Maurizio Bruschi, Simona Granata, Laura Santucci, Giovanni Candiano, Antonia Fabris, Nadia Antonucci, Andrea Petretto, Martina Bartolucci, Genny Del Zotto, Francesca Antonini, Gian Marco Ghiggeri, Antonio Lupo, Giovanni Gambaro, Gianluigi Zaza
BACKGROUND AND OBJECTIVES: Microvesicles and exosomes are involved in the pathogenesis of autosomal dominant polycystic kidney disease. However, it is unclear whether they also contribute to medullary sponge kidney, a sporadic kidney malformation featuring cysts, nephrocalcinosis, and recurrent kidney stones. We addressed this knowledge gap by comparative proteomic analysis. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: The protein content of microvesicles and exosomes isolated from the urine of 15 patients with medullary sponge kidney and 15 patients with autosomal dominant polycystic kidney disease was determined by mass spectrometry followed by weighted gene coexpression network analysis, support vector machine learning, and partial least squares discriminant analysis to compare the profiles and select the most discriminative proteins...
June 7, 2019: Clinical Journal of the American Society of Nephrology: CJASN