keyword
https://read.qxmd.com/read/38531421/rice-ins-3-p-synthase1-rino1-participates-in-embryonic-development-by-regulating-inositol-associated-changes-in-auxin-synthesis-and-its-distribution
#1
JOURNAL ARTICLE
Lujian Zhou, Yiqin Xiong, Muhammad-Asad-Ullah Asad, Xianyue Guan, Yan Zhang, Da Su, Gang Pan, Fangmin Cheng
The breeding of low phytic acid (LPA) crops is widely considered an effective strategy to improve crop nutrition, but the LPA crops usually have inferior seed germination performance. To clarify the reason for the suboptimal seed performance of LPA rice, this study investigated the impact of reduced seed phytic acid (InsP6 ) content in rice ins(3)P synthase1 (EC 5.5.1.4, RINO1), one of the key targets for engineering LPA rice, knockouton cellular differentiation in seed embryos and its relation to myo-inositol metabolism and auxin signalling during embryogenesis...
2024: Physiologia Plantarum
https://read.qxmd.com/read/38522519/minor-electrostatic-changes-robustly-increase-vp40-membrane-binding-assembly-and-budding-of-ebola-virus-matrix-protein-derived-virus-like-particles
#2
JOURNAL ARTICLE
Balindile B Motsa, Tej Sharma, Michael Ciofi, Prem P Chapagain, Robert V Stahelin
Ebola virus (EBOV) is a filamentous negative-sense RNA virus which causes severe hemorrhagic fever. There are limited vaccines or therapeutics for prevention and treatment of EBOV, so it is important to get a detailed understanding of the virus lifecycle to illuminate new drug targets. EBOV encodes for the matrix protein, VP40, which regulates assembly and budding of new virions from the inner leaflet of the host cell plasma membrane (PM). In this work we determine the effects of VP40 mutations altering electrostatics on PM interactions and subsequent budding...
March 22, 2024: Journal of Biological Chemistry
https://read.qxmd.com/read/38514901/inpp5e-regulates-the-distribution-of-phospholipids-on-cilia-in-rpe1-cells
#3
JOURNAL ARTICLE
Denghui Zhai, Lamei Li, Cheng Chen, Xue Wang, Ruming Liu, Ying Shan
BACKGROUND: Primary cilia are static microtubule-based structures protruding from the cell surface and present on most vertebrate cells. The appropriate localization of phospholipids is essential for cilia formation and stability. INPP5E is a cilia-localized inositol 5-phosphatase; its deletion alters the phosphoinositide composition in the ciliary membrane, disrupting ciliary function. METHODS: The EGFP-2xP4MSidM , PHPLCδ1 -EGFP, and SMO-tRFP plasmids were constructed by the Gateway system to establish a stable RPE1 cell line...
March 21, 2024: Journal of Clinical Laboratory Analysis
https://read.qxmd.com/read/38509107/structure-of-an-open-k-atp-channel-reveals-tandem-pip-2-binding-sites-mediating-the-kir6-2-and-sur1-regulatory-interface
#4
JOURNAL ARTICLE
Camden M Driggers, Yi-Ying Kuo, Phillip Zhu, Assmaa ElSheikh, Show-Ling Shyng
ATP-sensitive potassium (KATP ) channels, composed of four pore-lining Kir6.2 subunits and four regulatory sulfonylurea receptor 1 (SUR1) subunits, control insulin secretion in pancreatic β-cells. KATP channel opening is stimulated by PIP2 and inhibited by ATP. Mutations that increase channel opening by PIP2 reduce ATP inhibition and cause neonatal diabetes. Although considerable evidence has implicated a role for PIP2 in KATP channel function, previously solved open-channel structures have lacked bound PIP2 , and mechanisms by which PIP2 regulates KATP channels remain unresolved...
March 20, 2024: Nature Communications
https://read.qxmd.com/read/38481533/spontaneous-signal-generation-by-an-excitable-system-for-cell-migration
#5
REVIEW
Satomi Matsuoka, Koji Iwamoto, Da Young Shin, Masahiro Ueda
Eukaryotic cells exhibit random migration in the absence of extracellular directional cues. This random migration acts as basal motility for various migratory responses such as chemotaxis. The self-organization of random motility requires the internal signals that determine the anterior side of the migrating cell be generated spontaneously from the intrinsic activities of intracellular signaling networks. Recent studies have identified an excitable system as the mechanism of the spontaneous signal generation...
2024: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/38474363/lamin-a-c-and-pi-4-5-p2-a-novel-complex-in-the-cell-nucleus
#6
JOURNAL ARTICLE
Sara Escudeiro-Lopes, Vlada V Filimonenko, Lenka Jarolimová, Pavel Hozák
Lamins, the nuclear intermediate filaments, are important regulators of nuclear structural integrity as well as nuclear functional processes such as DNA transcription, replication and repair, and epigenetic regulations. A portion of phosphorylated lamin A/C localizes to the nuclear interior in interphase, forming a lamin A/C pool with specific properties and distinct functions. Nucleoplasmic lamin A/C molecular functions are mainly dependent on its binding partners; therefore, revealing new interactions could give us new clues on the lamin A/C mechanism of action...
February 25, 2024: Cells
https://read.qxmd.com/read/38465747/a-binding-site-for-phosphoinositides-described-by-multiscale-simulations-explains-their-modulation-of-voltage-gated-sodium-channels
#7
JOURNAL ARTICLE
Yiechang Lin, Elaine Tao, James P Champion, Ben Corry
Voltage-gated sodium channels (Naᵥ) are membrane proteins which open to facilitate the inward flux of sodium ions into excitable cells. In response to stimuli, Naᵥ channels transition from the resting, closed state to an open, conductive state, before rapidly inactivating. Dysregulation of this functional cycle due to mutations causes diseases including epilepsy, pain conditions, and cardiac disorders, making Naᵥ channels a significant pharmacological target. Phosphoinositides are important lipid cofactors for ion channel function...
March 11, 2024: ELife
https://read.qxmd.com/read/38446032/the-er-tether-vapa-is-required-for-proper-cell-motility-and-anchors-er-pm-contact-sites-to-focal-adhesions
#8
JOURNAL ARTICLE
Hugo Siegfried, Georges Farkouh, Rémi Le Borgne, Catherine Pioche-Durieu, Thaïs De Azevedo Laplace, Agathe Verraes, Lucien Daunas, Jean-Marc Verbavatz, Mélina L Heuzé
Cell motility processes highly depend on the membrane distribution of Phosphoinositides, giving rise to cytoskeleton reshaping and membrane trafficking events. Membrane contact sites serve as platforms for direct lipid exchange and calcium fluxes between two organelles. Here, we show that VAPA, an ER transmembrane contact site tether, plays a crucial role during cell motility. CaCo2 adenocarcinoma epithelial cells depleted for VAPA exhibit several collective and individual motility defects, disorganized actin cytoskeleton and altered protrusive activity...
March 6, 2024: ELife
https://read.qxmd.com/read/38438249/pip5k1%C3%AE-regulates-axon-formation-by-limiting-rap1-activity
#9
JOURNAL ARTICLE
Danila Di Meo, Trisha Kundu, Priyadarshini Ravindran, Bhavin Shah, Andreas W Püschel
During their differentiation, neurons establish a highly polarized morphology by forming axons and dendrites. Cortical and hippocampal neurons initially extend several short neurites that all have the potential to become an axon. One of these neurites is then selected as the axon by a combination of positive and negative feedback signals that promote axon formation and prevent the remaining neurites from developing into axons. Here, we show that Pip5k1γ is required for the formation of a single axon as a negative feedback signal that regulates C3G and Rap1 through the generation of phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2 )...
May 2024: Life Science Alliance
https://read.qxmd.com/read/38360201/role-of-phosphatidic-acid-lipids-on-plasma-membrane-association-of-the-ebola-virus-matrix-protein-vp40
#10
JOURNAL ARTICLE
Michael D Cioffi, Monica L Husby, Bernard S Gerstman, Robert V Stahelin, Prem P Chapagain
The Ebola virus matrix protein VP40 is responsible for the formation of the viral matrix by localizing at the inner leaflet of the human plasma membrane (PM). Various lipid types, including PI(4,5)P2 (i.e. PIP2 ) and phosphatidylserine (PS), play active roles in this process. Specifically, the negatively charged headgroups of both PIP2 and PS interact with the basic residues of VP40 and stabilize it at the membrane surface, allowing for eventual egress. Phosphatidic acid (PA), resulting from the enzyme phospholipase D (PLD), is also known to play an active role in viral development...
February 13, 2024: Biochimica et Biophysica Acta. Molecular and Cell Biology of Lipids
https://read.qxmd.com/read/38358281/the-phosphatidylinositol-5-phosphatase-synaptojanin1-limits-integrin-mediated-invasion-of-staphylococcus-aureus
#11
JOURNAL ARTICLE
Yong Shi, Petra Muenzner, Stefanie Schanz-Jurinka, Christof R Hauck
The gram-positive bacterium Staphylococcus aureus can invade non-professional phagocytic cells by associating with the plasma protein fibronectin to exploit host cell integrins. Integrin-mediated internalization of these pathogens is facilitated by the local production of phosphatidylinositol-4,5-bisphosphate (PI-4,5-P2 ) via an integrin-associated isoform of phosphatidylinositol-5' kinase. In this study, we addressed the role of PI-4,5-P2 -directed phosphatases on internalization of S. aureus . ShRNA-mediated knockdown of individual phosphoinositide 5-phosphatases revealed that synaptojanin1 (SYNJ1) is counteracting invasion of S...
February 15, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38334661/evidence-for-involvement-of-adp-ribosylation-factor-6-in-intracellular-trafficking-and-release-of-murine-leukemia-virus-gag
#12
JOURNAL ARTICLE
Hyokyun Kang, Taekwon Kang, Lauryn Jackson, Amaiya Murphy, Takayuki Nitta
Murine leukemia viruses (MuLVs) are simple retroviruses that cause several diseases in mice. Retroviruses encode three basic genes: gag , pol , and env. Gag is translated as a polyprotein and moves to assembly sites where viral particles are shaped by cleavage of poly-Gag. Viral release depends on the intracellular trafficking of viral proteins, which is determined by both viral and cellular factors. ADP-ribosylation factor 6 (Arf6) is a small GTPase that regulates vesicular trafficking and recycling of different types of cargo in cells...
January 31, 2024: Cells
https://read.qxmd.com/read/38310114/phosphoglycerate-kinase-1-acts-as-a-cargo-adaptor-to-promote-egfr-transport-to-the-lysosome
#13
JOURNAL ARTICLE
Shao-Ling Chu, Jia-Rong Huang, Yu-Tzu Chang, Shu-Yun Yao, Jia-Shu Yang, Victor W Hsu, Jia-Wei Hsu
The epidermal growth factor receptor (EGFR) plays important roles in multiple cellular events, including growth, differentiation, and motility. A major mechanism of downregulating EGFR function involves its endocytic transport to the lysosome. Sorting of proteins into intracellular pathways involves cargo adaptors recognizing sorting signals on cargo proteins. A dileucine-based sorting signal has been identified previously for the sorting of endosomal EGFR to the lysosome, but a cargo adaptor that recognizes this signal remains unknown...
February 3, 2024: Nature Communications
https://read.qxmd.com/read/38295986/pi-4-5-p-2-binding-sites-in-the-ebola-virus-matrix-protein-vp40-modulate-assembly-and-budding
#14
JOURNAL ARTICLE
Kristen A Johnson, Melissa R Budicini, Nisha Bhattarai, Tej Sharma, Sarah Urata, Bernard S Gerstman, Prem P Chapagain, Sheng Li, Robert V Stahelin
Ebolavirus (EBOV) causes severe hemorrhagic fever in humans and is lethal in a large percentage of those infected. The EBOV matrix protein VP40 is a peripheral binding protein that forms a shell beneath the lipid bilayer in virions and virus-like particles (VLPs). VP40 is required for virus assembly and budding from the host cell plasma membrane. VP40 is a dimer that can rearrange into oligomers at the plasma membrane interface, but it is unclear how these structures form and how they are stabilized. We therefore investigated the ability of VP40 to form stable oligomers using in vitro and cellular assays...
January 29, 2024: Journal of Lipid Research
https://read.qxmd.com/read/38294155/controlling-physical-and-biochemical-parameters-of-actin-nucleation-using-a-patterned-model-lipid-membrane
#15
JOURNAL ARTICLE
Yosuke Yamazaki, Yuuri Miyata, Kenichi Morigaki, Makito Miyazaki
Self-assembly of nanoscale actin cytoskeletal proteins into filamentous networks requires organizing actin nucleation areas on the plasma membrane through recruiting actin nucleators and nucleation-promoting factors (NPFs) to the areas. To investigate impacts of the nucleation geometry on actin network assembly, we localized NPF or nucleator on defined micropatterns of laterally mobile lipid bilayers confined in a framework of a polymerized lipid bilayer. We demonstrated that actin network assembly in purified protein mixtures was confined on NPF- or nucleator-localized fluid bilayers...
January 31, 2024: Nano Letters
https://read.qxmd.com/read/38252473/disulfide-bridge-dependent-dimerization-triggers-fgf2-membrane-translocation-into-the-extracellular-space
#16
JOURNAL ARTICLE
Fabio Lolicato, Julia P Steringer, Roberto Saleppico, Daniel Beyer, Jaime Fernandez-Sobaberas, Sebastian Unger, Steffen Klein, Petra Riegerová, Sabine Wegehingel, Hans-Michael Müller, Xiao J Schmitt, Shreyas Kaptan, Christian Freund, Martin Hof, Radek Šachl, Petr Chlanda, Ilpo Vattulainen, Walter Nickel
Fibroblast growth factor 2 (FGF2) exits cells by direct translocation across the plasma membrane, a type I pathway of unconventional protein secretion. This process is initiated by phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2 )-dependent formation of highly dynamic FGF2 oligomers at the inner plasma membrane leaflet, inducing the formation of lipidic membrane pores. Cell surface heparan sulfate chains linked to glypican-1 (GPC1) capture FGF2 at the outer plasma membrane leaflet, completing FGF2 membrane translocation into the extracellular space...
January 22, 2024: ELife
https://read.qxmd.com/read/38239314/a-novel-interaction-between-chemokine-and-phosphoinositide-signaling-in-metastatic-prostate-cancer
#17
JOURNAL ARTICLE
Codrut Radoiu, Barani Govindarajan, Michael Wang, Diego Sbrissa, Michael L Cher, Sreenivasa R Chinni
Prostate cancer commonly metastasizes to bone due to its favorable microenvironment for cell growth and survival. Currently, the standard of care for metastatic prostate cancer is medical castration in conjunction with chemotherapeutic agents and newer anti-androgen/androgen receptor therapies. While these therapies aim to improve the quality of life in patients with advanced disease, resistance to these therapies is inevitable prompting the development of newer therapies to contain disease progression. The CXCL12/CXCR4 axis has previously been shown to be involved in prostate cancer cell homing to bone tissue, and new investigations found a novel interaction of Phosphatidyl Inositol 4 kinase IIIa (PI4KA) downstream of chemokine signaling...
July 2023: Medical Research Archives
https://read.qxmd.com/read/38182888/the%C3%A2-stx17-snap47-vamp7-vamp8-complex-is-the-default-snare-complex-mediating-autophagosome-lysosome-fusion
#18
JOURNAL ARTICLE
Fenglei Jian, Shen Wang, Rui Tian, Yufen Wang, Chuangpeng Li, Yan Li, Shixuan Wang, Chao Fang, Cong Ma, Yueguang Rong
Autophagosome-lysosome fusion mediated by SNARE complexes is an essential step in autophagy. Two SNAP29-containing SNARE complexes have been extensively studied in starvation-induced bulk autophagy, while the relevant SNARE complexes in other types of autophagy occurring under non-starvation conditions have been overlooked. Here, we found that autophagosome-lysosome fusion in selective autophagy under non-starvation conditions does not require SNAP29-containing SNARE complexes, but requires the STX17-SNAP47-VAMP7/VAMP8 SNARE complex...
January 5, 2024: Cell Research
https://read.qxmd.com/read/38156113/lipid-kinases-pip5ks-and-pip4ks-potential-drug-targets-for-breast-cancer
#19
REVIEW
Yue Jin, Jian Xue
Phosphoinositides, a small group of lipids found in all cellular membranes, have recently garnered heightened attention due to their crucial roles in diverse biological processes and different diseases. Among these, phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), the most abundant bis-phosphorylated phosphoinositide within the signaling system, stands notably connected to breast cancer. Not only does it serve as a key activator of the frequently altered phosphatidylinositol 3-kinase (PI3K) pathway in breast cancer, but also its conversion to phosphatidylinositol-3,4,5-triphosphate (PI(3,4,5)P3) is an important direction for breast cancer research...
2023: Frontiers in Oncology
https://read.qxmd.com/read/38141770/actin-binding-protein-profilin1-is-an-important-determinant-of-cellular-phosphoinositide-control
#20
JOURNAL ARTICLE
Morgan M C Ricci, Andrew Orenberg, Lee Ohayon, David Gau, Rachel C Wills, Yongho Bae, Tuhin Das, David Koes, Gerald R V Hammond, Partha Roy
Membrane poly-phosphoinositides (PPIs) are lipid-signaling molecules that undergo metabolic turnover and influence a diverse range of cellular functions. PPIs regulate the activity and/or spatial localization of a number of actin-binding proteins (ABPs) through direct interactions; however, it is much less clear whether ABPs could also be an integral part in regulating PPI signaling. In this study, we show that ABP profilin1 (Pfn1) is an important molecular determinant of cellular content of PI(4,5)P2 (the most abundant PPI in cells)...
December 21, 2023: Journal of Biological Chemistry
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