Yaru Zhou, Xuezhang Tian, Shaowei Wang, Ming Gao, Chuchu Zhang, Jiali Ma, Xi Cheng, Lei Bai, Hai-Bin Qin, Min-Hua Luo, Qingsong Qin, Baishan Jiang, Ke Lan, Junjie Zhang
Kaposi's sarcoma-associated herpesvirus (KSHV) is a double-stranded DNA virus etiologically associated with multiple malignancies. Both latency and sporadic lytic reactivation contribute to KSHV-associated malignancies, however, the specific roles of many KSHV lytic gene products in KSHV replication remains elusive. In this study, we report that ablation of ORF55, a late gene encoding a tegument protein, does not impact KSHV lytic reactivation but significantly reduces the production of progeny virions. We found that cysteine 10 and 11 (C10 and C11) of pORF55 are palmitoylated, and the palmytoilation is essential for its Golgi localization and secondary envelope formation...
April 16, 2024: PLoS Pathogens