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Ayşe Dündar, Abdulkadir Şengün, Canan Başlak, Mahmut Kuş
OBJECTIVES: The aim of this in vitro study was to evaluate the effects of citric acid containing fluoride, nano-hydroxyapatite, and casein on eroded enamel. DESIGN: The crowns of 120 extracted bovine incisors were embedded in acrylic resin. An enamel window (2 × 3 mm) was created on the surface. Before in vitro pellicle formation samples were eroded in 1% citric acid (pH = 3.2) for 1 h at 36 °C and were randomly classified to eight groups (n = 15) as follows: Positive control: 1% citric acid, Negative control: Distilled water, F1: 0...
June 15, 2018: Archives of Oral Biology
Nelma Cristina Silva Pacheco, Ana Paula Carli de Almeida, Kariny Cássia de Siqueira, Faena Moura de Lima, Silvia Regina de Lima Reis, Marcia Queiroz Latorraca, Luiz Fabrizio Stoppiglia
Nutritional recovery of early malnutrition with soybean diet reduces liver glycogen stores in the fed state and produces liver insulin resistance. We investigated whether nutritional recovery on a soybean flour diet alters hepatic gluconeogenesis in the adult offspring of rats deprived of protein during pregnancy and lactation. Male rats from mothers that were fed either 17% (C) or 6% (L) protein during pregnancy and lactation were maintained on a 17% casein (CC, n=16 and LC, n=17), 17% soybean flour (CS, n=10 and LS, n=10) or 6% casein (LL, n=10) diet after weaning...
June 22, 2018: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
Shichao Ding, Zhiling Li, Yuan Cheng, Chunbao Du, Junfeng Gao, Yong-Wei Zhang, Nan Zhang, Zhaotong Li, Ninghui Chang, Xiaoling Hu
In order to facilitate the broad applications of molecular recognition materials in biomedical areas, it is critical to enhance their adsorption capacity while maintaining their excellent recognition performance. In this work, we designed and synthesized well-defined peptide-imprinted mesoporous silica (PIMS) for specific recognition of an immunostimulating hexapeptide from human casein (IHHC) by using amphiphilic ionic liquid as the surfactant to anchor IHHC via a combination of one step sol-gel method and docking oriented imprinting approach...
June 21, 2018: Nanotechnology
Rui Lu, Ting Zhang, Daijin Wu, Zhengyi He, Lei Jiang, Minya Zhou, Yong Cheng
Human copper/zinc superoxide dismutase (CuZn-SOD) and extracellular superoxide dismutase (EC-SOD) are two superoxide dismutases that scavenge reactive oxygen species (ROS). Their biological role of eliminating oxidative stress caused by excessive ROS levels in living organisms has been utilized in medical treatment, preventing skin photoaging and food preservation. In this study, we employed two sequences that encode human CuZn-SOD and EC-SOD, along with goat beta-casein 5' and 3' regulatory elements, to construct mammary gland-specific expression vectors...
June 20, 2018: Transgenic Research
Guowei Shu, Xiaoyu Shi, Li Chen, Jianbo Kou, Jiangpeng Meng, He Chen
Antioxidant peptides are currently the focus of many studies, since they eliminate free radicals in the human body without harmful effects. In the present study, Lactobacillus casei L61 was used as a starter culture to ferment goat milk because of its high capacity to produce antioxidant peptides. An optimal nutrients formula (casein, casein peptone, glucose, soybean peptone, inulin, calcium lactate, and cysteine) was investigated by Plackett⁻Burman (P⁻B) and Box⁻Behnken (B⁻B) designs for response surface methodology (RSM)...
June 20, 2018: Nutrients
Kentaro Furukawa, Tomoyuki Fukuda, Shun-Ichi Yamashita, Tetsu Saigusa, Yusuke Kurihara, Yutaka Yoshida, Hiromi Kirisako, Hitoshi Nakatogawa, Tomotake Kanki
Mitophagy plays an important role in mitochondrial quality control. In yeast, phosphorylation of the mitophagy receptor Atg32 by casein kinase 2 (CK2) upon induction of mitophagy is a prerequisite for interaction of Atg32 with Atg11 (an adaptor protein for selective autophagy) and following delivery of mitochondria to the vacuole for degradation. Because CK2 is constitutively active, Atg32 phosphorylation must be precisely regulated to prevent unrequired mitophagy. We found that the PP2A (protein phosphatase 2A)-like protein phosphatase Ppg1 was essential for dephosphorylation of Atg32 and inhibited mitophagy...
June 19, 2018: Cell Reports
Michele Faccia, Giuseppe Gambacorta, Giovanni Martemucci, Giuseppe Natrella, Angela G D'Alessandro
The work described in this Research Communication addressed the hypothesis that it is possible to produce cheese from donkey milk by appropriate adjusting of the cheesemaking parameters. A series of coagulation trials were performed on donkey milk, alone or fortified with goat milk (85/15 and 70/30, v/v), using calf rennet under different technological conditions. The parameters that changed were pH and concentration of soluble calcium, amount of rennet added and temperature of coagulation. Donkey milk gave rise to sufficiently firm curd only at 'extreme' technological conditions and, as expected, addition of goat milk improved coagulation...
June 20, 2018: Journal of Dairy Research
Elisabeth Schlögl, Mariya Y Radeva, Franziska Vielmuth, Camilla Schinner, Jens Waschke, Volker Spindler
Pemphigus vulgaris (PV) is a potentially lethal autoimmune disease characterized by blister formation of the skin and mucous membranes and is caused by autoantibodies against desmoglein (Dsg) 1 and Dsg3. Dsg1 and Dsg3 are linked to keratin filaments in desmosomes, adhering junctions abundant in tissues exposed to high levels of mechanical stress. The binding of the autoantibodies leads to internalization of Dsg3 and a collapse of the keratin cytoskeleton-yet, the relevance and interdependence of these changes for loss of cell-cell adhesion and blistering is poorly understood...
2018: Frontiers in Immunology
Leandro José de Assis, Mevlut Ulas, Laure Nicolas Annick Ries, Nadia Ali Mohamed El Ramli, Ozlem Sarikaya-Bayram, Gerhard H Braus, Ozgur Bayram, Gustavo Henrique Goldman
The attachment of one or more ubiquitin molecules by SCF (<u>S</u>kp-<u>C</u>ullin-<u>F</u>-box) complexes to protein substrates targets them for subsequent degradation by the 26S proteasome, allowing the control of numerous cellular processes. Glucose-mediated signaling and subsequent carbon catabolite repression (CCR) are processes relying on the functional regulation of target proteins, ultimately controlling the utilization of this carbon source. In the filamentous fungus Aspergillus nidulans , CCR is mediated by the transcription factor CreA, which modulates the expression of genes encoding biotechnologically relevant enzymes...
June 19, 2018: MBio
Bonnie A Lyon-Marion, Anjuliee M Mittelman, Justine Rayner, Daniele S Lantagne, Kurt D Pennell
Applying silver nanoparticles (nAg) or silver nitrate (AgNO3 ) to ceramic water filters improves microbiological efficacy, reduces biofilm formation, and protects stored water from recontamination. A challenge in ceramic filter production is adding sufficient silver to achieve these goals without exceeding the maximum recommended silver concentration in drinking water. Silver release is affected by silver type, application method, and influent water chemistry. Despite a lack of data, there is an assumption that chlorinated water should not be used as influent water because it may increase silver elution...
June 5, 2018: Water Research
Mu Chen, Dechun Yin, Shuai Guo, Dong-Zhu Xu, Zhuo Wang, Zhenhui Chen, Michael Rubart-von der Lohe, Shien-Fong Lin, Thomas H Everett, James N Weiss, Peng-Sheng Chen
KEY POINTS: It is unknown if sex difference exists in cardiac apamin sensitive small conductance Ca2+ activated K+ (SK) current (IKAS ). There is no sex difference in IKAS at basal condition. However, there is larger IKAS in female rabbit ventricles than in males during isoproterenol infusion. IKAS activation by isoproterenol leads to action potential triangulation in females, indicating its abundant activation at early phases of repolarization. IKAS activation in females induces negative Ca2+ -voltage coupling and promotes electromechanical discordant phase 2 repolarization alternans...
June 19, 2018: Journal of Physiology
Nan-Nan Wang, Liu-Yan Zhou, Ying-Xiu Li, Zong-Jun Du
A strictly aerobic, Gram-stain negative, long rod-shaped, motile by gliding and yellow pigmented bacterium, designated strain w01T , was isolated from marine sediment. The strain was characterised to determine its taxonomic position by using a polyphasic approach. Strain w01T was observed to grow optimally in the presence of 3.0% (w/v) NaCl, at 30 °C and to hydrolyse Tweens 20, 40 and 80, starch, casein and alginate. Carotenoid pigments were found to be produced but not flexirubin-type pigments. On the basis of 16S rRNA gene sequence similarities, strain w01T is phylogenetically affiliated with the genus Aquimarina and is closely related to Aquimarina macrocephali JCM 15542T (97...
June 18, 2018: Antonie Van Leeuwenhoek
Awatef Ouertani, Ines Chaabouni, Amor Mosbah, Justine Long, Mohamed Barakat, Pascal Mansuelle, Olfa Mghirbi, Afef Najjari, Hadda-Imene Ouzari, Ahmed S Masmoudi, Marc Maresca, Philippe Ortet, Didier Gigmes, Kamel Mabrouk, Ameur Cherif
Milk and dairy products harbor a wide variety of bacterial species that compete for both limited resources and space. Under these competitive conditions, bacteria develop specialized mechanisms to protect themselves during niche colonization and nutrient acquisition processes. The bacterial antagonism mechanisms include the production of antimicrobial agents or molecules that facilitate competitor dispersal. In the present work, a bacterial strain designated RC6 was isolated from Ricotta and identified as Bacillus cereus ...
2018: Frontiers in Microbiology
M Sannino, S Faugno, M Crimaldi, A Di Francia, L Ardito, F Serrapica, F Masucci
A study was carried out to evaluate the effects of an automatic milking system (AMS) on milk yield and composition of buffalo (Mediterranean-type Bubalus bubalis) cows. Performed from January 2015 to December 2015 in an organic buffalo dairy farm equipped with both a traditional tandem milking parlor and an AMS, the study involved 90 primiparous buffaloes randomly allotted to a tandem or AMS group from 5 to 10 d of lactation onward. Number of milkings per day and daily milk yield of each cow were recorded, and individual milk sampling was carried out twice a month...
June 13, 2018: Journal of Dairy Science
Ruihua Zhang, Xiaoyang Pang, Jing Lu, Lu Liu, Shuwen Zhang, Jiaping Lv
This work investigated the impact of high intensity ultrasound (HIUS) pretreatment on the functional properties and structural characteristics of micellar casein concentrate (MCC). Microfiltered casein protein retentates were treated with HIUS for 0.5, 1, 2, and 5 min prior to spray drying. The results showed that conductivity, solubility, emulsifying, gelling increased significantly as the ultrasonic time prolonged, but the change of pH value were insignificant. In addition, the structural characteristics of MCC for all samples were studied...
October 2018: Ultrasonics Sonochemistry
Hao Wang, Zhixin Tian
Analysis of phosphoproteins always faces the challenge of low stoichiometry, which demands highly selective and efficient enrichment in the initial sample preparation. Here we report our synthesis of the novel titanium (IV) ion immobilized adenosine triphosphate functionalized silica nanoparticles (Ti4+ -ATP-NPs) for efficient enrichment of intact phosphoproteins. The average diameter of Ti4+ -ATP-NPs was about 128 nm with good dispersibility and the saturated adsorption capacity for β-casein was 1046.5 mg/g...
June 6, 2018: Journal of Chromatography. A
Marc Teissier, Hélène Larroque, Christèle Robert-Granié
BACKGROUND: In 2017, genomic selection was implemented in French dairy goats using the single-step genomic best linear unbiased prediction (ssGBLUP) method, which assumes that all single nucleotide polymorphisms explain the same fraction of genetic variance. However, ssGBLUP is not suitable for protein content, which is controlled by a major gene, i.e. α s 1 casein. This gene explains about 40% of the genetic variation in protein content. In this study, we evaluated the accuracy of genomic prediction using different genomic methods to include the effect of the α s 1 casein gene...
June 15, 2018: Genetics, Selection, Evolution: GSE
Emmanuelle Gouot, Wajid Bhat, Anne Rufiange, Eric Fournier, Eric Paquet, Amine Nourani
CK2 is an essential protein kinase implicated in various cellular processes. In this study, we address a potential role of this kinase in chromatin modulations associated with transcription. We found that CK2 depletion from yeast cells leads to replication-independent increase of histone H3K56 acetylation and global activation of H3 turnover in coding regions. This suggests a positive role of CK2 in maintenance/recycling of the histone H3/H4 tetramers during transcription. Interestingly, strand-specific RNA-seq analyses show that CK2 inhibits global cryptic promoters driving both sense and antisense transcription...
June 14, 2018: Nucleic Acids Research
Yasunori Fukumoto, Kazuaki Takahashi, Noriyuki Suzuki, Yasumitsu Ogra, Yuji Nakayama, Naoto Yamaguchi
An interaction between the Rad17-RFC2-5 and 9-1-1 complexes is essential for ATR-Chk1 signaling, which is one of the major DNA damage checkpoints. Recently, we showed that the polyanionic C-terminal tail of human Rad17 and the embedded conserved sequence iVERGE are important for the interaction with 9-1-1 complex. Here, we show that Rad17-S667 in the C-terminal tail is constitutively phosphorylated in vivo in a casein kinase 2-dependent manner, and the phosphorylation is important for 9-1-1 interaction. The serine phosphorylation of Rad17 could be seen in the absence of exogenous genotoxic stress, and was mostly abolished by S667A substitution...
June 11, 2018: Biochemical and Biophysical Research Communications
D B Torres, A Lopes, A J Rodrigues, J J Cerqueira, N Sousa, J A R Gontijo, P A Boer
Animal evidence has suggested that maternal emotional and nutritional stress during pregnancy is associated with behavioral outcomes in offspring. The nature of the stresses applied may differ, but it is often assumed that the mother's hippocampus-hypothalamic-pituitary-adrenal (HHPA) axis response releases higher levels of glucocorticoid hormones. The bed nucleus of the stria terminalis (BNST) is in a pivotal position to regulate the HHPA axis and the stress response, and it has been implicated in anxiety behavior...
June 14, 2018: Journal of Developmental Origins of Health and Disease
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