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https://www.readbyqxmd.com/read/29166670/age-related-differences-in-the-respiratory-microbiota-of-chickens
#1
Laura Glendinning, Gerry McLachlan, Lonneke Vervelde
In this era of next generation sequencing technologies it is now possible to characterise the chicken respiratory microbiota without the biases inherent to traditional culturing techniques. However, little research has been performed in this area. In this study we characterise and compare buccal, nasal and lung microbiota samples from chickens in three different age groups using 16S rRNA gene analysis. Buccal and nasal swabs were taken from birds aged 2 days (n = 5), 3 weeks (n = 5) and 30 months (n = 6). Bronchoalveolar lavage (BAL) samples were also collected alongside reagent only controls...
2017: PloS One
https://www.readbyqxmd.com/read/29166547/chemical-metabolome-assay-by-high-resolution-orbitrap-mass-spectrometry-and-assessment-of-associated-antitumoral-activity-of-actinocephalus-divaricatus
#2
Ana C Zanatta, Angela Mari, Milena Masullo, Iracilda Zeppone Carlos, Wagner Vilegas, Sonia Piacente, Lourdes Campaner Dos Santos
RATIONALE: Actinocephalus divaricatus (Eriocaulaceae) is an important source of income for the rural communities as it is sold as an ornamental plant. To date, no investigation has been conducted concerning the chemical composition and biological studies of the aerial parts of A. divaricatus. METHODS: The methanolic extract of the aerial parts of this species was chemically characterized. We applied an analytical dereplication approach based on Liquid Chromatography coupled to High Resolution Orbitrap Mass Spectrometry in order to develop, identify and define rapidly the metabolite fingerprint of the aerial parts of A...
November 22, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29164067/tick-borne-transmission-of-murine-gammaherpesvirus-68
#3
Valeria Hajnická, Marcela Kúdelová, Iveta Štibrániová, Mirko Slovák, Pavlína Bartíková, Zuzana Halásová, Peter Pančík, Petra Belvončíková, Michaela Vrbová, Viera Holíková, Rosemary S Hails, Patricia A Nuttall
Herpesviruses are a large group of DNA viruses infecting mainly vertebrates. Murine gammaherpesvirus 68 (MHV68) is often used as a model in studies of the pathogenesis of clinically important human gammaherpesviruses such as Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus. This rodent virus appears to be geographically widespread; however, its natural transmission cycle is unknown. Following detection of MHV68 in field-collected ticks, including isolation of the virus from tick salivary glands and ovaries, we investigated whether MHV68 is a tick-borne virus...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29163803/hat1-induces-lung-cancer-cell-apoptosis-via-up-regulating-fas
#4
Na Han, Lei Shi, Qiuyun Guo, Wei Sun, Yang Yu, Li Yang, Xiaoxi Zhang, Mengxian Zhang
The dysfunction of apoptosis is one of the factors contributing to lung cancer (LC) growth. Histone acetyltransferase HAT1 can up regulate cell apoptosis. This study aims to investigate the mechanism by which HAT1 induces LC cell (LCC) apoptosis via up regulating the expression of Fas. In this study, the surgically removed human LC tissues were collected. LCCs were isolated from the LC tissues and analyzed for the expression of HAT1 and Fas by RT-qPCR and Western blotting. We observed that the expression of Fas was negatively correlated with PAR2 in LCCs...
October 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/29163647/comparison-of-diagnostic-tests-for-the-detection-of-bovine-brucellosis-in-the-natural-cases-of-abortion
#5
V Mahajan, H S Banga, G Filia, M P Gupta, K Gupta
Rapid and precise diagnosis in natural field cases of bovine abortion caused by Brucella abortus warrants the use of the most sensitive and reliable diagnostic methods. In the present study, bacterial isolation, serology, gross, histopathology, immunohistochemistry and polymerase chain reaction technique(s) were applied. Sero-prevalence studies showed the rate of 28.86% positive cases using the competitive ELISA. Histopathological changes were mainly seen in the placenta, fetal lungs, kidney, liver and spleen...
2017: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/29162539/modulation-of-diverse-oncogenic-transcription-factors-by-thymoquinone-an-essential-oil-compound-isolated-from-the-seeds-of-nigella-sativa-linn
#6
REVIEW
Muthu K Shanmugam, Frank Arfuso, Alan Prem Kumar, Lingzhi Wang, Boon Cher Goh, Kwang Seok Ahn, Anupam Bishayee, Gautam Sethi
Thymoquinone (TQ), isolated almost fifty years ago, is the main bioactive constituent of black seed essential oil extracted from the seed of Nigella sativa. TQ has been shown to have promising effects against a variety of inflammatory diseases and cancer. Cancer development is a multistep process where normal cells acquire qualities that enable the cells to proliferate continuously and migrate to distant sites in the human body. Drugs that interfere with this process are considered potential anti-cancer therapeutics, which may ultimately result in their clinical usage...
November 18, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29162436/effect-of-intermedin-adrenomedullin2-on-the-pulmonary-vascular-bed-in-hypoxia-induced-pulmonary-hypertensive-rats
#7
Gokcen Telli, Banu Cahide Tel, Nilgun Yersal, Petek Korkusuz, Bulent Gumusel
AIMS: This study aimed to investigate the effect and mechanism of action of intermedin/adrenomedullin2 (IMD/AM2) on the pulmonary vascular bed in pulmonary hypertensive rats. MATERIALS AND METHODS: Male Sprague-Dawley rats were exposed to hypobaric hypoxia for 3 weeks to induce pulmonary hypertension (PHT). The development of PHT was confirmed by histopathological analyses and measurement of hematocrit, basal perfusion pressure, and right ventricle hypertrophy. Subsequently, the effect of IMD/AM2 in pulmonary hypertensive rats was assessed with both, isolated organ bath and isolated lung perfusion studies...
November 18, 2017: Life Sciences
https://www.readbyqxmd.com/read/29162154/pigeon-paramyxovirus-type-1-from-a-fatal-human-case-induces-pneumonia-in-experimentally-infected-cynomolgus-macaques-macaca-fascicularis
#8
Thijs Kuiken, Pascal Buijs, Peter van Run, Geert van Amerongen, Marion Koopmans, Bernadette van den Hoogen
Although avian paramyxovirus type 1 is known to cause mild transient conjunctivitis in human beings, there are two recent reports of fatal respiratory disease in immunocompromised human patients infected with the pigeon lineage of the virus (PPMV-1). In order to evaluate the potential of PPMV-1 to cause respiratory tract disease, we inoculated a PPMV-1 isolate (hPPMV-1/Netherlands/579/2003) from an immunocompromised human patient into three healthy cynomolgus macaques (Macaca fascicularis) and examined them by clinical, virological, and pathological assays...
November 21, 2017: Veterinary Research
https://www.readbyqxmd.com/read/29162036/sorafenib-treatment-for-papillary-thyroid-carcinoma-with-diffuse-lung-metastases-in-a-child-with-autism-spectrum-disorder-a-case-report
#9
Yousuke Higuchi, Takayuki Motoki, Hisashi Ishida, Kiichiro Kanamitsu, Kana Washio, Takanori Oyama, Takuo Noda, Yasuko Tsurumaru, Ayumi Okada, Hirokazu Tsukahara, Akira Shimada
BACKGROUND: Pediatric papillary thyroid carcinoma frequently presents with lymph node involvement and distant metastases. Sorafenib, an oral multikinase inhibitor, has been used to treat radioactive iodine (RAI) therapy-refractory thyroid carcinoma in adults; however, pediatric experience is limited. Medical procedures and hospitalization for children with autism spectrum disorder may be challenging. CASE PRESENTATION: An 11-year-old boy with autism spectrum disorder and moderate intellectual impairment presented with dyspnea on exertion with thyroid carcinoma and diffuses lung metastases...
November 21, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29161093/b-cells-producing-type-i-interferon-modulate-macrophage-polarization-in-tuberculosis
#10
Alan Bénard, Imme Sakwa, Pablo Schierloh, André Colom, Ingrid Mercier, Ludovic Tailleux, Luc Jouneau, Pierre Boudinot, Talal Al-Saati, Roland Lang, Jan Rehwinkel, Andre G Loxton, Stefan He Kaufmann, Véronique Anton-Leberre, Anne O'Garra, Maria Del Carmen Sasiain, Brigitte Gicquel, Simon Fillatreau, Olivier Neyrolles, Denis Hudrisier
RATIONALE: In addition to their well-known function as antibody-producing cells, B lymphocytes can markedly influence the course of infectious or non-infectious diseases via antibody-independent mechanisms. In tuberculosis, B cells accumulate in lungs, yet their functional contribution to the host response remains poorly understood. OBJECTIVES: To document the role of B cells in tuberculosis in an unbiased manner. METHODS: We generated the transcriptome of B cells isolated from Mycobacterium tuberculosis (Mtb)-infected mice, and validated the identified key pathways using in vitro and in vivo assays...
November 21, 2017: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/29160001/low-level-laser-therapy-induces-increased-viability-and-proliferation-in-isolated-cancer-cells
#11
C Kara, H Selamet, C Gökmenoğlu, N Kara
OBJECTIVES: Low level laser therapy (LLLT), which stimulates natural biological processes in the application region, is frequently used in dental treatments. The aim of our study was to evaluate the effects of LLLT which could activate precancerous cells or increase existing cancerous tissue in case of clinically undetectable situations. MATERIALS AND METHODS: Saos-2 osteoblast-like osteosarcoma cells and A549 human lung carcinoma cells were used. Twenty-four hours after preparation of cell culture plates, laser irradiation was performed 1, 2 and 3 times according to the test groups using Nd:YAG laser with the power output 0...
November 20, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29159696/reflex-testing-of-speckled-cytoplasmic-patterns-observed-in-routine-ana-hep-2-indirect-immunofluorescence-with-a-multiplex-anti-synthetase-dot-blot-assay-a-multicentric-pilot-study
#12
Maria Infantino, Boaz Palterer, Roberta Biagiotti, Fabio Almerigogna, Maurizio Benucci, Arianna Damiani, Valentina Grossi, Annalisa Azzurri, Patrizia Casprini, Giovanni Bacci, Maria Grazia Giudizi, Mariangela Manfredi
Immunofluorescence on HEp2-cells is the standard diagnostic assay for the detection of anti-nuclear antibodies (ANA). Cytoplasmic speckled patterns are a common finding, and are associated with various antibodies, including anti-synthetase antibodies. However, classic ENA testing generally identifies only anti-Jo-1. Moreover, anti-synthetase syndrome is increasingly recognized as a pleomorphic entity, possibly presenting as isolated arthritis or interstitial lung disease. Sera referred for routine ANA testing were selected on the basis of the presence of a fine dense speckled cytoplasmic pattern (254 samples) and compared to control sera with negative cytoplasm (239 samples)...
November 21, 2017: Immunologic Research
https://www.readbyqxmd.com/read/29158156/anti-allergic-effects-of-sesquiterpene-lactones-from-saussurea-costus-falc-lipsch-determined-using-in-vivo-and-in-vitro-experiments
#13
Bo-Kyung Lee, Soo-Jin Park, So-Yeon Nam, Saeromi Kang, Jin Hwang, Seung-Jin Lee, Dong-Soon Im
ETHNOPHARMACOLOGICAL RELEVANCE: Saussurea costus (Falc.) Lipsch. root has been used in Asian traditional medicine for the treatment of asthma, rheumatism, and other conditions. S. costus extracts were shown to alleviate house dust mite-induced atopic-like dermatitis in Nc/Nga mice; besides, sesquiterpene lactones were isolated from S. costus extracts. AIMS OF THE STUDY: We aimed to investigate the effects of sesquiterpene lactones (alantolactone, costunolide, and dehydrocostuslactone) in allergic asthma using female Balb/c mice and rat RBL-2H3 mast cells...
November 17, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29157219/a-new-multiple-trauma-model-of-the-mouse
#14
Stefanie Fitschen-Oestern, Sebastian Lippross, Tim Klueter, Matthias Weuster, Deike Varoga, Mersedeh Tohidnezhad, Thomas Pufe, Stefan Rose-John, Hagen Andruszkow, Frank Hildebrand, Nadine Steubesand, Andreas Seekamp, Claudia Neunaber
BACKGROUND: Blunt trauma is the most frequent mechanism of injury in multiple trauma, commonly resulting from road traffic collisions or falls. Two of the most frequent injuries in patients with multiple trauma are chest trauma and extremity fracture. Several trauma mouse models combine chest trauma and head injury, but no trauma mouse model to date includes the combination of long bone fractures and chest trauma. Outcome is essentially determined by the combination of these injuries...
November 21, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/29155126/treatment-strategy-by-lactoperoxidase-and-lactoferrin-combination-immunomodulatory-and-antibacterial-activity-against-multidrug-resistant-acinetobacter-baumannii
#15
Likaa Mahdi, Nada Mahdi, Sana'a Al-Kakei, Hadeel Musafer, Ikbal Al-Joofy, Rajwa Essa, Luma Zwain, Istabreq Salman, Haifa Mater, Shaymaa Al-Alak, Rasha Al-Oqaili
Lactoperoxidase (Lpo) and Lactoferrin (Lf) were extracted from camel colostrum milk and purified. The antibacterial activity of the two purified proteins was estimated against 14 isolates of multidrug resistance Acinetobacter baumannii. A combination of Lpo and Lf exhibited bactericidal action against A. baumannii in vitro. A mouse model of acute A. baumannii pneumonia was improved. The injection of combined Lpo and Lf after infection leads to significant clearance of A. baumannii rates in lung as well as blood culture P < 0...
November 15, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29154953/correlation-between-cefepime-utilization-and-pseudomonas-aeruginosa-resistance-rate-to-beta-lactams-and-carbapenems-in-patients-with-healthcare-associated-infections
#16
Zorana M Djordjevic, Marko M Folic, Slobodan M Jankovic
OBJECTIVES: There is direct link between overutilization or abuse of antibiotics and P.aeruginosa resistance rate, but other factors could influence the resistance rate, too. The study aim was to observe changes of P.aeruginosa resistance rate in patients with hospital acquired infections (HAIs) after a period of cefepime unavailability in an intensive care unit (ICU). METHODS: The study was designed as retrospective observational analysis of trends in utilization of antibiotics and density of resistance to antibiotics...
November 14, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29153067/-detection-of-influenza-virus-infections-by-molecular-and-immunofluorescence-methods
#17
Deniz Yıldırım, Deniz Özdoğru Sağdıç, Buket Şeflek, Mehmet Çimentepe, İbrahim Bayram, Fügen Yarkın
Influenza virus infections are extremely important for human health due to the occurence of seasonal epidemics and pandemics worldwide. Influenza is associated with high morbidity and may result in serious complications such as life threatening viral or bacterial pneumonia. Especially, young children, older adults, patients with chronic diseases such as heart, lung, kidney, and diabetes and immunosuppressed people are at higher risk for complications and death from influenza virus infections. The aim of this study was to determine the incidence of influenza type A and B virus infections and influenza A virus subtypes in hospitalized patients with respiratory tract infections by real-time reverse transcriptase-polymerase chain reaction (RT-PCR, Sacace, Italy), conventional RT-PCR and direct immunofluorescence antibody (DFA, Argene SA, France) tests...
October 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29151505/isolated-adrenocorticotropin-deficiency-due-to-nivolumab-induced-hypophysitis-in-a-patient-with-advanced-lung-adenocarcinoma-a-case-report-and-literature-review
#18
Nobumasa Ohara, Kazumasa Ohashi, Toshiya Fujisaki, Chiyumi Oda, Yohei Ikeda, Yuichiro Yoneoka, Takehisa Hashimoto, Go Hasegawa, Kazuo Suzuki, Toshinori Takada
A 63-year-old Japanese woman with advanced lung adenocarcinoma developed isolated adrenocorticotropin deficiency caused by immune checkpoint inhibitor (ICI)-related hypophysitis following 8 months of nivolumab therapy. Prompt corticosteroid replacement therapy effectively relieved her secondary adrenal insufficiency symptoms and allowed her to pursue nivolumab therapy, which had been effective for the control of lung adenocarcinoma. Human leukocyte antigen (HLA) typing revealed the presence of the DRB1*04:05-DQA1*03:03-DQB1*04:01 haplotype, which is associated with susceptibility to autoimmune polyglandular syndrome with pituitary disorder in the Japanese population...
November 20, 2017: Internal Medicine
https://www.readbyqxmd.com/read/29151418/-vegf165-transfected-endothelial-progenitor-cells-mediated-by-lentivirus-alleviated-ali-in-rats
#19
Zhaohui He, Huiwei He, Yuanhua Lu, Zhi Chen, Fanghua Xu, Rongsheng Wang, Chunli Yang
OBJECTIVE: To investigate the protective effects of vascular endothelial growth factor-165 (VEGF165) transfected the endothelial progenitor cells (EPCs) mediated by lentivirus on acute lung injury (ALI) in rats. METHODS: The mononuclear cells from the male Sprague-Dawley (SD) rats were isolated and cultured to get the EPCs for study. The lentivirus vector carrying the human VEGF165 gene was constructed. According to the random number table method, 90 male SD rats were divided into ALI model group, phosphate buffer solution (PBS) group, EPCs treatment group, none transfected EPCs treatment group and VEGF165 transfected EPCs treatment group, and the rats in each group were subdivided into 4, 12 and 48 hours subgroups, with 6 rats in each subgroup...
November 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29151278/tetrahydrobiopterin-bh4-targeting-endothelial-nitric-oxide-synthase-as-a-potential-therapy-for-pulmonary-hypertension
#20
Bahaa N Francis, Maram Salameh, Rola Khamisy-Farah, Raymond Farah
PURPOSE: Pulmonary Hypertension (PH) is complex disease which is associated with endothelial and cardiac dysfunction. Tetrahydrobiopterin (BH4 ) regulates endothelial nitric oxide synthase (eNOS) to produce nitric oxide rather than superoxide which maintains normal endothelial and cardiac function. This study explores the therapeutic potential of BH4 in experimental PH. METHODS: Monocrotaline-induced PH in rats and Hph-1 deficiency in mice were used for animal experiments...
November 19, 2017: Cardiovascular Therapeutics
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