C Coral Domínguez-Medina, Marisol Pérez-Toledo, Anna E Schager, Jennifer L Marshall, Charlotte N Cook, Saeeda Bobat, Hyea Hwang, Byeong Jae Chun, Erin Logan, Jack A Bryant, Will M Channell, Faye C Morris, Sian E Jossi, Areej Alshayea, Amanda E Rossiter, Paul A Barrow, William G Horsnell, Calman A MacLennan, Ian R Henderson, Jeremy H Lakey, James C Gumbart, Constantino López-Macías, Vassiliy N Bavro, Adam F Cunningham
Lipopolysaccharide (LPS) O-antigen (O-Ag) is known to limit antibody binding to surface antigens, although the relationship between antibody, O-Ag and other outer-membrane antigens is poorly understood. Here we report, immunization with the trimeric porin OmpD from Salmonella Typhimurium (STmOmpD) protects against infection. Atomistic molecular dynamics simulations indicate this is because OmpD trimers generate footprints within the O-Ag layer sufficiently sized for a single IgG Fab to access. While STmOmpD differs from its orthologue in S...
February 12, 2020: Nature Communications