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Duo test

Bingbing Wu, Duo Pan, Xiaoyan Zheng, Haichen Ding, Zhenlong Ma, Meina Xie, Shan Ge
OBJECTIVE: To investigate the long-term effects and the mechanism of neonatal bisphenol A( BPA) exposure on mouse testicular structure and Boule expression. METHODS: A total of 12 pregnant ICR mice were randomly divided into three groups:blank control group, negative control group( corn oil) and BPA 100 μg/kg group. After delivery, BPA was given daily by neck subcutaneous injection to the offspring male mice from postnatal day( PND 1) to PND 21. The offspring male mice were sacrificed on PND35 and PND 70...
November 2017: Wei Sheng Yan Jiu, Journal of Hygiene Research
Keming Mao, Duo Lu, Dazhi E, Zhenhua Tan
Heated metal mark is an important trace to identify the cause of fire. However, traditional methods mainly focus on the knowledge of physics and chemistry for qualitative analysis and make it still a challenging problem. This paper presents a case study on attribute recognition of the heated metal mark image using computer vision and machine learning technologies. The proposed work is composed of three parts. Material is first generated. According to national standards, actual needs and feasibility, seven attributes are selected for research...
June 7, 2018: Sensors
Mélissa Julien, Laury N'guema, Amina Bouzerara, Benoit Toro, Edouard Lecarpentier, Jean Guibourdenche
Premature rupture of the membranes (PROM) is a frequent event affecting 3% of pregnancies. PROM causes 30% of premature deliveries and 20% of perinatal mortality. The diagnosis relies mainly on the clinical visualization of the amniotic fluid flow in the vagina. If not, clinicians can use bedside tests detecting either the change of the vaginal pH or the presence of amniotic components mainly IGFBP-1 or AFP in the vaginal fluid. We aimed to study the technical and analytical characteristics of 5 immunochromatographic tests (easyProm®, ActimProm®, Toda Amniodiag 5 strip®, Amnioquick® Duo, Amnisure®) that mainly detect IGFBP-1 in order to compare our results with the data from the manufacturer...
June 1, 2018: Annales de Biologie Clinique
Fereshteh Shafiei, Maryam Saadat, Zahra Jowkar
Background: This study evaluated the effect of three different silanes and post-silanization treatments on the retentive strength of fiber posts luted with an etch-and-rinse resin cement. Material and Methods: One hundred intact maxillary central incisors were randomly divided into 10 groups after endodontic treatment and post space preparation (n=10). The fiber posts were etched using 24% hydrogen peroxide. Posts of the control group did not receive silane. In nine experimental groups, each of the three silanes used, Scotchbond Universal adhesive, Bis-Silane and Porcelain Primer, was subjected to three treatments: air-drying at 25°C, warm air-drying and CO2 laser heat treatment...
May 2018: Journal of Clinical and Experimental Dentistry
Chuanfu Dai, Zeqi Zhao, Weidong Shen, Duo Zhang, Guanxiong Lei, Yuehua Qiao, Shiming Yang
BACKGROUND The aim of this study was to explore the value of the spectral ripple discrimination test in speech recognition evaluation among a deaf (post-lingual) Mandarin-speaking population in China following cochlear implantation. MATERIAL AND METHODS The study included 23 Mandarin-speaking adult subjects with normal hearing (normal-hearing group) and 17 deaf adults who were former Mandarin-speakers, with cochlear implants (cochlear implantation group). The normal-hearing subjects were divided into men (n=10) and women (n=13)...
May 28, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Man Chen, Jian Jiang, Chen Li, He Ren, Wei Chen, Zhiyong Liu, Feng Cheng, Jing Zhao, Tong Chen, Chuguang Chen, Jiangwei Yan
We present a duo paternity test case to assess the biological relationship between a woman and her female child. After analyzing 57 autosomal and 19 X-chromosomal short tandem repeat loci, mother-daughter exclusions were discovered at four loci, which were all located on chromosome 2. Further testing of whole-genome single nucleotide polymorphisms confirmed that the daughter had complete uniparental disomy (UPD) of chromosome 2. This study presents a cautionary case demonstrating that hasty decisions of parentage exclusion should not be made when genetic markers on the same chromosome do not conform to Mendel's laws due to UPD...
May 25, 2018: International Journal of Legal Medicine
Yi Wu, Rui-Ying Zhu, Leslie A Mitchell, Lu Ma, Rui Liu, Meng Zhao, Bin Jia, Hui Xu, Yun-Xiang Li, Zu-Ming Yang, Yuan Ma, Xia Li, Hong Liu, Duo Liu, Wen-Hai Xiao, Xiao Zhou, Bing-Zhi Li, Ying-Jin Yuan, Jef D Boeke
The power of synthetic biology has enabled the expression of heterologous pathways in cells, as well as genome-scale synthesis projects. The complexity of biological networks makes rational de novo design a grand challenge. Introducing features that confer genetic flexibility is a powerful strategy for downstream engineering. Here we develop an in vitro method of DNA library construction based on structural variation to accomplish this goal. The "in vitro SCRaMbLE system" uses Cre recombinase mixed in a test tube with purified DNA encoding multiple loxPsym sites...
May 22, 2018: Nature Communications
Bo Cao, Ying Zhang, Ziyi Wang, Mengyuan Li, Feng Yang, Duo Jiang, Zhao Jiang
Although plants of the genus Pennisetum can accelerate the removal of atrazine from its rhizosphere, the roles played by this plant in adjusting the soil environment and soil microorganism properties that might contribute to pollutant removal are incompletely understood. We selected Pennisetum americanum (L.) K. Schum ( P. americanum ) as the test plant and investigated the interaction between P. americanum and atrazine-contaminated soil, focusing on the adjustment of the soil biochemical properties as well as bacterial functional and community diversity in the rhizosphere using Biolog EcoPlates and high-throughput sequencing of the 16S rRNA gene...
2018: Frontiers in Microbiology
Dong-Qiong Yang, Zhao-Rong Chen, Duo-Zhi Chen, Xiao-Jiang Hao, Shun-Lin Li
Fifteen known amaryllidaceae alkaloids were isolated from the bulbs of Lycoris radiata. Some of the compounds and lycoricidine derivatives had been screened for the activities against tobacco mosaic virus (TMV) by the conventional half-leaf method. Lycoricidine derivatives were also carried out the assay of effect on systemic infection of TMV by western-blot and RT-PCR analysis. The tested compounds showed moderate inactivation effect, whereas the lycoricidine derivatives showed good protective effect. The protective inhibitory activity of compounds L1 (N-methyl-2,3,4-trimethoxylycoricidine) (60...
May 18, 2018: Natural Products and Bioprospecting
Mike Matzinger, Sebastian Hahnel, Verena Preis, Martin Rosentritt
OBJECTIVES: To investigate the surface roughness of CAD/CAM materials immediately after milling and after different chairside and labside polishing procedures. A two-body wear test was performed to compare the different wear characteristics of the materials and the corresponding antagonists. MATERIALS AND METHODS: Specimens (n = 12 per series) from different CAD/CAM materials (three composites: Lava Ultimate, Cerasmart, BRILLIANT Crios; one hybrid ceramic: VITA Enamic; three ceramics: Celtra Duo, VITA Suprinity, IPS Emax...
May 16, 2018: Clinical Oral Investigations
Taiguo Liu, Lina Duo, Ping Duan
Background: Radiation therapy is an important mode of colorectal cancer treatment. However, most people die of local recurrence after tumors become resistant to radiotherapy, and little progress has been made in treating radiotherapy-resistant colorectal cancer. Hence, novel agents that are nontoxic and can sensitize colorectal cancer to radiotherapy are urgently needed. Ginsenoside Rg3, a saponin extracted from ginseng, shows cytotoxicity against a variety of cancer cells through suppression of pathways linked to oncogenesis, including cell survival, proliferation, invasion, and angiogenesis...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Orysya O Syzon, Marianna O Dashko, Ulyana V Fedorova
OBJECTIVE: Introduction:Psoriasis affects about 2% of population. In 30-40% of occurrences arthropathic psoriasis (AP) is diagnosed and it leads to 11-19% of disability cases development. Recent studies have shown that psoriasis is often synergistically combined with herpesvirus of the 1st and 2nd type, which, according to many scientists, determines the severity, frequency of recurrences, and the course of this dermatosis. Recently, new biomarkers, i.e. a system of small ribonucleic microRNA acids, have been described...
2018: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
Luiz Carlos do Carmo Filho, Fernanda Faot, Marina de Matos Madruga, Raissa Micaella Marcello-Machado, Dimorvan Bordin, Altair Antoninha Del Bel Cury
OBJECTIVES: This randomized split-mouth clinical trial investigated the influence of implant macrogeometry on bone properties and peri-implant health parameters during the healing process. MATERIAL AND METHODS: Ninety-nine implants were placed bilaterally in posterior mandibles of 23 patients that received at least four dental implant macrogeometries: standard geometry, Integra (IN) and three geometries inducing "healing chamber": Duo (D), Compact (C), and Infra (IF)...
May 3, 2018: Clinical Oral Investigations
Omri Weiss, Anat Levi, Elad Segev, Margarita Simbirsky, David Eilam
In the present study, the dynamic process of group formation in eight unfamiliar rats was followed in order to reveal how the group becomes oriented together in time and space, in light of the complexity that accompanies grouping. The focus was on who, where, and when joined together. We found that rats preferred to be in companionship over remaining alone, with all the rats gradually shifting to share the same location as a resting place. Group formation can be viewed as a tri-phasic process, with some rats gradually becoming more social than others, and thus playing a key role in group formation...
July 2018: Animal Cognition
Fairuz Amran, Yii Ling Liow, Nurul Atiqah Noor Halim
Leptospirosis is a febrile zoonotic disease. Routine diagnosis of leptospirosis is based on the detection of specific antibodies with serological tests. The aim of our study was to determine the usefulness of immunochromatographic assay (ICA), ImmuneMed Leptospira IgM Duo Rapid test kit from Korea, in rapid screening of acute leptospirosis in emergency cases with limited expertise. A total of 197 serum samples (93 positive, 104 negative) were selected randomly. The test has good diagnostic sensitivity 73% and specificity 90%...
April 23, 2018: Journal of Korean Medical Science
Michael Wendler, Renan Belli, Diana Valladares, Anselm Petschelt, Ulrich Lohbauer
OBJECTIVES: Chemical and mechanical degradation play a key role on the lifetime of dental restorative materials. Therefore, prediction of their long-term performance in the oral environment should base on fatigue, rather than inert strength data, as commonly observed in the dental material's field. The objective of the present study was to provide mechanistic fatigue parameters of current dental CAD/CAM materials under cyclic biaxial flexure and assess their suitability in predicting clinical fracture behaviors...
June 2018: Dental Materials: Official Publication of the Academy of Dental Materials
R Grasel, M J Santos, Hm Chagas Rêgo, M P Rippe, L F Valandro
This study aimed to evaluate the effect of different primer/resin luting agent combinations and alumina air abrasion on the adhesion to zirconia. Eighty blocks (4×4×3 mm) of Lava Frame Zirconia (3M ESPE) were produced and randomly assigned into eight groups (n=10) according to two zirconia surface treatments (untreated or air abrasion with 50-μm alumina particles) and four luting systems (SU: Scotchbond Universal/RelyX Unicem 2; ZP: Z-Prime Plus/Duo-link Universal; MB: Monobond Plus/Variolink II; and AP: Alloy Primer/ED Primer II/Panavia F 2...
May 2018: Operative Dentistry
Die Zhang, Wei Zhou, Thanh Thai Lam, Connie Weng, Lawrence Bronk, Duo Ma, Qiang Wang, Joseph G Duman, Patrick M Dougherty, David R Grosshans
Background: Radiation-induced cognitive dysfunction is a significant side effect of cranial irradiation for brain tumors. Clinically, pediatric patients are more vulnerable than adults. However, the underlying mechanisms of dysfunction, including reasons for age dependence, are still largely unknown. Previous studies have focused on the loss of hippocampal neuronal precursor cells and deficits in memory. However, survivors may also experience deficits in attention, executive function, or other non-hippocampal-dependent cognitive domains...
April 6, 2018: Neuro-oncology
S L Zhao, F Zhao, Y G Sha, Q X Chen, X Q Cheng, S M Huang
Objective: To summarize the clinical features and genetic analysis results of 10 children with Dent disease. Methods: The clinical data and gene test results of 10 boys aged from 8 months to 12 years with Dent disease diagnosed in Children's Hospital of Nanjing Medical University from January 2014 to July 2017 were analyzed retrospectively. Results: All patients had insidious onset, 5 cases were found to have proteinuria on routine urine examination after hospitalization duo to other diseases, 4 cases were admitted to hospital because increased foams in the urine, and 1 case was found to have proteinuria on health checkup...
April 2, 2018: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
Shunli Ji, Tengfei Li, Wen Yang, Chang Shu, Duo Li, Yan Wang, Li Ding
A hollow porous molecularly imprinted polymer (HPMIP) is described for use in dispersive solid phase extraction of macrolide antibiotics (MACs). The HPMIP was prepared by using spiramycin as the template, methacrylic acid as the functional monomer, and mesoporous MCM-41 (Mobile Composition of Matter No. 41; with a size of about 100 nm) as a sacrificial support. The sorbent was characterized by Fourier transform infrared spectrometry, transmission electron microscopy, nitrogen adsorption and thermo-gravimetric analysis...
March 1, 2018: Mikrochimica Acta
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