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macrolide resistances staphylococcus aureus PCR genes

Suhaili Zarizal, Chew Chieng Yeo, Ghazali Mohd Faizal, Ching Hoong Chew, Zainul Amiruddin Zakaria, Mazen M Jamil Al-Obaidi, Nordin Syafinaz Amin, Mohd Desa Mohd Nasir
BACKGROUND: This study aimed to profile the antimicrobial susceptibility and presence of resistance and virulence genes of methicillin-susceptible S. aureus (MSSA) and MRSA nasal carriage, by means of genotypic analyses, in students of a tertiary institution in the state of Terengganu, east coast Malaysia. METHODS: 370 agricultural biotechnology students from Universiti Sultan Zainal Abidin in Besut, Terengganu were enrolled in this study. Antimicrobial susceptibility profiles were evaluated by standard methods...
June 6, 2018: Tropical Medicine & International Health: TM & IH
Zarizal Suhaili, Putri 'Amira Rafee, Norhidayah Mat Azis, Chew Chieng Yeo, Syafinaz Amin Nordin, Abdul Rachman Abdul Rahim, Mazen M Jamil Al-Obaidi, Mohd Nasir Mohd Desa
Introduction: This study aims to assess the antimicrobial susceptibility profiles of Staphylococcus aureus strains isolated from university students and to determine the prevalence of constitutive and inducible clindamycin resistance, the latter being able to cause therapeutic failure due to false in vitro clindamycin susceptibility. Methods: S. aureus strains were isolated from the nasal swabs of 200 health sciences students of a Malaysian university. Twelve classes of antibiotics were used to evaluate the antimicrobial susceptibility profiles with the macrolide-lincosamide-streptogramin B (MLSB ) phenotype for inducible clindamycin resistance determined by the double-diffusion test (D-test)...
March 2018: Germs
Hossein Sedaghat, Bahram Nasr Esfahani, Sina Mobasherizadeh, Azhar Sallari Jazi, Mehrdad Halaji, Parisa Sadeghi, Mohammad Emaneini, Seyed Asghar Havaei
Background and Objectives: Macrolide, lincosamide and streptogramin B (MLSB ) are noteworthy antibiotics for the treatment of Staphylococcus aureus infections. The purpose of this study, was to determine the phenotypic and genotypic characterization of macrolide resistance, among S. aureus , isolated from clinical samples and nasal swabs. Materials and Methods: Totally, 162 non-duplicate S. aureus isolates were collected from clinical samples and nasal swabs, from patients and healthcare workers (HCWs), between March 2016 and September 2016, at four teaching hospitals in Isfahan...
October 2017: Iranian Journal of Microbiology
Xingmei Liu, Shanshan Deng, Jinwei Huang, Yaling Huang, Yu Zhang, Qin Yan, Yanhong Wang, Yanyue Li, Chengfu Sun, Xu Jia
As an increasingly common cause of skin infections worldwide, the prevalence of antibiotic-resistant Staphylococcus aureus (S. aureus) across China has not been well documented. This literature aims to study the resistance profile to commonly used antibiotics, including macrolides, fusidic acid (FA) and mupirocin, and its relationship to the genetic typing in 34 S. aureus strains, including 6 methicillin-resistant S. aureus (MRSA), isolated from a Chinese hospital. The MIC results showed 27 (79.4%), 1 (2.9%) and 6 (17...
August 29, 2017: Oncotarget
Dua'a Jarajreh, Amin Aqel, Hamed Alzoubi, Wael Al-Zereini
INTRODUCTION: A high rate of infections with methicillin-resistant Staphylococcus aureus (MRSA) has been documented, in both hospital- (HA-MRSA) and community-acquired (CA-MRSA) diseases in Jordan. Erythromycin and clindamycin are considered treatments of choice. However, resistance to erythromycin with false susceptibility to clindamycin in vitro may lead to therapeutic failure. Hence, it is mandatory to study the prevalence of inducible resistance to macrolide-lincosamide-streptogramin B (iMLSB) antibiotics conferred by erm genes in those bacteria...
April 30, 2017: Journal of Infection in Developing Countries
Asma Mesbah Elkammoshi, Hamed Ghasemzadeh-Moghaddam, Syafinaz Amin Nordin, Niazlin Mohd Taib, Suresh Kumar Subbiah, Vasanthakumari Neela, Rukman Awang Hamat
BACKGROUND: Antibiotic resistance among Staphylococcus aureus is of great concern worldwide. This resistance is further complicated by the ability of S. aureus to confer cross-resistance to other antibiotics due to the presence of resistance genes, such as erythromycin resistance methylase (erm) genes, which render the bacterium resistant to macrolide-lincosamide-streptogramin B (MLSB) antibiotics. Resistance to these antibiotics can lead to therapeutic failure, resulting in significant morbidity and mortality in patients with S...
October 2016: Jundishapur Journal of Microbiology
Sareh Sadat Hosseini, Mohammad Niakan, Horieh Saderi, Mitra Motallebi, Morovat Taherikalani, Khairollah Asadollahi, Mohammad Emaneini
BACKGROUND AND OBJECTIVES: Macrolide, lincosamide and streptogramin type B (MLSB) antibiotics are important in the treatment of Staphylococcus aureus infections and existence of isolates with ability to resist against MLSB antibiotics is worrisome. MATERIALS AND METHODS: In this cross sectional study, 101 S. aureus isolates were collected from patients of five selected hospitals in Tehran over a period of five months. Disk diffusion tests and differentiation between constitutive and inducible resistances were carried out by D-test...
June 2016: Iranian Journal of Microbiology
A M Ammar, A M Attia, M I Abd El-Hamid, I M El-Shorbagy, S A Abd El-Kader
Antimicrobial resistance of methicillin-resistant Staphylococcus aureus (MRSA) poses a serious problem for clinicians worldwide. The present study attempted to evaluate the susceptibility patterns of MRSA to various antimicrobials and the prevalence of inducible clindamycin resistance as well as the relevant antibiotic and antiseptic resistance genes among these isolates. Totally, 40 MRSA isolates were recovered from examined milk and meat product samples (18.60%). Multi-drug resistance (MDR) was remarkably observed among 85% of these isolates...
August 31, 2016: Cellular and Molecular Biology
Carolina Y Gushiken, Liliane B Medeiros, Bruna P Correia, Joyce M Souza, Daniela V Moris, Valeria C Pereira, Rogerio Giuffrida, Marcus V P Rodrigues
Staphylococcus aureus can cause a variety of infections due to its high transmissibility, high pathogenic potential and resistance to multiple drugs, factors that contribute to the relevance of infections in healthcare services. The aim of this study was to document phenotypic and genotypic resistance factors of Staphylococcus aureus strains, isolated from nasal mucosa of medical students. A nasal swab was collected from the nares (nostrils) of 222 medical students. After collection, the samples were submitted to isolation and identification procedures...
September 2016: Anais da Academia Brasileira de Ciências
François Guérin, Christophe Isnard, Fiona Bucquet, Marguerite Fines-Guyon, Jean-Christophe Giard, Vincent Burrus, Vincent Cattoir
OBJECTIVES: The aim of the study was to identify the determinant responsible for erythromycin resistance in Helcococcus kunzii clinical isolate UCN99 and to characterize the genetic support and environment of this novel gene. METHODS: MICs were determined using the broth microdilution method according to EUCAST guidelines. The entire genome sequence of H. kunzii UCN99 was determined using a 454/Roche GS Junior sequencer. The fragment encompassing the new resistance gene and its own promoter was cloned into the pAT29 shuttle vector and the recombinant plasmid pAT29Ωerm(47) was expressed in both Staphylococcus aureus and Streptococcus agalactiae...
November 2016: Journal of Antimicrobial Chemotherapy
Gholamreza Goudarzi, Farzad Tahmasbi, Khatereh Anbari, Masoumeh Ghafarzadeh
BACKGROUND: Epidemiological data on antibiotic susceptibility of Staphylococcus strains isolated from nasal carriers in each region can be helpful to select appropriate drugs to eradicate carriage states, control nosocomial infections and also treat patients. OBJECTIVES: The current study aimed to investigate the antibiotic resistance profile and the molecular prevalence of the ermA, ermB, ermC and msrA genes among Staphylococcus strains isolated from the anterior nares of hospital employees...
February 2016: Iranian Red Crescent Medical Journal
Fahimeh Ghanbari, Hasan Ghajavand, Roholla Havaei, Mohammad-Saeid Jami, Farzad Khademi, Leila Heydari, Mojtaba Shahin, Seyed Asghar Havaei
BACKGROUND: The rising frequency of methicillin resistant Staphylococcus aureus (MRSA) has led to an increased use of antibiotics such as macrolide, lincosamide, streptogramin B (MLSB) for the treatment of S. aureus infections. Resistance to MLSB in S. aureus is commonly encoded by erm genes, which can be constitutive MLSB (cMLSB) or inducible MLSB (iMLSB). The purpose of this study was to determine the frequency of cMLSB, iMLSB, and MS phenotypes using D-test and polymerase chain reaction (PCR) methods...
2016: Advanced Biomedical Research
Jussyêgles Niedja da Paz Pereira, Marcelle Aquino Rabelo, Jailton Lobo da Costa Lima, Armando Monteiro Bezerra Neto, Ana Catarina de Souza Lopes, Maria Amélia Vieira Maciel
INTRODUCTION: There is a mechanism of macrolide resistance in Staphylococcus spp. which also affects the lincosamides and type B streptogramins characterizing the so-called MLSB resistance, whose expression can be constitutive (cMLSB) or inducible (iMLSB) and is encoded mainly by ermA and ermC genes. The cMLSB resistance is easily detected by susceptibility testing used in the laboratory routine, but iMLSB resistance is not. Therapy with clindamycin in cases of infection with isolated iMLSB resistance may fail...
May 2016: Brazilian Journal of Infectious Diseases
Heidar Rahimi, Habib Dastmalchi Saei, Malahat Ahmadi
BACKGROUND: Staphylococcus aureus is a significant pathogen that can colonize the nares of different animals, causing a wide range of infections in various hosts. OBJECTIVES: We intended to determine the prevalence of S. aureus in the nasal cavity of healthy ruminants and also to investigate the presence of antibiotic resistance genes. MATERIALS AND METHODS: In the present study, healthy cattle (n = 79), sheep (n = 78) and goats (n = 44) were screened for nasal carriage of S...
October 2015: Jundishapur Journal of Microbiology
Ameer Abbas, Preeti Srivastava, Prem Singh Nirwan
BACKGROUND: The increasing resistance to macrolide, lincosamide, streptogramin B (MLSB) agents among Staphylococcus aureus is becoming a challenge to microbiologist. Clindamycin has been a useful drug for treatment of infection caused by the staphylococcus aureus, but change in clindamycin sensitivity pattern due to various mechanisms is leading to therapeutic failure. One of the important mechanisms is mediation of resistance by erm genes. Staphylococcus strains which have erm genes show inducible clindamycin resistance that cannot be determined by routine disk diffusion test resulting in treatment failure...
June 2015: Journal of Clinical and Diagnostic Research: JCDR
Longping Li, Weiwei Feng, Zhiping Zhang, Huping Xue, Xin Zhao
BACKGROUND: There are limited data available on macrolide-lincosamide-streptogramin (MLS) resistance of Staphylococcus aureus (S. aureus) and coagulase-negative staphylococci (CoNS) from bovine milk in China. To address this knowledge gap, MLS resistance was determined in 121 S. aureus and 97 CoNS isolates. Minimum inhibitory concentrations (MICs) of MLS antibiotics were determined by an agar dilution method, while differentiation of MLS phenotypes was performed by a double-disc diffusion test...
2015: BMC Veterinary Research
Mojtaba Moosavian, Saeed Shoja, Soodabeh Rostami, Maryam Torabipour, Zahra Farshadzadeh
BACKGROUND AND OBJECTIVES: Resistance to macrolide can be mediated by erm and msrA genes in Staphylococcus aureus. There are the evidences that show erm genes may be causative agent of inducible or constitutive resistance. The aim of this study was to investigate the incidence of inducible clindamycin resistance and determine the most frequency of erm and msrA genes among S. aureus isolates. MATERIALS AND METHODS: In this study a total of 124 non duplicated clinical isolates of S...
December 2014: Iranian Journal of Microbiology
María González-Domínguez, Cristina Seral, Carmen Potel, Lucía Constenla, Sonia Algarate, M José Gude, Maximiliano Álvarez, Francisco Javier Castillo
INTRODUCTION: MRSA population dynamics is undergoing significant changes, and for this reason it is important to know which clones are circulating in our nosocomial environment. MATERIALS AND METHODS: A total of 118 MRSA isolates were collected from clinical samples from patients with previous hospital or healthcare contact (named as hospital-onset MRSA (HO-MRSA)) during a one year period. Susceptibility testing was performed by disk diffusion and microdilution...
November 2015: Enfermedades Infecciosas y Microbiología Clínica
Fatma Aydeniz Ozansoy, Nural Cevahir, İlknur Kaleli
Staphylococcus aureus is one of the most common cause of both community and healthcare-associated infections. As staphylococci have developed resistance to various antibiotics, initially to penicillins then to methicillin and glycopeptides and have the ability to cause epidemics, they continue to be a major problem from past to present. Methicillin resistance gave rise to the use of alternative antibiotics such as macrolides, however worldwide development of macrolide resistance limited the use of these antibiotics...
January 2015: Mikrobiyoloji Bülteni
Ghaleb Adwan, Kamel Adwan, Naser Jarrar, Alaa Amleh
This study aimed to evaluate the relation between the phenotypic antibiotic susceptibility patterns and the antibiotic resistance genes and to investigate the prevalence of macrolide, lincosamides, streptogramin, aminoglycoside and tetracycline resistance genes among MRSA isolates. A total of 55 clinical MRSA isolates were included in this study, antibiotic resistance was conducted by Kirby-Bauer disk diffusion method, broth microdilution assay and multiplex PCR technique. Our results showed that there was no discordance between conventional susceptibility testing and gene detection by multiplex PCR assay...
January 2014: Roumanian Archives of Microbiology and Immunology
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