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https://www.readbyqxmd.com/read/29655734/toward-water-solvation-of-rice-proteins-via-backbone-hybridization-by-casein
#1
Tao Wang, Ming Yue, Pengcheng Xu, Ren Wang, Zhengxing Chen
Water insolubility is one of the major bottlenecks restricting the commercial availability of such food proteins as rice proteins (RPs), zein, etc. Here, we report that the structural hybridization of RPs and casein in a mass ratio of 1:0.01 can boost the solubility of RPs to over 90%. A structural analysis demonstrated that the backbones of the RPs and casein were integrated in basic solution (pH 12.0) and folded together into higher structures with subsequent neutralization. The hybrid backbones obtained improved the molecules' resistance to structural changes during neutralization, and formed protein bodies with appreciable exposed polar groups, which may have been buried in the absence of casein...
August 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29648526/fluviicola-kyonggii-sp-nov-a-bacterium-isolated-from-forest-soil-and-emended-description-of-the-genus-fluviicola
#2
Ram Hari Dahal, Jaisoo Kim
A novel bacterium, designated strain CA-1T , was isolated from forest soil in Kyonggi University. Cells were strictly aerobic, Gram-stain-negative, catalase-positive, oxidase-negative, non-motile, non-spore-forming, rod-shaped and red-orange-pigmented. Strain CA-1T hydrolysed casein and DNA. It was able to grow at 15-37 °C, pH 5.5-9.0 and at 0-2 % (w/v) NaCl concentration. Flexirubin-type pigments were present. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that strain CA-1T formed a lineage within the family Crocinitomicaceae of the phylum Bacteroidetes that was distinct from Fluviicola hefeinensis MYL-8T (96...
April 12, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29644510/a-raman-spectroscopy-based-approach-for-detection-and-discrimination-of-streptococcus-thermophilus-and-lactobacillus-bulgaricus-phages-at-low-titer-in-raw-milk
#3
Emine Kübra Tayyarcan, Esra Acar Soykut, Ismail Hakki Boyaci
In this study, a method combining Raman spectroscopy with chemometric analysis was developed for detection of phage presence in raw milk and discrimination of Streptococcus thermophilus and Lactobacillus bulgaricus phages which are among the main phages causing problems in dairy industry. For this purpose, S. thermophilus and L. bulgaricus phages were added into raw milk separately, and then some pretreatments such as fat separation, removal of casein, and filtration were applied to the raw milk samples. Raman spectra of the samples were collected and then analyzed using principal component analysis in order to discriminate these phages in raw milk...
April 11, 2018: Folia Microbiologica
https://www.readbyqxmd.com/read/29644075/regulation-of-amino-acid-transporters-in-the-mammary-gland-from-late-pregnancy-to-peak-lactation-in-the-sow
#4
Fang Chen, Shihai Zhang, Zixiao Deng, Qiqi Zhou, Lin Cheng, Sung Woo Kim, Jun Chen, Wutai Guan
Background: Milk protein is crucial for milk quality in sows and health of newborn piglets. Plasma amino acids (AA) in sows are important precursors for milk protein synthesis in the mammary gland. In order to study the regulation of AA transported in sow mammary glands and possible underlying mechanisms, we measured the expression of genes coding for milk proteins, AA transporter expressions, and plasma AA concentrations in sows at three different physiological stages (D-17, D1 and D17 of lactation), and then further investigated the regulation of AA transport across the cell membrane by adaptive mechanisms using pig mammary epithelial cells (PMEC) as an in vitro model...
2018: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29627464/letter-to-the-editor
#5
Reza Khodarahmi
I read with great interest the paper by Dehvari and Ghahghaei (Dehvari and Ghahghaei, 2018 [1]). Their paper aimed to prove that biosynthesized AgNPs mediated by Pulicaria undulata L. has the capability in inhibiting amyloid fibril formation and thus could be considered as a therapeutic agent in the treatment of amyloidosis disorders. According to the literature (Jangholi et al., 2018 [2]), Thioflavin T (ThT) is a commonly used probe to monitor in vitro amyloid fibril formation. Also, since ThT fluorescence originates only from the bound population of the dye molecules, the several orders of magnitude increase in the fluorescence intensity makes ThT an unusually sensitive and efficient reporter...
April 5, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29627244/jugular-arginine-infusion-relieves-lipopolysaccharide-triggered-inflammatory-stress-and-improves-immunity-status-of-lactating-dairy-cows
#6
F F Zhao, T Y Wu, H R Wang, L Y Ding, Gulzar Ahmed, H W Li, W Tian, Y Z Shen
The objective of this study was to evaluate the effects of jugular l-Arg infusion on performance and immune function during lipopolysaccharide (LPS)-induced inflammation of lactating dairy cows. Eight Holstein cows (multiparous, 608.8 ± 31.5 kg) at mid-lactation were randomly assigned to 5-d jugular infusions of control (saline), Arg (3 g/h), LPS (0.033 μg/kg per h), and LPS + Arg (0.033 μg/kg per h of LPS and 3 g/h of Arg) in a replicated 4 × 4 Latin square design with 4 infusion periods separated by 10-d noninfusion periods...
April 4, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29622185/physicochemical-characterization-of-mineral-iron-zinc-bound-caseinate-and-their-mineral-uptake-in-caco-2-cells
#7
B G Shilpashree, Sumit Arora, Suman Kapila, Vivek Sharma
Milk proteins (especially caseins) are widely accepted as good vehicle for the delivery of various bioactive compounds including minerals. Succinylation is one of the most acceptable chemical modification techniques to enhance the mineral binding ability of caseins. Addition of minerals to succinylated proteins may alter their physicochemical and biochemical properties. Physicochemical characteristics of succinylated sodium caseinate (S.NaCN)-mineral (iron/zinc) complexes were elucidated. Chromatographic behaviour and fluorescence intensity confirmed the structural modification of S...
August 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29621630/conformational-properties-of-intrinsically-disordered-proteins-bound-to-the-surface-of-silica-nanoparticles
#8
Michele Vitali, Valentina Rigamonti, Antonino Natalello, Barbara Colzani, Svetlana Avvakumova, Stefania Brocca, Carlo Santambrogio, Joanna Narkiewicz, Giuseppe Legname, Miriam Colombo, Davide Prosperi, Rita Grandori
BACKGROUND: Protein-nanoparticle (NP) interactions dictate properties of nanoconjugates relevant for bionanotechnology. Non-covalent adsorption generates a protein corona (PC) formed by an inner and an outer layer, the hard and soft corona (HC, SC). Intrinsically disordered proteins (IDPs) exist in solution as conformational ensembles, whose features in the presence of NPs are not well known. METHODS: Three IDPs (α-casein, Sic1 and α-synuclein) and lysozyme are compared, describing conformational properties inside HC on silica NPs by circular dichroism (CD) and Fourier-transform infrared (FTIR) spectroscopy...
April 2, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29620268/induction-of-huh%C3%A2-7-cell-apoptosis-by-hcv-core-proteins-via-ck1%C3%AE-%C3%A2-p53%C3%A2-bid-signaling-pathway
#9
Shanshan Shen, Chunyang Li, Mingjia Dai, Xuebing Yan
Hepatitis C virus (HCV)‑infected liver cells sensitize host cells to tumor necrosis factor (TNF)‑related apoptosis‑inducing ligand (TRAIL)‑induced cell apoptosis; however, the precise mechanisms are unknown. In the present study, flow cytometry demonstrated that the Annexin V‑positive Huh‑7 cell number was higher in groups transfected with core proteins when compared with the pcDNA3.1 group. The mRNA and protein expression levels of B‑cell lymphoma 2 (Bcl‑2) were negatively associated, while Bcl‑2‑associated X protein (Bax) were positively correlated, with cell apoptotic rate, which, were verified by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blotting...
April 5, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29620052/inflammation-activates-the-cxcl16-pathway-to-contribute-to-tubulointerstitial-injury-in-diabetic-nephropathy
#10
Ze-Bo Hu, Kun-Ling Ma, Yang Zhang, Gui-Hua Wang, Liang Liu, Jian Lu, Pei-Pei Chen, Chen-Chen Lu, Bi-Cheng Liu
Inflammation and lipid disorders play crucial roles in synergistically accelerating the progression of diabetic nephropathy (DN). In this study we investigated how inflammation and lipid disorders caused tubulointerstitial injury in DN in vivo and in vitro. Diabetic db/db mice were injected with 10% casein (0.5 mL, sc) every other day for 8 weeks to cause chronic inflammation. Compared with db/db mice, casein-injected db/db mice showed exacerbated tubulointerstitial injury, evidenced by increased secretion of extracellular matrix (ECM) and cholesterol accumulation in tubulointerstitium, which was accompanied by activation of the CXC chemokine ligand 16 (CXCL16) pathway...
April 5, 2018: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/29619665/expression-and-purification-of-the-main-component-contained-in-camel-milk-and-its-antimicrobial-activities-against-bacterial-plant-pathogens
#11
Abbas Tanhaeian, Farajollah Shahriari Ahmadi, Mohammad Hadi Sekhavati, Mojtaba Mamarabadi
Lactoferrin is the most dominant protein in milk after casein. This protein plays a crucial role in many biological processes including the regulation of iron metabolism, induction and modulation of the immune system, the primary defense against microorganisms, inhibiting lipid peroxidation and presenting antimicrobial activity against various pathogens such as parasites, fungi, bacteria, and viruses. The major antimicrobial effect of lactoferrin is related to its N-terminal tail where different peptides for instance lactoferricin and lactoferrampin which are important for their antimicrobial abilities are present...
April 4, 2018: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29619377/roles-of-nucks1-in-diseases-susceptibility-potential-biomarker-and-regulatory-mechanisms
#12
REVIEW
Pengru Huang, Yujie Cai, Bin Zhao, Lili Cui
Nuclear casein kinase and cyclin-dependent kinase substrate 1 (NUCKS1) is a 27 kD chromosomal, highly conserved, and vertebrate-specific protein. NUCKS1 gene encodes a nuclear protein and the conserved regions of NUCKS1 contain several consensus phosphorylation sites for casein kinase II (CK2) and cyclin-dependent kinases (Cdk) and a basic DNA-binding domain. NUCKS1 is similar to the high mobility group (HMG) family which dominates chromatin remodeling and regulates gene transcription. Meanwhile, NUCKS1 is a RAD51 associated protein 1 (RAD51AP1) paralog that is significant for homologous recombination (HR) and genome stability and also a transcriptional regulator of the insulin signaling components...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29619237/refining-the-clinical-phenotype-of-okur-chung-neurodevelopmental-syndrome
#13
Moe Akahira-Azuma, Yoshinori Tsurusaki, Yumi Enomoto, Jun Mitsui, Kenji Kurosawa
We describe an 8-year-old Japanese boy with a de novo recurrent missense mutation in CSNK2A1 , c.593A>G, that is causative of Okur-Chung neurodevelopmental syndrome. He exhibited distinctive facial features, severe growth retardation with relative macrocephaly, and friendly, hyperactive behavior. His dysmorphic features might suggest a congenital histone modification defect syndrome, such as Kleefstra, Coffin-Siris, or Rubinstein-Taybi syndromes, which are indicative of functional interactions between the casein kinase II, alpha 1 gene and histone modification factors...
2018: Human Genome Variation
https://www.readbyqxmd.com/read/29616893/acidovorax-kalamii-sp-nov-isolated-from-a-water-sample-of-the-river-ganges
#14
Deepika Pal, Navjot Kaur, Sarabjeet Kour Sudan, Bhawana Bisht, Srinivasan Krishnamurthi, Shanmugam Mayilraj
A Gram-stain-negative, rod-shaped, aerobic, straw yellow, motile strain, designated KNDSW-TSA6T , belonging to the genus Acidovorax, was isolated from a water sample of the river Ganges, downstream of the city of Kanpur, Uttar Pradesh, India. Cells were aerobic, non-endospore-forming and motile with single polar flagella. It differed from its phylogenetically related strains by phenotypic characteristics such as hydrolysis of urea, gelatin, casein and DNA, and the catalase reaction. The major fatty acids were C16 : 1ω7c/C16 : 1ω6c, C16 : 0 and C18 : 1ω7c/C18 : 1ω6c...
April 4, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29616801/comparison-of-protein-hydrolysis-catalysed-by-bovine-porcine-or-human-trypsins
#15
Yuxi Deng, Harry Gruppen, Peter A Wierenga
Based on trypsin specificity (lysine, arginine) trypsins from different sources are expected to hydrolyse a given protein to the same theoretical maximum degree of hydrolysis (DHmax,theo). This is in contrast with experiments. This study aims to reveal if the difference in experimental DHmax (DHmax,exp) by bovine, porcine or human trypsins is due to their secondary specificity, using α-lactalbumin and β-casein. Peptide analysis showed that from all cleavage sites ~78% were efficiently hydrolysed by porcine trypsin, and ~47% and ~53% were efficiently hydrolysed by bovine and human trypsins, respectively...
April 4, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29611137/taxonomic-description-and-genome-sequence-of-halobacillus-marinus-sp-nov-a-novel-strain-isolated-from-chilika-lake-india
#16
Ananta N Panda, Samir Ranjan Mishra, Lopamudra Ray, Surajit Das, Gurdeep Rastogi, Ajit Kumar Pattanaik, Tapan Kumar Adhya, Mrutyunjay Suar, Vishakha Raina
moderately halophilic spore forming, motile, Gram-positive, rod-shaped bacterial strain designated as KGW1T was isolated from water sample of Chilika Lake and characterized taxonomically using polyphasic approach. The strain grew in the presence of 0-25% (w/v) NaCl in marine salt agar media, hydrolyzes casein, and gelatin and shows presence of alkaline proteases. The major cell wall menaquinone was MK7 and major cellular fatty acids were anteiso-C15:0 (44.89%), anteiso-C17:0 (6.18%), isoC15:0 (19.38%), and iso-C16:0 (7...
April 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/29606502/a-positive-feed-forward-loop-between-lncrna-cytor-and-wnt-%C3%AE-catenin-signaling-promotes-metastasis-of-colon-cancer
#17
Ben Yue, Chenchen Liu, Huimin Sun, Mengru Liu, Chenlong Song, Ran Cui, Shenglong Qiu, Ming Zhong
We previously demonstrated that long non-coding RNA cytoskeleton regulator RNA (CYTOR), also known as Linc00152, was significantly overexpressed in colon cancer and conferred resistance to oxaliplatin-induced apoptosis. At the same time, elevated CYTOR expression was also reported in gastric cancer and exerted influences on epithelial-mesenchymal transition (EMT) markers. However, the precise mechanism by which CYTOR promotes the EMT phenotype and cancer metastasis remains poorly understood. Here, we showed that loss of epithelial characteristics and simultaneous gain of mesenchymal features correlated with CYTOR expression...
March 6, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29606478/identification-of-a-novel-ace-inhibitory-peptide-from-casein-and-evaluation-of-the-inhibitory-mechanisms
#18
Maolin Tu, Cong Wang, Cheng Chen, Ruyi Zhang, Hanxiong Liu, Weihong Lu, Lianzhou Jiang, Ming Du
Various bioactive peptides are continuously being identified from casein hydrolysates. In this work, a novel angiotensin I-converting enzyme (ACE)-inhibitory (ACEI) peptide, NMAINPSKENLCSTFCK, derived from the αs2 -casein fragment residues 25-41, was screened and identified by UPLC-ESI-Q-TOF-MS/MS from tryptic casein hydrolysate. The IC50 value of the peptide, determined by an HPLC method, was 129.07 μM. The Lineweaver-Burk plot showed that this peptide acted as a mixed-type inhibitor against ACE, which might be attributed to the peptide being susceptible to degradation by ACE, indicating that the mixed-type inhibition could partly be a result of newly generated peptide fragments...
August 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29606442/purification-and-characterisation-of-a-protease-tamarillin-from-tamarillo-fruit
#19
Zhao Li, Ken Scott, Yacine Hemar, Huoming Zhang, Don Otter
A protease from tamarillo fruit (Cyphomandra betacea Cav.) was purified by ammonium sulphate precipitation and diethylaminoethyl-Sepharose chromatography. Protease activity was determined on selected peak fractions using a casein substrate. Sodium dodecyl sulphate polyacrylamide gel electrophoresis analysis showed that the peak with the highest protease activity consisted of one protein of molecular mass ca. 70 kDa. The protease showed optimal activity at pH 11 and 60 °C. It was sensitive to phenylmethylsulphonyl fluoride while ethylenediaminetetraacetic acid and p-chloromercuribenzoic acid had little effect on its activity, indicating that this enzyme was a serine protease...
August 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29606427/%C3%AE-and-%C3%AE-casein-aggregation-induced-by-riboflavin-sensitized-photo-oxidation-occurs-via-di-tyrosine-cross-links-and-is-oxygen-concentration-dependent
#20
E Fuentes-Lemus, E Silva, F Leinisch, E Dorta, L G Lorentzen, M J Davies, C López-Alarcón
Type I photo-oxidation generates Trp-(TrpN) and Tyr-derived (TyrO) radicals in proteins which can dimerize producing cross-links, or alternatively react with O2 . It was therefore hypothesized that the O2 concentration may have a significant effect on dye-photosensitized reactions. We studied photo-oxidation of α- and β-caseins induced by riboflavin (RF), a photosensitizing vitamin present in milk, under aerobic and anaerobic conditions. Triplet-state RF induced oxidative modifications on both caseins, and significant levels of cross-links...
August 1, 2018: Food Chemistry
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