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https://www.readbyqxmd.com/read/28922717/magnetic-nanoparticle-loaded-alginate-beads-for-local-micro-actuation-of-in-vitro-tissue-constructs
#1
Awatef M Alshehri, Otto C Wilson, Bishnu Dahal, John Philip, Xiaolong Luo, Christopher B Raub
Magnetic nanoparticles (MNPs) self-align and transduce magnetic force, two properties which lead to promising applications in cell and tissue engineering. However, the toxicity of MNPs to cells which uptake them is a major impediment to applications in engineered tissue constructs. To address this problem, MNPs were embedded in millimeter-scale alginate beads, coated with glutaraldehyde cross-linked chitosan, and loaded in acellular and MDA-MB-231 cancer cell-seeded collagen hydrogels, providing local micro-actuation under an external magnetic field...
September 4, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28921946/biomimetic-magnetosomes-as-versatile-artificial-antigen-presenting-cells-to-potentiate-t-cell-based-anticancer-therapy
#2
Qianmei Zhang, Wei Wei, Peiling Wang, Liping Zuo, Feng Li, Jin Xu, Xiaobo Xi, Xiaoyong Gao, Guanghui Ma, Hai-Yan Xie
Adoptive T-cell transfer for cancer therapy relies on both effective ex vivo T cell expansion and in vivo targeting performance. One promising but challenging method for accomplishing this purpose is to construct multifunctional artificial antigen-presenting cells (aAPCs). We herein developed biomimetic magnetosomes as versatile aAPCs, wherein magnetic nanoclusters were coated with azide-engineered leucocyte membranes and then decorated with T-cell stimuli through copper-free click chemistry. These novel nano aAPCs not only exhibited high performance for antigen-specific cytotoxic T cell (CTL) expansion and stimulation but also visually and effectively guided reinfused CTLs to tumor tissues through magnetic resonance imaging and magnetic control...
September 18, 2017: ACS Nano
https://www.readbyqxmd.com/read/28921822/construction-of-human-induced-pluripotent-stem-cell-derived-oriented-bone-matrix-microstructure-by-using-in-vitro-engineered-anisotropic-culture-model
#3
Ryosuke Ozasa, Aira Matsugaki, Yoshihiro Isobe, Taro Saku, Hui-Suk Yun, Takayoshi Nakano
Bone tissue has anisotropic microstructure based on collagen/biological apatite orientation, which plays essential roles in the mechanical and biological functions of bone. However, obtaining an appropriate anisotropic microstructure during the bone regeneration process remains a great challenging. A powerful strategy for the control of both differentiation and structural development of newly-formed bone is required in bone tissue engineering, in order to realize functional bone tissue regeneration. In this study, we developed a novel anisotropic culture model by combining human induced pluripotent stem cells (hiPSCs) and artificially-controlled oriented collagen scaffold...
September 16, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28921540/imaging-stem-cell-distribution-growth-migration-and-differentiation-in-3-d-scaffolds-for-bone-tissue-engineering-using-mesoscopic-fluorescence-tomography
#4
Qinggong Tang, Charlotte Piard, Jonathan Lin, Kai Nan, Ting Guo, John Caccamese, John Fisher, Yu Chen
Regenerative medicine has emerged as an important discipline that aims to repair injury or replace damaged tissues or organs by introducing living cells or functioning tissues. Successful regenerative medicine strategies will likely depend upon a simultaneous optimization strategy for the design of biomaterials, cell-seeding methods, cell-biomaterial interactions and molecular signaling within the engineered tissues. It remains a challenge to image three-dimensional (3-D) structures and functions of the cell-seeded scaffold in mesoscopic scale (>2∼3 mm)...
September 16, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28921443/polyester-based-nanoparticles-for-the-encapsulation-of-monoclonal-antibodies
#5
Flávia Sousa, Pedro Fonte, Andreia Cruz, Patrick J Kennedy, Inês Mendes Pinto, Bruno Sarmento
Aliphatic polyesters have been widely explored for biomedical applications (e.g., drug delivery systems, biomedical devices, and tissue engineering). Recently, polyesters have been used in nanoparticle formulations for the controlled release of monoclonal antibodies (mAbs) for the enhanced efficacy of antibody-based therapy. Polyester-based nanoparticles for mAb delivery provide decreased antibody dosage, increased antibody stability and protection and longer therapeutic action, ultimately translating to an increased therapeutic index...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28921421/tissue-engineering-of-3d-organotypic-microtissues-by-acoustic-assembly
#6
Yuqing Zhu, Vahid Serpooshan, Sean Wu, Utkan Demirci, Pu Chen, Sinan Güven
There is a rapidly growing interest in generation of 3D organotypic microtissues with human physiologically relevant structure, function, and cell population in a wide range of applications including drug screening, in vitro physiological/pathological models, and regenerative medicine. Here, we provide a detailed procedure to generate structurally defined 3D organotypic microtissues from cells or cell spheroids using acoustic waves as a biocompatible and scaffold-free tissue engineering tool.
September 19, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28919526/chemical-crosslinking-of-biopolymeric-scaffolds-current-knowledge-and-future-directions-of-crosslinked-engineered-bone-scaffolds
#7
REVIEW
Ahmad Oryan, Amir Kamali, Ali Moshiri, Hossien Baharvand, Hamed Daemi
Bone tissue scaffolds made from either natural or synthetic polymers are employed to promote bone healing. However, lack of sufficient or poor mechanical properties such as low integrity and stability reduces their medical applications. Crosslinking, defined as induction of chemical or physical links among polymer chains, is a simple method generally used to modify mechanical, biological and degradation properties of hydrogels. Although crosslinking through chemical reactions improves the mechanical properties of bone substitutes, most of the reagents used for this aim demonstrate undesirable effects and may exert toxic reactions...
September 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28919509/molecular-and-macro-scale-analysis-of-enzyme-crosslinked-silk-hydrogels-for-rational-biomaterial-design
#8
Meghan McGill, Jeannine M Coburn, Benjamin P Partlow, Xuan Mu, David L Kaplan
Silk fibroin-based hydrogels have exciting applications in tissue engineering and therapeutic molecule delivery; however, their utility is dependent on their diffusive properties. The present study describes a molecular and macro-scale investigation of enzymatically-crosslinked silk fibroin hydrogels, and demonstrates that these systems have tunable crosslink density and diffusivity. We developed a liquid chromatography tandem mass spectroscopy (LC-MS/MS) method to assess the quantity and order of covalent tyrosine crosslinks in the hydrogels...
September 14, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28919507/tissue-engineered-magnetic-cell-sheet-patches-for-advanced-strategies-in-tendon-regeneration
#9
Ana I Gonçalves, Márcia T Rodrigues, Manuela E Gomes
Tendons are powerful 3D biomechanically structures combining a few cells in an intrincated and highly hierarchical niche environment. When tendon homeostasis is compromised, restoration of functionality upon injury is limited and requires alternatives to current augmentation or replacement strategies. Cell sheet technologies are a powerful tool for the fabrication of living extracellular-rich patches towards regeneration of tenotopic defects. Thus, we originally propose the development of magnetically responsive tenogenic patches through magnetic cell sheet (magCSs) technology that enable the remote control upon implantation of the tendon-mimicking constructs...
September 14, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28919377/combination-cancer-therapy-using-chimeric-antigen-receptor-engineered-natural-killer-cells-as-drug-carriers
#10
Elizabeth L Siegler, Yu Jeong Kim, Xianhui Chen, Natnaree Siriwon, John Mac, Jennifer A Rohrs, Paul D Bryson, Pin Wang
The therapeutic limitations of conventional chemotherapeutic drugs include chemo-resistance, tumor recurrence, and metastasis. Numerous nanoparticle-based active targeting approaches have emerged to enhance the intracellular concentration of drugs in tumor cells; however, efficient delivery of these systems to the tumor site while sparing healthy tissue remains elusive. Recently, much attention has been given to human immune-cell-directed nanoparticle drug delivery, because immune cells can traffic to the tumor and inflammatory sites...
August 19, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28919259/ontogenic-identification-and-analysis-of-mesenchymal-stromal-cell-populations-during-mouse-limb-and-long-bone-development
#11
Gretel Nusspaumer, Sumit Jaiswal, Andrea Barbero, Robert Reinhardt, Dana Ishay Ronen, Alexander Haumer, Thomas Lufkin, Ivan Martin, Rolf Zeller
Bone-derived mesenchymal stromal cells (MSCs) differentiate into multiple lineages including chondro- and osteogenic fates and function in establishing the hematopoietic compartment of the bone marrow. Here, we analyze the emergence of different MSC types during mouse limb and long bone development. In particular, PDGFRα(pos)SCA-1(pos) (PαS) cells and mouse skeletal stem cells (mSSCs) are detected within the PDGFRα(pos)CD51(pos) (PαCD51) mesenchymal progenitors, which are the most abundant progenitors in early limb buds and developing long bones until birth...
September 1, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28918561/marine-polysaccharides-therapeutic-efficacy-and-biomedical-applications
#12
REVIEW
Young-Eun Lee, Hyeongmin Kim, Changwon Seo, Taejun Park, Kyung Bin Lee, Seung-Yup Yoo, Seong-Chul Hong, Jeong Tae Kim, Jaehwi Lee
The ocean contains numerous marine organisms, including algae, animals, and plants, from which diverse marine polysaccharides with useful physicochemical and biological properties can be extracted. In particular, fucoidan, carrageenan, alginate, and chitosan have been extensively investigated in pharmaceutical and biomedical fields owing to their desirable characteristics, such as biocompatibility, biodegradability, and bioactivity. Various therapeutic efficacies of marine polysaccharides have been elucidated, including the inhibition of cancer, inflammation, and viral infection...
September 16, 2017: Archives of Pharmacal Research
https://www.readbyqxmd.com/read/28917940/natural-and-synthetic-polymers-bioceramics-bioactive-compounds-mediated-cell-signaling-in-bone-tissue-engineering
#13
REVIEW
S Harsha Rao, B Harini, R Pranav Kumar Shadamarshan, K Balagangadharan, N Selvamurugan
Bone is a highly integrative and dynamic tissue of the human body. It is continually remodeled by bone cells such as osteoblasts, osteoclasts. When a fraction of a bone is damaged or deformed, stem cells and bone cells under the influence of several signaling pathways regulate bone regeneration at the particular locale. Effective therapies for bone defects can be met via bone tissue engineering which employs drug delivery systems with biomaterials to enhance cellular functions by acting on signaling pathways such as Wnt, BMP, TGF-β, and Notch...
September 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28917909/development-of-poly-1-8-octanediol-citrate-chitosan-blend-films-for-tissue-engineering-applications
#14
Ehsan Zeimaran, Sara Pourshahrestani, Belinda Pingguan-Murphy, Daniel Kong, Sangeetha Vasudevaraj Naveen, Tunku Kamarul, Nahrizul Adib Kadri
Blends of poly (1, 8-octanediol citrate) (POC) and chitosan (CS) were prepared through solution casting technique. Films with different component fractions (POC/CS: 100/0, 90/10, 80/20, 70/30, 60/40, and 0/100) were successfully prepared and characterized for their mechanical, thermal, structural and morphological properties as well as biocompatibility. The incorporation of CS to POC significantly increased tensile strength and elastic modulus and presented limited influences on pH variation which is important to the biocompatibility of biomaterial implants...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917901/polysaccharides-based-injectable-hydrogel-compositing-bio-glass-for-cranial-bone-repair
#15
Xiao Bai, Shaoyu Lü, Haidi Liu, Zhen Cao, Piao Ning, Zengqiang Wang, Chunmei Gao, Boli Ni, Dongyang Ma, Mingzhu Liu
Bone disease is a public health problem around the word, and it is urgent to develop novel tissue engineering scaffolds for the complicated cranial bone regeneration. The present work developed a novel triple crosslinked polysaccharides based injectable hydrogel to composite bio-glass (BG) for cranial bone repair. Dynamic mechanical analysis showed the storage modulus (G') of the hydrogel reached to ∼4000Pa. While after compositing BG, G' exceeded 4500Pa. The degradation behavior of the hydrogel is influenced by hydrogel composition, crosslinking methods and degradation environment...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917880/intercalated-chitosan-hydroxyapatite-nanocomposites-promising-materials-for-bone-tissue-engineering-applications
#16
Muhammad Anwaar Nazeer, Emel Yilgör, Iskender Yilgör
Preparation and characterization of chitosan/hydroxyapatite (CS/HA) nanocomposites displaying an intercalated structure is reported. Hydroxyapatite was synthesized through sol-gel process. Formic acid was introduced as a new solvent to obtain stable dispersions of nano-sized HA particles in polymer solution. CS/HA dispersions with HA contents of 5, 10 and 20% by weight were prepared. Self-assembling of HA nanoparticles during the drying of the solvent cast films led to the formation of homogeneous CS/HA nanocomposites...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917869/high-aspect-ratio-phospho-calcified-rock-candy-like-cellulose-nanowhiskers-of-wastepaper-applicable-in-osteogenic-differentiation-of-hmscs
#17
Mohsen Shahrousvand, Fatemeh Ahmadi Tabar, Ehsan Shahrousvand, Amir Babaei, Mohammad Mahdi Hasani-Sadrabadi, Gity Mir Mohamad Sadeghi, Hafez Jafari, Ali Salimi
The aim of this study was to prepare cellulose nanowhiskers (CNWs) from wastepaper powder (WPP), as an environmentally friendly approach for obtaining the source material, which is a highly available and low-cost precursor for cellulose nanomaterial processing. Acid hydrolysis and calcification treatments were employed for extraction of CNWs and preparation of novel phospho-calcified cellulose nanowhiskers (PCCNWs). CNWs and PCCNWs were analyzed through optical microscopy (OM), scanning electron microscopy (SEM), Fourier-transformed infrared spectra (FTIR) and X-ray diffraction analysis (XRD)...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917626/ultrasound-assisted-liposuction-provides-a-source-for-functional-adipose-derived-stromal-cells
#18
Dominik Duscher, Zeshaan N Maan, Anna Luan, Matthias M Aitzetmüller, Elizabeth A Brett, David Atashroo, Alexander J Whittam, Michael S Hu, Graham G Walmsley, Khosrow S Houschyar, Arndt F Schilling, Hans-Guenther Machens, Geoffrey C Gurtner, Michael T Longaker, Derrick C Wan
BACKGROUND AIMS: Regenerative medicine employs human mesenchymal stromal cells (MSCs) for their multi-lineage plasticity and their pro-regenerative cytokine secretome. Adipose-derived mesenchymal stromal cells (ASCs) are concentrated in fat tissue, and the ease of harvest via liposuction makes them a particularly interesting cell source. However, there are various liposuction methods, and few have been assessed regarding their impact on ASC functionality. Here we study the impact of the two most popular ultrasound-assisted liposuction (UAL) devices currently in clinical use, VASER (Solta Medical) and Lysonix 3000 (Mentor) on ASCs...
September 13, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28917530/overcoming-key-biological-barriers-to-cancer-drug-delivery-and-efficacy
#19
Susy M Kim, Peggy H Faix, Jan E Schnitzer
Poor delivery efficiency continues to hamper the effectiveness of cancer therapeutics engineered to destroy solid tumors using different strategies such as nanocarriers, targeting agents, and matching treatments to specific genetic mutations. All contemporary anti-cancer agents are dependent upon passive transvascular mechanisms for their delivery into solid tumors. The therapeutic efficacies of our current drug arsenal could be significantly improved with an active delivery strategy. Here, we discuss how drug delivery and therapeutic efficacy is greatly hindered by barriers presented by the vascular endothelial cell layer and by the aberrant nature of tumor blood vessels in general...
September 13, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28917149/a-comparison-study-between-electrospun-polycaprolactone-and-piezoelectric-poly-3-hydroxybutyrate-co-3-hydroxyvalerate-scaffolds-for-bone-tissue-engineering
#20
Svetlana N Gorodzha, Albert R Muslimov, Dina S Syromotina, Alexander S Timin, Nikolai Y Tcvetkov, Kirill V Lepik, Aleksandra V Petrova, Maria A Surmeneva, Dmitry A Gorin, Gleb B Sukhorukov, Roman A Surmenev
In this study, bone scaffolds composed of polycaprolactone (PCL), piezoelectric poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and a combination of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and silicate containing hydroxyapatite (PHBV-SiHA) were successfully fabricated by a conventional electrospinning process. The morphological, chemical, wetting and biological properties of the scaffolds were examined. All fabricated scaffolds are composed of randomly oriented fibres with diameters from 800nm to 12μm...
September 5, 2017: Colloids and Surfaces. B, Biointerfaces
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