Shuo Zhang, Tinghe Fang, Yexuan He, Weichen Feng, Zhuoyang Yu, Yaoyao Zheng, Chi Zhang, Shuai Hu, Zhuojun Liu, Jia Liu, Jian Yu, Han Zhang, Anbang He, Yanqing Gong, Zhisong He, Kaiwei Yang, Zhijun Xi, Wei Yu, Liqun Zhou, Lin Yao, Shuhua Yue
BACKGROUND: Cholesteryl ester (CE) accumulation in intracellular lipid droplets (LDs) is an essential signature of clear cell renal cell carcinoma (ccRCC), but its molecular mechanism and pathological significance remain elusive. METHODS: Enabled by the label-free Raman spectromicroscopy, which integrated stimulated Raman scattering microscopy with confocal Raman spectroscopy on the same platform, we quantitatively analyzed LD distribution and composition at the single cell level in intact ccRCC cell and tissue specimens in situ without any processing or exogenous labeling...
April 1, 2024: EBioMedicine