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https://www.readbyqxmd.com/read/28092746/supranutritional-selenium-intake-from-enriched-milk-casein-impairs-hepatic-insulin-sensitivity-via-attenuated-irs-pi3k-akt-signaling-and-decreased-pgc-1%C3%AE-expression-in-male-sprague-dawley-rats
#1
Priska Stahel, Julie J Kim, Scott R L Cieslar, Jenny M Warrington, Changting Xiao, John P Cant
Selenium (Se)-enriched milk provides antioxidant benefits and has therapeutic potential against cancer. However, both antidiabetic and prodiabetic effects have been attributed to Se. Our objective was to evaluate the effect of Se-enriched milk casein on insulin sensitivity in rats when given at the requirement of 0.25 ppm Se and supranutritionally on both low- and high-fat diets. Two hundred sixteen male Sprague-Dawley rats were fed low- or high-fat diets containing one, two or eight times the Se requirement in a randomized block design...
January 7, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28092150/issues-in-nutrition-dietary-supplements
#2
Margaret E Thompson, Mary Barth Noel
The majority of American adults report use of one or more dietary supplements every day or occasionally. The Dietary Supplement Health and Education Act of 1994 defines dietary supplements and regulates their manufacture and distribution. One of the most commonly used supplements is vitamin D. Measurement of serum levels of vitamin D must be undertaken with the caveats that different laboratories define normal levels differently, and that there is rarely a clinical correlation with the actual level. Patients should understand that supplements should not be used to excess, as there are toxicities and other adverse effects associated with most of them...
January 2017: FP Essentials
https://www.readbyqxmd.com/read/28091716/rapid-concentration-detection-and-differentiation-of-bacteria-in-skimmed-milk-using-surface-enhanced-raman-scattering-mapping-on-4-mercaptophenylboronic-acid-functionalized-silver-dendrites
#3
Panxue Wang, Shintaro Pang, Brooke Pearson, Yayoi Chujo, Lynne McLandsborough, Mingtao Fan, Lili He
A novel method was developed to rapidly concentrate, detect, and differentiate bacteria in skimmed milk using surface enhanced Raman scattering (SERS) mapping on 4-mercaptophenylboronic acid (4-MPBA) functionalized silver (Ag) dendrites. The 4-MPBA functionalized Ag dendritic SERS substrate was used to capture the bacterial cells and enhance the bacterial signal. Salmonella, a significantly important food pathogen, was used as the representative strain to optimize and evaluate the developed method. The capture efficiency for Salmonella enterica subsp enterica BAA1045 (SE1045) was 84...
January 14, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28091360/collagen-peptide-ingestion-alters-lipid-metabolism-related-gene-expression-and-the-unfolded-protein-response-in-mouse-liver
#4
Chisa Tometsuka, Yoh-Ichi Koyama, Tomoko Ishijima, Tsudoi Toyoda, Miki Teranishi, Kazushige Takehana, Keiko Abe, Yuji Nakai
Ingestion of collagen peptide (CP) elicits beneficial effects on the body, including improvement in blood lipid profiles, but the underlying mechanisms remain unclear. The purpose of this study was to investigate the effects of CP ingestion on the liver, which controls lipid metabolism in the body. Male BALB/cCrSlc mice were bred with the AIN-93M diet containing 14 % casein or the AIN-93M-based low-protein diet containing 10 % casein or a diet containing 6 % casein+4 % CP for 10 weeks (n 12/group). Total, free and esterified cholesterol levels in the blood decreased in the CP group...
January 16, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28089218/nkg2d-signaling-the-immune-subversive-side-of-hdac3
#5
Alicia R Folgueras, Segundo Gonzalez, Alejandro López-Soto
Natural killer (NK) cells are alerted to infected and transformed cells by local upregulation of ligands for the NK-activating receptor NKG2D. In a recent report, Greene et al. unveil a new mechanism that induces the expression of the NKG2D ligand retinoic acid early-inducible (RAE-1) in response to murine cytomegalovirus (MCMV) infection through inhibition of casein kinase 2 (CK2), an activator of the repressor histone deacetylase HDAC3.
January 11, 2017: Trends in Immunology
https://www.readbyqxmd.com/read/28088654/transcriptional-response-of-skeletal-muscle-to-a-low-protein-perinatal-diet-in-rat-offspring-at-different-ages-the-role-of-key-enzymes-of-glucose-fatty-acid-oxidation
#6
José Luiz de Brito Alves, Ana Elisa Toscano, João Henrique da Costa-Silva, Hubert Vidal, Carol Góis Leandro, Luciano Pirola
Skeletal muscle is a plastic tissue during development with distinctive acute and chronic response to maternal protein restriction. This study evaluated gene and protein expression of key-enzymes of glycolytic pathway (HK2, PFK, PDK4 and CS), and fatty acid oxidation (CPT1 and β-HAD) of two different types of skeletal muscle [soleus and extensor digitorium longus (EDL)] from offspring rats at 30 and 90 days of age, exposed to maternal isoenergetic low protein diet throughout gestation and lactation. Pups from dams fed 17% protein diet (n=5, normal protein, Np), and low protein pups from dams fed 8% casein diet (low protein, Lp, n=5) were evaluated...
December 15, 2016: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28088425/short-communication-staphylococcus-aureus-infection-modulates-expression-of-drug-transporters-and-inflammatory-biomarkers-in-mouse-mammary-gland
#7
A Oskarsson, Y Yagdiran, S Nazemi, J Tallkvist, C H Knight
Mastitis is the most common disease in dairy herds worldwide and is often caused by Staphylococcus aureus. Little is known about the effect of mastitis on transporters in the mammary gland and the effect on transporter-mediated secretion of drugs into milk. We studied gene expressions of ATP-binding cassette and solute carrier transporters in S. aureus-infected mammary glands of mice. On d 7 of lactation, NMRI mice were inoculated with 1,000 cfu of S. aureus in 2 mammary glands and with a saline vehicle in 2 control glands...
January 11, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28088252/quantification-of-whey-protein-content-in-infant-formulas-by-sds-cge
#8
Ping Feng, Christophe Fuerer, Adrienne McMahon
Protein separation by SDS-CGE (Sodium Dodecyl Sulfate - Capillary Gel Electrophoresis) followed by UV absorption at 220nm allows quantification of major proteins in raw milk. In processed dairy samples such as skim milk powder and infant formulas, signals from individual proteins are less resolved, but caseins still migrate as one family between two groups of whey proteins. In the first group, α-Lactalbumin and β-Lactoglobulin migrate as two distinct peaks. Lactosylated adducts show delayed migration times and interfere with peak separation, but both native and modified forms, as well as other low molecular weight whey proteins, still elute before the caseins...
November 18, 2016: Journal of AOAC International
https://www.readbyqxmd.com/read/28079397/mixed-compared-with-single-source-proteins-in-high-protein-diets-affect-kidney-structure-and-function-differentially-in-obese-fa-fa-zucker-rats
#9
Jessay G Devassy, Jennifer L Wojcik, Naser H M Ibrahim, Peter Zahradka, Carla G Taylor, Harold M Aukema
Questions remain regarding the potential negative effects of dietary high protein (HP) on kidney health, particularly in the context of obesity in which the risk for renal disease is already increased. To examine whether some of the variability in HP effects on kidney health may be due to source of protein, obese fa/fa Zucker rats were given HP (35% of energy from protein) diets containing either casein, soy protein, or a mixed source of animal and plant proteins for 12 weeks. Control lean and obese rats were given diets containing casein at normal protein (15% of energy from protein) levels...
October 6, 2016: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28079298/from-phosphoproteins-to-phosphoproteomes-a-historical-account
#10
REVIEW
Andrea Venerando, Luca Cesaro, Lorenzo A Pinna
The first phosphoprotein (casein) was discovered in 1883, yet the enzyme responsible for its phosphorylation was identified only 130 years later, in 2012. During the very long time elapsed between these two events, with special reference to the last decades of the 1900s, it became evident that, far from being an oddity, phosphorylation affects the majority of eukaryotic proteins during their lifespan, and that this reaction is catalysed by the members of a large family of protein kinases, susceptible to a variety of stimuli controlling nearly every aspects of life and death...
January 12, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28078412/simultaneous-determination-of-four-food-allergens-using-compact-disc-immunoassaying-technology
#11
Ahmed Ali Badran, Sergi Morais, Ángel Maquieira
A multiplex competitive microimmunoassay for the simultaneous determination of gliadin, casein, β-lactoglobulin, and ovalbumin is presented. The assay in microarray format is performed on a DVD where the allergens are physisorbed on the polycarbonate surface of the disc. The immunointeraction is detected using a mixture of specific gold-labeled antibodies and the signal amplified with the silver enhancement method. The optical density of the precipitate, read by a DVD drive, is related to the concentration of the four allergens in sample...
January 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28075359/development-of-pharmacophore-model-for-indeno-1-2-b-indoles-as-human-protein-kinase-ck2-inhibitors-and-database-mining
#12
Samer Haidar, Zouhair Bouaziz, Christelle Marminon, Tuomo Laitinen, Antti Poso, Marc Le Borgne, Joachim Jose
Protein kinase CK2, initially designated as casein kinase 2, is an ubiquitously expressed serine/threonine kinase. This enzyme, implicated in many cellular processes, is highly expressed and active in many tumor cells. A large number of compounds has been developed as inhibitors comprising different backbones. Beside others, structures with an indeno[1,2-b]indole scaffold turned out to be potent new leads. With the aim of developing new inhibitors of human protein kinase CK2, we report here on the generation of common feature pharmacophore model to further explain the binding requirements for human CK2 inhibitors...
January 9, 2017: Pharmaceuticals
https://www.readbyqxmd.com/read/28073009/how-meiosis-creates-the-single-copy-genome
#13
Mary Herbert, Attila Toth
Genome haploidization involves two meiotic divisions following a single round of DNA replication. In this issue of Developmental Cell, Argüello-Miranda et al. (2017) show that production and packaging of the single-copy genome into gametes during the second meiotic division is coordinated by a conserved casein kinase 1.
January 9, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28071015/the-changes-of-proteins-fractions-shares-in-milk-and-fermented-milk-drinks
#14
Genowefa Bonczar, Maria Walczycka, Iwona Duda
BACKGROUND: The aim of this research was to observe the changes which take place in the electrophoretic picture of milk proteins after pasteurisation and inoculation with different starter cultures (both traditional and probiotic). After incubation, the yoghurt, kefir, acidified milk, fermented Bifidobacterium bifidum drink and Lactobacillus acidophillus drink were chilled for 14 days to observe the changes which occurred. METHODS: The research materials were raw and pasteurised milk, as well as fermented milk- based drinks...
October 2016: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28069757/correction-breakpoint-cluster-region-mediated-inflammation-is-dependent-on-casein-kinase-ii
#15
Jie Meng, Jing-Jing Jiang, Toru Atsumi, Hidenori Bando, Yuko Okuyama, Lavannya Sabharwal, Ikuma Nakagawa, Haruka Higuchi, Mitsutoshi Ota, Momoko Okawara, Ryuichiro Ishitani, Osamu Nureki, Daisuke Higo, Yasunobu Arima, Hideki Ogura, Daisuke Kamimura, Masaaki Murakami
No abstract text is available yet for this article.
January 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28069123/pepsin-diffusion-in-dairy-gels-depends-on-casein-concentration-and-microstructure
#16
J Thévenot, C Cauty, D Legland, D Dupont, J Floury
Fundamental knowledge of gastric digestion had only focused on acid diffusion from the gastric fluid, but no data are available for pepsin diffusion. Using fluorescence recovery after photobleaching technique, diffusion coefficients D of fluorescein isothiocyanate (FITC)-pepsin were measured in rennet gels across a range of casein concentrations allowing to form networks of protein aggregates with different structures. To investigate the microstructural parameters of native gels, electron microscopy image analysis were performed and qualitatively related to diffusion behavior of FITC-pepsin in these dairy gels...
May 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28068403/normalisation-against-circadian-and-age-related-disturbances-enables-robust-detection-of-gene-expression-changes-in-liver-of-aged-mice
#17
Sara S Fonseca Costa, Daniel Wegmann, Jürgen A Ripperger
The expression of some genes is affected by age. To detect such age-related changes, their expression levels are related to constant marker genes. However, transcriptional noise increasing with advancing age renders difficult the identification of real age-related changes because it may affect the marker genes as well. Here, we report a selection procedure for genes appropriate to normalise the mouse liver transcriptome under various conditions including age. These genes were chosen from an initial set of 16 candidate genes defined based on a RNA-sequencing experiment and published literature...
2017: PloS One
https://www.readbyqxmd.com/read/28066137/betulin-suppressed-interleukin-1%C3%AE-induced-gene-expression-secretion-and-proteolytic-activity-of-matrix-metalloproteinase-in-cultured-articular-chondrocytes-and-production-of-matrix-metalloproteinase-in-the-knee-joint-of-rat
#18
Ho Jong Ra, Hyun Jae Lee, Ho Seung Jo, Dae Cheol Nam, Young Bok Lee, Byeong Hun Kang, Dong Kyu Moon, Dong Hee Kim, Choong Jae Lee, Sun-Chul Hwang
We investigated whether betulin affects the gene expression, secretion and proteolytic activity of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as in vivo production of MMP-3 in the rat knee joint to evaluate the potential chondroprotective effect of betulin. Rabbit articular chondrocytes were cultured and reverse transcription-polymerase chain reaction (RT-PCR) was used to measure interleukin-1β (IL-1β)-induced gene expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), ADAMTS-5 and type II collagen...
January 2017: Korean Journal of Physiology & Pharmacology
https://www.readbyqxmd.com/read/28065752/covalent-immobilization-of-trypsin-onto-modified-magnetite-nanoparticles-and-its-application-for-casein-digestion
#19
Keziban Atacan, Bekir Çakıroğlu, Mahmut Özacar
The immobilization method consists of the production magnetite nanoparticles (Fe3O4) by solvothermal treatment of FeCl3 and sodium acetate (NaAc) in the presence of ethylene glycol. Subsequently, the surface of magnetite nanoparticles was modified with a well-known polyphenol tannic acid. Trypsin was covalently immobilized on the tannic acid modified magnetite nanoparticles after exposing the modified nanoparticles to pH 9.4. Then, tryptic digestion of casein by free and immobilized trypsin was carried out for 13h and 1h, respectively...
January 5, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28065272/activity-assays-for-rhomboid-proteases
#20
E Arutyunova, K Strisovsky, M J Lemieux
Rhomboids are ubiquitous intramembrane serine proteases that are involved in various signaling pathways. This fascinating class of proteases harbors an active site buried within the lipid milieu. High-resolution structures of the Escherichia coli rhomboid GlpG with various inhibitors revealed the catalytic mechanism for rhomboid-mediated proteolysis; however, a quantitative characterization was lacking. Assessing an enzyme's catalytic parameters is important for understanding the details of its proteolytic reaction and regulatory mechanisms...
2017: Methods in Enzymology
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