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https://www.readbyqxmd.com/read/29331373/establishment-and-characterization-of-five-immortalized-human-scalp-dermal-papilla-cell-lines
#1
Mi Hee Kwack, Jung Min Yang, Gong Hee Won, Moon Kyu Kim, Jung Chul Kim, Young Kwan Sung
Dermal papilla (DP) regulates the growth and cycling of hair follicles. Cultured DP cells are useful for the study of their role in relation to hair growth and regeneration. However, cultivation of human DP cells is tedious and difficult. In addition, cultured DP cells possess a relatively short replicative life span, requiring immortalized human DP cell lines. We previously established an immortalized human DP cell line, SV40T-hTERT-DPC, by introducing human telomerase reverse transcriptase (hTERT) gene into the transformed cell line, SV40T-DPC...
January 10, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29330844/bradykinin-mediates-the-association-of-collecting-duct-cells-to-form-migratory-colonies-through-b2-receptor-activation
#2
Edith Del Valle Guaytima, Yamila Romina Brandán, Nicolás Octavio Favale, Bruno Jaime Santacreu, Norma B Sterin-Speziale, María Gabriela Márquez
It is known that bradykinin (BK) B2 receptor (B2R) is expressed in the collecting duct (CD) cells of the newborn rat kidney, but little is known about its role during early postnatal life. Therefore, we hypothesize that BK could participate in the mechanisms that mediate CD formation during the postnatal renal development. Performing primary cultures, combined with biochemical, immunocytochemical, and time-lapse analysis, we studied the role of BK in CD cell behaviour isolated from renal papilla of neonatal rats...
January 13, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29321056/mri-appearance-of-ovarian-serous-borderline-tumors-of-the-micropapillary-type-compared-to-that-of-typical-ovarian-serous-borderline-tumors-radiologic-pathologic-correlation
#3
Go Nakai, Takashi Yamada, Kazuhiro Yamamoto, Yoshinobu Hirose, Masahide Ohmichi, Yoshifumi Narumi
BACKGROUND: Serous borderline tumor (SBT) of the micropapillary type (SBT-MP) became one of the major pathological SBT diagnoses in addition to typical SBT, and was also defined as "non-invasive" low-gradeserous carcinoma according to the World Health Organization (WHO) classification in 2014. In this study, we investigated the MRI appearance of SBT-MP compared to that of typical SBT in order to identify specific imaging features of SBT-MP that correspond to pathological findings. METHODS: MR images of 6 histologically proven ovarian SBT-MP in four patients and 14 typical SBT in ten patients were reviewed retrospectively...
January 10, 2018: Journal of Ovarian Research
https://www.readbyqxmd.com/read/29320823/first-blindness-cases-of-horses-infected-with-setaria-digitata-nematoda-filarioidea-in-the-republic-of-korea
#4
Jihun Shin, Kyu-Sung Ahn, Guk-Hyun Suh, Ha-Jung Kim, Hak-Sub Jeong, Byung-Su Kim, Eunsang Choi, Sung-Shik Shin
Ocular setariases of cattle were reported but those of equine hosts have never been reported in the Republic of Korea (Korea). We found motile worms in the aqueous humor of 15 horses (Equus spp.) from 12 localities in southern parts of Korea between January 2004 and November 2017. After the affected animals were properly restrained under sedation and local anesthesia, 10 ml disposable syringe with a 16-gauge needle was inserted into the anterior chamber of the affected eye to successfully remove the parasites...
December 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/29320372/a-dentin-derived-hydrogel-bioink-for-3d-bioprinting-of-cell-laden-scaffolds-for-regenerative-dentistry
#5
Avathamsa Athirasala, Anthony Tahayeri, Greeshma Thrivikraman, Cristiane M França, Nelson Monteiro, Victor Tran, Jack Ferracane, Luiz E Bertassoni
Recent studies in tissue engineering have adopted extracellular matrix (ECM) derived scaffolds as natural and cytocompatible microenvironments for tissue regeneration. The dentin matrix, specifically, has been shown to be associated with a host of soluble and insoluble signaling molecules that can promote odontogenesis. Here, we have developed a novel bioink, blending printable alginate (3% w/v) hydrogels with the soluble and insoluble fractions of the dentin matrix. We have optimized the printing parameters and the concentrations of the individual components of the bioink for print accuracy, cell viability and odontogenic potential...
January 10, 2018: Biofabrication
https://www.readbyqxmd.com/read/29318579/evaluation-of-icam-1-expression-and-vascular-changes-in-the-skin-of-patients-with-plaque-pustular-and-erythrodermic-psoriasis
#6
Aline L Bressan, Bruna L S Picciani, Luna Azulay-Abulafia, Anna K Fausto-Silva, Paula N Almeida, Karin S G Cunha, Eliane P Dias, Sueli Carneiro
BACKGROUND: Psoriasis is a chronic inflammatory disease. Pustular, erythrodermic, and extensive plaque psoriasis are responsible for systemic complications. Systemic capillary leak syndrome is the complication with greater progression to death and occurs due to vascular changes. PURPOSE: The aim of this study was to evaluate vascular changes through the expression of CD34 and ICAM-1 in plaque, pustular, and erythrodermic psoriasis. METHODS: The sample consisted of seven patients with erythrodermic psoriasis, 24 with moderate-severe plaque psoriasis, 14 with mild plaque psoriasis and 13 with pustular psoriasis...
January 10, 2018: International Journal of Dermatology
https://www.readbyqxmd.com/read/29317269/the-impact-of-wire-caliber-on-ercp-outcomes-a-multicenter-randomized-controlled-trial-of-0-025-inch-and-0-035-inch-guidewires
#7
Milan S Bassan, Praka Sundaralingam, Scott B Fanning, James Lau, Jayaram Menon, Evan Ong, Rungsun Rerknimitr, Dong-Wan Seo, Eng Kiong Teo, Hsiu-Po Wang, D Nageshwar Reddy, Khean Lee Goh, Michael J Bourke
BACKGROUND AND AIMS: Wire-guided biliary cannulation has been demonstrated to improve cannulation rates and reduce post-ERCP pancreatitis (PEP), but the impact of wire caliber has not been studied. This study compares successful cannulation rates and ERCP adverse events using a 0.025-inch and 0.035-inch guidewire. METHODS: A randomized, single blinded, prospective multi-center trial at 9 high volume tertiary referral centers in the Asia Pacific region was performed...
January 6, 2018: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/29317267/spiral-enteroscopy-assisted-ercp-in-bariatric-length-roux-en-y-anatomy-a-large-single-center-series-and-review-of-the-literature-with-video
#8
Mohammad F Ali, Rani Modayil, Krishna C Gurram, Collin Brathwaite, David Friedel, Stavros Stavropoulos
BACKGROUND: Deep enteroscopy-assisted ERCP (DEA-ERCP) in post-bariatric Roux-en-Y (RY) anatomy is challenging. Laparoscopy-assisted ERCP (LA-ERCP) or EUS-directed transgastric ERCP (EDGE) are technically easier and faster, but are more invasive and morbid procedures. Therefore, we have used DEA-ERCP as our first-line approach, reserving EDGE and LA-ERCP for cases in which adjunctive techniques that cannot be performed through an enteroscope are required (eg, EUS-FNA, SO manometry), or DEA-ERCP failures...
January 6, 2018: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/29317265/wound-regeneration-deficit-in-rats-correlates-with-low-morphogenetic-potential-and-distinct-transcriptome-profile-of-epidermis
#9
Christian F Guerrero-Juarez, Aliaksandr A Astrowski, Rabi Murad, Christina T Dang, Vera O Shatrova, Aksana Astrowskaja, Chae Ho Lim, Raul Ramos, Xiaojie Wang, Yuchen Liu, Hye-Lim Lee, Kim T Pham, Tsai-Ching Hsi, Ji Won Oh, Daniel Crocker, Ali Mortazavi, Mayumi Ito, Maksim V Plikus
Large excisional wounds in mice prominently regenerate new hair follicles (HFs) and fat, yet humans are deficient for this regenerative behavior. Currently, wound-induced regeneration remains a clinically desirable, but only partially understood phenomenon. We show that large excisional wounds in rats, across seven strains fail to regenerate new HFs. We compared wound transcriptomes between mice and rats at the time of scab detachment, which coincides with the onset of HF regeneration in mice. In both species, wound dermis and epidermis share core dermal and epidermal transcriptional programs respectively, yet prominent inter-species differences exist...
January 6, 2018: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/29306957/analysis-of-taste-function-in-patients-before-and-after-cochlear-implant-surgery
#10
U Walliczek-Dworschak, C M Knauer, D Murbe, A Mainka, T Hummel
BACKGROUND: Cochlear implantation (CI), the standard therapy for rehabilitation of severe to profound sensorineural hearing loss, exhibits taste dysfunction as a possible postoperative complication. This study aimed to investigate possible postoperative changes in taste and in addition baseline taste function in CI candidates. METHODOLOGY: We included 61 patients (32 male, 29 female) planned to undergo CI surgery and 23 healthy controls (8 male, 15 female). Gustatory function was measured using a chemosensory quasi-threshold test (taste strips) and electrogustometry (EGM)...
January 7, 2018: Rhinology
https://www.readbyqxmd.com/read/29303760/a-modified-frenectomy-technique-a-new-surgical-approach
#11
Mahdi Kadkhodazadeh, Reza Amid, Mehdi Ekhlasmand Kermani, Sepanta Hosseinpour
The frenum is a mucosal fold that attaches the lips or cheeks to the alveolar mucosa, gingiva, and underlying periosteum. Consequences of an abnormal frenal attachment include gingival recession, decreased vestibular depth, decreased range of lip movement, and involvement of interdental papilla, causing a diastema. Several methods to eliminate ectopic frenal attachments have been suggested, including frenectomy (elimination) and frenotomy (repositioning). This case report describes the use of a modified frenectomy technique in a 15-year-old girl with excess gingiva between the maxillary central incisors, which exhibited a 3-mm diastema...
January 2018: General Dentistry
https://www.readbyqxmd.com/read/29295755/regional-morphology-and-mucus-composition-in-the-urogenital-papilla-skin-of-the-blackbelly-rosefish-helicolenus-dactylopterus-delaroche-1809
#12
Gianluca Accogli, Letizia Sion, Porzia Maiorano, Francesca Capezzuto, Gianfranco D'Onghia, Salvatore Desantis
Blackbelly rosefish Helicolenus dactylopterus is a zygoparous fish whose males are equipped with the copulating organ named urogenital papilla (UP). This study deals with the morphology and the glycoconjugate pattern of the UP epidermis, which is the male tissue interacting with the female internal body during copulation. The carbohydrate content was studied by means of conventional and lectin histochemistry. The epidermis was shown to be a stratified cuboidal epithelium and to exhibit characteristic intraepithelial pits in the apical zone...
December 26, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29295567/minoxidil-induction-of-vegf-is-mediated-by-inhibition-of-hif-prolyl-hydroxylase
#13
Soohwan Yum, Seongkeun Jeong, Dohoon Kim, Sunyoung Lee, Wooseong Kim, Jin-Wook Yoo, Jung-Ae Kim, Oh Sang Kwon, Dae-Duk Kim, Do Sik Min, Yunjin Jung
The topical application of minoxidil may achieve millimolar concentrations in the skin. We investigated whether millimolar minoxidil could induce vascular endothelial growth factor (VEGF), a possible effector for minoxidil-mediated hair growth, and how it occurred at the molecular level. Cell-based experiments were performed to investigate a molecular mechanism underlying the millimolar minoxidil induction of VEGF. The inhibitory effect of minoxidil on hypoxia-inducible factor (HIF) prolyl hydroxylase-2 (PHD-2) was tested by an in vitro von Hippel-Lindau protein (VHL) binding assay...
December 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29285254/microrna-co-expression-networks-exhibit-increased-complexity-in-pancreatic-ductal-compared-to-vater-s-papilla-adenocarcinoma
#14
Tommaso Mazza, Massimiliano Copetti, Daniele Capocefalo, Caterina Fusilli, Tommaso Biagini, Massimo Carella, Antonio De Bonis, Nicola Mastrodonato, Ada Piepoli, Valerio Pazienza, Evaristo Maiello, Fabio Francesco di Mola, Pierluigi di Sebastiano, Angelo Andriulli, Francesca Tavano
MiRNA expression abnormalities in adenocarcinoma arising from pancreatic ductal system (PDAC) and Vater's papilla (PVAC) could be associated with distinctive pathologic features and clinical cancer behaviours. Our previous miRNA expression profiling data on PDAC (n=9) and PVAC (n=4) were revaluated to define differences/similarities in miRNA expression patterns. Afterwards, in order to uncover target genes and core signalling pathways regulated by specific miRNAs in these two tumour entities, miRNA interaction networks were wired for each tumour entity, and experimentally validated target genes underwent pathways enrichment analysis...
December 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/29280232/intraspecific-variation-in-seed-morphology-of-tribe-vicieae-papilionoidae-using-scanning-electron-microscopy-techniques
#15
Neelam Rashid, Muhammad Zafar, Mushtaq Ahmad, Khafsa Malik, Ihsan-Ul Haq, Syed Nasar Shah, Abdul Mateen, Tauseef Ahmed
Seed micromorphology of 12 species of tribe Vicieae (Papilionoidae) representing five genera were examined using Scanning Electron Microscope (SEM). The different seed types were described, illustrated, compared, and their taxonomic importance is discussed. Seeds exhibit great diversity in ultrastructure and a variety of novel morphological features have been determined among and within genera of the tribe. Seeds were characterized by oval to round shape, ovate to oblong outline and striate to papillate ornamentation...
December 27, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/29279573/effect-of-local-bone-marrow-stromal-cell-administration-on-ligature-induced-periodontitis-in-mice
#16
Shinya Iguchi, Daigo Suzuki, Eisuke Kawano, Takayuki Mashimo, Mikihito Kajiya, Taku Toriumi, Toshihisa Kawai, Hidemi Kurihara, Keitaro Isokawa, Shuichi Sato, Masaki Honda
Bone marrow-derived multipotent stromal cells (BMSCs) have potent antiinflammatory effects. This study aimed to investigate the antiinflammatory potential of BMSCs using a mouse model of ligature-induced periodontitis. BMSCs were isolated from the femurs and tibiae of mice. Periodontitis was induced by placing a ligature around the right maxillary second molar. After 3 days, the mice were administered BMSC in the gingiva of the mesial interdental papilla around the ligatured molar. The ligatured and non-ligatured mice that were not administered BMSC served as controls...
2017: Journal of Oral Science
https://www.readbyqxmd.com/read/29279152/mri-features-and-score-for-differentiating-borderline-from-malignant-epithelial-ovarian-tumors
#17
Yong Ai Li, Jin Wei Qiang, Feng Hua Ma, Hai Ming Li, Shu Hui Zhao
PURPOSE: To identify the MRI features of borderline epithelial ovarian tumors (BEOTs) and to differentiate BEOTs from malignant epithelial ovarian tumors (MEOTs). MATERIALS AND METHODS: The clinical and MRI data of 89 patients with a BEOT and 109 patients with a MEOT proven by surgery and histopathology were retrospectively reviewed. MRI features, including bilaterality, size, shape, margin, cystic-solid interface, configuration, papillae or nodules, signal intensity, enhancement, presence of an ipsilateral ovary, peritoneal implants and ascites were analyzed and compared...
January 2018: European Journal of Radiology
https://www.readbyqxmd.com/read/29278699/perinatal-development-of-bitter-taste-avoidance-behavior-in-mice-is-associated-with-actin-dependent-localization-of-bitter-taste-receptors-to-the-microvilli-of-taste-cells
#18
Atsuko Yamashita, Kaori Kondo, Yoshimi Kunishima, Sachiko Iseki, Takashi Kondo, Masato S Ota
Bitter taste avoidance behavior (BAB) plays a fundamental role in the avoidance of toxic substances with a bitter taste. However, the molecular basis underlying the development of BAB is unknown. To study critical developmental events by which taste buds turn into functional organs with BAB, we investigated the early phase development of BAB in postnatal mice in response to bitter-tasting compounds, such as quinine and thiamine. Postnatal mice started to exhibit BAB for thiamine and quinine at postnatal day 5 (PD5) and PD7, respectively...
December 23, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29278000/capsule-endoscopy-with-pillcamsb2-versus-pillcamsb3-has-the-improvement-in-technology-resulted-in-a-step-forward
#19
Sofia Xavier, Sara Monteiro, Joana Magalhães, Bruno Rosa, Maria João Moreira, José Cotter
AIM: To compare the findings and completion rate of PillCam® SB2 and SB3. METHODS: This was a retrospective single-center study that included 357 consecutive small bowel capsule endoscopies (SBCE), 173 SB2 and 184 SB3. The data collected included age, gender, capsule type (PillCam® SB2 or SB3), quality of bowel preparation, completion of the examination, gastric and small bowel transit time, small bowel findings, findings in segments other than the small bowel and the detection of specific anatomical markers, such as the Z line and papilla...
December 26, 2017: Revista Española de Enfermedades Digestivas
https://www.readbyqxmd.com/read/29275854/edta-treatment-for-sodium-hypochlorite-treated-dentin-recovers-disturbed-attachment-and-induces-differentiation-of-mouse-dental-papilla-cells
#20
Kentaro Hashimoto, Nobuyuki Kawashima, Shizuko Ichinose, Keisuke Nara, Sonoko Noda, Takashi Okiji
INTRODUCTION: The disturbance of cellular attachment to dentin by sodium hypochlorite (NaOCl) may hamper pulp tissue regeneration. The aims of this study were to examine the recovering effect of EDTA on the attachment/differentiation of stemlike cells and to address the mechanisms of EDTA-induced recovery under the hypothesis that attachment to the exposed dentin matrix and the subsequent activation of integrin/phosphatidylinositol 3-kinase (PI3K) signaling play a crucial role. METHODS: Mouse dental papilla (MDP) cells were cultured on bovine dentin disks treated with NaOCl (0%, 1...
December 21, 2017: Journal of Endodontics
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