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https://www.readbyqxmd.com/read/29045722/subjective-age-and-cystatin-c-among-older-adults
#1
Yannick Stephan, Angelina R Sutin, Antonio Terracciano, Shevaun Neupert
Objective: Cystatin C (CysC) is a marker of kidney function that is relevant for the health and cognition of older adults. Little is known about the link between psychological factors and CysC. Therefore, the present study examined whether subjective age is related to CysC level and changes in CysC over time. Method: Participants were 5,066 individuals drawn from the Health and Retirement Study aged from 50 to 107 years (60% women, mean age = 69.36 years, SD = 9...
October 16, 2017: Journals of Gerontology. Series B, Psychological Sciences and Social Sciences
https://www.readbyqxmd.com/read/29045709/joint-effect-of-physical-activity-and-sedentary-behaviour-on-cardiovascular-risk-factors-in-chilean-adults
#2
Carlos Cristi-Montero, Lewis Steell, Fanny Petermann, Alex Garrido-Méndez, Ximena Díaz-Martínez, Carlos Salas-Bravo, Rodrigo Ramirez-Campillo, Cristian Alvarez, Fernando Rodriguez, Nicolas Aguilar-Farias, Maria A Martinez, Ana M Leiva, Felipe Poblete-Valderrama, Naomi D Willis, Carlos A Celis-Morales
Background: To investigate the associations between combined categories of moderate-to-vigorous physical activity (MVPA) and sedentary behaviour (SB) with markers of adiposity and cardiovascular risk in adults. Methods: Overall, 5040 participants (mean age 46.4 years and 59.3% women) from the cross-sectional Chilean National Health Survey 2009-2010 were included in this study. MVPA and SB were measured using the Global Physical Activity questionnaire. Four categories were computed using MVPA- and SB-specific cut-offs ('High-SB & Active', 'Low-SB & Active', 'High-SB & Inactive' and 'Low-SB & Inactive')...
October 17, 2017: Journal of Public Health
https://www.readbyqxmd.com/read/29045151/dynamic-blue-light-inducible-t7-rna-polymerases-opto-t7rnaps-for-precise-spatiotemporal-gene-expression-control
#3
Armin Baumschlager, Stephanie K Aoki, Mustafa Khammash
Light has emerged as a control input for biological systems due to its precise spatiotemporal resolution. The limited toolset for light control in bacteria motivated us to develop a light-inducible transcription system that is independent from cellular regulation through the use of an orthogonal RNA polymerase. Here, we present our engineered blue light-responsive T7 RNA polymerases (Opto-T7RNAPs) that show properties such as low leakiness of gene expression in the dark state, high expression strength when induced with blue light, and an inducible range of more than 300-fold...
October 18, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29045133/the-product-of-yersinia-pseudotuberculosis-mcc-operon-is-a-peptide-cytidine-antibiotic-activated-inside-producing-cells-by-the-tldd-e-protease
#4
Darya Tsibulskaya, Olga Mokina, Alexey Kulikovsky, Julia Piskunova, Konstantin Severinov, Marina Serebryakova, Svetlana Dubiley
Microcin C is a heptapeptide-adenylate antibiotic produced by some strains of Escherichia coli. Its peptide part is responsible for facilitated transport inside sensitive cells where it is proteolysed with release of a toxic warhead - a non-hydrolysable aspartamidyl-adenylate, which inhibits aspartyl-tRNA synthetase. Recently, a microcin C homolog from Bacillus amyloliquefaciens containing a longer peptide part modified with carboxymethyl-cytosine instead of adenosine was described but no biological activity of this compound was revealed...
October 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29044950/biochemical-characterization-of-argininosuccinate-lyase-from-m-tuberculosis-significance-of-a-c-terminal-cysteine-in-catalysis-and-thermal-stability
#5
Archita Mishra, Avadhesha Surolia
Arginine biosynthesis pathway is crucial to the survival and pathogenesis of Mycobacterium tuberculosis (Mtb). Arginine is a critical amino acid that contributes to the inflection of cellular immune responses during pathogenesis. Argininosuccinate lyase from Mtb (MtArgH), the last enzyme in the pathway, catalyzes the production of arginine from argininosuccinic acid. MtArgH is an essential enzyme for the growth and survival of M. tuberculosis. We biochemically characterized MtArgH and deciphered the role of a previously unexplored cysteine (Cys(441) ) residue at the C-terminal region of the protein...
October 16, 2017: IUBMB Life
https://www.readbyqxmd.com/read/29043767/synthesis-of-ag3po4-crystals-with-tunable-shapes-for-facet-dependent-optical-property-photocatalytic-activity-and-electrical-conductivity-examinations
#6
Meng-Shan Hsieh, Huang-Jen Su, Pei-Lun Hsieh, Yun-Wei Chiang, Michael H Huang
This work has developed conditions for the synthesis of Ag3PO4 cubes, rhombic dodecahedra, {100}-truncated rhombic dodecahedra, tetrahedra, and tetrapods by tuning the amount of NH4NO3, NaOH, AgNO3, and K2HPO4 solutions added. Use of a lesser amount of AgNO3 solution can form much smaller rhombic dodecahedra and tetrahedra. Sub-micrometer-sized Ag3PO4 cubes and rhombic dodecahedra with sizes larger than 300 nm do not exhibit optical size effect, but ~290 nm rhombic dodecahedra show a smaller band gap value than that for larger cubes, and tetrahedra show the most blue-shifted absorption edge...
October 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29042637/the-polyglutamine-protein-ataxin-3-enables-normal-growth-under-heat-shock-conditions-in-the-methylotrophic-yeast-pichia-pastoris
#7
Marcella Bonanomi, Valentina Roffia, Antonella De Palma, Alessio Lombardi, Francesco Antonio Aprile, Cristina Visentin, Paolo Tortora, Pierluigi Mauri, Maria Elena Regonesi
The protein ataxin-3 carries a polyglutamine stretch close to the C-terminus that triggers a neurodegenerative disease in humans when its length exceeds a critical threshold. A role as a transcriptional regulator but also as a ubiquitin hydrolase has been proposed for this protein. Here, we report that, when expressed in the yeast Pichia pastoris, full-length ataxin-3 enabled almost normal growth at 37 °C, well above the physiological optimum of 30 °C. The N-terminal Josephin domain (JD) was also effective but significantly less, whereas catalytically inactive JD was completely ineffective...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29042626/long-term-cyclability-of-substoichiometric-silicon-nitride-thin-film-anodes-for-li-ion-batteries
#8
Asbjørn Ulvestad, Hanne Flåten Andersen, Jan Petter Mæhlen, Øystein Prytz, Martin Kirkengen
Silicon has been the subject of an extensive research effort aimed at developing new anode materials for lithium ion batteries due to its large specific and volumetric capacity. However, commercial use is limited by a number of degradation problems, many of which are related to the large volume change the material undergoes during cycling in combination with limited lithium-diffusivity. Silicon rich silicon oxides (SiOx), which converts into active silicon and inactive lithium oxide during the initial lithiation, have attracted some attention as a possible solution to these issues...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29041862/impact-of-chronic-maternal-stress-during-early-gestation-on-maternal-fetal-stress-transfer-and-fetal-stress-sensitivity-in-sheep
#9
Michelle Dreiling, Rene Schiffner, Sabine Bischoff, Sven Rupprecht, Nasim Kroegel, Harald Schubert, Otto W Witte, Matthias Schwab, Florian Rakers
Acute stress-induced reduction of uterine blood flow (UBF) is an indirect mechanism of maternal-fetal stress transfer during late gestation. Effects of chronic psychosocial maternal stress (CMS) during early gestation, as may be experienced by many working women, on this stress signaling mechanism are unclear. We hypothesized that CMS in sheep during early gestation augments later acute stress-induced decreases of UBF, and aggravates the fetal hormonal, cardiovascular, and metabolic stress responses during later development...
October 18, 2017: Stress: the International Journal on the Biology of Stress
https://www.readbyqxmd.com/read/29040785/engineering-a-temperature-sensitive-tobacco-etch-virus-protease
#10
J Wong, X Chen, K Truong
Since tobacco etch virus protease (TEVp) has a high specificity and efficiency in cleaving its target substrates, many groups have attempted to engineer conditional control of its activity. Temperature induction is widely used for modulating gene function because it has fast temporal response, good penetrability and applicability to many model organisms. Here, we engineered a temperature sensitive TEVp (tsTEVp) by using N-terminal truncations to TEVp that achieved efficient proteolysis on a timescale of 4 h after 30°C induction, while remaining relatively inactive at 37°C...
October 13, 2017: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/29040440/trends-in-lifestyle-among-three-cohorts-of-adults-aged-55-64-years-in-1992-1993-2002-2003-and-2012-2013
#11
Ilse Reinders, Natasja M van Schoor, Dorly J H Deeg, Martijn Huisman, Marjolein Visser
Background: Unhealthy lifestyle factors, such as obesity, smoking, excessive alcohol consumption and physical inactivity, are associated with increased morbidity and mortality risk, even in older age. We investigated trends in lifestyle among three cohorts of adults aged 55-64 years from the Netherlands. Methods: Data from the Longitudinal Aging Study Amsterdam were used. This study consisted of three randomly selected samples of men and women. Lifestyle data were collected in 1992/1993 (cohort 1, n = 988), in 2002/2003 (cohort 2, n = 1002) and in 2012/2013 (cohort 3, n = 1023)...
October 13, 2017: European Journal of Public Health
https://www.readbyqxmd.com/read/29039977/chronic-disruptions-of-circadian-sleep-regulation-induce-specific-proinflammatory-responses-in-the-rat-colon
#12
Lenka Polidarová, Pavel Houdek, Alena Sumová
Exposure to environmental conditions that disturb the daily rhythms has been shown to enhance the proinflammatory responses of immunostimulant-challenged immune system. However, it is not known whether circadian disturbances may stimulate unchallenged immune responses and thus contribute per se to the development of inflammation-related diseases. Our aim was to ascertain an effect of various conditions threatening the behavioral activity/rest cycle regulation, namely aging with or without melatonin, 6 h advance/delay phase shifts in the light/dark cycle repeated with a 2-day frequency and constant light, on expression of immune markers in the rat colon...
October 17, 2017: Chronobiology International
https://www.readbyqxmd.com/read/29039538/potential-lncrna-diagnostic-biomarkers-for-early-gastric-cancer
#13
Qin Lu, Ting Yu, Xilong Ou, Dazhong Cao, Ting Xie, Xia Chen
Long noncoding RNAs (lncRNAs) serve important functions in many crucial biological processes; however, the effects of lncRNAs in early gastric cancer (EGC) are not entirely clear. The present study aimed to demonstrate the potential of lncRNAs to be used as biomarkers in EGC. Reverse transcription‑quantitative polymerase chain reaction was used to measure the expression levels of lncRNAs, including X inactive‑specific transcript (XIST), Yiya, brain cytoplasmic RNA 1 (BCYRN1), ribosomal RNA processing 1B (RRP1B), KCNQ1 opposite transcript 1 (KCNQ1OT1) and testes development related 1 (TDRG1), in EGC tissues compared with normal adjacent tissues (NATs)...
October 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29039493/actein-induces-apoptosis-in-leukemia-cells-through-suppressing-rhoa-rock1-signaling-pathway
#14
Wen-Di Zhou, Xiang Wang, Xing-Zhen Sun, Jian Hu, Rong-Rong Zhang, Ze Hong
Actein is a tetracyclic triterpenoid compound, extracted from the rhizome of Cimicifuga foetida, exhibiting anticancer activities as previously reported. However, the effects of actein on human leukemia have not been explored before. In this study, the role of actein in regulating apoptosis induction in human leukemia cells was investigated. Actein administration significantly enhanced apoptosis, especially in human leukemia cell line of U937 and the primary human leukemia cells. The promotion was accompanied by caspase-9, caspase-3 and poly(ADP-ribose) polymerase (PARP) cleavage, and cytochrome c (Cyto-c) release...
October 10, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29039348/the-impact-of-agonists-and-antagonists-of-tlr3-and-tlr9-on-concentrations-of-il-6-il10-and-sil-2r-in-culture-supernatants-of-peripheral-blood-mononuclear-cells-derived-from-patients-with-systemic-lupus-erythematosus
#15
Agnieszka Klonowska-Szymczyk, Liliana Kulczycka-Siennicka, Tadeusz Robak, Piotr Smolewski, Barbara Cebula-Obrzut, Ewa Robak
Toll-like receptors (TLR), especially TLR3, 7 and 9, play an important role in the pathogenesis of systemic lupus erythematosus (SLE). In our study blood was collected from 16 patients with SLE and from 8 healthy volunteers. Concentrations of IL-6, IL-10 and sIL-2R were measured by ELISA in mononuclear cell culture supernatant after 24 hours of stimulation by agonists and antagonists of TLR3 and 9 (for TLR3-poli I/C, resveratrol and for TLR9-ODN2006, IRS 945). Stimulation of TLR9 by ODN2006 led to an increase of IL-6 concentration in cell culture supernatants from the cells of healthy volunteers compared with unstimulated cells from controls...
October 13, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/29039281/inhibitory-effects-of-an-extract-from-non-host-plants-on-physiological-characteristics-of-two-major-cabbage-pests
#16
M Dastranj, E Borzoui, A R Bandani, O L Franco
The diamondback moth (Plutella xylostella) and small white cabbage butterfly (Pieris rapae) are the two main serious pests of cruciferous crops (Brassicaceae) that have developed resistance to chemical control methods. In order to avoid such resistance and also the adverse effects of chemical pesticides on the environment, alternative methods have usually been suggested, including the use of plant enzyme inhibitors. Here, the inhibitory effects of proteinaceous inhibitors extracted from wheat, canola, sesame, bean and triticale were evaluated against the digestive α-amylases, larval growth, development and nutritional indecs of the diamondback moth and small white cabbage butterfly...
October 17, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/29039246/regular-practice-of-competitive-sports-does-not-impair-sleep-in-adolescents-dados-study
#17
María Reyes Beltran-Valls, Enrique García Artero, Ana Capdevila-Seder, Alejandro Legaz-Arrese, Mireia Adelantado-Renau, Diego Moliner-Urdiales
PURPOSE: To analyze differences in sleep quality and duration by athletic status and sex, and to examine the association between physical activity (PA) recommendation and sleep in adolescents. METHODS: A total of 267 adolescents [13.9 (0.3) y] from Deporte, ADOlescencia y Salud (DADOS) study (129 girls) were included in this cross-sectional analysis. Athletes competed regularly in organized sport events and trained ≥3 days per week, but nonathletes did not compete...
October 17, 2017: Pediatric Exercise Science
https://www.readbyqxmd.com/read/29039138/the-role-of-gaba-receptor-agonists-in-anesthesia-and-sedation
#18
REVIEW
Janette Brohan, Basavana G Goudra
GABA (γ-aminobutyric acid) receptors, of which there are two types, are involved in inhibitory synapses within the central nervous system. The GABAA receptor (GABAAR) has a central role in modern anesthesia and sedation practice, which is evident from the high proportion of agents that target the GABAAR. Many GABAAR agonists are used in anesthesia practice and sedation, including propofol, etomidate, methohexital, thiopental, isoflurane, sevoflurane, and desflurane. There are advantages and disadvantages to each GABAAR agonist currently in clinical use...
October 16, 2017: CNS Drugs
https://www.readbyqxmd.com/read/29039034/inactivation-of-nsf-atpase-leads-to-cathepsin-b-release-after-transient-cerebral-ischemia
#19
Dong Yuan, Chunli Liu, Bingren Hu
Neurons have extraordinary large cell membrane surface area, thus requiring extremely high levels of intracellular membrane-trafficking activities. Consequently, defects in the membrane-trafficking activities preferentially affect neurons. A critical molecule for controlling the membrane-trafficking activities is the N-ethylmaleimide-sensitive factor (NSF) ATPase. This study is to investigate the cascade of events of NSF ATPase inactivation, resulting in a massive buildup of late endosomes (LEs) and fatal release of cathepsin B (CTSB) after transient cerebral ischemia using the 2-vessel occlusion with hypotension (2VO+Hypotension) global brain ischemia model...
October 17, 2017: Translational Stroke Research
https://www.readbyqxmd.com/read/29038760/cellobiohydrolase-b-of-aspergillus-niger-over-expressed-in-pichia-pastoris-stimulates-hydrolysis-of-oil-palm-empty-fruit-bunches
#20
James Sy-Keen Woon, Mukram M Mackeen, Rosli M Illias, Nor M Mahadi, William J Broughton, Abdul Munir Abdul Murad, Farah Diba Abu Bakar
BACKGROUND: Aspergillus niger, along with many other lignocellulolytic fungi, has been widely used as a commercial workhorse for cellulase production. A fungal cellulase system generally includes three major classes of enzymes i.e., β-glucosidases, endoglucanases and cellobiohydrolases. Cellobiohydrolases (CBH) are vital to the degradation of crystalline cellulose present in lignocellulosic biomass. However, A. niger naturally secretes low levels of CBH. Hence, recombinant production of A...
2017: PeerJ
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