Eva Harlacher, Corinna Schulte, Sonja Vondenhoff, Philippe Schmitt-Kopplin, Philippe Diederich, Christian Hemmers, Julia Moellmann, Julia Wollenhaupt, Rogier Veltrop, Erik Biessen, Michael Lehrke, Björn Peters, Georg Schlieper, Christoph Kuppe, Jürgen Floege, Vera Jankowski, Nikolaus Marx, Joachim Jankowski, Heidi Noels
Chronic kidney disease (CKD) significantly increases cardiovascular risk and mortality, and the accumulation of uremic toxins in the circulation upon kidney failure contributes to this increased risk. We thus performed a screening for potential novel mediators of reduced cardiovascular health starting from dialysate obtained after hemodialysis of patients with CKD. The dialysate was gradually fractionated to increased purity using orthogonal chromatography steps, with each fraction screened for a potential negative impact on the metabolic activity of cardiomyocytes using a high-throughput MTT-assay, until ultimately a highly purified fraction with strong effects on cardiomyocyte health was retained...
2024: Frontiers in Cardiovascular Medicine