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D-amino acid oxidase

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https://www.readbyqxmd.com/read/28428746/expression-of-d-amino-acid-oxidase-dao-daao-and-d-amino-acid-oxidase-activator-daoa-g72-during-development-and-aging-in-the-human-post-mortem-brain
#1
Vinita Jagannath, Zoya Marinova, Camelia-Maria Monoranu, Susanne Walitza, Edna Grünblatt
In the brain, D-amino acid oxidase (DAO/DAAO) mainly oxidizes D-serine, a co-agonist of the N-methyl-D-aspartate (NMDA) receptors. Thus, DAO can regulate the function of NMDA receptors via D-serine breakdown. Furthermore, DAO activator (DAOA)/G72 has been reported as both DAOA and repressor. The co-expression of DAO and DAOA genes and proteins in the human brain is not yet elucidated. The aim of this study was to understand the regional and age span distribution of DAO and DAOA (mRNA and protein) in a concomitant manner...
2017: Frontiers in Neuroanatomy
https://www.readbyqxmd.com/read/28419969/neurotoxic-effects-of-silver-nanoparticles-and-the-protective-role-of-rutin
#2
Mona M Ahmed, Mohamed M A Hussein
The toxicological studies on silver nanoparticles (Ag-NPs) have become a hot topic over the past few decades due to their unique properties on the nanoscale and widespread in many commercial products that launched into the market recently. This study was undertaken to shed light on Ag-NPs toxicity on neurotransmitters with special emphasis on the impact of concurrent administration of rutin with Ag-NPs in the experimental rats. The oral administration of Ag-NPs in rats induced brain oxidative stress, significant alterations in neurotransmitters and amino acids...
April 15, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28389772/anoxybacillus-sp-strain-uark-01-a-new-thermophilic-soil-bacterium-with-hyperthermostable-alkaline-laccase-activity
#3
Thamir H Al-Kahem Al-Balawi, Adam L Wood, Alexis Solis, Ted Cooper, Ravi D Barabote
We describe the isolation and characteristics of a novel thermophilic bacterium from soil. The organism is a member of the Anoxybacillus genus based on phylogenetic analysis of the 16S rRNA gene. The 16S rRNA of the organism shares >99% sequence identity with those of two species, Anoxybacillus rupiensis and A. geothermalis. We named this isolate as Anoxybacillus sp. strain UARK-01. UARK-01 grows optimally in the presence of oxygen at 55 °C and pH 8. It grew excellently in the presence of lignin as the sole carbon source...
April 8, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28377778/foliar-and-seed-application-of-amino-acids-affects-the-antioxidant-metabolism-of-the-soybean-crop
#4
Walquíria F Teixeira, Evandro B Fagan, Luís H Soares, Renan C Umburanas, Klaus Reichardt, Durval D Neto
In recent years, the application of natural substances on crops has been intensified in order to increase the resistance and yield of the soybean crop. Among these products are included plant biostimulants that may contain algae extracts, amino acids, and plant regulators in their composition. However, there is little information on the isolated effect of each of these constituents. The objective of this research was to evaluate the effect of the application of isolated amino acids on the antioxidant metabolism of the soybean crop...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28369975/the-redox-state-of-the-apoplast-influences-the-acclimation-of-photosynthesis-and-leaf-metabolism-to-changing-irradiance
#5
Barbara Karpinska, Kaiming Zhang, Brwa Rasool, Daria Pastok, Jenny Morris, Susan R Verrall, Pete E Hedley, Robert D Hancock, Christine H Foyer
The redox state of the apoplast is largely determined by ascorbate oxidase (AO) activity. The influence of AO activity on leaf acclimation to changing irradiance was explored in wild type (WT) and transgenic tobacco (Nicotiana tobaccum) lines containing either high (PAO) or low (TAO) AO activity at low (LL; 250 µmol m(-2) s(-1) ) and high (HL; 1600 µmol m(-2) s(-1) ) irradiance and following the transition from HL to LL. AO activities changed over the photoperiod, particularly in the PAO plants. AO activity had little effect on leaf ascorbate, which was significantly higher under HL than LL...
April 1, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28355481/mechanistic-studies-of-an-amine-oxidase-derived-from-d-amino-acid-oxidase
#6
Elizabeth E Trimmer, Udayanga S Wanninayake, Paul F Fitzpatrick
The flavoprotein d-amino acid oxidase has long served as a paradigm for understanding the mechanism of oxidation of amino acids by flavoproteins. Recently, a mutant d-amino acid oxidase (Y228L/R283G) that catalyzed the oxidation of amines rather than amino acids was described [Yasukawa, K., et al. (2014) Angew. Chem., Int. Ed. 53, 4428-4431]. We describe here the use of pH and kinetic isotope effects with (R)-α-methylbenzylamine as a substrate to determine whether the mutant enzyme utilizes the same catalytic mechanism as the wild-type enzyme...
April 11, 2017: Biochemistry
https://www.readbyqxmd.com/read/28285246/the-daoa-gene-is-associated-with-schizophrenia-in-the-taiwanese-population
#7
Che-Sheng Chu, Philip Chik-Keung Chow, Sarah Cohen-Woods, Darya Gaysina, Kwong-Yui Tang, Peter McGuffin
The gene D-amino acid oxidase activator (DAOA), which has a former name of G72, and the D-amino acid oxidase (DAO) gene have been suggested as candidate genes of schizophrenia. However, association studies have so far yielded equivocal results. We analyzed one single nucleotide polymorphism (SNP) for DAO (rs3741775) and seven SNPs for G72 (rs3916956, rs2391191, rs9558562, rs947267, rs778292, rs3918342, and rs1421292) in this study enrolling 248 schizophrenia cases and 267 controls in the Taiwanese samples. In SNP-based single locus association analyses, the rs778292 in the DAOA gene showed significant association with schizophrenia...
March 7, 2017: Psychiatry Research
https://www.readbyqxmd.com/read/28284308/enzymatic-biosensor-based-on-entrapment-of-d-amino-acid-oxidase-on-gold-nanofilm-mwcnts-nanocomposite-modified-glassy-carbon-electrode-by-sol-gel-network-analytical-applications-for-d-alanine-in-human-serum
#8
Yalda Shoja, Amir Abbas Rafati, Javad Ghodsi
Sensing and determination of d-alanine is studied by using an enzymatic biosensor which was constructed on the basis of d-amino acid oxidase (DAAO) immobilization by sol-gel film onto glassy carbon electrode surface modified with nanocomposite of gold nanofilm (Au-NF) and multiwalled carbon nanotubes (MWCNTs). The Au-NF/MWCNT nanocomposite was prepared by applying the potentiostatic technique for electrodeposition of Au-NF on the MWCNT immobilized on glassy carbon electrode surface. The modified electrode is investigated by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), linear sweep voltammetry (LSV) and cyclic voltammetry(CV) techniques...
May 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28274409/unraveling-the-inhibition-mechanism-of-cyanidin-3-sophoroside-on-polyphenol-oxidase-and-its-effect-on-enzymatic-browning-of-apples
#9
Hridya Hemachandran, Amrita Anantharaman, Sankari Mohan, Gopalakrishnan Mohan, D Thirumal Kumar, Diksha Dey, Drishty Kumar, Priyanka Dey, Amrita Choudhury, C George Priya Doss, Siva Ramamoorthy
The hunt for anti-browning agents in the food and agricultural industries aims to minimize nutritional loss and prolong post harvest storage. In the present study, the effect of cyanidin-3-sophoroside (CS) from Garcinia mangostana rind, on polyphenol oxidase (PPO) activity was investigated. The non-competitive inhibition mode of CS was determined by Lineweaver Burk plot. CS forms a ground-state complex by quenching the intrinsic fluorescence of PPO. The static quenching was temperature-dependent with an activation energy of 4...
July 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28236852/the-promising-anticancer-drug-3-bromopyruvate-is-metabolized-through-glutathione-conjugation-which-affects-chemoresistance-and-clinical-practice-an-evidence-based-view
#10
Salah Mohamed El Sayed, Hussam Baghdadi, Mohammed Zolaly, Hamdi H Almaramhy, Mongi Ayat, Jagadish G Donki
3-Bromopyruvate (3BP) is a promising effective anticancer drug against many different tumors in children and adults. 3BP exhibited strong anticancer effects in both preclinical and human studies e.g. energy depletion, oxidative stress, anti-angiogenesis, anti-metastatic effects, targeting cancer stem cells and antagonizing the Warburg effect. There is no report about 3BP metabolism to guide researchers and oncologists to improve clinical practice and prevent drug resistance. In this article, we provide evidences that 3BP is metabolized through glutathione (GSH) conjugation as a novel report where 3BP was confirmed to be attached to GSH followed by permanent loss of pharmacological effects in a picture similar to cisplatin...
March 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28220045/paternal-cocaine-taking-elicits-epigenetic-remodeling-and-memory-deficits-in-male-progeny
#11
M E Wimmer, L A Briand, B Fant, L A Guercio, A C Arreola, H D Schmidt, S Sidoli, Y Han, B A Garcia, R C Pierce
Paternal environmental perturbations including exposure to drugs of abuse can produce profound effects on the physiology and behavior of offspring via epigenetic modifications. Here we show that adult drug-naive male offspring of cocaine-exposed sires have memory formation deficits and associated reductions in NMDA receptor-mediated hippocampal synaptic plasticity. Reduced levels of the endogenous NMDA receptor co-agonist d-serine were accompanied by increased expression of the d-serine degrading enzyme d-amino acid oxidase (Dao1) in the hippocampus of cocaine-sired male progeny...
February 21, 2017: Molecular Psychiatry
https://www.readbyqxmd.com/read/28202572/differential-regulation-of-nmda-receptors-by-d-serine-and-glycine-in-mammalian-spinal-locomotor-networks
#12
David Acton, Gareth Brian Miles
Activation of N-methyl-D-aspartate receptors (NMDARs) requires the binding of a co-agonist, either D-serine or glycine, in addition to glutamate. Changes in occupancy of the co-agonist binding site are proposed to modulate neural networks including those controlling swimming in frog tadpoles. Here, we characterize regulation of the NMDAR co-agonist binding site in mammalian spinal locomotor networks. Blockade of NMDARs by D(-)-2-amino-5-phosphonopentanoic acid (D-APV) or 5,7-dichlorokynurenic acid reduced the frequency and amplitude of pharmacologically induced locomotor-related activity recorded from the ventral roots of spinal-cord preparations from neonatal mice...
February 15, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28195456/diversity-oriented-synthesis-of-coumarin-linked-benzimidazoles-via-a-one-pot-three-step-intramolecular-knoevenagel-cyclization
#13
Po-Hsin Eric Yao, Sunil Kumar, Yu-Li Liu, Chiu-Ping Fang, Chia-Chen Liu, Chung-Ming Sun
Diversity-oriented synthesis of coumarin-linked benzimidazoles from N-(2-aminophenyl)-2-cyanoacetamide was achieved via a one-pot, three-step sequential reaction in excellent yields. In situ intramolecular cyclization of the cyanoacetamide afforded benzimidazoles which subsequently underwent a Knoevenagel condensation of the 2-cyanomethylbenzimidazoles with salicylaldehydes promoted by triethylamine to reach the target compounds. An important intermediate, 2-(2-imino-2H-chromen-3-yl)-1H-benzimidazole was characterized by X-ray analysis and further hydrolyzed to 2-(coumarin-3-yl)benzimidazole in acidic condition...
February 22, 2017: ACS Combinatorial Science
https://www.readbyqxmd.com/read/28191958/specific-ligation-of-two-multimeric-enzymes-with-native-peptides-and-immobilization-with-controlled-molar-ratio
#14
Kun Du, Jinjin Zhao, Jian Sun, Wei Feng
d-Amino acid oxidases (DAAOs) are flavor enzymes and have been used in resolution of racemic amino acids and manufacturing of pharmaceuticals. However, the evolved H2O2 during the catalysis has deleterious and inhibitory effects. Decomposition of the hydrogen peroxide by catalase (CAT) can eliminate the negative effects. DAAO and CAT are dimeric and tetrameric proteins, respectively. Here, the N-terminus of the DAAO subunits has been specifically ligated to the C-terminus of the CAT subunits with native peptides through intein-mediated in vivo protein splicing...
February 24, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28159840/the-role-of-a-fad-cofactor-in-the-regulation-of-acetohydroxyacid-synthase-by-redox-signaling-molecules
#15
Thierry Lonhienne, Mario D Garcia, Luke W Guddat
Acetohydroxyacid synthase (AHAS) catalyzes the first step of branched-chain amino acid (BCAA) biosynthesis, a pathway essential to the lifecycle of plants and microorganisms. This enzyme is of high interest because its inhibition is at the base of the exceptional potency of herbicides and potentially a target for the discovery of new antimicrobial drugs. The enzyme has conserved attributes from its predicted ancestor, pyruvate oxidase, such as a ubiquinone-binding site and the requirement for FAD as cofactor...
March 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28159615/immobilization-of-r-%C3%AF-transaminase-on-mno2-nanorods-for-catalyzing-the-conversion-of-r-1-phenylethylamine
#16
Jian Sun, Wen-Hui Cui, Kun Du, Qian Gao, Mengmeng Du, Peijun Ji, Wei Feng
R-ɷ-transaminases transfer an amino group from an amino donor (e.g. (R)-1-phenylethylamine) onto an amino acceptor (e.g. pyruvate), resulting a co-product (e.g. d-alanine). This work intends to immobilize R-ɷ-Transaminase on MnO2 nanorods to achieve multienzyme catalysis. R-ɷ-Transaminase (RTA) and d-amino acid oxidase (DAAO) have been fused to an elastin-like polypeptide (ELP) separately through genetic engineering of the enzymes. ELP-RTA and ELP-DAAO have been separately immobilized on polydopamine-coated MnO2 nanorods...
March 10, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28153250/fabrication-of-enzyme-reactor-utilizing-magnetic-porous-polymer-membrane-for-screening-d-amino-acid-oxidase-inhibitors
#17
Jun Fang Jiang, Juan Qiao, Xiao Yu Mu, Myeong Hee Moon, Li Qi
In this work, a unique D-amino acid oxidase reactor for enhanced enzymolysis efficiency is presented. A kind of magnetic polymer matrices, composed of iron oxide nanoparticles and porous polymer membrane (poly styrene-co-maleic anhydride), was prepared. With covalent bonding D-Amino acid oxidase on the surface of the matrices and characterization of scanning electron microscope and vibrating sample magnetometer, it demonstrated that the membrane enzyme reactor was successfully constructed. The enzymolysis efficiency of the enzyme reactor was evaluated and the apparent Michaelis-Menten constants of D-Amino acid oxidase were determined (Km was 1...
April 1, 2017: Talanta
https://www.readbyqxmd.com/read/28149023/status-of-serum-calcium-vitamin-d-and-parathyroid-hormone-and-hematological-indices-among-lead-exposed-jewelry-workers-in-dhaka-bangladesh
#18
I Mazumdar, K Goswami, Md Suhrab Ali
Jewelry utilizes lead either directly or as a base metal. Costume jewelry requires lead before molding and plating the product with valuable metals. Therefore, such ornaments have a great potential to release heavy metals having health hazards. Also, jewelry makers engaged in preparing German silver, an alloy, apply lead in smelting, alloying, rolling and milling silver wires and pieces. The metal is taken up by blood, soft tissues and bone. The biological effects of lead are dependent upon the level and duration of exposure...
March 2017: Indian Journal of Clinical Biochemistry: IJCB
https://www.readbyqxmd.com/read/28137610/evidence-that-d-cysteine-protects-mice-from-gastric-damage-via-hydrogen-sulfide-produced-by-d-amino-acid-oxidase
#19
Luan Kelves M Souza, Thiago S L Araújo, Nayara A Sousa, Francisca Beatriz M Sousa, Kerolayne M Nogueira, Lucas A D Nicolau, Jand Venes R Medeiros
Hydrogen sulfide (H2S) is a signaling molecule in the gastrointestinal tract. H2S production can derive from d-cysteine via various pathways, thus pointing to a new therapeutic approach: delivery of H2S to specific tissues. This study was designed to evaluate the concentration and effects of H2S (generated by d-amino acid oxidase [DAO] from d-cysteine) in the gastric mucosa and the protective effects against ethanol-induced lesions in mice. Mice were treated with l-cysteine or d-cysteine (100 mg/kg per os)...
April 1, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28053093/catalytic-determinants-of-alkene-production-by-the-cytochrome-p450-peroxygenase-oletje
#20
Sarah Matthews, James D Belcher, Kang Lan Tee, Hazel M Girvan, Kirsty J McLean, Stephen E J Rigby, Colin W Levy, David Leys, David A Parker, Richard T Blankley, Andrew W Munro
The Jeotgalicoccus sp. peroxygenase cytochrome P450 OleTJE (CYP152L1) is a hydrogen peroxide-driven oxidase that catalyzes oxidative decarboxylation of fatty acids, producing terminal alkenes with applications as fine chemicals and biofuels. Understanding mechanisms that favor decarboxylation over fatty acid hydroxylation in OleTJE could enable protein engineering to improve catalysis or to introduce decarboxylation activity into P450s with different substrate preferences. In this manuscript, we have focused on OleTJE active site residues Phe(79), His(85), and Arg(245) to interrogate their roles in substrate binding and catalytic activity...
March 24, 2017: Journal of Biological Chemistry
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