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D-amino acid oxidase

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https://www.readbyqxmd.com/read/28285246/the-daoa-gene-is-associated-with-schizophrenia-in-the-taiwanese-population
#1
Che-Sheng Chu, Philip Chik-Keung Chow, Sarah Cohen-Woods, Darya Gaysina, Kwong-Yui Tang, Peter McGuffin
The gene D-amino acid oxidase activator (DAOA), which has a former name of G72, and the D-amino acid oxidase (DAO) gene have been suggested as candidate genes of schizophrenia. However, association studies have so far yielded equivocal results. We analyzed one single nucleotide polymorphism (SNP) for DAO (rs3741775) and seven SNPs for G72 (rs3916956, rs2391191, rs9558562, rs947267, rs778292, rs3918342, and rs1421292) in this study enrolling 248 schizophrenia cases and 267 controls in the Taiwanese samples. In SNP-based single locus association analyses, the rs778292 in the DAOA gene showed significant association with schizophrenia...
March 7, 2017: Psychiatry Research
https://www.readbyqxmd.com/read/28284308/enzymatic-biosensor-based-on-entrapment-of-d-amino-acid-oxidase-on-gold-nanofilm-mwcnts-nanocomposite-modified-glassy-carbon-electrode-by-sol-gel-network-analytical-applications-for-d-alanine-in-human-serum
#2
Yalda Shoja, Amir Abbas Rafati, Javad Ghodsi
Sensing and determination of d-alanine is studied by using an enzymatic biosensor which was constructed on the basis of d-amino acid oxidase (DAAO) immobilization by sol-gel film onto glassy carbon electrode surface modified with nanocomposite of gold nanofilm (Au-NF) and multiwalled carbon nanotubes (MWCNTs). The Au-NF/MWCNT nanocomposite was prepared by applying the potentiostatic technique for electrodeposition of Au-NF on the MWCNT immobilized on glassy carbon electrode surface. The modified electrode is investigated by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), linear sweep voltammetry (LSV) and cyclic voltammetry(CV) techniques...
May 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28274409/unraveling-the-inhibition-mechanism-of-cyanidin-3-sophoroside-on-polyphenol-oxidase-and-its-effect-on-enzymatic-browning-of-apples
#3
Hridya Hemachandran, Amrita Anantharaman, Sankari Mohan, Gopalakrishnan Mohan, D Thirumal Kumar, Diksha Dey, Drishty Kumar, Priyanka Dey, Amrita Choudhury, C George Priya Doss, Siva Ramamoorthy
The hunt for anti-browning agents in the food and agricultural industries aims to minimize nutritional loss and prolong post harvest storage. In the present study, the effect of cyanidin-3-sophoroside (CS) from Garcinia mangostana rind, on polyphenol oxidase (PPO) activity was investigated. The non-competitive inhibition mode of CS was determined by Lineweaver Burk plot. CS forms a ground-state complex by quenching the intrinsic fluorescence of PPO. The static quenching was temperature-dependent with an activation energy of 4...
July 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28236852/the-promising-anticancer-drug-3-bromopyruvate-is-metabolized-through-glutathione-conjugation-which-affects-chemoresistance-and-clinical-practice-an-evidence-based-view
#4
Salah Mohamed El Sayed, Hussam Baghdadi, Mohammed Zolaly, Hamdi H Almaramhy, Mongi Ayat, Jagadish G Donki
3-Bromopyruvate (3BP) is a promising effective anticancer drug against many different tumors in children and adults. 3BP exhibited strong anticancer effects in both preclinical and human studies e.g. energy depletion, oxidative stress, anti-angiogenesis, anti-metastatic effects, targeting cancer stem cells and antagonizing the Warburg effect. There is no report about 3BP metabolism to guide researchers and oncologists to improve clinical practice and prevent drug resistance. In this article, we provide evidences that 3BP is metabolized through glutathione (GSH) conjugation as a novel report where 3BP was confirmed to be attached to GSH followed by permanent loss of pharmacological effects in a picture similar to cisplatin...
March 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28220045/paternal-cocaine-taking-elicits-epigenetic-remodeling-and-memory-deficits-in-male-progeny
#5
M E Wimmer, L A Briand, B Fant, L A Guercio, A C Arreola, H D Schmidt, S Sidoli, Y Han, B A Garcia, R C Pierce
Paternal environmental perturbations including exposure to drugs of abuse can produce profound effects on the physiology and behavior of offspring via epigenetic modifications. Here we show that adult drug-naive male offspring of cocaine-exposed sires have memory formation deficits and associated reductions in NMDA receptor-mediated hippocampal synaptic plasticity. Reduced levels of the endogenous NMDA receptor co-agonist d-serine were accompanied by increased expression of the d-serine degrading enzyme d-amino acid oxidase (Dao1) in the hippocampus of cocaine-sired male progeny...
February 21, 2017: Molecular Psychiatry
https://www.readbyqxmd.com/read/28202572/differential-regulation-of-nmda-receptors-by-d-serine-and-glycine-in-mammalian-spinal-locomotor-networks
#6
David Acton, Gareth Brian Miles
Activation of N-methyl-D-aspartate receptors (NMDARs) requires the binding of a co-agonist, either D-serine or glycine, in addition to glutamate. Changes in occupancy of the co-agonist binding site are proposed to modulate neural networks including those controlling swimming in frog tadpoles. Here, we characterize regulation of the NMDAR co-agonist binding site in mammalian spinal locomotor networks. Blockade of NMDARs by D(-)-2-amino-5-phosphonopentanoic acid (D-APV) or 5,7-dichlorokynurenic acid reduced the frequency and amplitude of pharmacologically induced locomotor-related activity recorded from the ventral roots of spinal-cord preparations from neonatal mice...
February 15, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28195456/diversity-oriented-synthesis-of-coumarin-linked-benzimidazoles-via-a-one-pot-three-step-intramolecular-knoevenagel-cyclization
#7
Po-Hsin Eric Yao, Sunil Kumar, Yu-Li Liu, Chiu-Ping Eikon Fang, Chia-Chen Liu, Chung-Ming Sun
Diversity-oriented synthesis of coumarin-linked benzimidazoles from N-(2-aminophenyl)-2-cyanoacetamide was achieved via a one-pot, three-step sequential reaction in excellent yields. In situ intramolecular cyclization of the cyanoacetamide afforded benzimidazoles which subsequently underwent a Knoevenagel condensation of the 2-cyanomethylbenzimidazoles with salicylaldehydes promoted by triethylamine to reach the target compounds. An important intermediate, 2-(2-imino-2H-chromen-3-yl)-1H-benzimidazole was characterized by X-ray analysis and further hydrolyzed to 2-(coumarin-3-yl)benzimidazole in acidic condition...
February 14, 2017: ACS Combinatorial Science
https://www.readbyqxmd.com/read/28191958/specific-ligation-of-two-multimeric-enzymes-with-native-peptides-and-immobilization-with-controlled-molar-ratio
#8
Kun Du, Jinjin Zhao, Jian Sun, Wei Feng
d-Amino acid oxidases (DAAOs) are flavor enzymes and have been used in resolution of racemic amino acids and manufacturing of pharmaceuticals. However, the evolved H2O2 during the catalysis has deleterious and inhibitory effects. Decomposition of the hydrogen peroxide by catalase (CAT) can eliminate the negative effects. DAAO and CAT are dimeric and tetrameric proteins, respectively. Here, the N-terminus of the DAAO subunits has been specifically ligated to the C-terminus of the CAT subunits with native peptides through intein-mediated in vivo protein splicing...
February 24, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28159840/the-role-of-a-fad-cofactor-in-the-regulation-of-acetohydroxyacid-synthase-by-redox-signaling-molecules
#9
Thierry Lonhienne, Mario D Garcia, Luke W Guddat
Acetohydroxyacid synthase (AHAS) catalyzes the first step of branched-chain amino acid (BCAA) biosynthesis, a pathway essential to the lifecycle of plants and microorganisms. This enzyme is of high interest because its inhibition is at the base of the exceptional potency of herbicides and potentially a target for the discovery of new antimicrobial drugs. The enzyme has conserved attributes from its predicted ancestor, pyruvate oxidase, such as a ubiquinone-binding site and the requirement for FAD as cofactor...
February 3, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28159615/immobilization-of-r-%C3%AF-transaminase-on-mno2-nanorods-for-catalyzing-the-conversion-of-r-1-phenylethylamine
#10
Jian Sun, Wen-Hui Cui, Kun Du, Qian Gao, Mengmeng Du, Peijun Ji, Wei Feng
R-ɷ-transaminases transfer an amino group from an amino donor (e.g. (R)-1-phenylethylamine) onto an amino acceptor (e.g. pyruvate), resulting a co-product (e.g. d-alanine). This work intends to immobilize R-ɷ-Transaminase on MnO2 nanorods to achieve multienzyme catalysis. R-ɷ-Transaminase (RTA) and d-amino acid oxidase (DAAO) have been fused to an elastin-like polypeptide (ELP) separately through genetic engineering of the enzymes. ELP-RTA and ELP-DAAO have been separately immobilized on polydopamine-coated MnO2 nanorods...
March 10, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28153250/fabrication-of-enzyme-reactor-utilizing-magnetic-porous-polymer-membrane-for-screening-d-amino-acid-oxidase-inhibitors
#11
Jun Fang Jiang, Juan Qiao, Xiao Yu Mu, Myeong Hee Moon, Li Qi
In this work, a unique D-amino acid oxidase reactor for enhanced enzymolysis efficiency is presented. A kind of magnetic polymer matrices, composed of iron oxide nanoparticles and porous polymer membrane (poly styrene-co-maleic anhydride), was prepared. With covalent bonding D-Amino acid oxidase on the surface of the matrices and characterization of scanning electron microscope and vibrating sample magnetometer, it demonstrated that the membrane enzyme reactor was successfully constructed. The enzymolysis efficiency of the enzyme reactor was evaluated and the apparent Michaelis-Menten constants of D-Amino acid oxidase were determined (Km was 1...
April 1, 2017: Talanta
https://www.readbyqxmd.com/read/28149023/status-of-serum-calcium-vitamin-d-and-parathyroid-hormone-and-hematological-indices-among-lead-exposed-jewelry-workers-in-dhaka-bangladesh
#12
I Mazumdar, K Goswami, Md Suhrab Ali
Jewelry utilizes lead either directly or as a base metal. Costume jewelry requires lead before molding and plating the product with valuable metals. Therefore, such ornaments have a great potential to release heavy metals having health hazards. Also, jewelry makers engaged in preparing German silver, an alloy, apply lead in smelting, alloying, rolling and milling silver wires and pieces. The metal is taken up by blood, soft tissues and bone. The biological effects of lead are dependent upon the level and duration of exposure...
March 2017: Indian Journal of Clinical Biochemistry: IJCB
https://www.readbyqxmd.com/read/28137610/evidence-that-d-cysteine-protects-mice-from-gastric-damage-via-hydrogen-sulfide-produced-by-d-amino-acid-oxidase
#13
Luan Kelves M Souza, Thiago S L Araújo, Nayara A Sousa, Francisca Beatriz M Sousa, Kerolayne M Nogueira, Lucas A D Nicolau, Jand Venes R Medeiros
Hydrogen sulfide (H2S) is a signaling molecule in the gastrointestinal tract. H2S production can derive from d-cysteine via various pathways, thus pointing to a new therapeutic approach: delivery of H2S to specific tissues. This study was designed to evaluate the concentration and effects of H2S (generated by d-amino acid oxidase [DAO] from d-cysteine) in the gastric mucosa and the protective effects against ethanol-induced lesions in mice. Mice were treated with l-cysteine or d-cysteine (100 mg/kg per os)...
April 1, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28053093/catalytic-determinants-of-alkene-production-by-the-cytochrome-p450-peroxygenase-oletje
#14
Sarah Matthews, James D Belcher, Kang Lan Tee, Hazel M Girvan, Kirsty J McLean, Stephen Ej Rigby, Colin W Levy, David Leys, David A Parker, Richard T Blankley, Andrew W Munro
The Jeotgalicoccus sp. peroxygenase cytochrome P450 OleTJE (CYP152L1) is a hydrogen peroxide-driven oxidase that catalyzes oxidative decarboxylation of fatty acids, producing terminal alkenes with applications as fine chemicals and biofuels. Understanding mechanisms that favor decarboxylation over fatty acid hydroxylation in OleTJE could enable protein engineering to improve catalysis or to introduce decarboxylation activity into P450s with different substrate preferences. In this manuscript, we have focused on OleTJE active site residues Phe79, His85 and Arg245 to interrogate their roles in substrate binding and catalytic activity...
January 4, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28025800/intrathecal-delivery-of-ssaav9-dao-extends-survival-in-sod1-g93a-als-mice
#15
Wan Wang, Weisong Duan, Ying Wang, Di Wen, Yakun Liu, Zhongyao Li, Haojie Hu, Hongying Cui, Can Cui, Huiqian Lin, Chunyan Li
Amyotrophic lateral sclerosis (ALS) is an adult-onset, irreversible neurodegenerative disease that leads to progressive paralysis and inevitable death 3-5 years after diagnosis. The mechanisms underlying this process remain unknown, but new evidence indicates that accumulating levels of D-serine result from the downregulation of D-amino acid oxidase (DAO) and that this is a novel mechanism that leads to motoneuronal death in ALS via N-methyl-D-aspartate receptor-mediated cell toxicity. Here, we explored a new therapeutic approach to ALS by overexpressing DAO in the lumbar region of the mouse spinal cord using a single stranded adeno-associated virus serotype 9 (ssAAV9) vector...
December 26, 2016: Neurochemical Research
https://www.readbyqxmd.com/read/28007379/subunit-ccoq-is-involved-in-the-assembly-of-the-cbb3-type-cytochrome-c-oxidases-from-pseudomonas-stutzeri-zobell-but-not-required-for-their-activity
#16
Martin Kohlstaedt, Sabine Buschmann, Julian D Langer, Hao Xie, Hartmut Michel
The Cbb3-type cytochrome c oxidases (Cbb3-CcOs), the second most abundant CcOs, catalyze the reduction of molecular oxygen to water, even at micromolar oxygen concentrations. In Pseudomonas stutzeri ZoBell, two tandemly organized cbb3-operons encode the isoforms Cbb3-1 and Cbb3-2 both possessing subunits CcoN, CcoO and CcoP. However, only the cbb3-2 operon contains an additional ccoQ gene. CcoQ consists of 62 amino acids and is predicted to possess one transmembrane spanning helix. The physiological role of CcoQ was investigated based on a CcoQ-deletion mutant and wild-type Cbb3-2 crystals not containing subunit CcoQ...
December 20, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28005309/an-engineered-tryptophan-synthase-opens-new-enzymatic-pathways-to-%C3%AE-methyltryptophan-and-derivatives
#17
Daniel Francis, Michael Winn, Jonathan Latham, Michael F Greaney, Jason Micklefield
β-Methyltryptophans (β-mTrp) are precursors in the biosynthesis of bioactive natural products and are used in the synthesis of peptidomimetic-based therapeutics. Currently β-mTrp is produced by inefficient multistep synthetic methods. Here we demonstrate how an engineered variant of tryptophan synthase from Salmonella (StTrpS) can catalyse the efficient condensation of l-threonine and various indoles to generate β-mTrp and derivatives in a single step. Although l-serine is the natural substrate for TrpS, targeted mutagenesis of the StTrpS active site provided a variant (βL166V) that can better accommodate l-Thr as a substrate...
December 22, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27995572/intramuscular-delivery-of-scaav9-higf1-prolongs-survival-in-the-hsod1-g93a-als-mouse-model-via-upregulation-of-d-amino-acid-oxidase
#18
HuiQian Lin, HaoJie Hu, WeiSong Duan, YaLing Liu, GuoJun Tan, ZhongYao Li, YaKun Liu, BinBin Deng, XueQin Song, Wan Wang, Di Wen, Ying Wang, ChunYan Li
Self-complementary adeno-associated viral vector 9 (scAAV9) has been confirmed to be an efficient AAV serotype for gene transfer to the central nervous system (CNS). Neurotrophic factors have been considered to be therapeutic targets for amyotrophic lateral sclerosis (ALS). In the present study, we intramuscularly injected scAAV9 encoding human insulin-like growth factor 1 (hIGF1) into an hSOD1(G93A) ALS mouse model. We observed that scAAV9-hIGF1 significantly reduced the loss of motor neurons of the anterior horn in the lumbar spinal cord and delayed muscle atrophy in ALS mice...
December 19, 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/27815320/structure-models-of-g72-the-product-of-a-susceptibility-gene-to-schizophrenia
#19
Yusuke Kato, Kiyoshi Fukui
The G72 gene is one of the most susceptible genes to schizophrenia and is contained exclusively in the genomes of primates. The product of the G72 gene modulates the activity of D-amino acid oxidase (DAO) and is a small protein prone to aggregate, which hampers its structural studies. In addition, lack of a known structure of a homologue makes it difficult to use the homology modelling method for the prediction of the structure. Thus, we first developed a hybrid ab initio approach for small proteins prior to the prediction of the structure of G72...
November 3, 2016: Journal of Biochemistry
https://www.readbyqxmd.com/read/27803669/the-role-of-hydrogen-sulfide-in-renal-system
#20
REVIEW
Xu Cao, Jin-Song Bian
Hydrogen sulfide has gained recognition as the third gaseous signaling molecule after nitric oxide and carbon monoxide. This review surveys the emerging role of H2S in mammalian renal system, with emphasis on both renal physiology and diseases. H2S is produced redundantly by four pathways in kidney, indicating the abundance of this gaseous molecule in the organ. In physiological conditions, H2S was found to regulate the excretory function of the kidney possibly by the inhibitory effect on sodium transporters on renal tubular cells...
2016: Frontiers in Pharmacology
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