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Lara Faggi, Andrea Giustina, Giovanni Tulipano
For a few years we have been investigating AMP-activated protein kinase (AMPK) as a target for drug therapy of GH-secreting pituitary adenomas. Aim of this study was to investigate the direct effects of metformin, which causes AMPK activation in different cell types, on rat pituitary adenoma cell growth and on related cell signalling pathways. Our results suggest that metformin can exert a growth-inhibitory activity in rat pituitary tumor cells mediated by AMPK activation, although multiple mechanisms are most likely involved...
September 27, 2017: Molecular and Cellular Endocrinology
Fiorella Faggi, Silvia Codenotti, Pietro Luigi Poliani, Manuela Cominelli, Nicola Chiarelli, Marina Colombi, Marika Vezzoli, Eugenio Monti, Federica Bono, Giovanni Tulipano, Chiara Fiorentini, Alessandra Zanola, Harriet P Lo, Robert G Parton, Charles Keller, Alessandro Fanzani
The purpose of this study was to investigate whether MURC/cavin-4, a plasma membrane and Z-line associated protein exhibiting an overlapping distribution with Caveolin-3 (Cav-3) in heart and muscle tissues, may be expressed and play a role in rhabdomyosarcoma (RMS), an aggressive myogenic tumor affecting childhood. We found MURC/cavin-4 to be expressed, often concurrently with Cav-3, in mouse and human RMS, as demonstrated through in silico analysis of gene datasets and immunohistochemical analysis of tumor samples...
2015: PloS One
G Tulipano, L Faggi, A Cacciamali, M Spinello, D Cocchi, A Giustina
AMP-activated protein kinase (AMPK) is activated under conditions that deplete cellular ATP levels and elevate AMP levels. We have recently shown that AMPK can represent a valid target for improving the medical treatment of growth hormone (GH)-secreting pituitary adenomas and the effects of its activation or inhibition in pituitary tumour cells are worthy of further characterisation. We aimed to determine whether AMPK may have a role in combined antiproliferative therapies based on multiple drugs targeting cell anabolic functions at different levels in pituitary tumour cells to overcome the risk of cell growth escape phenomena...
January 2015: Journal of Neuroendocrinology
Giovanni Tulipano, Lara Faggi, Andrea Cacciamali, Maurizio Spinello, Daniela Cocchi, Andrea Giustina
Estrogen receptor α has a role in regulating rat somatolactotroph tumor cell growth (GH3 cells). AMP-activated protein kinase (AMPK) is a metabolic checkpoint which is able to negatively regulate intracellular signaling downstream of growth factors receptors in conditions increasing cellular AMP levels. We have recently reported on the role of AMPK activation in affecting viability and proliferation of GH3 cells. In the present study, we investigated the interplay between ER- and AMPK-pathways. Results can be regarded as relevant to the development of novel multi-targeted pharmacological therapies against pituitary tumors...
June 2014: Pituitary
Giovanni Tulipano, Lara Faggi, Marco Losa, Pietro Mortini, Maurizio Spinello, Valeria Sibilia, Francesca Pagani, Daniela Cocchi, Andrea Giustina
We investigated the effects of the AMPK activator AICAR as compared to somatostatin-14 on cell viability and GH secretion in human GH-secreting pituitary adenomas in vitro and in rat GH3 cells. Overnight treatment with AICAR increased phospho-(threonine-172) AMPK levels (activated AMPK) in cultured human adenomas. As to the effects on cell viability, four adenomas out of 15 were responsive to AICAR (0.4mM) and five adenomas were responsive to SS-14 (100 nM). One adenoma was responsive to both somatostatin and AICAR...
January 30, 2013: Molecular and Cellular Endocrinology
Giovanni Tulipano, Lara Faggi, Valeria Sibilia, Andrea Giustina
AMP-activated protein kinase (AMPK), an enzyme functioning as a cellular sensor of low energy, stores and promotes adaptive changes in growth, differentiation, and metabolism. While AMPK is primarily thought of as a regulator of systemic metabolism, it has been clearly established that it also has a role in the regulation of cell growth and may be a therapeutic target for proliferative disorders. Growth hormone (GH) secretion from the anterior pituitary and GH-induced synthesis and release of insulin-like-growth-factor-1 (IGF-1) from the liver determine linear growth before puberty...
October 2012: Endocrine
Valeria Sibilia, Francesca Pagani, Emanuela Mrak, Elisa Dieci, Giovanni Tulipano, Francesco Ferrucci
Recent research suggests a role for ghrelin in the modulation of inflammatory disorders. However, the type of ghrelin receptor (GHS-R) involved in both the anti-inflammatory and anti-hyperalgesic actions of ghrelin remains to be characterized. In this study, we examined whether the inhibitory effect of ghrelin in the development of hyperalgesia and edema induced by intraplantar carrageenan administration depends on an interaction with GHS-R1a. Both central (1 nmol/rat, i.c.v.) and peripheral (40 nmol/kg, i...
October 2012: Amino Acids
Esme Fuller-Thomson, Maressa J Tulipano, Michael Song
BACKGROUND: Stroke survivors often experience poststroke depression and suicidal ideation. PURPOSE: to determine the frequency and odds ratio of depression and suicidal ideation among stroke survivors, in comparison to those without stroke, and to identify demographic factors associated with elevated odds of depression and suicidal ideation among stroke survivors. METHODS: Secondary analysis of the Canadian Community Health Survey, a population-based sample...
April 2012: International Journal of Stroke: Official Journal of the International Stroke Society
Giovanni Tulipano, Valeria Sibilia, Anna Maria Caroli, Daniela Cocchi
Preclinical and clinical studies suggest that whey proteins can reduce postprandial glucose levels and stimulate insulin release in healthy subjects and in subjects with type 2 diabetes by reducing dipeptidyl peptidase-4 (DPP-4) activity in the proximal bowel and hence increasing intact incretin levels. Our aim was to identify DPP-4 inhibitors among short peptides occurring in hydrolysates of β-lactoglobulin, the major whey protein found in the milk of ruminants. We proved that the bioactive peptide Ile-Pro-Ala can be regarded as a moderate DPP-4 inhibitor...
April 2011: Peptides
Giovanni Tulipano, Michela Giovannini, Maurizio Spinello, Valeria Sibilia, Andrea Giustina, Daniela Cocchi
AMP-activated protein kinase (AMPK) is activated under conditions that deplete cellular ATP and elevate AMP levels such as glucose deprivation and hypoxia. The AMPK system is primarily thought of as a regulator of metabolism and cell proliferation. Little is known about the regulation and the effects of AMPK in somatotroph cells. We present results from "in vitro" studies showing that AMPK activity has a role in regulating somatotroph function in normal rat pituitary and is a promising target for the development of new pharmacological treatments affecting cell proliferation and viability of pituitary adenomatous cells...
September 2011: Pituitary
Valeria Sibilia, Francesca Pagani, Ilaria Bulgarelli, Giovanni Tulipano, Roberta Possenti, Francesca Guidobono
TLQP-21, a vgf-derived peptide modulates gastric emptying and prevents ethanol-induced gastric lesions in rats. However, it remains to be studied whether or not TLQP-21 affects gastric acid secretion. In this study, we evaluated the effects of central (0.8-8 nmol/rat) or peripheral (48-240 nmol/kg, intraperitoneally) TLQP-21 administration on gastric acid secretion in pylorus-ligated rats. The mechanisms involved in such activity were also examined. Central TLQP-21 injection significantly reduced gastric acid volume and dose-dependently inhibited total acid output (ED(50) = 2...
April 2012: Amino Acids
Debby F Mir, Yoram Finkelstein, Gayle D Tulipano
Children, mainly infants, are especially vulnerable to pesticides, as a result of physiological factors which facilitate absorption of chemicals and limit the ability to detoxify and eliminate them. Moreover, children exhibit mouthing activity with pesticide contaminated objects. Therefore, the rapid course of growth and development creates a time-frame of unique vulnerability, where exposed children are prone to develop delayed neurotoxic brain disorders. Parents, childcare workers and staff are generally untrained in using pesticides and may not follow instructions or consider safer alternatives in efforts to provide a sanitary pest-free environment...
September 2010: Neurotoxicology
Giovanni Tulipano, Omar Bulgari, Stefania Chessa, Alessandro Nardone, Daniela Cocchi, Anna Caroli
Casein phosphopeptides (CPPs) obtained by enzymatic hydrolysis in vitro of caseins, have been shown to enhance calcium solubility and to increase the calcification of embryonic rat bones in their diaphyseal area. Little is known about the direct effects of CPPs on cultured osteoblastic cells. Calcium in the microenvironment surrounding bone cells is not only important for the mineralization of the extracellular matrix, but it is believed to provide preosteblasts with a signal that modulates their proliferation and differentiation...
February 25, 2010: Regulatory Peptides
Daniela Cocchi, Giovanni Tulipano, Alessandra Colciago, Valeria Sibilia, Francesca Pagani, Daniela Viganò, Tiziana Rubino, Daniela Parolaro, Patrizia Bonfanti, Anita Colombo, Fabio Celotti
Polychlorinated biphenyls (PCBs) are pollutants detected in animal tissues and breast milk. The experiments described in the present paper were aimed at evaluating whether the four PCB congeners most abundant in animal tissues (PCB-138, -153, -180 and -126), administered since fetal life till weaning, can induce long-term alterations of GH-axis activity and bone mass in the adult rat. We measured PCB accumulation in rat brain and liver, somatic growth, pituitary GH expression and plasma hormone concentrations at different ages...
June 1, 2009: Toxicology and Applied Pharmacology
Giovanni Tulipano, PierFranco Spano, Daniela Cocchi
The aim of our study was to investigate the direct effects of atypical antipsychotics on muscle cell functions in order to ascertain the diabetic liability of these drugs. We investigated the effects of olanzapine, clozapine and alpha-methyl-5-hydroxytryptamine on basal glucose uptake and glucose uptake in response to insulin using in vitro cultures of mouse skeletal muscle satellite cells (C2C12). We extended our study to the effects of these compounds on cell proliferation, survival and differentiation into myotubes and on the growth of differentiated myotubes...
September 24, 2008: Molecular and Cellular Endocrinology
Angelica Tulipano, Giacinto Donvito, Flavio Licciulli, Giorgio Maggi, Andreas Gisel
BACKGROUND: To date more than 2,1 million gene products from more than 100000 different species have been described specifying their function, the processes they are involved in and their cellular localization using a very well defined and structured vocabulary, the gene ontology (GO). Such vast, well defined knowledge opens the possibility of compare gene products at the level of functionality, finding gene products which have a similar function or are involved in similar biological processes without relying on the conventional sequence similarity approach...
2007: BMC Bioinformatics
Giovanni Tulipano, John E Taylor, Heather A Halem, Rakesh Datta, Jesse Z Dong, Michael D Culler, Irene Bianchi, Daniela Cocchi, Andrea Giustina
Glucocorticoids are important immunosuppressive hormones; these steroids also inhibit somatic growth by decreased growth hormone (GH) secretion and induced protein catabolism. The ability of ghrelin, the endogenous ligand for the GHS-1a receptor, to increase body weight is attributed to a combination of enhanced food intake, increased gastric emptying and increased food assimilation, coupled with potent GH releasing activity. The aim of the present study was to evaluate the ability of a full-length, metabolically stabilized ghrelin agonist, BIM-28125, to reverse the dexamethasone-induced decrease of growth rate of prepubertal Sprague-Dawley male rats...
2007: Pituitary
Giovanni Tulipano, Stefan Schulz
The experimental data reviewed in the present paper deal with the molecular events underlying the agonist-dependent regulation of the distinct somatostatin receptor subtypes and may suggest important clues about the clinical use of somatostatin analogs with different pattern of receptor specificity for the in vivo targeting of tumoral somatostatin receptors. Somatostatin receptor subtypes are characterized by differential beta-arrestin trafficking and endosomal sorting upon agonist binding due, at least in part, to the differences in their C-terminal tails...
April 2007: European Journal of Endocrinology
Giovanni Tulipano, Cristina Rizzetti, Irene Bianchi, Alessandro Fanzani, PierFranco Spano, Daniela Cocchi
BACKGROUND/AIMS: To our knowledge, a suitable animal model to investigate how atypical antipsychotics may induce diabetes in patients has not received much attention. METHODS: We investigated the effects of acute as well as subchronic administration of clozapine on food intake, body weight gain, glucose tolerance and insulin secretion in response to glucose in Sprague-Dawley rats. We then evaluated the effects of clozapine on corticosterone secretion and 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD-1) and phosphoenolpyruvate carboxykinase (PEPCK) expression in the liver...
2007: Neuroendocrinology
P Karina Tulipano, Ying Tao, William S Millar, Pat Zanzonico, Katherine Kolbert, Hua Xu, Hong Yu, Lifeng Chen, Yves A Lussier, Carol Friedman
Molecular imaging is at the crossroads of genomic sciences and medical imaging. Information within the molecular imaging literature could be used to link to genomic and imaging information resources and to organize and index images in a way that is potentially useful to researchers. A number of natural language processing (NLP) systems are available to automatically extract information from genomic literature. One existing NLP system, known as BioMedLEE, automatically extracts biological information consisting of biomolecular substances and phenotypic data...
June 2007: Journal of Biomedical Informatics
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