Jiahong Hu, Fangfang Yang, Chong Liu, Nengzhi Wang, Yinghan Xiao, Yujie Zhai, Xinru Wang, Ren Zhang, Lulu Gao, Mengli Xu, Jialu Wang, Zheng Liu, Songlin Huang, Wenfeng Liu, Yajing Hu, Feng Liu, Yuqi Guo, Liang Wang, Jing Yuan, Zhihong Zhang, Jun Chu
Brainbow is a genetic cell-labeling technique that allows random colorization of multiple cells and real-time visualization of cell fate within a tissue, providing valuable insights into understanding complex biological processes. However, fluorescent proteins (FPs) in Brainbow have distinct excitation spectra with peak difference greater than 35 nm, which requires sequential imaging under multiple excitations and thus leads to long acquisition times. In addition, they are not easily used together with other fluorophores due to severe spectral bleed-through...
April 1, 2024: Communications Biology