) of a goat milk based infant formula (GIF), a cow milk based infant formula (CIF) and human milk (HM). METHODS: The GIF, CIF and HM were investigated in an in vitro gastrointestinal model simulating infant conditions. Digested compounds were dialyzed from the intestinal compartment as bioaccessible fraction. Dialysate was collected in 15-60 min periods for 4 h...
September 29, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28963210/draft-genome-sequences-of-historical-strains-of-coxiella-burnetii-isolated-from-cow-s-milk-and-a-goat-placenta
#10
Paul A Beare, Brendan M Jeffrey, Craig A Martens, Talima Pearson, Robert A Heinzen
Here, we report draft genome sequences of historical strains of Coxiella burnetii derived from cow's milk and the placenta of a goat that had aborted. The California and Ohio milk strains display a different sequence type than do contemporary milk strains.
September 28, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28959346/differential-expression-of-the-alpha-s1-casein-and-beta-lactoglobulin-genes-in-different-physiological-stages-of-the-adani-goats-mammary-glands
#11
Salim Morammazi, Ali Akbar Masoudi, Rasoul Vaez Torshizi, Abbas Pakdel
BACKGROUND: Milk proteins genes have been the focus of the researches as the candidate target genes that play a decisive role when animal breeding is desired. OBJECTIVES: In the present study, the transcriptional levels of Beta-lactoglobulin (BLG) and Alpha S1 casein (CSN1S1) genes were investigated during prenatal, milking and drying times in mammary glands of the Adani goats which showed high and low breeding values. MATERIALS AND METHODS: The breeding values of the animals were estimated first by applying multi-trait random regression model...
December 2016: Iranian journal of biotechnology
https://www.readbyqxmd.com/read/28954839/young-pigs-consuming-lysozyme-transgenic-goat-milk-are-protected-from-clinical-symptoms-of-enterotoxigenic-escherichia-coli-infection
#12
Lydia C Garas, Caitlin A Cooper, Matthew W Dawson, Jane-Ling Wang, James D Murray, Elizabeth A Maga
Background: Diarrheal diseases in infancy and childhood are responsible for substantial morbidity and mortality in developing nations. Lysozyme, an antimicrobial component of human milk, is thought to play a role in establishing a healthy intestinal microbiota and immune system. Consumption of breast milk has been shown to prevent intestinal infections and is a recommended treatment for infants with diarrhea.Objective: This study aimed to examine the ability of lysozyme-rich goat milk to prevent intestinal infection...
September 27, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28954470/first-report-of-a-i-staphylococcus-caprae-i-isolated-from-middle-ear-fluid-of-an-infant-with-recurrent-acute-otitis-media
#13
Elżbieta Mazur, Piotr Żychowski, Marek Juda, Izabela Korona-Głowniak, Grażyna Niedzielska, Anna Malm, Maria Kozioł-Montewka
<i>Staphylococcus caprae</i> was originally isolated from goat milk. This uncommon coagulase-negative staphylococcus, usually associated with animals, has only infrequently been detected in human clinical specimens. Its association with acute otitis media has not been demonstrated so far. The study reports the first isolation of <i>S. caprae</i> from the middle ear fluid of a 12-month-old infant with recurrent, bilateral acute otitis media. Biochemical traits and susceptibility pattern of the isolated strain are also presented...
September 21, 2017: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/28949782/divergent-anticancer-activity-of-free-and-formulated-camel-milk-%C3%AE-lactalbumin
#14
Vladimir N Uversky, Esmail M El-Fakharany, Marwa M Abu-Serie, Hussein A Almehdar, Elrashdy M Redwan
Alpha-lactalbumin (α-LA), a small milk calcium-binding globular protein, is known to possess noticeable anticancer activity, which is determined by the ability of this protein to form complexes with oleic acid (OA). To date, in addition to human and bovine α-LA, the ability to form such anti-tumor complexes with OA was described for goat and camel α-LA. Although the mechanisms of the anticancer activity of human and bovine α-LA are already well-studied, little is currently known about the anticancer action of this camel protein...
September 26, 2017: Cancer Investigation
https://www.readbyqxmd.com/read/28941817/evolution-of-free-amino-acids-during-ripening-of-caciocavallo-cheeses-made-with-different-milks
#15
Serena Niro, Mariantonietta Succi, Patrizio Tremonte, Elena Sorrentino, Raffaele Coppola, Gianfranco Panfili, Alessandra Fratianni
The evolution of free amino acids (FAA) in Caciocavallo cheeses, made with cow milk (CC) and cow milk mixed with ewe (CE) and goat (CG) milk, was studied throughout ripening. In all Caciocavallo cheeses produced, the total free amino acid (TFAA) content increased during ripening. In general, the highest TFAA content was found in cow cheeses, and the lowest in CG cheeses, whereas CE cheeses ranged over an intermediate level. In all the analyzed samples, during ripening, the content of the individual FAA increased with the exception of arginine...
September 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28934997/teatcups-with-automatic-valves-in-machine-milking-of-goats
#16
Alberto Manzur, José Ramón Díaz, Nemesio Fernández, Sebastià Balasch, Cristòfol Peris
This Research Paper addresses the hypothesis that using teatcups with automatic valves, without cutting off the vacuum prior to cluster removal, could increases the risk of mastitis and affect other milking variables on goats. A first trial used 46 intramammary infection (IMI)-free goats that had been milked with normal teatcups (without automatic valves) during a pre-experimental period of 8 ± 2 d postpartum. These animals were divided into two groups (n = 23), randomly assigning each group to teatcups with automatic valves (teatcups A) or without automatic valves (teatcups B) for a 20-week experimental period...
September 22, 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/28924723/sixteen-chinese-pediatric-brucellosis-patients-onset-of-fever-in-non-epidemic-areas-and-8-developed-with-osteoarticular-involvement
#17
Xinning Wang, Yuchun Yan, Fengqi Wu, Gaixiu Su, Shengnan Li, Xinyu Yuan, Jianming Lai, Zhixuan Zhou
The purpose of this study is to summarize the manifestations, diagnosis, differential diagnosis, and treatment of childhood brucellosis in non-epidemic areas of China. A retrospective review of 16 admitted children patients with brucella's disease who were diagnosed of brucellosis during the period from 2011 to 2016 was performed. Diagnostic criteria, clinical presentations, and outcomes were recorded. The most common symptom was fever. Osteoarticular involvement was found in 50% of the patients. They were infected by contacting with infected animals or consuming of unpasteurized milk or meat of sheep or goats, also...
September 19, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/28922262/sugar-in-infants-children-and-adolescents-a-position-paper-of-the-european-society-for-paediatric-gastroenterology-hepatology-and-nutrition-committee-on-nutrition
#18
Nataša Fidler Mis, Christian Braegger, Jiri Bronsky, Cristina Campoy, Magnus Domellöf, Nicholas D Embleton, Iva Hojsak, Jessie Hulst, Flavia Indrio, Alexandre Lapillonne, Walter Mihatsch, Christian Molgaard, Rakesh Vora, Mary Fewtrell
The consumption of sugars, particularly sugar-sweetened beverages (SSBs; beverages or drinks that contain added caloric sweeteners (i.e. sucrose, high-fructose corn syrup, fruit-juice concentrates), in European children and adolescents exceeds current recommendations. This is of concern because there is no nutritional requirement for free sugars, and infants have an innate preference for sweet taste, which may be modified and reinforced by pre- and postnatal exposures. Sugar containing beverages/free sugars increase the risk for overweight/obesity and dental caries, can result in poor nutrient supply and reduced dietary diversity and may be associated with increased risk of type 2 diabetes mellitus, cardiovascular risk, and other health effects...
September 16, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28920416/effects-of-aflatoxin-b1-combined-with-ochratoxin-a-and-or-zearalenone-on-metabolism-immune-function-and-antioxidant-status-in-lactating-dairy-goats
#19
Shuai Huang, Nan Zheng, CaiYun Fan, Ming Cheng, Shang Wang, Adil Jabar, JiaQi Wang, JianBo Cheng
Objective: This experiment was conducted to investigate the effects of aflatoxin B1 (AFB1) alone or mixed with ochratoxin A (OTA) and/or zearalenone (ZEA) on the metabolism, immune function, and antioxidant status of dairy goats. Methods: Fifty lactating Laoshan dairy goats were randomly assigned to one of five treatment groups (n = 10) for 14 days. Goats were fed no additive (control) or administered with 50 μg AFB1/kg DM (AFB1), 50 μg AFB1/kg DM + 100 μg OTA/kg DM (AFB1+OTA), 50 μg AFB1/kg DM + 500 μg ZEA/kg DM (AFB1+ZEA), or 50 μg AFB1/kg DM + 100 μg OTA/kg DM + 500 μg ZEA/kg DM (AFB1+OTA+ZEA)...
September 18, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28918468/constraints-affecting-dairy-goats-milk-production-in-kenya
#20
C M Mbindyo, C G Gitao, S G Peter
In Kenya, the population of dairy goats is about 200,000 and 80% of these are reared in Mount Kenya region. They provide a quick source of milk for consumption or sale, which has an immense value especially to poor households. The small land sizes required for their rearing are especially useful in these highly populated areas. Although much research has been done on problems faced by dairy cattle farmers, limited information is available on problems faced by dairy goat farmers. Therefore, the objective of this study was to determine the constraints affecting dairy goat production in Mount Kenya region...
September 16, 2017: Tropical Animal Health and Production
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