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https://www.readbyqxmd.com/read/28434192/modified-ipswich-procedure-patient-perceived-benefit-and-surgical-outcomes-in-a-series-of-ten-patients
#1
Blazej Staniszewski, Richard Green, Graeme Sharpe, Paul White
Acquired nasolacrimal duct obstruction (NLO) causes symptoms of epiphora which can be associated with stickiness, dacryocystitis and interference with tear film causing visual blurring [1]. The annual incidence rate of NLO is 20 per 100,000, with the majority of patients being females above the age of 66 years [2]. While the aetiology is usually unknown, the site of obstruction is more commonly distal and invariably amenable to treatment by DCR surgery [3-4]. Proximal NLO at the level of the cancaliculi is less common but may still be treatable by extended DCR techniques including canalulostomy...
April 23, 2017: Clinical Otolaryngology
https://www.readbyqxmd.com/read/28434164/microglia-loss-contributes-to-the-development-of-major-depression-induced-by-different-types-of-chronic-stresses
#2
Lijuan Tong, Yu Gong, Peng Wang, Wenfeng Hu, Jili Wang, Zhuo Chen, Wei Zhang, Chao Huang
Recently, the loss and dystrophy of hippocampal microglia induced by chronic unpredictable stress (CUS) has been reported to mediate the development of major depression in mice whose microglial cells were labeled with enhanced green fluorescent protein-conjuncted-CX3C receptor type 1. However, whether this happens in endogenous microglia with no genetic intervention remains unclear. Here, we addressed this issue in mice treated with different types of chronic stresses, including the CUS, chronic restraint stress (CRS) and chronic social defeat stress (CSDS)...
April 22, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28434066/toxicity-and-genotoxicity-of-three-antimicrobials-commonly-used-in-veterinary-medicine
#3
Anahí Magdaleno, Sofía Carusso, Juan Moretton
The toxicity of chlortetracycline (CTC), oxytetracycline (OTC) and enrofloxacin (ENF) was tested on two green algal species: the international standard Pseudokirchneriella subcapitata and the native Argentine species Ankistrodesmus fusiformis. All three antibiotics inhibited the algal growth. The most sensitive species was P. subcapitata, for which the EC50 for CTC, OTC and ENF were 1.19 ± 0.53, 0.92 ± 0.30 and 5.18 ± 3.80 mg L(-1), respectively. The EC50 for A. fusiformis, were 3.23 ± 0...
April 22, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28433940/a-novel-dual-ratiometric-response-fluorescent-probe-for-so2-clo-detection-in-cells-and-in%C3%A2-vivo-and-its-application-in-exploring-the-dichotomous-role-of-so2-under-the-clo-induced-oxidative-stress
#4
Kun Dou, Qiang Fu, Guang Chen, Fabiao Yu, Yuxia Liu, Ziping Cao, Guoliang Li, Xianen Zhao, Lian Xia, Lingxin Chen, Hua Wang, Jinmao You
Intracellular reactive sulfur species and reactive oxygen species play vital roles in immunologic mechanism. As an emerging signal transmitter, SO2 can be generated as the anti-oxidant, while SO2 is also a potential oxidative stress-inducer in organism. Aiming to elucidate in-depth the dichotomous role of SO2 under oxidative stress, we designed a dual-response fluorescent probe that enabled the respective or successive detection of SO2 and ClO(-). The probe itself emits the red fluorescence (625 nm) which can largely switch to blue (410 nm) and green fluorescence (500 nm) respectively in response to SO2 and ClO(-), allowing the highly selective and accurate ratiometric quantification for both SO2 and ClO(-) in cells...
April 14, 2017: Biomaterials
https://www.readbyqxmd.com/read/28433937/perturbation-effect-of-reduced-graphene-oxide-quantum-dots-rgoqds-on-aryl-hydrocarbon-receptor-ahr-pathway-in-zebrafish
#5
Jing-Hui Zhang, Tai Sun, Aping Niu, Yu-Mei Tang, Shun Deng, Wei Luo, Qun Xu, Dapeng Wei, De-Sheng Pei
Graphene quantum dots (GQDs) has been widely used in enormous fields, however, the inherent molecular mechanism of GQDs for potential risks in biological system is still elusive to date. In this study, the outstanding reduced graphene quantum dots (rGOQDs) with the QY as high as 24.62% were successfully synthesized by the improved Hummers method and DMF hydrothermal treatment approach. The rGOQDs were N-doped photoluminescent nanomaterials with functional groups on the surface. The fluorescent bio-imaging was performed by exposing zebrafish in different concentrations of the as-prepared rGOQDs, and the distribution of rGOQDs was successfully observed...
April 15, 2017: Biomaterials
https://www.readbyqxmd.com/read/28433936/sustained-viral-gene-delivery-from-a-micro-fibrous-elastomeric-cardiac-patch-to-the-ischemic-rat-heart
#6
Xinzhu Gu, Yasumoto Matsumura, Ying Tang, Souvik Roy, Richard Hoff, Bing Wang, William R Wagner
Biodegradable and elastomeric patches have been applied to the surface of infarcted hearts as temporary mechanical supports to effectively alter adverse left ventricular remodeling processes. In this report, recombinant adeno-associated virus (AAV), known for its persistent transgene expression and low pathogenicity, was incorporated into elastomeric polyester urethane urea (PEUU) and polyester ether urethane urea (PEEUU) and processed by electrospinning into two formats (solid fibers and core-sheath fibers) designed to influence the controlled release behavior...
April 14, 2017: Biomaterials
https://www.readbyqxmd.com/read/28433837/internal-maxillary-bypass-for-complex-pediatric-aneurysms
#7
Long Wang, Shuaibin Lu, Hai Qian, Xiang'en Shi
BACKGROUND: Complex pediatric aneurysms (PAs) are an unusual clinicopathologic entity, and data remain limited regarding the use of a bypass procedure to treat this formidable disorder. METHODS: Internal maxillary artery (IMA) to middle cerebral artery (MCA) bypass with radial artery graft (RAG) was utilized to isolate PAs. Bypass patency and aneurysm stability were evaluated using intraoperative Doppler ultrasound, indocyanine green videoangiography and postoperative angiography...
April 19, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28433694/bacteroidales-markers-for-microbial-source-tracking-in-southeast-asia
#8
Jean Pierre Nshimyimana, Mercedes C Cruz, R Janelle Thompson, Stefan Wuertz
The island city country of Singapore served as a model to validate the use of host-associated Bacteroidales 16S rRNA gene marker assays for identifying sources of fecal pollution in the urban tropical environment of Southeast Asia. A total of 295 samples were collected from sewage, humans, domesticated animals (cats, dogs, rabbits and chicken), and wild animals (birds, monkeys and wild boars). Samples were analyzed by real time PCR using five human-associated assays (HF183-SYBR Green, HF183, BacHum, BacH and B...
April 10, 2017: Water Research
https://www.readbyqxmd.com/read/28433693/cyanotoxins-which-detection-technique-for-an-optimum-risk-assessment
#9
Virginie Gaget, Melody Lau, Barbara Sendall, Suzanne Froscio, Andrew R Humpage
The presence of toxigenic cyanobacteria (blue-green algae) in drinking water reservoirs poses a risk to human and animal health worldwide. Guidelines and health alert levels have been issued in the Australian Drinking Water Guidelines for three major toxins, which are therefore the subject of routine monitoring: microcystin, cylindrospermopsin and saxitoxin. While it is agreed that these toxic compounds should be monitored closely, the routine surveillance of these bioactive chemicals can be done in various ways and deciding which technique to use can therefore be challenging...
April 10, 2017: Water Research
https://www.readbyqxmd.com/read/28433643/green-light-for-lipid-fingerprinting
#10
Richard P Haslam, Ivo Feussner
The use of targeted lipidomic approaches for the analysis of plant lipids has steadily increased during recent years. We review recent developments of these methods and suggest the introduction of discovery lipidomics as additional approach through a new workflow, lipid fingerprinting, that integrates the advantages of shotgun lipidomics (quantitative data) with LC-MS-based strategies (higher resolution and/or coverage). This article is part of a Special Issue entitled:BBALIP_Lipidomics Opinion Articles edited by Sepp Kohlwein...
April 19, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28433445/parapapillary-choroidal-microvasculature-dropout-in-glaucoma-a-comparison-between-optical-coherence-tomography-angiography-and-indocyanine-green-angiography
#11
Eun Ji Lee, Kyoung Min Lee, Seung Hyen Lee, Tae-Woo Kim
PURPOSE: To investigate whether the parapapillary choroidal microvasculature dropout (MvD) determined by optical coherence tomography angiography (OCTA) in glaucomatous eyes indicates a true perfusion defect and whether the MvD accurately represents the area of nonperfusion. DESIGN: Observational case series. PARTICIPANTS: Thirty primary open-angle glaucoma (POAG) patients with choroidal MvD as determined by OCTA and 13 POAG patients without this dropout...
April 19, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28433367/abnormal-implantation-after-fresh-and-frozen-in%C3%A2-vitro-fertilization-cycles
#12
Erica T Wang, Anupama S Q Kathiresan, Catherine Bresee, Naomi Greene, Carolyn Alexander, Margareta D Pisarska
OBJECTIVE: To determine whether fresh embryo transfers are at a higher risk of abnormal implantation compared with frozen embryo transfers while accounting for the embryo stage at transfer. DESIGN: Retrospective cohort study. SETTING: Not applicable. PATIENT(S): We used data from the Society for Assisted Reproductive Technologies to identify all fresh and frozen autologous IVF cycles from 2004-2013 resulting in a positive pregnancy test...
April 19, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28433218/cardiac-arrhythmia-after-open-thoracoabdominal-aortic-aneurysm-repair
#13
Ahmet Dolapoglu, Irina V Volguina, Matt D Price, Susan Y Green, Joseph S Coselli, Scott A LeMaire
BACKGROUND: Cardiac arrhythmias commonly arise after cardiac surgery and are associated with poor prognosis. In thoracoabdominal aortic aneurysm (TAAA) repair, these complications are poorly understood. We assessed characteristics, incidence, outcomes, and potential predictors of postoperative arrhythmia (PA) after open TAAA repair. METHODS: From 2010 to 2014, 403 consecutive open TAAA replacement operations were performed in patients without preoperative cardiac rhythm abnormalities at a single tertiary center...
April 19, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28433060/enhanced-photoresponsive-polyethyleneimine-citric-acid-co-carbonized-dots-for-facile-and-selective-sensing-and-intracellular-imaging-of-cobalt-ions-at-physiologic-ph
#14
Wen-Sheng Zou, Qing-Chun Zhao, Jun Zhang, Xiao-Ming Chen, Xiu-Fang Wang, Lin Zhao, Shao-Hua Chen, Ya-Qin Wang
Whether as an important biological element or as a radioactive source/medicine, the monitoring of trace levels of cobalt ions (Co) has become a non-negligible factor for human health and green environment. Current technologies for the detection of Co are cost-expensive and time-consuming, and require cumbersome sample pretreatment process. Herein a novel sensing platform has been developed for Co detection based on the quenching of the enhanced fluorescence signal of polyamine functionalized C-dots. Amine groups at the surface of the C-dots can capture Zn(2+)/Cd(2+) to form coordination compound, which can inhibit the photoinduced electron transfer pathways of C-dots and then induce the fluorescence enhancement of the C-dots by ∼80% margin...
June 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28432996/toxicological-effects-of-phenol-on-four-marine-microalgae
#15
Weiyan Duan, Fanping Meng, Yufei Lin, Guoshan Wang
The toxic effects of phenol on four marine microalgae (Dunaliella salina, Platymonas subcordiformis, Phaeodactylum tricornutum Bohlin, and Skeletonema costatum) were evaluated. The 96h EC50 values were 72.29, 92.97, 27.32, and 27.32mgL(-1), respectively, which were lower than those values of freshwater microalgae reported in the literature. During a 96-h exposure to a sub-lethal concentration of phenol (1/2 96h EC50) with green alga (D. salina) and diatom (S. costatum), reactive oxygen species (ROS) accumulation, and chlorophyll a (Chl a) content decrease were simultaneously observed in diatom cells after 48h treatment...
April 13, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28432978/cadmium-hampers-salt-tolerance-of-sesuvium-portulacastrum
#16
Mariem Wali, Soledad Martos, Laura Pérez-Martín, Chedly Abdelly, Tahar Ghnaya, Charlotte Poschenrieder, Benet Gunsé
It is well known that salinity reduces cadmium toxicity in halophytes. However, the possible interference of Cd with the mechanisms of salt tolerance is poorly explored. The aim of this study was to see whether Cd affects salt tolerance mechanisms in the halophyte Sesuvium portulacastrum. S. portulacastrum plants obtained from cuttings were grown in hydroponics for 3 weeks and then exposed to low (0.09 mM) or moderate (200 mM) NaCl concentrations, alone or in combination with 25 μM CdCl2. Microscopy observation revealed two strategies of salt tolerance: euhalophytism and secretion of salt by bladder cells...
April 13, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28432554/epigallocatechin-gallate-protects-beas-2b-cells-from-lipopolysaccharide-induced-apoptosis-through-upregulation-of-gastrin-releasing-peptide
#17
Peethambaran Divya, Bijesh Puthusseri, Denny Joseph Kollareth Manual, Mohammed Azharuddin Savanur
Gastrin-releasing peptide (GRP) plays a major role in the development and maintenance of lung epithelial cells by promoting cell division, whereas its suppression causes growth arrest and apoptosis. The present study shows that human bronchial epithelial BEAS-2B cells challenged with lipopolysaccharide (LPS), an endotoxin from gram-negative bacteria, downregulated GRP expression and induced apoptosis via upregulation of p53 and active caspase-3, signifying the importance of GRP in lung epithelial cell survival...
April 21, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28432498/co-cultivation-of-chlamydomonas-reinhardtii-with-azotobacter-chroococcum-improved-h2-production
#18
Lili Xu, Xianglong Cheng, Shuangxiu Wu, Quanxi Wang
OBJECTIVES: To improve H2 production, the green algae Chlamydomonas reinhardtii cc849 was co-cultured with Azotobacter chroococcum. RESULTS: The maximum H2 production of the co-culture was 350% greater than that of the pure algal cultures under optimal H2 production conditions. The maximum growth and the respiratory rate of the co-cultures were about 320 and 300% of the controls, and the dissolved O2 of co-cultures was decreased 74%. Furthermore, the in vitro maximum hydrogenase activity of the co-culture was 250% greater than that of the control, and the in vivo maximum hydrogenase activity of the co-culture was 1...
April 21, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28432348/white-spot-syndrome-virus-vp28-specific-double-stranded-rna-provides-protection-through-a-highly-focused-sirna-population
#19
Pål Nilsen, Marius Karlsen, Kallaya Sritunyalucksana, Siripong Thitamadee
Several studies have demonstrated that injection of double-stranded RNAs (dsRNA) homologous to mRNA for the white spot syndrome virus (WSSV) viral protein 28 (VP28) can induce protection in shrimp against WSSV through RNA interference (RNAi). In comparison to shrimp injected with either PBS or a green fluorescent protein (GFP) nonspecific dsRNA, we obtained nearly complete protection against WSSV infection in shrimp injected with VP28 dsRNA. Upregulation of host genes associated with small RNA silencing was measured 48 hours post treatment in groups injected with dsRNA, and although the VP28-treated group remained moderately upregulated after challenge with WSSV, many-fold higher induction was observed in both control groups reflecting the ongoing viral infection...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28432320/formation-of-cuo-nano-flowered-surfaces-via-submerged-photo-synthesis-of-crystallites-and-their-antimicrobial-activity
#20
Fumika Nishino, Melbert Jeem, Lihua Zhang, Kazumasa Okamoto, Satoshi Okabe, Seiichi Watanabe
We report the fabrication of flower-like CuO nanostructured surfaces via submerged photo-synthesis of crystallites (SPSC), which requires only UV illumination in neutral water. In this paper, we discuss the reaction mechanism of the photochemical formation of the SPSC-fabricated CuO nanostructures in detail based on surface microstructural analyses and a radiation-chemical consideration with additional gamma-ray irradiation. Since the SPSC method for surface nanostructural fabrication can work at low temperatures at atmospheric pressure without using harmful substances, it is a potential fabrication method for green nanotechnology applications...
April 21, 2017: Scientific Reports
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