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https://www.readbyqxmd.com/read/29028017/arbitrary-shaped-brillouin-microwave-photonic-filter-by-manipulating-a-directly-modulated-pump
#1
Wei Wei, Lilin Yi, Yves Jaouën, Weisheng Hu
We present a cost-effective gigahertz-wide arbitrary-shaped microwave photonic filter based on stimulated Brillouin scattering in fiber using a directly modulated laser (DML). After analyzing the relationship between the spectral power density and the modulation current of the DML, we manage to precisely adjust the optical spectrum of the DML, thereby controlling the Brillouin filter response arbitrarily for the first time, to the best of our knowledge. The filter performance is evaluated by amplifying a 500 Mb/s non-return-to-zero on-off keying signal using a 1 GHz rectangular filter...
October 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/29027787/detection-of-t4-polynucleotide-kinase-via-allosteric-aptamer-probe-platform
#2
Mingxuan Gao, Jingjing Guo, Yanling Song, Zhi Zhu, Chaoyong James Yang
As a vital enzyme in DNA phosphorylation and restoration, T4 polynuceotide kinase (T4 PNK) has aroused great interest in recent years. Numerous strategies have been established for highly sensitive detection of T4 PNK based on diverse signal amplification techniques. However, they often need sophisticated design, a variety of auxiliary reagents and enzymes, or cumbersome manipulations. Herein, we have designed a new kind of allosteric aptamer probe consisting of streptavidin aptamer (SA aptamer) and the complementary DNA (cDNA) for simple detection of T4 PNK without signal amplification and with minimized interference in complex biological samples...
October 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29027186/novel-technique-for-intraoperative-sciatic-nerve-assessment-in-complex-primary-total-hip-arthroplasty-a-pilot-study
#3
Shai S Shemesh, Jonathan Robinson, Samuel Overley, Michael J Bronson, Calin S Moucha, Darwin Chen
INTRODUCTION: Sciatic nerve injury (SNI) is a potentially devastating complication after total hip arthroplasty (THA). Intraoperative neural monitoring has been found in several studies to be useful in preventing SNI, but can be difficult to implement. In this study, we examine the results of using a handheld nerve stimulator for intraoperative sciatic nerve (SN) monitoring during complex THA requiring limb lengthening and/or significant manipulation of the SN. METHODS: A consecutive series of 11 cases (9 patients, 11 hips) with either severe developmental dysplasia of the hip (Crowe 3-4) or other underlying conditions requiring complex hip reconstruction involving significant leg lengthening and/or nerve manipulation...
October 9, 2017: Hip International: the Journal of Clinical and Experimental Research on Hip Pathology and Therapy
https://www.readbyqxmd.com/read/29027094/multimodal-functional-neuroimaging-by-simultaneous-bold-fmri-and-fiber-optic-calcium-recordings-and-optogenetic-control
#4
REVIEW
Franziska Albers, Lydia Wachsmuth, Timo Mauritz van Alst, Cornelius Faber
Recent developments of optogenetic tools and fluorescence-based calcium recording techniques enable the manipulation and monitoring of neural circuits on a cellular level. Non-invasive imaging of brain networks, however, requires the application of methods such as blood oxygen level-dependent (BOLD) functional magnetic resonance imaging (fMRI), which is commonly used for functional neuroimaging. While BOLD fMRI provides brain-wide non-invasive reading of the hemodynamic response, it is only an indirect measure of neural activity...
October 12, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/29020962/manipulation-under-anesthesia-versus-physiotherapy-treatment-in-stage-two-of-a-frozen-shoulder-a-study-protocol-for-a-randomized-controlled-trial
#5
Tim Kraal, Bertram The, Ronald Boer, M P van den Borne, Koen Koenraadt, Pjotr Goossens, Denise Eygendaal
BACKGROUND: There is no consensus about the optimal treatment strategy for frozen shoulders (FS). Conservative treatment consisting of intra-articular corticosteroid infiltrations and physiotherapy are considered appropriate for most patients. However, with a conservative strategy, patients experience a prolonged rehabilitation period with a considerable amount of pain and disabilities in daily life. Also, at long term, a residual amount of pain and restriction of range of motion is frequently reported...
October 11, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/29018456/virtual-plants-need-water-too-functional-structural-root-system-models-in-the-context-of-drought-tolerance-breeding
#6
REVIEW
Adama Ndour, Vincent Vadez, Christophe Pradal, Mikaël Lucas
Developing a sustainable agricultural model is one of the great challenges of the coming years. The agricultural practices inherited from the Green Revolution of the 1960s show their limits today, and new paradigms need to be explored to counter rising issues such as the multiplication of climate-change related drought episodes. Two such new paradigms are the use of functional-structural plant models to complement and rationalize breeding approaches and a renewed focus on root systems as untapped sources of plant amelioration...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28994798/large-volume-behaviorally-relevant-illumination-for-optogenetics-in-non-human-primates
#7
Leah C Acker, Erica N Pino, Edward S Boyden, Robert Desimone
This protocol describes a large-volume illuminator, which was developed for optogenetic manipulations in the non-human primate brain. The illuminator is a modified plastic optical fiber with etched tip, such that the light emitting surface area is > 100x that of a conventional fiber. In addition to describing the construction of the large-volume illuminator, this protocol details the quality-control calibration used to ensure even light distribution. Further, this protocol describes techniques for inserting and removing the large volume illuminator...
October 3, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28994781/methods-for-imaging-intracellular-ph-of-the-follicle-stem-cell-lineage-in-live-drosophila-ovarian-tissue
#8
Sumitra Tatapudy, Marimar Benitez, Todd Nystul
Changes in intracellular pH (pHi) play important roles in the regulation of many cellular functions, including metabolism, proliferation, and differentiation. Typically, pHi dynamics are determined in cultured cells, which are amenable to measuring and experimentally manipulating pHi. However, the recent development of new tools and methodologies has made it possible to study pHi dynamics within intact, live tissue. For Drosophila research, one important development was the generation of a transgenic line carrying a pHi biosensor, mCherry::pHluorin...
September 26, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28990995/donor-tissue-preparation-for-descemet-membrane-endothelial-keratoplasty-an-updated-review
#9
Rénuka S Birbal, Shameema Sikder, Jessica T Lie, Esther A Groeneveld-van Beek, Silke Oellerich, Gerrit R J Melles
PURPOSE: To provide an overview of the current literature on donor tissue preparation for Descemet membrane endothelial keratoplasty (DMEK). METHODS: A comprehensive database search without date restrictions was performed in PubMed and in The Cochrane Library in May, 2017. Keywords included Descemet membrane endothelial keratoplasty, corneal transplantation, graft, harvest, dissection, preparation, endothelial cell, and endothelial cell density. Articles aiming to describe or evaluate a technique for DMEK graft preparation were considered eligible and were included in this review...
October 5, 2017: Cornea
https://www.readbyqxmd.com/read/28990676/arthroscopic-approach-and-anatomy-of-the-stifle-joint-in-the-rabbit
#10
Sarah Torrey Peters, Markus Wilke, Tanja Schmid
OBJECTIVE: To describe a technique and normal findings for arthroscopy of the stifle in rabbits. STUDY DESIGN: Cadaver study. ANIMALS: Twenty cadaveric stifles from New Zealand White rabbits were examined. METHODS: The arthroscope was placed through a medial portal, and instrument portals were created on the lateral aspect of the joint. The same portals were used for examination of the entire joint and palpation of structures with a probe...
October 9, 2017: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/28990614/microfluidic-magnetic-bead-conveyor-belt
#11
Stijn van Pelt, Arjan Frijns, Jaap den Toonder
Magnetic beads play an important role in the miniaturization of clinical diagnostics systems. In lab-on-chip platforms, beads can be made to link to a target species and can then be used for the manipulation and detection of this species. Current bead actuation systems utilize complex on-chip coil systems that offer low field strengths and little versatility. We demonstrate a novel system based on an external rotating magnetic field and on-chip soft-magnetic structures to focus the field locally. These structures were designed and optimized using finite element simulations in order to create a number of local flux density maxima...
October 9, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28986519/crispr-cas9-induced-transgene-insertion-and-telomere-associated-truncation-of-a-single-human-chromosome-for-chromosome-engineering-in-cho-and-a9-cells
#12
Narumi Uno, Kei Hiramatsu, Katsuhiro Uno, Shinya Komoto, Yasuhiro Kazuki, Mitsuo Oshimura
Chromosome engineering techniques including gene insertion, telomere-associated truncation and microcell-mediated chromosome transfer (MMCT) are powerful tools for generation of humanised model animal, containing megabase-sized genomic fragments. However, these techniques require two cell lines: homologous recombination (HR)-proficient DT40 cells for chromosome modification, and CHO cells for transfer to recipient cells. Here we show an improved technique using a combination of CRISPR/Cas9-induced HR in CHO and mouse A9 cells without DT40 cells following MMCT to recipient cells...
October 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28985331/an-introductory-how-to-guide-for-incorporating-microbiome-research-into-integrative-and-comparative-biology
#13
Kevin D Kohl
Research on host-associated microbial communities has grown rapidly. Despite the great body of work, inclusion of microbiota-related questions into integrative and comparative biology is still lagging behind other disciplines. The purpose of this paper is to offer an introduction into the basic tools and techniques of host-microbe research. Specifically, what considerations should be made before embarking on such projects (types of samples, types of controls)? How is microbiome data analyzed and integrated with data measured from the hosts? How can researchers experimentally manipulate the microbiome? With this information, integrative and comparative biologists should be able to include host-microbe studies into their research and push the boundaries of both fields...
July 25, 2017: Integrative and Comparative Biology
https://www.readbyqxmd.com/read/28984630/perioperative-management-of-the-adult-patient-on-venovenous-extracorporeal-membrane-oxygenation-requiring-noncardiac-surgery
#14
Michael A Fierro, Mani A Daneshmand, Raquel R Bartz
The use of venovenous extracorporeal membrane oxygenation is increasing worldwide. These patients often require noncardiac surgery. In the perioperative period, preoperative assessment, patient transport, choice of anesthetic type, drug dosing, patient monitoring, and intraoperative and postoperative management of common patient problems will be impacted. Furthermore, common monitoring techniques will have unique limitations. Importantly, patients on venovenous extracorporeal membrane oxygenation remain subject to hypoxemia, hypercarbia, and acidemia in the perioperative setting despite extracorporeal support...
October 4, 2017: Anesthesiology
https://www.readbyqxmd.com/read/28984511/interbody-distraction-and-vertebral-body-reconstruction-with-polymethylmethacrylate-for-the-treatment-of-pathological-fractures
#15
Scott L Zuckerman, Ganesh Rao, Laurence D Rhines, Ian E McCutcheon, Richard G Everson, Claudio E Tatsui
OBJECTIVE Treatment of epidural spinal cord compression (ESCC) caused by tumor includes surgical decompression and stabilization followed by postoperative radiation. In the case of severe axial loading impairment, anterior column reconstruction is indicated. The authors describe the use of interbody distraction to restore vertebral body height and correct kyphotic angulation prior to reconstruction with polymethylmethacrylate (PMMA), and report the long-term durability of such reconstruction. METHODS A single institution, prospective series of patients with ESCC undergoing single-stage decompression, anterior column reconstruction, and posterior instrumentation from 2013 to 2016 was retrospectively analyzed...
October 6, 2017: Journal of Neurosurgery. Spine
https://www.readbyqxmd.com/read/28980237/avian-biotechnology
#16
Yoshiaki Nakamura
Primordial germ cells (PGCs) generate new individuals through differentiation, maturation and fertilization. This means that the manipulation of PGCs is directly linked to the manipulation of individuals, making PGCs attractive target cells in the animal biotechnology field. A unique biological property of avian PGCs is that they circulate temporarily in the vasculature during early development, and this allows us to access and manipulate avian germ lines. Following the development of a technique for transplantation, PGCs have become central to avian biotechnology, in contrast to the use of embryo manipulation and subsequent transfer to foster mothers, as in mammalian biotechnology...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28975645/effects-of-electrothermal-vortices-on-insulator-based-dielectrophoresis-for-circulating-tumor-cell-separation
#17
Arian Aghilinejad, Mohammad Aghaamoo, Xiaolin Chen, Jie Xu
Insulator-based dielectrophoresis (iDEP) is known as a powerful technique for separation and manipulation of bioparticles. In recent years, iDEP designs using arrays of insulating posts have shown promising results towards reaching high-efficiency bioparticle manipulation. Joule heating (JH) and electrothermal (ET) flows have been observed in iDEP microdevices and significantly affecting their performances. In this research, we utilize mathematical modeling to study, in detail, iDEP technique and the effects of JH and ET flow on device performance and propose our separation scenario for selective trapping of circulating tumor cells (CTCs)...
October 4, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28975547/autologous-and-micro-fragmented-adipose-tissue-for-the-treatment-of-diffuse-degenerative-knee-osteoarthritis
#18
A Russo, V Condello, V Madonna, M Guerriero, C Zorzi
BACKGROUND: Chondral lesions of the knee represent a challenge for the orthopaedic surgeon. Several treatments have been proposed with variable success rate. Recently, new therapeutic approaches, such as the use of mesenchymal stem cells, have shown promising results. The adipose tissue is a good source of these naturally occurring regenerative cells, due to its abundance and easy access. In addition, it can be used to provide cushioning and filling of structural defects. The 1-year safety and outcome of a single intra-articular injection of autologous and micro-fragmented adipose tissue in 30 patients affected by diffuse degenerative chondral lesions was evaluated...
October 3, 2017: Journal of Experimental Orthopaedics
https://www.readbyqxmd.com/read/28973769/periclinal-chimera-technique-new-plant-breeding-approach
#19
P M Gakpetor, H Mohammed, D Moreti, N M A Nassar
Plant interspecific periclinal chimeras are a mosaic formed by tissues from two species. They are manipulated here as an efficient plant breeding tool for cassava root yields. In this study, plants synthesized from two chimeras, designated as chimera 2 and chimera 4, were characterized morphologically and cytologically to unravel the origin of their tissue layers (L2 and L3). Root yield of the two chimeras was also evaluated. Chimera 2 that was developed from graft union between Manihot fortalezensis (F) as scion and M...
September 21, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28973693/endoscopic-endonasal-and-transcranial-surgery-for-microsurgical-resection-of-ventral-foramen-magnum-meningiomas-a-preliminary-experience
#20
Nicolas Khattar, Maria Koutourousiou, Joseph D Chabot, Eric W Wang, Aaron A Cohen-Gadol, Carl H Snyderman, Juan C Fernandez-Miranda, Paul A Gardner
BACKGROUND: Purely ventral foramen magnum meningiomas are challenging tumors to treat given their location, and proximity and relationship to vital neurovascular structures. OBJECTIVE: To present endoscopic endonasal surgery (EES) as a complementary approach to the far-lateral suboccipital approach (FLA) for ventral midline tumors. METHODS: From May 2008 to October 2013, 5 patients underwent EES and 5 FLA for primary ventral foramen magnum meningiomas...
July 22, 2017: Operative Neurosurgery (Hagerstown, Md.)
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