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https://www.readbyqxmd.com/read/28102576/printable-functional-chips-based-on-nanoparticle-assembly
#1
REVIEW
Yu Huang, Wenbo Li, Meng Qin, Haihua Zhou, Xingye Zhang, Fengyu Li, Yanlin Song
With facile manufacturability and modifiability, impressive nanoparticles (NPs) assembly applications were performed for functional patterned devices, which have attracted booming research attention due to their increasing applications in high-performance optical/electrical devices for sensing, electronics, displays, and catalysis. By virtue of easy and direct fabrication to desired patterns, high throughput, and low cost, NPs assembly printing is one of the most promising candidates for the manufacturing of functional micro-chips...
January 2017: Small
https://www.readbyqxmd.com/read/28098749/a-semipersistent-plant-virus-differentially-manipulates-feeding-behaviors-of-different-sexes-and-biotypes-of-its-whitefly-vector
#2
Shaohua Lu, Jingjing Li, Xueli Wang, Danyang Song, Rune Bai, Yan Shi, Qinsheng Gu, Yen-Wen Kuo, Bryce W Falk, Fengming Yan
It is known that plant viruses can change the performance of their vectors. However, there have been no reports on whether or how a semipersistent plant virus manipulates the feeding behaviors of its whitefly vectors. Cucurbit chlorotic yellows virus (CCYV) (genus Crinivirus, family Closteroviridae) is an emergent plant virus in many Asian countries and is transmitted specifically by B and Q biotypes of tobacco whitefly, Bemisia tabaci (Gennadius), in a semipersistent manner. In the present study, we used electrical penetration graph (EPG) technique to investigate the effect of CCYV on the feeding behaviors of B...
January 13, 2017: Viruses
https://www.readbyqxmd.com/read/28098059/laparoscopic-ileocecal-resection-in-acute-and-chronic-presentations-of-crohn-s-disease-a-single-center-experience
#3
G Cocorullo, R Tutino, N Falco, G Salamone, T Fontana, L Licari, G Gulotta
INTRODUCTION: The terminal ileum is the most involved tract in Crohn's disease. The obstruction in this location is the most frequent complication. Acute or chronic presentations can occur. Surgery finds a role in the management of chronic strictures and in acute clinical presentations with complications not improving with conservative therapy. PATIENTS AND METHODS: We investigate the outcome of patients with obstruction of the ileo-cecal bowel tract laparoscopically managed...
September 2017: Il Giornale di Chirurgia
https://www.readbyqxmd.com/read/28096585/comparison-of-three-insertion-techniques-of-proseal-laryngeal-mask-airway-a-randomized-clinical-trial
#4
Kadirehally Bheemanna Nalini, Shivanna Shivakumar, Shivashankar Archana, Doddagavanahalli Channaiah Sandhya Rani, Chadalavada Venkata Rama Mohan
BACKGROUND AND AIMS: We aimed to compare three techniques for insertion of ProSeal laryngeal mask airway (PLMA). MATERIAL AND METHODS: Two hundred ten patients (American Society of Anaethesiologists I-II, aged 18-60 years) undergoing general anesthesia using the PLMA as an airway management device were randomly allocated to digital (D), rotational (R), or pharyngoscopic (P) techniques. In the D group (n = 70), the PLMA insertion was performed by using digital manipulation...
October 2016: Journal of Anaesthesiology, Clinical Pharmacology
https://www.readbyqxmd.com/read/28092537/detail-enhanced-multi-scale-exposure-fusion
#5
Zhengguo Li, Zhe Wei, Changyun Wen, Jinghong Zheng
Multi-scale exposure fusion is an effective image enhancement technique for a high dynamic range (HDR) scene. In this paper, a new multi-scale exposure fusion algorithm is proposed to merge differently exposed low dynamic range (LDR) images by using the weighted guided image filter (WGIF) to smooth the Gaussian pyramids of weight maps for all the LDR images. Details in the brightest and darkest regions of the HDR scene are preserved better by the proposed algorithm without relative brightness change in the fused image...
January 16, 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28091802/increased-risk-of-revision-reoperation-and-implant-constraint-in-tka-after-multiligament-knee-surgery
#6
Steven I Pancio, Paul L Sousa, Aaron J Krych, Matthew P Abdel, Bruce A Levy, Diane L Dahm, Michael J Stuart
BACKGROUND: The risk of major complications and revision arthroplasty after TKA in patients who previously underwent multiligament knee surgery have been poorly characterized. QUESTIONS/PURPOSES: Is multiligament knee surgery before TKA associated with (1) worse implant survival, (2) increased use of TKA design constraint, (3) a greater risk for major complications, and (4) poorer scores for pain and function compared with similar patients receiving TKA for primary osteoarthritis? METHODS: Fifty-nine TKAs were performed at our institution between 1985 and 2014 in 59 patients (36 men, 23 women; mean age, 53 years) with a history of previous multiligament knee surgery (≥ two ligaments)...
January 13, 2017: Clinical Orthopaedics and related Research
https://www.readbyqxmd.com/read/28078360/multimodal-nerve-monitoring-during-periacetabular-osteotomy-identifies-surgical-steps-associated-with-risk-of-injury
#7
Eduardo N Novais, Travis Heare, Lauryn Kestel, Patricia Oliver, Willy Boucharel, Jason Koerner, Kim Strupp
PURPOSE: Sciatic nerve palsy after periacetabular osteotomy (PAO) is a serious complication. The purpose of this study was to determine whether a multimodal sciatic monitoring technique allows for identification of surgical steps that place the sciatic nerve at risk. METHODS: Transcranial electrical motor evoked potentials (TcMEPs), somatosensory evoked potentials (SSEPs), and spontaneous electromyography (EMG) were monitored in a consecutive series of 34 patients (40 hips) who underwent PAO for the treatment of symptomatic hip dysplasia between January 2012 and November 2014...
January 11, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28075551/cucurbit-n-uril-based-microcapsules-self-assembled-within-microfluidic-droplets-a-versatile-approach-for-supramolecular-architectures-and-materials
#8
Ji Liu, Yang Lan, Ziyi Yu, Cindy S Y Tan, Richard M Parker, Chris Abell, Oren A Scherman
Microencapsulation is a fundamental concept behind a wide range of daily applications ranging from paints, adhesives, and pesticides to targeted drug delivery, transport of vaccines, and self-healing concretes. The beauty of microfluidics to generate microcapsules arises from the capability of fabricating monodisperse and micrometer-scale droplets, which can lead to microcapsules/particles with fine-tuned control over size, shape, and hierarchical structure, as well as high reproducibility, efficient material usage, and high-throughput manipulation...
January 11, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28074783/automated-extraction-of-single-h-atoms-with-stm-tip-state-dependency
#9
Morten Møller, Samuel P Jarvis, Laurent Guérinet, Peter Sharp, Richard Woolley, Philipp Rahe, Philip Moriarty
The atomistic structure of the tip apex plays a crucial role in performing reliable atomic-scale surface and adsorbate manipulation using scanning probe techniques. We have developed an automated extraction routine for controlled removal of single hydrogen atoms from the H:Si(100) surface. The set of atomic extraction protocols detect a variety of desorption events during scanning tunneling microscope (STM)-induced modification of the hydrogen-passivated surface. The influence of the tip state on the probability for hydrogen removal was examined by comparing the desorption efficiency for various classifications of STM topographs (rows, dimers, atoms, etc)...
January 11, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28071925/gene-editing-and-genetic-engineering-approaches-for-advanced-probiotics-a-review
#10
Ruby Yadav, Vishal Kumar, Mehak Baweja, Pratyoosh Shukla
The applications of probiotics are significant and thus resulted in need of genome analysis of probiotic strains. Various omics methods and systems biology approaches enables us to understand and optimize the metabolic processes. These techniques have increased the researcher's attention towards gut microbiome and provided a new source for the revelation of uncharacterized biosynthetic pathways which enables novel metabolic engineering approaches. In recent years, the broad and quantitative analysis of modified strains relies on systems biology tools such as in silico design which are commonly used methods for improving strain performance...
January 10, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/28069780/a-chlorophyll-fluorescence-based-method-for-the-integrated-characterization-of-the-photophysiological-response-to-light-stress
#11
João Serôdio, William Schmidt, Silja Frankenbach
This work introduces a new experimental method for the comprehensive description of the physiological responses to light of photosynthetic organisms. It allows the integration in a single experiment of the main established manipulative chlorophyll fluorescence-based protocols. It enables the integrated characterization of the photophysiology of samples regarding photoacclimation state (generating non-sequential light-response curves of effective PSII quantum yield, electron transport rate or non-photochemical quenching), photoprotection capacity (running light stress-recovery experiments, quantifying non-photochemical quenching components) and the operation of photoinactivation and photorepair processes (measuring rate constants of photoinactivation and repair for different light levels and the relative quantum yield of photoinactivation)...
January 9, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28068055/development-of-advanced-dressings-for-the-delivery-of-progenitor-cells
#12
Giles T S Kirby, Stuart J Mills, Liesbeth Vandenpoel, Jef Pinxteren, Anthony Ting, Robert D Short, Allison J Cowin, Andrew Michelmore, Louise E Smith
Culture surfaces that substantially reduce the degree of cell manipulation in the delivery of cell sheets to patients are described. These surfaces support the attachment, culture and delivery of multipotent adult progenitor cells (MAPC). It was essential that the processes of attachment/detachment to the surface did not affect cell phenotype, nor the function of the cultured cells. Both acid-based and amine-based surface coatings were generated from acrylic acid, propanoic acid, diaminopropane and heptylamine precursors respectively...
January 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28062046/recent-advances-in-biological-single-molecule-applications-of-optical-tweezers-and-fluorescence-microscopy
#13
M Hashemi Shabestari, A E C Meijering, W H Roos, G J L Wuite, E J G Peterman
Over the past two decades, single-molecule techniques have evolved into robust tools to study many fundamental biological processes. The combination of optical tweezers with fluorescence microscopy and microfluidics provides a powerful single-molecule manipulation and visualization technique that has found widespread application in biology. In this combined approach, the spatial (~nm) and temporal (~ms) resolution, as well as the force scale (~pN) accessible to optical tweezers is complemented with the power of fluorescence microscopy...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28062045/single-molecule-optical-trapping-techniques-to-study-molecular-mechanisms-of-a-replisome
#14
B Sun, M D Wang
The replisome is a multiprotein molecular machinery responsible for the replication of DNA. It is composed of several specialized proteins each with dedicated enzymatic activities, and in particular, helicase unwinds double-stranded DNA and DNA polymerase catalyzes the synthesis of DNA. Understanding how a replisome functions in the process of DNA replication requires methods to dissect the mechanisms of individual proteins and of multiproteins acting in concert. Single-molecule optical-trapping techniques have proved to be a powerful approach, offering the unique ability to observe and manipulate biomolecules at the single-molecule level and providing insights into the mechanisms of molecular motors and their interactions and coordination in a complex...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28062034/integrating-optical-tweezers-dna-tightropes-and-single-molecule-fluorescence-imaging-pitfalls-and-traps
#15
J Wang, J T Barnett, M R Pollard, N M Kad
Fluorescence imaging is one of the cornerstone techniques for understanding how single molecules search for their targets on DNA. By tagging individual proteins, it is possible to track their position with high accuracy. However, to understand how proteins search for targets, it is necessary to elongate the DNA to avoid protein localization ambiguities. Such structures known as "DNA tightropes" are tremendously powerful for imaging target location; however, they lack information about how force and load affect protein behavior...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28062033/high-resolution-optical-tweezers-combined-with-single-molecule-confocal-microscopy
#16
K D Whitley, M J Comstock, Y R Chemla
We describe the design, construction, and application of an instrument combining dual-trap, high-resolution optical tweezers and a confocal microscope. This hybrid instrument allows nanomechanical manipulation and measurement simultaneously with single-molecule fluorescence detection. We present the general design principles that overcome the challenges of maximizing optical trap resolution while maintaining single-molecule fluorescence sensitivity, and provide details on the construction and alignment of the instrument...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28060422/identification-of-fungi-isolated-from-banana-rachis-and-characterization-of-their-surface-activity
#17
Lina Mendez-Castillo, Erlide Prieto-Correa, Carlos Jimenez-Junca
Filamentous fungi are an unexplored source for the production of biosurfactants, but over a decade it was discovered one of the most surface active molecules called hydrophobins. There are few techniques to determine the surface activity of fungi without some kind of manipulation that can affect the final results. In this work, we identified 33 strains of filamentous fungi isolated from banana rachis which may have potential in producing biosurfactants. Further the production of surface active compounds by the strains was measured by two techniques...
January 6, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28060343/protein-complex-affinity-capture-from-cryomilled-mammalian-cells
#18
John LaCava, Hua Jiang, Michael P Rout
Affinity capture is an effective technique for isolating endogenous protein complexes for further study. When used in conjunction with an antibody, this technique is also frequently referred to as immunoprecipitation. Affinity capture can be applied in a bench-scale and in a high-throughput context. When coupled with protein mass spectrometry, affinity capture has proven to be a workhorse of interactome analysis. Although there are potentially many ways to execute the numerous steps involved, the following protocols implement our favored methods...
December 9, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28060262/sub-nanometer-resolution-imaging-with-amplitude-modulation-atomic-force-microscopy-in-liquid
#19
Ethan J Miller, William Trewby, Amir Farokh Payam, Luca Piantanida, Clodomiro Cafolla, Kislon Voïtchovsky
Atomic force microscopy (AFM) has become a well-established technique for nanoscale imaging of samples in air and in liquid. Recent studies have shown that when operated in amplitude-modulation (tapping) mode, atomic or molecular-level resolution images can be achieved over a wide range of soft and hard samples in liquid. In these situations, small oscillation amplitudes (SAM-AFM) enhance the resolution by exploiting the solvated liquid at the surface of the sample. Although the technique has been successfully applied across fields as diverse as materials science, biology and biophysics and surface chemistry, obtaining high-resolution images in liquid can still remain challenging for novice users...
December 20, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28060258/insect-controlled-robot-a-mobile-robot-platform-to-evaluate-the-odor-tracking-capability-of-an-insect
#20
Noriyasu Ando, Shuhei Emoto, Ryohei Kanzaki
Robotic odor source localization has been a challenging area and one to which biological knowledge has been expected to contribute, as finding odor sources is an essential task for organism survival. Insects are well-studied organisms with regard to odor tracking, and their behavioral strategies have been applied to mobile robots for evaluation. This "bottom-up" approach is a fundamental way to develop biomimetic robots; however, the biological analyses and the modeling of behavioral mechanisms are still ongoing...
December 19, 2016: Journal of Visualized Experiments: JoVE
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