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https://www.readbyqxmd.com/read/28429819/effects-of-dielectrophoresis-on-thrombogenesis-in-human-whole-blood
#1
Steven Kinio, James Mills
Thrombogenesis (blood clot formation) is a major barrier to the development of biomedical devices that interface with blood. Although state-of-the-art chemically and pharmacologically mediated clot mitigation strategies are effective, some limitations of such approaches include depletion of active agents, or adverse reactions in patients. Increased clotting protein adsorption and platelet adhesion, which occurs when artificial surfaces are exposed to blood, results in enhanced clot formation on artificial surfaces...
April 21, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28429391/technical-note-direct-measurement-of-continuous-tmr-data-with-a-1d-tank-and-automated-couch-movements
#2
Nels C Knutson, Matthew C Schmidt, Matthew D Belley, Ngoc Nguyen, H Harold Li, Erno Sajo, Michael Price
PURPOSE: Real-time dynamic control of the linear accelerator, couch, and imaging parameters during radiation delivery was investigated as a novel technique for acquiring tissue maximum ratio (TMR) data. METHODS: TrueBeam Developer Mode (Varian Medical Systems, Palo Alto, CA) was used to control the linear accelerator using the Extensible Markup Language (XML). A single XML file was used to dynamically manipulate the machine, couch, and imaging parameters during radiation delivery...
April 21, 2017: Medical Physics
https://www.readbyqxmd.com/read/28426751/the-role-of-microsporidian-polar-tube-protein-4-ptp4-in-host-cell-infection
#3
Bing Han, Valérie Polonais, Tatsuki Sugi, Rama Yakubu, Peter M Takvorian, Ann Cali, Keith Maier, Mengxian Long, Matthew Levy, Herbert B Tanowitz, Guoqing Pan, Frédéric Delbac, Zeyang Zhou, Louis M Weiss
Microsporidia have been identified as pathogens that have important effects on our health, food security and economy. A key to the success of these obligate intracellular pathogens is their unique invasion organelle, the polar tube, which delivers the nucleus containing sporoplasm into host cells during invasion. Due to the size of the polar tube, the rapidity of polar tube discharge and sporoplasm passage, and the absence of genetic techniques for the manipulation of microsporidia, study of this organelle has been difficult and there is relatively little known regarding polar tube formation and the function of the proteins making up this structure...
April 20, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28425096/influence-of-coral-cover-and-structural-complexity-on-the-accuracy-of-visual-surveys-of-coral-reef-fish-communities
#4
D J Coker, J P Nowicki, N A J Graham
Using manipulated patch reefs with combinations of varying live-coral cover (low, medium and high) and structural complexity (low and high), common community metrics (abundance, diversity, richness and community composition) collected through standard underwater visual census techniques were compared with exhaustive collections using a fish anaesthetic (clove oil). This study showed that reef condition did not influence underwater visual census estimates at a community level, but reef condition can influence the detectability of some small and cryptic species and this may be exacerbated if surveys are conducted on a larger scale...
April 19, 2017: Journal of Fish Biology
https://www.readbyqxmd.com/read/28424774/cardiac-radionuclide-imaging-in-rodents-a-review-of-methods-results-and-factors-at-play
#5
Francesco Cicone, David Viertl, Ana Maria Quintela Pousa, Thibaut Denoël, Silvano Gnesin, Francesco Scopinaro, Marie-Catherine Vozenin, John O Prior
The interest around small-animal cardiac radionuclide imaging is growing as rodent models can be manipulated to allow the simulation of human diseases. In addition to new radiopharmaceuticals testing, often researchers apply well-established probes to animal models, to follow the evolution of the target disease. This reverse translation of standard radiopharmaceuticals to rodent models is complicated by technical shortcomings and by obvious differences between human and rodent cardiac physiology. In addition, radionuclide studies involving small animals are affected by several extrinsic variables, such as the choice of anesthetic...
2017: Frontiers in Medicine
https://www.readbyqxmd.com/read/28422856/a-cross-sectional-evaluation-of-meditation-experience-on-electroencephalography-data-by-artificial-neural-network-and-support-vector-machine-classifiers
#6
Yu-Hao Lee, Ya-Ju Hsieh, Yung-Jong Shiah, Yu-Huei Lin, Chiao-Yun Chen, Yu-Chang Tyan, JiaCheng GengQiu, Chung-Yao Hsu, Sharon Chia-Ju Chen
To quantitate the meditation experience is a subjective and complex issue because it is confounded by many factors such as emotional state, method of meditation, and personal physical condition. In this study, we propose a strategy with a cross-sectional analysis to evaluate the meditation experience with 2 artificial intelligence techniques: artificial neural network and support vector machine. Within this analysis system, 3 features of the electroencephalography alpha spectrum and variant normalizing scaling are manipulated as the evaluating variables for the detection of accuracy...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28422649/visual-servoed-three-dimensional-rotation-control-in-zebrafish-larva-heart-microinjection-system
#7
Songlin Zhuang, Weiyang Lin, Jie Zhong, Gefei Zhang, Li Li, Jianbin Qiu, Huijun Gao
OBJECTIVE: Zebrafish larva heart microinjection is a widely used technique in cardiac disease study. Compared with intensively researched rotation control of spherical or nearly spherical targets with clear structures, such as cells and embryos, 3-D rotation control of zebrafish larva demands new techniques due to its non-transparent structures and irregular outlines. METHODS: In this paper, we present a vision-servo system to automate the rotation process of zebrafish larva body...
April 12, 2017: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/28421983/comparative-study-between-multivariate-and-univariate-analysis-of-two-antidiabetic-combinations
#8
Maha F Abdel-Ghany, Omar Abdel-Aziz, Miriam F Ayad, Mariam M Tadros
New multivariate and univariate methods were developed for the analysis of two novel gliptin combinations by manipulating the zero-order and ratio spectra of empagliflozin and linagliptin in combination, with application on Glyxambi(®) tablets, and of alogliptin and pioglitazone in combination, with application on Oseni(®) tablets. Linearity ranges for chemometric approaches using principal component regression and partial least-squares were found to be 2–10, 2.5–12.5, 5–15, and 5–25 μg/mL for empagliflozin, linagliptin, alogliptin, and pioglitazone, respectively, whereas the respective linearity ranges for the spectrophotometric approaches were found to be 5–15, 2–12, 5–15, and 5–15 μg/mL...
April 18, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28418623/an-efficient-method-for-gene-knock-down-by-rna-interference-in-human-skin-mast-cells
#9
Tarek Hazzan, Sven Guhl, Metin Artuc, Kristin Franke, Margitta Worm, Torsten Zuberbier, Magda Babina
Mast cells (MCs) from human skin have been notoriously resistant to gene manipulation, and a method to knock-down gene expression in in situ differentiated MCs is highly desired. The Dharmacon Accell(®) transfection system proved successful on several "difficult-to-transfect" cells. In the present work, we therefore tested this method on skin-derived MCs using different siRNA entities. The siRNA was readily taken up, followed by pronounced, specific reduction of gene and protein expression. Hence, we present the first efficient technique for the manipulation of gene expression in primary skin MCs ex vivo, which combines high transfection rates with retained cell viability...
April 18, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28417604/rotational-atherectomy-in-the-subadventitial-space-to-allow-safe-and-successful-chronic-total-occlusion-recanalization-pushing-the-limit-further
#10
Giuliana Capretti, Mauro Carlino, Antonio Colombo, Lorenzo Azzalini
Dissection and re-entry (DR) techniques have played a key role in the increase of success rates of chronic total occlusion (CTO) recanalization. DR usually allows wiring complex occlusions, even in case of important calcification. In extreme cases, such as in balloon failure-to-cross, rotational atherectomy (RA) might be decisive. However, according to experts' recommendations, RA should not be performed in dissection planes because of the high risk of perforation and further extending the dissection, so that its use after DR might be limited...
April 18, 2017: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/28416832/-minimally-invasive-plate-osteosynthesis-for-treatment-of-proximal-humeral-fractures-through-anterolateral-acromial-approach
#11
Q Zhang, N Sun, Q Huang, S W Zhu
OBJECTIVE: To investigate the feasibility, surgical technique and effect of osteosynthesis for proximal humerus fractures through the minimal incision anterolateral acromion. METHODS: In this study, 32 patients of proximal humeral fractures were selected and treated through the minimal incision antero-lateral acromion between January 2012 and October 2014. Of these patients, there were 19 males and 13 females, with mean age 46 years old (range: 22-78 years). The mechanism of injury was a trauma of simple fall for 21 patients and motor vehicle accident for 11 patients...
April 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28414697/feasibility-of-transvesical-robotic-vvf-repair-in-porcine-model
#12
Jang Hee Han, Hong-Wook Kim, Koon Ho Rha, Jang Hwan Kim
Extraperitoneal transvesicoscopic vesicovaginal fistula (VVF) repair has received limited use because of its narrow working space, longer operation time, and technical difficulty. The present study describes the feasibility of robotic-assisted transvesicoscopic VVF repair in an animal model. Two Yorkshire swine underwent robotic-assisted laparoscopic (RAL) VVF repair. With the 4 trocars, an artificial VVF was made in the supratrigonal area and VVF repair was performed in 3 layers as in open VVF repair methods...
April 14, 2017: Surgical Laparoscopy, Endoscopy & Percutaneous Techniques
https://www.readbyqxmd.com/read/28413707/disrupting-dorsolateral-prefrontal-cortex-by-rtms-reduces-the-p300-based-marker-of-deception
#13
Inga Karton, Talis Bachmann
OBJECTIVE: Quite many studies have revealed certain brain-process signatures indicative of subject's deceptive behavior. These signatures are neural correlates of deception. However, much less is known about whether these signatures can be modified by noninvasive brain stimulation techniques representing methods of causal intervention of brain processes and the corresponding behavior. Our purpose was to explore whether such methods have an effect on these signatures. METHODS: It is well known that electroencephalographic event-related potential component, P300, is sensitive to perception of critical items in a concealed information test, one of the central methods in deception studies...
April 2017: Brain and Behavior
https://www.readbyqxmd.com/read/28413613/the-rise-of-photoresponsive-protein-technologies-applications-in-vivo-a-spotlight-on-zebrafish-developmental-and-cell-biology
#14
REVIEW
Renee Wei-Yan Chow, Julien Vermot
The zebrafish ( Danio rerio) is a powerful vertebrate model to study cellular and developmental processes in vivo. The optical clarity and their amenability to genetic manipulation make zebrafish a model of choice when it comes to applying optical techniques involving genetically encoded photoresponsive protein technologies. In recent years, a number of fluorescent protein and optogenetic technologies have emerged that allow new ways to visualize, quantify, and perturb developmental dynamics. Here, we explain the principles of these new tools and describe some of their representative applications in zebrafish...
2017: F1000Research
https://www.readbyqxmd.com/read/28411645/polymer-based-protein-engineering-synthesis-and-characterization-of-armored-high-graft-density-polymer-protein-conjugates
#15
Sheiliza Carmali, Hironobu Murata, Chad Cummings, Krzysztof Matyjaszewski, Alan J Russell
Atom transfer radical polymerization (ATRP) from the surface of a protein can generate remarkably dense polymer shells that serve as armor and rationally tune protein function. Using straightforward chemistry, it is possible to covalently couple or display multiple small molecule initiators onto a protein surface. The chemistry is fine-tuned to be sequence specific (if one desires a single targeted site) at controlled density. Once the initiator is anchored on the protein surface, ATRP is used to grow polymers on protein surface, in situ...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28410359/intraoperative-s-stamp-enabled-rescue-of-3-inverted-descemet-membrane-endothelial-keratoplasty-grafts
#16
Peter B Veldman, Zachary M Mayko, Michael D Straiko, Mark A Terry
PURPOSE: To report the clinical outcomes of 3 Descemet membrane endothelial keratoplasty (DMEK) grafts, in which an upside down DMEK graft orientation was discovered after SF6 gas insufflation of the anterior chamber and the orientation was then corrected intraoperatively. METHODS: A total of 371 consecutive DMEK cases using an S-stamp were analyzed and found to include 3 cases in which the DMEK graft was initially elevated with SF6 gas in an upside down orientation, as demonstrated intraoperatively by the S-stamp...
April 12, 2017: Cornea
https://www.readbyqxmd.com/read/28410328/manipulation-of-muscle-creatine-and-glycogen-changes-dual-x-ray-absorptiometry-estimates-of-body-composition
#17
Julia L Bone, Megan L Ross, Kristyen A Tomcik, Nikki A Jeacocke, Will G Hopkins, Louise M Burke
Standardizing a dual x-ray absorptiometry (DXA) protocol is thought to provide a reliable measurement of body composition. PURPOSE: We investigated the effects of manipulating muscle glycogen and creatine content independently and additively on DXA estimates of lean mass. METHOD: Eighteen well-trained male cyclists undertook a parallel group application of creatine loading (n = 9) (20 g·d for 5 d loading; 3 g·d maintenance) or placebo (n = 9) with crossover application of glycogen loading (12 v 6 g·kg BM per day for 48 h) as part of a larger study involving a glycogen-depleting exercise protocol...
May 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/28410135/custom-built-tools-for-the-study-of-deer-antler-biology
#18
Wenhui Chu, Haiping Zhao, Junde Li, Chunyi Li
Deer antlers can be developed into multiple novel models to study growth and development of tissues for biomedical research. To facilitate this process, we have invented and further refined five custom-built tools through three decades of antler research. These are: 1. Pedicle growth detector to pinpoint the timing when pedicle growth is initiated, thus stimuli for pedicle and first antler formation can be investigated and identified. 2. Thin periosteum slice cutter to thinly slice (0.2mm or 0.7 mm thick) a whole piece of antlerogenic periosteum (AP) or pedicle periosteum (PP), which facilitates gene delivery into cells resident in these tissues, thus making transgenic antlers possible...
June 1, 2017: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/28409988/control-of-spatial-correlations-between-rydberg-excitations-using-rotary-echo
#19
N Thaicharoen, A Schwarzkopf, G Raithel
We manipulate correlations between Rydberg excitations in cold atom samples using a rotary-echo technique in which the phase of the excitation pulse is flipped at a selected time during the pulse. The correlations are due to interactions between the Rydberg atoms. We measure the resulting change in the spatial pair correlation function of the excitations via direct position-sensitive atom imaging. For zero detuning of the lasers from the interaction-free Rydberg-excitation resonance, the pair-correlation value at the most likely nearest-neighbor Rydberg-atom distance is substantially enhanced when the phase is flipped at the middle of the excitation pulse...
March 31, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28409446/isolation-of-peroxisomes-from-rat-liver-and-cultured-hepatoma-cells-by-density-gradient-centrifugation
#20
Andreas Manner, Markus Islinger
Subcellular fractionation is still a valuable technique to unravel organelle-specific proteomes, validate the location of uncharacterized proteins, or to functionally analyze import and metabolism in individual subcellular compartments. In this respect, density gradient centrifugation still represents a very classic, indispensable technique to isolate and analyze peroxisomes. Here, we present two independent protocols for the purification of peroxisomes from either liver tissue or the HepG2 hepatoma cell line...
2017: Methods in Molecular Biology
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