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https://www.readbyqxmd.com/read/29441768/-application-of-snp-str-composed-by-d18s51-and-three-snps-of-its-flanking-region-in-paternity-testing
#1
J S Gao, Y Ye, Y P Hou
OBJECTIVES: To develop a SNP-STR haplotype by consisting of the SNP and STR genetic markers, both of which locate in a haplotype block. To investigate its distribution in Han population from Chengdu, and explore its application in some special cases of paternity testing. METHODS: D18S51 , one of the high mutation rate STR markers in combined DNA index system (CODIS), and three SNP loci rs8089331, rs8094489 and rs7236090 in its flanking region, were chosen to establish SNP-STR...
December 2017: Fa Yi Xue za Zhi
https://www.readbyqxmd.com/read/29430171/correlation-between-hla-haplotypes-and-the-development-of-antidrug-antibodies-in-a-cohort-of-patients-with-rheumatic-diseases
#2
Maurizio Benucci, Arianna Damiani, Francesca Li Gobbi, Francesca Bandinelli, Maria Infantino, Valentina Grossi, Mariangela Manfredi, Guillaume Noguier, Francesca Meacci
Introduction: The aim of this study was to investigate the correlation between human leukocyte antigen (HLA) haplotypes and the development of antidrug antibodies (ADAs) in a cohort of patients with rheumatic diseases. Patients and methods: We evaluated the presence of ADAs in 248 patients with inflammatory rheumatic diseases after 6 months of treatment with anti-TNF drugs: 26 patients were treated with infliximab (IFX; three with rheumatoid arthritis [RA], 13 with ankylosing spondylitis [AS], 10 with psoriatic arthritis [PsA]); 83 treated with adalimumab (ADA; 24 with RA, 36 with AS, 23 with PsA); 88 treated with etanercept (ETA; 35 with RA, 27 with AS, 26 with PsA); 32 treated with certolizumab (CERT; 25 with RA, two with AS, five with PsA); and 19 treated with golimumab (GOL; three with RA, seven with AS, nine with PsA)...
2018: Biologics: Targets & Therapy
https://www.readbyqxmd.com/read/29404810/roughness-surface-energy-and-superficial-damages-of-cad-cam-materials-after-surface-treatment
#3
Thomas Strasser, Verena Preis, Michael Behr, Martin Rosentritt
OBJECTIVES: The aim of this study was to examine the effects of surface pre-treatment on CAD/CAM materials including ceramics, zirconia, resin-infiltrated ceramic, and resin-based composite. MATERIALS AND METHODS: Specimens were made of ten CAD/CAM materials (Celtra Duo, Degudent, D; Vita Suprinity, Vita, D; E.max CAD, Ivoclar-Vivadent, FL; E.max ZirCAD, Ivoclar-Vivadent, FL; Vita Enamic, Vita, D; Cerasmart, GC, B; LAVA Ultimate, 3M, D; SHOFU Block HC, SHOFU, US; Grandio Blocs, VOCO, D; BRILLIANT Crios, Coltene, CH) and pretreated to represent clinical procedures (Hf 20 s/5%; phosphoric acid 20 s/37%; Monobond etch and prime (Ivoclar-Vivadent, FL); water-cooled diamond bur (80 μm; 4 μm); Al2O3-blasting (50 μm/1 bar, 50 μm/2 bar, 120 μm/1 bar, 120 μm/2 bar); untreated; manufacturer's instructions)...
February 5, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29376482/effects-of-orthopedic-insoles-on-static-balance-of-older-adults-wearing-thick-socks
#4
Christina Zong-Hao Ma, Duo Wai-Chi Wong, Anson Hong-Ping Wan, Winson Chiu-Chun Lee
BACKGROUND: The wearing of socks and insoles may affect the ability of the foot to detect tactile input influencing postural balance. OBJECTIVES: The aim of this study was to investigate whether (1) thick socks adversely affected the elderly postural balance and (2) orthopedic insoles could improve the elderly postural balance while wearing thick socks. STUDY DESIGN: Repeated-measures study design. METHODS: In total, 14 healthy older adults were recruited...
January 1, 2018: Prosthetics and Orthotics International
https://www.readbyqxmd.com/read/29317206/exendin-4-impairs-the-autophagic-flux-to-induce-apoptosis-in-pancreatic-acinar-ar42j-cells-by-down-regulating-lamp-2
#5
Zhiqiang Li, Shaihong Zhu, Lihua Huang, Mingming Shang, Can Yu, Hongwei Zhu, Duo Han, Hui Huang, Xiao Yu, Xia Li
This study aimed to explore the mechanism of impaired autophagy flux induced by exendin-4 and its role on cell apoptosis in pancreatic AR42J cells. The AR42J cells were treated with various concentration of exendin-4 for several time points to assess its cytotoxicity by MTT assay. Then the AR42J cells were treated by 10pM exendin-4 for 72 h, the cell death was analyzed by flow cytometry and caspase-3 level was examined by Western blot with or without the pretreatment of z-VAD-fmk to testify whether exendin-4 induces the cell apoptosis...
January 6, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29305242/allelic-and-haplotype-diversity-of-12-x-strs-in-the-united-arab-emirates
#6
Mohamed A Almarri, Reem A Lootah
X-chromosomal short tandem repeats (X-STRs) are used to complement autosomal and Y-STRs in complex kinship testing, and can be of potential value when determining trace female DNA in male background. We analyzed extracted and directly-amplified samples of 501 males from the United Arab Emirates and report the forensic statistical parameters of 12 X-STRs and haplotypes of the four previously defined linkage groups using the Investigator Argus X-12 QS kit. Combined PDM, PDF, MEC Desmarais Trio and MEC Desmarais Duo based on allele (and haplotype) frequencies were 0...
December 29, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29279761/load-bearing-capacity-of-various-cad-cam-monolithic-molar-crowns-under-recommended-occlusal-thickness-and-reduced-occlusal-thickness-conditions
#7
Sulki Choi, Hyung-In Yoon, Eun-Jin Park
PURPOSE: The goal of this study was to evaluate the fracture resistances of various monolithic crowns fabricated by computer-aided design and computer-aided manufacturing (CAD/CAM) with different thickness. MATERIALS AND METHODS: Test dies were fabricated as mandibular molar forms with occlusal reductions using CAD/CAM. With different occlusal thickness (1.0 or 1.5 mm), a polymer-infiltrated ceramic network (Enamic, EN), and zirconia-reinforced lithium silicate (Suprinity, SU and Celtra-Duo, CD) were used to fabricate molar crowns...
December 2017: Journal of Advanced Prosthodontics
https://www.readbyqxmd.com/read/29239125/autofluorescence-and-white-light-imaging-guided-endoscopic-raman-and-diffuse-reflectance-spectroscopy-for-in-vivo-nasopharyngeal-cancer-detection
#8
Duo Lin, Sufang Qiu, Wei Huang, Jianji Pan, Zhihong Xu, Rong Chen, Shangyuan Feng, Guannan Chen, Yongzeng Li, Michael Short, Jianhua Zhao, Yasser Fawzy, Haishan Zeng
Nasopharyngeal cancer (NPC) is an endemic with high incidence in Southern China and Southeast Asia countries. Screening for NPC under conventional white light imaging (WLI) nasopharyngoscope examination remains a great clinical challenge due to its poor sensitivity. Here, we developed an integrated four-modality endoscopy system combining WLI, autofluorescence imaging (AFI), diffuse reflectance spectroscopy (DRS), and Raman spectroscopy (RS) technologies for in vivo endoscopic cancer detection for the first time...
December 14, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/29232650/internal-validation-of-two-new-retrotransposons-based-kits-innoquant%C3%A2-hy-and-innotyper%C3%A2-21-at-a-forensic-lab
#9
Cátia Martins, Paulo Miguel Ferreira, Raquel Carvalho, Sandra Cristina Costa, Carlos Farinha, Luísa Azevedo, António Amorim, Manuela Oliveira
Obtaining a genetic profile from pieces of evidence collected at a crime scene is the primary objective of forensic laboratories. New procedures, methods, kits, software or equipment must be carefully evaluated and validated before its implementation. The constant development of new methodologies for DNA testing leads to a steady process of validation, which consists of demonstrating that the technology is robust, reproducible, and reliable throughout a defined range of conditions. The present work aims to internally validate two new retrotransposon-based kits (InnoQuant® HY and InnoTyper® 21), under the working conditions of the Laboratório de Polícia Científica da Polícia Judiciária (LPC-PJ)...
November 29, 2017: Forensic Science International
https://www.readbyqxmd.com/read/29231017/-forensic-application-of-huaxiatm-platinum-kit
#10
Y L Wang, X Sheng, M Li, Y L Chen, Y Lin, L Q Chen
OBJECTIVES: To investigate the genetic polymorphism of 23 autosomal STR loci of Huaxia™ Platinum kit in Chinese Han population, and to evaluate the forensic efficiency of Huaxia™ Platinum kit. METHODS: A total of 500 unrelated healthy individuals from Han population were genotyped with Huaxia™ Platinum kit. The frequency distribution and the parameter of population genetics of STR loci were analysed statistically. Huaxia™ Platinum kit was compared with other 7 commercial STR kits commonly seen at home and abroad in the number of STR loci, interior label, fluorescent mark, total number of alleles in Ladder and system effectiveness...
April 2017: Fa Yi Xue za Zhi
https://www.readbyqxmd.com/read/29228024/detection-of-duo-schistosome-infection-from-filtered-urine-samples-from-school-children-in-zambia-after-mda
#11
Megan J Hessler, Austin Cyrs, Steven C Krenzke, El Shaimaa Mahmoud, Chummy Sikasunge, James Mwansa, Nilanjan Lodh
Schistosomiasis is one of the major Neglected Tropical Diseases (NTDs) in sub-Saharan Africa. In sub-Saharan Africa, two major human schistosome species namely Schistosoma mansoni and S. haematobium often occur sympatrically largely affecting children. Recognizing the public health impact of Schistosomiasis, the World Health Organization (WHO) is urging member states to regularly treat at least 75% and up to 100%, of all school-aged children at risk of morbidity. For control strategies based on targeted mass drug administration (MDA) to succeed it is essential to have a simple and sensitive test for monitoring the success of these interventions...
2017: PloS One
https://www.readbyqxmd.com/read/29218337/synthesis-and-photoelectrocatalytic-activity-of-in2o3-hollow-microspheres-via-a-bio-template-route-using-yeast-templates
#12
Duo Pan, Shengsong Ge, Xiyu Zhang, Xianmin Mai, Shengyao Li, Zhanhu Guo
Indium oxide (In2O3) hollow microspheres were prepared using yeast as a bio-template with the aid of a precipitation method. The yeast provided a solid frame for the deposition of In(OH)3 to form the precursor. The resulting In2O3 hollow microspheres were obtained by calcining the precursor at 650 °C. The samples were characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), N2 adsorption-desorption isotherms and UV-Vis diffuse reflectance spectroscopy...
December 8, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29214503/epidemiology-and-clinical-characteristics-of-human-coronaviruses-oc43-229e-nl63-and-hku1-a-study-of-hospitalized-children-with-acute-respiratory-tract-infection-in-guangzhou-china
#13
Zhi-Qi Zeng, De-Hui Chen, Wei-Ping Tan, Shu-Yan Qiu, Duo Xu, Huan-Xi Liang, Mei-Xin Chen, Xiao Li, Zheng-Shi Lin, Wen-Kuan Liu, Rong Zhou
Human coronaviruses (HCoV) OC43, 229E, NL63, and HKU1 are common respiratory viruses which cause various respiratory diseases, including pneumonia. There is a paucity of evidence on the epidemiology and clinical manifestations of these four HCoV strains worldwide. We collected 11,399 throat swabs from hospitalized children with acute respiratory tract infection from July 2009 to June 2016 in Guangzhou, China. These were tested for four strains of HCoV infection using real-time polymerase chain reaction (PCR)...
December 6, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29207749/cirrhosis-of-liver-and-diabetes-mellitus-the-diabolic-duo
#14
Thazhath Mavali Ramachandran, Aninchent Harayil Rajan Rajneesh, George Sarin Zacharia, Rajendran P Adarsh
Introduction: Cirrhosis of the liver and diabetes mellitus are two chronic illnesses with significant impact on the quality of life. Studies from different part of the world have shown the combination to be associated with higher incidence of complications of cirrhosis and reduced survival. However, data on the impact of pre-cirrhotic and post-cirrhotic diabetes on cirrhosis is minimal. Aim: The aim of the study was to determine the complications of cirrhosis patients with and without co-existent DM and to compare the relation between cirrhosis patients with antecedent DM and hepatogenous DM...
September 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/29200541/the-development-and-validation-of-the-closed-set-mandarin-sentence-cms-test
#15
Duo-Duo Tao, Qian-Jie Fu, John J Galvin, Ya-Feng Yu
Matrix-styled sentence tests offer a closed-set paradigm that may be useful when evaluating speech intelligibility. Ideally, sentence test materials should reflect the distribution of phonemes within the target language. We developed and validated the Closed-set Mandarin Sentence (CMS) test to assess Mandarin speech intelligibility in noise. CMS test materials were selected to be familiar words and to represent the natural distribution of vowels, consonants, and lexical tones found in Mandarin Chinese. Ten key words in each of five categories (Name, Verb, Number, Color, and Fruit) were produced by a native Mandarin talker, resulting in a total of 50 words that could be combined to produce 100,000 unique sentences...
September 2017: Speech Communication
https://www.readbyqxmd.com/read/29192293/digital-evaluation-of-the-fit-of-zirconia-reinforced-lithium-silicate-crowns-with-a-new-three-dimensional-approach
#16
Moritz Zimmermann, Andre Valcanaia, Gisele Neiva, Albert Mehl, Dennis Fasbinder
OBJECTIVE: Several methods for the evaluation of fit of computer-aided design/computer-assisted manufacture (CAD/CAM)-fabricated restorations have been described. In the study, digital models were recorded with an intraoral scanning device and were measured using a new three-dimensional (3D) computer technique to evaluate restoration internal fit. The aim of the study was to evaluate the internal adaptation and fit of chairside CAD/CAM-fabricated zirconia-reinforced lithium silicate ceramic crowns fabricated with different post-milling protocols...
November 30, 2017: Quintessence International
https://www.readbyqxmd.com/read/29167687/effect-of-self-adhesive-and-separate-etch-adhesive-dual-cure-resin-cements-on-the-bond-strength-of-fiber-post-to-dentin-at-different-parts-of-the-root
#17
Ehsan Mohamadian Amiri, Fariba Balouch, Faezeh Atri
Objectives: Bonding of fiber posts to intracanal dentin is challenging in the clinical setting. This study aimed to compare the effect of self-adhesive and separate etch adhesive dual cure resin cements on the bond strength of fiber post to dentin at different parts of the root. Materials and Methods: This in-vitro experimental study was conducted on 20 single-rooted premolars. The teeth were decoronated at 1mm coronal to the cementoenamel junction (CEJ), and the roots underwent root canal treatment...
May 2017: Journal of Dentistry of Tehran University of Medical Sciences
https://www.readbyqxmd.com/read/29166421/wear-and-fracture-strength-of-new-ceramic-resins-for-chairside-milling
#18
Karina Zierden, Johannes Acar, Peter Rehmann, Bernd Wöstmann
PURPOSE: The purpose of this in vitro study was to evaluate wear and fracture strength of chairside-milled new ceramic resins after artificial aging in a chewing simulator. MATERIALS AND METHODS: Two ceramic resins (Lava Ultimate and Vita Enamic) and two conventional ceramics (IPS Empress CAD and Celtra Duo) were tested. For analysis of wear, baseline and follow-up scans of the specimens were made, the scans were superimposed, and the wear was calculated. To determine fracture strength, the specimens were loaded until they fractured...
November 22, 2017: International Journal of Prosthodontics
https://www.readbyqxmd.com/read/29157995/in-the-ovine-pituitary-cxcr4-is-localized-in-gonadotropes-and-somatotropes-and-increases-with-elevated-serum-progesterone
#19
N S Sanchez, K E Quinn, A K Ashley, R L Ashley
The pituitary is the central endocrine regulator of reproduction and in addition to various hormones regulating its actions, other molecules, such as chemokines, influence pituitary physiology as well. Despite reports over 2 decades ago that chemokines regulate the pituitary, much of the basic biology discerning chemokine action in the pituitary is unclear. A small number of chemokines and their receptors have been localized to the pituitary, yet chemokine ligand 12 (CXCL12) and its receptor, CXCR4, have received the most attention as both are increased in human pituitary adenomas...
October 28, 2017: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/29128964/in-vivo-assessment-of-a-novel-biodegradable-ureteral-stent
#20
Alexandre A Barros, Carlos Oliveira, Ana J Ribeiro, Riccardo Autorino, Rui L Reis, Ana Rita C Duarte, Estevão Lima
PURPOSE: To perform an in vivo assessment of a newly developed biodegradable ureteral stent (BUS) produced with natural-based polymers. METHODS: The BUS is based on a patented technology combining the injection process with the use of supercritical fluid technology. Study was conducted at ICVS-University of Minho (Braga, Portugal) and a total of ten domestic pigs were used. In seven animals, the experimental BUS stent was inserted, whereas in the remaining a commercially available stent was used (6-Fr Biosoft(®) duo stents, Porges Coloplast, Denmark)...
November 11, 2017: World Journal of Urology
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