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Keywords macrolide resistances staphylo...

macrolide resistances staphylococcus aureus PCR genes

https://read.qxmd.com/read/35760653/phenotypic-and-molecular-characterization-of-staphylococcus-aureus-isolates-conducted-in-nares-of-psoriatic-patients-attending-a-public-hospital-in-argentina
#21
JOURNAL ARTICLE
Carina A Boncompain, Cristian A Suárez, Mario Squeff, Valeria Belluzo, Gustavo Piccirilli, Ana Molteni, Ramón A Fernández Bussy, Héctor R Morbidoni
Although Staphylococcus aureus increases its relative abundance in psoriasis when compared with the microbiome of healthy subjects, it is not the most important microorganism underlying this disease. However, there is scant data on the role and molecular features of S. aureus strains in psoriasis; therefore, the aim of this study was to evaluate nasal carriage of this microorganism, its phenotypic and molecular characteristics as well as the impact of host factors on its carriage in psoriatic patients. The presence of S...
June 24, 2022: Revista Argentina de Microbiología
https://read.qxmd.com/read/35711812/a-small-multihost-plasmid-carrying-erm-t-identified-in-enterococcus-faecalis
#22
JOURNAL ARTICLE
Xing-Yun Li, Rui Yu, Chunyan Xu, Yanhong Shang, Dexi Li, Xiang-Dang Du
The aim of this study was to determine the mobile genetic elements involved in the horizontal transfer of erm (T) in Enterococcus faecalis , and its transmission ability in heterologous hosts. A total of 159 erythromycin-resistant enterococci isolates were screened for the presence of macrolide resistance genes by PCR. Whole genome sequencing for erm (T)-carrying E. faecalis E165 was performed. The transmission ability in heterologous hosts was explored by conjugation, transformation, and fitness cost. The erm (T) gene was detected only in an E...
2022: Frontiers in Veterinary Science
https://read.qxmd.com/read/35656950/high-prevalence-of-multi-drug-resistant-methicillin-resistant-staphylococcus-aureus-in-tertiary-egyptian-hospitals
#23
JOURNAL ARTICLE
Abdel-Aty E Alfeky, Mahmoud M Tawfick, Mohamed S Ashour, Abdel-Nasser A El-Moghazy
INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is an emerging cause of morbidity and mortality worldwide. This work aimed to study the occurrence of multidrug-resistant MRSA (MDR-MRSA) in tertiary Egyptian hospitals and determine the antimicrobial susceptibilities and the genetic relatedness of isolates for epidemiological assessment. METHODOLOGY: A total of 170 S. aureus isolates were collected from two Egyptian tertiary hospitals in Cairo, between September 2017 and December 2018...
May 30, 2022: Journal of Infection in Developing Countries
https://read.qxmd.com/read/34940405/inducible-clindamycin-resistance-and-biofilm-production-among-staphylococci-isolated-from-tertiary-care-hospitals-in-nepal
#24
JOURNAL ARTICLE
Sarita Manandhar, Raju Shrestha, Ratna Shova Tuladhar, Sunil Lekhak
Resistance to antibiotics, biofilm formation and the presence of virulence factors play important roles in increased mortality associated with infection by staphylococci. The macrolide lincosamide streptogramin B (MLSB ) family of antibiotics is commonly used to treat infections by methicillin-resistant isolates. Clinical failure of clindamycin therapy has been reported due to multiple mechanisms that confer resistance to MLSB . This study aims to find the incidence of different phenotypes of MLSB resistance and biofilm production among staphylococci...
December 7, 2021: Infectious Disease Reports
https://read.qxmd.com/read/34665771/identification-and-characterization-of-vancomycin-resistant-staphylococcus-aureus-in-hospital-wastewaters-evidence-of-horizontal-spread-of-antimicrobial-resistance
#25
JOURNAL ARTICLE
Sneha Kalasseril Girijan, Devika Pillai
Antibiotic resistance has become a major threat to human health around the world, but its spread through the aquatic environment has been often overlooked. This study aimed to determine the occurrence of vancomycin-resistant Staphylococcus aureus in hospital wastewaters and their transmission into public water bodies in Kerala, India. A total of 113 S. aureus were isolated from three hospital effluents in Kerala, India. Standard disc diffusion and the strip method were used for antibiotic susceptibility testing and minimum inhibitory concentration detection...
October 2021: Journal of Water and Health
https://read.qxmd.com/read/34530296/swine-as-reservoirs-of-zoonotic-borderline-oxacillin-resistant-staphylococcus-aureus-st398
#26
JOURNAL ARTICLE
S C L Santos, M M S Saraiva, A L B Moreira Filho, N M V Silva, C M G De Leon, L A F Pascoal, P E N Givisiez, W A Gebreyes, C J B Oliveira
Methicillin resistance mediated by the mecA gene in Staphylococcus aureus, also known as "true MRSA", is typically associated with high oxacillin MIC values (≥8 mg/L). Because non-mecA-mediated oxacillin resistant S. aureus phenotypes can also cause hard-to-treat diseases in humans, their misidentification as methicillin-susceptible S. aureus strains (MSSA) can compromise the efficiency of the antimicrobial therapy. These strains have been refereed as Borderline Oxacillin-Resistant S. aureus (BORSA) but their characterization and role in clinical microbiology have been neglected...
December 2021: Comparative Immunology, Microbiology and Infectious Diseases
https://read.qxmd.com/read/34356479/association-of-macrolide-resistance-genotypes-and-synergistic-antibiotic-combinations-for-combating-macrolide-resistant-mrsa-recovered-from-hospitalized-patients
#27
JOURNAL ARTICLE
Amr S Bishr, Salma M Abdelaziz, Ibrahim S Yahia, Mahmoud A Yassien, Nadia A Hassouna, Khaled M Aboshanab
Macrolide-resistant methicillin-resistant Staphylococcus aureus (MAC-MRSA) is one of the most clinically relevant pathogens due to its significant ability of resistance acquisition to different antimicrobial agents. This study aimed to evaluate antimicrobial susceptibility and the use of different combinations of azithromycin with other antibiotics for combating MAC resistance. Seventy-two Staphylococci (38.5%) (n = 187), showed resistance to MACs; of these, 53 isolates (73.6%, n = 72) were S. aureus and 19 (26...
July 6, 2021: Biology
https://read.qxmd.com/read/34071800/molecular-characterization-of-antimicrobial-resistance-and-virulence-genes-of-bacterial-pathogens-from-bovine-and-caprine-mastitis-in-northern-lebanon
#28
JOURNAL ARTICLE
Zahie Abboud, Lucia Galuppo, Marco Tolone, Maria Vitale, Roberto Puleio, Marwan Osman, Guido Ruggero Loria, Monzer Hamze
Mastitis is an infectious disease encountered in dairy animals worldwide that is currently a growing concern in Lebanon. This study aimed at investigating the etiology of the main mastitis-causing pathogens in Northern Lebanon, determining their antimicrobial susceptibility profiles, and identifying their antimicrobial resistance (AMR) genes. A total of 101 quarter milk samples were collected from 77 cows and 11 goats presenting symptoms of mastitis on 45 dairy farms. Bacterial identification was carried out through matrix-assisted laser desorption/ionization-time of flight mass spectrometry...
May 27, 2021: Microorganisms
https://read.qxmd.com/read/33925700/virulence-and-drug-resistance-of-staphylococcus-aureus-strains-isolated-from-venous-ulcers-in-polish-patients
#29
JOURNAL ARTICLE
Mateusz Gajda, Emilia Załugowicz, Monika Pomorska-Wesołowska, Tomasz Bochenek, Barbara Gryglewska, Dorota Romaniszyn, Agnieszka Chmielarczyk, Jadwiga Wójkowska-Mach
Infected chronic venous ulcers (VUs) represent a major health problem. We analysed the aerobic microbiome in the VUs, the virulence, and drug-resistance of Staphylococcus aureus (SA) strains. Swabs from 143 outpatients and inpatients Polish subjects were collected. SA strains were tested for drug sensitivity using a phenotyping method and for methicillin-resistant SA (MRSA) and macrolide-lincosamide-streptogramin B (MLSB) resistance using PCR. We analysed virulence genes, the genetic similarity of strains, and performed Staphylococcal cassette chromosome mec typing and Staphylococcal protein A typing...
April 27, 2021: International Journal of Environmental Research and Public Health
https://read.qxmd.com/read/33902574/antimicrobial-resistance-and-genotyping-of-staphylococcus-aureus-obtained-from-food-animals-in-sichuan-province-china
#30
JOURNAL ARTICLE
Ting Gan, Gang Shu, Hualin Fu, Qigui Yan, Wei Zhang, Huaqiao Tang, Lizi Yin, Ling Zhao, Juchun Lin
BACKGROUND: Staphylococcus aureus (S. aureus), especially methicillin-resistant Staphylococcus aureus (MRSA), is considered a common zoonotic pathogen, causing severe infections. The objective of this study was to investigate the antimicrobial susceptibility, resistance genes and molecular epidemiology among MRSA and methicillin-susceptible Staphylococcus aureus (MSSA) isolated from food animals in Sichuan Province, China. METHODS: This study was conducted on 236 S...
April 26, 2021: BMC Veterinary Research
https://read.qxmd.com/read/33838068/genotypic-and-phenotypic-profiles-of-antibiotic-resistant-bacteria-isolated-from-hospitalised-patients-in-bangladesh
#31
JOURNAL ARTICLE
Kazi Sarjana Safain, Golam Sarower Bhuyan, Saad Hassan Hasib, Mohammad Sazzadul Islam, Mohammad Al Mahmud-Un-Nabi, Rosy Sultana, Sadia Tasnim, Farjana Akther Noor, Suprovath Kumar Sarker, Md Tarikul Islam, Asifuzzaman Rahat, Daniel T Leung, Daryl Domman, Farhana Manzoor, Sajid Anwar, Md Abdul Majid Bhuiyan, Emran Kabir Chowdhury, Syed Saleheen Qadri, Firdausi Qadri, Kaiissar Mannoor
OBJECTIVES: Characterisation of resistance phenotype and genotype is crucial to understanding the burden and transmission of antimicrobial resistance (AMR). This study aims to determine the spectrum of AMR and associated genes encoding aminoglycoside, macrolide and β-lactam classes of antimicrobials in bacteria isolated from hospitalised patients in Bangladesh. METHODS: 430 bacterial isolates from patients with respiratory, intestinal, wound infections and typhoid fever, presenting to clinical care from 2015 to 2019, were examined...
July 2021: Tropical Medicine & International Health
https://read.qxmd.com/read/33296341/inducible-clindamycin-resistance-in-clinical-isolates-of-staphylococcus-aureus-in-suez-canal-university-hospital-ismailia-egypt
#32
JOURNAL ARTICLE
Rania Mohammed Kishk, Maha Mohammed Anani, Nader Attia Nemr, Nashaat Mohamed Soliman, Marwa Mohamed Fouad
INTRODUCTION: The increasing incidence of methicillin resistance among Staphylococci has led to renewed interest in the usage of macrolide-lincosamide-streptogramin B (MLSB) antibiotics to treat S. aureus infections, with clindamycin being the preferable agent owing to its excellent pharmacokinetic properties. Inducible clindamycin resistance my lead to therapeutic failure. AIM: Detection of the prevalence of constitutive and inducible clindamycin resistance in clinical isolates of S...
November 30, 2020: Journal of Infection in Developing Countries
https://read.qxmd.com/read/32646458/the-effect-of-short-course-antibiotics-on-the-resistance-profile-of-colonizing-gut-bacteria-in-the-icu-a-prospective-cohort-study
#33
JOURNAL ARTICLE
Christian Munck, Ravi U Sheth, Edward Cuaresma, Jessica Weidler, Stephania L Stump, Philip Zachariah, David H Chong, Anne-Catrin Uhlemann, Julian A Abrams, Harris H Wang, Daniel E Freedberg
BACKGROUND: The need for early antibiotics in the intensive care unit (ICU) is often balanced against the goal of antibiotic stewardship. Long-course antibiotics increase the burden of antimicrobial resistance within colonizing gut bacteria, but the dynamics of this process are not fully understood. We sought to determine how short-course antibiotics affect the antimicrobial resistance phenotype and genotype of colonizing gut bacteria in the ICU by performing a prospective cohort study with assessments of resistance at ICU admission and exactly 72 h later...
July 9, 2020: Critical Care: the Official Journal of the Critical Care Forum
https://read.qxmd.com/read/32635147/genetic-characterization-of-methicillin-resistant-staphylococcus-aureus-isolates-from-human-bloodstream-infections-detection-of-mls-b-resistance
#34
JOURNAL ARTICLE
Vanessa Silva, Sara Hermenegildo, Catarina Ferreira, Célia M Manaia, Rosa Capita, Carlos Alonso-Calleja, Isabel Carvalho, José Eduardo Pereira, Luis Maltez, José L Capelo, Gilberto Igrejas, Patrícia Poeta
In this study we aimed to characterize antimicrobial resistance in methicillin-resistant Staphylococcus aureus (MRSA) isolated from bloodstream infections as well as the associated genetic lineages of the isolates. Sixteen MRSA isolates were recovered from bacteremia samples from inpatients between 2016 and 2019. The antimicrobial susceptibility of these isolates was tested by the Kirby-Bauer disk diffusion method against 14 antimicrobial agents. To determine the macrolide-lincosamide-streptogramin B (MLSB ) resistance phenotype of the isolates, erythromycin-resistant isolates were assessed by double-disk diffusion (D-test)...
July 3, 2020: Antibiotics
https://read.qxmd.com/read/32607349/antimicrobial-profile-of-multidrug-resistant-streptococcus-spp-isolated-from-dairy-cows-with-clinical-mastitis
#35
JOURNAL ARTICLE
Hala Abd El-Menem Rizk Saed, Hussam Mohamed Mohamed Ibrahim
OBJECTIVE: The current investigation was designed to point out the prevalence of multidrug-resistant Streptococcus spp. causing acute clinical mastitis and their pattern of antibiotic resistance in dairy cows. MATERIALS AND METHODS: Milk was sampled from 128 dairy cows with 191 infected quarters during the period from August 2017 to December 2018. Bacterial species were isolated from the milk samples and identified based on colony morphology and biochemical tests...
June 2020: Journal of Advanced Veterinary and Animal Research
https://read.qxmd.com/read/32566056/prevalence-and-molecular-genetics-of-methicillin-resistant-staphylococcus-aureus-colonization-in-nursing-homes-in-saudi-arabia
#36
JOURNAL ARTICLE
Ahmed Albarrag, Ashwag Shami, Abrar Almutairi, Sara Alsudairi, Sumayh Aldakeel, Amani Al-Amodi
Objective: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main causative agents of nosocomial infections that has posed a major threat to those with compromised immune systems such as nursing home residents. The aim of this study was to determine the rates of MRSA strains and the types of Staphylococcal Cassette Chromosome mec (SCCmec )in nursing homes in Saudi Arabia. Methods: A total of 188 nasal swabs were collected from the residents and nursing staff in two nursing homes in Riyadh, Saudi Arabia...
2020: Canadian Journal of Infectious Diseases & Medical Microbiology
https://read.qxmd.com/read/32368106/molecular-characterization-drug-resistance-and-virulence-analysis-of-constitutive-and-inducible-clindamycin-resistance-staphylococcus-aureus-strains-recovered-from-clinical-samples-tehran-iran
#37
JOURNAL ARTICLE
Mehdi Goudarzi, Zahra Tayebi, Maryam Fazeli, Mirmohammad Miri, Mohammad Javad Nasiri
Background: Macrolide-lincosamide streptogramin B family is one of the important alternative antibiotics for treating staphylococcal infections. The aim of this study was to determine the characteristics and prevalence of antibiotic resistance genes in different coagulase types of clinical Staphylococcus aureus strains. Methods: In the present study, 86 isolates with different phenotypes of MLSB resistance were investigated. In vitro susceptibility was assessed by the disk diffusion and broth microdilution methods...
2020: Infection and Drug Resistance
https://read.qxmd.com/read/32290833/value-of-multiplex-pcr-for-detection-of-antimicrobial-resistance-in-samples-retrieved-from-patients-with-orthopaedic-infections
#38
JOURNAL ARTICLE
Irene Katharina Sigmund, Nora Renz, Susanne Feihl, Christian Morgenstern, Sabrina Cabric, Andrej Trampuz
BACKGROUND: The performance of multiplex PCR (mPCR) for detection of antimicrobial resistance from clinical isolates is unknown. We assessed the ability of mPCR to analyse resistance genes directly from clinical samples. Patients with orthopedic infections were prospectively included. Phenotypical and genotypical resistance was evaluated in clinical samples (synovial and sonication fluid) where identical pathogens were identified by culture and mPCR. RESULT: A total of 94 samples were analysed, including 60 sonication fluid and 34 synovial fluid samples...
April 14, 2020: BMC Microbiology
https://read.qxmd.com/read/32176841/thymidine-auxotrophic-staphylococcus-aureus-small-colony-variant-bacteremia-in-a-patient-with-cystic-fibrosis
#39
JOURNAL ARTICLE
Dilair C de Souza, Laura L Cogo, Jussara K Palmeiro, Libera M Dalla-Costa, Ana P de Oliveira Tomaz, Carlos A Riedi, Nelson A Rosario Filho
BACKGROUND: Small-colony variants (SCVs) are a morphologic subtype of Staphylococcus aureus that may occur through several mechanisms including auxotrophism for thymidine, hemin, or menadione. Auxotrophic SCV for thymidine fail to synthesize DNA specifically because of mutations in the thymidylate synthase gene. We isolated S. aureus thymidine-dependent SCVs (TD-SCV) from blood and respiratory samples of a pediatric patient with cystic fibrosis and pulmonary exacerbation. METHODS: Nutritional dependence of SCVs on hemin, menadione, and thymidine was evaluated...
March 16, 2020: Pediatric Pulmonology
https://read.qxmd.com/read/31971461/in-feed-tylosin-phosphate-administration-to-feedlot-cattle-minimally-affects-antimicrobial-resistance
#40
JOURNAL ARTICLE
John W Schmidt, Amit Vikram, Eric Miller, Shuna A Jones, Terrance M Arthur
The macrolide class antimicrobial tylosin (trade name Tylan) is approved by the U.S. Food and Drug Administration for continuous inclusion in feed for liver abscess prevention. To address concerns that this antimicrobial application may threaten human health, a population of feedlot steers was split into a control treatment ( n = 42) and a tylosin treatment ( n = 42). Feed rations were identical except for the inclusion of tylosin at 60 to 90 mg per head per day. Fecal swab ( n = 335), pen surface material ( n = 256), feed ( n = 56), and water trough ( n = 32) samples were obtained over four sample occasions: November (1 day before the start of tylosin inclusion in feed), January (80 days of tylosin in feed), April (167 days), and June (253 days)...
January 23, 2020: Journal of Food Protection
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