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https://www.readbyqxmd.com/read/29036290/comparative-genomics-of-apomictic-root-knot-nematodes-hybridization-ploidy-and-dynamic-genome-change
#1
Amir Szitenberg, Laura Salazar-Jaramillo, Vivian C Blok, Dominik R Laetsch, Soumi Joseph, Valerie M Williamson, Mark L Blaxter, David H Lunt
The Root-Knot Nematodes (RKN; genus Meloidogyne) are important plant parasites causing substantial agricultural losses. The Meloidogyne incognita group (MIG) of species, most of which are obligatory apomicts (mitotic parthenogens), are extremely polyphagous and important problems for global agriculture. While understanding the genomic basis for their variable success on different crops could benefit future agriculture, analyses of their genomes are challenging due to complex evolutionary histories that may incorporate hybridization, ploidy changes, and chromosomal fragmentation...
September 25, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29028986/a-new-haplotype-block-detection-method-for-dense-genome-sequencing-data-based-on-interval-graph-modeling-of-clusters-of-highly-correlated-snps
#2
Sun Ah Kim, Chang-Sung Cho, Suh-Ryung Kim, Shelley B Bull, Yun Joo Yoo
Motivation: Linkage disequilibrium (LD) block construction is required for research in population genetics and genetic epidemiology, including specification of sets of single nucleotide polymorphisms (SNPs) for analysis of multi-SNP based association and identification of haplotype blocks in high density sequencing data. Existing methods based on a narrow sense definition do not allow intermediate regions of low LD between strongly associated SNP pairs and tend to split high density SNP data into small blocks having high between-block correlation...
September 29, 2017: Bioinformatics
https://www.readbyqxmd.com/read/29026937/-minimally-invasive-and-robotic-assisted-surgical-management-of-upper-gastrointestinal-cancer
#3
REVIEW
P P Grimminger, H F Fuchs
Total minimally invasive upper gastrointestinal resections are currently mainly performed in centers. The advantages include reduction of operative trauma, magnified enlargement of the operation field and the resulting improvement in operative precision. Robotic-assisted minimally invasive esophagectomy (RAMIE) and laparoscopic/thoracoscopic minimally invasive esophagectomy (MIE) are currently the most commonly performed strategies for esophageal cancer. Laparoscopic (MIG) and robotic-assisted gastrectomy (RAG) are the equivalent procedures for gastric cancer...
October 12, 2017: Der Chirurg; Zeitschrift Für Alle Gebiete der Operativen Medizen
https://www.readbyqxmd.com/read/29017282/plasma-cytokine-predictors-of-tuberculosis-recurrence-in-antiretroviral-treated-human-immunodeficiency-virus-infected-individuals-from-durban-south-africa
#4
Aida Sivro, Lyle R McKinnon, Nonhlanhla Yende-Zuma, Santhana Gengiah, Natasha Samsunder, Salim S Abdool Karim, Kogieleum Naidoo
Background: Immune correlates of tuberculosis (TB) risk in populations infected with human immunodeficiency virus (HIV) remain understudied, despite HIV being associated with a high burden of TB disease. Here we describe plasma cytokine correlates of TB recurrence in a well-characterized cohort of HIV-infected individuals on antiretroviral therapy (ART) with a history of prior TB cure. Methods: Study participants were drawn from a prospective cohort study initiated at the conclusion of a randomized clinical trial in which individuals presented with untreated HIV infection and active pulmonary TB...
September 1, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28994628/effects-of-total-flavonoids-of-sea-buckthorn-hippophae-rhamnoides-l-on-cytotoxicity-of-nk92-mi-cells
#5
Diandong Hou, Decheng Wang, Xiande Ma, Wenna Chen, Shengnan Guo, Hongquan Guan
Sea buckthorn ( Hippophae rhamnoides L.) has multifarious medicinal properties including immunoregulatory effect. The total flavonoids of Hippophae rhamnoides L. (TFH) are the main active components isolated from berries of sea buckthorn. The aim of this study was to evaluate the effects of TFH on the cytotoxicity of NK92-MI cells and its possible mechanisms. NK92-MI cells were treated with TFH (2.5 or 5.0 mg/L) or phosphate-buffered saline (PBS) for 24 h, the cytotoxicity against K562 was detected by measuring the release of lactate dehydrogenase (LDH), expression levels of NCRs (NKp30, NKp44, NKp46) and NKG2D were detected by flow cytometry, and expression levels of perforin and granzyme B were detected by western blot...
October 1, 2017: International Journal of Immunopathology and Pharmacology
https://www.readbyqxmd.com/read/28986003/postural-tachycardia-in-hypermobile-ehlers-danlos-syndrome-a-distinct-subtype
#6
Mitchell G Miglis, Brittany Schultz, Srikanth Muppidi
INTRODUCTION: It is not clear if patients with postural tachycardia syndrome (POTS) and Ehlers-Danlos syndrome (hEDS) differ from patients with POTS due to other etiologies. We compared the results of autonomic testing and healthcare utilization in POTS patients with and without hEDS. METHODS: Patients with POTS+hEDS (n=20) and POTS controls without hypermobility (n=20) were included in the study. All patients underwent autonomic testing, and the electronic medical records were reviewed to determine the number and types of medications patients were taking, as well as the number of outpatient, emergency department, and inpatient visits over the prior year...
October 2, 2017: Autonomic Neuroscience: Basic & Clinical
https://www.readbyqxmd.com/read/28983689/is-the-answer-just-beneath-the-surface-and-other-updates-on-recent-autonomic-research
#7
EDITORIAL
Mitchell G Miglis, Srikanth Muppidi
No abstract text is available yet for this article.
October 5, 2017: Clinical Autonomic Research: Official Journal of the Clinical Autonomic Research Society
https://www.readbyqxmd.com/read/28964926/managing-postoperative-pain-after-minimally-invasive-gynecologic-surgery-in-the-era-of-the-opioid-epidemic
#8
REVIEW
Marron Wong, Stephanie Morris, Karen Wang, Khara Simpson
In this review, we examine the evidence behind non-opioid medication alternatives, peripheral nerve blocks, surgical techniques, and postoperative recovery protocols that can help minimize and effectively treat postoperative pain after minimally invasive gynecologic surgery (MIGS). Due to the depth and heterogeneity of the data, a narrative review was performed of reported interventions. A comprehensive review was performed of Pubmed, EMBASE, and the Cochrane Database with a focus on randomized controlled trials (RCT)...
September 27, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28951655/impact-of-a-fellowship-trained-minimally-invasive-gynecologic-surgeon-on-patient-outcomes
#9
Nisse V Clark, Harneet S Gujral, Kelly N Wright
BACKGROUND AND OBJECTIVES: As the performance of minimally invasive hysterectomy has increased in the United States, the need to apply outcomes measures has also increased. This study was conducted to determine the impact of a fellowship-trained minimally invasive gynecologic surgery (MIGS) specialist on patient outcomes after laparoscopic hysterectomy (LH) in a gynecology department. METHODS: This is a retrospective review of 218 patients who underwent a laparoscopic hysterectomy for benign indications at a suburban academic-affiliated tertiary care hospital with a broad patient base from 2010 to 2014...
July 2017: JSLS: Journal of the Society of Laparoendoscopic Surgeons
https://www.readbyqxmd.com/read/28936426/comparison-of-diabetic-and-non-diabetic-human-leukocytic-responses-to-different-capsule-types-of-klebsiella-pneumoniae-responsible-for-causing-pyogenic-liver-abscess
#10
I Russel Lee, Ethel Sng, Kok-Onn Lee, James S Molton, Monica Chan, Shirin Kalimuddin, Ezlyn Izharuddin, David C Lye, Sophia Archuleta, Yunn-Hwen Gan
The major risk factor for Klebsiella liver abscess (KLA) is type 2 diabetes mellitus (DM), but the immunological mechanisms involved in the increased susceptibility are poorly defined. We investigated the responses of neutrophils and peripheral blood mononuclear cells (PBMCs) to hypervirulent Klebsiella pneumoniae (hvKP), the causative agent of KLA. DNA and myeloperoxidase levels were elevated in the plasma of KLA patients compared to uninfected individuals indicating neutrophil activation, but diabetic status had no effect on these neutrophil extracellular trap (NET) biomarkers in both subject groups...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28925396/mig-6-negatively-regulates-stat3-phosphorylation-in-uterine-epithelial-cells
#11
J-Y Yoo, W S Yang, J H Lee, B G Kim, R R Broaddus, J M Lim, T H Kim, J-W Jeong
Endometrial cancer is the most common malignancy of the female genital tract. Progesterone (P4) has been used for several decades in endometrial cancer treatment, especially in women who wish to retain fertility. However, it is unpredictable which patients will respond to P4 treatment and which may have a P4-resistant cancer. Therefore, identifying the mechanism of P4 resistance is essential to improve the therapies for endometrial cancer. Mitogen-inducible gene 6 (Mig-6) is a critical mediator of progesterone receptor (PGR) action in the uterus...
September 18, 2017: Oncogene
https://www.readbyqxmd.com/read/28924343/glaucoma-surgery-in-scleromalacia-using-endoscopic-cyclophotocoagulation-where-conventional-filtration-surgery-or-angle-procedures-are-contraindicated
#12
Ian As Rodrigues, Dan Lindfield, Miles R Stanford, Saurabh Goyal
AIM: To describe the surgical management of glaucoma in a patient with severe scleromalacia, and secondary angle closure. INTRODUCTION: The management of glaucoma with coexisting scleromalacia plus secondary angle closure is challenging as most commonly performed incisional glaucoma surgery as well as minimally invasive glaucoma surgery (MIGS), which targets the drainage angle are all contraindicated. CASE REPORT: Medically refractory glaucoma in a 60-year-old male with a 30-year history of granulomatosis with polyangiitis resulting in extensive severe scleromalacia, cicatricial lower lid retraction with significant conjunctival exposure, and widespread synechial angle closure from chronic anterior uveitis was managed with combined phacoemulsification cataract surgery, and endoscopic cyclophotocoagulation (ECP)...
May 2017: Journal of Current Glaucoma Practice
https://www.readbyqxmd.com/read/28923960/igh-isotype-specific-b-cell-receptor-expression-influences-b-cell-fate
#13
Pei Tong, Alessandra Granato, Teng Zuo, Neha Chaudhary, Adam Zuiani, Seung Seok Han, Rakesh Donthula, Akritee Shrestha, Debattama Sen, Jennifer M Magee, Michael P Gallagher, Cees E van der Poel, Michael C Carroll, Duane R Wesemann
Ig heavy chain (IgH) isotypes (e.g., IgM, IgG, and IgE) are generated as secreted/soluble antibodies (sIg) or as membrane-bound (mIg) B cell receptors (BCRs) through alternative RNA splicing. IgH isotype dictates soluble antibody function, but how mIg isotype influences B cell behavior is not well defined. We examined IgH isotype-specific BCR function by analyzing naturally switched B cells from wild-type mice, as well as by engineering polyclonal Ighγ1/γ1 and Ighε/ε mice, which initially produce IgG1 or IgE from their respective native genomic configurations...
September 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28920701/absence-of-a-band-gap-at-the-interface-of-a-metal-and-highly-doped-monolayer-mos2
#14
Alexander Kerelsky, Ankur Nipane, Drew Edelberg, Dennis Wang, Xiaodong Zhou, Abdollah Motmaendadgar, Hui Gao, Saien Xie, Kibum Kang, Jiwoong Park, James Teherani, Abhay Pasupathy
High quality electrical contact to semiconducting transition metal dichalcogenides (TMDCs) such as MoS2 is key to unlocking their unique electronic and optoelectronic properties for fundamental research and device applications. Despite extensive experimental and theoretical efforts reliable ohmic contact to doped TMDCs remains elusive and would benefit from a better understanding of the underlying physics of the metal-TMDC interface. Here we present measurements of the atomic-scale energy band diagram of junctions between various metals and heavily doped monolayer MoS2 using ultrahigh vacuum scanning tunneling microscopy (UHV-STM)...
October 11, 2017: Nano Letters
https://www.readbyqxmd.com/read/28919702/micro-invasive-glaucoma-surgery-migs-a-review-of-surgical-procedures-using-stents
#15
Lutz E Pillunat, Carl Erb, Anselm Gm Jünemann, Friedemann Kimmich
Over the last decade several novel surgical treatment options and devices for glaucoma have been developed. All these developments aim to cause as little trauma as possible to the eye, to safely, effectively, and sustainably reduce intraocular pressure (IOP), to produce reproducible results, and to be easy to adopt. The term "micro-invasive glaucoma surgery (MIGS)" was used for summarizing all these procedures. Currently MIGS is gaining more and more interest and popularity. The possible reduction of the number of glaucoma medications, the ab interno approach without damaging the conjunctival tissue, and the probably safer procedures compared to incisional surgical methods may explain the increased interest in MIGS...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/28905306/erratum-to-an-update-on-implants-for-minimally-invasive-glaucoma-surgery-migs
#16
Ejaz Ansari
No abstract text is available yet for this article.
September 13, 2017: Ophthalmology and Therapy
https://www.readbyqxmd.com/read/28885246/surgical-innovations-in-glaucoma-the-transition-from-trabeculectomy-to-migs
#17
Jonathan Chou, Angela Turalba, Ambika Hoguet
No abstract text is available yet for this article.
2017: International Ophthalmology Clinics
https://www.readbyqxmd.com/read/28866045/corticosteroid-inhibits-chemokines-production-in-systemic-sclerosis-patients
#18
Andréa Tavares Dantas, Anderson Rodrigues de Almeida, Maria Clara Pinheiro Duarte Sampaio, Marina Ferraz Cordeiro, Laurindo Ferreira da Rocha, Priscilla Stela Santana de Oliveira, Michelly Cristiny Pereira, Moacyr Jesus Barreto de Melo Rego, Claudia Diniz Lopes Marques, Ivan da Rocha Pitta, Angela Luzia Branco Pinto Duarte, Maira Galdino da Rocha Pitta
In this study, we evaluated glucocorticoids (GC) effects on cytokine/chemokine levels in serum samples and peripheral blood mononuclear cell (PBMC) production from systemic sclerosis (SSc) patients. We evaluated cytokine and chemokine levels in serum samples from SSc patients taking or not taking systemic glucocorticoids. PBMCs response to methylprednisolone (MP) was examined from 15 SSc patients and 8 healthy control subjects following PBMC stimulation with anti-CD3/CD28. Cytokine (IFN-γ, TNF, IL-2, IL-4, IL-6, IL-10, and IL-17A) and chemokine (CXCL8/IL-8, CCL5/RANTES, CXCL9/MIG, CCL2/MCP-1, and CXCL10/IP-10) levels were quantified in serum and in PBMC culture supernatants by CBA or ELISA...
September 1, 2017: Steroids
https://www.readbyqxmd.com/read/28859089/flavin-monooxygenases-regulate-caenorhabditis-elegans-axon-guidance-and-growth-cone-protrusion-with-unc-6-netrin-signaling-and-rac-gtpases
#19
Mahekta R Gujar, Aubrie M Stricker, Erik A Lundquist
The guidance cue UNC-6/Netrin regulates both attractive and repulsive axon guidance. Our previous work showed that in C. elegans, the attractive UNC-6/Netrin receptor UNC-40/DCC stimulates growth cone protrusion, and that the repulsive receptor, an UNC-5:UNC-40 heterodimer, inhibits growth cone protrusion. We have also shown that inhibition of growth cone protrusion downstream of the UNC-5:UNC-40 repulsive receptor involves Rac GTPases, the Rac GTP exchange factor UNC-73/Trio, and the cytoskeletal regulator UNC-33/CRMP, which mediates Semaphorin-induced growth cone collapse in other systems...
August 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28837978/-do-we-perform-glaucoma-surgery-too-late
#20
Jörg Peter Egon Stürmer, Christoph Faschinger
As glaucoma specialists, we often ask ourselves why patients are referred so late for surgery. Usually the patient is referred in a far advanced stage of the disease after long-term conventional treatment with topical medications. By that time, substantial morphologic damage is present and the patient is disabled by far advanced visual field defects. In addition, decompensated intraocular pressure, despite maximal tolerated medical treatment, is seen. Delayed surgery due to late diagnosis is rather a rare event...
August 24, 2017: Klinische Monatsblätter Für Augenheilkunde
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