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macrolides resistance genes staphylococcus aureus

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https://www.readbyqxmd.com/read/27920760/prevalence-of-the-antibiotic-resistance-genes-in-coagulase-positive-and-negative-staphylococcus-in-chicken-meat-retailed-to-consumers
#1
Kamelia Osman, Jihan Badr, Khalid S Al-Maary, Ihab M I Moussa, Ashgan M Hessain, Zeinab M S Amin Girah, Usama H Abo-Shama, Ahmed Orabi, Aalaa Saad
The use of antibiotics in farm management (growing crops and raising animals) has become a major area of concern. Its implications is the consequent emergence of antibiotic resistant bacteria (ARB) and accordingly their access into the human food chain with passage of antibiotic resistance genes (ARG) to the normal human intestinal microbiota and hence to other pathogenic bacteria causative human disease. Therefore, we pursued in this study to unravel the frequency and the quinolone resistance determining region, mecA and cfr genes of methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant S...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27799208/a-novel-erm-44-gene-variant-from-a-human-staphylococcus-saprophyticus-confers-resistance-to-macrolides-lincosamides-but-not-streptogramins
#2
Christian Strauss, Yanmin Hu, Anthony Coates, Vincent Perreten
A novel erm (44) gene variant, erm (44)v, has been identified by whole genome sequencing in a Staphylococcus saprophyticus isolated from the skin of a healthy person. It has the particularity to confer resistance to macrolides and lincosamides, but not to streptogramins B when expressed in S. aureus The erm (44)v gene resides on a 19,400-bp genomic island which contains phage-associated proteins and is integrated into the chromosome of S. saprophyticus.
October 31, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27609468/genetic-basis-of-resistance-waves-among-methicillin-resistant-staphylococcus-aureus-isolates-recovered-from-milk-and-meat-products-in-egypt
#3
A M Ammar, A M Attia, M I Abd El-Hamid, I M El-Shorbagy, S A Abd El-Kader
Antimicrobial resistance of methicillin-resistant Staphylococcus aureus (MRSA) poses a serious problem for clinicians worldwide. The present study attempted to evaluate the susceptibility patterns of MRSA to various antimicrobials and the prevalence of inducible clindamycin resistance as well as the relevant antibiotic and antiseptic resistance genes among these isolates. Totally, 40 MRSA isolates were recovered from examined milk and meat product samples (18.60%). Multi-drug resistance (MDR) was remarkably observed among 85% of these isolates...
2016: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/27607914/a-rapid-loop-mediated-isothermal-amplification-lamp-method-for-detection-of-the-macrolide-streptogramin-type-b-resistance-gene-msra-in-staphylococcus-aureus
#4
Xiao-Qin Mu, Bin-Bin Liu, Ephraim Hui, William Huang, Li-Chen Yao, Li-Bo Duo, Wen-Ying Sun, Gui-Qiu Li, Fu-Xiang Wang, Shu-Lin Liu
Macrolide-streptogramin type B resistance (the MSB phenotype) is a multidrug resistance phenotype in Staphylococcus aureus conferred by the resistance gene msrA. However, bacteria having the MSB phenotype are susceptible to lincosamides and 16-membered ring macrolides, which makes profiling resistance genes necessary and urgent for timely and appropriate use of antimicrobials. In this study, the loop-mediated isothermal amplification (LAMP) assay was optimized for prompt detection of the msrA gene. msrA gene sequences were obtained from the National Center for Biotechnology Information (NCBI) database and primers were designed using the LAMP primer designing software PrimerExplorer v...
December 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27556226/nasal-carriage-of-resistant-staphylococcus-aureus-in-a-medical-student-community
#5
Carolina Y Gushiken, Liliane B Medeiros, Bruna P Correia, Joyce M Souza, Daniela V Moris, Valeria C Pereira, Rogerio Giuffrida, Marcus V P Rodrigues
Staphylococcus aureus can cause a variety of infections due to its high transmissibility, high pathogenic potential and resistance to multiple drugs, factors that contribute to the relevance of infections in healthcare services. The aim of this study was to document phenotypic and genotypic resistance factors of Staphylococcus aureus strains, isolated from nasal mucosa of medical students. A nasal swab was collected from the nares (nostrils) of 222 medical students. After collection, the samples were submitted to isolation and identification procedures...
September 2016: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/27504839/methicillin-resistant-staphylococcus-aureus-isolated-from-dogs-and-cats-in-switzerland
#6
J R Wipf, V Perreten
Twenty-two methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from various infected locations in domestic cats and dogs between June 2008 and September 2014 were analyzed for their genotype, genetic fingerprint, virulence and antibiotic resistance profile. Eighteen strains belonged to the clonal complex (CC) 22 [ST22(MLST)-A(PFGE)-t032(spa)-IV(SCCmec) and ST22-A-t1214-IV], 2 strains to the livestock associated MRSA ST398-t011-IV and two were individual strains of ST5-t002-II and ST1-t001-IV...
June 2016: Schweizer Archiv Für Tierheilkunde
https://www.readbyqxmd.com/read/27494920/novel-chromosome-encoded-erm-47-determinant-responsible-for-constitutive-mlsb-resistance-in-helcococcus-kunzii
#7
François Guérin, Christophe Isnard, Fiona Bucquet, Marguerite Fines-Guyon, Jean-Christophe Giard, Vincent Burrus, Vincent Cattoir
OBJECTIVES: The aim of the study was to identify the determinant responsible for erythromycin resistance in Helcococcus kunzii clinical isolate UCN99 and to characterize the genetic support and environment of this novel gene. METHODS: MICs were determined using the broth microdilution method according to EUCAST guidelines. The entire genome sequence of H. kunzii UCN99 was determined using a 454/Roche GS Junior sequencer. The fragment encompassing the new resistance gene and its own promoter was cloned into the pAT29 shuttle vector and the recombinant plasmid pAT29Ωerm(47) was expressed in both Staphylococcus aureus and Streptococcus agalactiae...
November 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27476981/complete-sequence-of-a-plasmid-from-a-bovine-methicillin-resistant-staphylococcus-aureus-harbouring-a-novel-ica-like-gene-cluster-in-addition-to-antimicrobial-and-heavy-metal-resistance-genes
#8
Andrea T Feßler, Qin Zhao, Sonja Schoenfelder, Kristina Kadlec, Geovana Brenner Michael, Yang Wang, Wilma Ziebuhr, Jianzhong Shen, Stefan Schwarz
The multiresistance plasmid pAFS11, obtained from a bovine methicillin-resistant Staphylococcus aureus (MRSA) isolate, was completely sequenced and analysed for its structure and organisation. Moreover, the susceptibility to the heavy metals cadmium and copper was determined by broth macrodilution. The 49,189-bp plasmid harboured the apramycin resistance gene apmA, two copies of the macrolide/lincosamide/streptogramin B resistance gene erm(B) (both located on remnants of a truncated transposon Tn917), the kanamycin/neomycin resistance gene aadD, the tetracycline resistance gene tet(L) and the trimethoprim resistance gene dfrK...
July 11, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27328777/molecular-characterization-of-antimicrobial-resistance-genes-against-staphylococcus-aureus-isolates-from-trinidad-and-tobago
#9
Patrick Eberechi Akpaka, Rashida Roberts, Stefan Monecke
Staphylococcus aureus continues to pose major public health challenges in many areas because of antibiotic resistance problems. In the Caribbean, especially Trinidad and Tobago, the challenge is not different. This study was performed to evaluate the antimicrobial resistance gene prevalence among S. aureus isolates in Trinidad and Tobago. Standard and molecular microbiological methods, including the Microscan automated system, DNA microarray and multi locus sequence typing (MLST) analysis, were performed on 309 clinical S...
June 18, 2016: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/27318609/antimicrobial-resistances-and-virulence-markers-in-methicillin-resistant-staphylococcus-aureus-from-broiler-and-turkey-a-molecular-view-from-farm-to-fork
#10
Britta Kraushaar, Britta Ballhausen, Daniel Leeser, Bernd-Alois Tenhagen, Annemarie Käsbohrer, Alexandra Fetsch
Little is known about the characteristics of MRSA occurring along the broiler and turkey production chains. The aim of this study was to characterise and compare MRSA of turkey and broiler origin sampled on different production levels using a DNA microarray and antimicrobial susceptibility testing. Main differences could be observed in the prevalence of the resistance genes erm(C), aacA-aphD and tet(K) and the number of non-wild type strains with minimum inhibitory concentration values (MICs) above the epidemiological cut-off values (ECOFFs) for gentamicin and kanamycin...
June 1, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27309487/plasmid-borne-antimicrobial-resistance-of-staphylococcus-aureus-isolated-in-a-hospital-in-lisbon-portugal
#11
Sofia Santos Costa, Cláudia Palma, Kristina Kadlec, Andrea T Fessler, Miguel Viveiros, José Melo-Cristino, Stefan Schwarz, Isabel Couto
Plasmids play a key role in the genetic plasticity and survival of Staphylococcus aureus in challenging environments. Although many S. aureus plasmids have been described, still few studies portray the plasmid content of a given S. aureus population. The aim of this work was to characterize the plasmids carried by a collection of 53 S. aureus isolates collected in a large hospital in Lisbon, Portugal, and investigate their role in conferring resistance to several antimicrobial agents. Plasmids were present in 44 out of the 53 isolates and were grouped into eleven AccI restriction profiles...
December 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27298617/action-of-cholecalciferol-and-alpha-tocopherol-on-staphylococcus-aureus-efflux-pumps
#12
Saulo R Tintino, Cícera D Morais-Tintino, Fábia F Campina, Raimundo L Pereira, Maria do S Costa, Maria Flaviana B M Braga, Paulo W Limaverde, Jacqueline C Andrade, José P Siqueira-Junior, Henrique Douglas Melo Coutinho, Valdir Q Balbino, Tereza C Leal-Balbino, Jaime Ribeiro-Filho, Lucindo J Quintans-Júnior
Alpha-tocopherol is one the most abundant and biologically active isoforms of vitamin E. This compound is a potent antioxidant and one of most studied isoforms of vitamin E. Vitamin D3 (cholecalciferol) is an important nutrient for calcium homeostasis and bone health, that has also been recognized as a potent modulator of the immune response. Methicillin-resistant Staphylococcus aureus (MRSA) is the most important causative agent of both nosocomial and community-acquired infections. The aim of this study was to evaluate the inhibitory effect of alpha-tocopherol and cholecalciferol on both S...
2016: EXCLI journal
https://www.readbyqxmd.com/read/27195143/distribution-of-genes-encoding-resistance-to-macrolides-among-staphylococci-isolated-from-the-nasal-cavity-of-hospital-employees-in-khorramabad-iran
#13
Gholamreza Goudarzi, Farzad Tahmasbi, Khatereh Anbari, Masoumeh Ghafarzadeh
BACKGROUND: Epidemiological data on antibiotic susceptibility of Staphylococcus strains isolated from nasal carriers in each region can be helpful to select appropriate drugs to eradicate carriage states, control nosocomial infections and also treat patients. OBJECTIVES: The current study aimed to investigate the antibiotic resistance profile and the molecular prevalence of the ermA, ermB, ermC and msrA genes among Staphylococcus strains isolated from the anterior nares of hospital employees...
February 2016: Iranian Red Crescent Medical Journal
https://www.readbyqxmd.com/read/27135031/distribution-of-erm-genes-among-staphylococcus-aureus-isolates-with-inducible-resistance-to-clindamycin-in-isfahan-iran
#14
Fahimeh Ghanbari, Hasan Ghajavand, Roholla Havaei, Mohammad-Saeid Jami, Farzad Khademi, Leila Heydari, Mojtaba Shahin, Seyed Asghar Havaei
BACKGROUND: The rising frequency of methicillin resistant Staphylococcus aureus (MRSA) has led to an increased use of antibiotics such as macrolide, lincosamide, streptogramin B (MLSB) for the treatment of S. aureus infections. Resistance to MLSB in S. aureus is commonly encoded by erm genes, which can be constitutive MLSB (cMLSB) or inducible MLSB (iMLSB). The purpose of this study was to determine the frequency of cMLSB, iMLSB, and MS phenotypes using D-test and polymerase chain reaction (PCR) methods...
2016: Advanced Biomedical Research
https://www.readbyqxmd.com/read/27133306/genotypic-and-phenotypic-characterization-of-methicillin-resistant-staphylococcus-aureus-mrsa-clones-with-high-level-mupirocin-resistance
#15
María González-Domínguez, Cristina Seral, Carmen Potel, Yolanda Sáenz, Maximiliano Álvarez, Carmen Torres, Francisco Javier Castillo
A high proportion of methicillin-resistant Staphylococcus aureus isolates recovered in one year period showed high-level mupirocin-resistance (HLMUPR-MRSA) in our environment (27.2%). HLMUPR-MRSA isolates were mainly collected from skin and soft tissue samples, and diabetes was the main related comorbidity condition. These isolates were more frequently found in vascular surgery. HLMUPR-MRSA was more resistant to aminoglycosides than mupirocin-susceptible MRSA, linked to the presence of bifunctional and/or nucleotidyltransferase enzymes with/without macrolide resistance associated with the msr(A) gene...
June 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27094233/phenotypic-and-molecular-characterization-of-resistance-to-macrolides-lincosamides-and-type-b-streptogramin-of-clinical-isolates-of-staphylococcus-spp-of-a-university-hospital-in-recife-pernambuco-brazil
#16
Jussyêgles Niedja da Paz Pereira, Marcelle Aquino Rabelo, Jailton Lobo da Costa Lima, Armando Monteiro Bezerra Neto, Ana Catarina de Souza Lopes, Maria Amélia Vieira Maciel
INTRODUCTION: There is a mechanism of macrolide resistance in Staphylococcus spp. which also affects the lincosamides and type B streptogramins characterizing the so-called MLSB resistance, whose expression can be constitutive (cMLSB) or inducible (iMLSB) and is encoded mainly by ermA and ermC genes. The cMLSB resistance is easily detected by susceptibility testing used in the laboratory routine, but iMLSB resistance is not. Therapy with clindamycin in cases of infection with isolated iMLSB resistance may fail...
May 2016: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27045297/molecular-markers-of-antimicrobial-resistance-in-methicillin-resistant-staphylococcus-aureus-sccmec-iv-presenting-different-genetic-backgrounds
#17
Pricilla Dias Moura de Matos, Tamara Lopes Rocha de Oliveira, Fernanda Sampaio Cavalcante, Dennis Carvalho Ferreira, Natália Lopes Pontes Iorio, Eliezer Menezes Pereira, Raiane Cardoso Chamon, Kátia Regina Netto Dos Santos
Methicillin-resistant Staphylococcus aureus (MRSA) carrying SCCmec type IV has emerged in hospitals worldwide. The aim of this study was to evaluate phenotypic and molecular characteristics of antimicrobial resistance in MRSA SCCmec IV isolates, presenting different genetic backgrounds, isolated from hospitals in Rio de Janeiro. The antimicrobial resistance of 128 S. aureus type IV isolates from 11 hospitals was characterized by the disk diffusion test and minimum inhibitory concentration (MIC) test. Mutations in parC gene, which encodes ciprofloxacin resistance, and genes associated with macrolide-lincosamide-streptogramin B (MLSb) resistance were also investigated...
December 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27008373/the-prevalence-of-genotypes-that-determine-resistance-to-macrolides-lincosamides-and-streptogramins-b-compared-with-spiramycin-susceptibility-among-erythromycin-resistant-staphylococcus-epidermidis
#18
Marek Juda, Beata Chudzik-Rzad, Anna Malm
Coagulase-negative staphylococci, particularly Staphylococcus epidermidis, can be regarded as potential reservoirs of resistance genes for pathogenic strains, e.g., Staphylococcus aureus. The aim of this study was to assess the prevalence of different resistance phenotypes to macrolide, lincosamide, and streptogramins B (MLSB) antibiotics among erythromycin-resistant S. epidermidis, together with the evaluation of genes promoting the following different types of MLSB resistance:ermA, ermB, ermC,msrA, mphC, and linA/A'...
March 2016: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/26568802/nasal-carriage-of-staphylococcus-aureus-frequency-and-antibiotic-resistance-in-healthy-ruminants
#19
Heidar Rahimi, Habib Dastmalchi Saei, Malahat Ahmadi
BACKGROUND: Staphylococcus aureus is a significant pathogen that can colonize the nares of different animals, causing a wide range of infections in various hosts. OBJECTIVES: We intended to determine the prevalence of S. aureus in the nasal cavity of healthy ruminants and also to investigate the presence of antibiotic resistance genes. MATERIALS AND METHODS: In the present study, healthy cattle (n = 79), sheep (n = 78) and goats (n = 44) were screened for nasal carriage of S...
October 2015: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/26564641/-phenotype-and-genotype-of-antimicrobial-resistance-on-nasal-staphylococcus-aureus-isolates-from-healthy-people
#20
Xiaomei Yan, Xiaoxia Tao, Xiaojie Yu, Jun Yan, Jianzhong Zhang
OBJECTIVE: To investigate the antimicrobial susceptibility and molecular nature related to the resistance on macrolides from nasal Staphylococcus (S.) aureus isolates among healthy people. METHODS: A total of 100 S. aureus isolates collected from 2009 to 2011 were tested for antimicrobial susceptibility by E-test. Double disc test (D-test) was used to detect the inducible clindamycin resistance. All S. aureus isolates were characterized by spa typing. Macrolides resistance genes were detected and compared with isolates that were collected clinically or from the livestock...
June 2015: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
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