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macrolides resistance genes staphylococcus aureus

Juliette R K Wipf, Matthew C Riley, Stephen A Kania, David A Bemis, Sabrina Andreis, Sybille Schwendener, Vincent Perreten
Whole-genome sequencing of Staphylococcus xylosus strain JW2311 from bovine mastitis milk identified the novel 49.3 kb macrolide-lincosamide-streptogramin B (MLSB) resistance plasmid pJW2311. It contained the macrolide resistance gene mph(C), the macrolide-streptogramin B resistance gene msr(A), and the new MLSB resistance gene erm (48) and could be transformed into S. aureus by electroporation. Functionality of erm (48) was demonstrated by cloning and expression in S. aureus.
April 24, 2017: Antimicrobial Agents and Chemotherapy
Ebrima Bojang, James Jafali, Vincent Perreten, John Hart, Emma M Harding-Esch, Ansumana Sillah, David C W Mabey, Martin J Holland, Robin L Bailey, Anna Roca, Sarah E Burr
BACKGROUND: Mass drug administration (MDA) with azithromycin is a corner-stone of trachoma control however it may drive the emergence of antimicrobial resistance. In a cluster-randomized trial (Clinical trial gov NCT00792922), we compared the reduction in the prevalence of active trachoma in communities that received three annual rounds of MDA to that in communities that received a single treatment round. We used the framework of this trial to carry out an opportunistic study to investigate if the increased rounds of treatment resulted in increased prevalence of nasopharyngeal carriage of macrolide-resistant Staphylococcus aureus...
March 28, 2017: BMC Microbiology
Carina Andrea Boncompain, Cristian Alejandro Suárez, Héctor Ricardo Morbidoni
Staphylococcus aureus causes numerous mild to severe infections in humans, both in health facilities and in the community. Patients and health care workers (HCWs) may disseminate strains during regular medical examinations or hospitalization. The aim of this study was to determine the nasal carriage rate of methicillin-susceptible and methicillin-resistant S. aureus among health care workers at Hospital Provincial del Centenario, a public general hospital in Rosario, Argentina. A transversal study was conducted on 320 health care workers...
March 24, 2017: Revista Argentina de Microbiología
S Vandendriessche, H De Boeck, A Deplano, M-F Phoba, O Lunguya, D Falay, N Dauly, J Verhaegen, O Denis, J Jacobs
Staphylococcus aureus is known worldwide as an invasive pathogen, but information on S. aureus from bloodstream infections in Central Africa remains scarce. A collection of S. aureus blood culture isolates recovered from hospitals in four provinces in the Democratic Republic of the Congo (2009-2013) was assessed. A total of 27/108 isolates were methicillin-resistant S. aureus (MRSA), of which >70% were co-resistant to aminoglycosides, tetracyclines, macrolides and lincosamides. For MRSA and methicillin-susceptible S...
January 23, 2017: European Journal of Clinical Microbiology & Infectious Diseases
S R Tintino, C D M Oliveira-Tintino, F F Campina, M S Costa, R P Cruz, R L S Pereira, J C Andrade, E O Sousa, J P Siqueira-Junior, H D M Coutinho, T C Leal-Balbino, V Q Balbino
The aim of this study is to evaluate the effect of ergosterol on steroids and cholesterol efflux pumps in multidrug resistant strains of S. aureus. Were used RN4220 harboring plasmid pUL5054, which carries the gene encoding the MsrA macrolide efflux protein; and IS-58, which possesses the TetK tetracycline efflux protein; 1199B resists hydrophilic fluoroquinolones via a NorA-mediated mechanism and wild strain 1199B. The Minimal Inhibitory Concentration (MIC) was determined and the evaluation of possible inhibition of efflux pumps by reduction of MIC...
March 2017: Microbial Pathogenesis
Raina Tzvetanova Gergova, Guergana Petrova, Stefan Gergov, Petko Minchev, Ivan Mitov, Tanya Strateva
BACKGROUND: Across the globe, upper respiratory tract infections (URTIs) are the most prevalent cause of morbidity in childhood. AIMS: The aim of our study is to analyze the incidence and etiology of bacterial URTIs in Bulgarian children, as well as the increasing antimicrobial resistance to the most common etiologic agents over a period of 17 years. STUDY DESIGN: Retrospective study. METHODS: The study material comprised the data from 4768 patients (aged 1-16 years) with URTI during the period from 1998-2014...
November 2016: Balkan Medical Journal
Asma Mesbah Elkammoshi, Hamed Ghasemzadeh-Moghaddam, Syafinaz Amin Nordin, Niazlin Mohd Taib, Suresh Kumar Subbiah, Vasanthakumari Neela, Rukman Awang Hamat
BACKGROUND: Antibiotic resistance among Staphylococcus aureus is of great concern worldwide. This resistance is further complicated by the ability of S. aureus to confer cross-resistance to other antibiotics due to the presence of resistance genes, such as erythromycin resistance methylase (erm) genes, which render the bacterium resistant to macrolide-lincosamide-streptogramin B (MLSB) antibiotics. Resistance to these antibiotics can lead to therapeutic failure, resulting in significant morbidity and mortality in patients with S...
October 2016: Jundishapur Journal of Microbiology
Sareh Sadat Hosseini, Mohammad Niakan, Horieh Saderi, Mitra Motallebi, Morovat Taherikalani, Khairollah Asadollahi, Mohammad Emaneini
BACKGROUND AND OBJECTIVES: Macrolide, lincosamide and streptogramin type B (MLSB) antibiotics are important in the treatment of Staphylococcus aureus infections and existence of isolates with ability to resist against MLSB antibiotics is worrisome. MATERIALS AND METHODS: In this cross sectional study, 101 S. aureus isolates were collected from patients of five selected hospitals in Tehran over a period of five months. Disk diffusion tests and differentiation between constitutive and inducible resistances were carried out by D-test...
June 2016: Iranian Journal of Microbiology
Kamelia Osman, Jihan Badr, Khalid S Al-Maary, Ihab M I Moussa, Ashgan M Hessain, Zeinab M S Amin Girah, Usama H Abo-Shama, Ahmed Orabi, Aalaa Saad
The use of antibiotics in farm management (growing crops and raising animals) has become a major area of concern. Its implications is the consequent emergence of antibiotic resistant bacteria (ARB) and accordingly their access into the human food chain with passage of antibiotic resistance genes (ARG) to the normal human intestinal microbiota and hence to other pathogenic bacteria causative human disease. Therefore, we pursued in this study to unravel the frequency and the quinolone resistance determining region, mecA and cfr genes of methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant S...
2016: Frontiers in Microbiology
Christian Strauss, Yanmin Hu, Anthony Coates, Vincent Perreten
A novel erm(44) gene variant, erm(44)v, has been identified by whole-genome sequencing in a Staphylococcus saprophyticus isolate from the skin of a healthy person. It has the particularity to confer resistance to macrolides and lincosamides but not to streptogramin B when expressed in S. aureus The erm(44)v gene resides on a 19,400-bp genomic island which contains phage-associated proteins and is integrated into the chromosome of S. saprophyticus.
January 2017: Antimicrobial Agents and Chemotherapy
A M Ammar, A M Attia, M I Abd El-Hamid, I M El-Shorbagy, S A Abd El-Kader
Antimicrobial resistance of methicillin-resistant Staphylococcus aureus (MRSA) poses a serious problem for clinicians worldwide. The present study attempted to evaluate the susceptibility patterns of MRSA to various antimicrobials and the prevalence of inducible clindamycin resistance as well as the relevant antibiotic and antiseptic resistance genes among these isolates. Totally, 40 MRSA isolates were recovered from examined milk and meat product samples (18.60%). Multi-drug resistance (MDR) was remarkably observed among 85% of these isolates...
August 31, 2016: Cellular and Molecular Biology
Xiao-Qin Mu, Bin-Bin Liu, Ephraim Hui, William Huang, Li-Chen Yao, Li-Bo Duo, Wen-Ying Sun, Gui-Qiu Li, Fu-Xiang Wang, Shu-Lin Liu
Macrolide-streptogramin type B resistance (the MSB phenotype) is a multidrug resistance phenotype in Staphylococcus aureus conferred by the resistance gene msrA. However, bacteria having the MSB phenotype are susceptible to lincosamides and 16-membered ring macrolides, which makes profiling resistance genes necessary and urgent for timely and appropriate use of antimicrobials. In this study, the loop-mediated isothermal amplification (LAMP) assay was optimized for prompt detection of the msrA gene. msrA gene sequences were obtained from the National Center for Biotechnology Information (NCBI) database and primers were designed using the LAMP primer designing software PrimerExplorer v...
December 2016: Journal of Global Antimicrobial Resistance
Carolina Y Gushiken, Liliane B Medeiros, Bruna P Correia, Joyce M Souza, Daniela V Moris, Valeria C Pereira, Rogerio Giuffrida, Marcus V P Rodrigues
Staphylococcus aureus can cause a variety of infections due to its high transmissibility, high pathogenic potential and resistance to multiple drugs, factors that contribute to the relevance of infections in healthcare services. The aim of this study was to document phenotypic and genotypic resistance factors of Staphylococcus aureus strains, isolated from nasal mucosa of medical students. A nasal swab was collected from the nares (nostrils) of 222 medical students. After collection, the samples were submitted to isolation and identification procedures...
September 2016: Anais da Academia Brasileira de Ciências
J R Wipf, V Perreten
Twenty-two methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from various infected locations in domestic cats and dogs between June 2008 and September 2014 were analyzed for their genotype, genetic fingerprint, virulence and antibiotic resistance profile. Eighteen strains belonged to the clonal complex (CC) 22 [ST22(MLST)-A(PFGE)-t032(spa)-IV(SCCmec) and ST22-A-t1214-IV], 2 strains to the livestock associated MRSA ST398-t011-IV and two were individual strains of ST5-t002-II and ST1-t001-IV...
June 2016: Schweizer Archiv Für Tierheilkunde
François Guérin, Christophe Isnard, Fiona Bucquet, Marguerite Fines-Guyon, Jean-Christophe Giard, Vincent Burrus, Vincent Cattoir
OBJECTIVES: The aim of the study was to identify the determinant responsible for erythromycin resistance in Helcococcus kunzii clinical isolate UCN99 and to characterize the genetic support and environment of this novel gene. METHODS: MICs were determined using the broth microdilution method according to EUCAST guidelines. The entire genome sequence of H. kunzii UCN99 was determined using a 454/Roche GS Junior sequencer. The fragment encompassing the new resistance gene and its own promoter was cloned into the pAT29 shuttle vector and the recombinant plasmid pAT29Ωerm(47) was expressed in both Staphylococcus aureus and Streptococcus agalactiae...
November 2016: Journal of Antimicrobial Chemotherapy
Andrea T Feßler, Qin Zhao, Sonja Schoenfelder, Kristina Kadlec, Geovana Brenner Michael, Yang Wang, Wilma Ziebuhr, Jianzhong Shen, Stefan Schwarz
The multiresistance plasmid pAFS11, obtained from a bovine methicillin-resistant Staphylococcus aureus (MRSA) isolate, was completely sequenced and analysed for its structure and organisation. Moreover, the susceptibility to the heavy metals cadmium and copper was determined by broth macrodilution. The 49,189-bp plasmid harboured the apramycin resistance gene apmA, two copies of the macrolide/lincosamide/streptogramin B resistance gene erm(B) (both located on remnants of a truncated transposon Tn917), the kanamycin/neomycin resistance gene aadD, the tetracycline resistance gene tet(L) and the trimethoprim resistance gene dfrK...
July 11, 2016: Veterinary Microbiology
Patrick Eberechi Akpaka, Rashida Roberts, Stefan Monecke
Staphylococcus aureus continues to pose major public health challenges in many areas because of antibiotic resistance problems. In the Caribbean, especially Trinidad and Tobago, the challenge is not different. This study was performed to evaluate the antimicrobial resistance gene prevalence among S. aureus isolates in Trinidad and Tobago. Standard and molecular microbiological methods, including the Microscan automated system, DNA microarray and multi locus sequence typing (MLST) analysis, were performed on 309 clinical S...
June 18, 2016: Journal of Infection and Public Health
Britta Kraushaar, Britta Ballhausen, Daniel Leeser, Bernd-Alois Tenhagen, Annemarie Käsbohrer, Alexandra Fetsch
Little is known about the characteristics of MRSA occurring along the broiler and turkey production chains. The aim of this study was to characterise and compare MRSA of turkey and broiler origin sampled on different production levels using a DNA microarray and antimicrobial susceptibility testing. Main differences could be observed in the prevalence of the resistance genes erm(C), aacA-aphD and tet(K) and the number of non-wild type strains with minimum inhibitory concentration values (MICs) above the epidemiological cut-off values (ECOFFs) for gentamicin and kanamycin...
June 1, 2016: Veterinary Microbiology
Sofia Santos Costa, Cláudia Palma, Kristina Kadlec, Andrea T Fessler, Miguel Viveiros, José Melo-Cristino, Stefan Schwarz, Isabel Couto
Plasmids play a key role in the genetic plasticity and survival of Staphylococcus aureus in challenging environments. Although many S. aureus plasmids have been described, still few studies portray the plasmid content of a given S. aureus population. The aim of this work was to characterize the plasmids carried by a collection of 53 S. aureus isolates collected in a large hospital in Lisbon, Portugal, and investigate their role in conferring resistance to several antimicrobial agents. Plasmids were present in 44 out of the 53 isolates and were grouped into eleven AccI restriction profiles...
December 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Saulo R Tintino, Cícera D Morais-Tintino, Fábia F Campina, Raimundo L Pereira, Maria do S Costa, Maria Flaviana B M Braga, Paulo W Limaverde, Jacqueline C Andrade, José P Siqueira-Junior, Henrique Douglas Melo Coutinho, Valdir Q Balbino, Tereza C Leal-Balbino, Jaime Ribeiro-Filho, Lucindo J Quintans-Júnior
Alpha-tocopherol is one the most abundant and biologically active isoforms of vitamin E. This compound is a potent antioxidant and one of most studied isoforms of vitamin E. Vitamin D3 (cholecalciferol) is an important nutrient for calcium homeostasis and bone health, that has also been recognized as a potent modulator of the immune response. Methicillin-resistant Staphylococcus aureus (MRSA) is the most important causative agent of both nosocomial and community-acquired infections. The aim of this study was to evaluate the inhibitory effect of alpha-tocopherol and cholecalciferol on both S...
2016: EXCLI journal
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