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macrolide resistances staphylococcus aureus PCR genes

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https://www.readbyqxmd.com/read/27609468/genetic-basis-of-resistance-waves-among-methicillin-resistant-staphylococcus-aureus-isolates-recovered-from-milk-and-meat-products-in-egypt
#1
A M Ammar, A M Attia, M I Abd El-Hamid, I M El-Shorbagy, S A Abd El-Kader
Antimicrobial resistance of methicillin-resistant Staphylococcus aureus (MRSA) poses a serious problem for clinicians worldwide. The present study attempted to evaluate the susceptibility patterns of MRSA to various antimicrobials and the prevalence of inducible clindamycin resistance as well as the relevant antibiotic and antiseptic resistance genes among these isolates. Totally, 40 MRSA isolates were recovered from examined milk and meat product samples (18.60%). Multi-drug resistance (MDR) was remarkably observed among 85% of these isolates...
2016: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/27556226/nasal-carriage-of-resistant-staphylococcus-aureus-in-a-medical-student-community
#2
Carolina Y Gushiken, Liliane B Medeiros, Bruna P Correia, Joyce M Souza, Daniela V Moris, Valeria C Pereira, Rogerio Giuffrida, Marcus V P Rodrigues
Staphylococcus aureus can cause a variety of infections due to its high transmissibility, high pathogenic potential and resistance to multiple drugs, factors that contribute to the relevance of infections in healthcare services. The aim of this study was to document phenotypic and genotypic resistance factors of Staphylococcus aureus strains, isolated from nasal mucosa of medical students. A nasal swab was collected from the nares (nostrils) of 222 medical students. After collection, the samples were submitted to isolation and identification procedures...
September 2016: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/27494920/novel-chromosome-encoded-erm-47-determinant-responsible-for-constitutive-mlsb-resistance-in-helcococcus-kunzii
#3
François Guérin, Christophe Isnard, Fiona Bucquet, Marguerite Fines-Guyon, Jean-Christophe Giard, Vincent Burrus, Vincent Cattoir
OBJECTIVES: The aim of the study was to identify the determinant responsible for erythromycin resistance in Helcococcus kunzii clinical isolate UCN99 and to characterize the genetic support and environment of this novel gene. METHODS: MICs were determined using the broth microdilution method according to EUCAST guidelines. The entire genome sequence of H. kunzii UCN99 was determined using a 454/Roche GS Junior sequencer. The fragment encompassing the new resistance gene and its own promoter was cloned into the pAT29 shuttle vector and the recombinant plasmid pAT29Ωerm(47) was expressed in both Staphylococcus aureus and Streptococcus agalactiae...
November 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27195143/distribution-of-genes-encoding-resistance-to-macrolides-among-staphylococci-isolated-from-the-nasal-cavity-of-hospital-employees-in-khorramabad-iran
#4
Gholamreza Goudarzi, Farzad Tahmasbi, Khatereh Anbari, Masoumeh Ghafarzadeh
BACKGROUND: Epidemiological data on antibiotic susceptibility of Staphylococcus strains isolated from nasal carriers in each region can be helpful to select appropriate drugs to eradicate carriage states, control nosocomial infections and also treat patients. OBJECTIVES: The current study aimed to investigate the antibiotic resistance profile and the molecular prevalence of the ermA, ermB, ermC and msrA genes among Staphylococcus strains isolated from the anterior nares of hospital employees...
February 2016: Iranian Red Crescent Medical Journal
https://www.readbyqxmd.com/read/27135031/distribution-of-erm-genes-among-staphylococcus-aureus-isolates-with-inducible-resistance-to-clindamycin-in-isfahan-iran
#5
Fahimeh Ghanbari, Hasan Ghajavand, Roholla Havaei, Mohammad-Saeid Jami, Farzad Khademi, Leila Heydari, Mojtaba Shahin, Seyed Asghar Havaei
BACKGROUND: The rising frequency of methicillin resistant Staphylococcus aureus (MRSA) has led to an increased use of antibiotics such as macrolide, lincosamide, streptogramin B (MLSB) for the treatment of S. aureus infections. Resistance to MLSB in S. aureus is commonly encoded by erm genes, which can be constitutive MLSB (cMLSB) or inducible MLSB (iMLSB). The purpose of this study was to determine the frequency of cMLSB, iMLSB, and MS phenotypes using D-test and polymerase chain reaction (PCR) methods...
2016: Advanced Biomedical Research
https://www.readbyqxmd.com/read/27094233/phenotypic-and-molecular-characterization-of-resistance-to-macrolides-lincosamides-and-type-b-streptogramin-of-clinical-isolates-of-staphylococcus-spp-of-a-university-hospital-in-recife-pernambuco-brazil
#6
Jussyêgles Niedja da Paz Pereira, Marcelle Aquino Rabelo, Jailton Lobo da Costa Lima, Armando Monteiro Bezerra Neto, Ana Catarina de Souza Lopes, Maria Amélia Vieira Maciel
INTRODUCTION: There is a mechanism of macrolide resistance in Staphylococcus spp. which also affects the lincosamides and type B streptogramins characterizing the so-called MLSB resistance, whose expression can be constitutive (cMLSB) or inducible (iMLSB) and is encoded mainly by ermA and ermC genes. The cMLSB resistance is easily detected by susceptibility testing used in the laboratory routine, but iMLSB resistance is not. Therapy with clindamycin in cases of infection with isolated iMLSB resistance may fail...
May 2016: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/26568802/nasal-carriage-of-staphylococcus-aureus-frequency-and-antibiotic-resistance-in-healthy-ruminants
#7
Heidar Rahimi, Habib Dastmalchi Saei, Malahat Ahmadi
BACKGROUND: Staphylococcus aureus is a significant pathogen that can colonize the nares of different animals, causing a wide range of infections in various hosts. OBJECTIVES: We intended to determine the prevalence of S. aureus in the nasal cavity of healthy ruminants and also to investigate the presence of antibiotic resistance genes. MATERIALS AND METHODS: In the present study, healthy cattle (n = 79), sheep (n = 78) and goats (n = 44) were screened for nasal carriage of S...
October 2015: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/26266120/prevalence-of-mlsb-resistance-and-observation-of-erm-a-erm-c-genes-at-a-tertiary-care-hospital
#8
Ameer Abbas, Preeti Srivastava, Prem Singh Nirwan
BACKGROUND: The increasing resistance to macrolide, lincosamide, streptogramin B (MLSB) agents among Staphylococcus aureus is becoming a challenge to microbiologist. Clindamycin has been a useful drug for treatment of infection caused by the staphylococcus aureus, but change in clindamycin sensitivity pattern due to various mechanisms is leading to therapeutic failure. One of the important mechanisms is mediation of resistance by erm genes. Staphylococcus strains which have erm genes show inducible clindamycin resistance that cannot be determined by routine disk diffusion test resulting in treatment failure...
June 2015: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/26209340/macrolide-lincosamide-streptogramin-resistance-phenotypes-and-genotypes-of-coagulase-positive-staphylococcus-aureus-and-coagulase-negative-staphylococcal-isolates-from-bovine-mastitis
#9
Longping Li, Weiwei Feng, Zhiping Zhang, Huping Xue, Xin Zhao
BACKGROUND: There are limited data available on macrolide-lincosamide-streptogramin (MLS) resistance of Staphylococcus aureus (S. aureus) and coagulase-negative staphylococci (CoNS) from bovine milk in China. To address this knowledge gap, MLS resistance was determined in 121 S. aureus and 97 CoNS isolates. Minimum inhibitory concentrations (MICs) of MLS antibiotics were determined by an agar dilution method, while differentiation of MLS phenotypes was performed by a double-disc diffusion test...
2015: BMC Veterinary Research
https://www.readbyqxmd.com/read/25926961/inducible-clindamycin-resistance-in-clinical-isolates-of-staphylococcus-aureus-due-to-erm-genes-iran
#10
Mojtaba Moosavian, Saeed Shoja, Soodabeh Rostami, Maryam Torabipour, Zahra Farshadzadeh
BACKGROUND AND OBJECTIVES: Resistance to macrolide can be mediated by erm and msrA genes in Staphylococcus aureus. There are the evidences that show erm genes may be causative agent of inducible or constitutive resistance. The aim of this study was to investigate the incidence of inducible clindamycin resistance and determine the most frequency of erm and msrA genes among S. aureus isolates. MATERIALS AND METHODS: In this study a total of 124 non duplicated clinical isolates of S...
December 2014: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/25749415/antimicrobial-resistance-virulence-factors-and-genetic-lineages-of-hospital-onset-methicillin-resistant-staphylococcus-aureus-isolates-detected-in-a-hospital-in-zaragoza
#11
María González-Domínguez, Cristina Seral, Carmen Potel, Lucía Constenla, Sonia Algarate, M José Gude, Maximiliano Álvarez, Francisco Javier Castillo
INTRODUCTION: MRSA population dynamics is undergoing significant changes, and for this reason it is important to know which clones are circulating in our nosocomial environment. MATERIALS AND METHODS: A total of 118 MRSA isolates were collected from clinical samples from patients with previous hospital or healthcare contact (named as hospital-onset MRSA (HO-MRSA)) during a one year period. Susceptibility testing was performed by disk diffusion and microdilution...
November 2015: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/25706726/-investigation-of-macrolide-lincosamide-and-streptogramin-b-resistance-in-staphylococcus-aureus-strains-isolated-from-clinical-samples-by-phenotypical-and-genotypical-methods
#12
Fatma Aydeniz Ozansoy, Nural Cevahir, İlknur Kaleli
Staphylococcus aureus is one of the most common cause of both community and healthcare-associated infections. As staphylococci have developed resistance to various antibiotics, initially to penicillins then to methicillin and glycopeptides and have the ability to cause epidemics, they continue to be a major problem from past to present. Methicillin resistance gave rise to the use of alternative antibiotics such as macrolides, however worldwide development of macrolide resistance limited the use of these antibiotics...
January 2015: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/25518565/molecular-detection-of-nine-antibiotic-resistance-genes-in-methicillin-resistant-staphylococcus-aureus-isolates
#13
Ghaleb Adwan, Kamel Adwan, Naser Jarrar, Alaa Amleh
This study aimed to evaluate the relation between the phenotypic antibiotic susceptibility patterns and the antibiotic resistance genes and to investigate the prevalence of macrolide, lincosamides, streptogramin, aminoglycoside and tetracycline resistance genes among MRSA isolates. A total of 55 clinical MRSA isolates were included in this study, antibiotic resistance was conducted by Kirby-Bauer disk diffusion method, broth microdilution assay and multiplex PCR technique. Our results showed that there was no discordance between conventional susceptibility testing and gene detection by multiplex PCR assay...
January 2014: Roumanian Archives of Microbiology and Immunology
https://www.readbyqxmd.com/read/25223381/antimicrobial-susceptibility-and-resistance-mechanisms-of-methicillin-resistant-staphylococcus-aureus-isolated-from-12-hospitals-in-turkey
#14
Ömer Yıldız, Ahmet Yılmaz Çoban, Aslı Gamze Şener, Seher Ayten Coşkuner, Gülçin Bayramoğlu, Hüseyin Güdücüoğlu, Mustafa Özyurt, Müşerref Tatman-Otkun, Nihal Karabiber, Nuri Özkütük, Orhan Aktepe, Serkan Öncü, Uğur Arslan, Bülent Bozdoğan
INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important nosocomial pathogens and is also emerging in Turkish hospitals. The aim of this study was to determine the antimicrobial susceptibility profiles of MRSA isolated from Turkish hospitals. MATERIALS AND METHODS: A total of 397 MRSA strains isolated from 12 hospitals in Turkey were included to present study. Antimicrobial susceptibilities were tested using agar dilution method...
2014: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/25112954/prevalence-of-macrolide-lincosamide-and-streptogramin-resistance-among-staphylococci-in-a-tertiary-care-hospital-in-athens-greece
#15
N Vallianou, A Evangelopoulos, M Hadjisoteriou, A Avlami, G Petrikkos
The aims of the present study were to evaluate erythromycin, clindamycin, and streptogramin resistance rates, as well as the phenotypic and genotypic characteristics of erythromycin-resistant staphylococci in a Greek University Hospital. Macrolide, lincosamide, and streptogramin B-type resistance was investigated by double disk diffusion and the D-zone testing, while Minimal inhibitory concentration determination was performed among 656 erythromycin-resistant staphylococcal clinical consecutive isolates, too...
2015: Journal of Chemotherapy
https://www.readbyqxmd.com/read/24909489/emergence-of-sccmec-type-iii-with-variable-antimicrobial-resistance-profiles-and-spa-types-among-methicillin-resistant-staphylococcus-aureus-isolated-from-healthcare-and-community-acquired-infections-in-the-west-of-iran
#16
Sattar Mohammadi, Zamberi Sekawi, Azam Monjezi, Mohammad-Hossein Maleki, Setareh Soroush, Nourkhoda Sadeghifard, Iraj Pakzad, Farid Azizi-Jalilian, Mohammad Emaneini, Khairollah Asadollahi, Fazel Pourahmad, Raffaele Zarrilli, Morovat Taherikalani
INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen of public health importance. The prevalence of MRSA and its antimicrobial resistance pattern, as well as SCCmec and spa types, remain unclear both in the community and in the hospitals of the western region of Iran. METHODS: One hundred MRSA isolates were collected from different hospitals in the west of Iran during 2010-2011. Antimicrobial susceptibility testing to 15 antimicrobial agents was carried out by disk agar diffusion (DAD) method in accordance with the Clinical and Laboratory Standards Institute guidelines...
August 2014: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/24461550/tylosin-susceptibility-of-staphylococci-from-bovine-mastitis
#17
Monika Entorf, Andrea T Feßler, Kristina Kadlec, Heike Kaspar, Joachim Mankertz, Thomas Peters, Stefan Schwarz
Although the 16-membered macrolide tylosin is commonly used for the treatment of bovine mastitis, little information is currently available about the susceptibility of mastitis pathogens to tylosin. In the present study, 112 Staphylococcus aureus and 110 coagulase-negative Staphylococcus (CoNS) spp. isolates from cases of bovine mastitis were tested by broth microdilution and agar disk diffusion with 30 μg tylosin disks. Susceptibility to erythromycin was tested by broth microdilution and disk diffusion using 15 μg disks...
July 16, 2014: Veterinary Microbiology
https://www.readbyqxmd.com/read/24399388/prevalence-of-inducible-clindamycin-resistance-among-community-associated-staphylococcal-isolates-in-central-serbia
#18
A D Aleksandra, M S Misic, Z V Mira, N M Violeta, I T Dragana, B M Zoran, V S Dejan, S D Milanko, B D Dejan
The emergence of resistance to most antimicrobial agents in staphylococci indicates the need for new effective agents in the treatment of staphylococcal infections. Clindamycin is considered to be one safe, effective and less costly agent. We analysed 482 staphylococcal isolates. Detection of inducible clindamycin resistance was performed by the D-test, while the presence of methylases genes: erm (A), erm (B) and erm (C), as well as, macrolide efflux gene mef was determined by polymerase chain reaction. Inducible clindamycin resistance phenotype was significantly higher in Staphylococcus aureus (S...
January 2014: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/24260092/clonal-complex-398-methicillin-susceptible-staphylococcus-aureus-a-frequent-unspecialized-human-pathogen-with-specific-phenotypic-and-genotypic-characteristics
#19
Tomasz Chroboczek, Sandrine Boisset, Jean-Philippe Rasigade, Anne Tristan, Michele Bes, Helene Meugnier, François Vandenesch, Jerome Etienne, Frederic Laurent
Clonal complex 398 livestok-associated-MRSA (CC398 LA-MRSA) clone is described as a major animal pathogen that can also colonize and infect humans. CC398 methicillin susceptible Staphylococcus aureus (CC398 MSSA) is less described. We identified 126 CC398 MSSA strains of human origin within 6380 S. aureus isolates gathered between 2009 and 2011, from the French National Reference Centre for Staphylococci. They were characterized using antimicrobial susceptibility testing, spa typing, DNA microarrays (Identibac S...
2013: PloS One
https://www.readbyqxmd.com/read/24205917/emergence-of-methicillin-resistance-and-panton-valentine-leukocidin-positivity-in-hospital-and-community-acquired-staphylococcus-aureus-infections-in-beira-mozambique
#20
MULTICENTER STUDY
Birgitta T van der Meeren, Peter S Millard, Marco Scacchetti, Mirjam H Hermans, Mirrian Hilbink, Timótio B Concelho, Josefo J Ferro, Peter C Wever
OBJECTIVES: The objective of this study was to investigate the antibiotic resistance patterns, including methicillin resistance, inducible macrolide-lincosamide-streptogramin B (MLSB ) resistance and Panton-Valentine leukocidin (PVL) toxin gene carriage among hospital-acquired Staphylococcus aureus (HA-SA) and community-acquired S. aureus (CA-SA), in Beira, Mozambique. METHODS: In 2010-2011, two prospective surveillance studies were conducted on post-operative and burn wound infections at the Central Hospital of Beira and on skin and soft tissue abscesses at the São Lucas Health Centre...
February 2014: Tropical Medicine & International Health: TM & IH
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