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https://www.readbyqxmd.com/read/29028488/prenatal-phthalate-exposure-and-placental-size-and-shape-at-birth-a-birth-cohort-study
#1
Yuan-Duo Zhu, Hui Gao, Kun Huang, Yun-Wei Zhang, Xiu-Xiu Cai, Hui-Yuan Yao, Lei-Jing Mao, Xing Ge, Shan-Shan Zhou, Yuan-Yuan Xu, Zhong-Xiu Jin, Jie Sheng, Shuang-Qin Yan, Wei-Jun Pan, Jia-Hu Hao, Peng Zhu, Fang-Biao Tao
OBJECTIVE: There is concern over the potential placental effects of prenatal phthalate exposure, and the potential adverse effects of prenatal phthalate exposure require further study; however, few data are available in humans. We investigated the associations between phthalate exposure in each trimester and both placental size and shape at birth. METHODS: We measured the urinary concentrations of phthalate metabolites among 2725 pregnant women in the Ma'anshan Birth Cohort...
October 10, 2017: Environmental Research
https://www.readbyqxmd.com/read/28994558/flexible-co2-laser-fiber-first-look-at-the-learning-curve-required-in-gynecological-laparoscopy-training
#2
Valeria S Vanni, Jessica Ottolina, Giorgio Candotti, Laura M Castellano, Iacopo Tandoi, Francesca DE Stefano, Giorgia Poppi, Stefano Ferrari, Massimo Candiani
BACKGROUND: The advent of flexible CO2 laser fiber to gynecology arena might represent a turning point in the use of laser energy on a large-scale basis in gynecological surgery. However, there might be some concerns regarding the degree of surgical skills required to use the flexible system. The purpose of our study is to evaluate whether flexible CO2 laser fiber is technically accessible. METHODS: Fourteen residents in Obstetrics and Gynecology without surgical experience attending laparoscopic box training with both flexible CO2 laser fiber and traditional line-of-sight CO2 laser using Lumenis AcuPulse Duo CO2 laser (Lumenis, Israel) were prospectively enrolled...
October 9, 2017: Minerva Ginecologica
https://www.readbyqxmd.com/read/28993932/genome-wide-identification-of-cd-responsive-nramp-transporter-genes-and-analyzing-expression-of-nramp-1-mediated-by-mir167-in-brassica-napus
#3
Jin Guo Meng, Xian Duo Zhang, Shang Kun Tan, Kai Xuan Zhao, Zhi Min Yang
In plants, metal transporters are responsible for metal uptake, translocation and homeostasis. These metals include essential nutrients such as zinc (Zn) and manganese (Mn) or non-essential metals like cadmium (Cd) and lead (Pb). Although a few metal transporters have been well characterized in model plants, little is known about their functionality in rapeseed (Brassica napus). In the study, 22 NRAMP transporter genes from B. napus genome were identified and annotated using bioinformatics and high-throughput RNA-sequencing (RNA-seq)...
October 9, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/28967396/effect-of-saliva-contamination-and-artificial-aging-on-different-primer-cement-systems-bonded-to-zirconia
#4
João Pitta, Teresa C Branco, Jaime Portugal
STATEMENT OF PROBLEM: Saliva contamination has been shown to decrease bonding to zirconia. Adopting a less contamination-sensitive cement system may be an alternative to decontamination. PURPOSE: The purpose of this in vitro study was to assess the ability of different primer/cement systems to promote a durable bond to zirconia after saliva contamination. MATERIAL AND METHODS: Zirconia blocks (Lava Plus) (N=320) were airborne-particle abraded (50 μm Al2O3) and divided into 32 experimental groups (n=10) according to the variables in the study: saliva contamination; primer/cement system (Panavia SA [PSA]; RelyX Unicem 2 [RU2]; Bifix SE [BSE]; Panavia F2...
September 27, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28938556/abcg2-and-ncf4-polymorphisms-are-associated-with-clinical-outcomes-in-diffuse-large-b-cell-lymphoma-patients-treated-with-r-chop
#5
Duo Liu, Nan Wu, Haiming Sun, Mei Dong, Tianzhu Guo, Peng Chi, Guofu Li, Donglin Sun, Yan Jin
The impact of pharmacogenetics on predicting survival in diffuse large B-cell lymphoma (DLBCL) remains unclear. We tested 337 DLBCL patients treated with rituximab-cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP) for 9 single nucleotide polymorphisms from 6 genes (CD20, FCGR2A, NAD(P)H, ABCC2, ABCG2 and CYP3A5). Patients who carried the NCF4 rs1883112 GG genotype showed significantly shorter progression-free survival (PFS) (P = 0.023) and event-free survival (EFS) (P < 0.001) comparing with A allele...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28900034/beta-catenin-directs-the-transformation-of-testis-sertoli-cells-to-ovarian-granulosa-like-cells-by-inducing-foxl2-expression
#6
Yaqiong Li, Lianjun Zhang, Yuqiong Hu, Min Chen, Feng Han, Yan Qin, Min Chen, Xiuhong Cui, Shuguang Duo, Fuchou Tang, Fei Gao
Sertoli and granulosa cells are two major types of somatic cells in the male and female gonads,respectively. Previous studies have shown that Sertoli and granulosa cells are derived from common progenitor cells and that differentiation of these two cell types is regulated by sex differentiation genes. The signaling pathway including the adhesion and transcription factor Ctnnb1 (cadherin-associated protein, beta 1, also known as β-catenin) regulates differentiation of granulosa cells in the absence of the transcription factor Sry, and over-activation of β-catenin in the presence of Sry leads to granulosa prior to sex determination...
September 12, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28893550/specificity-of-two-hiv-screening-tests-detecting-simultaneously-hiv-1-p24-antigen-and-antibodies-to-hiv-1-and-2
#7
Annette Blaich, Andreas Buser, Marcel Stöckle, Christian Gehringer, Hans H Hirsch, Manuel Battegay, Thomas Klimkait, Reno Frei
This study aimed at assessing the specificity of the Elecsys(®) HIV combi PT in comparison to the ARCHITECT(®) HIV Ag/Ab Combo. With both of these assays, 3997 unselected sera from patients of a tertiary health care centre in Basel, Switzerland, were screened for HIV. Reactive sera were reanalysed on the VIDAS(®) HIV Duo Ultra to identify false-reactive specimens prior to confirmation by quantitative PCR and line immunoassay. The Elecsys(®) compared to the ARCHITECT(®) shows a similar specificity (99.7% versus 99...
September 8, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28870052/-clinical-efficacy-of-enhanced-recovery-after-surgery-in-atrial-caval-shunting-for-type-%C3%A2-budd-chiari-syndrome
#8
G J Tian, D Y Li, H B Yu, Y D Dong, Y N Peng, P Liu, Y K Wei, H Z Xue
Objective: To investigate the clinical efficacy of enhanced recovery after surgery(ERAS) in atrial caval shunting (ACS) for type Ⅱ Budd-Chiari syndrome(BCS). Methods: The clinical data of patients underwent ACS for type Ⅱ BCS in the Henan Province People's Hospital from January 2014 to June 2016 were prospectively analyzed.Randomized and single-blind, controlled study was performed among the patients, and all of them underwent ACS and were divided into control group (patients underwent traditional perioperative management) and ERAS group (patients underwent ERAS perioperative management) based on a random number table...
September 1, 2017: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
https://www.readbyqxmd.com/read/28868577/x-chromosomal-str-based-genetic-structure-of-sichuan-tibetan-minority-ethnicity-group-and-its-relationships-to-various-groups
#9
Guanglin He, Ye Li, Xing Zou, Ying Zhang, Hepei Li, Mengge Wang, Jin Wu
The X-chromosomal short tandem repeats (STRs) with more informative than autosomal STRs in some complicated biological relationships identification due to its specific mode of genetic transmission can be used as a complementary tool in forensic case practices. In this study, we presented the population genetic data of 19 X-STRs, consisting of DXS10174, DXS10075, DXS10079, DXS101, DXS10101, DXS10103, DXS10134, DXS10135, DXS10148, DXS10159, DXS10162, DXS10164, DXS6789, DXS6809, DXS7132, DXS7423, DXS7424, DXS8378, and HPRTB loci, in a sample of 235 individuals of Tibetan nationality from Sichuan province, Southwest China...
September 3, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28868569/genetic-analysis-of-12-x-str-loci-in-the-serbian-population-from-vojvodina-province
#10
Igor Veselinović, Dušan Vapa, Mihajla Djan, Nevena Veličković, Tanja Veljović, Galina Petrić
The analysis of 12 X-STR loci included in the Investigator® Argus X-12 kit was performed on a sample of 325 unrelated persons from Vojvodina Province, Republic of Serbia. No significant differences were observed in the allele frequencies in males and females. Heterozygosity values among the studied loci ranged from 67.62 to 94.28%. All loci in female individuals were consistent with the Hardy-Weinberg equilibrium test. The combined power of discrimination values in male and female individuals was 0.9999999994 and 0...
September 3, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28835598/influence-of-material-thickness-on-fractural-strength-of-cad-cam-fabricated-ceramic-crowns
#11
Moritz Zimmermann, Gustav Egli, Markus Zaruba, Albert Mehl
The fracture behavior of CAD/CAM fabricated crowns was investigated as a function of material thickness for six silicate ceramic materials: Mark II, e.max CAD, Celtra Duo milled, Celtra Duo fired, Suprinity, Enamic. Crowns with thicknesses 0.5/1.0/1.5 mm were fabricated with CEREC and adhesively seated to dies fabricated with stereolithography technology (n=144). Thermomechanical loading and fractural loading was performed. Statistical analysis was done with one-way ANOVA and post-hoc Scheffé test. For 1.5 mm, all crowns survived fatigue testing, for 1...
August 24, 2017: Dental Materials Journal
https://www.readbyqxmd.com/read/28833798/expressions-of-programmed-death-pd-1-and-pd-1-ligand-pd-l1-in-cervical-intraepithelial-neoplasia-and-cervical-squamous-cell-carcinomas-are-of-prognostic-value-and-associated-with-human-papillomavirus-status
#12
Wen Yang, Yan-Ping Lu, Yi-Zhou Yang, Jia-Rui Kang, Yi-Duo Jin, Hong-Wei Wang
AIM: The programmed death 1/programmed death 1 ligand (PD-1/PD-L1) pathway can decrease the immune clearance effects of antigen-presenting cells and T lymphocytes to promote immune evasion of cervical cancer cells. However, the effects of this pathway on cervical intraepithelial neoplasia (CIN) progression and squamous cell carcinoma (SCC) metastasis are not clear. We herein investigated whether human papillomavirus infection could affect PD-1 and PD-L1 expression in CIN, and whether their expression is associated with CIN progression and SCC metastasis...
August 17, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28831823/mutation-rates-for-20-str-loci-in-a-population-from-s%C3%A3-o-paulo-state-southeast-brazil
#13
Juliana Martinez, Danilo Faustino Braganholi, Isabela Brunelli Ambrósio, Fernanda Silva Polverari, Regina Maria Barretto Cicarelli
Short tandem repeats (STRs) are genetic markers largely employed in forensic analysis and paternity investigation cases. When an inconsistency between the parent and child is considered as a possible mutation, the mutation rate should be incorporated into paternity index calculations to give a robust result and to reduce the chance of misinterpretation. The aim of this study was to estimate the mutation rates of 20 autosomal STRs loci used for paternity tests. In these loci we analysed 29,831 parent-child allelic transfers from 929 duo or trio paternity tests carried out during 2012?2016 from São Paulo State, Brazil...
September 11, 2017: Annals of Human Biology
https://www.readbyqxmd.com/read/28831747/prospective-evaluation-of-the-sd-bioline-dengue-duo-rapid-test-during-a-dengue-virus-epidemic
#14
C Simonnet, A Okandze, S Matheus, F Djossou, M Nacher, A Mahamat
Dengue virus is endemic in French Guiana with occurrence of cyclical outbreaks. There is a need for rapid tests allowing dengue laboratory diagnosis in healthcare centers scattered throughout this wide Amazonian territory. Our objective was to evaluate the real-life performance of the SD BIOLINE Dengue Duo (IgG/IgM + NS1 Ag) rapid test (RDT) during the 2012-2013 dengue epidemics. The RDT was evaluated in parallel with routine laboratory tests, PlateliaTM Dengue NS1 Ag and Focus Diagnostics Dengue Fever Virus IgM Capture DxSelect...
August 22, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28813710/is-endoscopic-vasectomy-just-a-dream-an-ex-vivo-study-on-feasibility-and-reliability-of-endoluminal-occlusion-of-porcine-vas-deferens
#15
Daniel Schlager, Moritz Maas, Simon Hein, Fabian Adams, Martin Schoenthaler, Bjoern Spittau, Ulrich Wetterauer, Arkadiusz Miernik
INTRODUCTION AND AIM: Surgical vasectomy remains the gold standard for fertility control in men. Endoluminal occlusion of the seminal ducts, thus avoiding an external incision, may become an appealing alternative to this approach. As our group has shown, nontraumatic endoscopic inspection of the seminal ducts is feasible in the human cadaver. We investigated the feasibility and reliability of occlusion using several commercially available medical sealing agents in the porcine vas deferens (VD)...
August 17, 2017: Urologia Internationalis
https://www.readbyqxmd.com/read/28752775/significant-genetic-differentiation-of-gobiopterus-lacustris-a-newly-recorded-transparent-goby-in-china
#16
Zhong-Duo Wang, Jian Liao, Cheng-Qin Huang, Shui-Sheng Long, Shun Zhang, Yu-Song Guo, Li Liu, Chu-Wu Liu
In our recent survey, the transparent small Lacustrine goby, Gobiopterus lacustris had reported as the endemic species of Luzon, Philippines, was identified as an abundant species in mangroves of Leizhou Peninsula, China. Here, high diversity and significant differentiation of five sites of samples representing the west and east populations were revealed by mitochondrial DNA sequences. Five haplotypes of 56 cytochrome oxidase subunit I (Cox1) with the lengths of 623 base pairs (bp) have the high pairwise identity (>98...
July 28, 2017: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/28724363/comparing-patient-and-healthcare-worker-experiences-during-a-dengue-outbreak-in-singapore-understanding-the-patient-journey-and-the-introduction-of-a-point-of-care-test-poct-toward-better-care-delivery
#17
Qinghui Tan, Zoe J-L Hildon, Shweta Singh, Jin Jing, Tun Linn Thein, Richard Coker, Hubertus J M Vrijhoef, Yee Sin Leo
BACKGROUND: In the aftermath of an upsurge in the number of dengue cases in 2013 and 2014, the SD BIOLINE Dengue Duo rapid diagnostic Point-of-Care Test (POCT) kit was introduced in Tan Tock Seng Hospital, Singapore in June 2013. It is known that the success of POCT usage is contingent on its implementation within the health system. We evaluated health services delivery and the Dengue Duo rapid diagnostic test kit application in Singapore from healthcare workers' perspectives and patient experiences of dengue at surge times...
July 19, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28720181/computerized-tests-of-team-performance-and-crew-coordination-suitable-for-military-aviation-settings
#18
REVIEW
Ben D Lawson, Thomas W Britt, Amanda M Kelley, Jeremy R Athy, Shauna M Legan
INTRODUCTION: The coordination of team effort on shared tasks is an area of inquiry. A number of tests of team performance in challenging environments have been developed without comparison or standardization. This article provides a systematic review of the most accessible and usable low-to-medium fidelity computerized tests of team performance and determines which are most applicable to military- and aviation-relevant research, such as studies of group command, control, communication, and crew coordination...
August 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28681585/a-simple-method-for-removing-low-density-granulocytes-to-purify-t-lymphocytes-from-peripheral-blood-mononuclear-cells
#19
Si-Gong Zhang, Yu-Xin Song, Xiao-Ming Shu, Hai-Li Shen, Han-Bo Yang, Rui-Xue Duo, Guo-Chun Wang
OBJECTIVE: Low-density granulocytes (LDGs) can form neutrophil extracellular traps (NETs) spontaneously and excessively. When peripheral blood mononuclear cells (PBMCs) are used for studying T lymphocytes, LDGs contained in the PBMCs may decrease the threshold of activating T lymphocytes by forming NETs. This study focused on the profiles of LDGs in common autoimmune diseases and methods for removing LDGs from PBMCs. METHODS: The percentages of LDGs in PBMCs from 55 patients with dermatomyositis (DM), 15 with polymyositis (PM), 42 with rheumatoid arthritis (RA), 25 with systemic lupus erythematosus (SLE), and 19 healthy controls were determined by flow cytometry...
July 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28669717/mirnas-may-regulate-gabaergic-transmission-associated-genes-in-aged-rats-with-anesthetics-induced-recognition-and-working-memory-dysfunction
#20
Ligang Shan, Duo Ma, Chengshen Zhang, Wei Xiong, Yi Zhang
BACKGROUND: Isoflurane and sevoflurane are widely used anesthetics in surgery and administration of these anesthetics could lead to postoperative cognitive dysfunction (POCD). However, the mechanisms remain unclear. METHODS: Aged Wistar rats were exposed to isoflurane and sevoflurane for 2 or 4h. Recognition memory and spatial working memory were measured using Novel object recognition (NOR) and Y-maze test, respectively. Apoptotic cells were detected by TUNEL staining...
September 1, 2017: Brain Research
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