keyword
MENU ▼
Read by QxMD icon Read
search

Sulfolobus

keyword
https://www.readbyqxmd.com/read/27932585/structural-and-functional-characterization-of-the-tyw3-taw3-class-of-sam-dependent-methyltransferases
#1
Mark A Currie, Greg Brown, Andrew Wong, Takayuki Ohira, Kei Sugiyama, Tsutomu Suzuki, Alexander F Yakunin, Zongchao Jia
S-adenosylmethionine (SAM)-dependent methyltransferases regulate a wide range of biological processes through the modification of proteins, nucleic acids, polysaccharides, as well as various metabolites. TYW3/Taw3 is a SAM-dependent methyltransferase responsible for the formation of a tRNA modification known as wybutosine and its derivatives that are required for accurate decoding in protein synthesis. Here, we report the crystal structure of Taw3, a homolog of TYW3 from Sulfolobus solfataricus, which revealed a novel α/β fold...
December 8, 2016: RNA
https://www.readbyqxmd.com/read/27908245/perfect-hemihedral-twinning-in-crystals-of-the-%C3%AE-subunit-of-translation-initiation-factor-2-from-sulfolobus-solfataricus-cause-and-effect
#2
O V Kravchenko, O S Nikonov, N A Nevskaya, E A Stolboushkina, V I Arkhipova, M B Garber, S V Nikonov
The crystal structure of the γ-subunit of translation initiation factor 2 from the archaeon Sulfolobus solfataricus (SsoIF2γ) has been solved based on perfectly hemihedral twinned data. The protein was cocrystallized with the 10-fold molar excess of GTP analog (GDPCP) over protein. However, no nucleotide was found in the structure, and the model demonstrated the apo form of the protein. Two slightly different molecules in the asymmetric unit of the crystal are related by the non-crystallographic 2-fold axis and form a tightly associated dimer...
October 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27907170/complementation-of-an-aglb-mutant-of-methanococcus-maripaludis-with-heterologous-oligosaccharyltransferases
#3
Yan Ding, Helen A Vrionis, James Schneider, Alison Berezuk, Cezar M Khursigara, Ken F Jarrell
The oligosaccharyltransferase is the signature enzyme for N-linked glycosylation in all domains of life. In Archaea, this enzyme termed AglB, is responsible for transferring lipid carrier-linked glycans to select asparagine residues in a variety of target proteins including archaellins, S-layer proteins and pilins. This study investigated the ability of a variety of AglBs to compensate for the oligosaccharyltransferase activity in Methanococcus maripaludis deleted for aglB, using archaellin FlaB2 as the reporter protein since all archaellins in Mc...
2016: PloS One
https://www.readbyqxmd.com/read/27892499/recognition-of-extremophilic-archaeal-viruses-by-eukaryotic-cells-a-promising-nanoplatform-from-the-third-domain-of-life
#4
Kristine Buch Uldahl, Linping Wu, Arnaldur Hall, Pavlos Papathanasiou, Xu Peng, Seyed Moein Moghimi
Viruses from the third domain of life, Archaea, exhibit unusual features including extreme stability that allow their survival in harsh environments. In addition, these species have never been reported to integrate into human or any other eukaryotic genomes, and could thus serve for exploration of novel medical nanoplatforms. Here, we selected two archaeal viruses Sulfolobus monocaudavirus 1 (SMV1) and Sulfolobus spindle shaped virus 2 (SSV2) owing to their unique spindle shape, hyperthermostable and acid-resistant nature and studied their interaction with mammalian cells...
November 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27882920/repression-of-rna-polymerase-by-the-archaeo-viral-regulator-orf145-rip
#5
Carol Sheppard, Fabian Blombach, Adam Belsom, Sarah Schulz, Tina Daviter, Katherine Smollett, Emilie Mahieu, Susanne Erdmann, Philip Tinnefeld, Roger Garrett, Dina Grohmann, Juri Rappsilber, Finn Werner
Little is known about how archaeal viruses perturb the transcription machinery of their hosts. Here we provide the first example of an archaeo-viral transcription factor that directly targets the host RNA polymerase (RNAP) and efficiently represses its activity. ORF145 from the temperate Acidianus two-tailed virus (ATV) forms a high-affinity complex with RNAP by binding inside the DNA-binding channel where it locks the flexible RNAP clamp in one position. This counteracts the formation of transcription pre-initiation complexes in vitro and represses abortive and productive transcription initiation, as well as elongation...
November 24, 2016: Nature Communications
https://www.readbyqxmd.com/read/27876889/harnessing-hyperthermostable-lactonase-from-sulfolobus-solfataricus-for-biotechnological-applications
#6
Benjamin Rémy, Laure Plener, Laetitia Poirier, Mikael Elias, David Daudé, Eric Chabrière
Extremozymes have gained considerable interest as they could meet industrial requirements. Among these, SsoPox is a hyperthermostable enzyme isolated from the archaeon Sulfolobus solfataricus. This enzyme is a lactonase catalyzing the hydrolysis of acyl-homoserine lactones; these molecules are involved in Gram-negative bacterial communication referred to as quorum sensing. SsoPox exhibits promiscuous phosphotriesterase activity for the degradation of organophosphorous chemicals including insecticides and chemical warfare agents...
November 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27864169/increasing-the-thermostable-sugar-1-phosphate-nucleotidylyltransferase-activities-of-archaeal-st0452-protein-through-site-saturation-mutagenesis-of-the-97th-amino-acid-position
#7
Yuki Honda, Qian Zang, Yasuhiro Shimizu, Mohammad Dadashipour, Zilian Zhang, Yutaka Kawarabayasi
: The ST0452 protein is a bifunctional protein exhibiting sugar-1-phosphate nucleotidylyltransferase (Sugar-1-P NTase) and amino-sugar-1-phosphate acetyltransferase activities and was isolated from the thermophilic archaeon Sulfolobus tokodaii Based on the previous observation that five single mutations increased ST0452 Sugar-1-P NTase activity, nine double mutant ST0452 proteins were generated with the intent of obtaining enzymes exhibiting a further increase in catalysis, but all showed less than 15% of the wild-type N-acetyl-D-glucosamine-1-phosphate uridyltransferase (GlcNAc-1-P UTase) activity...
November 18, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27852927/evolution-of-trnaphe-img2-methyltransferases-involved-in-the-biosynthesis-of-wyosine-derivatives-in-archaea
#8
Jaunius Urbonavičius, Rasa Rutkienė, Anželika Lopato, Daiva Tauraitė, Jonita Stankevičiūtė, Agota Aučynaitė, Laura Kaliniene, Herman van Tilbeurgh, Rolandas Meškys
Tricyclic wyosine derivatives are found at position 37 of eukaryotic and archaeal tRNA(Phe) In Archaea, the intermediate imG-14 is targeted by three different enzymes that catalyze the formation of yW-86, imG, and imG2. We have suggested previously that a peculiar methyltransferase (aTrm5a/Taw22) likely catalyzes two distinct reactions: N(1)-methylation of guanosine to yield m(1)G; and C(7)-methylation of imG-14 to yield imG2. Here we show that the recombinant aTrm5a/Taw22-like enzymes from both Pyrococcus abyssi and Nanoarchaeum equitans indeed possess such dual specificity...
December 2016: RNA
https://www.readbyqxmd.com/read/27822701/aglh-a-thermophilic-udp-n-acetylglucosamine-1-phosphate-dolichyl-phosphate-glcnac-1-phosphotransferase-initiating-protein-n-glycosylation-pathway-in-sulfolobus-acidocaldarius-is-capable-of-complementing-the-eukaryal-alg7
#9
Benjamin H Meyer, Hosam Shams-Eldin, Sonja-Verena Albers
AglH, a predicted UDP-GlcNAc-1-phosphate:dolichyl phosphate GlcNAc-1-phosphotransferase, is initiating the protein N-glycosylation pathway in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius. AglH successfully replaced the endogenous GlcNAc-1-phosphotransferase activity of Alg7 in a conditional lethal Saccharomyces cerevisiae strain, in which the first step of the eukaryal protein N-glycosylation process was repressed. This study is one of the few examples of cross-domain complementation demonstrating a conserved polyprenyl phosphate transferase reaction within the eukaryal and archaeal domain like it was demonstrated for Methanococcus voltae (Shams-Eldin et al...
November 7, 2016: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/27806303/hydroxyurea-mediated-cytotoxicity-without-inhibition-of-ribonucleotide-reductase
#10
Li Phing Liew, Zun Yi Lim, Matan Cohen, Ziqing Kong, Lisette Marjavaara, Andrei Chabes, Stephen D Bell
In many organisms, hydroxyurea (HU) inhibits class I ribonucleotide reductase, leading to lowered cellular pools of deoxyribonucleoside triphosphates. The reduced levels for DNA precursors is believed to cause replication fork stalling. Upon treatment of the hyperthermophilic archaeon Sulfolobus solfataricus with HU, we observe dose-dependent cell cycle arrest, accumulation of DNA double-strand breaks, stalled replication forks, and elevated levels of recombination structures. However, Sulfolobus has a HU-insensitive class II ribonucleotide reductase, and we reveal that HU treatment does not significantly impact cellular DNA precursor pools...
November 1, 2016: Cell Reports
https://www.readbyqxmd.com/read/27799846/characterization-of-a-thermostable-8-oxoguanine-dna-glycosylase-specific-for-go-n-mismatches-from-the-thermoacidophilic-archaeon-thermoplasma-volcanium
#11
Miki Fujii, Chieri Hata, Munetada Ukita, Chie Fukushima, Chihiro Matsuura, Yoshie Kawashima-Ohya, Koji Tomobe, Tsuyoshi Kawashima
The oxidation of guanine (G) to 7,8-dihydro-8-oxoguanine (GO) forms one of the major DNA lesions generated by reactive oxygen species (ROS). The GO can be corrected by GO DNA glycosylases (Ogg), enzymes involved in base excision repair (BER). Unrepaired GO induces mismatched base pairing with adenine (A); as a result, the mismatch causes a point mutation, from G paired with cytosine (C) to thymine (T) paired with adenine (A), during DNA replication. Here, we report the characterization of a putative Ogg from the thermoacidophilic archaeon Thermoplasma volcanium...
2016: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/27794528/an-mcm4-mutation-detected-in-cancer-cells-affects-mcm4-6-7-complex-formation
#12
Ruriko Tatsumi, Yukio Ishimi
An MCM4 mutation detected in human cancer cells from endometrium was characterized. The mutation of G486D is located within MCM-box and the glycine at 486 in human MCM4 is conserved in Saccharomyces cerevisiae MCM4 and Sulfolobus solfataricus MCM. This MCM4 mutation affected human MCM4/6/7 complex formation, since the complex containing the mutant MCM4 protein is unstable and the mutant MCM4 protein is tend to be degraded. It is likely that the MCM4 mutation affects the interaction with MCM7 to destabilize the MCM4/6/7 complex...
October 29, 2016: Journal of Biochemistry
https://www.readbyqxmd.com/read/27781345/immobilization-of-a-bacterial-cytochrome-p450-monooxygenase-system-on-a-solid-support
#13
Cheau Yuaan Tan, Hidehiko Hirakawa, Risa Suzuki, Tomoaki Haga, Fumiya Iwata, Teruyuki Nagamune
Bacterial cytochrome P450s (P450s), which catalyze regio- and stereoselective oxidations of hydrocarbons with high turnover rates, are attractive biocatalysts for fine chemical production. Enzyme immobilization is needed for cost-effective industrial manufacturing. However, immobilization of P450s is difficult because electron-transfer proteins are involved in catalysis and anchoring these can prevent them from functioning as shuttle molecules for carrying electrons. We studied a heterotrimeric protein-mediated co-immobilization of a bacterial P450, and its electron-transfer protein and reductase...
October 26, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27777086/interdomain-interactions-rearrangements-control-the-reaction-steps-of-a-thermostable-dna-alkyltransferase
#14
Castrese Morrone, Riccardo Miggiano, Mario Serpe, Alberto Massarotti, Anna Valenti, Giovanni Del Monaco, Mosè Rossi, Franca Rossi, Menico Rizzi, Giuseppe Perugino, Maria Ciaramella
BACKGROUND: Alkylated DNA-protein alkyltransferases (AGTs) are conserved proteins that repair alkylation damage in DNA by using a single-step mechanism leading to irreversible alkylation of the catalytic cysteine in the active site. Trans-alkylation induces inactivation and destabilization of the protein, both in vitro and in vivo, likely triggering conformational changes. A complete picture of structural rearrangements occurring during the reaction cycle is missing, despite considerable interest raised by the peculiarity of AGT reaction, and the contribution of a functional AGT in limiting the efficacy of chemotherapy with alkylating drugs...
October 22, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27771939/expanding-the-archaellum-regulatory-network-the-eukaryotic-protein-kinases-arnc-and-arnd-influence-motility-of-sulfolobus-acidocaldarius
#15
Lena Hoffmann, Andreas Schummer, Julia Reimann, Maria F Haurat, Amanda J Wilson, Morgan Beeby, Bettina Warscheid, Sonja-V Albers
Expression of the archaellum, the archaeal-type IV pilus-like rotating motility structure is upregulated under nutrient limitation. This is controlled by a network of regulators, called the archaellum regulatory network (arn). Several of the components of this network in Sulfolobus acidocaldarius can be phosphorylated, and the deletion of the phosphatase PP2A results in strongly increased motility during starvation, indicating a role for phosphorylation in the regulation of motility. Analysis of the motility of different protein kinase deletion strains revealed that deletion of saci_0965, saci_1181, and saci_1193 resulted in reduced motility, whereas the deletion of saci_1694 resulted in hypermotility...
October 22, 2016: MicrobiologyOpen
https://www.readbyqxmd.com/read/27768841/o-6-2-deoxyguanosine-butylene-o-6-2-deoxyguanosine-dna-interstrand-cross-links-are-replication-blocking-and-mutagenic-dna-lesions
#16
Wenyan Xu, Daniel Kool, Derek K O'Flaherty, Ashley M Keating, Lauralicia Sacre, Martin Egli, Anne Noronha, Christopher J Wilds, Linlin Zhao
DNA interstrand cross-links (ICLs) are cytotoxic DNA lesions derived from reactions of DNA with a number of anti-cancer reagents as well as endogenous bifunctional electrophiles. Deciphering the DNA repair mechanisms of ICLs is important for understanding the toxicity of DNA cross-linking agents and for developing effective chemotherapies. Previous research has focused on ICLs cross-linked with the N7 and N2 atoms of guanine as well as those formed at the N6 atom of adenine; however, little is known about the mutagenicity of O(6)-dG-derived ICLs...
November 4, 2016: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/27766846/insights-into-the-structure-of-sulfolobus-nucleoid-using-engineered-sac7d-dimers-with-defined-orientation
#17
Gokul Turaga, Stephen P Edmondson, Kelley Smith, John William Shriver
The structure of Archaeal chromatin or nucleoid is believed to have characteristics similar to that found in both eukaryotes and bacteria. Recent comparative studies have suggested that DNA compaction in Archaea requires a bridging protein (e.g. Alba) along with either a wrapping protein (e.g. a histone) or a bending protein such as Sac7d. While x-ray crystal structures demonstrate that Sac7d binds as a monomer to create a significant kink in duplex DNA, the structure of a multiprotein-DNA complex has not been established...
October 21, 2016: Biochemistry
https://www.readbyqxmd.com/read/27752237/discovering-antioxidant-molecules-in-the-archaea-domain-peroxiredoxin-bcp1-from-sulfolobus-solfataricus-protects-h9c2-cardiomyoblasts-from-oxidative-stress
#18
Carmen Sarcinelli, Gabriella Fiorentino, Elio Pizzo, Simonetta Bartolucci, Danila Limauro
Peroxiredoxins (Prxs) are ubiquitous thiol peroxidases that are involved in the reduction of peroxides. It has been reported that prokaryotic Prxs generally show greater structural robustness than their eukaryotic counterparts, making them less prone to inactivation by overoxidation. This difference has inspired the search for new antioxidants from prokaryotic sources that can be used as possible therapeutic biodrugs. Bacterioferritin comigratory proteins (Bcps) of the hyperthermophilic archaeon Sulfolobus solfataricus that belong to the Prx family have recently been characterized...
2016: Archaea: An International Microbiological Journal
https://www.readbyqxmd.com/read/27743417/plasticity-of-archaeal-c-d-box-srna-biogenesis
#19
Vanessa Tripp, Roman Martin, Alvaro Orell, Omer S Alkhnbashi, Rolf Backofen, Lennart Randau
Archaeal and eukaryotic organisms contain sets of C/D box s(no)RNAs with guide sequences that determine ribose 2'-O-methylation sites of target RNAs. The composition of these C/D box sRNA sets is highly variable between organisms and results in varying RNA modification patterns which are important for ribosomal RNA folding and stability. Little is known about the genomic organization of C/D box sRNA genes in archaea. Here, we aimed to obtain first insights into the biogenesis of these archaeal C/D box sRNAs and analyzed the genetic context of more than 300 archaeal sRNA genes...
October 14, 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27738883/backbone-assignment-of-the-binary-complex-of-the-full-length-sulfolobus-solfataricus-dna-polymerase-iv-and-dna
#20
Eunjeong Lee, Jason D Fowler, Zucai Suo, Zhengrong Wu
Sulfolobus solfataricus DNA polymerase IV (Dpo4), a model Y-family DNA polymerase, bypasses a wide range of DNA lesions in vitro and in vivo. In this paper, we report the backbone chemical shift assignments of the full length Dpo4 in its binary complex with a 14/14-mer DNA substrate. Upon DNA binding, several β-stranded regions in the isolated catalytic core and little finger/linker fragments of Dpo4 become more structured. This work serves as a foundation for our ongoing investigation of conformational dynamics of Dpo4 and future determination of the first solution structures of a DNA polymerase and its binary and ternary complexes...
October 13, 2016: Biomolecular NMR Assignments
keyword
keyword
25184
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"