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https://www.readbyqxmd.com/read/28334274/interactive-effects-of-multiple-climate-change-factors-on-ammonia-oxidizers-and-denitrifiers-in-a-temperate-steppe
#1
Cuijing Zhang, Jupei Shen, Yifei Sun, Juntao Wang, Limei Zhang, Zhongling Yang, Hongyan Han, Shiqiang Wan, Jizheng He
Global climate change could have profound effects on belowground microbial communities and subsequently affect soil biogeochemical processes. The interactive effects of multiple co-occurring climate change factors on microbially-mediated processes are not well understood. A four-factorial field experiment with elevated CO2, watering, nitrogen (N) addition and night warming was conducted in a temperate steppe of northern China. Real-time polymerase chain reaction and terminal-restriction fragment length polymorphism, combined with clone library techniques were applied to examine the effects of those climate change factors on N-related microbial abundance and community composition...
March 15, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28333031/new-approaches-regarding-the-in-vitro-maturation-of-oocytes-manipulating-cyclic-nucleotides-and-their-partners-in-crime
#2
REVIEW
Ramon Cesar Botigelli, Eduardo Montanari Razza, Elisa Mariano Pioltine, Marcelo Fábio Gouveia Nogueira
Several discoveries have been described recently (5-10 years) about the biology of ovarian follicles (oocyte, cumulus cells and granulosa cells), including new aspects of cellular communication, the control of oocyte maturation and the acquisition of oocyte competence for fertilization and further embryo development. These advances are nourishing assisted reproduction techniques (ART) with new possibilities, in which novel culture systems are being developed and tested to improve embryo yield and quality. This mini-review aims to describe how the recent knowledge on the physiological aspects of mammalian oocyte is reflecting as original or revisited approaches into the context of embryo production...
February 1, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28331312/investigation-of-silk-fibroin-nanoparticle-decorated-poly-l-lactic-acid-composite-scaffolds-for-osteoblast-growth-and-differentiation
#3
Biao-Qi Chen, Ranjith Kumar Kankala, Ai-Zheng Chen, Ding-Zhu Yang, Xiao-Xia Cheng, Ni-Na Jiang, Kai Zhu, Shi-Bin Wang
Attempts to reflect the physiology of organs is quite an intricacy during the tissue engineering process. An ideal scaffold and its surface topography can address and manipulate the cell behavior during the regeneration of targeted tissue, affecting the cell growth and differentiation significantly. Herein, silk fibroin (SF) nanoparticles were incorporated into poly(l-lactic acid) (PLLA) to prepare composite scaffolds via phase-inversion technique using supercritical carbon dioxide (SC-CO2). The SF nanoparticle core increased the surface roughness and hydrophilicity of the PLLA scaffolds, leading to a high affinity for albumin attachment...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28327731/scattering-properties-and-internal-structure-of-magnetic-filament-brushes
#4
Elena S Pyanzina, Pedro A Sánchez, Joan J Cerdà, Tomàs Sintes, Sofia S Kantorovich
Practical applications of polymer brush-like systems rely on a clear understanding of their internal structure. In the case of magnetic nanoparticle filament brushes, the competition between bonding and nonbonding interactions-including long range magnetic dipole-dipole interactions-makes the microstructure of these polymer brush-like systems rather complex. On the other hand, the same interactions open up the possibility to manipulate the meso- and macroscopic responses of these systems by applying external magnetic fields or by changing the background temperature...
March 22, 2017: Soft Matter
https://www.readbyqxmd.com/read/28326567/both-standing-and-postural-threat-decrease-achilles-tendon-reflex-inhibition-from-tendon-electrical-stimulation
#5
Brian C Horslen, J Timothy Inglis, Jean-Sébastien Blouin, Mark G Carpenter
Golgi tendon organ Ib reflexes are thought to contribute to standing balance control, but it is unknown if they are modulated when people are exposed to a postural threat. We used a novel application of tendon electrical stimulation (TStim) to elicit Ib inhibitory reflexes in the medial gastrocnemius, while actively engaged in upright standing balance, to examine a) how Ib reflexes to TStim are influenced by upright stance, and b) the effects of height-induced postural threat on Ib reflexes during standing...
March 22, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28324822/outcome-evaluation-of-minimally-invasive-surgery-versus-extensile-lateral-approach-in-management-of-displaced-intra-articular-calcaneal-fractures-a-randomised-control-trial
#6
Ankit Khurana, Mandeep S Dhillon, Sharad Prabhakar, Rakesh John
BACKGROUND: Minimally invasive surgery has a significant and evolving role in the treatment of DIACFs, but there is limited literature on this topic. OBJECTIVES: To compare the clinico-radiological outcomes of DIACFs fixed with MIS technique with ORIF. METHODS: This randomised control trial (RCT) included 21 closed Sanders type 2 and 3 DIACFs which were selected from 70 who presented. Extensile lateral approach was used in the ORIF group; while MIS techniques included either percutaneous reduction or small incisions with indirect fragment manipulation...
February 20, 2017: Foot
https://www.readbyqxmd.com/read/28324650/genome-engineering-of-virulent-lactococcal-phages-using-crispr-cas9
#7
Marie-Laurence Lemay, Denise M Tremblay, Sylvain Moineau
Phages are biological entities found in every ecosystem. Although much has been learned about them in past decades, significant knowledge gaps remain. Manipulating virulent phage genomes is challenging. To date, no efficient gene-editing tools exist for engineering virulent lactococcal phages. Lactococcus lactis is a bacterium extensively used as a starter culture in various milk fermentation processes and its phage sensitivity poses a constant risk to the cheese industry. The lactococcal phage p2 is one of the best-studied models for these virulent phages...
March 21, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28324213/-split-and-splayed-c3-traumatic-lateral-c2-3-dislocation-without-neurological-deficits-unique-case-and-its-management
#8
Apinderpreeet Singh, Pravin Salunke, Rajeev P Kamble
BACKGROUND: Traumatic C2-3 dislocation has been often described in the antero-posterior plane but is extremely rare in the lateral plane. Such dislocations have been described in thoraco-lumbar and C1-2. The need to study the imaging in multiple planes has been highlighted to plan proper realignment. METHODS: A young male presented with neck pain alone following a train accident. The imaging showed a fracture of C3 splaying it. The C2-3 showed lateral dislocation in the lateral plane with locked C2-3 facets on one side and C3-4 facets on the other...
March 21, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28320656/targeting-effects-on-the-volume-of-the-focused-ultrasound-induced-blood-brain-barrier-opening-in-non-human-primates-in-vivo
#9
Maria Eleni Karakatsani, Gesthimani Samiotaki, Matthew Downs, Vincent Ferrera, Elisa Konofagou
Drug delivery to subcortical regions is susceptible to the blood-brain barrier (BBB) impeding the molecular exchange between the blood stream and the brain parenchyma. Focused ultrasound coupled with the administration of microbubbles has been proven to open the BBB locally, transiently and non-invasively both in rodents and in Non-Human-Primates (NHPs). The development of this disruption technique independent of MRI monitoring is of primordial importance yet restrained to the targeting optimization. The current paper establishes the linear relationship of the incidence angle with the volume of BBB opening (VBBB) and the Peak Negative Pressure (PNP) when sonicating the Caudate Nucleus and the Putamen region of five non-human-primates...
March 13, 2017: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://www.readbyqxmd.com/read/28318292/from-play-to-proficiency-the-ontogeny-of-stone-tool-use-in-coastal-foraging-long-tailed-macaques-macaca-fascicularis-from-a-comparative-perception-action-perspective
#10
Amanda W Y Tan
Macaques crack shellfish in coastal environments with specialized stone-hammering techniques. I provide the first examination of skill development from 866 object-manipulation and 7,400 tool-use bouts, collected over 15 months, using longitudinal analyses of infants' object manipulation (N = 7) and cross-sectional comparisons of manipulative and tool-use behavior (N = 69). I adopt a Perception-action approach, examining how the emergence of actions on objects relate to the spatial-relational and percussive challenges of tool use...
March 20, 2017: Journal of Comparative Psychology
https://www.readbyqxmd.com/read/28317860/gene-delivery-to-nile-tilapia-cells-for-transgenesis-and-the-role-of-pi3k-c2%C3%AE-in-angiogenesis
#11
Fernanda Maria Policarpo Tonelli, Samyra Maria Dos Santos Nassif Lacerda, Marcela Santos Procópio, Breno Luiz Sales Lemos, Luiz Renato de França, Rodrigo Ribeiro Resende
Microinjection is commonly performed to achieve fish transgenesis; however, due to difficulties associated with this technique, new strategies are being developed. Here we evaluate the potential of lentiviral particles to genetically modify Nile tilapia cells to achieve transgenesis using three different approaches: spermatogonial stem cell (SSC) genetic modification and transplantation (SC), in vivo transduction of gametes (GT), and fertilised egg transduction (ET). The SC protocol using larvae generates animals with sustained production of modified sperm (80% of animals with 77% maximum sperm fluorescence [MSF]), but is a time-consuming protocol (sexual maturity in Nile tilapia is achieved at 6 months of age)...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28314822/topography-and-lateralized-effect-of-acute-aromatase-inhibition-on-auditory-processing-in-a-seasonal-songbird
#12
Geert De Groof, Jacques Balthazart, Charlotte A Cornil, Annemie Van der Lindena
It is increasingly recognized that brain-derived estrogens (neuroestrogens) can regulate brain physiology and behavior much faster than what was previously known from the transcriptional action of estrogens on nuclear receptors. One of the best examples of such neuromodulation by neuroestrogens concerns the acute regulation of sensory coding by the auditory cortex as demonstrated by electrophysiological studies of selected neurons in zebra finches. Yet, the spatial extent of such modulation by neuroestrogens is not known...
March 17, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28314520/interaction-between-postural-asymmetry-and-visual-feedback-effects-in-undisturbed-upright-stance-control-in-healthy-adults
#13
Patrice R Rougier, Samir Boudrahem
OBJECTIVES: The technique of additional visual feedback has been shown to significantly decrease the center of pressure (CP) displacements of a standing subject. Body-weight asymmetry is known to increase postural instability due to difficulties in coordinating the reaction forces exerted under each foot and is often a cardinal feature of various neurological and traumatic diseases. To examine the possible interactions between additional visual feedback and body-weight asymmetry effects, healthy adults were recruited in a protocol with and without additional visual feedback, with different levels of body-weight asymmetry...
March 14, 2017: Neurophysiologie Clinique, Clinical Neurophysiology
https://www.readbyqxmd.com/read/28306293/particle-hole-asymmetry-in-the-cuprate-pseudogap-measured-with-time-resolved-spectroscopy
#14
Tristan L Miller, Wentao Zhang, Hiroshi Eisaki, Alessandra Lanzara
One of the most puzzling features of high-temperature cuprate superconductors is the pseudogap state, which appears above the temperature at which superconductivity is destroyed. There remain fundamental questions regarding its nature and its relation to superconductivity. But to address these questions, we must first determine whether the pseudogap and superconducting states share a common property: particle-hole symmetry. We introduce a new technique to test particle-hole symmetry by using laser pulses to manipulate and measure the chemical potential on picosecond time scales...
March 3, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28306161/dna-dielectrophoresis-theory-and-applications-a-review
#15
REVIEW
Martina Viefhues, Ralf Eichhorn
Dielectrophoresis is the migration of an electrically polarizable particle in an inhomogeneous electric field. This migration can be exploited for several applications with (bio)molecules or cells. Dielectrophoresis is a non-invasive technique; therefore, it is very convenient for (selective) manipulation of (bio)molecules or cells. In this review, we will focus on DNA dielectrophoresis as this technique offers several advantages in trapping and immobilization, separation and purification, and analysis of DNA molecules...
March 17, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28304064/surface-point-defects-on-bulk-oxides-atomically-resolved-scanning-probe-microscopy
#16
Martin Setvín, Margareta Wagner, Michael Schmid, Gareth S Parkinson, Ulrike Diebold
Metal oxides are abundant in nature and they are some of the most versatile materials for applications ranging from catalysis to novel electronics. The physical and chemical properties of metal oxides are dramatically influenced, and can be judiciously tailored, by defects. Small changes in stoichiometry introduce so-called intrinsic defects, e.g., atomic vacancies and/or interstitials. This review gives an overview of using Scanning Probe Microscopy (SPM), in particular Scanning Tunneling Microscopy (STM), to study the changes in the local geometric and electronic structure related to these intrinsic point defects at the surfaces of metal oxides...
March 17, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28303914/restoring-brain-function-after-stroke-bridging-the-gap-between-animals-and-humans
#17
REVIEW
Nick S Ward
Stroke is the leading cause of complex adult disability in the world. Recovery from stroke is often incomplete, which leaves many people dependent on others for their care. The improvement of long-term outcomes should, therefore, be a clinical and research priority. As a result of advances in our understanding of the biological mechanisms involved in recovery and repair after stroke, therapeutic opportunities to promote recovery through manipulation of poststroke plasticity have never been greater. This work has almost exclusively been carried out in preclinical animal models of stroke with little translation into human studies...
March 17, 2017: Nature Reviews. Neurology
https://www.readbyqxmd.com/read/28303882/hidden-amorphous-phase-and-reentrant-supercooled-liquid-in-pd-ni-p-metallic-glasses
#18
S Lan, Y Ren, X Y Wei, B Wang, E P Gilbert, T Shibayama, S Watanabe, M Ohnuma, X-L Wang
An anomaly in differential scanning calorimetry has been reported in a number of metallic glass materials in which a broad exothermal peak was observed between the glass and crystallization temperatures. The mystery surrounding this calorimetric anomaly is epitomized by four decades long studies of Pd-Ni-P metallic glasses, arguably the best glass-forming alloys. Here we show, using a suite of in situ experimental techniques, that Pd-Ni-P alloys have a hidden amorphous phase in the supercooled liquid region...
March 17, 2017: Nature Communications
https://www.readbyqxmd.com/read/28303648/subadventitial-techniques-for-chronic-total-occlusion-percutaneous-coronary-intervention-the-concept-of-vessel-architecture
#19
Lorenzo Azzalini, Mauro Carlino, Emmanouil S Brilakis, Minh Vo, Stéphane Rinfret, Barry F Uretsky, Dimitri Karmpaliotis, Antonio Colombo
Despite improvements in guidewire technologies, the traditional antegrade wire escalation approach to chronic total occlusion (CTO) recanalization is successful in only 60-80% of selected cases. In particular, long, calcified, and tortuous occlusions are less successfully approached with a true-to-true lumen approach. Frequently, the guidewire tracks into the subadventitial space, with no guarantee of distal re-entry into the true lumen. The ability to manage the subadventitial space has been a key step in the tremendous improvement in success rates of contemporary CTO percutaneous coronary intervention (PCI), whether operating antegradely or retrogradely...
March 17, 2017: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/28303610/new-rescue-technique-of-endoscopic-ultrasonography-guided-rendezvous-procedure-with-video
#20
Hiroshi Kawakami, Yoshimasa Kubota
The newly developed endoscopic ultrasonography-guided rendezvous procedure (EUS-RV) is used after failed endoscopic retrograde cholangiopancreatography (ERCP) [1,2]. Guidewire manipulation is one of the most challenging aspects of EUS-RV. A rescue technique that bounces back the guidewire from the perihilar bile duct in a long endoscopic position is commonly used. Herein, we describe a case using EUS-RV in a short endoscopic position. This article is protected by copyright. All rights reserved.
March 16, 2017: Digestive Endoscopy: Official Journal of the Japan Gastroenterological Endoscopy Society
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