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Immunohistochemistry for pancreatic neoplasms

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https://www.readbyqxmd.com/read/28821198/cd200-expression-in-neuroendocrine-neoplasms
#1
Jason E Love, Kimberly Thompson, Mark R Kilgore, Maria Westerhoff, Claire E Murphy, Antonios Papanicolau-Sengos, Kinsey A McCormick, Veena Shankaran, Natalie Vandeven, Faith Miller, Astrid Blom, Paul T Nghiem, Steven J Kussick
Objectives: CD200 expression has been well studied in hematopoietic malignancies; however, CD200 expression has not been well-characterized in neuroendocrine neoplasms. We examined CD200 expression in 391 neuroendocrine neoplasms from various anatomic sites. Methods: Tissue blocks containing pulmonary small cell carcinoma, pulmonary carcinoid, large cell neuroendocrine carcinoma, pancreatic neuroendocrine tumor, gastrointestinal carcinoid, and Merkel cell carcinoma were evaluated for CD200 expression by immunohistochemistry...
September 1, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28807337/evaluation-of-cd43-expression-in-non-hematopoietic-malignancies
#2
Bjorn H Batdorf, Steven H Kroft, Paul R Hosking, Alexandra M Harrington, Alexander C Mackinnon, Horatiu Olteanu
OBJECTIVES: CD43 is normally expressed only on the surface of leukocytes, and is considered a sensitive and specific marker for hematologic malignancies. As such, it may have diagnostic utility in confirming hematolymphoid lineage in cases that are negative for CD45. Aberrant CD43 expression has been described in non-hematopoietic tumors, although literature data on this topic is variable and sometimes contradictory. To clarify and expand on existing literature findings, we evaluated CD43 expression by immunohistochemistry (IHC) in a large cohort (307) of non-hematopoietic neoplasms, including poorly differentiated malignancies...
August 2017: Annals of Diagnostic Pathology
https://www.readbyqxmd.com/read/28802095/the-diagnostic-utility-of-merkel-cell-polyomavirus-immunohistochemistry-in-a-fine-needle-aspirate-of-metastatic-merkel-cell-carcinoma-of-unknown-primary-to-the-pancreas
#3
Long Li, Kyle Molberg, Naga Cheedella, Joel Thibodeaux, Stacy Hinson, Elena Lucas
Merkel cell carcinoma (MCC) is an aggressive skin tumor with a high tendency for metastases. We report a case of MCC initially presenting as axillary and pancreatic metastases. A 33-year-old HIV-positive Hispanic male presented with a history of a rapidly growing axillary mass. A needle core biopsy demonstrated an epithelioid neoplasm composed of small to medium-sized cells with high nuclear-cytoplasmic ratio, nuclear molding, and frequent mitotic figures. A subsequent PET scan revealed a 1.5 cm FDG avid mass in the pancreas...
August 12, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/28776576/gnas-mutations-in-primary-mucinous-and-non-mucinous-lung-adenocarcinomas
#4
Lauren L Ritterhouse, Marina Vivero, Mari Mino-Kenudson, Lynette M Sholl, A John Iafrate, Valentina Nardi, Fei Dong
GNAS mutations have been described in mucinous and non-mucinous epithelial neoplasms of the appendix, pancreas, and colon, with hotspot GNAS mutations found in up to two-thirds of pancreatic intraductal papillary mucinous neoplasms. Additionally, many GNAS-mutated tumors have concurrent mutations in the Ras/Raf pathway. The clinicopathologic features of GNAS-mutated lung carcinomas, however, have not yet been characterized. Primary lung carcinomas from Brigham and Women's Hospital (n=1282) or Massachusetts General Hospital (n=1070) were genotyped on a targeted massively parallel sequencing panel of oncogenes and tumor suppressor genes including GNAS...
August 4, 2017: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/28752676/fine-needle-aspiration-findings-of-a-rare-hematopoietic-neoplasm-presenting-as-obstructive-jaundice
#5
Jessica Tracht, Ali M Ahmed, Frida Rosenblum Donath
A 51-year-old female who presented with obstructive jaundice was found to have masses in the pancreatic head and tail as well as suspicious liver and periaortic masses on imaging. Aspiration cytology of the pancreatic tail mass showed abundant large single cells with vacuolated eosinophilic cytoplasm, marked nuclear pleomorphism, large bizarre irregular nuclei, binucleation, and prominent nucleoli. Numerous cells also showed intracytoplasmic black to brown pigmentation. A cell block was obtained and extensive immunohistochemical staining was performed...
July 27, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/28599853/post-obstructive-cyst-formation-in-pancreas-and-cystic-acinar-transformation-are-they-same
#6
Xuchen Zhang, Hongfa Zhu, Xiu Yang, Volkan N Adsay, Dhanpat Jain
Pancreatic "acinar cell cystadenoma" (PACA) is a rare benign pancreatic cystic lesion showing acinar cell differentiation. The neoplastic nature of PACA has been questioned and its exact pathogenesis remains unclear. To investigate that acinar cell differentiation is a non-specific metaplastic phenomenon that can occur in pancreatic ductal system, especially when chronically inflamed and dilated, and doesn't necessarily imply an acinar cell neoplasm, we retrospectively analyzed cases diagnosed as PACA and cases with post-obstructive cystic dilatation of pancreatic ducts for acinar cell differentiation using immunohistochemistry for trypsin...
August 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28435405/establishment-and-characterization-of-6-novel-patient-derived-primary-pancreatic-ductal-adenocarcinoma-cell-lines-from-korean-pancreatic-cancer-patients
#7
Mi-Ju Kim, Min-Sun Kim, Sung Joo Kim, Soyeon An, Jin Park, Hosub Park, Jae Hoon Lee, Ki-Byung Song, Dae Wook Hwang, Suhwan Chang, Kyu-Pyo Kim, Seong-Yun Jeong, Song Cheol Kim, Seung-Mo Hong
BACKGROUND: Pancreatic ductal adenocarcinomas are among the most malignant neoplasms and have very poor prognosis. Our understanding of various cancers has recently improved the survival of patients with cancer, except for pancreatic cancers. Establishment of primary cancer cell lines of pancreatic ductal adenocarcinomas will be useful for understanding the molecular mechanisms of this disease. METHODS: Eighty-one surgically resected pancreatic ductal adenocarcinomas were collected...
2017: Cancer Cell International
https://www.readbyqxmd.com/read/28316222/-the-clinical-characteristics-surgical-procedures-and-prognosis-of-solid-pseudopapillary-tumor-of-the-pancreas
#8
H Yang, N Li, W Y Deng, S X Luo
Objective: To explore the clinical characteristics, surgical procedures and prognosis of solid pseudopapillary tumor of the pancreas(SPTP). Methods: The clinical and follow-up data of 55 cases with SPTP in Henan Tumor Hospital from June 2005 to April 2015 were retrospectively analyzed. Results: There were 55 SPTP cases, including 7 males and 48 females. The age ranged from 16 to 76 (median, 33). Clinical presentations of SPTP were not specific. The mean size of the tumor was 7.6 cm (range from 2 to 25cm). Pancreatic head and tail were the most common locations of SPTP...
March 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/27864124/cd133-expression-in-well-differentiated-pancreatic-neuroendocrine-tumors-a-potential-predictor-of-progressive-clinical-courses
#9
Yasuhiro Sakai, Seung-Mo Hong, Soyeon An, Joo Young Kim, Denis Corbeil, Jana Karbanová, Kyoko Otani, Kohei Fujikura, Ki-Byung Song, Song Cheol Kim, Masayuki Akita, Yoshihide Nanno, Hirochika Toyama, Takumi Fukumoto, Yonson Ku, Takanori Hirose, Tomoo Itoh, Yoh Zen
The present study aimed to elucidate whether the stemness molecule, CD133, is expressed in well-differentiated pancreatic neuroendocrine tumors (PanNETs; WHO grades 1 and 2) and establish its clinical relevance using two separate cohorts. In the first series (n=178) in which tissue microarrays were available, immunohistochemistry revealed that CD133 was expressed in 14 cases (8%). CD133+ PanNETs had higher TNM stages (P<.01), more frequent lymphovascular invasion (P=.01), and higher recurrence rates (P=...
November 15, 2016: Human Pathology
https://www.readbyqxmd.com/read/27756613/pancreatic-juice-cytology-with-immunohistochemistry-to-detect-malignancy-and-histologic-subtypes-in-patients-with-branch-duct-type-intraductal-papillary-mucinous-neoplasms-of-the-pancreas
#10
Shinsuke Koshita, Yutaka Noda, Kei Ito, Yoshihide Kanno, Takahisa Ogawa, Kaori Masu, Yoshiharu Masaki, Jun Horaguchi, Masaya Oikawa, Takashi Tsuchiya, Takashi Sawai, Miwa Uzuki, Fumiyoshi Fujishima
BACKGROUND AND AIMS: The aim of this study was to elucidate the efficacy of pancreatic juice cytology with the cell-block method (CB-PJC) for the determination of surgery in patients with branch duct intraductal papillary mucinous neoplasm (BD-IPMN). METHODS: In 138 patients with BD-IPMN from whom pancreatic juice was collected under ERCP for CB-PJC, we retrospectively evaluated the following: (1) the rate of successfully evaluated CB-PJC; (2) the ability of CB-PJC to diagnose malignancy and to identify pathologic subtypes in resected BD-IPMNs; (3) the rate of development into invasive cancer and progression of BD-IPMNs in patients with BD-IPMNs diagnosed as benignancy by CB-PJC; and (4) post-ERCP adverse events...
May 2017: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/27628324/mash1-is-highly-specific-for-neuroendocrine-carcinomas-an-immunohistochemical-evaluation-on-normal-and-various-neoplastic-tissues
#11
David Altree-Tacha, Jillian Tyrrell, Faqian Li
CONTEXT: -High-grade neuroendocrine carcinomas and carcinoids can arise in different sites such as lung, gastrointestinal tract, prostate, and skin. Classic neuroendocrine markers such as CD56, synaptophysin, and chromogranin cannot distinguish carcinoids from high-grade neuroendocrine carcinomas. Recently, mouse monoclonal mASH1 has been shown to help discriminate carcinoids from high-grade neuroendocrine carcinomas in various neoplastic sites. To date, there have been no comprehensive immunohistochemistry studies with mASH1 on nonneuroendocrine neoplasms...
February 2017: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/27567273/immunohistochemical-detection-of-pax8-and-napsin-a-in-canine-thyroid-tumours-comparison-with-thyroglobulin-calcitonin-and-thyroid-transcription-factor-1
#12
J A Ramos-Vara, C B Frank, D DuSold, M A Miller
Expression of thyroid transcription factor (TTF)-1 corroborates a thyroid origin of neoplasms. Thyroglobulin and calcitonin immunohistochemistry (IHC) can distinguish between a follicular and C-cell origin of thyroid tumours, respectively. Pax8 (expressed by normal canine thyroid follicular cells) and napsin A (expressed mainly by C-cells) labelling was compared with labelling for TTF-1, thyroglobulin and calcitonin in 114 canine proliferative thyroid lesions. All 81 follicular tumours expressed thyroglobulin and were negative for calcitonin; 79/81 (98%) of these tumours expressed TTF-1 and Pax8 and 60/81 (74%) expressed napsin A...
November 2016: Journal of Comparative Pathology
https://www.readbyqxmd.com/read/27523981/loss-of-trefoil-factor-2-from-pancreatic-duct-glands-promotes-formation-of-intraductal-papillary-mucinous-neoplasms-in-mice
#13
Junpei Yamaguchi, Mari Mino-Kenudson, Andrew S Liss, Sanjib Chowdhury, Timothy C Wang, Carlos Fernández-Del Castillo, Keith D Lillemoe, Andrew L Warshaw, Sarah P Thayer
BACKGROUND & AIMS: Little is known about the origin of pancreatic intraductal papillary mucinous neoplasms (IPMN). Pancreatic duct glands (PDGs) are gland-like outpouches budding off the main pancreatic ducts that function as a progenitor niche for the ductal epithelium; they express gastric mucins and have characteristics of side-branch IPMNs. We investigated whether PDGs are a precursor compartment for IPMNs and the role of Trefoil factor family 2 (TFF2)-a protein expressed by PDGs and the gastric mucosa that are involved in epithelial repair and tumor suppression...
December 2016: Gastroenterology
https://www.readbyqxmd.com/read/27459312/intraductal-tubulopapillary-neoplasm-of-the-pancreas-masquerading-as-pancreatic-neuroendocrine-carcinoma-review-of-the-literature-with-a-case-report
#14
Deepika Savant, Lili Lee, Kasturi Das
BACKGROUND: Intraductal tubulopapillary neoplasm of the pancreas (ITPN) is a rare tumor which was first described in 2009. We report a case with cytologic and histologic findings and discuss the pitfalls in diagnosing this entity on cytology. CASE: An 82-year-old female presented with a pancreatic body mass measuring 3.3 cm. Endoscopic ultrasound-guided fine-needle aspiration showed cells in cohesive clusters with high-grade nuclear atypia. Immunohistochemistry (IHC) showed the neoplastic cells to be positive for CK19, CD56 and chromogranin (focal)...
2016: Acta Cytologica
https://www.readbyqxmd.com/read/27407094/alternative-lengthening-of-telomeres-and-loss-of-daxx-atrx-expression-predicts-metastatic-disease-and-poor-survival-in-patients-with-pancreatic-neuroendocrine-tumors
#15
Aatur D Singhi, Ta-Chiang Liu, Justin L Roncaioli, Dengfeng Cao, Herbert J Zeh, Amer H Zureikat, Allan Tsung, J Wallis Marsh, Kenneth K Lee, Melissa E Hogg, Nathan Bahary, Randall E Brand, Kevin M McGrath, Adam Slivka, Kristi L Cressman, Kimberly Fuhrer, Roderick J O'Sullivan
PURPOSE: Pancreatic neuroendocrine tumors (PanNET) are a heterogeneous group of neoplasms with increasing incidence and unpredictable behavior. Whole-exome sequencing has identified recurrent mutations in the genes DAXX and ATRX, which correlate with loss of protein expression and alternative lengthening of telomeres (ALT). Both ALT and DAXX/ATRX loss were initially reported to be associated with a favorable prognosis; however, recent studies suggest the contrary. Our aims were to assess the prevalence and prognostic significance of ALT and DAXX/ATRX in both primary and metastatic PanNETs...
July 12, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/27381533/differential-diagnosis-between-pancreatic-neuroendocrine-and-solid-pseudopapillary-neoplasms-on-endoscopic-ultrasound-guided-fine-needle-aspiration-an-immunohistochemical-study
#16
Emad M Raddaoui, Majid A Almadi, Abdulrahman M Aljebreen, Faisal A Alsaif, Ahlam A AlShedoukhy, Abed H Al-Lehibi, Khalid A Almohameed, Apostolos V Tsolakis, Mousa A AlAbbadi, Amna R Almutrafi
OBJECTIVES: To evaluate the role of applying a limited panel of immunohistochemical stains on the cellblock preparation from samples obtained by endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) in the aim of differentiating solid pseudopapillary neoplasms (SPNs) from neuroendocrine tumors (NETs).  METHODS: We retrospectively retrieved all the EUS-FNAs of the pancreas that have a diagnosis of NET or SPN that were performed at 2 tertiary care hospitals in Riyadh, Kingdom of Saudi Arabia from May 2004 to December 2014...
July 2016: Saudi Medical Journal
https://www.readbyqxmd.com/read/27212431/digestive-neuroendocrine-neoplasms-a-2016-overview
#17
REVIEW
Elettra Merola, Maria Rinzivillo, Noemi Cicchese, Gabriele Capurso, Francesco Panzuto, Gianfranco Delle Fave
Digestive neuroendocrine neoplasms (DNENs) have an incidence of 2.39 per 100,000 inhabitants per year, and a prevalence of 35 cases per 100,000; the gap between these rates is to be referred to the relatively long survival that characterizes the majority of these tumors, which can be thus considered as chronic oncological diseases. Up to 80% of patients are stage IV since the first diagnosis, presenting a 5-yr overall survival rate of 35%-55% and a twice higher mortality than limited disease. DNENs express somatostatin receptors in more than 80% of cases, detected through immunohistochemistry or functional imaging tests (FITs)...
August 2016: Digestive and Liver Disease
https://www.readbyqxmd.com/read/27198568/interobserver-variability-in-intraductal-papillary-mucinous-neoplasm-subtypes-and-application-of-their-mucin-immunoprofiles
#18
Heewon A Kwak, Xiuli Liu, Daniela S Allende, Rish K Pai, John Hart, Shu-Yuan Xiao
Intraductal papillary mucinous neoplasm is considered a precursor lesion to pancreatic adenocarcinoma. These are further classified into four histologic subtypes: gastric, intestinal, pancreatobiliary, and oncocytic. The first aim of this study was to assess the interobserver variability among five gastrointestinal pathologists in diagnosing intraductal papillary mucinous neoplasm subtypes by morphology alone. The second aim of the study was to compare intraductal papillary mucinous neoplasm subtypes, which received consensus diagnoses (≥80% agreement) with their respective mucin immunoprofiles (MUC1, MUC2, MUC5AC, MUC6, and CDX2)...
September 2016: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/27095449/expansion-of-epigenetic-alterations-in-efemp1-promoter-predicts-malignant-formation-in-pancreatobiliary-intraductal-papillary-mucinous-neoplasms
#19
Kazuhiro Yoshida, Takeshi Nagasaka, Yuzo Umeda, Takehiro Tanaka, Keisuke Kimura, Fumitaka Taniguchi, Tomokazu Fuji, Kunitoshi Shigeyasu, Yoshiko Mori, Hiroyuki Yanai, Takahito Yagi, Ajay Goel, Toshiyoshi Fujiwara
PURPOSE: Although limited understanding exists for the presence of specific genetic mutations and aberrantly methylated genes in pancreatobiliary intraductal papillary mucinous neoplasms (IPMNs), the fundamental understanding of the dynamics of methylation expansion across CpG dinucleotides in specific gene promoters during carcinogenesis remains unexplored. Expansion of DNA methylation in some gene promoter regions, such as EFEMP1, one of the fibulin family, with tumor progression has been reported in several malignancies...
July 2016: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/27034817/non-pancreatic-retroperitoneal-mucinous-neoplasms-and-a-discussion-of-the-differential-diagnosis
#20
Summer E Blount, Camilla Cobb, Anwar Sultana Raza
Retroperitoneal mucinous neoplasms are common and have a wide range of differential diagnoses to consider. It is of utmost importance to maintain a multidisciplinary approach when evaluating these lesions. Clinical history, surgical impression and radiographic studies should be obtained and used in conjunction with morphology and immunohistochemistry to help guide the pathologist to the correct diagnosis. We recently encountered a case of a 51-year-old man who presented with an 11.5 cm left-sided retroperitoneal mucinous neoplasm...
April 2016: Journal of Gastrointestinal Oncology
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