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https://www.readbyqxmd.com/read/27933305/robust-generation-of-transgenic-mice-by-simple-hypotonic-solution-mediated-delivery-of-transgene-in-testicular-germ-cells
#1
Abul Usmani, Nirmalya Ganguli, Subodh K Jain, Nilanjana Ganguli, Rajesh Kumar Sarkar, Mayank Choubey, Mansi Shukla, Hironmoy Sarkar, Subeer S Majumdar
Our ability to decipher gene sequences has increased enormously with the advent of modern sequencing tools, but the ability to divulge functions of new genes have not increased correspondingly. This has caused a remarkable delay in functional interpretation of several newly found genes in tissue and age specific manner, limiting the pace of biological research. This is mainly due to lack of advancements in methodological tools for transgenesis. Predominantly practiced method of transgenesis by pronuclear DNA-microinjection is time consuming, tedious, and requires highly skilled persons for embryo-manipulation...
2016: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/27933251/robust-image-hashing-using-ring-partition-pgnmf-and-local-features
#2
Ram Kumar Karsh, R H Laskar, Bhanu Bhai Richhariya
BACKGROUND: Image authentication is one of the challenging research areas in the multimedia technology due to the availability of image editing tools. Image hash may be used for image authentication which should be invariant to perceptually similar image and sensitive to content changes. The challenging issue in image hashing is to design a system which simultaneously provides rotation robustness, desirable discrimination, sensitivity and localization of forged area with minimum hash length...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27932164/retzius-space-preservation-technique-for-robotic-assisted-laparoscopic-radical-prostatectomy-in-a-kidney-transplant-patient-first-case-in-thailand-and-our-first-experience
#3
P Jenjitranant, P Sangkum, P Sirisreetreerux, W Viseshsindh, S Patcharatrakul, W Kongcharoensombat
OBJECTIVE: The aim of this work was to report our experience in robotic-assisted laparoscopic radical prostatectomy for the treatment of localized prostate cancer in a kidney transplant recipient. METHODS: A 73-year-old man with chronic renal failure underwent living-donor kidney transplantation (KT) in 1993. His baseline creatinine after KT was ∼1.2 mg/dL. He developed lower urinary tract symptoms in 1999. He was diagnosed with benign prostatic hyperplasia and treated accordingly...
November 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27931947/transection-of-the-omohyoid-muscle-as-an-aid-during-vagal-nerve-stimulator-implantation
#4
June Yowtak, Patrick Jenkins, Cole Giller
BACKGROUND: Exposure of the carotid sheath during VNS implantation is usually straightforward, but can be difficult for patients with a large body habitus. In addition, the exposure must be done with care if the surgeon wishes to keep the vagus nerve in situ without using retractors that might impair access. OBJECTIVE: To describe the use of the omohyoid muscle as a landmark for the jugular vein, and to report how transection of the omohyoid can facilitate a rapid and wide exposure of the carotid sheath...
December 5, 2016: World Neurosurgery
https://www.readbyqxmd.com/read/27929455/neuropharmacological-manipulation-of-restrained-and-free-flying-honey-bees-apis-mellifera
#5
Eirik Søvik, Jenny A Plath, Jean-Marc Devaud, Andrew B Barron
Honey bees demonstrate astonishing learning abilities and advanced social behavior and communication. In addition, their brain is small, easy to visualize and to study. Therefore, bees have long been a favored model amongst neurobiologists and neuroethologists for studying the neural basis of social and natural behavior. It is important, however, that the experimental techniques used to study bees do not interfere with the behaviors being studied. Because of this, it has been necessary to develop a range of techniques for pharmacological manipulation of honey bees...
November 26, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27929070/all-dielectric-ultrathin-conformal-metasurfaces-lensing-and-cloaking-applications-at-532%C3%A2-nm-wavelength
#6
Jierong Cheng, Samad Jafar-Zanjani, Hossein Mosallaei
Metasurfaces are ideal candidates for conformal wave manipulation on curved objects due to their low profiles and rich functionalities. Here we design and analyze conformal metasurfaces for practical optical applications at 532 nm visible band for the first time. The inclusions are silicon disk nanoantennas embedded in a flexible supporting layer of polydimethylsiloxane (PDMS). They behave as local phase controllers in subwavelength dimensions for successful modification of electromagnetic responses point by point, with merits of high efficiency, at visible regime, ultrathin films, good tolerance to the incidence angle and the grid stretching due to the curvy substrate...
December 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27928670/tensile-strength-and-failure-load-of-sutures-for-robotic-surgery
#7
Ahmad Abiri, Omeed Paydar, Anna Tao, Megan LaRocca, Kang Liu, Bradley Genovese, Robert Candler, Warren S Grundfest, Erik P Dutson
BACKGROUND: Robotic surgical platforms have seen increased use among minimally invasive gastrointestinal surgeons (von Fraunhofer et al. in J Biomed Mater Res 19(5):595-600, 1985. doi: 10.1002/jbm.820190511 ). However, these systems still suffer from lack of haptic feedback, which results in exertion of excessive force, often leading to suture failures (Barbash et al. in Ann Surg 259(1):1-6, 2014. doi: 10.1097/SLA.0b013e3182a5c8b8 ). This work catalogs tensile strength and failure load among commonly used sutures in an effort to prevent robotic surgical consoles from exceeding identified thresholds...
December 7, 2016: Surgical Endoscopy
https://www.readbyqxmd.com/read/27926850/background-suppression-in-imaging-gold-nanorods-through-detection-of-anti-stokes-emission
#8
Aquiles Carattino, Veer I P Keizer, Marcel J M Schaaf, Michel Orrit
Metallic nanoparticles have opened the possibility of imaging, tracking, and manipulating biological samples without time limitations. Their low photoluminescence quantum yield, however, makes them hard to detect under high background conditions. In this study we show that it is possible to image gold nanorods by detecting their anti-Stokes emission under resonant excitation. We show that even in the membrane of a cell containing the fluorescent dye Atto 647N, the signal/background of the anti-Stokes emission can be >10, while it is impossible to image the particles with the Stokes emission...
December 6, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27926672/mechanisms-predisposing-factors-and-prognosis-of-intra-operative-vertebral-subluxation-during-pedicle-subtraction-osteotomy-in-surgical-correction-of-thoracolumbar-kyphosis-secondary-to-ankylosing-spondylitis
#9
Bang-Ping Qian, Sai-Hu Mao, Jun Jiang, Bin Wang, Yong Qiu
STUDY DESIGN: A retrospective study. OBJECTIVE: To analyze the mechanisms, predisposing factors and prognosis of the intraoperative vertebral subluxation (VS) during pedicle subtraction osteotomy (PSO) for thoracolumbar kyphosis secondary to ankylosing spondylitis (AS). SUMMARY OF BACKGROUND DATA: VS is one of the most daunting challenges that surgeons encounter during PSO closure, especially in AS patients with ankylosed and mostly osteoporotic spine...
December 6, 2016: Spine
https://www.readbyqxmd.com/read/27926437/involvement-of-opioid-signaling-in-food-preference-and-motivation-studies-in-laboratory-animals
#10
I Morales, L Font, P J Currie, R Pastor
Motivation is a complex neurobiological process that initiates, directs, and maintains goal-oriented behavior. Although distinct components of motivated behavior are difficult to investigate, appetitive and consummatory phases of motivation are experimentally separable. Different neurotransmitter systems, particularly the mesolimbic dopaminergic system, have been associated with food motivation. Over the last two decades, however, research focusing on the role of opioid signaling has been particularly growing in this area...
2016: Progress in Brain Research
https://www.readbyqxmd.com/read/27926352/3d-laparoscopy-in-neonates-and-infants
#11
Yury Kozlov, Konstantin Kovalkov, Vladimir Nowogilov
BACKGROUND: This study focuses on the successful application of three-dimensional (3D) laparoscopic surgeries in the treatment of congenital anomalies and acquired diseases in the young pediatric population. The purpose of this scientific work consists in highlighting the spectrum, indications, applicability, and effectiveness of 3D endosurgery in children. METHODS: Our experience is based on 110 endosurgical procedures performed in neonates and infants in the 3D format between January 2014 and May 2015...
December 2016: Journal of Laparoendoscopic & Advanced Surgical Techniques. Part A
https://www.readbyqxmd.com/read/27925713/ramsey-interference-with-single-photons
#12
Stéphane Clemmen, Alessandro Farsi, Sven Ramelow, Alexander L Gaeta
Interferometry using discrete energy levels of nuclear, atomic, or molecular systems is the foundation for a wide range of physical phenomena and enables powerful techniques such as nuclear magnetic resonance, electron spin resonance, Ramsey-based spectroscopy, and laser or maser technology. It also plays a unique role in quantum information processing as qubits may be implemented as energy superposition states of simple quantum systems. Here, we demonstrate quantum interference involving energy states of single quanta of light...
November 25, 2016: Physical Review Letters
https://www.readbyqxmd.com/read/27924097/editorial-guidewire-trauma-a-key-component-of-post-ercp-pancreatitis-that-is-best-controlled-by-the-endoscopist
#13
Indu Srinivasan, Martin L Freeman
Recent studies have revealed that deep pancreatic duct wire passage, even in the absence of contrast injection, is a major technique-related culprit in post-ERCP pancreatitis, unless followed by placement of a pancreatic stent. As guidewire cannulation becomes increasingly widespread, precise control of the guidewire is thus critical. The first randomized trial to compare endoscopist-controlled wire guided vs. assistant-controlled bile duct cannulation has shown a significant reduction in post ERCP pancreatitis when the physician manipulates the wire...
December 2016: American Journal of Gastroenterology
https://www.readbyqxmd.com/read/27923729/ph-gradient-ion-exchange-microbial-cell-chromatography-as-a-simple-method-for-microbial-separation
#14
Yoshiteru Aoi, Yuji Kaneko, Satoshi Tsuneda
Selective separation of specific microbial types from a heterogeneous microbial population, such as an environmental microbial community, is an important process for microbial research and biotechnological industries. In the present study, pH-gradient ion-exchange microbial cell chromatography (PIE-MCC) was developed as a new method for microbial separation. The proposed method enables target microorganisms to be separated from a microbial community based on differences in microbial surface characteristics, because these characteristics, such as the ζ (zeta)-potential, vary among microbial cells...
December 3, 2016: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/27920582/surgeons-early-experience-with-the-acessa%C3%A2-procedure-gaining-proficiency-with-new-technology
#15
Kelli M Braun, Mark Sheridan, Erin Z Latif, Lexy Regush, Anet Maksymowicz, Laura Weins, Mohamed A Bedaiwy, Nerissa Tyson, Marilyn J Davidson, Barry H Sanders
PURPOSE: Successful adoption of a new surgical procedure varies among practicing surgeons, and skill acquisition depends on the surgeon's innate ability, the complexity of the technique, and training. We report intraoperative and near-term postoperative outcomes from the Acessa procedure conducted by minimally invasive gynecologic surgeons new to Acessa, and report the surgeons' experiences during the training period. PATIENTS AND METHODS: The study was designed as a postmarket, prospective, single-arm, multicenter analysis of operative and early postoperative outcomes after proctored surgical training with the Acessa device and procedure (laparoscopic ultrasound-guided radiofrequency volumetric thermal ablation of symptomatic fibroids) in premenopausal, menstruating women as conducted in community and university hospitals in the USA and Canada...
2016: International Journal of Women's Health
https://www.readbyqxmd.com/read/27919637/critical-hand-ischemia-treatment-via-orbital-atherectomy-a-single-center-observational-retrospective-analysis
#16
Abdul Bahro, Zsuzsanna Igyarto, Brad Martinsen
BACKGROUND/PURPOSE: Critical hand ischemia (CHI) can be devastating and may result in amputation. Distal vessel calcification has been shown to be a major factor in causing CHI. Atherectomy in the upper extremities is not typically considered due to the small anatomy; however, the Diamondback 360° Peripheral Orbital Atherectomy System (OAS) (Cardiovascular Systems, Inc.) can access treatment areas with a reference vessel diameter of 1.5mm. METHODS/MATERIALS: A retrospective, observational, single center (Merit Health Center, Jackson, MS) analysis of 11 CHI patients with calcific disease of the radial artery treated with orbital atherectomy (OAS) was completed...
October 29, 2016: Cardiovascular Revascularization Medicine: Including Molecular Interventions
https://www.readbyqxmd.com/read/27917188/crispr-cas9-tool-for-qualitative-and-quantitative-plant-genome-editing
#17
REVIEW
Ali Noman, Muhammad Aqeel, Shuilin He
Recent developments in genome editing techniques have aroused substantial excitement among agricultural scientists. These techniques offer new opportunities for developing improved plant lines with addition of important traits or removal of undesirable traits. Increased adoption of genome editing has been geared by swiftly developing Clustered regularly interspaced short palindromic repeats (CRISPR). This is appearing as driving force for innovative utilization in diverse branches of plant biology. CRISPR-Cas9 mediated genome editing is being used for rapid, easy and efficient alteration of genes among diverse plant species...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27916352/robotic-liver-resection-including-the-posterosuperior-segments-initial-experience
#18
Carolijn L M A Nota, I Quintus Molenaar, Richard van Hillegersberg, Inne H M Borel Rinkes, Jeroen Hagendoorn
BACKGROUND: Robot-assisted laparoscopy has been introduced to overcome the limitations of conventional laparoscopy. This technique has potential advantages over laparoscopy, such as increased dexterity, three-dimensional view, and a magnified view of the operative field. Therefore, improved dexterity may make a robotic system particularly suited for liver resections, which require nonlinear manipulation, such as curved parenchymal transection, hilar dissection, and resection of posterosuperior segments...
November 2016: Journal of Surgical Research
https://www.readbyqxmd.com/read/27916241/no-touch-saphenous-vein-harvesting-may-improve-further-the-patency-of-saphenous-vein-composite-grafts-early-outcomes-and-1-year-angiographic-results
#19
Yong Han Kim, Hong Chul Oh, Jae Woong Choi, Ho Young Hwang, Ki-Bong Kim
BACKGROUND: Harvesting technique and surgical strategy may affect patency rates of saphenous vein (SV) grafts in coronary artery bypass graft surgery (CABG). We evaluated early clinical outcomes and 1-year graft patency rates of SV grafts that were harvested with a no-touch technique and used as composite grafts. METHODS: Three hundred sixty-eight patients who underwent off-pump CABG using SV composite grafts based on the left internal thoracic artery were studied...
December 1, 2016: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/27915157/double-injection-single-detection-asymmetric-flow-injection-manifold-for-spectrophotometric-determination-of-ascorbic-acid-and-uric-acid-selection-the-optimal-conditions-by-mcdm-approach-based-on-different-criteria-weighting-methods
#20
Samira Boroumand, Mansour Arab Chamjangali, Ghadamali Bagherian
A simple and sensitive double injection/single detector flow injection analysis (FIA) method is proposed for the simultaneous kinetic determination of ascorbic acid (AA) and uric acid (UA). This method is based upon the difference between the rates of the AA and UA reactions with Fe(3+) in the presence of 1, 10-phenanthroline (phen). The absorbance of Fe(2+)/1, 10-phenanthroline (Fe-phen) complex obtained as the product was measured spectrophotometrically at 510nm. To reach a good accuracy in the differential kinetic determination via the mathematical manipulations of the transient signals, different criteria were considered in the selection of the optimum conditions...
November 20, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
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