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https://www.readbyqxmd.com/read/28813710/is-endoscopic-vasectomy-just-a-dream-an-ex-vivo-study-on-feasibility-and-reliability-of-endoluminal-occlusion-of-porcine-vas-deferens
#1
Daniel Schlager, Moritz Maas, Simon Hein, Fabian Adams, Martin Schoenthaler, Bjoern Spittau, Ulrich Wetterauer, Arkadiusz Miernik
INTRODUCTION AND AIM: Surgical vasectomy remains the gold standard for fertility control in men. Endoluminal occlusion of the seminal ducts, thus avoiding an external incision, may become an appealing alternative to this approach. As our group has shown, nontraumatic endoscopic inspection of the seminal ducts is feasible in the human cadaver. We investigated the feasibility and reliability of occlusion using several commercially available medical sealing agents in the porcine vas deferens (VD)...
August 17, 2017: Urologia Internationalis
https://www.readbyqxmd.com/read/28752775/significant-genetic-differentiation-of-gobiopterus-lacustris-a-newly-recorded-transparent-goby-in-china
#2
Zhong-Duo Wang, Jian Liao, Cheng-Qin Huang, Shui-Sheng Long, Shun Zhang, Yu-Song Guo, Li Liu, Chu-Wu Liu
In our recent survey, the transparent small Lacustrine goby, Gobiopterus lacustris had reported as the endemic species of Luzon, Philippines, was identified as an abundant species in mangroves of Leizhou Peninsula, China. Here, high diversity and significant differentiation of five sites of samples representing the west and east populations were revealed by mitochondrial DNA sequences. Five haplotypes of 56 cytochrome oxidase subunit I (Cox1) with the lengths of 623 base pairs (bp) have the high pairwise identity (>98...
July 28, 2017: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/28724363/comparing-patient-and-healthcare-worker-experiences-during-a-dengue-outbreak-in-singapore-understanding-the-patient-journey-and-the-introduction-of-a-point-of-care-test-poct-toward-better-care-delivery
#3
Qinghui Tan, Zoe J-L Hildon, Shweta Singh, Jin Jing, Tun Linn Thein, Richard Coker, Hubertus J M Vrijhoef, Yee Sin Leo
BACKGROUND: In the aftermath of an upsurge in the number of dengue cases in 2013 and 2014, the SD BIOLINE Dengue Duo rapid diagnostic Point-of-Care Test (POCT) kit was introduced in Tan Tock Seng Hospital, Singapore in June 2013. It is known that the success of POCT usage is contingent on its implementation within the health system. We evaluated health services delivery and the Dengue Duo rapid diagnostic test kit application in Singapore from healthcare workers' perspectives and patient experiences of dengue at surge times...
July 19, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28720181/computerized-tests-of-team-performance-and-crew-coordination-suitable-for-military-aviation-settings
#4
Ben D Lawson, Thomas W Britt, Amanda M Kelley, Jeremy R Athy, Shauna M Legan
INTRODUCTION: The coordination of team effort on shared tasks is an area of inquiry. A number of tests of team performance in challenging environments have been developed without comparison or standardization. This article provides a systematic review of the most accessible and usable low-to-medium fidelity computerized tests of team performance and determines which are most applicable to military- and aviation-relevant research, such as studies of group command, control, communication, and crew coordination...
August 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28681585/a-simple-method-for-removing-low-density-granulocytes-to-purify-t-lymphocytes-from-peripheral-blood-mononuclear-cells
#5
Si-Gong Zhang, Yu-Xin Song, Xiao-Ming Shu, Hai-Li Shen, Han-Bo Yang, Rui-Xue Duo, Guo-Chun Wang
OBJECTIVE: Low-density granulocytes (LDGs) can form neutrophil extracellular traps (NETs) spontaneously and excessively. When peripheral blood mononuclear cells (PBMCs) are used for studying T lymphocytes, LDGs contained in the PBMCs may decrease the threshold of activating T lymphocytes by forming NETs. This study focused on the profiles of LDGs in common autoimmune diseases and methods for removing LDGs from PBMCs. METHODS: The percentages of LDGs in PBMCs from 55 patients with dermatomyositis (DM), 15 with polymyositis (PM), 42 with rheumatoid arthritis (RA), 25 with systemic lupus erythematosus (SLE), and 19 healthy controls were determined by flow cytometry...
July 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28669717/mirnas-may-regulate-gabaergic-transmission-associated-genes-in-aged-rats-with-anesthetics-induced-recognition-and-working-memory-dysfunction
#6
Ligang Shan, Duo Ma, Chengshen Zhang, Wei Xiong, Yi Zhang
BACKGROUND: Isoflurane and sevoflurane are widely used anesthetics in surgery and administration of these anesthetics could lead to postoperative cognitive dysfunction (POCD). However, the mechanisms remain unclear. METHODS: Aged Wistar rats were exposed to isoflurane and sevoflurane for 2 or 4h. Recognition memory and spatial working memory were measured using Novel object recognition (NOR) and Y-maze test, respectively. Apoptotic cells were detected by TUNEL staining...
September 1, 2017: Brain Research
https://www.readbyqxmd.com/read/28664332/heparin-gold-nanoparticles-for-enhanced-microdialysis-sampling
#7
Susan Giorgi-Coll, Holly Blunt-Foley, Peter J Hutchinson, Keri L H Carpenter
Cerebral microdialysis is a sampling technique which offers much potential for understanding inflammatory pathophysiology following traumatic brain injury (TBI). At present, the recovery of cytokines via microdialysis in clinical studies is not straightforward primarily due to their size, steric properties and low concentrations. Heparin and heparin-coated microspheres have previously shown promise as cytokine-binding agents for enhanced microdialysis sampling in animal models (Duo and Stenken in Anal Bioanal Chem 399(2):773-82, 2011; Anal Bioanal Chem 399(2):783-93, 2011)...
August 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28661234/an-evaluation-of-the-sd-bioline-hiv-syphilis-duo-test
#8
Jeffrey Holden, Joshua Goheen, Mary Jett-Goheen, Mathilda Barnes, Yu-Hsiang Hsieh, Charlotte A Gaydos
Many health agencies now recommend routine HIV and syphilis testing for pregnant women and most-at-risk populations such as men who have sex with men. With the increased availability of highly sensitive, low cost rapid point-of-care tests, the ability to meet those recommendations has increased, granting wider access to quick and accurate diagnoses. Using blood specimens collected from a Baltimore City Health Department (BCHD) sexually transmitted infection clinic, we evaluated the SD Bioline HIV/Syphilis Duo, a rapid test that simultaneously detects antibodies to HIV and syphilis and has the potential to further benefit clinics and patients by reducing costs, testing complexity, and patient wait times...
January 1, 2017: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/28642930/a-novel-etching-technique-for-surface-treatment-of-zirconia-ceramics-to-improve-adhesion-of-resin-based-luting-cements
#9
Eystein Ivar Ruyter, Nalini Vajeeston, Torbjørn Knarvang, Ketil Kvam
Objectives: Bonding of zirconia crowns and bridges to abutments is important, not only bonding of the thin resin layer to the abutment, but also bonding to the zirconia ceramic is crucial. Both mechanical and chemical adhesion are desired. Mechanical retention of dental porcelain achieved by etching with moderately concentrated hydrofluoric acid is not possible with zirconia ceramics. The purpose of this study was to show that etching is possible with relative low melting fluoride compounds such as ammonium hydrogen difluoride and potassium hydrogen difluoride...
January 2017: Acta Biomaterialia Odontologica Scandinavica
https://www.readbyqxmd.com/read/28642922/universal-cements-dual-activated-and-chemically-activated
#10
Eliane de Lima, Ricardo Santos, Márcia Durão, Armiliana Nascimento, Rodivan Braz
Objective: The aim of the present study was to assess the bond strength of universal cements cured either dually or chemically only. Methods: Three cements were assessed using different types of application: dual activated (DA) or chemically activated (CA). In total 80 dentin blocks were used, obtained through the enamel wear of the lingual and buccal surfaces of bovine incisors. Standard cone-shaped cavity preparations were created using diamond burs. Subsequently, indirect restoration blocks were designed with Filtek Z350 (3M ESPE) composite resin...
December 2016: Acta Biomaterialia Odontologica Scandinavica
https://www.readbyqxmd.com/read/28619082/assessing-changes-in-knowledge-attitude-and-practices-on-dengue-diagnosis-and-management-among-primary-care-physicians-after-the-largest-dengue-epidemic-in-singapore
#11
Junxiong Pang, Zoe Jane-Lara Hildon, Tun Linn Thein, Jing Jin, Yee Sin Leo
BACKGROUND: Dengue results in high morbidity and mortality globally. The knowledge, attitude and practices (KAP) of dengue management, including diagnosis, among primary care physicians (PCPs) are important to reduce dengue transmission and burden. However, there is a lack of understanding on the impact of dengue epidemic on dengue management. Hence, the aim of this study is to examine the changes in KAP on dengue management among PCPs before and after the largest dengue epidemic in 2013 in Singapore...
June 15, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28606319/seroprevalence-of-and-risk-factors-for-neospora-caninum-infection-in-yaks-bos-grunniens-in-china
#12
Qing-Feng Meng, Gui-Zhe Yao, Si-Yuan Qin, Jian Wu, Xi-Chen Zhang, Ya-Duo Bai, Wei Cong, Wei-Li Wang
The aim of the present study was to detect the seroprevalence of Neospora caninum infection in yaks in Gansu province, China. Serum samples from 974 white yaks and 610 black yaks were tested by a commercial competitive-inhibition ELISA for the detection of specific anti-N. caninum antibodies.; N. caninum antibodies were detected in 10.4% of yaks with higher prevalence in black (11.5% of 610) than white yak (8.6% of 974). Age, regions and sampling times were considered as risk factors associated with N. caninum infection...
August 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28604032/gaze-behavior-to-faces-during-dyadic-interaction
#13
Roy S Hessels, Tim H W Cornelissen, Ignace T C Hooge, Chantal Kemner
A long-standing hypothesis is that humans have a bias for fixating the eye region in the faces of others. Most studies have tested this hypothesis with static images or videos of faces, yet recent studies suggest that the use of such "nonresponsive" stimuli might overlook an influence of social context. The present study addressed whether the bias for fixating the eye region in faces would persist in a situation that allowed for social interaction. In Experiment 1, we demonstrate a setup in which a duo could engage in social interaction while their eye movements were recorded...
June 12, 2017: Canadian Journal of Experimental Psychology, Revue Canadienne de Psychologie Expérimentale
https://www.readbyqxmd.com/read/28600779/the-landscape-of-genetic-diseases-in-saudi-arabia-based-on-the-first-1000-diagnostic-panels-and-exomes
#14
Dorota Monies, Mohamed Abouelhoda, Moeenaldeen AlSayed, Zuhair Alhassnan, Maha Alotaibi, Husam Kayyali, Mohammed Al-Owain, Ayaz Shah, Zuhair Rahbeeni, Mohammad A Al-Muhaizea, Hamad I Alzaidan, Edward Cupler, Saeed Bohlega, Eissa Faqeih, Maha Faden, Banan Alyounes, Dyala Jaroudi, Ewa Goljan, Hadeel Elbardisy, Asma Akilan, Renad Albar, Hesham Aldhalaan, Shamshad Gulab, Aziza Chedrawi, Bandar K Al Saud, Wesam Kurdi, Nawal Makhseed, Tahani Alqasim, Heba Y El Khashab, Hamoud Al-Mousa, Amal Alhashem, Imaduddin Kanaan, Talal Algoufi, Khalid Alsaleem, Talal A Basha, Fathiya Al-Murshedi, Sameena Khan, Adila Al-Kindy, Maha Alnemer, Sami Al-Hajjar, Suad Alyamani, Hasan Aldhekri, Ali Al-Mehaidib, Rand Arnaout, Omar Dabbagh, Mohammad Shagrani, Dieter Broering, Maha Tulbah, Amal Alqassmi, Maisoon Almugbel, Mohammed AlQuaiz, Abdulaziz Alsaman, Khalid Al-Thihli, Raashda A Sulaiman, Wajeeh Al-Dekhail, Abeer Alsaegh, Fahad A Bashiri, Alya Qari, Suzan Alhomadi, Hisham Alkuraya, Mohammed Alsebayel, Muddathir H Hamad, Laszlo Szonyi, Faisal Abaalkhail, Sulaiman M Al-Mayouf, Hamad Almojalli, Khalid S Alqadi, Hussien Elsiesy, Taghreed M Shuaib, Mohammed Zain Seidahmed, Ibraheem Abosoudah, Hana Akleh, Abdulaziz AlGhonaium, Turki M Alkharfy, Fuad Al Mutairi, Wafa Eyaid, Abdullah Alshanbary, Farrukh R Sheikh, Fahad I Alsohaibani, Abdullah Alsonbul, Saeed Al Tala, Soher Balkhy, Randa Bassiouni, Ahmed S Alenizi, Maged H Hussein, Saeed Hassan, Mohamed Khalil, Brahim Tabarki, Saad Alshahwan, Amira Oshi, Yasser Sabr, Saad Alsaadoun, Mustafa A Salih, Sarar Mohamed, Habiba Sultana, Abdullah Tamim, Moayad El-Haj, Saif Alshahrani, Dalal K Bubshait, Majid Alfadhel, Tariq Faquih, Mohamed El-Kalioby, Shazia Subhani, Zeeshan Shah, Nabil Moghrabi, Brian F Meyer, Fowzan S Alkuraya
In this study, we report the experience of the only reference clinical next-generation sequencing lab in Saudi Arabia with the first 1000 families who span a wide-range of suspected Mendelian phenotypes. A total of 1019 tests were performed in the period of March 2016-December 2016 comprising 972 solo (index only), 14 duo (parents or affected siblings only), and 33 trio (index and parents). Multigene panels accounted for 672 tests, while whole exome sequencing (WES) represented the remaining 347 tests. Pathogenic or likely pathogenic variants that explain the clinical indications were identified in 34% (27% in panels and 43% in exomes), spanning 279 genes and including 165 novel variants...
August 2017: Human Genetics
https://www.readbyqxmd.com/read/28570875/the-impact-of-modified-nano-carbon-black-on-the-earthworm-eisenia-fetida-under-turfgrass-growing-conditions-assessment-of-survival-biomass-and-antioxidant-enzymatic-activities
#15
Shulan Zhao, Lu He, Yunfeng Lu, Lian Duo
Modified nano-carbon adsorbents have been employed in the immobilization of heavy metals in soil due to their good adsorption capabilities regarding metal ions. However, an assessment of their risks has not been extensively performed with soil organisms. To assess the toxic effects of three types of modified nano-carbon black (CB) on soil organisms, a laboratory test was conducted to expose the earthworm Eisenia fetida to artificial soil supplemented with 5% H2SO4-, HNO3- and KMnO4-modified nano-CB (SCB, NCB and KCB, respectively) under turfgrass growing conditions...
May 22, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28544495/posterior-lumbar-interbody-fusion-with-interspinous-fastener-provides-comparable-clinical-outcome-and-fusion-rate-to-pedicle-screws
#16
Wei-Min Huang, Xing-Ming Yu, Xiao-Duo Xu, Ruo-Xian Song, Li-Li Yu, Xiu-Chun Yu
OBJECTIVE: To investigate the clinical feasibility and validity of interspinous fastener (ISF) for lumbar degenerative diseases. METHODS: From October 2013 to March 2014, a total of 46 patients suffering from lumbar degenerative diseases underwent posterior lumbar interbody fusion (PLIF) randomly augmented by ISF or pedicle screws. The clinical outcome was primarily measured by Oswestry Disability Index (ODI) score. The minimal clinical important difference (MCID) was defined as an eight-point decrease in ODI...
May 24, 2017: Orthopaedic Surgery
https://www.readbyqxmd.com/read/28522589/identification-of-cancer-targeted-tropomyosin-inhibitors-and-their-synergy-with-microtubule-drugs
#17
Mark A Currier, Justine R Stehn, Ashleigh Swain, Duo Chen, Jeff Hook, Eleanor Eiffe, Andrew Heaton, David Brown, Brooke Nartker, David W Eaves, Nina Kloss, Herbert Treutlein, Jun Zeng, Irina B Alieva, Vera B Dugina, Edna C Hardeman, Peter W Gunning, Timothy P Cripe
Actin filaments, with their associated tropomyosin polymers, and microtubules are dynamic cytoskeletal systems regulating numerous cell functions. While anti-microtubule drugs are well-established, anti-actin drugs have been more elusive. We previously targeted actin in cancer cells by inhibiting the function of a tropomyosin isoform enriched in cancer cells, Tpm3.1, using a first-in-class compound, TR100. Here, we screened over 200 other anti-tropomyosin analogues for anti-cancer and on-target activity using a series of in vitro cell-based and biochemical assays...
May 18, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/28473770/pharmacokinetic-properties-of-three-forms-of-vaginal-progesterone-administered-in-either-single-or-multiple-dose-regimen-in-healthy-post-menopausal-chinese-women
#18
Guolan Wu, Junchun Chen, Xingjiang Hu, Huili Zhou, Jian Liu, Duo Lv, Lihua Wu, Jianzhong Shentu
Objective: A generic vaginal progesterone gel has recently been developed in China. Little is known about its pharmacokinetic properties in Chinese subjects. The purpose of our study was to investigate the pharmacokinetics of three forms of vaginal progesterone gel (test formulations at 4 and 8% strength vs. a reference formulation: Crinone 8%) in Chinese healthy post-menopausal women. Methods: This study consisted of two parts study. The part 1 study was a single-center, open-label, 3-period study. Twelve healthy post-menopausal women were to evaluate the safety and pharmacokinetics of 45 mg vaginal progesterone gel (Test 4%) following single dose and multiple doses administered once every other day (q...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28460938/idiographic-duo-trio-tests-using-a-constant-reference-based-on-preference-of-each-consumer-sample-presentation-sequence-in-difference-test-can-be-customized-for-individual-consumers-to-reduce-error
#19
Min-A Kim, Hye-Min Sim, Hye-Seong Lee
As reformulations and processing changes are increasingly needed in the food industry to produce healthier, more sustainable, and cost effective products while maintaining superior quality, reliable measurements of consumers' sensory perception and discrimination are becoming more critical. Consumer discrimination methods using a preferred-reference duo-trio test design have been shown to be effective in improving the discrimination performance by customizing sample presentation sequences. However, this design can add complexity to the discrimination task for some consumers, resulting in more errors in sensory discrimination...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28452995/twelve-year-analysis-of-microbial-keratitis-trends-at-a-uk-tertiary-hospital
#20
S Z Tan, A Walkden, L Au, C Fullwood, A Hamilton, A Qamruddin, M Armstrong, A K Brahma, F Carley
PurposeTo investigate the frequencies, trends, and in vitro drug susceptibilities of the causative pathogens in microbial keratitis in Manchester Royal Eye Hospital.Patients and methodsCorneal scrape results recorded by the microbiology service between 2004 and 2015 were extracted from an established database. A total of 4229 corneal scrape specimens were identified from an established database. First-line antibiotic treatment in our centre during the study period was ofloxacin and second line was cefuroxime and gentamicin...
April 28, 2017: Eye
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