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https://www.readbyqxmd.com/read/28738416/conditionally-immortal-slc4a11-mouse-corneal-endothelial-cell-line-recapitulates-disrupted-glutaminolysis-seen-in-slc4a11-mouse-model
#1
Wenlin Zhang, Diego G Ogando, Edward T Kim, Moon-Jung Choi, Hongde Li, Jason M Tenessen, Joseph A Bonanno
Purpose: To establish conditionally immortal mouse corneal endothelial cell lines with genetically matched Slc4a11+/+ and Slc4a11-/- mice as a model for investigating pathology and therapies for SLC4A11 associated congenital hereditary endothelial dystrophy (CHED) and Fuchs' endothelial corneal dystrophy. Methods: We intercrossed H-2Kb-tsA58 mice (Immortomouse) expressing an IFN-γ dependent and temperature-sensitive mutant of the SV40 large T antigen (tsTAg) with Slc4a11+/+ and Slc4a11-/- C57BL/6 mice...
July 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28738195/proteomic-and-genetics-insights-on-the-response-of-the-bacteriocinogenic-lactobacillus-sakei-crl1862-during-biofilm-formation-on-stainless-steel-surface-at-10%C3%A2-c
#2
Mariana Pérez-Ibarreche, Lucía M Mendoza, Graciela Vignolo, Silvina Fadda
Some lactic acid bacteria have the ability to form biofilms on food-industry surfaces and this property could be used to control food pathogens colonization. Lactobacillus sakei CR1862 was selected considering its bacteriocinogenic nature and ability to adhere to abiotic surfaces at low temperatures. In this study, the proteome of L. sakei CRL1862 grown either under biofilm on stainless steel surface and planktonic modes of growth at 10°C, was investigated. Using two-dimensional gel electrophoresis, 29 out of 43 statistically significant spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry...
July 4, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28738064/species-a-rotavirus-nsp3-acquires-its-translation-inhibitory-function-prior-to-stable-dimer-formation
#3
Hugo I Contreras-Treviño, Edgar Reyna-Rosas, Renato León-Rodríguez, Blanca H Ruiz-Ordaz, Tzvetanka D Dinkova, Ana M Cevallos, Luis Padilla-Noriega
Species A rotavirus non-structural protein 3 (NSP3) is a translational regulator that inhibits or, under some conditions, enhances host cell translation. NSP3 binds to the translation initiation factor eIF4G1 and evicts poly-(A) binding protein (PABP) from eIF4G1, thus inhibiting translation of polyadenylated mRNAs, presumably by disrupting the effect of PABP bound to their 3'-ends. NSP3 has a long coiled-coil region involved in dimerization that includes a chaperone Hsp90-binding domain (HS90BD). We aimed to study the role in NSP3 dimerization of a segment of the coiled-coil region adjoining the HS90BD...
2017: PloS One
https://www.readbyqxmd.com/read/28738017/distinct-oxysterol-requirements-for-positioning-na%C3%A3-ve-and-activated-dendritic-cells-in-the-spleen
#4
Erick Lu, Eric V Dang, Jeffrey G McDonald, Jason G Cyster
Correct positioning of dendritic cells (DCs) is critical for efficient pathogen encounter and antigen presentation. Epstein-Barr virus-induced gene 2 (EBI2) has been identified as a chemoattractant receptor required for naïve CD4(+)DCIR2(+) DC positioning in response to 7α,25-hydroxycholesterol (7α,25-HC). We now provide evidence that a second EBI2 ligand, 7α,27-HC, is involved in splenic DCIR2(+) DC positioning and homeostasis. Cyp27a1, the enzyme uniquely required for 7α,27-HC synthesis, is expressed by stromal cells in the region of naïve DC localization...
April 7, 2017: Science Immunology
https://www.readbyqxmd.com/read/28737897/biogenic-hydroxyapatite-a-new-material-for-the-preservation-and-restoration-of-the-built-environment
#5
Ronald Joseph Turner, Joanna C Renshaw, Andrea Hamilton
Ordinary Portland Cement (OPC) is by weight the world's most produced man-made material, and consequently is used in a variety of applications in environments ranging from buildings, to nuclear wasteforms, and within the human body. In this paper, we present for the first time the direct deposition of biogenic hydroxyapatite onto the surface of OPC, in a synergistic process which relies upon the unique properties of the cement substrate. The synthesised hydroxyapatite is very similar to that found in nature; having a crystallite size, iron and carbonate substitution, and a semi-crystalline structure which identify that this biogenic hydroxyapatite is akin to that found in natural bone and tooth enamel...
July 24, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28737895/cell-wall-remodeling-of-staphylococcus-aureus-in-live-c-elegans
#6
Sean E Pidgeon, Marcos M Pires
Peptidoglycan (PG) scaffolds are critical components of bacterial cell walls. They counter internal turgor pressure to prevent lysis and protect against external insults. It was recently discovered that various types of bacteria release large quantities of PG building blocks (D-amino acids) into their surrounding medium. Cultured bacteria were also found to incorporate D-amino acids (both natural and synthetic) from the medium directly into their PG scaffold. Together, these two processes may potentially function, in concert, to metabolically remodel PG in live host organisms...
July 24, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28737753/metabolic-control-of-the-scaffold-protein-tks5-in-tissue-invasive-proinflammatory-t-cells
#7
Yi Shen, Zhenke Wen, Yinyin Li, Eric L Matteson, Jison Hong, Jörg J Goronzy, Cornelia M Weyand
Pathogenic T cells in individuals with rheumatoid arthritis (RA) infiltrate non-lymphoid tissue sites, maneuver through extracellular matrix and form lasting inflammatory microstructures. Here we found that RA T cells abundantly express the podosome scaffolding protein TKS5, which enables them to form tissue-invasive membrane structures. TKS5 overexpression was regulated by the intracellular metabolic environment of RA T cells-specifically, by reduced glycolytic flux that led to deficiencies in ATP and pyruvate...
July 24, 2017: Nature Immunology
https://www.readbyqxmd.com/read/28737742/systemic-transport-of-trans-zeatin-and-its-precursor-have-differing-roles-in-arabidopsis-shoots
#8
Asami Osugi, Mikiko Kojima, Yumiko Takebayashi, Nanae Ueda, Takatoshi Kiba, Hitoshi Sakakibara
Organ-to-organ signal transmission is essential for higher organisms to ensure coordinated biological reactions during metabolism and morphogenesis. Similar to organs in animals, plant organs communicate by various signalling molecules. Among them, cytokinins, a class of phytohormones, play a key role as root-to-shoot long-distance signals, regulating various growth and developmental processes in shoots(1,2). Previous studies have proposed that trans-zeatin-riboside, a type of cytokinin precursor, is a major long-distance signalling form in xylem vessels and its action depends on metabolic conversion via the LONELY GUY enzyme in proximity to the site of action(3-5)...
July 24, 2017: Nature Plants
https://www.readbyqxmd.com/read/28737485/risk-taking-during-feeding-between-and-within-population-variation-and-repeatability-across-contexts-among-wild-zebrafish
#9
Tamal Roy, Rohitashva Shukla, Anuradha Bhat
Behavioral responses are shaped by a complex interplay of extrinsic factors, such as predation and flow conditions, and intrinsic properties such as metabolic state. We investigated variations in boldness among four populations of zebrafish collected from widely varying habitat conditions across India. We measured feeding latencies of fish in four contexts: in a novel environment; in an environment with refuge and predators; in the presence of a shoal; and in the presence of a shoal and predators. The population from a high-flow and high-predation habitat was significantly bolder than populations from low-predation and low-flow/stagnant habitats...
July 24, 2017: Zebrafish
https://www.readbyqxmd.com/read/28737297/correlates-of-frailty-phenotype-and-frailty-index-and-their-associations-with-clinical-outcomes
#10
G Guaraldi, A Malagoli, O Theou, T D Brothers, Lmk Wallace, R Torelli, C Mussini, S Sartini, S A Kirkland, K Rockwood
OBJECTIVES: Frailty is a predictor of adverse health outcomes and can be measured across the life course, including among people living with HIV. The purpose of this study was to examine two commonly used measures of frailty - the frailty index (FI) and frailty phenotype - to assess common characteristics and to describe associations with multimorbidity, falls, and disability in people aging with HIV. METHODS: This was a cross-sectional observational study including 482 consecutive HIV-infected patients (mean age 53...
July 24, 2017: HIV Medicine
https://www.readbyqxmd.com/read/28737202/-euglycemic-ketosis-in-an-adolescent-with-type-1-diabetes-on-insulin-and-dapaglifozin-case-report
#11
Marcia Pereyra A, Cristhian Ramírez, Rossana Román
Dapagliflozin, an insulin-independent sodium-glucose cotransporter 2 inhibitor (SGLT2-I) induces glycosuria and reduces hyperglycemia in adults with type 2 diabetes. OBJECTIVE: To present an “euglycemic” diabetic ketosis in an adolescent with type 1 diabetes (T1D) receiving dapagliflozin, to alert about the risk of a drug not approved in children nor in T1D. CASE REPORT: A 17 years old adolescent with T1D during 9 years, was started on dapagliflozin 10 mg / day to reduce insulin dose and weight...
June 2017: Revista Chilena de Pediatría
https://www.readbyqxmd.com/read/28737010/carbonate-ooids-of-the-mesoarchaean-pongola-supergroup-south-africa
#12
M Siahi, A Hofmann, S Master, C W Mueller, A Gerdes
Ooids from the Mesoarchaean Chobeni Formation, Pongola Supergroup, KwaZulu-Natal, South Africa are older than any ooids reported to date. They are made of dolomite and ankerite and show concentric, radial-concentric, micritic, and radial fabrics. Radial ooids are interpreted to have originated from high-Mg-calcite and probably formed by microbial activity in a low-energy regime, while concentric ooids had an aragonite precursor and formed biotically under agitated/high-energy environmental conditions. Micritic ooids formed via the recrystallization of concentric ooids...
July 24, 2017: Geobiology
https://www.readbyqxmd.com/read/28736925/engineering-a-bzd-cassette-for-the-anaerobic-bioconversion-of-aromatic-compounds
#13
María Teresa Zamarro, María J L Barragán, Manuel Carmona, José Luis García, Eduardo Díaz
Microorganisms able to degrade aromatic contaminants constitute potential valuable biocatalysts to deal with a significant reusable carbon fraction suitable for eco-efficient valorization processes. Metabolic engineering of anaerobic pathways for degradation and recycling of aromatic compounds is an almost unexplored field. In this work, we present the construction of a functional bzd cassette encoding the benzoyl-CoA central pathway for the anaerobic degradation of benzoate. The bzd cassette has been used to expand the ability of some denitrifying bacteria to use benzoate as sole carbon source under anaerobic conditions, and it paves the way for future pathway engineering of efficient anaerobic biodegraders of aromatic compounds whose degradation generates benzoyl-CoA as central intermediate...
July 24, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28736440/repin1-deficiency-in-adipose-tissue-improves-whole-body-insulin-sensitivity-and-lipid-metabolism
#14
N Hesselbarth, A Kunath, M Kern, M Gericke, N Mejhert, M Rydén, M Stumvoll, M Blüher, N Klöting
BACKGROUND/OBJECTIVES: Replication initiator 1 (Repin1) gene encodes for a zinc-finger protein and has been implicated in the regulation of adipocyte cell size and glucose transport in vitro. Here, we investigate the consequences of reduced adipose tissue (AT) Repin1 expression in vivo. SUBJECTS/METHODS: We have inactivated the Repin1 gene in adipose tissue (iARep(-/-)) at an age of four weeks using tamoxifen-inducible gene targeting strategies on the background of C57BL/6NTac mice...
July 24, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/28736384/-revisional-surgery-for-nonstandard-gastric-bypass-a-case-report
#15
Yu Gao, Zhi-Gang Ke, Jian-Guo Liu, Wei-Dong Tong
Bariatric surgery is the most effective treatment for obesity with or without type 2 diabetes mellitus and its complications. With the growing incidence of type 2 diabetes mellitus, the number of hospitals performing bariatric surgery and surgical cases increase substantially. The problems in operation standardization, quality control of operation and postoperative management of the patients cause the failure in weight loss, hypoglycemia and severe complications, and revisional surgery is often required to correct the condition...
July 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28736128/the-effect-of-pro-nanolipospheres-pnl-formulation-containing-natural-absorption-enhancers-on-the-oral-bioavailability-of-delta-9-tetrahydrocannabinol-thc-and-cannabidiol-cbd-in-a-rat-model
#16
Irina Cherniakov, Dvora Izgelov, Abraham J Domb, Amnon Hoffman
The lipophilic phytocannabinoids cannabidiol (CBD) and Δ(9)-tetrahydrocannabinol (THC) show therapeutic efficacy in various medical conditions. Both molecules are poorly water soluble and subjected to extensive first pass metabolism in the gastrointestinal tract, leading to a limited oral bioavailability of approximately 9%. We have developed an advanced lipid based Self-Emulsifying Drug Delivery System termed Advanced Pro-NanoLiposphere (PNL) pre-concentrate. The PNL is composed of lipid and emulsifying excipients of GRAS status and are known to increase solubility and reduce Phase I metabolism of lipophilic active compounds...
July 20, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28735916/morbidity-and-mortality-of-middle-aged-and-elderly-narcoleptics
#17
Poul Jennum, Eva Wiberg Thorstensen, Line Pickering, Rikke Ibsen, Jakob Kjellberg
OBJECTIVES: The objective of the study was to evaluate the morbidities and mortality in a national group of middle-aged and elderly narcolepsy patients before and after the first diagnosis of the condition. METHODS: From the Danish National Patient Registry (NPR), 1174 patients (45.1% males) aged 20-59 years and 339 patients (44.8% males) aged 60+ who received a diagnosis of narcolepsy between 1998 and 2014 were compared, respectively, with 4716 and 1353 control citizens matched for age, gender and geography, who were randomly chosen from the Danish Civil Registration System Statistics...
August 2017: Sleep Medicine
https://www.readbyqxmd.com/read/28735646/hyperhomocysteinemia-and-age-related-macular-degeneration-role-of-inflammatory-mediators-and-pyroptosis-a-proposal
#18
Mahavir Singh, Suresh C Tyagi
Age-related macular degeneration (AMD) and pyroptosis cause irreversible vascular changes in the eyes leading to central vision loss in patients. It is the most common eye disease affecting millions of people aged 50years or older, and is slowly becoming a major health problem worldwide. The disease mainly affects macula lutea, an oval-shaped pigmented area surrounding fovea near the center of retina, a region responsible for visual acuity. It is fairly a complex disease as genetics of patients, environmental triggers as well as risk factors such as age, family history of CVDs, diabetes, gender, obesity, race, hyperopia, iris color, smoking, diabetes, exposure to sun light and pyroptosis have all been clubbed together as probable causes of macular degeneration...
August 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28735572/early-nutritional-intervention-can-improve-utilisation-of-vegetable-based-diets-in-diploid-and-triploid-atlantic-salmon-salmo-salar-l
#19
Michael Clarkson, Herve Migaud, Christoforos Metochis, Luisa M Vera, Daniel Leeming, Douglas R Tocher, John F Taylor
The present study investigated nutritional programming in Atlantic salmon to improve utilisation of a vegetable-based diet. At first exogenous feeding, fry were fed either a marine-based diet (Diet Mstimulus, 80% fishmeal (FM)/4% fish oil (FO)) or a vegetable-based diet (Diet Vstimulus, 10% FM/0% FO) for 3 weeks. Subsequently, all fish were then fed under the same conditions with a commercial, marine-based, diet for 15 weeks and thereafter challenged with a second V diet (Diet Vchallenge, 10% FM/0% FO) for 6 weeks...
July 24, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28735528/lipids-evergreen-autofluorescent-biomarkers-for-the-liver-functional-profiling
#20
Anna Cleta Croce, Giovanni Bottiroli
Depending on their chemical nature, lipids can be classified in two main categories: hydrophilic, greatly contributing to membrane composition and subcellular organelle compartmentalization, and hydrophobic, mostly triglycerides, greatly enrolled in the storage and production of energy. In both cases, some lipid molecules can be involved as signaling agents in the regulation of metabolism and protective or damaging pathways in responses to harmful stimuli. These events could affect in particular the liver, because of its central role in the maintenance of lipid homeostasis...
June 1, 2017: European Journal of Histochemistry: EJH
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