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https://www.readbyqxmd.com/read/29739976/nadh-nadph-bi-cofactor-utilizing-and-thermoactive-ketol-acid-reductoisomerase-from-sulfolobus-acidocaldarius
#1
Chin-Yu Chen, Tzu-Ping Ko, Kuan-Fu Lin, Bo-Lin Lin, Chun-Hsiang Huang, Cheng-Hung Chiang, Jia-Cherng Horng
Ketol-acid reductoisomerase (KARI) is a bifunctional enzyme in the second step of branched-chain amino acids biosynthetic pathway. Most KARIs prefer NADPH as a cofactor. However, KARI with a preference for NADH is desirable in industrial applications including anaerobic fermentation for the production of branched-chain amino acids or biofuels. Here, we characterize a thermoacidophilic archaeal Sac-KARI from Sulfolobus acidocaldarius and present its crystal structure at a 1.75-Å resolution. By comparison with other holo-KARI structures, one sulphate ion is observed in each binding site for the 2'-phosphate of NADPH, implicating its NADPH preference...
May 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29684348/crystal-structure-of-a-thermophilic-o-6-alkylguanine-dna-alkyltransferase-derived-self-labeling-protein-tag-in-covalent-complex-with-a-fluorescent-probe
#2
Franca Rossi, Castrese Morrone, Alberto Massarotti, Davide Maria Ferraris, Anna Valenti, Giuseppe Perugino, Riccardo Miggiano
The self-labeling protein tags are robust and versatile tools for studying different molecular aspects of cell biology. In order to be suitable for a wide spectrum of experimental conditions, it is mandatory that these systems are stable after the fluorescent labeling reaction and do not alter the properties of the fusion partner. SsOGT-H5 is an engineered variant alkylguanine-DNA-alkyl-transferase (OGT) of the hyperthermophilic archaeon Sulfolobus solfataricus, and it represents an alternative solution to the SNAP-tag® technology under harsh reaction conditions...
April 20, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29679495/insights-into-the-post-translational-modifications-of-archaeal-sis10b-alba-lysine-16-is-methylated-not-acetylated-and-this-does-not-regulate-transcription-or-growth
#3
Jingjing Cao, Qian Wang, Tao Liu, Nan Peng, Li Huang
Nucleic acid-binding proteins of the Sac10b family, also referred to as Alba (for acetylation lowers binding affinity), are highly conserved in Archaea. It was reported that Sso10b, a Sac10b homologue from Sulfolobus solfataricus, was acetylated at the ε-amino group of K16 and the α-amino group of the N-terminal residue. Notably, acetylation of K16 reduced the affinity of Sso10b for DNA and de-repressed transcription in vitro. Here we show that Sis10b, a Sac10b homologue from Sulfolobus islandicus, underwent a range of post-translational modifications (PTMs)...
April 21, 2018: Molecular Microbiology
https://www.readbyqxmd.com/read/29679096/protein-evolution-is-potentially-governed-by-protein-stability-directed-evolution-of-an-esterase-from-the-hyperthermophilic-archaeon-sulfolobus-tokodaii
#4
Ryo Kurahashi, Satoshi Sano, Kazufumi Takano
The study of evolution is important to understand biological phenomena. During evolutionary processes, genetic changes confer amino acid substitutions in proteins, resulting in new or improved functions. Unfortunately, most mutations destabilize proteins. Thus, protein stability is a significant factor in evolution; however, its role remains unclear. Here, we simply and directly explored the association between protein activity and stability in random mutant libraries to elucidate the role of protein stability in evolutionary processes...
April 20, 2018: Journal of Molecular Evolution
https://www.readbyqxmd.com/read/29671720/sulfurisphaera-javensis-sp-nov-a-hyperthermophilic-and-acidophilic-archaeon-isolated-from-indonesian-hot-spring-and-reclassification-of-sulfolobus-tokodaii-suzuki-et-al-2002-as-sulfurisphaera-tokodaii-comb-nov
#5
Kazuhiko Tsuboi, Hiroyuki D Sakai, Naswandi Nur, Kenneth M Stedman, Norio Kurosawa, Antonius Suwanto
A novel hyperthermophilic, acidophilic and facultatively anaerobic archaeon, strain KD-1T , was isolated from an acidic hot spring in Indonesia and characterized with the phylogenetically related species Sulfurisphaera ohwakuensis Kurosawa et al. 1998, Sulfolobus tokodaii Suzuki et al., 2002 and Sulfolobus yangmingensis Jan et al. 1999. Cells of KD-1T were irregular cocci with diameters of 0.9-1.3 µm. The strain grew at 60-90 °C (optimum 80-85 °C), pH 2.5-6.0 (optimum pH 3.5-4.0) and 0-1.0 % (w/v) NaCl concentration...
April 19, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29663675/a-stable-artificial-multienzymatic-complex-using-a-heterotrimeric-protein-from-metallosphaera-sedula
#6
Fumiya Iwata, Hidehiko Hirakawa, Teruyuki Nagamune
Bacterial cytochrome P450 monooxygenases (P450s) are promising biocatalysts for chemical syntheses because they catalyze a variety of oxidations on non-activated hydrocarbons using O2 . However, the requirement of two auxiliary proteins, an electron transfer protein and a reductase, for the catalysis is a major bottleneck for in vitro applications of these monooxygenases. Our previous study showed that artificial assembly of a bacterial P450 with its auxiliary proteins using a heterotrimeric proliferating cell nuclear antigen (PCNA) from Sulfolobus solfataricus yields a self-sufficient P450, but partial dissociation of P450 from the complex at catalytic concentrations reduces the apparent specific activity of this self-sufficient P450...
April 16, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29629622/tolerance-of-sulfolobus-smv1-virus-to-the-immunity-of-i-a-and-iii-b-crispr-cas-systems-in-sulfolobus-islandicus
#7
Tong Guo, Wenyuan Han, Qunxin She
Sulfolobus islandicus Rey15A encodes one type I-A and two type III-B systems, all of which are active in mediating nucleic acids interference. However, the effectiveness of each CRISPR system against virus infection was not tested in this archaeon. Here we constructed S. islandicus strains that constitutively express the antiviral immunity from either I-A, or III-B, or I-A plus III-B systems against SMV1 and tested the response of each host to SMV1 infection. We found that, although both CRISPR immunities showed a strongly inhibition to viral DNA replication at an early stage of incubation, the host I-A CRISPR immunity gradually lost the control on virus proliferation, allowing accumulation of cellular viral DNA and release of a large number of viral particles...
April 9, 2018: RNA Biology
https://www.readbyqxmd.com/read/29625930/cloning-expression-and-characterization-of-the-maltooligosyl-trehalose-synthase-from-the-archaeon-sulfolobus-tokodaii
#8
Meng Zhao, Xiangqun Xu, Shaolong Yang, Tianming Liu, Bo Liu
The maltooligosyl trehalose synthase gene from the hyperthermophilic archaeon Sulfolobus tokodaii strain 7 was cloned and the recombinant peotein was expressed in E. coli. The protein was purified to homogeneity by nickel column chromatography. The archaeal enzyme could catalyze an intramolecular transglycosylation reaction and convert the glycosidic bond at the reducing end of dextrins from α-1, 4 (reducing end) into α-1, 1 (non-reducing end). The most suitable temperature was 75°C and the optimal pH was 5...
March 2018: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29618058/a-transcriptional-factor-b-paralog-functions-as-an-activator-to-dna-damage-responsive-expression-in-archaea
#9
Xu Feng, Mengmeng Sun, Wenyuan Han, Yun Xiang Liang, Qunxin She
Previously it was shown that UV irradiation induces a strong upregulation of tfb3 coding for a paralog of the archaeal transcriptional factor B (TFB) in Sulfolobus solfataricus, a crenarchaea. To investigate the function of this gene in DNA damage response (DDR), tfb3 was inactivated by gene deletion in Sulfolobus islandicus and the resulting Δtfb3 was more sensitive to DNA damage agents than the original strain. Transcriptome analysis revealed that a large set of genes show TFB3-dependent activation, including genes of the ups operon and ced system...
March 30, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29608017/improvement-of-functional-properties-of-a-thermostable-%C3%AE-glycosidase-for-milk-lactose-hydrolysis
#10
Elena Ionata, Loredana Marcolongo, Francesco La Cara, Giovanni P Cetrangolo, Ferdinando Febbraio
In this work, a new exploitation of the thermostable β-glycosidase from Sulfolobus solfataricus expressed in Saccharomyces cerevisiae to create functional foods for low lactose diets was evaluated. For this purpose, the lactose hydrolysis reaction using immobilized and soluble enzymes was investigated. Activity and stability at different conditions of pH and temperature were tested. The immobilization process had a big impact on the catalysis performance, leading to an enhancement of the enzymatic reaction rate on lactose, as demonstrated by the increasing of 2 and 2...
April 2, 2018: Biopolymers
https://www.readbyqxmd.com/read/29605923/expression-purification-and-assembly-of-archaellum-subcomplexes-of-sulfolobus-acidocaldarius
#11
Paushali Chaudhury, Patrick Tripp, Sonja-Verena Albers
The archaellum assembly machinery and its filament consist of seven proteins in the crenarchaeon Sulfolobus acidocaldarius. We have so far expressed, purified, and biochemically characterized four of these archaellum subunits, namely, FlaX, FlaH, FlaI, and FlaF. FlaX, FlaH, and FlaI tightly interact and form the archaellum motor complex important for archaellum assembly and rotation. We have previously shown that FlaH forms an inner ring within a very stable FlaX ring, and therefore FlaX is believed to provide the scaffold for the assembly of the archaellum motor complex...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29574614/two-trehalose-hydrolyzing-enzymes-from-crenarchaeon-sulfolobus-acidocaldarius-exhibit-distinct-activities-and-affinities-toward-trehalose
#12
Mitsuhiro Yuasa, Takeshi Okamura, Masahiro Kimura, Shotaro Honda, Yongchol Shin, Masao Kawakita, Fumitaka Oyama, Masayoshi Sakaguchi
Two archaeal trehalase-like genes, Saci1250 and Saci1816, belonging to glycoside hydrolase family 15 (GH15) from the acidophilic Crenarchaeon Sulfolobus acidocaldarius were expressed in Escherichia coli. The gene products showed trehalose-hydrolyzing activities, and the names SaTreH1 and SaTreH2 were assigned to Saci1816 and Saci1250 gene products, respectively. These newly identified enzymes functioned within a narrow range of acidic pH values at elevated temperatures, which is similar to the behavior of Euryarchaeota Thermoplasma trehalases...
March 24, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29558934/high-yield-production-and-purification-of-two-recombinant-thermostable-phosphotriesterase-like-lactonases-from-sulfolobus-acidocaldarius-and-sulfolobus-solfataricus-useful-as-bioremediation-tools-and-bioscavengers
#13
Odile Francesca Restaino, Maria Giovanna Borzacchiello, Ilaria Scognamiglio, Luigi Fedele, Alberto Alfano, Elena Porzio, Giuseppe Manco, Mario De Rosa, Chiara Schiraldi
BACKGROUND: Thermostable phosphotriesterase-like lactonases (PLLs) are able to degrade organophosphates and could be potentially employed as bioremediation tools and bioscavengers. But nowadays their manufacturing in high yields is still an issue that limits their industrial applications. In this work we aimed to set up a high yield production and purification biotechnological process of two recombinant PLLs expressed in E. coli, the wild type SacPox from Sulfolobus acidocaldarius and a triple mutated SsoPox C258L/I261F/W263A, originally from Sulfolobus solfataricus...
March 20, 2018: BMC Biotechnology
https://www.readbyqxmd.com/read/29541080/novel-bioengineered-cassava-expressing-an-archaeal-starch-degradation-system-and-a-bacterial-adp-glucose-pyrophosphorylase-for-starch-self-digestibility-and-yield-increase
#14
Ayalew Ligaba-Osena, Jenna Jones, Emmanuel Donkor, Sanjeev Chandrayan, Farris Pole, Chang-Hao Wu, Claire Vieille, Michael W W Adams, Bertrand B Hankoua
To address national and global low-carbon fuel targets, there is great interest in alternative plant species such as cassava ( Manihot esculenta ), which are high-yielding, resilient, and are easily converted to fuels using the existing technology. In this study the genes encoding hyperthermophilic archaeal starch-hydrolyzing enzymes, α-amylase and amylopullulanase from Pyrococcus furiosus and glucoamylase from Sulfolobus solfataricus , together with the gene encoding a modified ADP-glucose pyrophosphorylase ( glgC ) from Escherichia coli , were simultaneously expressed in cassava roots to enhance starch accumulation and its subsequent hydrolysis to sugar...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29540560/erratum-for-mart%C3%A3-nez-alvarez-et-al-formation-of-a-viral-replication-focus-in-sulfolobus-cells-infected-by-the-rudivirus-sulfolobus-islandicus-rod-shaped-virus-2
#15
Laura Martínez-Alvarez, Ling Deng, Xu Peng
No abstract text is available yet for this article.
April 1, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29529283/investigation-of-sliding-dna-clamp-dynamics-by-single-molecule-fluorescence-mass-spectrometry-and-structure-based-modeling
#16
Varun V Gadkari, Sophie R Harvey, Austin T Raper, Wen-Ting Chu, Jin Wang, Vicki H Wysocki, Zucai Suo
Proliferating cell nuclear antigen (PCNA) is a trimeric ring-shaped clamp protein that encircles DNA and interacts with many proteins involved in DNA replication and repair. Despite extensive structural work to characterize the monomeric, dimeric, and trimeric forms of PCNA alone and in complex with interacting proteins, no structure of PCNA in a ring-open conformation has been published. Here, we use a multidisciplinary approach, including single-molecule Förster resonance energy transfer (smFRET), native ion mobility-mass spectrometry (IM-MS), and structure-based computational modeling, to explore the conformational dynamics of a model PCNA from Sulfolobus solfataricus (Sso), an archaeon...
April 6, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29529252/a-regulatory-rna-is-involved-in-rna-duplex-formation-and-biofilm-regulation-in-sulfolobus-acidocaldarius
#17
Alvaro Orell, Vanessa Tripp, Victor Aliaga-Tobar, Sonja-Verena Albers, Vinicius Maracaja-Coutinho, Lennart Randau
Non-coding RNAs (ncRNA) are involved in essential biological processes in all three domains of life. The regulatory potential of ncRNAs in Archaea is, however, not fully explored. In this study, RNA-seq analyses identified a set of 29 ncRNA transcripts in the hyperthermophilic archaeon Sulfolobus acidocaldarius that were differentially expressed in response to biofilm formation. The most abundant ncRNA of this set was found to be resistant to RNase R treatment (RNase R resistant RNA, RrrR(+)) due to duplex formation with a reverse complementary RNA (RrrR(-))...
February 26, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29526782/solution-structure-of-an-archaeal-duf61-family-protein-sso0941-encoded-by-a-gene-in-the-operon-of-box-c-d-rna-protein-complexes
#18
Tao Zhou, Xingzhe Yao, Jinfeng Wang, Yingang Feng
Domain of unknown function 61 (DUF61) family proteins widely exist in archaea and the genes of DUF61 proteins in crenarchaea are in an operon containing two genes of box C/D RNA protein complexes. Here we report the solution NMR structure of DUF61 family member protein SSO0941, from the hyperthermophilic archaeon Sulfolobus solfataricus. SSO0941 has a rigid core structure and flexible N- and C-terminal regions as well as a negatively-charged independent C-terminal helix. The core structure consists of N- and C-terminal subdomains, in which the C-terminal subdomain shows significant structural similarity with several nucleic acid binding proteins...
March 8, 2018: Journal of Structural Biology
https://www.readbyqxmd.com/read/29507349/anti-crispr-proteins-encoded-by-archaeal-lytic-viruses-inhibit-subtype-i-d-immunity
#19
Fei He, Yuvaraj Bhoobalan-Chitty, Lan B Van, Anders L Kjeldsen, Matteo Dedola, Kira S Makarova, Eugene V Koonin, Ditlev E Brodersen, Xu Peng
Viruses employ a range of strategies to counteract the prokaryotic adaptive immune system, clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas), including mutational escape and physical blocking of enzymatic function using anti-CRISPR proteins (Acrs). Acrs have been found in many bacteriophages but so far not in archaeal viruses, despite the near ubiquity of CRISPR-Cas systems in archaea. Here, we report the functional and structural characterization of two archaeal Acrs from the lytic rudiviruses, SIRV2 and SIRV3...
April 2018: Nature Microbiology
https://www.readbyqxmd.com/read/29507091/identification-of-a-direct-biosynthetic-pathway-for-udp-n-acetylgalactosamine-from-glucosamine-6-phosphate-in-thermophilic-crenarchaeon-sulfolobus-tokodaii
#20
Mohammad Dadashipour, Mariko Iwamoto, Mohammad Murad Hossain, Jun-Ichi Akutsu, Zilian Zhang, Yutaka Kawarabayasi
Most organisms, from Bacteria to Eukarya , synthesize UDP- N -acetylglucosamine (UDP-GlcNAc) from fructose-6-phosphate via a four-step reaction, and UDP- N -acetylgalactosamine (UDP-GalNAc) can only be synthesized from UDP-GlcNAc by UDP-GlcNAc 4-epimerase. In Archaea , the bacterial-type UDP-GlcNAc biosynthetic pathway was reported for Methanococcales. However, the complete biosynthetic pathways for UDP-GlcNAc and UDP-GalNAc present in one archaeal species are unidentified. Previous experimental analyses on enzymatic activities of the ST0452 protein, identified from the thermophilic crenarchaeon Sulfolobus tokodaii , predicted the presence of both a bacterial-type UDP-GlcNAc and an independent UDP-GalNAc biosynthetic pathway in this archaeon...
May 15, 2018: Journal of Bacteriology
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